| the synthesis of phosphoendolpyruvate from pyruvate and atp by extracts of photosynthetic bacteria. | | 1966 | 5911083 |
| thiosulphate metabolism and rhodanese in chromatium sp. strain d. | | 1966 | 5915368 |
| the role of tetrathionate in the oxidation of thiosulphate by chromatium sp. strain d. | | 1966 | 5915369 |
| cobalt-free corrinoid compounds from photosynthetic bacteria. | | 1966 | 5927395 |
| kinetic analysis of the reaction of cytochrome cc' with carbon monoxide. | | 1966 | 5946624 |
| occurrence of the adenosine monophosphate inhibition of carbon dioxide fixation in photosynthetic and chemosynthetic autotrophs. | | 1966 | 5954698 |
| properties of chromatium subchromatophore particles obtained by treatment with triton x-100. | | 1966 | 5959463 |
| repetitions in the polypeptide sequence of cytochromes. | | 1966 | 5960866 |
| analyses of light-induced bacteriochlorophyll absorbance change and fluorescence emission in purple bacteria. | | 1966 | 5961107 |
| electron microscopy of ultrathin sections of micrococcus cryophilus. | | 1966 | 5962582 |
| early chemical events in photosynthesis: kinetics of oxidation of cytochromes of types c or f in cells, chloroplasts, and chromatophores. | | 1966 | 5966902 |
| absorption characteristics of bacteriochlorophyll types in purple bacteria and efficiency of energy transfer between them. | | 1966 | 5966925 |
| cytochrome photooxidation at liqud nitrogen temperatures in photosynthetic bacteria. | | 1969 | 5822417 |
| low potential titration of the fluorescence yield changes in photosynthetic bacteria. | | 1969 | 5822422 |
| [fixation of molecular nitrogen in relation to the electron-donor system of respiration and to photosynthesis]. | | 1966 | 5979910 |
| [effect of inhibitors on electron transport systems in purple bacteria with special reference to nitrogen fixation]. | | 1966 | 6002776 |
| changes in adenine nucleotides of intact chromatium d produced by illumination. | the total adenine nucleotide content of suspensions of chromatium d averaged 14 nmoles/mg of dry weight. of this, one-third to one-half was adenosine triphosphate (atp), even in suspensions incubated in darkness. illumination with high intensities caused a rise in atp and a drop mainly in adenosine diphosphate, the new steady state being reached in 5 to 15 sec at room temperature. the dark steady state was re-established 15 to 30 sec after returning the suspensions to darkness. the rates of thes ... | 1967 | 6025442 |
| biosynthesis of phenylalanine from phenylacetate by chromatium and rhodospirillum rubrum. | cultures of chromatium strain d and rhodospirillum rubrum incorporated (14)c from phenylacetate-1-(14)c during anaerobic growth. the radioactivity in the protein fraction of cells was mainly in phenylalanine. phenylalanine from chromatium cells grown in phenylacetate-1-(14)c was labeled at carbon 2. incorporation of phenylacetate by chromatium was decreased in the presence of exogenous phenylalanine, and de novo synthesis of phenylalanine from bicarbonate was less in medium containing either phe ... | 1967 | 6032506 |
| chemical characterization of high potential iron proteins from chromatium and rhodopseudomonas gelatinosa. | | 1967 | 6048308 |
| thermodynamics of light emission and free-energy storage in photosynthesis. | a planck law relationship between absorption and emission spectra is used to compute the fluorescence spectra of some photosynthetic systems from their absorption spectra. calculated luminescence spectra of purple bacteria agree well but not perfectly with published experimental spectra. application of the planck law relation to published activation spectra for systems i and ii of spinach chloroplasts permits independent calculation of the luminescence spectra of the two systems; if the luminesc ... | 1967 | 6048879 |
| electron tunnelling in cytochromes. | | 1967 | 6050223 |
| use of glass fiber filters for the rapid preparation of in vivo absorption spectra of photosynthetic bacteria. | | 1967 | 6051352 |
| molecular weight and amino acid composition of chromatium ferredoxin. | | 1967 | 6055172 |
| an electrophoretic study of the heme proteins of some photosynthetic bacteria. | | 1967 | 6058130 |
| orthorhombic sulphur formed by photosynthetic sulphur bacteria. | | 1967 | 6058316 |
| comparison between direct methods for determination of microbial cell volume: electron microscopy and electronic particle sizing. | size frequency distributions of different phototrophic and heterotrophic microorganisms were determined by means of scanning and transmission electron microscopy and electronic particle sizing. statistically significant differences existed among the three techniques used in this study. cells processed for electron microscopy showed lower mean cellular volumes than those processed for electronic particle sizing, reflecting a shrinkage by factors ranging from 1.1 to 6.2 (mean, 2.3). processing of ... | 1983 | 6347064 |
| cytochromes c' in their reaction with ethyl isocyanide. | the binding of ethyl isocyanide (eic) to a representative number of cytochromes c' is demonstrated. spectroscopic and equilibrium constants have been measured and compared for the binding of eic to cytochromes c' from the photosynthetic bacteria chromatium vinosum, rhodopseudomonas palustris, rhodospirillum rubrum, and rhodopseudomonas sphaeroides. while the absorption spectra of the eic complexes resemble those of eic complexes of other high-spin hemoproteins, the soret half band widths and ext ... | 1984 | 6087877 |
