| the linkage of diaminopimelic acid in the peptidoglycan of bacillus megaterium nctc 7581. | | 1969 | 4244235 |
| the growth of cells of bacillus megaterium in a hypertonic phosphate buffer. | | 1971 | 4251884 |
| peptidoglycan synthesis in bacilli. i. effect of temperature on the in vitro system from bacillus megaterium and bacillus stearothermophilus. | | 1971 | 4255188 |
| turnover of mucopeptide during the life cycle of bacillus megaterium. | | 1971 | 4256604 |
| purification and properties of atpase from the cytoplasmic membrane of bacillus megaterium km. | | 1971 | 4258592 |
| breakdown of pulse-labeled ribonucleic acid in bacillus megaterium, revealed by exposure to the antibiotics mithramycin, chromomycin, and nogalamycin. | | 1972 | 4258648 |
| alternative purification of the membrane-bound atpase from bacillus megaterium km, and some properties. | | 1972 | 4261812 |
| penicillin-sensitive transpeptidation during peptidoglycan biosynthesis in cell-free preparations from bacillus megaterium. i. incorporation of free diaminopimelic acid into peptidoglycan. | | 1972 | 4262449 |
| penicillin-sensitive transpeptidation during peptidoglycan biosynthesis in cell-free preparations from bacillus megaterium. ii. effect of penicillins and cephalosporins on bacterial growth and in vitro transpeptidation. | | 1972 | 4262450 |
| spore germination of bacillus megaterium qm b1551 mutants. | auxotrophic or antibiotic-resistant mutants of bacillus megaterium qm b1551 are capable of initiation of germination similar to the parental (qm b1551) prototrophic strain. | 1972 | 4263407 |
| molecular weight, amino acid composition and other properties of membrane-bound atpase from bacillus megaterium km. | | 1973 | 4266162 |
| antibodies to purified membrane-bound atpase from bacillus megaterium km and their reaction with protoplasts and cytoplasmic membranes. | | 1974 | 4275606 |
| effect of polymyxin b, tyrocidine, gramicidin d, and other antibiotics on the enzymatic hydrolysis of poly-beta-hydroxybutyrate. | merrick, j. m. (state university of new york, buffalo). effect of polymyxin b, tyrocydine, gramicidin d, and other antibiotics on the enzymatic hydrolysis of poly-beta-hydroxybutyrate. j. bacteriol. 90:965-969. 1965.-previous studies have demonstrated that native poly-beta-hydroxybutyrate (phb) granules isolated from bacillus megaterium are surrounded by a discrete membranelike structure. morphological alterations of phb granules are mainly characterized by membrane fragmentation, and can be cor ... | 1965 | 4284918 |
| electron microscopic study on the effect of the oxidation of ultrathin sections of bacillus cereus and bacillus megaterium. | | 1967 | 4290538 |
| the cytochrome system of bacillus megaterium km. the presence and some properties of two co-binding cytochromes. | | 1967 | 4292158 |
| electron microscopic observations on the sites of activities of succinic dehydrogenase and cytochrome oxidase in mycobacterium tuberculosis and bacillus megaterium. | | 1966 | 4293572 |
| isomers of glucosaminylphospatiylglycerol in bacillus megaterium. | 1. the lipids of bacillus megaterium were extracted and three lipids containing glucosamine were identified. one of these is not a phospholipid, but the other two, which differ in their chromatographic behaviour, contain phosphorus, glycerol, fatty acid and d-glucosamine in the molar proportions 1:2:2:1. 2. in both phosphoglycolipids, the fatty acids are bound in ester linkage, and both yield 2,5-anhydromannose and 3-sn-phosphatidyl-1'-sn-glycerol on treatment with sodium nitrite. 3. both phosph ... | 1969 | 4309309 |
| on the role of quinones in bacterial electron transport. the respiratory system of bacillus megaterium. | | 1969 | 4311782 |
| masking of bacillus megaterium km membrane reduced nicotinamide adenine dinucleotide oxidase and solubilization studies. | solubilization of a reduced nicotinamide adenine dinucleotide (nadh)-2,6 dichlorophenol indophenol (dcip) oxidoreductase associated with the membrane nadh oxidase system of bacillus megaterium km was effected by treatment with 0.2% sodium deoxycholate, 8 m urea, or buffer (ph 9.0) in the presence of ethyl-enediaminetetraacetate. these treatments inactivated membrane nadh oxidase. it was found that membrane nadh oxidase and nadh-dcip oxidoreductase were masked in membranes. several procedures, in ... | 1970 | 4314476 |
