| definition of two ld antigens in rhesus monkeys. | two rhesus (macaca mulatta) monkey lymphocyte-defined (ld) antigens have been identified using two typing cells as stiumlators in a one-way mixed leukocyte culture (mlc) assay. an analysis of the genetic behavior of these ld antigens in six rhesus monkey families revealed that both antigens were linked with rhla. one probable recombinant indicated that the ld locus lies outside the two known rhla-sd loci and the locus which controls the serum protein, properdin b(bf). these two antigens, ld1 and ... | 1979 | 87026 |
| hemolytic plaque formation to allogeneic human type a and b red cells. | plaque-forming cells can be generated from peripheral blood lymphocytes provided that the lymphocytes are prestimulated by in vitro sensitization in the presence of pokeweed mitogen. of 91 blood type o persons whose lymphocytes were stimulated with red cell a antigen, 52% responded with plaque formation, whereas of 40 type o persons whose lymphocytes were stimulated with red cell b antigen, only 20% produced plaques. similarly, reciprocal stimulation of type a and b persons showed higher plaque ... | 1979 | 87029 |
| study of interaction between igg and igm antibodies against rubella virus by the immunofluorescence method. | in immunoglobulin fractions or after elimination of igg by absorption the immunofluorescence test for rubella igm antibodies is more sensitive than in whole serum. blocking of igm activity by igg antibodies was eliminated when the time of incubation of the serum with virus antigen was prolonged. after prolonged incubation higher titres of rubella antibodies were also obtained in the igm immunoglobulin fractions. protein a in staphylococcus aureus suspension effectively absorbs antibodies of igg ... | 1979 | 87117 |
| leucocyte migration inhibition test as an index of immunological response to measles virus. iv. blood, spleen, lymph nodes and peritoneal exudate cell reaction. | the migration inhibition test of leucocytes isolated both from the peripheral blood, spleen and lymph nodes as well as peritoneal exudate cells of guinea pigs, immunized with the various doses of measles virus was determined. for in vitro testing of cellular immunity to measles virus, viral antigens could be used in both infective and inactivated form. | 1979 | 87118 |
| study on dissimilatory reduction of sulphates. | a new strain of sulphate reducing bacteria was isolated from swampy forest soil. after 120 h reduction, sulphate conversion attained 100%, the molar ratio of the consumed lactate and reduced sulphate amounted to 2:1. this confirms the reduction mechanism proposed by senez (1951). | 1979 | 87119 |
| [analysis of the circadian rhythm of motor activity in white rats by using several pharmacologic agents which act on cerebral monoamines]. | three pharmacological agents, (disulfiram, imipramine and reserpine) influencing the brain monoamine transmitters have been studied to explain the mechanism involved in the motor circadian rhythm. the new results corroborate our previous ones : the norepinephrine transmitter responsible for the paradoxical sleep is unable to explain the depressed metabolic phase of the circadian rhythm; the negative results obtained with disulfiram and imipramine corroborate the previous results with nialamide. ... | 1978 | 87162 |
| [reserpine inhibition of the hypotensive and permeability-promoting effect of o-hydroxyethyl derivatives of rutoside]. | | 1978 | 87166 |
| [convulsant properties of methyseleno-2-benzoic acid in rats]. | | 1978 | 87170 |
| reinforcing stimulation of the postero-lateral hypothalamus and mating behaviour in the rat. | male rats implanted with chronic electrodes into the postero-lateral hypothalamic site were tested for self-stimulation behaviour. rats exhibiting steady self-stimulation behaviour were observed during mating tests with an oestrus female. during these tests the hypothalamus of male rats was stimulated. results show that no stimulus-bound sexual behaviour was observed. nevertheless, correlations were found between the rewarding value of the cerebral stimulation and the specific sexual components ... | 1978 | 87181 |
| [therapy and prophylaxis of experimental staphylococcal nephritis of the rabbit with gamma-globulin and f(ab')2-fragments (author's transl)]. | prophylactic and therapeutic effects of immunoglobulins and their combination with ampicillin were studied in an experimental bacterial nephritis model after i.v. injection of staphylococcus aureus into rabbits. untreated human igg and a preparation containing f(ab')2-pieces were investigated on therapeutic effects. both antibody preparations prevented the lethal purulent inflammation of kidneys when injected immediately before the infection. the course of the pathological process after infectio ... | 1979 | 87206 |
| projections on the cortical somatic i barrel subfield from ipsilateral vibrissae in adult rodents. | in adult rats and mice, ipsilateral projections to si posteromedial barrel subfield (pmbsf) are demonstrated for mystacial vibrissae (sinus hairs). mechanical stimulation with angular deflections in the 2-5 degrees range, elicits potentials in limited areas of si whose geometric centers coincide for two homologous (contra- and ipsilateral) vibrissae. the cortical domains for two adjacent vibrissae overlap one another slightly. phase reversal of potentials and unit discharges are also present wit ... | 1979 | 87318 |
