| ion extrusion systems in bacteria. | | 1985 | 2418727 |
| adherence of streptococcus sanguis to hydroxyapatite coated with lysozyme and lysozyme-supplemented saliva. | the adherence of [3h]thymidine-labeled streptococcus sanguis strains to bare hydroxyapatite and to hydroxyapatite coated with a range of concentrations of lysozyme, poly-l-lysine, poly-l-glutamic acid, whole saliva supernatant, and combinations of some of the above was studied. adherence of several strains of s. sanguis to bare hydroxyapatite and saliva-coated hydroxyapatite was compared. saliva present as a pellicle on the hydroxyapatite inhibited adherence of some strains (903, m-5, 73x11) and ... | 1986 | 2419251 |
| strains of streptococcus mutans and streptococcus sobrinus attach to different pellicle receptors. | we compared the levels of adsorption of streptococcus mutans jbp and streptococcus sobrinus 6715 to experimental pellicles formed from unsupplemented and glucosyltransferase (gtf)-supplemented saliva. pellicles formed on hydroxyapatite beads from gtf or from saliva-gtf mixtures possessed detectable gtf activity. low levels of gtf activity were also detected in clarified whole human saliva, but not in samples of submandibular saliva. the adsorptive behavior of s. mutans jbp to pellicles formed fr ... | 1986 | 2422126 |
| comparative adhesion of seven species of streptococci isolated from the blood of patients with sub-acute bacterial endocarditis to fibrin-platelet clots in vitro. | the adhesion to fibrin-platelet clots in vitro of 21 strains of streptococci isolated from the blood of patients with sub-acute bacterial endocarditis (sabe) was measured. the species, in order of greatest adhesion, were streptococcus faecalis, streptococcus mutans, streptococcus milleri, streptococcus sanguis, dextran-positive streptococcus mitior, dextran-negative streptococcus mitior and streptococcus salivarius. individual strains within species, however, cannot be assumed to be representati ... | 1986 | 2422150 |
| monoclonal antibodies against isotypic and isoallotypic determinants of human iga1 and iga2: fine specificities and binding properties. | we have analysed and compared the fine specificity and behavior in various immunoassays of 10 mouse monoclonal antibodies, from three independent laboratories, directed against iga1, iga2 or non-iga2m(2). the following observations were made. (1) although all of the monoclonal antibodies were specific for a particular iga subclass or isoallotype in a radioimmunoassay, three of them were not specific when tested in indirect immunofluorescence on plasma cells derived from pokeweed-activated periph ... | 1986 | 2425248 |
| cell-surface proteins of streptococcus sanguis associated with cell hydrophobicity and coaggregation properties. | incubating cells of streptococcus sanguis with sodium lauroyl sarcosinate, under conditions that did not cause lysis, solubilized material comprising 5-8% of the cell dry weight. the treatment reduced cell hydrophobicity, and reduced the ability of the cells to coaggregate with actinomyces spp. the extract contained about 20 polypeptides and these were identified as being cell-surface components on the basis of one or more of the following criteria: being degraded when cells were incubated with ... | 1986 | 2433382 |
| a collagen-like immunodeterminant on the surface of streptococcus sanguis induces platelet aggregation. | the basis of similarities in the mechanism of human platelet aggregation induced by soluble collagen and the dental plaque bacterium streptococcus sanguis was analyzed. structural and functional comparisons were made by using molecular probes, including rabbit antibody fractions reactive with components on s. sanguis and a synthetic, collagen-like octapeptide mimicking segments from cyanogen bromide fragments 6 and 4 of types i and iii collagen, respectively. when platelets were pretreated with ... | 1987 | 2437190 |
| serological relationships between serotype-iii streptococcus sanguis and lancefield group-h streptococci. | certain strains of streptococcus sanguis and group-h streptococci have been shown to have similar physiological properties and serological specificities. serological studies revealed that serotype-iii s. sanguis shared a common antigen with the so-called "british" group-h streptococci, but not with the "american" group-h streptococci. serotype-iii antigen was extracted in cold 5% trichloroacetic acid from isolated cell walls of s. sanguis atcc 10558, and purified chromatographically. the purifie ... | 1987 | 2438413 |
| association of viridans group streptococci from pregnant women with bacterial vaginosis and upper genital tract infection. | the prevalence and role of viridans group streptococci in the female genital tract have not been well described. in this study of 482 pregnant women, 147 (30%) were culture positive for viridans group streptococci. of 392 women with predominant lactobacillus morphotypes by gram stain (normal), 110 (28%) were colonized with viridans group streptococci, compared with 37 (41%) of 90 women with bacterial vaginosis (bv) (p = 0.02). to determine whether any species were associated with bv, 177 consecu ... | 1988 | 2454943 |
| a comparison of the adhesive properties and surface ultrastructure of the fibrillar streptococcus sanguis 12 and an adhesion deficient non-fibrillar mutant 12 na. | streptococcus sanguis 12 and a naturally occurring mutant, 12 na, were screened for their ultrastructure and adhesive properties in vitro. negative staining showed that strep. sanguis 12 carried three types of surface structure. the majority of cells carried long fibrils that in different batches ranged in length from 80 to 207 nm, and shorter fibrils which were 51.0 +/- 15.7 nm long. both types of fibrils were primarily located at the poles of the cells. occasionally cells were seen that carrie ... | 1989 | 2473974 |
| surface structures, co-aggregation and adherence phenomena of streptococcus oralis and related species. | seven strains of streptococcus oralis were found to possess surface structures. four strains possessed long fimbriae which ranged in length from 266-366 nm, while the remaining three strains possessed shorter peritrichously distributed fibrils which ranged in length from 80-197 nm. the fibrillar strains were morphologically similar to strains of streptococcus sanguis i and ii and streptococcus mitis. no strain of s. oralis produced tufts of fibrils like certain strains of s. sanguis i. strains o ... | 1989 | 2475745 |
| growth inhibition of streptococcus sanguis with alginate. | the experiments of the growth of stre. sanguis were carried out. from the results it was found that the medium including alginate inhibits the growth of stre. sanguis in spite of the fact that the medium does not inhibit the growth of other oral streptococci. | 1989 | 2488883 |
| [lysis of oral streptococci by an extracellular enzyme from the bacterium streptococcus mutans]. | the ability of crude extracellular enzyme produced by the bacterium streptococcus mutans al-7 to lysis the cells of five species, sixteen strains, of oral streptococci was examined. this enzyme showed lytic activity of living cells of only streptococcus sanguis atcc 10558 strain, and of heated cells of streptococcus sanguis atcc 10558 and st-7 strains. the long chains of streptococcus sanguis atcc 10558 was severed at random by this enzyme. early log phase cells of this strain were more sensitiv ... | 1989 | 2489656 |