| respiratory chain of a pathogenic fungus, microsporum gypseum: effect of the antifungal agent pyrrolnitrin. | pyrrolnitrin has been reported to inhibit bacillus megaterium primarily by forming complexes with phospholipids and to block electron transfer of saccharomyces cerevisiae between succinate or reduced nicotinamide adenine dinucleotide (nadh) and coenzyme q. we found that pyrrolnitrin inhibited respiration of conidia of microsporum gypseum. in mitochondrial preparations, pyrrolnitrin strongly inhibited respiration and the rotenone-sensitive nadh-cytochrome c reductase. the rotenone-insensitive nad ... | 1971 | 4323963 |
| antimicrobial actions of hexachlorophene: release of cytoplasmic materials. | intracellular solutes were released from growing or resting cells of bacillus megaterium as a consequence of hexachlorophene treatment. the effect was dose dependent, with the optimum at a concentration about sevenfold greater than the minimal lethal dose. the effects of ph and temperature on the leakage process also were inconsistent with the killing effects of the drug. the types of materials released appeared to be the same with or without hexachlorophene treatment. the released materials wer ... | 1971 | 4330741 |
| properties of mesosomal membranes isolated from micrococcus lysodeikticus and bacillus megaterium. | | 1971 | 4331581 |
| structure of schizokinen, an iron-transport compound from bacillus megaterium. | | 1971 | 4332462 |
| separation of the primary dehydrogenase from the cytochromes of the nicotinamide adenine dinucleotide (reduced form) oxidase of bacillus megaterium. | a selective extraction procedure was developed for sequentially extracting a fraction containing the primary dehydrogenase and a fraction containing the cytochromes of the nicotinamide adenine dinucleotide (reduced form) (nadh) oxidase of bacillus megaterium km membranes. the primary dehydrogenase (nadh-2,6-dichlorophenolindophenol oxidoreductase) activity was extracted from sonically treated membranes with 0.4% sodium deoxycholate for 30 min at 4 c. the insoluble residue was extracted with 0.4% ... | 1972 | 4333382 |
| inability of detect cyclic amp in vegetative or sporulating cells or dormant spores of bacillus megaterium. | | 1973 | 4351135 |
| pencillin amidase production by bacillus megaterium. | | 1973 | 4353385 |
| properties of a thermosensitive asporogenous filamentous mutant of bacillus megaterium. | mutant th14 of bacillus megaterium atcc 19213 is thermosensitive and defective in cell-division septation and spore formation at the restrictive temperature (39 c). as a consequence, the mutant forms multinucleate aseptate filaments and is asporogenic. the mutation does not result in any qualitative compositional changes in extractable membrane proteins. at the restrictive temperature, the mutant membrane has a reduced content of a small molecular weight protein(s). a membrane protein(s) with a ... | 1974 | 4364329 |
| [septum formation in sporogenic and asporogenic bacillus megaterium cells during transition to nitrogen- and carbon starvation]. | | 1974 | 4368253 |
| glucose dehydrogenase of bacillus megaterium km. coupling of the cytoplasmic enzyme with membrane-bound cytochromes. | | 1969 | 4388314 |
| biochemical studies of bacterial sporulation and germination. xxii. energy metabolism in early stages of germination of bacillus megaterium spores. | | 1970 | 4394282 |
| the effect of temperature and of cetrimide on the rate of loss of refractility of spores of bacillus megaterium. | | 1971 | 4395904 |
| [glutamate cycle, first step of nitrogen metabolism in bacillus megaterium]. | | 1972 | 4403243 |
| the recovery of bacillus megaterium spores from white soft paraffin. | | 1972 | 4403756 |