| relationship between histocompatibility antigens, other surface determinants and the ige receptor on rat mast cells. | rat alloantibodies recognizing classical transplantation antigens (cta) or non-h-1 determinants were able to compete effectively with monomeric ige or igg-coated sheep erythrocytes for receptor sites on the rat mast cell surface. inhibitory capacity, however, was entirely confined to anti-cta antibodies of the igg2a subclass, whereas igg1 antibodies lacked this ability. analogously, f(ab')2 fragments of anti-cta antibody consistently failed to affect ige binding, but exposure of cell-bound f(ab' ... | 1979 | 87327 |
| similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice. | amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ... | 1979 | 87374 |
| the effect of hapten-specific suppression of ige on antigen-induced histamine release from mouse peritoneal mast cells. | subcutaneous injections of a mixture of dinitrophenylated ovalbumin (dnp3-oa) and dextran sulfate into swiss-webster mice elicited short-lived primary and long-lasting secondary ige antibody responses to both dnp and oa. histamine was released on in vitro challenge with antigen (oa or dnp22-bsa) of washed peritoneal mast cells (pmc) obtained from mice during a primary or a secondary ige response. administration of an intravenous injection of a tolerogenic conjugate of dnp8-mouse gamma-globulin, ... | 1979 | 87380 |
| mode of action of a 5-nitrofuran drug (sq 18506) against trypanosoma cruzi. | | 1979 | 87384 |
| association of carbon-13 enriched human encephalitogenic nonapeptide with a membrane surface. | | 1979 | 87395 |
| intracellular marking with lucifer yellow ch and horseradish peroxidase of cells electrophysiologically characterized as glia in the cerebral cortex of the cat. | intracellular microelectrodes filled with either lucifer yellow ch, a highly florescent dye, or horseradish peroxidase (hrp) were used to electrophysiologically characterize and mark cells in the cerebral cortex of cat. fifty-eight cells, characterized electrophysiologically as glia, were marked with lucifer yellow ch. all were identified as protoplasmic astrocytes, and included cells in the glia limitans of the molecular layer. an additional 54 cells, similarly characterized as glia, were label ... | 1979 | 87405 |
| solid phase radioimmunoassay for quantitation of antigen-specific igg in human sera with 125i-protein a from staphylococcus aureus. | radiolabeled protein a from staphylococcus aureus (staph a) has been used to develop a solid phase, noncompetitive radioimmunoassay for quantitation of specific igg antibody. the assay involves two incubations: first, agarose-insolubilized antigen is mixed with serum samples for 1 to 4 hr during which specific antibody is bound; second, after a washing procedure, the solid phase immune complexes are incubated for 4 to 18 hr with 125i-staph a, during which the radiolabeled detection protein binds ... | 1979 | 87416 |
| the expression of ia antigenic determinants on macrophages required for the in vitro antibody response. | a subpopulation of antigen-presenting macrophages required for an in vitro antibody response to burro erythrocytes was deleted by pretreating the splenic macrophages with anti-ia serum and complement (c). the in vitro response of the macrophage depleted t-b cell population could not be restored by the addition of macrophages resistant to anti-ia antibodies and c (ia-). the response of ia- macrophages and the macrophage-depleted t-b cells was only reconstituted by the addition of ia+ macrophages. ... | 1979 | 87431 |
| immune suppression in vivo with antigen-modified syngeneic cells. ii. t cell-mediated nonresponsiveness to fowl gamma-globulin. | intravenous administration of syngeneic spleen cells coupled with the palmitoyl derivative of fowl gammma-globulin (p-f gamma g) results in a profound state of f gamma g-specific tolerance in c57bl/6 mice. administration of p-f gamma g coupled syngeneic cells specifically reduces both the primary and secondary hapten and carrier-specific pfc responses to tnp-f gamma g. since the haptenic response is affected, the tolerance functions at the level of the f gamma g-specific helper t cell. as few as ... | 1979 | 87447 |
| maturation of the lymphoid system. i. induction of tolerance in neonates with a t-dependent antigen that is an obligate immunogen in adults. | a/j mice displayed a striking ontogenetic difference in the capacity to respond to dnp-ficoll, a t-independent antigen, and to aggregated human gamma-globulin (ahgg), a t-dependent antigen. thus, whereas responses to dnp-ficoll of 4-day-old mice were similar in magnitude to those of adult animals, responses to ahgg did not become pronounced until mice were some 30 to 40 days of age. the inability of young animals to respond to ahgg was reflective of a negative consequence of lymphocyte/antigen i ... | 1979 | 87468 |