| [study on air-powder abrasive system for prosthetic clinical application. 3. bacterial adherence to the air-powder abraded metal surface]. | the purpose of this study is to investigate the relation between the metal surface texture and bacterial adherence. the surface of au-ag-pd alloy was treated with or without air-powder abrasion. seven standard strains and two clinical isolates were used. the alloys were mixed with [6-3h]-thimidine labelled bacteria. the radioactivity was measured with a scintilation counter. streptococcus sanguis and clinical isolated bacteroides gingivalis were adhered in a larger amount to the air-powder abrad ... | 1989 | 2489760 |
| effect of tissue-type plasminogen activator (t-pa) on the treatment with benzylpenicillin of rabbits with experimental streptococcus sanguis endocarditis. | | 1989 | 2494270 |
| secretory expression in escherichia coli and bacillus subtilis of human interferon alpha genes directed by staphylokinase signals. | a dna segment covering the signal sequence coding region, the ribosome binding site, and the promoter of the staphylokinase (sak) 42d gene (behnke and gerlach 1987) was cloned into puc19 to form a portable expression-secretion unit (esu). fusion of human interferon alpha 1 (hifn alpha 1) and hybrid hifn alpha 1/2 genes to this sak esu resulted in secretory expression of the two gene products in both escherichia coli and bacillus subtilis. while most of the ifn alpha was exported to the periplasm ... | 1989 | 2505056 |
| [selection of constitutive mutants of gram-positive cocci inducible resistant to macrolides, lincosamides and streptogramins (mls): comparison of the selective effects of the mls]. | mutation frequencies to constitutive resistance were determined for 7 strains inducible resistant to the mls antibiotics (5 staphylococcus aureus, 1 group g streptococcus and 1 streptococcus sanguis). in the 5 staphylococcal strains, mutants were generally selected at a frequency of 10(-9) to 10(-10) (ranging from 4.10(-8) to 9.10(-11) on plates containing 50 mg/l of the following non-inducer mls: spiramycin, josamycin, midecamycin, miocamycin, lincomycin, clindamycin, and pristinamycin factor i ... | 1989 | 2508040 |
| an in vitro study of the antibacterial and antifungal properties of an irreversible hydrocolloid impression material impregnated with disinfectant. | the difficulties associated with the disinfection of alginate (irreversible hydrocolloid) materials have resulted in the development of an irreversible hydrocolloid impression material impregnated with an antimicrobial compound, didecyldimethyl ammonium chloride. this investigation assessed the antibacterial and antifungal properties in vitro of this innovative irreversible hydrocolloid material by using the agar well technique. the microorganisms used were pseudomonas aeruginosa, streptococcus ... | 1989 | 2514263 |
| transferable vancomycin and teicoplanin resistance in enterococcus faecium. | enterococcus faecium bm4165 and bm4178, isolated from immunocompromised patients, one treated with vancomycin, were inducibly resistant to high levels of the glycopeptide antibiotics vancomycin and teicoplanin but susceptible to the new lipopeptide daptomycin (ly146032). strain bm4165 was also resistant to macrolidelincosamide-streptogramin b-type (mls) antibiotics. the genes conferring resistance to glycopeptides and to mls antibiotics in strain bm4165 were carried on plasmids pip819 and pip821 ... | 1989 | 2523687 |
| cloning and expression in escherichia coli of the genes of the arginine deiminase system of streptococcus sanguis nctc 10904. | the common oral bacterium streptococcus sanguis can degrade arginine via the arginine deiminase (ad) system. the three enzymes of this system, ad, ornithine carbamyltransferase (otc), and carbamate kinase (ck), catalyze the breakdown of arginine to ornithine, co2, and two molecules of ammonia, with the production of atp from adp. the genes of the ad system, which are subject to complex regulation in the oral streptococci, have been isolated in bacteriophage lambda by screening for ad activity. t ... | 1989 | 2530176 |
| spontaneous switching of the sucrose-promoted colony phenotype in streptococcus sanguis. | streptococcus sanguis on media containing 3% sucrose gives rise to characteristic hard cohesive colonies (designated spp+). populations of spp+ bacteria (strain challis) on sucrose media switch to a soft noncohesive phenotype (designated spp-) at a frequency of 10(-4) to 10(-3). spp- bacteria switch back to spp+ bacteria at a similar frequency. successive rounds of spp variation were observed. the spp phenotypic switch was associated with changes in extracellular glucosyltransferase activity. | 1989 | 2530177 |
| in vitro adherence of bacteria to sutures in cardiac surgery. | the adherence of bacteria to sutures used in cardiac surgery was studied by in vitro quantitative determination with [3h]-leucine-labeled staphylococcus epidermidis, staphylococcus aureus and streptococcus sanguis. the adherence per unit area for staphylococci was least for monofilament polypropylene (prolene), 3 times higher (p less than 0.05) for braided polyester (mersiline) and greatest (10 times, p less than 0.005) for braided polyester sutures coated with polybutylate (ethibond). mean valu ... | 1989 | 2530235 |
| low back pain as the only presenting symptom in streptococcus sanguis endocarditis. | | 1989 | 2530612 |
| [glucosyltransferase (gtf) and immunization against dental caries in humans]. | glucosyltransferase (gtf) is an extracellular or cell-associated enzyme synthesized by the "mutans" group of streptococci as well as the s. sanguis and is responsible for the biosynthesis of extracellular polysaccharides. glucan formation in dental plaque mediates binding of s. mutans and s. sanguis to one another as well as to other bacteria. although the production of organic acids is an indispensable property of the cariogenic strains of bacteria, their ability to stick and accumulate on toot ... | 1989 | 2534848 |
| isolation and characterization of the streptococcus mutans gtfd gene, coding for primer-dependent soluble glucan synthesis. | two glucosyltransferase genes from streptococcus mutans gs-5, gtfb and gtfc, have been previously isolated and sequenced in this laboratory. in the present communication a third gtf gene, gtfd, was isolated and characterized. isolation of the gene involved a novel procedure utilizing the integration plasmid pva891. a peptide expressed by the 1.7-kilobase dna fragment from strain nhs1 (containing deletions in both the gtfb and gtfc genes) was initially identified in a puc18 clone bank with antigl ... | 1989 | 2543630 |
