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relationship of bacterial infection in urine and calculi to canine urolithiasis.in 26 dogs treated surgically for urolithiasis, bacteriological examination of the urine and the interior of calculi showed that infection was present in both materials in 14 cases. infection with phosphate calculi, present in 13 of these 14 dogs, was associated with a variety of bacteria including staphylococcus aureus, staph epidermidis, streptococcus faecalis, escherichia coli and proteus spp. in a follow-up examination of 16 dogs, organisms different from the original isolates were recovered ...1975808018
plasmid-determined resistance to erythromycin: comparison of strains of streptococcus faecalis and streptococcus pyogenes with regard to plasmid hmology and resistance inducibility.streptococcus faecalis strains ds-5 and streptococcus pyogenes strain ac-1 both have a 17 million dalton plasmid that determines resistance to erythromycin, lincomycin, and vernamycin b(alpha). the results of deoxyribonucleic acid-deoxyribonucleic acid hybridization experiments indicate that the two plasmids are about 95% homologous. it was also shown that erythromycin resistance is inducible in ac-1 and constitutive in ds-5.1975808161
editorial: bacteraemia from the bowel. 1975808245
studies of the formation of peptide cross-links in the cell wall peptidoglycan of streptococcus faecalis.a method was developed to label specifically the glycan chains of the cell wall peptidoglycan of streptococcus faecalis atcc 9790 with [14c]acetate. the formation of peptide cross-links (a) during exponential growth, (b) after valine starvation and wall thickening, and (c) during regrowth after 2 hours of valine starvation, was studied using continuous, pulse and pulse-chase labeling of the peptidoglycan with both [14c]acetate and [3h]lysine. after labeling, walls were isolated, digested with th ...1975808547
synthesis and biological activity of 10-thia-10-deaza analogs of folic acid, pteroic acid, and related compounds.the 10-thia analogs of pteroic acid, folic acid, their esters, and their 4-amino analogs were synthesized through a reaction sequence involving, as a key step, the condensation of 2-amino-3-cyano-5-chloromethylpyrazine with appropriately substituted thiols. the abilities of the products to inhibit the growth of methotrexate (mtx)-sensitive and mtx-resistant microorganisms were investigated as were their abilities to inhibit dihydrofolic acid reductase and thymidylic acid synthetase. several comp ...1975808608
alpha-methyl-l-glutamic acid uptake by high affinity dicarboxylic amino acid transport system in streptococcus faecalis.the transport of alpha-methyl-l-glutamic acid was studied in streptococcus faecalis. energey-dependent uptake against substantial concentration gradients was observed. kinetic experiments indicated that, in contrast to l-glutamic acid, only a single catalytic component (high affinity) and a diffusion controlled process participated in alpha-methyl-l-glutamic acid uptake. at concentrations up to 10 mm, alpha-methyl-glutamate transport was almost completely abolished in a mutant strain lacking a h ...1975806303
simple technique for the assay of antibiotic synergism against enterococci.a simple technique with antibiotic-impregnated disks has been developed to demonstrate the antibiotic synergism of penicillins and aminoglycosides against enterococci. the two antibiotic disks are placed on inoculated plates so that the distance between their centers is less than the sum of the two radii of their previously determined inhibitory zones. after 5 h of incubation, beta-lactamase powder is dusted onto the susceptibility plate with a sterile cotton swab. after overnight incubation, th ...1975806260
influence of environmental stress on enumeration of indicator bacteria from natural waters.the problems associated with recovery of pure cultures of escherichia coli and streptococcus faecalis from stream environments were examined utilizing membrane filter chambers. it was observed that upon exposure to the aquatic environment a significant proportion of cells lost their ability to produce colonies on a selective medium, yet retained this capability on a nutritionally rich, nonselective medium. discrepancies in colony-forming units between nonselective and selective media indicated t ...1975803815
[the association of a digestive carcinoma and subacute endocarditis. 4 cases (author's transl)].attention has recently been drawn to the association of digestive carcinoma and streptococcus bovis endocarditis. the authors studied 130 records of patients with endocarditis and an association with a digestive tumour (3 neoplasms, one villous tumour) was found in four. streptococcus bovis was demonstrated in one case, streptococcus faecalis in two and streptococcus durans in one. the association of carcinoma and subacute endocarditis would thus seem to not solely relate to digestive commensual ...1978714665
determination of the sugar sequences and the glycosidic-bond arrangements of immunogenic heteroglycans.the complete sugar sequences and glycosidic-bond arrangements have been determined by a combined analytical scheme for a diheteroglycan of d-glucose and d-galactose and a tetraheteroglycan of 6-deoxy-l-talose, l-rhamnose, d-galactose, and d-glucuronic acid. the analytical scheme included methylation analysis by g.l.c. and mass spectrometry, periodate oxidation followed by borohydride reduction and identification of the residual fragments, enzymic hydrolysis followed by characterization of the mo ...1978620418