| [delayed bacteriochlorophyll luminescence and the primary stages of electron transport in photosynthetic reaction centers of purple bacteria]. | the results of studies of charge separation in photosynthetic reaction centers of purple bacteria are summarized. the findings concerning the sequence of initial steps of the electron transfer and properties of the electron carriers obtained by direct methods of differential optical absorption and esr spectroscopy are compared with the data on the bacteriochlorophyll delayed fluorescence resulting from reversal of charge separation. the data analysis gives an integrated description of the reacti ... | 1984 | 6095028 |
| the membrane-bound hydrogenase of alcaligenes eutrophus: ii. localization and immunological comparison with other hydrogenase systems. | immunological comparison of the soluble and the membrane-bound hydrogenase of alcaligenes eutrophus revealed no common antigenic determinants shared by the native proteins, however, a small amount of cross-reacting material was detected after freezing and thawing. immune precipitation assays supported previous observations indicating the membrane-bound hydrogenase to be localized in the outer surface of the cytoplasmic membrane. the membrane-bound hydrogenases of a. eutrophus and pseudomonas pse ... | 1980 | 6156647 |
| the role of soluble cytochrome c-551 in cyclic electron flow-driven active transport in chromatium vinosum. | | 1980 | 6243974 |
| interpretation of the mössbauer spectra of the high-potential iron protein from chromatium. | | 1980 | 6245869 |
| chromophore interactions in flavocytochrome c552: a resonance raman investigation. | resonance raman spectra with both soret and visible excitation have been obtained for chromatium flavocytochrome c552 and its isolated diheme subunit under varying conditions of ph and inhibitor binding. the spectra are generally consistent with previously established classification schemes for porphyrin ring vibrations. the presence of covalently bound flavin in the protein was apparent in the fluorescent background it produced and in flavin-mediated photoeffects observed in heme raman spectra ... | 1980 | 6246931 |
| effects of surface potential and membrane potential on the midpoint potential of cytochrome c-555 bound to the chromatophore membrane of chromatium vinosum. | the values of midpoint potential (em) of cytochrome c-555 bound to the chromatophore membranes of a photosynthetic bacterium chromatium vinosum was determined under various ph and salt conditions. after a long incubation at high ionic concentrations in the presence of carbonylcyanide m-chlorophenylhydrazone, which was added to abolish electrical potential difference between the inner and outer bulk phases of chromatophore, the em value was almost constant at ph values between 4.0 and 8.4. with t ... | 1980 | 6249347 |
| absorption bands of bacteriochlorophyll types in purple bacteria and their response to illumination. | | 1966 | 5971850 |
| near-infrared action spectra of fluorescence, cytochrome oxidation and shift in carotenoid absorption in purple bacteria. | | 1966 | 5971851 |
| studies of photosynthesis using a pulsed laser. i. temperature dependence of cytochrome oxidation rate in chromatium. evidence for tunneling. | the rate of oxidation of cytochrome following absorption of a short pulse of light from a ruby laser in the photosynthetic bacterium chromatium has been measured spectrophotometrically. the half-time is about 2 musec at room temperature increasing to 2.3 msec at about 100 degrees k and constant at the latter value to 35 degrees k or below. the temperature dependence above 120 degrees k corresponds to an activation energy of 3.3 kcal/mole; that below 100 degrees k to less than 80 cal/mol: essenti ... | 1966 | 5972381 |
| resonance raman evidence for intramolecular electron transport from flavin to heme in flavocytochrome c-552 and nature of chromophoric interactions. | | 1980 | 6257288 |
| high-potential iron-sulfur proteins and their possible site of electron transfer. | the electron-transfer mechanism of the fe4s4 high-potential iron-sulfur proteins (hipip's) was explored via a stopped-flow spectrophotometric kinetic study of the reduction of chromatium vinosum and rhodopseudomonas gelatinosa hipip's by both native and trinitrophenyllysine-13 horse cytochrome c. the influence of electrostatic effects was also effectively partitioned from the redox process per se. the corrected rates were 12.3 x 10(4) and 3.8 x 10(4) m-1 s-1 for native with c. vinosum and r. gel ... | 1981 | 6260144 |
| purification and some properties of the soluble hydrogenase from chromatium vinosum. | a routine procedure for the growth and harvesting of large (600 1) batches of chromatium vinosum and the isolation of hydrogenase (hydrogen: (acceptor) oxidoreductase, ec 1.12.-.-) are described. the enzyme is pure according to polyacrylamide gel electrophoresis, has a molecular weight of 61,000-63,000 and consists of a single polypeptide chain. the enzyme is stable in air but not active. activity is obtained only after complete removal of oxygen. epr spectroscopy at 9 ghz shows a signal indicat ... | 1981 | 6260199 |
