| the internal hydrogen ion concentration of thiobacillus thio-oxidans and survival after irradiation. | | 1966 | 5941155 |
| [35s]thiosulphate oxidation by thiobacillus strain c. | 1. thiobacillus strain c oxidized [(35)s]thiosulphate completely to sulphate. 2. during thiosulphate oxidation [(35)s]sulphate was formed more rapidly from (s.(35)so(3))(2-) than from ((35)s.so(3))(2-). (35)s disappeared less rapidly from thiosulphate with ((35)s.so(3))(2-) as substrate than with (s.(35)so(3))(2-). 3. thiosulphate labelled in both atoms was produced during ((35)s.so(3))(2-) oxidation, but not during (s.(35)so(3))(2-) oxidation. 4. no (35)s was precipitated as elementary sulphur ... | 1966 | 5941348 |
| energy coupling during sulphur compound oxidation by thiobacillus sp. strain c. | | 1966 | 5954374 |
| occurrence of the adenosine monophosphate inhibition of carbon dioxide fixation in photosynthetic and chemosynthetic autotrophs. | | 1966 | 5954698 |
| growth of some chemoautotrophic bacteria at different oxygen tensions. | | 1966 | 5955989 |
| acid production by thiobacillus thiooxidans. | | 1966 | 5958116 |
| growth of thiobacillus thiooxidans on glucose. | | 1966 | 5961860 |
| the initial product and properties of the sulfur-oxidizing enzyme of thiobacilli. | | 1966 | 5968172 |
| competitive inhibition of phosphoribulokinase by amp. | | 1966 | 5970515 |
| studies on adenosine-5'-phosphosulphate reductase from thiobacillus denitrificans. | | 1966 | 5970862 |
| taxonomy of the acidophilic thiobacilli. | | 1966 | 5971385 |
| formation of polythionates and their interrelationships during oxidation of thiosulfate by thiobacillus ferrooxidans. | | 1966 | 5972638 |
| oxidation of inorganic sulfur compounds by washed cell suspensions of thiobacillus ferrooxidans. | | 1966 | 5972649 |
| [occurrence of bacteria in underground waters in relation to the prevailing redox condition]. | | 1966 | 6002795 |
| [study on bacterial regeneration of ferric sulphate and on steeping of copper from ores]. | | 1966 | 6002912 |
| [the role of thionic bacteria in oxidation of sulphide ores of the kafanskoe bed]. | | 1966 | 6003003 |
| keto acids as growth-limiting factors in autotrophic growth of thiobacillus thiooxidans. | when the strictly autotrophic bacterium thiobacillus thiooxidans was grown on sulfur, keto acids accumulated in the medium until they reached an inhibitory level at which growth ceased. much greater growth was possible if these substances were continually dialyzed out of the medium. | 1967 | 6020564 |
| yield coefficients of thiobacillus neapolitanus in continuous culture. | thiobacillus neapolitanus, when grown in continuous culture with thiosulfate limiting growth, possessed an apparent maximal molar growth yield of 8.0 g (dry weight) per mole of thiosulfate. the substrate requirement for energy of maintenance was the highest yet reported, amounting to 21.8 mmoles of thiosulfate per g per hr. the molar growth yield, corrected for this maintenance energy requirement, was 13.9 g (dry weight) per mole of thiosulfate. it was concluded that substrate-level phosphorylat ... | 1967 | 6025430 |
| taxonomy of anaerobic thiobacilli. | | 1967 | 6034038 |
| role of thiobacillus ferrooxidans in the oxidation of sulfide minerals. | | 1967 | 6034412 |
| phosphorylation coupled to the oxidation of sulfite and 2-mercaptoethanol in extracts of thiobacillus thioparus. | | 1967 | 6036893 |
| catabolite repression in the facultative chemoautotroph thiobacillus novellus. | several fermentable carbon sources were found to give rise to catabolite repression of all enzymes implicated in thiosulfate oxidation in the facultative chemoautotroph, thiobacillus novellus. glucose was found to elicit a strong repression. glycerol, lactate, lactose, ribose, and pyruvate caused marked repression. in all cases, the repression could be relieved only by returning the cells to a medium devoid of such fermentable substrates. on the other hand, carbon compounds (amino acids and orga ... | 1967 | 6057804 |
| [study of microbiological oxidative processes in the degtiar copper-pyrite deposits]. | | 1967 | 6063203 |
| marine thiobacilli. i. isolation and distribution. | | 1967 | 6064043 |
| marine thiobacilli. ii. culture and ultrastructure. | | 1967 | 6064044 |
| phospholipids of thiobacillus thiooxidans. | cells and spent growth media from sulfur- and thiosulfate-grown cultures of thiobacillus thiooxidans were analyzed. the phosphatides were examined by thinlayer chromatography, and the products of their hydrolysis by hydrochloric acid and methanolic potassium hydroxide were separated by paper chromatography. the phospholipids in both cells and spent growth media were identified as phosphatidyl ethanolamine, phosphatidyl-n-monomethylethanolamine, phosphatidyl glycerol, and diphosphatidyl glycerol. ... | 1967 | 6066049 |