| daptomycin (ly146032) for prevention and treatment of experimental aortic valve endocarditis in rabbits. | the efficacy of daptomycin (ly146032), a vancomycinlike lipopeptide antibiotic, was compared with that of antibiotics commonly in use for prevention and treatment of experimental aortic valve endocarditis in rabbits. strains of staphylococcus aureus. s. epidermidis, streptococcus sanguis, and enterococcus faecalis were used to establish endocarditis. a single 10-mg/kg dose of daptomycin and a single 25-mg/kg dose of vancomycin were both effective in prevention of endocarditis produced by strains ... | 1989 | 2554799 |
| interaction of streptococcal lipoteichoic acid with artificial tooth pellicle. | artificial pellicles were prepared by coating hydroxyapatite beads with whole saliva. radiolabelled lipoteichoic acid was isolated from streptococcus sanguis nctc 7863 by phenol extraction. various concentrations of radiolabelled lipoteichoic acid were mixed with saliva-coated hydroxyapatite in the presence and absence of high ionic strength phosphate buffer, bovine serum albumin, gelatin, unlabelled lipoteichoic acid, tween 20 and triton x-100. the amount of lipoteichoic acid binding was measur ... | 1989 | 2556987 |
| bacteroides gingivalis vesicles mediate attachment of streptococci to serum-coated hydroxyapatite. | outer membrane vesicles purified from bacteroides gingivalis culture supernatant bound to serum coated hydroxyapatite (seha). the immobilized vesicles served as receptors for a number of species of oral streptococci. the binding of streptococcus sanguis 12 to seha was increased 10 times by the vesicles. vesicle-associated binding increased proportionally with an increase in the number of bound vesicles. arginine and lactose both partially reduced binding of s. sanguis. heating the vesicles destr ... | 1989 | 2561775 |
| dna probe for identification of streptococcus pneumoniae. | a total of 287 clinical isolates of streptococcus pneumoniae (pneumococcus) were tested for their ability to undergo autolysis when treated with sodium deoxycholate. the test was positive for all but one isolate, strain doc-1. this autolysis required the activity of an enzyme which is unique and characteristic of s. pneumoniae: a choline-dependent n-acetylmuramoyl-l-alanine amidase, the gene product of the lyta gene. we used lyta as a dna probe to test the distribution of the autolysin gene amon ... | 1989 | 2563386 |
| fimbria-associated adhesin of bacteroides loeschei that recognizes receptors on procaryotic and eucaryotic cells. | inhibition studies with a set of adhesin-specific monoclonal antibodies and various sugars revealed that a fimbria-associated adhesin of bacteroides loeschei recognizes receptors on both procaryotic and eucaryotic cells. these interactions permit this bacterium to attach to both types of cells, producing coaggregates in the presence of strains of streptococcus sanguis and hemagglutination in the presence of neuraminidase-treated human erythrocytes. | 1989 | 2569449 |
| nucleotide sequence analysis of a type 1 fimbrial gene of streptococcus sanguis fw213. | a structural gene for type 1 fimbriae of streptococcus sanguis fw213 was located within a 6-kilobase fragment cloned in escherichia coli. a 1.6-kilobase internal fragment contains an open reading frame of 927 bases coding for an immunoreactive peptide of 34,349 daltons, which corresponds in size with an observed cytoplasmic form of fimbrial peptide (p. m. fives-taylor, f. l. macrina, t. j. pritchard, and s. j. peene, infect. immun. 55:123-128, 1987). disruption of the reading frame by insertiona ... | 1989 | 2572555 |
| bacteroides loeschei pk1295 cells express two distinct adhesins simultaneously. | bacteroides loeschei synthesizes two distinct adhesins that mediate its coaggregation with streptococcus sanguis 34 and actinomyces israeli pk14. streptococcal adhesin-specific and actinomyces adhesin-specific monoclonal antibodies were used to prepare antibody-coated 5- or 10-nm gold particles. these were used in immunoelectron microscopic studies to establish that essentially all bacteroides cells in a population express both adhesins. in general, the two sizes of gold particles representing e ... | 1989 | 2572563 |
| the potential hazard of using multiple-dose heparin and insulin vials in continuous ambulatory peritoneal dialysis. | | 1989 | 2572637 |
| [the immunological basis of vaccination against carious disease. microorganism interactions. specific host defense system. immunological protection of the oral cavity]. | the authors describe the very complex interactions of the microorganisms of the oral cavity and among these latter and the guest organism. there are described the mechanisms of aspecific defense of the oral cavity and give some outlines of the anatomy and of the physiology of the organs and of the glandular structures of the specific immune defence of the oral cavity through blood and saliva. | 1989 | 2577119 |
| reduction of acidurance of streptococcal growth and glycolysis by fluoride and gramicidin. | the acidurance of glycolysis by intact cells of streptococcus mutans gs-5, streptococcus salivarius atcc 25925, and streptococcus sanguis nctc 10904 was found to be highly dependent on membrane functions affected by gramicidin, which increases the proton permeability of cell membranes. plots of % glucose utilized during two hours against suspension ph values for cells suspended in 100 mm phosphate buffer plus 1 mm mgcl2 plus 13.9 mm glucose indicated, for 50% glucose utilization, ph values of 5. ... | 1985 | 2579114 |
| the effects of basic and acidic synthetic polypeptides on the adherence of the oral bacteria, streptococcus mutans and streptococcus sanguis, to hydroxyapatite. | two basic and two acidic synthetic polypeptides that bind strongly to hydroxyapatite at neutral ph were tested to determine their influence on adsorption of two streptococcus mutans and two streptococcus sanguis strains to hydroxyapatite. the adsorption of the strains was significantly enhanced or reduced by the basic and acidic agents, respectively. study of acidic polypeptides provided evidence of competition between the polypeptides and the bacterial cells for hydroxyapatite adsorption sites. | 1985 | 2581534 |
| the complete structure of the capsular polysaccharide from streptococcus sanguis 34. | a complete structure for the capsular polysaccharide of streptococcus sanguis 34, which is responsible for coaggregation of this bacterium with actinomyces viscosus t14v, an important step in the formation of dental plaque, is proposed, based partly on the 1h-n.m.r. spectrum, which was assigned by 2-dimensional cosy, homonuclear hartmann-hahn spectroscopy, and nuclear overhauser effects. a phosphoric diester linkage was identified from the 31p-n.m.r. spectrum, and the linkage was determined from ... | 1989 | 2582463 |
| evaluation of antimicrobial activity in vitro of ten root canal sealers on streptococcus sanguis and streptococcus mutans. | the antimicrobial activity of ten root canal sealers was studied on streptococcus sanguis and streptococcus mutans. one hundred plates of trypticase soy agar-sheep red blood cell (10%), each with four distinct 3 mm diameter wells, were divided into two groups of 50 by means of the lawn technique; one group was inoculated with s. sanguis and the other with s. mutans. four milligrams of each root canal sealer was placed in each of three wells in 10 plates (five plates of each inoculant); the fourt ... | 1989 | 2594327 |