effects of change in dietary folic acid content on pentylenetetrazol seizure threshold in rat.pentylenetetrazol (pt) eeg thresholds were measured in rats on a folic acid (fa) deficient diet, normal diet, and a diet supplemented with large amounts of folic acid. the intraperitoneal dose per kilogram body weight of pt is significantly higher in the fa deficient group and significantly lower in the fa supplemented group than in rats on a normal diet. this indicates an effect of fa metabolism on pt epilepsy. the lactobacillus casei minus streptococcus faecalis activity of the brain is decrea ...1977590221
reduction of duration and severity of megimide seizures in rats on a folic acid deficient diet.folic acid deficient diet reduces the duration and severity of intravenous megimide seizures in the rat after 6 months of diet. plasma and serum folate levels are strongly reduced after that period. the difference between lactobacillus casei and streptococcus faecalis activity in brain show a slight, but not significant, decrease after 12 months of diet.1977590220
effect of cerulenin on streptococcus faecalis macromolecular synthesis and cell division.the antibiotic cerulenin has been used to study macromolecular synthesis and cell division in streptococcus faecalis. the data suggest that lipid and lipoteichoic acid synthesis as well as cell number increase are affected prior to any observable effects on overall mass increase or dna, rna, protein, or peptidoglycan synthesis. treatment with cerulenin of cultures growing at various rates and analysis of the subsequent cell divisions indicate that the antibiotic may block a cell cycle event that ...1978415039
structural arrangement of polymers within the wall of streptococcus faecalis.the structure of the cell wall of streptococcus faecalis was studied in thin sections and freeze fractures of whole cells and partially purified wall fractions. also, the structures of wall preparations treated with hot trichloroacetic acid to remove non-peptidoglycan wall polymers were compared with wall preparations that possess a full complement of accessory polymers. the appearance of the wall varied with the degree of hydration of preparations and physical removal of the cell membrane from ...1978412837
studies of introital colonization in women with recurrent urinary infections. viii. the role of bacterial interference.the non-pathogenic aerobic bacteria in 97 quantitative introital cultures from 11 women with documented recurrent bacteriuria were compared statistically to those in 100 quantitative introital cultures from 10 control women with no history of bacteriuria. the colonization incidence of the non-pathogenic organisms was not statistically different for the 2 groups, except for gamma hemolytic streptococcus (p equals 0.002). no strongly significant correlations were demonstrated between the colonizat ...1977408518
bacteriological studies in a patient with antibiotic-associated pseudomembranous colitis.detailed aerobic and anaerobic bacteriological studies on the operative specimen from a patient with antibiotic-associated pseudomembranous colitis revealed approximately 10(11) facultatively anaerobic bacteria, but less than 10(5) obligate anaerobes per g of involved colonic tissue. fourteen isolates of escherichia coli, three isolates of pseudomonas aeruginosa, and two isolates of streptococcus fecalis were identified. the majority of the organisms were resistant to most of the antibiotics tha ...1977405269
sorption of streptococcus faecium to glass.a method has been developed by which to study the sorption of streptococcus faecium to soda-lime cover glasses. conditions were chosen to minimize the influence on sorption of bacterial polymer production, passive sorption being studied rather than attachment mediated by metabolic activities. sorption of s. faecium increased with increasing temperature (to 50 degrees c), time, and cell concentration, but equilibrium apparently was not reached even after incubation for 8 hours or at a cell concen ...1977402789
selective electrode for dibenzyl dimethyl ammonium cation as indicator of the membrane potential in biological systems.the electrode sensitive to dibenzyl dimethyl ammonium (dda+), which is considered to be an indicator of the membrane potential, was constructed by using tetraphenyl borone (tpb-) embedded in dichloroethane. rapid and nernstian responses were exhibited against dda+ solutions ranging between 10(-2) and 3 - 10(-6) m in concentration. high selectivity for dda+ was observed in the presence of various inorganis salts, adp, atp, oxidizable substrates and sugars. the electrode developed here was used to ...1977402152
collaborative study of turbidimetric microbiological assay of menensin in chicken rations. 1977401805
superoxide dismutase and oxygen metabolism in streptococcus faecalis and comparisons with other organisms.streptococcus faecalis contains a single superoxide dismutase that has been purified to homogeneity with a 55% yield. this enzyme has a molecular weight of 45,000 and is composed of two subunits of equal size. it contains 1.3 atoms of manganese per molecule. its amino acid composition was determined and is compared with that for the superoxide dismutases from escherichia coli, streptococcus mutans, and mycobacterium lepraemurium. when used as an antigen in rabbits, the s. faecalis enzyme elicite ...1978206536
distribution of cytochrome-like respiration in streptococci.the electron transport systems of 134 strains of streptococci were studied after aerobic growth on glucose in the presence of haematin, by examining the inhibition of electron transport as well as the cellular site of nadh oxidation. each strain was placed into one of three possible groups: cytochrome-like nadh oxidase; flavin-like nadh oxidase; or no nadh oxidase. most (88%) of the strains of streptococcus faecalis and its variants liquefaciens and zymogenes and a few strains of s. lactis and i ...1976180234