| low-temperature magnetic circular dichroism spectra and magnetisation curves of 4fe clusters in iron-sulphur proteins from chromatium and clostridium pasteurianum. | the magnetic circular dichroism (mcd) spectra of the 4fe clusters in the iron-sulphur proteins high-potential iron protein from chromatium and the 8fe ferredoxin from clostridium pasteurianum have been measured over the wavelength range 300-800 nm at temperatures between approx. 1.5 and 50 k and at magnetic fields up to 5 tesla. in both cases the proteins have been studied in the oxidized and reduced states. the reduced state of high-potential iron protein gives a temperature-independent mcd spe ... | 1981 | 6260220 |
| the three-iron cluster in a ferredoxin from desulphovibrio gigas. a low-temperature magnetic circular dichroism study. | ferredoxin ii from desulphovibrio gigas is a tetrameric protein containing a novel iron-sulphur cluster consisting of three iron atoms. the low-temperature magnetic circular dichroism (mcd) spectra of the oxidized and dithionite-reduced forms of ferredoxin ii have been measured over the wavelength range approx. 300-800 nm. both oxidation levels of the cluster are shown to be paramagnetic, although only the oxidized form gives an epr signal. mcd magnetization curves have been constructed over the ... | 1981 | 6268181 |
| regulation of electron transfer by sidedness-dependent surface ph. dependence of the rate of cytochrome c-555 reduction on h+ concentration in the surface region on the periplasmic side of photosynthetic membranes in whole cells, spheroplasts and chromatophores of chromatium vinosum. | | 1981 | 6270069 |
| characterization of electron donation to cytochrome c-555 in chromatium vinosum from ferrocyanide, tetramethylphenylenediamine and reduced dimethylquinone. effects of redox potential, ph and salt concentration. | 1. the dependences of the reduction of ferricytochrome c-555 in the reaction center-cytochrome c complex on the redox potential and ph were investigated using n,n,n',n'-tetramethyl-p-phenylenediamine (tmpd), ferrocyanide, and reduced 2,5-dimethyl-p-quinone as electron donors. 2. in the reduction of cytochrome c-555 by tmpd, the unprotonated form was the exclusive electron donor to the cytochrome with a second-order rate constant of 1.0 x 10(5) m-1.s-1. 3. ferrocyanide reduced cytochrome c-555 sl ... | 1982 | 6284722 |
| intramolecular electron transfer in chlorobium thiosulfatophilum flavocytochrome c. | the electron-transfer reactions of photoproduced lumiflavin semiquinone and fully reduced lumiflavin with oxidized chlorobium thiosulfatophilum flavocytochrome c have been studied by using laser flash photolysis. the chlorobium flavocytochrome c contains one heme and one flavin per mr 50 000, and thus the possibility exists for intramolecular electron transfer. we find a complex kinetic pattern which is consistent with the transient formation of a spectrally perturbed protein-bound flavin semiqu ... | 1982 | 6291583 |
| effect of oxygen on acetylene reduction by photosynthetic bacteria. | the effect of dissolved oxygen concentration on nitrogenase activity was studied in three species of photosynthetic bacteria. the o2 concentration in the cell suspension was measured with an o2 electrode inserted into the reaction vessel. acetylene reduction by whole cells of rhodopseudomonas capsulata, rhodospirillum rubrum, and chromatium vinosum strain d was inhibited 50% by 0.73, 0.32, and 0.26 microm o2, respectively. the inhibition of the activity by o2 in r. capsulata usually was reversed ... | 1981 | 6790517 |
| fast stages of photoelectric processes in biological membranes. iii. bacterial photosynthetic redox system. | chromatophores of photosynthetic bacteria rhodospirillum rubrum, rhodopseudomonas sphaeroides and chromatium minutissimum were associated with a collodion film impregnated with a decane solution of asolectin. a very short light flash inducing a single turnover of the chromatophore photosynthetic redox system was found to induce the formation of an electrical potential difference amounting to 60 mv, directed across the film as measured with an orthodox electrometer technique. the main phase of th ... | 1981 | 6793358 |
| preliminary x-ray studies on chromatium vinosum flavocytochrome c552. | | 1982 | 6300410 |
| partial purification and characterization of two soluble c-type cytochromes from chromatium vinosum. | two c-type cytochromes from chromatium vinosum have been partially purified and characterized. cytochrome c550, which appears to function as an electron carrier in the cyclic electron transport chain of this photosynthetic purple sulfur bacterium, has a molecular weight of approximately 15,000 and an oxidation-reduction midpoint potential (em) of +240 mv at ph 7.4. it has (in the reduced form) an alpha band at 550 nm and a beta band at 520 nm. cytochrome c551 is characterized by absorbance maxim ... | 1983 | 6301383 |