| isocitrate lyase from a thermophilic bacillus: effect of salts on enzyme activity. | the isocitrate lyase from a thermophilic bacillus is activated about threefold by a variety of salts. such strong stimulation of activity is not seen with isocitrate lyase from the mesophiles, bacillus licheniformis, bacillus megaterium, escherichia coli, and aspergillus nidulans. the salt activation is markedly ph-dependent. at ph values above 8.6, salt (kcl) indeed inhibits the enzyme activity. potassium chloride also causes a significant shift of the ph optimum of the enzyme towards the acid ... | 1973 | 4584800 |
| germination of spores of bacillus stearothermophilus induced by analogues of dipicolinate di-anion. | the structural specificity required for induction of germination of spores of bacillus stearothermophilus by analogues of dipicolinate in buffer at ph 5.5 is similar to that found previously with spores of bacillus megaterium and calcium chelate salts at ph 8. 4h-pyran-2,6-dicarboxylate, but no other analogue tested, is as effective as dipicolinate di-anion. | 1974 | 4591954 |
| mode of cell wall growth of bacillus megaterium. | the growth of the cell wall of bacillus megaterium km was examined by labeling the cell wall uniformly with (3)h-alpha, epsilon-diaminopimelic acid and examining by radioautography the distribution of labeled cell wall material in daughter cells. the results indicate that the label is distributed uniformly to all daughter cells and rule out a single growth point, either central or apical, for these cells. the results strongly suggest that cell wall growth occurs by uniform deposition of new wall ... | 1972 | 4621629 |
| studies on the cytoplasmic membranes of bacillus megaterium km. i. fractionation of the cytoplasmic membranes by alkali-treatment. | | 1972 | 4622705 |
| effect of mycotoxins and coumarins on the growth of bacillus megaterium from spores. | | 1972 | 4622739 |
| protein turnover in asporogenic bacillus megaterium km under limited nitrogen supply. | | 1972 | 4622790 |
| effect of carbon source on size and associated properties of bacillus megaterium spores. | the size of the spores produced by bacillus megaterium atcc 19213 depended upon the nature of the carbon source present in the defined medium in which they were produced. homogeneous preparations of small (0.38 mum(3)), nearly spherical spores were produced after batch culture in the presence of 2.8 mm citrate, and large (1.17 mum(3)), oblong spores were produced by replacement culture in the presence of 7.35 mm acetate. large and small spores had approximately the same deoxyribonucleic acid con ... | 1972 | 4622903 |
| a phospholipase in bacillus megaterium unique to spores and sporangia. | | 1972 | 4623624 |
| [role of catabolic and anabolic processes in relation to the uneven growth of bacillus megaterium in the exponential growth phase]. | | 1972 | 4625328 |
| [depolymerization, by a controlled chemical process, of beta hydroxybutyric lipid (or phb) of bacillus megaterium]. | | 1972 | 4625354 |
| mode of action of primaquine: preferential inhibition of protein biosynthesis in bacillus megaterium. | the growth of a strain of bacillus megaterium was prevented by a minimal inhibitory concentration of primaquine of 52 mug/ml or 2 x 10(-4)m. when exponentially growing cultures received the drug at 6 x 10(-4)m, the rate of growth was drastically reduced and no further growth occurred after 15 min of exposure. at this concentration, primaquine was bactericidal, causing a 50% reduction in the viable population after one doubling time of 45 min. supplying primaquine to cultures 30 min after adding ... | 1972 | 4625625 |
| spore pool glutamic acid as a metabolite in germination. | spore glutamic acid pools were examined in dormant and germinating spores using colorimetric and (14)c analytical procedures. germination of spores of bacillus megaterium (parent strain), initiated by d-glucose, was accompanied by a rapid drop in the level of spore pool glutamate, from 12.0 mug/mg of dry spores to 7.7 mug/mg of dry spores after 30 sec of germination. similar decreases in extractable spore pool glutamate were observed with l-alanine-initiated germination of b. licheniformis spore ... | 1972 | 4626499 |