| procoagulant activity of endocardial vegetations and blood monocytes in rabbits with streptococcus sanguis endocarditis. | to investigate activation of the coagulation system in bacterial endocarditis, we determined the procoagulant activity of blood monocytes isolated from rabbits with streptococcus sanguis-infected or sterile catheter-induced endocardial vegetations. this activity was determined directly after isolation from the peripheral blood and after stimulation in vitro by either endotoxin or by phagocytosis of s. sanguis. the procoagulant activity of the vegetations of these rabbits was also determined. the ... | 1989 | 2595656 |
| a study of in vitro attachment of streptococcus sanguis and actinomyces viscosus to saliva-treated titanium. | this study examined the initial attachment of streptococcus sanguis g9-b and actinomyces viscosus t14v to saliva-treated powdered enamel and titanium surfaces. using an in vitro adherence model, significantly lower numbers of actinomyces viscosus t14v bound to the saliva-treated titanium surface when compared to that of the similarly treated enamel. the binding of streptococcus sanguis g9-b to titanium or enamel did not vary significantly. a comparison of the percentage of cells bound to the tit ... | 1989 | 2599579 |
| the microflora associated with the development of initial enamel decalcification below orthodontic bands in vivo in children living in a fluoridated-water area. | thirty-four caries-free teeth destined for orthodontic extraction were banded to provide a protected area for the accumulation of plaque. the teeth were extracted at one, two, four, eight, and 14 days after being banded, and samples of the flora below the band were analyzed for the presence of streptococcus sanguis, streptococcus 'mitior', 'mutans streptococci', actinomyces viscosus, actinomyces naeslundii, actinomyces odontolyticus, lactobacillus species, and veillonella. after plaque sampling, ... | 1989 | 2600252 |
| effects of varnishes containing chlorhexidine on the human dental plaque flora. | this study describes the effects of varnishes containing 0%, 10%, 20%, or 40% chlorhexidine diacetate on the microflora of human fissure dental plaque. sandarac, a natural resin, was used as the varnish base. ten subjects, each with at least four sound fissures harboring high levels of streptococcus mutans, participated in the study. the fissures in each of the individuals were randomly assigned to four experimental groups, in each of which one of the varnishes was tested. the varnish treatment ... | 1989 | 2600262 |
| cultural and electronmicroscopic studies of the effect of penicillin on tolerant oral streptococci. | penicillin-tolerant and -sensitive strains of oral streptococci were treated with penicillin to determine the production of a post-antibiotic effect (pae). no pae was seen with any of the s. sanguis strains tested but most strains of the other oral streptococcal species produced a pae. cultures on nitrocellulose filters treated with penicillin were examined by scanning electronmicroscopy and showed that tolerant and sensitive strains lost the ability to adhere to the filter after application of ... | 1989 | 2600959 |
| a study of the dental health of patients undergoing heart valve surgery. | the mouth is a possible source of infection in patients receiving prosthetic heart valves. in this study, the dental health of such patients was found to be very poor. whilst most of the patients were asymptomatic, only two were dentally fit and 65% required the extraction of one or more teeth. on average, patients had not attended a dental practice for more than 9 years. this level of dental neglect, in a group of patients particularly at risk, is alarming and this study indicates that a higher ... | 1989 | 2602235 |
| a comparison between the elemental surface compositions and electrokinetic properties of oral streptococci with and without adsorbed salivary constituents. | in order to characterize the functional cell surface, isoelectric points and elemental surface concentration, the ratios of nitrogen, oxygen and phosphorus to carbon of saliva-coated strains were determined by ph-dependent zeta potential measurements and x-ray photoelectron spectroscopy and compared with those of uncoated strains. the measurements of potential were carried out on completely hydrated cells, whereas the spectroscopy was on freeze-dried micro-organisms. the small increase in the ni ... | 1989 | 2610623 |
| osteomyelitis associated with chronic periodontitis: a report of three cases. | correctly differentiating between periodontal abscess (localized, acute suppurative infection of the periodontium) and osteomyelitis (the extension of an infection into the bone medullary cavity) is crucial since the former may not require antibiotics for resolution, while the latter will. initial assessment and treatment of osteomyelitis should be based on clinical examination, radiographic interpretation, and experience. three case histories of osteomyelitis are presented, ranging from a perio ... | 1989 | 2614636 |
| meningitis following bacteremia with streptococcus sanguis. | | 1989 | 2626226 |
| properties of a phosphocarrier protein (hpr) extracted from intact cells of streptococcus sanguis. | cells of streptococcus sanguis strain challis were incubated with sodium lauroylsarcosinate to extract surface proteins. a polypeptide of apparent molecular mass 16 kda comprising about 12% of the extract was purified using anion-exchange chromatography. the polypeptide was shown to be a phosphocarrier protein (hpr) that could also be found in the soluble (cytoplasmic) fraction from cells broken by homogenization with glass beads. in vivo labelling of s. sanguis cells with 32pi showed that the p ... | 1989 | 2636256 |
| [chemiluminescence and phagocytic reactions of human nuclear leukocytes incubated with streptococcus sanguis]. | this experiment was designed to determine the role of indigenous organisms in gingival inflammation of the oral cavity, the test bacteria consisted of 3 strains of streptococcus sanguis isolated from plaque at the gingival margin and grown in sure culture. these strains were subsequently designated as a, b and c. peripheral nuclear leukocytes were isolated from 5 test subjects with clinically healthy gingiva. luminol-dependent chemiluminescence and phagocytic functions of the 3 strains were meas ... | 1989 | 2637295 |
| technical note: antimicrobial action of glass-ionomer lining cement on s. sanguis and s. mutans. | there are a number of glass-ionomer lining cements available to the profession. it is generally agreed that the glass-ionomer cements demonstrate reasonably good biocompatibility. the purpose of this study was to evaluate the antimicrobial activity of six glass-ionomer cements on s. sanguis and s. mutans. forty-eight plates of tsa-srbc (10%), each with 3.0-mm-diam. wells, were divided into two groups (1 and 2) of 24 each. group 1 was inoculated with s. sanguis (10558) and group 2 with s. mutans ... | 1989 | 2638272 |