| rna ligase in bacteria: formation of a 2',5' linkage by an e. coli extract. | ligase activity was detected in extracts of escherichia coli, clostridium tartarivorum, rhodospirillum salexigens, chromatium gracile, and chlorobium limicola. ligase was measured by joining of trna halves produced from yeast ivs-containing trna precursors by a yeast endonuclease. the structure of trnatyr halves joined by an e. coli extract was examined. the ligated junction is resistant to nuclease p1 and rnaase t2 but sensitive to venom phosphodiesterase and alkaline hydrolysis, consistent wit ... | 1983 | 6347395 |
| thioredoxin system of the photosynthetic anaerobe chromatium vinosum. | chromatium vinosum, an anaerobic photosynthetic purple sulfur bacterium, resembles aerobic bacterial cells in that it has an nadp-thioredoxin system composed of a single thioredoxin which is reduced by nadph via nadp-thioredoxin reductase. both protein components were purified to homogeneity, and some of their properties were determined. chromatium vinosum thioredoxin was slightly larger than other bacterial thioredoxins (13 versus 12 kilodaltons) but was similar in its specificity (ability to a ... | 1984 | 6373736 |
| comparison of the properties of two hydrogenases from the photosynthetic bacterium chromatium vinosum. | chromatium vinosum hydrogenases i and ii were purified to specific activities of 9.6 and 28.0 units/mg protein, respectively. they have the same isoelectric point (pi = 4.1), and their visible spectra are typical of iron-sulfur proteins. hydrogenase ii in general was more stable than hydrogenase i. both enzymes lost their activities slowly during storage in air, and this inactivation was more apparent in preparations of hydrogenase i. bovine serum albumin helped to stabilize hydrogenase i agains ... | 1984 | 6385861 |
| hybridization of cloned rhodopseudomonas capsulata photosynthesis genes with dna from other photosynthetic bacteria. | the homology of rhodopseudomonas capsulata dna segments carrying photosynthesis genes with sequences present in total dna from certain other photosynthetic and non-photosynthetic bacterial species was determined by hybridization. r. capsulata dna fragments that carry loci for production of peptide components of the photosynthetic reaction center and light-harvesting i antenna complex were found to hybridize to dna from some photosynthetic species. however, fragments that carry carotenoid or bact ... | 1983 | 6406432 |
| x-ray diffraction studies on chromatophore membrane from photosynthetic bacteria. ii. comparison of diffraction patterns of photosynthetic units from various purple bacteria. | comparative x-ray diffraction studies, in conjunction with infrared absorption spectroscopy, were performed on chromatophores isolated from various purple photosynthetic bacteria in order to achieve a better understanding of the molecular structure of the photosynthetic unit. purple non-sulfur bacteria used were rhodospirillum rubrum, rhodospirillum molischianum, rhodopseudomonas sphaeroides, and rhodopseudomonas palustris. chromatophores of chromatium vinosum, as a typical example of purple sul ... | 1984 | 6425275 |
| x-ray diffraction studies on chromatophore membrane from photosynthetic bacteria. iii. basic structure of the photosynthetic unit and its relation to other bacteriochlorophyll forms. | we have performed x-ray diffraction studies on photosynthetic units of rhodospirillum rubrum and solubilized *b800 + b890 complex from chromatophores of chromatium vinosum, to investigate the homology of their molecular structures. the native chromatophores of chromatium vinosum, which contain other bacteriochlorophyll forms, were examined by an x-ray diffraction technique, in order to assess the interactions between the complexes as well as the molecular structures of the bacteriochlorophyll fo ... | 1984 | 6442292 |
| characterization of the cell wall and cell wall proteins of chromatium vinosum. | highly purified cell walls of chromatium vinosum were isolated by differential centrifugation, with or without triton x-100 extraction. the isolated material had a protein composition similar to that of cell walls obtained by sucrose density gradient centrifugation. twenty-two proteins were reproducibly detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. a 42-kilodalton protein was shown to account for 65% of the total cell wall protein. the majority of cell wall proteins were ... | 1980 | 6988413 |
| [molecular organization of bacteriochlorophyll in the light-converging b850 complex of purple bacteria]. | the light-harvesting bacteriochlorophyll-protein complex b850 has been isolated from two species of purple bacteria, rhodopseudomonas palustris and chromatium minutissimum. absorption and fluorescence spectra at 20 degrees and--196 degrees c of this complex were registered. second derivatives of the absorption spectra, stepanov's relation and computer curve analyses in terms of asymmetrical gaussian components show that absorption spectra consist of five and fluorescence spectra--of three compon ... | 1984 | 6472267 |
| interaction of ribulose bisphosphate carboxylase/oxygenase with 2-carboxyhexitol 1,6-bisphosphates. | 2-c-carboxy-d-glucitol 1,6-bisphosphate (cgbp) and 2-c-carboxy-d-mannitol 1,6-bisphosphate (cmbp) have been synthesized, isolated, and the structures of these compounds and the derived lactones elucidated by nmr spectroscopy and periodate oxidation. both carboxyhexitol bisphosphates, which are homologs of the transition state analog 2-c-carboxy-d-arabinitol 1,5-bisphosphate, exhibit competitive inhibiton of ribulose bisphosphate carboxylase/oxygenase (ec 4.1.1.9) isolated from spinach (spinacia ... | 1983 | 6573158 |