| a histological and histochemical study of changes of fiber types in experimental myotonia. | in both fast and slow muscles of rats treated with 20--25 diazacholesterol there were qualitative alterations, such as changes of fiber outlines, numerous moth-eaten fibers and rare ring fibers. in addition there were generally larger groups of type i and intermediate fibers than in normal controls ("type-grouping" tendency) in the preparations for oxidative enzymes in the extensor digitorum longus (edl) of myotonic animals. quantitative evaluations of edl and soleus of myotonic rats revealed mo ... | 1979 | 87497 |
| somatosensory evoked potentials following stimulation of the trigeminal nerve in man. | somatosensory evoked potentials following trigeminal nerve stimulation can regularly be recorded from the contralateral scalp on c5/c6 (10--20 system), a region which overlies the primary face region of the somatosensory cortex. from the first three peaks analyzed (n 13, p 19 and n 26), the first positive peak (p 19) is most prominent and reliable and therefore is recommended for the routine measurements of neurophysiological examination. | 1979 | 87502 |
| the effect of full-thickness skin grafts on the actomyosin content of contracting wounds. | in evaluating the myofibroblast theory of wound contraction, comparisons were made between grafted and nongrafted 15-day wounds. pregnant rabbit uteri and normal rabbit skin were used as controls. small amounts of actin and myosin were present in contracting open wounds but not in noncontracting grafted wounds, which suggests some participation for contractile proteins in wound contraction. the current study refutes the widely accepted contention that wound granulation tissue is as rich as smoot ... | 1979 | 87511 |
| reticuloendotheliosis virus: detection of immunological relationship to mammalian type c retroviruses. | reticuloendotheliosis virus (rev) p30 shares cross-reactive determinants and a common nh2-terminal tripeptide with mammalian type c viral p30's. an interspecies competition radioimmunoassay was developed, using iodinated rev p30 and a broadly reactive antiserum to mammalian virus p30's. the avian leukosis-sarcoma viruses and mammalian non-type c retroviruses did not compete in this assay. previous data indicating that the rev group is not represented completely in normal avian cell dna lead us t ... | 1979 | 87519 |
| limited immunodeficiency. | | 1978 | 87559 |
| breast-feeding and smoking. | | 1979 | 87639 |
| cefotaxime for bacterial meningitis. | | 1979 | 87714 |
| sputum-smear-negative pulmonary tuberculosis: controlled trial of 3-month and 2-month regimens of chemotherapy. | of 1072 chinese patients with radiographically active pulmonary tuberculosis and no microscopic evidence of acid-fast bacilli in sputum examinations, only 691 (64%) were sputum-culture negative. all patients were randomly allocated to selective chemotherapy (antituberculosis chemotherapy not being started until the activity of the disease had been confirmed), to daily streptomycin, isoniazid, rifampicin, and pyrazinamide for 2 months or 3 months, or to a standard 12-month control regimen. during ... | 1979 | 87829 |
| inhibition of reverse transcriptase activity by benzophenanthridine alkaloids. | benzophenanthridine alkaloids, fagaronine 4, o-methylfagaronine 5,nitidine 1, allonitidine 3 and methoxydihydronitidine 2 have been shown to possess inhibitory activity against reverse transcriptase of rna tumor viruses. the enzyme inhibition (50%) by these alkaloids was found in the range of 6-60 microgram per milliliter of the reaction mixture when polynucleotide-oligodeoxynucleotide complexes were used as template primers. the results suggested that the benzophenanthridine alkaloids interacte ... | 1979 | 88001 |
| effect of double infection of cowpox virus-infected cells with paramyxovirus (sendai virus) on formation of cowpox virus-specific cell surface antigen. | the formation of cowpox virus-specific cell surface antigen (cpv s-ag) was significantly enhanced by double infection with hvj (sendai virus). simultaneous double infection, superinfection with hvj and superinfection with cpv of cells persistently infected with hvj similarly enhanced the formation of cpv s-ag, while pre-infection with hvj was ineffective. to be effective, cells must be infected at a m.o.i. of greater than or equal to 1.0 and hvj gene functions had to be expressed. the hvj-infect ... | 1978 | 88002 |
| [mineral oil emulsion of the gamma isomer of hcch in sheep psoroptic mange]. | | 1979 | 88121 |
| characterization of an amphotropic murine c-type virus that is nb-tropic. | | 1979 | 88128 |
| [effect of antiglial and antineuronal immunoglobulins on the processes of retention and reproduction of a reinforced habit]. | | 1979 | 88137 |
| free flap in an experiment - a histological view. | | 1978 | 88142 |
| human corneal endothelium in organ culture. the influence of temperature and medium of incubation. | forty-four human corneas from patients between 21 and 86 years were incubated in eagle's minimum essential medium with earle's salts 10--46 h post mortem. the influence of incubation temperature and composition of the medium on endothelial survival was evaluated. whole corneas were stained by alizarine red. recent cell loss was indicated by morphological alterations in the endothelial pattern. after 20--28 h of incubation minimum cell loss was found at 31 degrees c when 8% dextrane-250 and 20% s ... | 1979 | 88158 |