| thiobacillus thiooxidans cell wall amino acids and monosaccharides. | | 1967 | 6074409 |
| sulfur bacteria. | | 1982 | 6127733 |
| microbiology of thiobacilli and other sulphur-oxidizing autotrophs, mixotrophs and heterotrophs. | recent studies on the ecophysiology of the obligate chemolithotroph thiobacillus neapolitanus have given better insight into its specialization for an autotrophic mode of life. this appears not only from its high constitutive levels of autotrophic enzymes, but also from its possession of carboxysomes, which seem to be specialized organelles for co2 fixation and concentrating reducing power. at the same time, these organisms are metabolically versatile with respect to nitrogen assimilation pathwa ... | 1982 | 6127737 |
| biochemistry of the chemolithotrophic oxidation of inorganic sulphur. | a historical review is presented of the elucidation of the mechanisms of oxidation of inorganic sulphur compounds and of electron transport in the thiobacilli. a unitary mechanism, consistent with current knowledge, is proposed. the significance of polythionates is discussed. the relations between oxidation mechanisms, substrate-level and electron transport-dependent phosphorylation, energy-dependent nad+ reduction and efficiency of growth are assessed in order to evaluate the efficiency of ener ... | 1982 | 6127738 |
| ecology of the bacteria of the sulphur cycle with special reference to anoxic-oxic interface environments. | h2s is produced as a main end-product of anaerobic mineralization in anoxic, sulphate-rich environments by a diverse population of sulphate-reducing bacteria. the sulphate reducers can carry out an almost complete oxidation of detrital organic matter to co2. the h2s consequently becomes an important electron carrier from the anoxic to the oxic world. thiobacilli and other colourless sulphur bacteria have the potential to oxidize the h2s at the oxic-anoxic interface in sediments or stratified wat ... | 1982 | 6127739 |
| assay of bacterial copper leaching from covellin at alkaline initial ph. | copper sulphide cus was leached by bacteria. beginning with an initial ph of 9.1, the bacteria acidified the environment and at the same time leached up to 5 g copper per liter. it is characteristic that the ph may sometimes stabilize at 4.0--5.0 and not fall below this value. | 1980 | 6155056 |
| obligate thymidine auxotroph of thiobacillus a2. | | 1981 | 6179400 |
| physicochemical characteristics of thiobacillus a2 dna. | | 1983 | 6194671 |
| a potentiometric and kinetic study on the respiratory chain of ferrous-iron-grown thiobacillus ferrooxidans. | the type and number of respiratory chain components present in membranes of thiobacillus ferrooxidans have been investigated. these redox components were resolved potentiometrically and kinetically. using optical techniques two cytochromes a1, multiple cytochromes c and two cytochromes b were detected. by using electron paramagnetic resonance, two copper-containing centres, high and low spin ferric haems and a ferredoxin centre were detected. based on the combination of a potentiometric resoluti ... | 1980 | 6245683 |
| cytochrome a-type terminal oxidase derived from thiobacillus novellus. molecular and enzymatic properties. | cytochrome a-type terminal oxidase was purified from thiobacillus novellus to an electrophoretically homogeneous state and some of its properties were studied. the enzyme shows absorption peaks at 428 and 602 nm in the oxidized form, and at 442 and 602 nm in the reduced form. the co compound of the reduced enzyme shows peaks at 431 and 599 nm. the enzyme has 1 mol of haem a and 1 g-atom of copper per 55600 g and is composed of two kinds of subunit, of 32000 and 23000 daltons, respectively. the e ... | 1980 | 6248108 |
| heme aa3-type cytochrome c oxidases from bacteria. | | 1980 | 6263325 |
| proton motive force and the physiological basis of delta ph maintenance in thiobacillus acidophilus. | at optimal growth ph (3.0) thiobacillus acidophilus maintained an internal ph of 5.6 (delta ph of 2.6 units) and a membrane potential (delta psi) of some +73 mv, corresponding to a proton motive force (delta p) of -83 mv. the internal ph remained poised at this value through external ph values of 1 to 5, so that the delta ph increased with decreasing external ph. the positive delta psi increased linearly with delta ph: above a delta ph of 0.6 units, some 60% of the increase in delta ph was compe ... | 1982 | 6279562 |