| minimal requirements for commitment to sporulation in bacillus megaterium. | commitment to sporulation was examined by means of both endotrophic sporulation and rejuvenation experiments. in both cases, a point of commitment to the completion of sporulation occurred at stage ii. with 2 x 10(8) cells/ml, the process by which commitment occurred required 0.5 mm mg(2+), 10.0 mm phosphate, and an energy source (minimum of 5.0 mm acetate). for completion of sporulation and formation of normal, heat-resistant refractile spores, 0.3 mm ca(2+) was required. except for this ca(2+) ... | 1972 | 4626503 |
| appearance of muramic lactam during cortex synthesis in sporulating cultures of bacillus cereus and bacillus megaterium. | evidence is presented for the appearance of muramic lactam during the late stages of sporulation at about the same time dipicolinic acid synthesis occurs. | 1972 | 4626506 |
| purification of n-acetylmuramic acid-l-alanine amidase from bacillus megaterium. | | 1972 | 4626719 |
| regulation of the formation of proteinases in bacillus megaterium. v. characterization of two megaterioproteinases differing in the control of their synthesis. | | 1972 | 4627015 |
| utilization of collagenous by-products from the meat packing industry: production of single-cell protein by the continuous cultivation of bacillus megaterium. | the conditions for continuous cultivation of bacillus megaterium on a collagen-derived substrate (sp-100) were determined. the optimum conditions of temperature, ph, and dilution rate were 34 c, ph 7.0, and 0.25/hr, respectively. increasing the substrate concentration in plain tap water resulted in proportional increases in the productivity of cell mass from 0.6 g per liter per hr at 1% substrate to 1.8 g per liter per hr at 10% substrate; however, the protein content of the biomass decreased fr ... | 1972 | 4627231 |
| reversal by a specific peptide (diacetyl-alpha gamma-l-diaminobutyryl-d-alanyl-d-alanine) of vancomycin inhibition in intact bacteria and cell-free preparations. | vancomycin inhibited the growth of bacillus megaterium, staphylococcus aureus and micrococcus lysodeikticus, and in cell-free preparations from b. megaterium it inhibited the formation of mucopeptide and enhanced the accumulation of the lipid intermediate in the biosynthetic pathway. all these inhibitory processes were reversed by the presence of a synthetic peptide analogous to un-cross-linked mucopeptide side chains, namely diacetyl-l-diaminobutyryl-d-alanyl-d-alanine. a considerable amount of ... | 1972 | 4627581 |
| 1-(o- -glucosaminyl)-2,3-diglyceride in bacillus megaterium. | 1. a lipid that contains glucosamine but not phosphorus has been isolated from bacillus megaterium. it constitutes about 5% of the total lipid glucosaminide in this organism and can be distinguished chromatographically from 2'-(o-beta-glucosaminyl)phosphatidylglycerol and 3'-(o-beta-glucosaminyl)phosphatidylglycerol, which are also present. 2. the lipid contains glycerol, fatty acids and glucosamine in the molar proportion 1:2:1. the fatty acids are bound by an ester linkage and are similar to t ... | 1972 | 4627584 |
| the microbial biosynthesis of methionine. | 1. the enzymes leading to the methylation of homocysteine have been examined in three micro-organisms: a cobalamin-producing bacterium, bacillus megaterium; a yeast, candida utilis; and a basidiomycete fungus, coprinus lagopus. the yeast and the fungus contain negligible endogenous cobalamin. 2. extracts of each organism catalyse c(1)-transfer from serine to homocysteine with a polyglutamate folate coenzyme. 3. the enzymes generating the methyl group of methionine from c-3 of serine have similar ... | 1972 | 4627687 |
| regulation of the formation of proteinases in bacillus megaterium. vi. some physicochemical properties of the proteinases from sporogenic and asporogenic bacillus megaterium km. | | 1972 | 4627856 |
| some unique properties of the deoxyribonucleic acid-bearing portion of the bacterial membrane. | by using the m-band technique we have shown that portions of the membranes of bacillus megaterium and escherichia coli vary in their affinity for magnesium-sarkosyl crystals and in phospholipid composition. the portion to which deoxyribonucleic acid is attached comprises as little as 4% of the total cell membrane, has a particularly high degree of affinity for magnesium-sarkosyl crystals, and is rich in phosphatidylethanolamine. the m-band fractionation does not depend on the use of lysozyme. | 1972 | 4627921 |