| fibrinogen binding and resistance to phagocytosis of streptococcus sanguis expressing cloned m protein of streptococcus pyogenes. | the biological properties of streptococcus pyogenes m protein cloned and expressed in s. sanguis were investigated. the spm-5 gene previously cloned into escherichia coli was subcloned into the e. coli-s. sanguis shuttle plasmid pva838 to produce a newly constructed plasmid, pbk100. cells of s. sanguis transformed with pbk100 expressed 53-, 55-, and 58-kilodalton polypeptides reacting with type 5 m protein antiserum in immunoblots. the m protein was expressed on the surface of s. sanguis cells a ... | 1989 | 2642469 |
| saliva-mediated aggregation of enterococcus faecalis transformed with a streptococcus sanguis gene encoding the ssp-5 surface antigen. | the interaction of a high-molecular-weight salivary glycoprotein (agglutinin) with streptococcus sanguis m5 leads to the formation of bacterial aggregates. we have previously shown that the ssp-5 surface antigen from s. sanguis m5 binds the salivary agglutinin and therefore may be involved in the aggregation process. here we report the transformation of a nonaggregating enterococcus faecalis strain with the ssp-5 gene and show that the protein is expressed on the cell surface and confers an aggr ... | 1989 | 2651309 |
| a double-blinded comparative evaluation of three media for chromophore testing with viridans and nutritionally variant (deficient) streptococci. | a double-blinded prospective comparison of chromophore testing among 109 clinical isolates of alpha-hemolytic gram-positive cocci and 48 strains of nutritionally variant (deficient) streptococci (nvs) for its sensitivity, specificity, and predictive value under stimulated clinical laboratory conditions was performed after growth in three different media--todd-hewitt broth supplemented with 10 micrograms/ml of pyridoxal (thbp), thbp with 0.8% (w/v) yeast-extract (thbp + ye), and a semisynthetic c ... | 1989 | 2655426 |
| characteristics of a protease of streptococcus sanguis g9b which degrades the major salivary adhesin. | an endogenous enzyme present in cell surface extracts of streptococcus sanguis strain g9b degraded the major salivary adhesin of the organism. the enzyme showed optimal activity between 50 and 65 degrees c and was inactivated at higher temperatures. the activity at these unusually high temperatures seemed to be a consequence of release from the cell surface since intact whole g9b cells showed greater activity at 37 degrees c. the enzyme was not found in culture supernatants of g9b cells. the ph ... | 1989 | 2656394 |
| relationship of subgingival plaque flora to lysosomal and cytoplasmic enzyme activity in gingival crevicular fluid. | examining the relationships among indicators of the acute inflammatory response in gingival crevicular fluid (gcf) and specific bacterial species in subgingival plaque may provide indications of which bacterial species or groups of species may be associated with potentially destructive host-derived processes. here we report on the relationship of the subgingival plaque flora to the activity of mammalian forms of the enzymes beta-glucuronidase (beta g), lactate dehydrogenase (ldh), and arylsulfat ... | 1989 | 2656765 |
| iga1 proteases of oral streptococci: ecological aspects. | the ecology and identity of iga1 protease-producing streptococci in the human oral cavity were studied in an attempt to obtain insight into the significance of iga1 proteases for host-parasite relationships. contrary to previous observations, a detailed taxonomic analysis of oral streptococci revealed that all strains of s. sanguis and s. oralis ("s.mitior") produced iga1 protease. in addition, iga1 protease activity was observed in some isolates of s.mitis. of the streptococci that initiate pla ... | 1989 | 2659509 |
| isolation of a coaggregation-inhibiting cell wall polysaccharide from streptococcus sanguis h1. | coaggregation between streptococcus sanguis h1 and capnocytophaga ochracea atcc 33596 cells is mediated by a carbohydrate receptor on the former and an adhesin on the latter. two methods were used to release the carbohydrate receptor from the gram-positive streptococcus, autoclaving and mutanolysin treatment. the polysaccharide released from the streptococcal cell wall by either treatment was purified by ion-exchange chromatography; this polysaccharide inhibited coaggregation when preincubated w ... | 1989 | 2661543 |
| comparison of different assays for the aggregation of oral bacteria by human whole saliva. | for comparison, human whole saliva-induced aggregation was studied by phase-contrast microscopy, spectrophotometry combined with macroscopic observations, and in microtiterplate assay under identical experimental conditions for actinomyces viscosus hg 85 (t14-v) and hg 380 (t14-av), bacteroides gingivalis hg 66 (w 83), streptococcus rattus hg 59 (bht), and streptococcus sanguis i hg 169. the entire process of formation, extension, and sedimentation of aggregates could merely be observed by the c ... | 1989 | 2662902 |
| the streptokinase gene of group a streptococci: cloning, expression in escherichia coli, and sequence analysis. | the gene specifying the group a streptokinase (ska) gene was cloned from an m type 49 strain of streptococcus pyogenes and shown to express in escherichia coli. the nucleotide sequence of the dna fragment carrying ska was determined and compared to that of the group c streptokinase gene (skc). there is 90% sequence identity between the two genes, with highly conserved transcription and translation control regions. the deduced amino acid sequence of the group a streptokinase (ska) contains the sa ... | 1989 | 2668686 |
| lingual abscess: the value of ultrasound in diagnosis. | a case of lingual abscess is presented. the sparsity of physical signs made ultrasound imaging an invaluable investigation in the diagnosis. | 1989 | 2685149 |
| novel shuttle vectors for improved streptokinase expression in streptococci and bacterial l-forms. | novel shuttle vectors of small size and increased copy number capable of replication in escherichia coli, l-forms of proteus mirabilis, and streptococci were constructed from a streptococcal erythromycin-resistant plasmid and an escherichia coli phasmid. the streptokinase gene, skc, was inserted into one of them, and skc expression was studied in streptococcus sanguis, streptococcus lactis, and in an l-form strain (lvi) of proteus mirabilis. the new streptokinase shuttle plasmid, pmls10 (7.3 kb) ... | 1989 | 2693202 |
| cloning and expression of an adhesin antigen of streptococcus sanguis g9b in escherichia coli. | a genomic library of streptococcus sanguis, strain g9b, was constructed and expressed in escherichia coli using a lambda gt11 expression vector. the amplified library was probed with polyclonal anti-g9b igg and 13 antigen-positive clones were isolated. a lysate of one clone, designated pp39, absorbed the adhesion-inhibitory activity of anti-g9b igg. this clone contained an insert of approximately 2000 bp and expressed unique 200 and 53 kda proteins that reacted with monospecific anti-adhesin ant ... | 1989 | 2695596 |