| oxidation-reduction potentials of respiratory chain components in thiobacillus a2. | (1) cells of thiobacillus a2 grown chemoautotrophically on thiosulfate or heterotrophically on succinate with oxygen contained b-, c-, o-, a- and a3-type cytochromes. the amount of cytochrome per mg of cell protein was much greater in thiosulfate-grown cells and differences in the relative concentrations of cytochromes were observed for the different growth conditions. (2) the half-reduction potentials at ph 7.0 (em,7.0) and spectral maxima of c-, b-, a- and a3-type cytochromes were similar in c ... | 1982 | 6284218 |
| mercuric reductase enzyme from a mercury-volatilizing strain of thiobacillus ferrooxidans. | cell-free mercury volatilization activity (mercuric reductase) was obtained from a mercury-volatilizing thiobacillus ferrooxidans strain, and the properties of intact-cell and cell-free activities were compared with those determined by plasmid r100 in escherichia coli. intact cells of t. ferrooxidans volatilized mercury at ph 2.5, whereas cells of e. coli did not. cell-free enzyme preparations from both bacteria functioned best at or above neutral ph and not at all at ph 2.5. the t. ferrooxidans ... | 1982 | 6286594 |
| mössbauer and epr studies on nitrite reductase from thiobacillus denitrificans. | | 1982 | 6286626 |
| effect of starvation on cytoplasmic ph, proton motive force, and viability of an acidophilic bacterium, thiobacillus acidophilus. | the question of whether thiobacillus acidophilus maintains its cytoplasmic ph at values close to neutrality by active or passive means was explored by subjecting the organism to long-term starvation (up to 22 days). starving cells maintained a delta ph of 2 to 3 u throughout starvation, although cellular poly-beta-hydroxybutyric acid and atp, the proton motive force, and culture viability were low or not detectable after 200 h. cells exposed to azide or azide plus n,n'-dicyclohexylcarbodiimide i ... | 1983 | 6294053 |
| thiobacillus ferrooxidans. the bioenergetics of an acidophilic chemolithotroph. | | 1982 | 6295474 |
| stable cosmid vectors that enable the introduction of cloned fragments into a wide range of gram-negative bacteria. | a cosmid cloning system has been developed which is useful for the construction of genomic libraries and the introduction of clones into a broad range of bacterial species. the cosmids pmmb33 and pmmb34 allow selective cloning into their unique bamhi site of 36-kb dna fragments generated by bamhi, sau3a and mboi partial digestion. this selective cloning is achieved by a strategy that avoids formation of polycosmids without a dephosphorylation step. it uses two unique recognition sites within the ... | 1983 | 6315541 |
| transfer of plasmid rp1 into chemolithotrophic thiobacillus neapolitanus. | rp1, a broad-host-range incompatibility group p1 plasmid specifying multiple drug resistances, has been transferred into the chemolithotrophic bacterium thiobacillus neapolitanus. the ability of t. neapolitanus to receive, express, and transmit rp1-encoded antibiotic resistances was examined. the data show that this obligate chemolithotroph can accept, replicate, and express heterologous plasmid dna from a heterotrophic bacterium. | 1983 | 6352686 |
| cloning of a thiobacillus ferrooxidans plasmid in escherichia coli. | three separate plasmids of 6, 7, 16, and greater than 23 kilobases were purified from a single clone of thiobacillus ferrooxidans atcc 33020 grown in the presence of uranium. the 6.7-kilobase plasmid (ptf1) was cloned separately into the hindiii or bamhi site of escherichia coli plasmid pbr322. restriction maps of the recombinant plasmids, termed ptf100 and ptf110, respectively, were constructed, creating potential cloning vehicles for exchanging genetic information between e. coli and t. ferroo ... | 1984 | 6361001 |
| energy conservation in acidophilic bacteria. | | 1983 | 6363899 |
| physiology of acidophilic and alkalophilic bacteria. | | 1983 | 6364726 |
| expression of a thiobacillus ferrooxidans origin of replication in escherichia coli. | a cryptic plasmid from an autotrophically grown arsenic-resistant strain of thiobacillus ferrooxidans was isolated and cloned into pbr325. the origin of replication of pbr325 was deleted, and the recombinant plasmid was shown to replicate in escherichia coli, using an origin of replication located on the thiobacillus plasmid. | 1984 | 6373729 |
| the acidophilic thiobacilli and other acidophilic bacteria that share their habitat. | | 1984 | 6388492 |