| properties and comparative starch-gel electrophoresis of megacins from several bacillus megaterium strains. | | 1972 | 4629134 |
| properties of thymineless strains of bacillus megaterium. | both bacillus megaterium km:t(-)r(1), a strain partially resistant to thymineless death, and strain km:t(-), the parent strain, can satisfy their thymine requirement with either thymidine, 5-methyldeoxycytidine, or 5-methyluridine. neither strain can use 5-methylcytosine, 5-hydroxymethylcytosine, 5-hydroxymethyluracil, or 5-aminouracil for this purpose. strain km:t(-)r(1) requires as little as 0.01 mm thymine for maximum growth, whereas strain km:t(-) requires 0.10 to 0.20 mm thymine. lysogenic ... | 1972 | 4629699 |
| the nutrition of bacillus megaterium and bacillus cereus. | | 1972 | 4630543 |
| [reversion of sporulating bacillus megaterium cells to growth]. | | 1972 | 4631275 |
| the effect of cytoplasmic proteins on the reaggregation of dissociated ghost membranes of bacillus megaterium km. | | 1972 | 4631734 |
| occurrence of glucosamine residues with free amino groups in cell wall peptidoglycan from bacilli as a factor responsible for resistance to lysozyme. | analysis by dinitrophenylation techniques revealed the occurrence of significant amounts of glucosamine residues with free amino groups in the peptidoglycan component of cell walls isolated from bacillus cereus, bacillus subtilis, and bacillus megaterium. a close correlation was demonstrated between the content of n-unacetylated glucosamine residues in the peptidoglycan component and the resistance of the cell walls to lysozyme. these lysozyme-resistant cell walls and peptidoglycan were converte ... | 1973 | 4632317 |
| dipicolinic acid location in intact spores of bacillus megaterium. | beta-attenuation analysis of intact spores of bacillus megaterium containing tritium-labeled dipicolinic acid has shown that dipicolinic acid is located in the spore protoplast and not in the cortex. | 1973 | 4633349 |
| location and consequences of 1,1,1-trichloro-2,2-bis(p-chlorophenyl) ethane uptake by bacillus megaterium. | no detrimental effects of 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (ddt) were observed when cells of bacillus megaterium were grown from small inocula in nutrient media containing up to 100 mug of ddt/ml. however, when the ratio of ddt to biomass of resting cells was held constant, levels of ddt as low as 1 mug/ml (0.5 mug/mg of cell dry weight) enhanced the rate of death in the population. the lethal action of ddt was both time- and dose-dependent so that higher doses required less time to ... | 1973 | 4633425 |
| demonstration of a deoxycytidine kinase activity in extracts of bacillus megaterium km. | extracts of bacillus megaterium km have been shown to possess a deoxycytidine kinase activity which functions optimally with guanosine triphosphate as the phosphoryl donor. | 1973 | 4633433 |
| binding of radioactive benzylpenicillin to sporulating bacillus cultures: chemistry and fluctuations in specific binding capacity. | the chemistry of the binding of (14)c-benzylpenicillin to sporulating cultures of bacillus megaterium and b. subtilis is similar to that in a 4-hr vegetative culture of staphylococcus aureus. unlabeled penicillins prevent the binding of (14)c-benzylpenicillin, but benzylpenicilloic acid and benzylpenilloic acid do not. bound antibiotic can be removed from cells with neutral hydroxylamine at 25 c. sporulating cultures display two intervals of enhanced binding, whereas binding to stationaryphase s ... | 1971 | 4942758 |
| the binding of chloramphenicol by ribosomes from bacillus megaterium. | | 1964 | 4953317 |
| biosynthesis of nucleic acids in bacillus megaterium. 4. roles of the 'nuclear' cytoplasmic and cytoplasmic-membrane components of the cell in the biosynthesis of ribonucleic acid. | | 1964 | 4953847 |