| [effect of deuterization and possible water molecule participation in cytochrome c oxidation]. | | 1980 | 6249399 |
| chromatium vinosum cytochrome c-552. reduction by photoreduced flavins and intramolecular electron transfer. | cytochrome c-552 from chromatium vinosum is an unusual heme protein in that it contains two hemes and one flavin per molecule. to investigate whether intramolecular electron transfer occurs in this protein, we have studied its reduction by external photoreduced flavin by using pulsed-laser excitation. this approach allows us to measure reduction kinetics on the mirosecond time scale. both fully reduced lumiflavin and lumiflavin semiquinone radical reduce cytochrome c-552 with second-order rate c ... | 1980 | 6250567 |
| electron transfer reactions of high-potential iron-sulfur proteins and c-type cytochromes. | studies of electron transfer by biological oxidation-reduction proteins frequently focus on the interaction of a particular protein with nonphysiological oxidants and/or reductants. this approach, although valuable, is limited by the size and chemistry of the nonphysiological reactants. to further the understanding of biological electron transfer, we have investigated the interaction of two examples of high-potential iron-sulfur proteins (hipip's) with mitochondrial cytochrome c (horse heart) an ... | 1980 | 6252957 |
| characterization and stability of hydrogenase from chromatium. | the absorption spectrum of the hydrogenase from chromatium, which contains four iron atoms and four atoms of acid-labile sulfide, in 80% dimethylsulfoxide or hexamethylphosphoramide suggests the presence of a single [4fe-4s] cluster. the epr spectra of the oxidized enzyme in air, argon or carbon monoxide are the same with signals centered at g = 2.01. the enzyme reduced by hydrogen is epr silent. the epr spectrum is consistent with a [4fe-4s] cluster. chromatium hydrogenase and the hydrogenase f ... | 1980 | 6254569 |
| cytochromes and anaerobic sulfide oxidation in the purple sulfur bacterium chromatium warmingii. | two soluble acidic c-type cytochromes--c' and c-552--were isolated by ion exchange chromatography, gel filtration and ammonium sulfate fractionation. cytochrome c' is a high-spin cytochrome with maxima at 399 nm, 490 nm, and 634 nm in the oxidized form and at 550 nm, 425 nm and a characteristic shoulder at 434 nm in the reduced state. the best purity index obtained (a280/a399) was 0.35. cytochrome c' is autoxidizable, has a molecular weight of 12000 (estimated by sodium dodecylsulfate electropho ... | 1983 | 6670358 |
| molecular properties of high potential iron sulfur protein of chromatium warmingii. | high potential iron sulfur protein (hipip) of the purple sulfur bacterium chromatium warmingii was purified to homogeneity by ion exchange chromatography, gel filtration and ammonium sulfate fractionation. the acidic protein was isolated in the reduced form. the best purity index (a280/a388) obtained was 2.52, and 3.8 mumol of the protein was isolated out of 100 g wet cell material. the molecular weights estimated by sodium dodecylsulfate polyacrylamide gel electrophoresis and gel filtration thr ... | 1983 | 6670359 |
| inhibition of photosynthetic sulfide oxidation by organic cations. | photosynthetic sulfide oxidation by the purple sulfur bacterium chromatium vinosum is strongly inhibited by the organic cations benzyl viologen ( bv2 +) and tetraphenylphosphonium (tpp+) at micromolar concentrations. both are much more inhibitory at ph 8 than at ph 7. inhibition probably results from uptake of benzyl viologen and tetraphenylphosphonium in response to an electrical potential gradient across the plasma membrane which increases in magnitude with increasing ph. although both compoun ... | 1984 | 6743318 |
| resonance raman characterization of a novel, oxygen-binding heme protein from chromatium vinosum. | resonance raman spectroscopy was employed to characterize the local heme environment of a high-spin, ligand-binding heme protein from chromatium vinosum (chromatium high-spin hemoprotein). high-frequency spectra obtained with both b- and q-band excitation were found to resemble qualitatively those of deoxyhemoglobin (hba). differences between hba and chromatium high-spin hemoprotein spectra can be assigned to either the effects of a covalent linkage of the heme vinyls to the protein matrix or al ... | 1984 | 6743664 |
| energy transfer and bacteriochlorophyll fluorescence in purple bacteria at low temperature. | emission spectra of bacteriochlorophyll a fluorescence and absorption spectra of various purple bacteria were measured at temperatures between 295 and 4 k. for rhodospirillum rubrum the relative yield of photochemistry was measured in the same temperature region. in agreement with earlier results, sharpening and shifts of absorption bands were observed upon cooling to 77 k. below 77 k further sharpening occurred. in all species an absorption band was observed at 751-757 nm. the position of this ... | 1980 | 6772218 |