| anaphylatoxin-induced shock and two patterns of anaphylactic shock: hemodynamics and mediators. | in the dog, different cardiorespiratory reactions were identified in two types of anaphylactic shock and in c5a-at (anaphylatoxin)-induced shock. all three types had in common a portal blood pooling with consequent decrease in the venous return, cardiac output, and arterial pressure. in anaphylaxis (a) of the first type, at a low titer of hemagglutinating antibodies, the latent period was 68 s and heart and lung function was unchanged. in the second type, at high titer, the latency was 19 s and ... | 1979 | 88165 |
| binding of antigen to surface ig during in vitro hyposensitization of isolated rat mast cells. | the mechanism of in vitro hyposensitization was examined in isolated rat mast cells. surface distribution of immunoglobulin was examined by immunofluorescence technique. hyposensitization could be ascribed neither to changes in the distribution of surface immunoglobulins nor to capping or shedding phenomena. the distribution of cell-bound antigen and the number of antigen-positive cells (patch-bearing cells) was not changed by hyposensitization. the binding of antigen to surface ig was quantitat ... | 1979 | 88188 |
| denaturization of allergen p: effect on allergenicity, antigenicity and immunogenicity. | when allergen p was denatured by 8m urea, the modified molecule still reacted with ige specific for the native allergen but not with hemagglutinating antibodies. heating at 100 degrees c abolished the reaction in both cases. the results suggested differences between allergenic and antigenic capacities which may be based on structural differences of the antigenic determinants. | 1979 | 88195 |
| effects of passively and actively acquired antibody on bovine campylobacteriosis (vibriosis). | | 1979 | 88198 |
| types of striatonigral neurons labeled by retrograde transport of horseradish peroxidase. | | 1979 | 88205 |
| a comparative study of o'nyong nyong virus with chikungunya virus and plaque variants. | two plaque variants of chikungunya (chik) virus were serologically compared with o'nyong nyong (onn) virus in order to elucidate the reported one way antigenic relationships between the two viruses. three different hypotheses are examined and evidence is shown to support one of them. comparison of some biological properties of the viruses showed onn to be distinct in some respects. all viruses and variants were found to replicate in anopheles gambiae cells. | 1979 | 88212 |
| [simple method of detection of fc receptor sites on cells]. | a bacterial adhesion technique for identification of fc-receptor bearing cells is based on the ability of staphylococcus aureus, cowan 1 strain, to form rosettes with cells previously treated with immune complexes or heat aggregated ig g. the new technique is distinguished from other fc-receptor detection methods by its simplicity and ease of detection of both immune complexes and aggregated igg-binding cells. | 1979 | 88236 |
| [the antibacterial action on dental plaque of antibiotics used in dentistry. 2. in vitro experiments on streptococcus mutans, staphylococcus aureus and fusobacterium nucleatum (author's transl)]. | | 1977 | 88239 |
| expression of endodermally derived and neural crest-derived differentiation antigens by human lung and colon tumors. | | 1979 | 88254 |
| surface antigens of human glioma cells shared with normal adult and fetal brain. | | 1979 | 88260 |
| properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line. | two glycoproteins, gp-1 and gp-2, have been isolated from an extracellular membrane synthesized in cell culture by an embryonal carcinoma-derived cell line. the amino acid and carbohydrate compositions have been determined. both proteins are rich in half-cystine residues and contain approximately 12-15% carbohydrate. antibodies have been obtained against one of the glycoproteins, gp-2, in rabbits. the antibody reacts with basement membranes from adult mouse and human kidney glomeruli and tubules ... | 1979 | 88263 |
| immune responses to weakly immunogenic virally induced tumors. v. short in vitro cultivation of yac changes its antigenic properties. | | 1979 | 88269 |
| hepatic function assessed (in rats) during chemotherapy with some anti-cancer drugs. | using rats, we studied how best to assess hepatic damage after administering therapeutic doses of each of five anti-cancer drugs or of the hepatotoxin, carbon tetrachloride. as indexes, we compared measurement of the concentration of administered antipyrine in plasma with measurement in serum of alpha-fetoprotein or of the activities of five enzymes that reportedly best reflect hepatic damage. the biological half-life of antipyrine in the plasma was increased more than threefold on pretreating t ... | 1979 | 88282 |
| 4th international workshop on human gene mapping. report of the committee on the genetic constitution of the x and y chromosomes. | | 1978 | 88299 |
| the h-y antigen:production of antibodies, detection, and cross-reaction between mouse, rat, and human. | | 1978 | 88303 |
| t antigen banding on chromosomes of simian virus 40 infected muntjac cells. | chromosomes were prepared from mitotic munjac cells 48 to 72 h after infection with sv40 virus. when stained for sv40 t antigen by indirect immunofluorescence, all chromosomes within an infected cell were fluorescent, indicating the presence of t antigen. furthermore, the chromosomes were not uniformly stained but appeared to have regions of high and low fluorescence intensity. a variety of controls showed that the banding patterns are specific and highly reproducible and may indeed reflect the ... | 1979 | 88306 |