| [growth and sporulation of bacillus megaterium in continuous culture]. | | 1965 | 4954599 |
| [study of an l-leucyl-beta-naphthylamide hydrolase in relation to sporulation in bacillus megaterium]. | | 1965 | 4954741 |
| chloroquine: mode of action. | the drug chloroquine is bactericidal for bacillus megaterium; it inhibits dna and rna biosynthesis and produces rapid degradation of ribosomes and dissimilation of ribosomal rna. inhibition of protein synthesis is also observed, evidently as a secondary effect. inhibition of dna replication is proposed as a general mechanism of the antimicrobial action of chloroquine. | 1966 | 4955293 |
| [on the phases of spore germination of various cultures of bacillus megaterium var. phosphaticum]. | | 1965 | 4955456 |
| [evolution of the nucleic acids in bacillus megaterium]. | | 1965 | 4955543 |
| [a system of heterospecific conjugation of high incidence between staphylococcus strain 31 a and bacillus megaterium strain 207]. | | 1966 | 4955685 |
| the initial structural lesion of penicillin action in bacillus megaterium. | the effect of penicillin on the structure of bacillus megaterium cells was followed in media with and without osmotic stabilization. in peptone without osmotic support the cells showed a distortion of the normal membrane-wall relationship by 20 minutes. this appeared to be a combination of both membrane distortion and cytoplasmic leakage. lytic changes quickly followed. with osmotic support a clean-cut lesion at the transverse-septal site developed by 10 minutes' growth in penicillin. the membra ... | 1965 | 4956030 |
| site of action of certain antibacterial heterocyclic quaternary ammonium compounds. | the site of action of related mono- and bis-quinaldinium compounds was investigated in staphylococcus aureus and bacillus megaterium. the effects of these compounds on cell morphology and on protoplast formation and fragility were studied, and the distribution of c(14)-labeled quinaldinium compound in cell fractions was measured. the latter studies showed that a major part of the quaternary compound penetrates the cell, leaving a very small quantity associated with the cell wall. similar antibac ... | 1965 | 4956041 |
| on the phospholipids of bacillus megaterium. | | 1965 | 4956246 |
| inoculum-dependent division lag of bacillus cultures and its relation to an endogenous factor(s) ("schizokinen"). | lankford, c. e. (the university of texas, austin), james r. walker, james b. reeves, n. h. nabbut, b. r. byers, and r. j. jones. inoculum-dependent division lag of bacillus cultures and its relation to an endogenous factor(s) ("schizokinen"). j. bacteriol. 91:1070-1079. 1966.-when cells of bacillus megaterium, grown on brain heart infusion agar, were inoculated into a chemically defined medium, they exhibited a division lag which was an inverse function of inoculum size. the addition of filtrate ... | 1966 | 4956330 |
| response of bacillus spores to combinations of germinative compounds. | foerster, harold f. (university of texas, austin), and j. w. foster. response of bacillus spores to combinations of germinative compounds. j. bacteriol. 91:1168-1177. 1966.-spores of 21 strains of bacillus megaterium and 25 other strains representing 13 species of bacillus were produced under standardized conditions. the germination of a washed spore suspension of each strain was measured as a response to various combinations of 30 different germinative compounds. the strains were first typed wi ... | 1966 | 4956331 |
| endotrophic calcium, strontium, and barium spores of bacillus megaterium and bacillus cereus. | foerster, harold f. (the university of texas, austin), and j. w. foster. endotrophic calcium, strontium, and barium spores of bacillus megaterium and bacillus cereus. j. bacteriol. 91:1333-1345. 1966.-spores were produced by washed vegetative cells suspended in deionized water supplemented with cacl(2), srcl(2), or bacl(2). normal, refractile spores were produced in each case; a portion of the barium spores lost refractility and darkened. thin-section electron micrographs revealed no apparent an ... | 1966 | 4956334 |