| [in vitro study of bacterial invasion in radicular dentin]. | the purpose of this study was to investigate whether plaque bacteria invade exposed radicular dentin after root planing or chemical root treatment in vitro. pieces of dentin were cut out from impacted third molars. the surface of all dentin pieces was treated with sandpaper (#240) so as to make the surface roughness of dentin pieces equal to that of the root surface after root planing (rp surface). half of the dentin pieces were treated with citric acid (ph 1.0) for 3 minutes (ca surface). after ... | 1989 | 2700355 |
| alveolar epithelial responses in experimental streptococcal pneumonia. | we describe an experimental model of pneumonia in wistar rats evoked by streptococcus sanguis. the lesion developed rapidly as a confluent bronchopneumonia of the single-lobed left lung. except at the extreme base, where an abscess formed, the pneumonic process thereafter resolved, and most of the lung appeared microscopically and ultrastructurally normal 8 days after infection. sequential electron microscopic studies revealed that in the areas of lung which subsequently resolved, damage was res ... | 1989 | 2715880 |
| molecular analysis of viridans and nutritionally deficient (variant) streptococci causing sequential episodes of endocarditis in a patient. | clinical blood isolates from sequential episodes of endocarditis occurring over a six-month period of time in an addict were investigated. the pathogens were streptococcus sanguis ii, streptococcus mitis, and a nutritionally deficient (variant) streptococcus. the authors determined the dna relatedness of these isolates by antibiograms, plasmid profiles, chromosomal endonuclease restriction digestions, and dot blot dna-dna hybridization analyses. the s. sanguis ii and nutritionally deficient stre ... | 1989 | 2718964 |
| detachment of oral bacteria from saliva-coated hydroxyapatite by human polymorphonuclear leukocytes. | human polymorphonuclear leukocytes (pmn) demonstrated the ability to detach actinomyces viscosus, a. naeslundii and streptococcus sanguis from saliva-coated hydroxyapatite beads (sha). between 60 to 80% of bacteria were detached within 1 hour at pmn-to-bacteria ratios between 1:10 to 1:22. detachment was enhanced by treating bacteria with fresh but not heat-inactivated normal human serum. detachment of serum-treated a. viscosus was inhibited by cytochalasin b, l-1-tosylamide-2-phenylethylchlorom ... | 1989 | 2724035 |
| identification of viridans streptococci by pyrolysis-gas chromatography. | an isothermal method of pyrolysis-gas chromatography (py-gc) was used for the identification of viridans streptococci. pyrograms from 104 reference strains were subjected to a discriminant analysis to produce classification coefficients for the identification of 74 test organisms. five groups representing recognised species were discriminated but streptococcus milleri strains could not be distinguished from s. sanguis. if s. milleri and s. sanguis are regarded as a single pyrogroup, only three s ... | 1989 | 2724324 |
| molecular surface characterization of oral streptococci by fourier transform infrared spectroscopy. | in order to characterize the molecular composition of oral streptococci, infrared transmission spectroscopy on freeze-dried cells dissolved in kbr was used. all infrared spectra show similar absorption bands for the strains studied with the most important absorption bands located at 2930 cm-1 (ch), 1653 cm-1 (ami), 1541 cm-1 (amii) and two bands at 1236 cm-1 and 1082 cm-1, which were assigned to phosphate and sugar groups. however, calculation of absorption band ratios normalized with respect to ... | 1989 | 2730916 |
| production of mutacin-like substances by streptococcus mutans. | production of inhibitory substances by strains of the streptococcus mutans group is well documented, but the nature of the substances implied is often unknown. of nine laboratory strains known to produce inhibitory substances, the optimal conditions for producing inhibition zones on solid media were found to vary between strains but good production was generally obtained on all-purpose media with tween 80 at 37 degrees c after 2-4 days of aerobic incubation. streptococcus sanguis ny101 was found ... | 1989 | 2731078 |
| roxithromycin as a possible agent for prophylaxis of endocarditis: a study in normal volunteers. | a single dose of roxithromycin, 300 mg, was taken by six healthy male volunteers on three occasions at consecutive weekly intervals. it was well tolerated. on the first two occasions, roxithromycin was assayed in serum samples taken at intervals up to 8 h after the administration. the mean peak concentration at 1 h was 3.0 mg/l (range 0.3-7.3). the serum samples from the volunteers showed variable bactericidal activity against a strain of streptococcus sanguis isolated from a case of bacterial e ... | 1989 | 2732123 |
| root canal microflora: qualitative changes after endodontic instrumentation. | the changes in the pattern of bacteria in root canal after repeated washing with naocl and endodontic instrumentation were evaluated. samples were obtained from the root canals of patients before and after endodontic instrumentation, and analyzed according to the usual microbiological techniques. washing with naocl and endodontic instrumentation act against bacteria such as peptostreptococci, streptococcus sanguis, actinomyces israelii, proteus mirabilis, bacteroides, whereas streptococcus lacti ... | 1989 | 2732777 |
| aerococcus-like organism--an unnoticed urinary tract pathogen. | aerococci are only occasionally isolated from urinary tract specimens. a 4-month' survey revealed 29 patients suspected of urinary tract infection (uti), from whom an aerococcus-like organism (alo) was isolated in significant numbers from urinary tract specimens and singly in 11 cases. approximately 0.8% of all urinary tract specimens examined during the period yielded growth of alo. the median age of patients was 75 years. half of the patients were found to have conditions predisposing to uti. ... | 1989 | 2736106 |
| effect of neuraminidase on the adherence to salivary pellicle of streptococcus sanguis and streptococcus mitis. | neuraminidase-sensitive adherence to experimental salivary pellicles was studied using eight strains of streptococcus sanguis and five strains of streptococcus mitis. approximately 60% of the strains of each species showed significantly lower adherence to neuraminidase-treated versus untreated saliva-coated hydroxyapatite. in addition, the adherence of several of these streptococcal strains to saliva-coated hydroxyapatite and neuraminidase-treated saliva-coated hydroxyapatite was inhibited using ... | 1989 | 2736574 |
| antimicrobial and antiplaque effects of a chlorhexidine and triton x-100 combination. | this study aimed to assess the in vitro antimicrobial effect and in vivo plaque-inhibiting capacity of chlorhexidine (ch) combined with the nonionic detergent triton x-100. synergistic inhibition was observed by the combination of triton x-100 and ch on in vitro growth of s. sobrinus omz 176 and of s. sanguis 10556. in the clinical experiments, 10 subjects rinsed twice daily with 10 ml aqueous solutions of 11.6 mm triton x-100, and 0.55 mm ch, and with a combination of the two agents. all mechan ... | 1989 | 2740834 |