| palliation of massive hemoptysis from unresectable carcinoma of the lung. | unresectable carcinoma of the lung has traditionally been recognized as a contraindication to surgery in massive hemoptysis. a 60-year-old man had massive hemoptysis. at surgery an unresectable neoplastic mass invading the mediastinum and great vessels was encountered. subtotal resection was accomplished using a stapler (autostapler). the margins of the bronchial and vascular staples were of necessity placed directly through the tumor. the patient had an uneventful recovery and has survived six ... | 1979 | 88310 |
| changes in seizure susceptibility, sleep time and sleep spindles following thalamic and cerebellar lesions. | the present experiment attempted to clarify conflicting evidence on the relationship of sleep spindles to seizure activation. seizure thresholds were calculated in minutes post-injection following ip administration of the convulsant drug monomethylhydrazine (mmh) to cats with lesions intended to alter the occurrence of spontaneous 12-15 c/sec sleep spindles recorded from sensorimotor cortex. twelve cats with bilateral cortical and subcortical recording electrodes were divided into 3 groups recei ... | 1979 | 88323 |
| nocturnal and diurnal sleep in macaca sylvana. | in macaca sylvana restrained in chairs, continuous 24 h recordings were obtained of scalp eeg, neck emg, bilateral eog, motility of the head and lever manipulation. a total of 32 days were studied in 8 monkeys. recordings were divided into epochs of 30 sec and analyzed in a pdp-12 computer. four stages of sleep and 5 stages of wakefulness were identified by combination of the above data. results were as follows: (1) during the night, there was 21% wakefulness and during the day 20% sleep, includ ... | 1979 | 88327 |
| spinal evoked potential in man: a maturational study. | summated evoked potentials to peroneal nerve stimulation which arise in the cauda equina and spinal cord afferent pathways were recorded from surface electrodes placed over the spine of 95 infants, children and adults. the conduction velocity of these potentials from lumbar to cervical recording sites was calculated. additionally, segmental conduction velocities over peroneal nerve, caudal and rostral spinal cord were determined. in all age groups the speed of conduction up the spine was non-lin ... | 1979 | 88331 |
| automatic recognition of inter-ictal epileptic activity in prolonged eeg recordings. | a method of automatic recognition and quantification of inter-ictal epileptic activity in the human eeg had previously been developed and tested using short recordings from awake subjects. this paper describes the adaptation of the method for use during overnight recordings in free-moving unattended patients, in combination with the already existing seizure monitoring system. eeg s were recorded from scalp and sphenoidal electrodes, using cable telemetry and a pdp-12 computer. the spike and shar ... | 1979 | 88339 |
| vestibulo-ocular responses during the states of sleep in the cat. | the vestibulo-ocular response (vor) was recorded during natural sleep in cats with chronically implanted electrodes. by using a small amplitude sinusoidal head rotation (11 degrees) peak-to-peak at 0.4 hz) which elicited only slow compensatory eye movements, the vor amplitude was found to decrease steeply (down to 40% or less) during slow-wave sleep. the phase of the vor with respect to head position remained approximately constant. with a larger amplitude of sinusoidal rotation (320 degrees pea ... | 1979 | 88340 |
| differential arousal response to gustatory stimuli in the awake rabbit. | ecog arousal response as elicited by deionized water and by several concentrations of sweet, salty, sour and bitter tasting substances, all applied intraorally, were studied in the awake restrained rabbit. the study was carried out on 17 chronic preparations of adult male animals (2.6 kg average body weight). water as a stimulant was presented in 60 trials and tastants across qualities and concentrations in 245 trials. arousal was quantitatively characterized by its duration, frequency increment ... | 1979 | 88352 |
| individual differences in the human electroencephalogram during quiet wakefulness. | | 1979 | 88364 |
| kinetic complexity of nuclear poly(a)-containing rna in normal and regenerating rat liver. | poly(a)-containing rna isolated from liver nuclei of untreated rats and 3 h or 12 h after partial hepatectomy or sham operation was hybridized to the complementary dnas (cdnas). in the homologous reactions two major components could be seen. when compared to normal liver, the complexity of the least abundant class was lower in nuclei from livers 3 h after partial hepatectomy and was higher in those isolated 12 h after operation. the heterologous reactions revealed an increase of some abundant p ... | 1979 | 88367 |