[osmotic activity and ionic permeability of membrane vesicles from streptococcus faecalis and micrococcus lysodeikticus cells].membrane fractions containing osmotically active vesicles with sufficiently low membrane permeability for k+, na+ and cl- ions typical for the intact cell membrane were isolated from the cells of the glycolyzing bacterium streptococcus faecalis. in their osmotic properties and ionic permeability the membrane fractions of s. faecalis were found similar to those of the respiring bacterium micrococcus lysodeikticus, which are capable of the energy-dependent potassium transport. it may be thus assum ...1977145881
studies of substructure and tightly bound nucleotide in bacterial membrane atpase.highly purified preparations of streptococcus faecalis atpase contain a similar but inactive protein detected by prolonged polyacrylamide gel electrophoresis. the inactive protein appears to arise by proteolytic cleavage of the major subunits in the enzyme. by use of a new technique, subunit analysis in sds gels was performed on the enzyme band and the inactive protein band excised from a polyacrylamide gel after electrophoresis. the results indicated that the atpase has the composition alpha3be ...1975127088
characterization of plasmids determining hemolysin and bacteriocin production in streptococcus faecalis 5952.two plasmids designated pob1 and pob2 were isolated from streptococcus faecalis strain 5952 and found to have molecular weights of approximately 46 x 10(6) and 28 x 10(6), respectively. pob1 was found to determine hemolytic activity and was transmissible, whereas pob2 appeared to determine a bacteriocin that is specifically inhibitory to s. faecalis strains harboring the 26-megadalton plasmid pam539.1977122514
analysis of plasmid deoxyribonucleic acid in a cariogenic strain of streptococcus faecalis: an approach to identifying genetic determinants on cryptic plasmids.streptococcus faecalis strains nd539 and og1 have been previously shown to be cariogenic in gnotobiotic animals. deoxyribonucleic acid analyses have revealed the presence of a single 26-megadalton plasmid designated pam539 in the former strain, whereas the latter strain was found to be plasmid-free. by gene transfer experiments, it was possible to construct isogenic pairs of strains that differed only with regard to the presence or absence of pam539. comparative studies of isogenic pairs showed ...1977122512
trout mortalities as a result of streptococcus infection.excessive mortalities were experienced in the bigger rainbow trout (salmo gairdneri) at an efficiently managed trout farm. all tests for known toxins in the feed and water proved to be negative. a faecal streptococcus which belongs to the lancefield group d but which could not be identified as belonging to any of the recognized species within this group, was isolated from the spleens, livers and kidneys of affected fish. pathogenicity studies with this organism proved it to be highly fatal to tr ...1979121769
effects of cerulenin on antibiotic-induced lysis of streptococcus faecalis (s. faecium).addition of the antibiotic cerulenin to cultures lowered the minimal effective concentration of penicillin g or methicillin required to produce bacterial lysis and killing. this effect was most pronounced at subinhibitory antibiotic concentrations. cerulenin had no significant effects on lysis or killing induced in the presence of d-cycloserine, fosfomycin, bacitracin, or vancomycin.1979119485
kinetic studies on enzymatic acetylation of chloramphenicol in streptococcus faecalis.the kinetics of chloramphenicol (cp) acetylation by cp acetyltransferase from streptococcus faecalis was studied. cp was shown to be acetylated enzymatically to its 3-o-acetyl derivative (3-accp) in the presence of acetyl coenzyme a, after which 3-accp was converted nonenzymatically to its 1-o-acetyl isomer, 1-o-acetyl cp (1-accp). at equilibrium, the 1-accp and 3-accp were present in a 1:4 ratio. subsequently the diacetylated product, 1,3-o-o-diacetyl cp [1,3-(ac)2cp], was enzymatically produce ...1979119483
high-level, plasmid-borne resistance to gentamicin in streptococcus faecalis subsp. zymogenes.each of three isolates of streptococcus faecalis subsp. zymogenes harbored three r plasmids and a hemolysin-bacteriocin plasmid. the plasmids carried by one of these strains were physically characterized after their conjugative transfer. in each strain one of the plasmids carried genetic markers for resistance to gentamicin, kanamycin, sisomicin, netilmicin, and tobramycin.1979118707
synthesis and biological activity of 5-fluoro-4'-thiouridine and some related nucleosides.the synthesis of a series of 4'-thio-5-halogenopyrimidine nucleosides, including the 5-fluoro, chloro, bromo and iodo derivatives, has been carried out by condensation of the 2,4-bis-o-trimethylsilyl derivatives of the corresponding pyrimidine bases with the protected 4-thio-d-ribofuranosyl chloride. among these, the alpha and beta anomers of 4'-thio-5-fluorouridine inhibited the growth of leukemia l1210 cells at concentrations of 4 x 10(-7) and 2 x 10(-7) m, respectively, and that of s. faecium ...1975808609
methotrexate analogs. 6. replacement of glutamic acid by various amino acid esters and amines.a series of methotrexate (mtx) analogs was prepared in which the glutamic acid moiety is replaced by various amino acid esters and amines. the synthetic method consisted of the reaction of 4-amino-4-deoxy-n10-methylpteroic acid with various reagents to form intermediate mixed anhydrides, which then reacted with amino acid esters or amines to give the mtx analogs. these compounds were tested for antibacterial activity against streptococcus faecium and for antitumor activity against l1210 leukemia ...1975808614