| determination of viridans streptococci surface lipoteichoic acid by enzyme linked immune sorbent assay. | an enzyme linked immune sorbent assay (elisa) was developed to measure bacterial surface lipoteichoic acid (lta). numerous strains of oral streptococci belonging to the 'viridans' group were examined on three separate occasions. the results show that, under these cultural conditions, oral streptococci do not normally express lta on the cell surface. occasionally strains produced amounts of lta detectable using the elisa but this was not a reproducible phenomenon. | 1989 | 2744420 |
| microflora in plaque from approximal posterior composite resin restorations. | | 1989 | 2756097 |
| determination of the absolute configuration of the sugar residues of complex polysaccharides by circular dichroism spectroscopy. | circular dichroism spectroscopy is used to determine the absolute configuration of the constituent sugar residues of bacterial polysaccharides from two strains of streptococcus sanguis which are important receptors in bacterial coaggregation in the formation of dental plaque. a high-pressure liquid chromatographic method for carbohydrate analysis of glycoproteins, glycolipids, and polysaccharides has been extended to sugar chirality determination by collection of the eluted hplc fractions and su ... | 1989 | 2757197 |
| bacterial meningitis following epidural anesthesia for vaginal delivery: a case report. | acute bacterial meningitis following epidural anesthesia is a rare event. we describe a case in which a young woman received epidural anesthesia for vaginal delivery. the initial attempt at placement of the epidural resulted in entry into the subarachnoid space. the patient's postpartum course was complicated by persistent headache. she received epidural blood patch on two occasions, but her headache continued and she developed persistent fever. the diagnosis of acute bacterial meningitis was ma ... | 1989 | 2761926 |
| the microbiota of early periodontitis lesions in adults. | the predominant cultivable microbiota of shallow progressive (active) periodontal lesions in 4 adults (29-40 years) was compared to that of shallow inactive sites. periodontal attachment levels were monitored longitudinally, and sites showing 2-3 mm attachment loss in the previous 1-2 months were considered disease active. inactive sites showed no change in attachment level. the microbiota of shallow sites was dominated by gram negative rod species including wolinella recta, bacteroides forsythu ... | 1989 | 2768541 |
| the effect of chlorhexidine on streptococcus sanguis biofilms. | the bactericidal effect of chlorhexidine on streptococcus sanguis biofilms was investigated and compared with its effect on suspensions of the organism. the biofilms were found to be more resistant to the bactericidal effects of chlorhexidine than the bacterial suspensions in terms of the number of surviving organisms. the minimum inhibitory concentration (mic) being 25 and 50 micrograms/ml, respectively, for the suspension and the 24 h biofilm. the age of the biofilm had a marked effect on the ... | 1989 | 2770556 |
| [frequency and severity of systemic infections caused by streptococcus mitis and sanguis ii in neutropenic children]. | we have retrospectively evaluated 24 sepsis episodes caused by viridans streptococci in 23 neutropenic children during a 21 months period at the pediatric hematology unit of st. louis hospital. the underlying malignancies included acute lymphoblastic leukemia, acute non lymphoblastic leukemia, aplastic anemia and solid tumor. in 17 children neutropenia, defined as a neutrophil count of less than 500 per cubic millimeter, was caused by cytotoxic chemotherapy. for 6 other children neutropenia was ... | 1989 | 2780102 |
| characterization of salivary alpha-amylase binding to streptococcus sanguis. | the purpose of this study was to identify the major salivary components which interact with oral bacteria and to determine the mechanism(s) responsible for their binding to the bacterial surface. strains of streptococcus sanguis, streptococcus mitis, streptococcus mutans, and actinomyces viscosus were incubated for 2 h in freshly collected human submandibular-sublingual saliva (hsmsl) or parotid saliva (hps), and bound salivary components were eluted with 2% sodium dodecyl sulfate. by sodium dod ... | 1989 | 2788139 |
| lactate influx and efflux in the 'streptococcus mutants group' and streptococcus sanguis. | lactate influx was measured in streptococcus sanguis and in several strains of streptococcus mutans by comparing the intra- and extracellular distribution of (14c)-lactate. lactate efflux was followed enzymatically against rising external lactate concentration. the glucose concentration was monitored in the same way. with s. sanguis omz 9, lactate was transported into the cells when a high external lactate concentration and a ph gradient were established. the transport rate was approximately 1,0 ... | 1989 | 2790859 |
| water-soluble glucans from streptococcus mutans strain ing-britt as an energy source for bacterial growth. | two fractions of water-soluble glucans with different molecular weight were produced by streptococcus mutans ib. the larger glucan had a molecular weight of about 40,000. the molecular weight of the small glucan was estimated to be 4,100 by gel filtration chromatography and by biochemical methods. these glucans were tested for their ability to initiate and support growth of s. mutans ib and streptococcus sanguis 903. s. sanguis 903 could grow with the low molecular weight glucan at a reduced rat ... | 1989 | 2790860 |
| plaque inhibition by a combination of zinc citrate and sodium lauryl sulfate. | bacteriological tests demonstrated an additive inhibitory effect of zncl2 and sodium lauryl sulfate (sls) on in vitro growth of streptococcus sobrinus omz 176 and of streptococcus sanguis atcc 10556. as measured by atomic absorption spectrophotometry, the solubility of zinc citrate increased in the presence of sls. after 48 h, the concentration of solubilized zinc from aqueous solutions of 5.0 mm zinc citrate was 12.0 mm versus 14.4 mm in the presence of 34.7 mm sls. the plaque-inhibiting proper ... | 1989 | 2790864 |
| prognosis of ruptured intracranial mycotic aneurysms: a review of 12 cases. | intracranial mycotic aneurysms (ima) occur in 1-3% of all infective endocarditis. although spontaneous resolution was evidenced on serial angiograms in many asymptomatic cases, the prognosis, if they rupture, is reported to be worse and partly contingent on the therapeutic approach. among 12 patients (six acute and six subacute endocarditis) with ruptured ima, six were treated surgically and four were treated medically. two patients died during rupture before any treatment could be started. six ... | 1989 | 2806280 |
| effect of sodium and amine fluoride treatment on adsorption and ultrastructure of s. mutans and s. sanguis. | a new modification of hydroxyapatite (ha) adherence assay is presented to study the effect of two chemically different fluorides, sodium fluoride (2% solution) and amine fluoride (elmex sol. concentrate) on the adsorption of s. mutans (atcc 27351) and s. sanguis (atcc 10556). vaseline-coated glass rods were rolled in ha powder, after which the rods were either coated or not coated with salivary pellicle. both strains adsorbed better to saliva-coated rods. pretreatment of the rods with fluorides ... | 1987 | 2821611 |