| relative effectiveness of spinal cord and purified myelin basic protein in producing resistance to experimental allergic encephalomyelitis. | isolated myelin basic protein (mbp) was less effective than an equivalent amount of spinal cord in inducing protection against experimental allergic encephalomyelitis produced by a challenge of either cord, purified myelin or mbp. complete protection was only obtained when an mbp challenge was preceded by spinal cord treatment. there was a 100% incidence of disease in the guinea pigs pretreated with mbp before challenge with spinal cord or myelin, but the onset was delayed by 3--4 weeks and the ... | 1979 | 88369 |
| leishmania mexicana and leishmania tropica: cross immunity in c57bl/6 mice. | | 1979 | 88370 |
| proton relaxation enhancement to probe the effects of antigen binding on pig antibody structure. | | 1979 | 88375 |
| renal cortex sialyltransferase activity upon tamm-horsfall glycoprotein, fetuin and rat glomerular basement membrane. | | 1979 | 88378 |
| [spontaneous rosette forming, fc and complement receptor bearing lymphocytes in pediatric diseases]. | investigations with lymphocyte subpopulations were made in 51 children aged from 1-14 years with infections of the upper airways, with acute leukaemias, and other malignant diseases. t-lymphocytes were registered by means of the spontaneous rosette test. the attempt of proving b-lymphocytes was made by means of an eac test with human erythrocytes, anti-d-immunoglobulins and human complement. the results were compared with those obtained by an eac test with sheep erythrocytes, sheep haemolysin an ... | 1979 | 88386 |
| cytochemical analysis on a case of familial 17ps. | chromosome 17ps was identified in the mother and daughter but not the father of a normal family with no history of congenital abnormality. in addition to g-band and ag-nor staining, previously used to study this abnormality, we applied n-band and c-band techniques. our results showed that 17ps has no demonstrable ribosomal cistron or constitutive heterochromatin. | 1979 | 88409 |
| localization of the nucleolar organizer by computer-aided analysis of a variant no. 21 in a human isolate. | a variant chromosome no. 21 consisting of two stalks and two satellites in tandem was detected during a survey of a human isolate. the variant segregated in three generations of a large kindred. one male had the variant no. 21, a metacentric y, and a 47,xxy complement; however, no other evidence of chromosomal nondisjunction was found. computer-aided analysis of sequentially stained variant no. 21 chromosomes indicated that silver-stained material corresponded to the proximal stalk region (as de ... | 1979 | 88411 |
| effect of norepinephrine on in vitro histamine release from rat mast cells. | the effect of norepinephrine on compound 48/80-induced histamine release from rat peritoneal mast cells, was studied in an in vitro system. it was found that norepinephrine, within the concentration range 10(-5)--10(-3) m, exerts a significant, dose-related, repressive effect. this effect is greatly potentiated by the beta-antagonist practolol (10(-3) m), throughout the concentration range of 10(-11)--10(-3) m norepinephrine. methoxyamine, a selective alpha-adrenergic agonist, also represses his ... | 1979 | 88419 |
| the inhibitory effect of xanthine derivatives on alkaline phosphatase in the rat brain. | histochemical and biochemical studies were carried out on the inhibition of alkaline phosphatase (a1-p) activity in rat cerebral cortex with various methylxanthine derivatives. the histochemical study revealed that a1-p activity was completely inhibited with 2 mm theophylline or aminophylline, only slightly inhibited with 5 mm of xanthine, and no way inhibited even with 5 mm of diprophylline or caffeine. the biochemical study showed that a1-p activity was markedly inhibited by 1 mm theophylline, ... | 1979 | 88432 |
| a new selective ultrahistochemical method for the demonstration of calcium using n,n-naphthaloylhydroxylamine. | a new, simple, rapid, and highly sensitive and selective method for the ultrahistochemical detection of calcium is described. the reagent n,n-naphthaloylhydroxylamine (1,8-c10h6con(ona)co) sodium salt was employed in this study for the demonstration of calcium at the subcellular level in relaxed and contracted muscles (smooth muscle of the stomach, thoracic aorta, and myocardial muscle cells) of the rat (in vitro as well as in vivo) and in the human vascular smooth muscle of the aorta with ather ... | 1979 | 88434 |
| steroid receptor content in cytosol from normal and hyperplastic human prostates. | analyses of steroid hormone receptors were performed using a dextran-coated charcoal technique in cytosolic preparations from 40 cases of benign prostatic hyperplasia (bph) and 10 normal human prostates. binding data were calculated according to scathchard. in all bph specimens, receptors for the synthetic androgen methyltrienolone (mt) were found (mean maximum number of binding sites, 566 fmol/mg dna; mean kd, 0.61 nm), and 25 of 28 samples contained progestin [17 alpha, 21-dimethyl-19-nor-4.8- ... | 1979 | 88456 |
| antigenic determinants of influenza virus hemagglutinin. iii. competitive binding of antibodies directed against "common" and "strain-specific" antigenic determinants of a/memphis/72 hemagglutinin. | | 1979 | 88484 |