| [linear dichroism of pigments associated with spherical chromatophores. models of orientation in polyacrylamide gels]. | linear dichroism and orientation of pigments in chromatophores of photosynthetic bacteria chromatium minutissimum and rhodospirillum rubrum using a novel method of orientation in polyacrylamide gel was studied. a model is proposed for orientation of spherical membranes of chromatophores or other similar vesicules. the value of linear dichroism is derived for known deformation of the gel and a certain angle between the transition dipole and a unit vector perpendicular to the membrane plane. the a ... | 1980 | 6772937 |
| [kinetics of the generation of a photo-induced electric potential in chromatophores of photosynthetizing bacteria]. | flash-induced formation of an electric potential difference (delta psi) was monitored by a direct method in chromatophores associated with the collodion phospholipid membrane. in rhodospirillum rubrum and rhodopseudomonas sphaeriodes chromatophores, the kinetics of delta psi generation exhibit fast (tau less than or equal to 0.3 microseconds) and slow (tau congruent to 200 microseconds) phases, the latter observed in the presence of exogenous quinones. comparison of the kinetic and potentiometri ... | 1981 | 6789146 |
| isolation of a stable enzyme.14co2.mg2+.carboxyarabinitol bisphosphate complex with ribulosebisphosphate carboxylase from chromatium vinosum. | higher plant-type ribulosebisphoshate carboxylase from chromatium vinosum formed a stable, nonexchangeable complex with activator 14co2 in the presence of mg2+ and 2-carboxyarabinitol bisphosphate, an analog of the proposed transition-state intermediate. the response of the procaryotic enzyme to this analog was indistinguishable from that of the higher-plant carboxylase, which should permit comparative analysis of the activator site amino acid sequence in the two proteins. | 1982 | 6801011 |
| biosynthetic mechanism of ribulose-1,5-bisphosphate carboxylase in the purple photosynthetic bacterium, chromatium vinosum. | | 1982 | 6807199 |
| photosynthetic bacterial reaction centers: interactions among the bacteriochlorophylls and bacteriopheophytins. | | 1982 | 6808895 |
| proton efflux coupled to dark h2 oxidation in whole cells of a marine sulfur photosynthetic bacterium (chromatium sp. strain miami pbs1071). | whole cells of photoanaerobically grown chromatium sp. strain miami pbs1071, a marine sulfur purple bacterium, oxidized h2 in the dark through the oxyhydrogen reaction at rates of up to 59 nmol of h2 per mg (dry weight) per min. h2 oxidation was routinely measured in h2 pulse experiments with air-equilibrated cells. the reaction was accompanied by a reversible h+ efflux from the cells, suggesting an outward h+ translocation reaction coupled to h2 oxidation. the h+/e- ratio, calculated from simul ... | 1983 | 6833176 |
| comparative nuclear magnetic resonance studies of high potential iron-sulfur proteins from chromatium vinosum and rhodopseudomonas gelatinosa. additional hyperfine shifted resonances and ph-dependent structural perturbations. | proton nmr spectra and their dependence on ph are reported for the oxidized and reduced forms of the high potential iron-sulfur proteins from chromatium vinosum and rhodopseudomonas gelatinosa. spectra of the protein from both species are very similar in the regions occupied by the hyperfine shifted resonances of protons located near the [fe4s4(s-cys)4] cluster. the oxidized proteins exhibit three new resonances that had not been previously detected, one at very low field (about 100 ppm) and two ... | 1983 | 6863288 |
| biosynthetic mechanism of ribulose-1,5-bisphosphate carboxylase in the purple photosynthetic bacterium, chromatium vinosum. iii. absence of extrachromosomal dna. | inducible formation of ribulose-1,5-bisphosphate (rubp) carboxylase in the cells of chromatium vinosum under autotrophic conditions was not affected by six different inhibitors of dna synthesis. photosynthetic co2 fixation and rubp carboxylase activities were not influenced by seven reagents known to eliminate plasmids. plasmids were not detectable by agarose gel electrophoresis employing either the cleared lysate or alkaline sodium dodecyl sulfate method, nor were they detected by ethidium brom ... | 1983 | 6870250 |
| in vivo 31p and 13c nuclear magnetic resonance studies of acetate metabolism in chromatium vinosum. | 31p and 13c nuclear magnetic resonance (nmr) experiments were performed on suspensions of the phototrophic bacterium chromatium vinosum incubated anaerobically in the dark. 31p nmr spectra revealed that during prolonged dark incubation high atp levels are maintained. this phenomenon was independent of the presence of the energy reserves polyglucose and polyphosphate. 13c nmr experiments revealed that the amino acids glutamate, aspartate, and alanine are the major products of acetate incorporatio ... | 1983 | 6874640 |
| [localization of polypeptides from the pigment-protein complexes of chromatium minutissimum]. | | 1983 | 6884177 |
| properties of the hydrogenase of megasphaera elsdenii. | the catalytic activities of megasphaera elsdenii hydrogenase are stimulated by salts. the stimulation is due to the anion: the more chaotropic the anion, the greater the effect. dithionite-reduced and dye-oxidised preparations of hydrogenase are inactivated by reaction with oxygen. the inactivation of the reduced enzyme by excess oxygen follows pseudo-first-order kinetics; the reaction order for the oxidised enzyme has not been established. the rate of oxygen-inactivation is decreased by bovine ... | 1980 | 6995113 |