| influence of exchangeable ions on germinability of bacterial spores. | rode, l. j. (the university of texas, austin), and j. w. foster. influence of exchangeable ions on germinability of bacterial spores. j. bacteriol. 91:1582-1588. 1966.-native spores of bacillus megaterium texas, and h-spores produced by titration of native spores to ph 4 with mineral acid, did not germinate in a solution of alanine and inosine unless a strong electrolyte was present. ca-spores prepared from either h-spores or native spores did germinate efficiently in the same solution without a ... | 1966 | 4956343 |
| quantitative aspects of exchangeable calcium in spores of bacillus megaterium. | rode, l. j. (the university of texas, austin), and j. w. foster. quantitative aspects of exchangeable calcium in spores of bacillus megaterium. j. bacteriol. 91:1589-1593. 1966.-more than 90% of the calcium in ca(45)-labeled native spores was released from the cells during germination. some 95% of the spore calcium was not exchangeable when ungerminated native spores were titrated to ph 4 with hno(3). ca, mg, na, si, and fe were displaced from the spores by h(+). the adsorption of ca(45) by h-sp ... | 1966 | 4956344 |
| sequence of events during bacillus megaterim spore germination. | levinson, hillel s. (u.s. army natick laboratories, natick, mass.), and mildred t. hyatt. sequence of events during bacillus megaterium spore germination. j. bacteriol. 91:1811-1818. 1966.-an integrated investigation of the sequence of events during the germination of bacillus megaterium spores produced on three different media-liver "b" (lb), synthetic, and arret and kirshbaum (a-k)-is reported. heat-activated spores were germinated in a mixture of glucose and l-alanine. for studies of dipicoli ... | 1966 | 4957022 |
| [de-acetylation of steroid acetates by means of bacillus megaterium]. | | 1965 | 4957534 |
| isolation and analysis of the protoplast membrane of bacillus megaterium. | 1. optimum conditions were found for the lysis of bacillus megaterium km by lysozyme. the age of culture, density of suspension and concentration of lysozyme affected the rate of lysis. 2. protoplast membranes were isolated by centrifugation of lysates and were exhaustively washed. 3. treatment with chloroform removed some lipid from the membranes, but about half of the total membrane lipid could be extracted only after partial acid hydrolysis. 4. the defatted membranes consisted of protein toge ... | 1966 | 4957915 |
| regulation of the formation of protease in bacillus megaterium. i. the influence of amino acids on the enzyme formation. | | 1966 | 4958280 |
| regulation of the formation of protease by bacillus megaterium. ii. formation of the enzyme by non-growing cells. | | 1966 | 4958281 |
| biosynthesis of dipicolinic acid in sporulating bacillus megaterium. | | 1966 | 4958818 |
| germination response of spores of bacillus megaterium after exposure to calcium dipicolinate at 60 degrees c. | | 1966 | 4958826 |
| partial purification and physical properties of bacillus megaterium beta-galactosidase. | | 1966 | 4959088 |
| effect of nitric oxide and hydrogen sulfide on radiation sensitivity of spores of bacillus megaterium in suspension. | | 1966 | 4959139 |
| chemical composition of the protoplast membrane of bacillus megaterium. | | 1966 | 4959361 |
| [the formation of colonies from protoplasts of bacillus megaterium]. | | 1965 | 4959630 |
| utility of sodium hypochlorite for ultrastructure study of bacterial spore integuments. | rode, l. j. (the university of texas, austin), and m. glenn williams. utility of sodium hypochlorite for ultrastructure study of bacterial spore integuments. j. bacteriol. 92:1772-1778. 1966.-spores of bacillus megaterium are partially dissolved by sodium hypochlorite. spore integuments become visible during the dissolution, and ultrastructural features may be detected. three distinct integument types are described for b. megaterium qm b1551 with the use of this technique. since a variety of mic ... | 1966 | 4959721 |