| release of prolactin and lh and histamine-containing cells in brain. | the effects of histamine released from mast cells by the compound 48/80, a degranulating agent, on plasma prolactin and lh levels, were examined in rats. compound 48/80 given systemically (s.c.) at a dose of 2 mg/100 g b.w. significantly increased at 15 and 60 min after injection, both prolactin and lh release in ovariectomized, steroid-primed rats. prolactin but not lh was also augmented in the plasma of male rats following s.c. administration of compound 48/80. there were no modifications of p ... | 1979 | 88502 |
| specific binding of prolactin by the prostate gland of the rat and man. | specific binding sites for 125iodine-prolactin are present in membrane particles obtained from the rat ventral prostate, human benign prostatic hyperplasia and prostatic adenocarcinoma. in the ventral prostate glands of young rats (1 to 4 months old) specific binding of 125iodine-prolactin is higher than in older animals (greater than 8 months old). subcellular distribution studies revealed that specific 125iodine-prolactin binding activity is associated primarily with the 15,000 and 100,000 g p ... | 1979 | 88529 |
| immunospecificity of nuclear nonhistone protein-dna complexes in colon adenocarcinoma. | tumor-specific antisera against dehistonized chromatin isolated from transplantable colon adenocarcinoma (from male noninbred sprague-dawley rats) were produced. the specificities of these antisera were determined by complement fixation. in the presence of these antisera, only chromatin from colon adenocarcinoma significantly fixed complement, whereas chromatins isolated from normal rat colon epithelia were inactive. administration of 1,2-dimethylhydrazine to rats produced an early change in the ... | 1979 | 88536 |
| oncofetal antigen: a tumor-associated fetal antigen immunogenic in man. | oncofetal antigen (ofa) has been defined with the use of human natural antibodies as a membrane antigen of human cancer cells that cross-reacts with human fetal brain tissues. the immunogen that elicits the antibody is unknown. the present study was undertaken to examine the immunogenicity of the ofa found on tumor cells. postoperative melanoma patients were immunized with ofa-positive melanoma cells. anti-ofa reactivities in the immunized sera were titrated by the immune adherence assay with th ... | 1979 | 88539 |
| effect of age on axoplasmic transport of cholinesterase in rat sciatic nerves. | senile muscle atrophy has been attributed to an impaired ability of old nerves to transport trophic factors. to evaluate the effect of age on axoplasmic transport, we measured the accumulation of cholinesterase activity above a ligature around sciatic nerves of young (7--8 months), middle-aged (19--20 months), and old (31--32 months) male rats. protein content and cholinesterase activity per mm of nerve were higher in middle-aged and old than in young nerves. however, accumulation of cholinester ... | 1979 | 88552 |
| meningitis in the elderly. | | 1979 | 88602 |
| passive transfer in diabetes mellitus. | | 1979 | 88629 |
| legionnaires' disease caused by legionella pneumophila serogroup 3. | | 1979 | 88631 |
| [immunosuppression (author's transl)]. | among different procedures of immunosuppression in man medical treatment with cytotoxic drugs has become the most important. biological methods are still under development but promise to gain in importance since they are more specific and have fewer side effects. the clinical effect of immunosuppressive therapy in organ transplantation is undisputed. its effects has been proven in some but not all immuno-inflammatory diseases. before immunosuppressive therapy is started it must be known whether ... | 1979 | 88671 |
| electron microscopic identification of mammillary body terminals in the rat's av thalamic nucleus by means of anterograde transport of hrp. a quantitative comparison with the em degeneration and em autoradiographic techniques. | a comparison has been made between the number of horseradish peroxidase (hrp)-labeled mammillary body terminals in the antero-ventral (av) nucleus and the number of such terminals labeled with the electron microscopic (em) autoradiographic [6] and em degeneration technique [6]. with the antero-grade hrp technique less terminals were labeled than with the autoradiographic technique, but more than with the degeneration technique. furthermore it may be concluded that the anterograde hrp technique i ... | 1979 | 88693 |
| retrograde transport of bisbenzimide and propidium iodide through axons to their parent cell bodies. | two fluorescent substances bisbenzimide (bb), which fluoresces yellow-green and propidium iodide (pi), which fluoresces orange were found to be transported retrogradely through axons to their parent cell bodies in rat and cat. bb gives a very strong and long lasting fluorescent retrograde neuronal labeling and is very effectively transported over long distances both in rat and cat. bb and pi also label glial nuclei around retrogradely labeled neurons. bb in addition labels glial nuclei along axo ... | 1979 | 88694 |
| fuorescent retrograde neuronal labeling in rat by means of substances binding specifically to adenine-thymine rich dna. | six fluorescent substances, binding specifically to adenine-thymine rich dna, were injected in rat caudate-putamen. this resulted in retrograde axonal transport and fluorescent retrograde labeling of neurons in center-medium parafascicular nucleus, substantia nigra and dorsal raphe. two of these substances, i.e. "true blue' and 'granular blue', give a very striking blue fluorescent retrograde neuronal labeling. mid-thoracic spinal injections of these two substances in rat also resulted in a pron ... | 1979 | 88697 |