| isolation of two hydrogenase activities in chromatium. | kinetic, chromatographic and electrophoretic studies of chromatium hydrogenase show the existence in vitro of two different activities (i and ii). the two hydrogenases exhibit different kinetic parameters and properties. using reduced methyl viologen, km and [s]0.5 values of about 20 microm and 360 microm were calculated for the hydrogenases i and ii, respectively. hill plots revealed that hydrogenase i followed classical hyperbolic (michaelis-menten) kinetics. however, a hill coefficient (h = 0 ... | 1981 | 7011805 |
| composition and development of the bacterial photosynthetic apparatus. | | 1981 | 7032006 |
| influence of the redox potential on the activity of clostridium pasteurianum and chromatium hydrogenases. | | 1982 | 7046637 |
| atp-dependent k+ uptake by a photosynthetic purple sulfur bacterium. | | 1982 | 7073281 |
| terminal steps of bacteriochlorophyll a phytol formation in purple photosynthetic bacteria. | four chemically different bacteriochlorophylls (bchls) a esterified with geranylgeraniol, dihydrogeranylgeraniol, tetrahydrogeranylgeraniol, and phytol have been detected by high-pressure liquid chromatography in cell extracts from rhodopseudomonas sphaeroides and chromatium vinosum. bchl a containing phytol is the principal component, and the other three bchls a comprise about 4% of the total bchls a in stationary-phase cells of r. sphaeroides and c. vinosum. the high levels of the minor pigmen ... | 1984 | 6609153 |
| l-aspartate transport in the photosynthetic bacterium chromatium vinosum. | the photosynthetic purple sulfur bacterium chromatium vinosum appears to contain two active transport systems for l-aspartate. the higher affinity system (s0.5 = 60 microm) appears to be an electrogenic aspartate/h+ symport and the lower affinity system (s0.5 = 220 microm) appears to involve an aspartate/na+ symport. in addition to a possible role in providing the driving force for aspartate uptake, transmembrane na+ gradients may also have allosteric effects on aspartate transport in c. vinosum ... | 1983 | 6614931 |
| polar lipids in phototrophic bacteria of the rhodospirillaceae and chromatiaceae families. | the polar lipids of photosynthetic purple bacteria of the genera chromatium, thiocapsa, thiocystis, ectothiorhodospira, rhodopseudomonas, rhodospirillum, and rhodomicrobium were analyzed. characteristic compositions of the polar lipids were found for most of the rhodospirillaceae and chromatiaceae species. phosphatidylethanolamine, phosphatidylglycerol, and cardiolipin were the major phospholipids in most species. phosphatidylcholine was present as a major component in all species of the genus e ... | 1982 | 7076618 |
| [high potential iron-sulfur protein from thiocapsa roseopersicina]. | a high potential protein (hipip) containing a [4fe-4s] cluster was obtained in a homogeneous state from the purple sulfur bacterium t. roseopersicina strain bbs. the epr and absorption spectra are specific for this type of proteins. in the absorption spectrum the a283/a390 ratio equals to 2.25; mr = 10 000, pi is 4.08, e0' = +328 mv. the reduced form of hipip was found to be more stable upon storage; t 1/2 is 40 min at 80 degrees c. hipip can be reduced by ascorbate, cysteine, 2-mercaptoethanol ... | 1983 | 6615927 |
| sodium-dependent alpha-aminoisobutyrate transport by the photosynthetic purple sulfur bacterium chromatium vinosum. | | 1982 | 7103510 |
| identification of inner- and outer-sphere reaction pathways in the reduction of iron-sulphur proteins with a chromium (ii)-macrocycle complex. | | 1982 | 7103972 |
| formation of super-reduced chromatium high-potential iron--sulphur protein in aqueous solution by pulse radiolysis. | both the oxidized and reduced forms of hipip (high-potential iron--sulphur protein) are reduced (approx. 30% yields) by eaq.- in a single-stage process, rate constants 1.7 x 10(10) and 1.8 x 10(10) m-1 . s-1 respectively, at 25 degrees c, ph 7.0 (5 mm-phosphate). super-reduced hipip, which is formed in the latter case, has a spectrum which closely resembles that of reduced ferredoxin, i.e. fe4s4 (sr)4(3-) clusters. the spectrum is stable over 2 s periods investigated. super-reduced hipip is reox ... | 1980 | 7213348 |
| study of electrogenic electron transfer steps in chromatophore membrane of chromatium vinosum by the response of merocyanin dye. | 1. electrogenic steps in photosynthetic cyclic electron transport in chromatophore membrane of chromatium vinosum were studied by measuring absorption changes of added merocyanin dye and of intrinsic carotenoid. 2. the change in dye absorbance was linear with the membrane potential change induced either by light excitation or by application of diffusion potential by adding valinomycin in the presence of k+ concentration gradient. 3. it was estimated that chromatophore membrane became 40--60 mv a ... | 1980 | 7236632 |