| comparative effects of 5-fluorouracil on strains of bacillus megaterium. | wachsman, j. t. (university of illinois, urbana), s. kemp, and l. hogg. comparative effects of 5-fluorouracil on strains of bacillus megaterium. j. bacteriol. 87:1011-1018. 1964.-growth of bacillus megaterium strain km is severely inhibited by 5-fluorouracil (fu). both thymidine and uridine are required to overcome this inhibition. the addition of uridine alone to a fu-inhibited culture permits good ribonucleic acid (rna) and protein synthesis for the first 2 hr, but rather poor deoxyribonucleic ... | 1964 | 4959791 |
| some differences in the action of penicillin, bacitracin, and vancomycin on bacillus megaterium. | hancock, r. (harvard medical school, boston, mass.), and p. c. fitz-james. some differences in the action of penicillin, bacitracin, and vancomycin on bacillus megaterium. j. bacteriol. 87:1044-1050. 1964.-penicillin and cycloserine do not inhibit the growth of protoplasts of bacillus megaterium, indicating that inhibition of cell-wall synthesis is the only significant process by which they inhibit growth of bacteria. in contrast, bacitracin and vancomycin inhibit growth of protoplasts and bacte ... | 1964 | 4959792 |
| thymineless death in bacillus megaterium. | wachsman, j. t. (university of illinois, urbana), s. kemp, and l. hogg. thymineless death in bacillus megaterium. j. bacteriol. 87:1079-1086. 1964.-strain km:t(-), a thymine auxotroph of bacillus megaterium strain km, rapidly loses the ability to multiply when incubated in the absence of thymine, on an otherwise sufficient medium. at 37 c, there is a lag of approximately 60 min, prior to the onset of exponential death (decrease of 1 decade per 50 min). the extent of the decrease in viable count ... | 1964 | 4959794 |
| use of thymineless death to enrich for doubly auxotrophic mutants of bacillus megaterium. | wachsman, j. t. (university of illinois, urbana), and l. hogg. use of thymineless death to enrich for doubly auxotrophic mutants of bacillus megaterium. j. bacteriol. 87:1118-1122. 1964.-when strain km:t(-), a thymine auxotroph of bacillus megaterium strain km, is allowed to undergo thymineless death on a minimal medium, the survivors are greatly enriched in polyauxotrophic mutants. cells were irradiated with ultraviolet light, grown in the presence of thymidine and a complete amino acid mixture ... | 1964 | 4959796 |
| use of 5-fluorouracil for the isolation of auxotrophic mutants of bacillus megaterium. | wachsman, j. t. (university of illinois, urbana), and l. hogg. use of 5-fluorouracil for the isolation of auxotrophic mutants of bacillus megaterium. j. bacteriol. 87:1137-1139. 1964.-the combination of 5-fluorouracil (fu) and uridine was used to selectively kill wild-type cells of bacillus megaterium km, thereby providing surviving populations greatly enriched in auxotrophic mutants. exponentially growing cells were irradiated with ultraviolet light, incubated in a basal medium containing sucro ... | 1964 | 4959798 |
| electron microscopy of bacillus megaterium undergoing isolation of its nuclear bodies. | fitz-james, philip (university of western ontario, london, canada). electron microscopy of bacillus megaterium undergoing isolation of its nucelar bodies. j. bacteriol. 87:1202-1210. 1964.-the various steps of treatment leading to the isolation of nuclear bodies were followed by thin-section electron microscopy. nuclear rearrangement (condensation then dispersion) accompanied the treatment of washed rejuvenated cells with sucrose stabilizing buffer. liberation of protoplasts with lysozyme did no ... | 1964 | 4959800 |
| iron-chelating hydroxamic acid (schizokinen) active in initiation of cell division in bacillus megaterium. | bacillus megaterium atcc 19213 secretes a cell division-initiating "schizokinen" (sk) which accumulates during its culture cycle to a concentration inversely proportional to the iron added to a sucrose-mineral salts medium. secreted sk was purified from culture filtrates as a red fe (iii) chelate, and a fraction with similar biological properties was obtained from whole cells. infrared spectra of sk, and analyses of unhydrolyzed and acid-hydrolyzed preparations indicated it to be a secondary hyd ... | 1967 | 4960152 |