| evidence for natural gene transfer from gram-positive cocci to escherichia coli. | high-level resistance to macrolide-lincosamide-streptogramin type b (mls) antibiotics in escherichia coli bm2570 is due to the presence on the conjugative plasmid pip1527 of the mls resistance determinant ermbc, which is almost identical to the erm genes previously described in plasmid pam77 from streptococcus sanguis (ermam) and in transposon tn917 from enterococcus faecalis (ermb). this gene and its regulatory region are located downstream from the insertion sequence is1. the 23s rrna methylas ... | 1988 | 2832378 |
| host-vector system for integration of recombinant dna into chromosomes of transformable and nontransformable streptococci. | we describe a genetic system in which transformation of streptococcus pneumoniae and streptococcus sanguis was used to insert recombinant dna into the conjugative chromosomal element omega (cat tetm) 6001 (omega 6001). the element containing the recombinant dna was then transferred by conjugation to the chromosome of transformable and nontransformable streptococci. when escherichia coli plasmid pdp36 was used as donor in transformation, it was capable of inserting 5.9 kilobases of heterologous d ... | 1988 | 2832394 |
| intergeneric and intrageneric conjugal transfer of plasmid-encoded antibiotic resistance determinants in leuconostoc spp. | transfer of the broad-host-range resistance plasmids pip501 and pam beta 1 from streptococcus faecalis to leuconostoc dextranicum and leuconostoc cremoris occurred between cells that were immobilized on nitrocellulose filters in the presence of dnase. transfer of pip501 to leuconostoc spp. also occurred when streptococcus sanguis and streptococcus lactis were used as donors. in addition, transfer of pip501 and pam beta 1 was observed from l. cremoris and l. dextranicum transconjugants to s. sang ... | 1988 | 2833158 |
| cloning and characterization of the tetracycline resistance determinant of and several promoters from within the conjugative transposon tn919. | tn919 is a 15- to 16-kilobase (kb) tetracycline resistance conjugative transposon that was originally isolated from streptococcus sanguis fc1. the tetracycline resistance determinant (tet) was found on a 4.2-kb hindii fragment by in vitro deletion analysis. this fragment was subcloned to a pwv01 origin capable of directing replication in escherichia coli, bacillus subtilis, and streptococcus lactis, and expression was observed in all three genera. in all cases, expression was weaker when only th ... | 1988 | 2839111 |
| nucleotide sequence analysis of tetracycline resistance gene teto from streptococcus mutans dl5. | streptococcus mutans dl5, isolated from the dental plaque of a pig, was resistant to high levels of streptomycin (sm, 20 mg/ml), erythromycin (em, 1 mg/ml), and tetracycline (tc, greater than 100 micrograms/ml), but contained no detectable plasmid dna. the smr and emr determinants were cloned from cellular dna on the self-replicating 5-kilobase-pair (kbp) ecori fragment of pam beta 1 and the 4.2-kbp cryptic plasmid pva380-1, respectively, by transformation of streptococcus sanguis challis. helpe ... | 1988 | 2841293 |
| characterization of an adhesion antigen of streptococcus sanguis g9b. | an antigen possessing the attributes of an adhesion has been identified in streptococcus sanguis g9b. cell surface components were extracted from g9b and a spontaneously occurring nonadherent mutant of g9b, strain adh-, with a 2 mm barbital buffer, ph 8.6. the extract of g9b but not of adh- absorbed more than 80% of the adhesion-inhibitory activity of anti-g9b immunoglobulin g (igg). immunoblots revealed 80- and 52-kilodalton (kda) antigens present in the g9b extract but not in the adh- extract. ... | 1988 | 2842262 |
| role of the arginine deiminase system in protecting oral bacteria and an enzymatic basis for acid tolerance. | the arginine deiminase system was found to function in protecting bacterial cells against the damaging effects of acid environments. for example, as little as 2.9 mm arginine added to acidified suspensions of streptococcus sanguis at a ph of 4.0 resulted in ammonia production and protection against killing. the arginine deiminase system was found to have unusual acid tolerance in a variety of lactic acid bacteria. for example, for streptococcus rattus fa-1, the ph at which arginolysis was reduce ... | 1988 | 2843090 |
| differential modulation of adherence of oral streptococci by human neutrophil myeloperoxidase. | in this study, the modulation of adherence of hydrogen peroxide (h2o2)-producing and non-h2o2-producing strains of oral streptococci by the host leukocyte enzyme myeloperoxidase (mpo) was examined. it was found that exposure to mpo decreased adherence of many strains of oral streptococci to saliva-coated hydroxyapatite beads in the presence of exogenous h2o2 and chloride. the mpo-h2o2-cl-system increased the adherence of one strain. in the absence of exogenous h2o2, the mpo-h2o2-cl-system decrea ... | 1988 | 2844867 |
| inhibition of adhesion of viridans streptococci to fibronectin-coated hydroxyapatite beads by lipoteichoic acid. | fibronectin-coated hydroxyapatite (fnha) beads were used in a model adhesion assay to isolate the lipoteichoic acid (lta) mediated adhesion of oral streptococci. representative strains of the commonly isolated viridans streptococci were incubated with fnha beads in the presence and absence of exogenous lta. the lta inhibited the adhesion of all strains to a greater or lesser extent, but only a very few strains were inhibited by more than 90%. strains of streptococcus sanguis type ii and streptoc ... | 1988 | 2854117 |
| [contemporary aspects on aphthous ulcers]. | | 1987 | 2856428 |
| surface properties of streptococcus sanguis fw213 mutants nonadherent to saliva-coated hydroxyapatite. | seventeen mutants of streptococcus sanguis fw213 nonadherent to saliva-coated spheroidal hydroxyapatite were isolated after mutagenesis with ethyl methanesulfonate, nitrosoguanidine, nitrous acid, hydroxylamine, or 2-aminopurine. enrichment for nonadherent mutants was accomplished by successive adsorptions of the adherent strains to saliva-coated hydroxyapatite. after enrichment, variant colonial morphology on tryptic agar was used as a screening technique for selection of nonadherent mutants, w ... | 1985 | 2857684 |
| antibodies that bind to fimbriae block adhesion of streptococcus sanguis to saliva-coated hydroxyapatite. | antibodies raised against a fimbriated, adhesive strain of streptococcus sanguis (fw213) were found to block the adhesion of this organism to saliva-coated hydroxyapatite. antibodies were made specific for adhesion antigens by adsorption with isogenic, nonadhesive mutants (for rabbit polyclonal adsorbed antibody) or selection based on nonreactivity with two nonadhesive mutants (for monoclonal antibody). rabbit antibody raised against isogenic, nonfimbriated nonadhesive mutants served as a contro ... | 1985 | 2860066 |
| genetic transformation in streptococcus sanguis. | | 1985 | 2864407 |