| the role of histidine-42 in the oxidation-reduction mechanism of chromatium vinosum high potential iron-sulfur protein. | the second order rate constants for the oxidation of high potential iron-sulfur protein (hipip) of chromatium vinosum by ferricyanide were determined as a function of ionic strength and ph. from the ionic strength results, calculations were done to correct the rate constant at each ph for the electrostatic interactions between hipip and ferricyanide. the electrostatic corrections are necessary since the charge of the protein changes as a function of ph and can mask the ionization of mechanistica ... | 1980 | 7236640 |
| a possible new mechanism of temperature dependence of electron transfer in photosynthetic systems. | a new theory for the electron transfer by the non-adiabatic process is formulated taking into account the origin shift and the frequency change of the vibration. the resultant formulas are quite similar to those of jortner (jortner, j. (1976) j. chem. phys. 64, 4860-4867) except that the free energy gap delta g is used instead of the energy gap delta e. by applying this theory to the photosynthetic electron transfer, the role of the remarkable temperature dependence of the electron transfer from ... | 1981 | 7236675 |
| circular dichroism of bacteriochlorophyll a in light harvesting bacteriochlorophyll protein complexes from chromatium vinosum. | bacteriochlorophyll (bchl) protein complexes containing light harvesting (lh) bchls were isolated from chromatium vinosum, and their cd spectra were measured in the near-infrared region. these isolated bchl protein complexes retained the cd signals of lh bchls that were observed in situ in chromatophores. the cd spectrum of fraction a (containing b890) consisted of paired positive and negative bands (a double cd) having a zero-crossing at 800 nm and a single negative band at around 900 nm attrib ... | 1981 | 7287660 |
| energy-dependent sodium efflux and sodium-dependent alpha-aminoisobutyrate transport in purple photosynthetic bacteria. | | 1981 | 7305368 |
| siroheme as an active catalyst in sulfite reduction. | siroheme extracted by acetone/hcl treatment of sulfite reductase from yeast and purified by column chromatography catalyzed the reduction of sulfite to thiosulfate and sulfide when coupled with a hydrogen-hydrogenase-methyl viologen system. the activity increased with decrease in ph from 7 to 4, and an apparent km value of 50 mm for sulfite was obtained. in contrast to sirohydrochlorin plus fe2+, addition of inorganic iron or 2,2'-bipyridine prior to the reduction reaction had scarcely any effec ... | 1981 | 7338517 |
| thermal stabilities of membrane-bound, solubilized, and artificially immobilized hydrogenase from chromatium vinosum. | | 1980 | 7362243 |
| ionic strength, ph, and the electrostatic correction of redox protein reaction rates. | | 1980 | 7378073 |
| [effect of light intensity on the content of bacteriochlorophyll and on the growth of phototrophic marine sulfur bacteria]. | eight strains of chromatiaceae isolated from marine sediments are cultivated under light intensities of 50-5000 lx. a decreased in the light intensity brings about an increase in the specific bacteriochlorophyll content and also in the length of development. in certain strains, the increase in pigment contents partly compensates for the loss in light intensity, up to the maximum concentration of bacteriochlorophyll. this mechanism is only a physiological compatibility which ensures the survival ... | 1980 | 7378941 |
| the activation of ribulose-1,5-bisphosphate carboxylase-oxygenase from spinach by oxygen. | the effect of oxygen on ribulose-1,5-bisphosphate carboxylase-oxygenase from spinach was investigated. both activities were deactivated by removal of oxygen and reversibly reactivated by oxygenation of the enzyme solution. the change in enzyme activities was accompanied by conformational changes as studied by the use of intrinsic and extrinsic fluorescent probes. the analysis of cysteine sulfhydryl groups accessible to 5,5'-dithiobis-(2-nitrobenzoic acid) revealed that the number of these groups ... | 1980 | 7385943 |
| [ratio of metals during changes in the metabolism of photosynthetic bacteria]. | the content of polyvalent metals was determined in the photosynthetic bacteria rhodopseudomonas capsulata (athiorhodaceae) and chromatium vanda (thiorhodaceae) isolated from the sediments of lake vanda in the antarctic when the conditions of growth, photoheterophic and photoautotrophic, were changed. the ratio between fe and mn, cu, v, ni, co, cr was studied. the utilization of all polyvalent metals, mn and cr in particular, relatively increased in the conditions of autotrophic growth. changes i ... | 1980 | 7393002 |
| the photosynthetic electron transfer chain of chromatium vinosum chromatophores: flash-induced cytochrome b reduction. | reduction of a cytochrome b following excitation by a single, short, near-saturating light flash has been demonstrated in chromatium vinosum chromatophores. the extent of reduction is increased by addition of antimycin. the cytochrome has an alpha-band maximum at 562 nm in the presence of antimycin. the cytochrome b reduction is most readily observed in the presence of antimycin at high redox potential when cytochrome c-555 is oxidised before excitation. under these conditions the half-time for ... | 1980 | 7397126 |