| the influenza virus: antigenic composition and immune response. | the architecture and chemical composition of the influenza virus particle is described with particular reference to the protein constituents and their genetic control. the dominant role in infection of the surface proteins - haemagglutinins and neuraminidases - acting as antigens and undergoing variation in time known as antigenic drift and shift is explained. the immuno-diffusion technique has illuminated the interrelationships of the haemagglutinins of influenza a viruses recovered over long p ... | 1979 | 88726 |
| the antiherpesvirus action of phosphonoacetate. | | 1979 | 88744 |
| [experimental inductions of tissue alterations in the lung following bleomycin and irradiation exposure (author's transl)]. | | 1978 | 88746 |
| immunofluorescent staining of platelet suspensions and detection of antiplatelet antibody in patients with idiopathic thrombocytopenic purpura. | direct immunofluorescent staining of 31 specimens of platelets obtained from 13 cases of idiopathic thrombocytopenic purpura (itp) revealed positive staining on the surface of platelets for both immunoglobulins (igs) and human b1c globulin in 9 specimens, for only igs in 1 specimen and for human b1c alone in 5 specimens. the pattern of the positive immunofluorescent staining was granular. indirect immunofluorescent staining of normal platelets in serum obtained from patients with itp was positiv ... | 1979 | 88810 |
| release of heparin and histamine from guinea pig mast cells [proceedings]. | | 1979 | 88887 |
| the influence of cyclic nucleotides on histamine release from rat peritoneal mast cells [proceedings]. | | 1979 | 88893 |
| dansylcadaverine specific staining for transamidating enzymes. | | 1979 | 88908 |
| a new assay of estrogen receptor by thin-layer gel filtration. | | 1979 | 88911 |
| alterations in distribution of sudanophilic lesions in rabbits after cessation of a cholesterol-rich diet. | | 1979 | 88945 |
| enzymic polyadenylation of 5s ribosomal ribonucleic acid and synthesis of a complementary deoxyribonucleic acid. | the 5s ribosomal rna has been isolated, pure and intact, from rat liver (5 mg of 5s rna from 150g of liver). the 5s rna serves as a primer for calf thymus poly(a) polymerase with 20% of the efficiency of (ap)3a. bacterial 5s rna and transfer rna also serve as primers; rat liver 18s and 28s ribosomal rnas support poly(a) synthesis poorly. neither the 5s rna primer nor the appended poly(a) tract is nicked or degraded by poly(a) polymerase, and initiation of poly(a) tracts on 5s rna primers continu ... | 1979 | 88957 |
| [compartmentation of gramicidin 5 in membranes of sensitive bacteria and protein-lipid interactions]. | gramicidin s is sorbed on the isolated membranes of granicidin-sensitive micrococcus lysodeikticus strain. the antibiotic inhibits the membrane malate dehydrogenase within the temperature range of 9--42 degrees c, i.e. under conditions of gel and liquid-crystalline lipid state; however its effect at 10 degrees c is 10 times as low as is observed at 42 degrees c. the inhibitory effect of gramicidin s on malate dehydrogenase can be eliminated and the antibiotic can be removed from the membrane by ... | 1979 | 88965 |
| [heterogeneity in organization of lipids in the bacterial membrane]. | the lipids of micrococcus lysodeikticus membranes were 50%-substituted by phosphatidyl choline using lipid-exchanging proteins isolated from rat liver. the incorporation of phosphatidyl choline into the membrane did not significantly change the malate dehydrogenase activity and the temperature dependence activity in the arrhenius plots for the enzyme. gramicidin s--modifier of membrane lipids--had similar effects both on the intact membranes and on the phosphatidyl-enriched membranes. a conclusi ... | 1979 | 88966 |
| autonomic block, cardiovascular depression and histamine release produced by polymyxin b in the cat. | the present study quantifies the autonomic block, the cardiovascular depression and the histamine releasing effects of polymyxin b in nine anaesthetized cats. the dose requirements for 50 per cent depression of the mean arterial blood pressure, the bradycardiac response to vagal stimulation, and the contraction of the nictitating membrane elicited by pre-ganglionic and post-ganglionic stimulation of the cervical sympathetic trunk have a narrow range of scatter, being of the order of 6--12 mg.kg- ... | 1979 | 89005 |
| the pathophysiology of asthma. | because postmortem studies of humans provide little information on the initial pathophysiologic events in asthma, animal models have been developed. recently the ascaris-allergic rhesus monkey has provided an opportunity to examine the onset of pathophysiologic changes following challenge and to correlate them with airway structure. these studies have suggested that the initial interaction between antigen and mast cells may occur in the bronchial lumen or in the epithelium superficial to the tig ... | 1979 | 89006 |
| reaction of cells from early chick embryos with antiserum against mouse sperm cells. | | 1979 | 89009 |