[bacteriological detection of asymptomatic urinary tract infections]. 1975808673
detection of bacteriuria in pediatric outpatients. a new culture device.urine samples from 1,003 pediatric patients were examined for bacteriuria with a culture-nitrite strip test, along with microscopic assessment of pyruia and conventional chemical analysis. the latter two methods proved of little value in reliably detecting or excluding substantial bacteriuria. an overall prevalence of 5.2% was shown. using microstix reagent strips, 92 urine samples with substantial colony counts were detected. subculture from the strips disclosed that 52 of these involved single ...1975808961
an assessment of antibiotics for the treatment of pyogenic dental infections.tests made with a series of antibiotics against a number of different organisms indicate that if clinical evidence suggests a streptococcal infection, penicillin should be used otherwise erythromycin is preferred.1975809025
effect of hyperbaric helium on vitamin uptake and utilization by micro-organisms.growth responses under hyperbaric helium of various procaryotic micro-organisms to graded levels of six water-soluble vitamins were measured. vitamins included: thiamin, riboflavin, niacin, pantothenic acid, biotin, and folic acid (folacin). growth response of each organism under hyperbaric helium was compared with that under 80% he-20% o2 and other he-o2 gas mixtures at atmospheric pressure, and with response in air at atmospheric pressure. for five of the six vitamins, no differences in respon ...1975809032
[effect of the combination of 5-methylquinolin-8-ol, 7-bromo-5-methylquinolin-8-ol and 5-methylquinolin-8-ol-dodecylsulfate on the intestinal flora of healthy subjects].the influence of a combination of 5-methyl-quinolin-8-ol, 7-bromo-5-methyl-quinolin-8-ol with 5-methyl-quinolin-8-ol-dodecylsulfate on the composition of intestinal bacteria of healthy adults has been examined. the composition of normal anaerobic and aerobic intestinal bacteria practically showed no change.1975809045
the structure of dihydrofolate reductase. identification of methionine residues carboxymethylated by iodoacetate with loss of catalytic activity.dihydrofolate reductase from the amethopterin-resistant mutant (strain a) of streptococcus faecium var. durans was reacted with iodo[14c]acetate according to three procedures; (a) in the absence of an inhibitor, (b) in the presence of aminopterin, and (c) in absence of inhibitor, but after treatment with unlabeled iodoacetate in presence of aminopterin. the first and last procedures resulted in the loss of approximately 90% of the catalytic activity, whereas in the presence of aminopterin essent ...1975809271
antibacterial activity of human cervical mucus.the antimicrobial property of human uterine cervical mucus was tested in three groups of women. healthy women, using no contraception, women using an intrauterine device and women receiving hormonal treatment for contraception. cervical mucus was taken on the 10th, 14th, 18th and 22nd day of the menstrual cycle. cervical mucus had a strong inhibitory effect on the growth of micrococcus lysodeicticus in all three groups. the strength of the inhibitory effect on the other microorganisms were in t ...1975809320
osteomyelitis caused by streptococcus faecalis (report of an unusual case). 1975809352
evidence for the synthesis of soluble peptidoglycan fragments by protoplasts of streptococcus faecalis.growing protoplasts of streptococcus faecalis 9790 were found to synthesize and excrete soluble peptidoglycan fragments. the presence of soluble peptidoglycan derivatives in culture supernatants was determined by (i) incorporation of three different radioactively labeled precursors (l-lysine, d-alanine, and acetate) into products which, after hen egg-white lysozyme hydrolysis, had the same kd values on gel filtration as muramidase hydrolysis products of isolated walls; (ii) inhibition of net syn ...1975809417
characterization of the presumed peptide cross-links in the soluble peptidoglycan fragments synthesized by protoplasts of streptococcus faecalis.protoplasts of streptococcus faecalis atcc 9790 were produced with the aid of lysozyme, and the ability of these bodies to synthesize soluble, peptide cross-linked peptidoglycan (pg) fragments was examined. lysozyme digests of pg isolated using gel filtration from the supernatant medium of protoplasts grown in the presence of [14c]acetate and l-[3h]lysine contained small amounts of pg having kd expected for peptide cross-linked dimers and trimers. addition of benzyl penicillin (300 mug/ml) to gr ...1975809418
synthesis of peptidoglycan in the form of soluble glycan chains by growing protoplasts (autoplasts) of streptococcus faecalis.protoplasts (autoplasts) of streptococcus faecalis were produced by the action of native autolytic n-acetylmuramidase in the absence of added peptidoglycan hydrolases and were grown in osmotically stabilized medium containing l-[3h]lysine and d-[14c]alanine. to reduce the level of muralytic hydrolysis of glycan chains during growth, heat-inactivated cell walls were added to the medium to bind autolytic enzyme, and tetracycline (1 mug/ml) was added to inhibit further enzyme synthesis. under these ...1975809419
nucleic acid related compounds. 17.3-deazuridine. stannous chloride catalysis of cis-diol vs. phenolic base methylation with diazomethane.treatment of a methanolic solution of 4-hydroxy-1-beta-d-ribofuranosyl-2-pyridinone (3-deazauridine, 1) with diazomethane gave 2-methoxy-1-beta-d-ribofuranosyl-4-pyridinone (2) and 4-methoxy-1-beta-d-ribofuranosyl-2-pyridinone (3a) in an approximate ratio of 1:2. analogous treatment of 1 with diazomethane in the presence of stannous chloride dihydrate gave eight detected products including 2, 2-methoxy-1-(2-o-methyl-beta-d-ribofuranosyl)-4-phridinone (4), 2-methoxy-1-(3-o-methyl-beta-d-ribofuran ...1975809580
'single-stranded' dna from phix174 and m13 is cleaved by certain restriction endonucleases. 1975809717
[change in the accumulation of dibenzyldimethylammonium by irradiated cells of streptococcus under the effects of modifiers of radiation injury]. 1975810185
relationship between various bacterial populations and coprostanol and cholesterol.a year-long study was conducted at three different locations in canada to evaluate the relationship between fecal sterols and bacterial populations. a statistical model was constructed to study the following: (i) seasonal variations in the sterols and bacterial parameters; (ii) the differences between morning and afternoon samples; (iii) the relationship between the two sterols (coprostanol and cholesterol) as well as their relationship to the bacterial parameters; and (iv) the geographical diff ...1975810236
survival of salmonella and other indicator microorganisms.survival times for enteric bacteria and fecal streptococci were investigated using filter-sterilized water from a northern illinois river and temperatures of 0 degrees, 5 degrees, 10 degrees, and 20 degrees c. survival of salmonella typhimurium was essentially the same as escherichia coli over normal seasonal temperatures. fecal streptococci generally exhibited classic survival patterns.1975810237
streptococcal infections--updated.streptococci are amont the most common bacterial pathogens physicians encounter in practice. infections with streptococci continue to occur with significant frequency despite the general sensitivity of these organisms to a variety of widely used antibiotics. in newborn infants and other special patient groups, streptococci may produce fulminant and fatal sepsis (table 1). in normal children and adults, infections usually are short term and often mild or unrecognized but with the possibility of r ...1975810336
the antibacterial and pharmacological activity of oxolinic acid (prodoxol). 1975810473
transmissible toxin (hemolysin) plasmid in streptococcus faecalis and its mobilization of a noninfectious drug resistance plasmid.streptococcus faecalis strain ds-5 has been shown previously to contain three plasmids, pamalpha1, pambeta1, and pamgamma1. mixed incubation of ds-5 with strain jh2-2, a plasmid-free s. faecalis recipient, results in the transfer of pamalpha1 (which determines resistance to tetracycline) and/or pamgamma1. analyses of recipients carrying various combinations of these plasmids have revealed the pamgamma1 codes for toxin (hemolysin) production and two bacteriocin activities. jh2-2 strains carrying ...1975810482
phage typing scheme for group d streptococci isolated from human urogenital tract.streptococci of lancefield group d were isolated from 52.2% of pharyngeal, 55.5% of urethral, 56.6% of endocervical, and 75.4% of ano-rectal specimens. seventeen phages isolated from sewage and urogenital specimens were selected for phage typing. four of these lysed only the strains of streptococcus faecalis and its variety liquefaciens. another six phages lysed only the strains of streptococcus faecium and its variety durans. with the help of seven bacteriophages, 346 of 384 (90.1%) strains of ...1975810495
[effects of formula diet with varying carbohydrate proportion on gut microflora in man].the effects of conventional food and of formula diet on gut microflora were tested in six healthy persons. in comparison with conventional food, the total gut microflora concentrations slightly increased during formula diet with oligosaccharides. during the periods of formula diet rich in sucrose of maltose, total flora concentrations declined. these changes of total gut microflora were especially caused by the increase and decrease of the enterococci and enterobacteria, the bacteroides showing ...1975810751
streptococcus faecalis and streptococcus faecium in infected dental root canals at filling and their susceptibility to azidocillin and some comparable antibiotics.the study was performed on 612 routine cultures of material obtained from root canals of teeth at the time of filling (r-cultures) by students at the department of endodontics during a continuous period of one year. twenty-nine isolates from 27 (29.3%) of the 92 positive cultures filled the criteria of enterococci (sherman, 1937) and had demonstrable group d-antigen. with a set of tests these isolates were identified as follows: strep. faecalis subsp. faecalis (10), strep. faecalis subsp. zymoge ...1975810753
the effect of neutralizing agents on aqueous parachlorophenol.the effect of possible neutralizing agents on aqueous parachlorophenol were studied. results indicated that saline, edtac, dentin, saliva, prolonged light exposure, and thermal extremes do not appreciably affect the antibacterial properties of aqueous parachlorophenol. the presence of blood and necrotic tissue demonstrated a marked inhibitory effect.1975810757
experimental intestinal granulomas. 1975810807
[study of enterococci in milk].studied were a total of 270 samples of raw and pasteurized milk taken during the various technologic states of pasteurized mild produced for consumption at the state economic enterprise "serdica", establishing the enterococcus and coli titers. it was found that the enterococcus titer of the raw milk varies from 10(-3) up to 10(-6), and the titer of the pasteurized milk at the enterprise and upon delivery through its commercial channels is from 1 up to 10(-3). the enterococcus titer of milk that ...1975810940
glomerular and vascular lesions in chronic atrophic pyelonephritis and reflux nephropathy. 1975811093
inhibition of microorganisms by pyrimidine nucleosides. 1975811152
evaluation of a most-probable-number technique for the enumeration of pseudomonas aeruginosa.a most-probable-number (mpn) technique was evaluated for detecting and enumerating pseudomonas aeruginosa in water and wastewater. both the presumptive and confirmatory media, as described in the 13th edition of standard methods for the examination of water and wastewater, as well as modifications of these media were included in evaluations. various samples of water were tested, namely chlorinated tap water, creek water, and influent to a wastewater treatment plant. modified media repeatedly gav ...1975811166
distribution and significance of fecal indicator organisms in the upper chesapeake bay.total viable aerobic, heterotrophic bacteria, total coliforms, fecal coliforms, and fecal streptococci were enumerated in samples collected at five stations located in the upper chesapeake bay, december 1973 through december 1974. significant levels of pollution indicator organisms were detected at all of the stations sampled. highest counts were observed in samples collected at the confluence of the susquehanna river and the chesapeake bay. the indicator organisms examined were observed to be q ...1975811167
prophylactic interparietal povidone-iodine in abdominal surgery.this study sought to determine in a district general hospital (a) the frequency and nature of bacterial contamination at operation, (b) the incidence of consequent infection and (c) the prophylactic effect, if any, of interparietal povidone-iodine against postoperative wound infection. bacterial contamination was shown in 49 per cent of all the abdominal surgical wounds at the end of operation. from 61 per cent of the subsequently infected wounds, organisms identified as contaminants at operatio ...1975811302
morphology and viability of large bodies of streptococcal l-forms.a procedure is described for the massive formation and isolation of large bodies of group a and d streptococcal l-forms. up to 90% individual bodies of 20 to 100 mum in diameter can be produced in pour plates of nutrient gelatin and subsequently harvested by simple micromanipulation. the growth of these giant bodies was followed by light microscopy and their ultrastructure and internal architecture was examined by transmission and scanning electron microscopy. large bodies had a honeycomb struct ...1975811562
selective adhesion of microorganisms to the ductular epithelium of the bovine mammary gland.streptococcus agalactiae, streptococcus faecalis, staphylococcus aureus, escherichia coli, and corynebacterium bovis were examined for their ability to adhere to the ductular epithelial cells of the bovine udder. s. agalactiae and s. aureus adhered readily and in large numbers, whereas the other organisms adhered poorly or not at all. the organisms showing the ability to adhere are those which frequently cause mastitis. these data suggest that selective adherence to the ductular epithelium may b ...1975811566
adherence of bacteria to heart valves in vitro.the abilities of 14 strains of aerobic gram-positive cocci and gram-negative bacilli to adhere in vitro to human or canine aortic valve leaflets were compared. 2-mm sections of excised valve leaflets were obtained by punch biopsy and were incubated under standardized conditions in suspensions of bacteria. valve sections were subsequently washed and homogenized, and quantitative techniques were used to determine the proportions of bacteria from the initial suspensions that had adhered to the valv ...1975811687
streptococcus faecalis chain disruption. 1975811762
quinazolines as inhibitors of dihydrofolate reductase. 3. analogs of pteroic and isopteroic acids.a series of 19 quinazoline analogs of pteroic and isopteroic acid was prepared with particular emphasis being placed upon carboxylic acid esters. each compound was evaluated as an inhibitor of the dihydrofolate reductases from rat liver as well as from streptococcus faecium. several of the more potent inhibitors were found to be inactive against l1210 leukemia in mice at low dose levels and were lethal to mice at 100 mg/kg. six compounds were also evaluated for antimalarial activity against plas ...1975811798
3-acyloxymethyl-7-(2-thienylacetamido)-3-cephem-4-carboxylic acids. an improved synthesis and biological properties. 1975811800
studies on the establishment of multi-drug-resistant strain bio-4r of streptococcus faecalis in the intestinal tract of germ-free mice. bacterial interaction and effect of antibiotics.germ-free icr mice were mono- or dicontaminated with a multi-drug-resistant strain bio-4r of streptococcus faecalis (bio-4r) and escherichia coli 026 : k60 (e. coli) and administered aminobenzyl penicillin (abpc). bio-4r was established in the intestinal tract at a level of 10(8) viable cells per gram of stool on the fourth day following oral inoculation and the bio-4r population was stably maintained thereafter. the drug resistance of bio-4r remained unchanged in the intestinal tract of gnotobi ...1975811833
cellular immunity in experimental pyelonephritis. 1975811919
[comparative study of some quaternary ammoniums neutralizing agents. influence of the nature of the bacterial strains used].the neutralising capacity of the anti-bacterial activity of a quaternary ammonium salt, depends on the nature of the bacterial strains used. consequently, the choice of a neutralising agent of the bactericidal activity can only be made following a preliminary study on several bacterial species. out of five products which we tested, we found the tween-lecithin fresh egg mixture alone had satisfactory activity.1975812041
ecological studies of the microbial flora of the female lower genital tract. 1975812101
[studies on enterocinogens of enterococci present in food products and of their role in the mechanism of antagonisms]. 1975812169
[comparative studies on the disinfection effect of bromine and chlorine in swimming pools and model tests]. 1975812178
[microflora and immunity in surgical patients. i. some microfloral indices in the pre- and postoperative periods].surgical treatment can lead to the appearance of a purulent staphylococcus infection of endogenous etiology. endogenous infection originates as a result of the patient's diminished resistance, indicated by an increase in the number of pathogenic staphylococci, the appearance of representatives of the intestinal flora in the oral cavity and on the surface of the skin. no iogenous purulent infection.1975812300
[biocenotic dynamics of liquid sewage in the process of its biological purification at aeration stations].a study was made of biological purification of sewage at the aeration stations on the quantitative composition of the main indicator microbes--of bacteria of the coliform group and of the fecal coliform bacilli, enterococci, proteus, and also pathogenic enterobacteria. there was found a difference in the behaviour of different species of proteus, i.e. reduction in the process of purification in the numbers of pr. mirabilis, and a sharp elevation of pr. morganii content. there was noted an insign ...1975812301
seasonal occurrence and distribution of microbial indicators and pathogens in the rhode river of chesapeake bay.the seasonal incidence and occurrence of indicator organisms and pathogens were studied at four sites in the rhode river, a subestuary of chesapeake bay. the highest frequency of occurrence of total and fecal coliforms and fecal streptococci was in muddy creek, a marsh area receiving pasture land runoff. second highest frequency of occurrence of these bacteria was in cadle creek, a populated area. lowest measurements of these parameters were obtained at stations in the central portion of the rho ...1975812423
role of amino acids on the growth and lipase production of streptococcus faecalis.a study was conducted on the requirement of amino acids for the growth of s. faecalis and its lipase production. arginine, glutamic acid, histidine, leucine, isoleucine, methionine, threonine, tryptophane and valine were found to be essential, with alanine, glycine, lysine and serine as stimulatory and aspartic acid, cystine, phenylalanine, proline and tyrosine as non-essential for both growth and lipase synthesis.1975812727
studies of balanced and unblaanced growth of streptococcus mutans. 1976812891
chronic granulomatous disease in an adult male: a proposed x-linked defect.a 25-year old patient with chronic granulomatous disease of somewhat unusual history is described. the diagnosis of cgd was based on increased susceptibility to infection, granulomatous appearance of tissues, and diminished bactericidal and metabolic response of leukocytes during phagocytosis: the clinical and cellular features considered phenotypic of cgd. a 16-year-old female sibling had bactericidal and metabolic abnormalities of leukocyte function similar to those of the patient's leukocytes ...1976812972
synthesis of inhibitors of bacterial cell wall biogenesis. analogs of d-alanyl-d-alanine. 1976812991
chemistry and antibacterial activity of nitrobenzofurans.thirteen 2-methylbenzofurans were synthesized and their antibacterial activity was investigated. 2-methyl-3-nitrobenzofuran and analogs containing 7-no2, 5-no2, 7-br, 7-conh2, and 7-cf3 substituents are bacteriostatic. the spectrum of activity of these compounds is similar to nitrofurazone; however, a strain of e. coli br which has increased resistance to nitrofurazone did not show increased resistance to 3,7-dinitro-2-methylbenzofuran (1). the 3-nitro-2-methylbenzofurans are labile in solution ...1976812994
the faecal flora in ulcerative colitis.differential counts of the faecal flora of patients with ulcerative colitis showed a dysbiotic flora with a 100-fold increase of group-d streptococci and a reduction of bifidobacteria in comparison with the stable eubiotic flora of healthy subjects. the increase in number in group-d streptococci was accompanied by an increase in variety. about four different varieties of enterococci were found in faeces from patients compared with one or two in samples from healthy subjects. the strains isolated ...1975812997
[the antifolic activity of several derivatives of 2,4,6-triaminopyrimidine and 2,6-diaminopurine in a microbiologic test system]. 1975813381
severe disabling polyarthritis associated with bacterial endocarditis. 1975813398
infection following total hip replacement in a general hospital without special orthopaedic facilities.infection following total hip replacement is a serious complication for it is frequently impossible to resolve without removal of the prosthesis. we have reviewed 321 total hip replacements undertaken in a general hospital without special orthopaedic theatres. there were 17 deep infections, nine early and eight late. athough the diagnosis of early infection is usually not difficult, the differentiation between late infections, mechanical failure and metal sensitivity may be a problem. this paper ...1975813492
on the mode of action of 5-diazouracil on bacterial cell division.cell division by strains of escherichia coli and salmonella typhimurium is inhibited by 5-diazouracil (5-du). division recovers in the presence of the inhibitor after a period which is temperature-dependent. recovery is probably due to breakdown of 5-du and the rate of this breakdown is apparently increased at alkaline ph. growth with 5-du caused only a slight reduction in the rate of murein synthesis and no alteration in the properties or composition of membranes of s. typhimurium. the agent ca ...1976813834
activation of lipolytic activity of streptococcus faecalis by nitrosoguanidine. 1976814069
microbial synergy in experimental intra-abdominal abscess.intra-abdominal sepsis was studied in wistar rats by using four microbial species: escherichia coli, enterococci, bacteroides fragilis, and fusobacterium varium. these organisms were implanted into the peritoneal cavity singly and in all possible dual combinations. results were evaluated by mortality rates and the incidence of intra-abdominal abscesses on autopsy following sacrifice after 7 days. mortality was restricted to recipients of e. coli, thus implicating coliforms in the acute lethality ...1976814099
selective adherence as a determinant of the host tropisms of certain indigenous and pathogenic bacteria.the relationship between the selective abilities of bacteria to adhere and their predilections for colonizing different mammalian hosts was investigated by using bacteria indigenous to the tongue dorsum of humans and rats as models. streptococcus salivarius and s. sanguis averaged 22.6 and 2.8%, respectively, of the cultivable bacteria recovered from swab samples of the tonges of five humans, but these organisms were not indigenous on the tongues of rats (charles river strain). s. faecalis and s ...1976814100
bacterial endocarditis. echocardiographic and clinical evaluation during therapy.in two patients with bacterial endocarditis and apparent vegetations, the echocardiographic findings included thickening but normal excursion of the mitral leaflet and abnormal shaggy echoes superimposed on the mitral leaflet echogram. both patients had had endocarditis several weeks before the study was performed. in both patients the abnormal echoes disappeared after antibiotic therapy. whether or not the echocardiographic findings are specific to bacterial endocarditis must be determined by f ...1976814136
antimicrobial efficacy of reduced concentrations of parachlorophenol in extracted teeth. 1975814185
racemic diastereoisomers of 1-amino-2-hydroxycyclopentanecarboxylic acid.the synthesis and characterization of the two diastereoisomeric forms of 1-amino-2-hydroxycyclopentanecarboxylic acid have been accomplished. a previously reported synthesis produced a racemic mixture of the threonine analog trans-2-hydroxy-1-aminocyclopentanecarboxylic acid (trans with respect to the hydroxy and carboxyl group). the alternate allothreonine analog was produced by conversion of cyclopentene oxide to trans-2-methoxycyclopentanol, followed by oxidation to 2-methoxycyclopentanone an ...1976814238
products from furans. 1. sunthesis and anticoccidial and antimicrobial activity of 5-amino-5,6-dihydro-6-methoxy-2-methyl-2-(4'-biphenylyl)-2h-pyran-3(4h)-ones and related compounds.a michael type addition of an amine to 6-methoxy-2-methyl-2-(4'-biphenylyl)-2h-pyran-3(6h)-one (1) dissolved in ether, benzene, or thf gave 5-amino derivatives of 5,6-dihydro-6-methoxy-2-methyl-2-(4'-biphenylyl)-2h-pyran-3(4h)-one (2). these by subsequent reduction with lialh4 were converted to 5-amino derivatives of 6-methoxy-2-methyl-2-(4'-biphenylyl)tetrahydro-2h-pyran-3-ol (3). both isomers a and b of 1 (in regard to the methoxy group at c6) were used for the synthesis of 2 and 3. the in vit ...1976814239
[inoculability and bacteriocinogenic activity of enterococci]. 1975814387
microstix-a reagent strip for urine culture.microstix is a simple and convenient reagent strip for obtaining a semi-quantitative bacterial count on urine specimens. this method correctly predicted over 10(5) gram-negative organisms per ml in 84 percent of samples and over 10(5) gram-positive organisms per ml in 72 percent. urine specimens containing 10(4) per ml or more of any organism should be recultured using fully quantitative methods. if this criterion had been used 89 percent of urine samples with over 10(5) organisms per ml would h ...1975814509
a sensitive radioactive method for determination of glutamine. 1975814840
the effect of cooling and warming rates on the survival of a variety of bacteria.cooling and warming rates affect bacterial survival profoundly with all bacteria tested (azotobacter chroococcum, klebsiella aerogenes, salmonella typhimurium, pseudomonas aeruginosa, streptococcus faecalis) behaving similarly. most bacteria were sensitive to salt on freezing and thawing. viabilities of population frozen and thawed in saline were always less (by at least 20%) than comparable ones frozen in water alone. under these conditions, s. faecalis was resistant to the presence of sodium c ...1976814981
chemical modification of peptide antibiotics : part iii. biological activities of some ethanolamine and tryptophan derivatives and of some polypeptides containing tryptophan. 1975815158
importance of streptococci as pathogens in the urinary tract.streptococcus faecalis, while the most common streptococcal serotype causing urinary tract infection, is not the only one. lancefield group a, b, c and g serotypes are capable of invading the urinary tract. this finding is not generally appreciated. the reason for this anomaly is seen in the failure of most investigators to utilise a definitive procedure to group streptococcal isolates from urine. streptococci are responsible for 8.5 percent of urinary tract infections in this hospital.1975815857
accelerated healing in infected wounds.the effect of a standardized infection of pure and mixed cultures of gram-negative bacteria and a gram-positive coccus was studied in laparotomy wounds of rats. the infections were significantly stronger than in the control group wounds in 14 of 15 comparisons. this increased strength could not be correlated with an increased wound content of collagen. results of histologic studies showed a vigorous inflammatory response which probably was responsible for the increased strength. there was a sugg ...1976816018
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