| r68.45 plasmid mediated conjugation in thiobacillus a2. | plasmid r68.45 classified into the incp-1 group which is characterized by a broad host range, was transferred from pseudomonas aeruginosa pa025 or escherichia coli into thiobacillus a2 by conjugation. the r plasmid was stably maintained in thiobacillus a2 but only neomycin/kanamycin resistance was fully expressed in the new host. conjugational transfer of r68.45 between different thiobacillus a2 strains was observed. r plasmid mediated transfer of chromosomal markers has been demonstrated. | 1984 | 6442386 |
| oxidation-reduction potentials and spectral properties of some cytochromes from thiobacillus versutus (a2). | cytochromes c-550 (acidic), c-550 (basic), c-551 and c-552.5 from thiobacillus versutus have been highly purified and characterized. their spectral properties at 77 k are described. oxidation-reduction titrations of cytochromes c-550 (acidic) and c-550 (basic) showed them to exhibit nernst values of n = 1, with single redox centres in the cytochromes, and to have midpoint redox potentials at ph 7.0 (em,7) of 290 and 260 mv, respectively. cytochrome c-551 contained two separately titratable redox ... | 1984 | 6498181 |
| proposed structure for the noncovalently associated heme prosthetic group of dissimilatory nitrite reductases. configuration of substituents of acrylochlorin. | the configuration of the substituents around the macrocycle core has been determined for acrylochlorin, the reduced porphyrin that is the iron-free form of heme d1. this has been accomplished by two independent techniques. nuclear overhauser enhancements on the proton resonances of acrylochlorin d3-methyl ester were measured to determine nearest-neighbor substituents and mesoprotons. a lanthanide shift reagent, tris-(1,1,1,2,2,3,3-heptafluoro-7,7-dimethyloctane-4,6-di onato) europium(iii), was f ... | 1984 | 6549007 |
| genetic transformation of obligately chemolithotrophic thiobacilli. | genetic transformation of thiobacaillus thioparus auxotrophs to prototrophy was obtained at frequencies of up to 10(-2) when proliferating cell populations were exposed to chromosomal dna from a nutritionally independent strain of the same bacterium. the rate at which transformation occurred depended on recipient growth rate and could be drastically reduced by depriving otherwise competent cells of either nitrogen or exogenous energy substrate. interspecies marker transfer was also shown among s ... | 1983 | 6571832 |
| [identification and distribution of sulfur in thiobacillus ferrooxidans cells]. | sulfur localization was studied in thiobacillus ferrooxidans cells grown in the medium 9k containing elemental sulfur. when sulfur is oxidized to sulfuric acid, it is located on the surface of the cell wall and within the cell wall at different levels of the periplasmic space, as well as in large spherical structures arranged at the poles or in the center of the cell. the authors discuss the mechanism of sulfur transport and distribution in t. ferrooxidans cells when sulfur is oxidized to sulfur ... | 1983 | 6621423 |
| sulfite oxidase activity in thiobacillus novellus. | thiobacillus novellus shows a maximum induction of sulfite oxidase activity and a maximum growth rate as a result of supplementing the autotrophic growth medium with 4.0 microm ammonium molybdate. cells grown in the presence of molybdate showed approximately 10-fold increases in the amount of enzyme-associated molybdenum and in the sulfite-to-cytochrome c and sulfite-to-ferricyanide reductase activities. the effect of exogenous molybdate was not discernible with cells grown in the absence of thi ... | 1983 | 6630156 |
| use of carboxylic acids by thiobacillus a2. | thiobacillus a2 can grow on acetate, glycollate, succinate and citrate as sole carbon and energy sources. results of growth and transport experiments indicated that separate transport systems existed for the four acids although acetate uptake by bacteria grown on glycollate was very rapid. citrate was a potentially toxic substrate in that low concentrations had to be supplied to adapt organisms to growth on citrate following autotrophic culture on thiosulphate. apparent ks values for transport b ... | 1983 | 6633275 |
| cytoplasmic ph homeostasis in an acidophilic bacterium, thiobacillus acidophilus. | the cytoplasmic buffering capacity of thiobacillus acidophilus (along with membrane properties) is responsible for the cytoplasmic ph homeostasis in metabolically compromised cells. when a large influx of h+ occurs, the cytoplasmic buffering capacity prevents drastic changes in ph; in addition, this influx, by increasing the positive membrane potential, eventually leads to a cessation of further h+ influx. | 1983 | 6643393 |
| an enzymatic lysis procedure for the assay of enzymes in thiobacillus a2. | a micromethod is described for the production of lysed preparations of thiobacillus a2 following treatment with lysozyme and edta. these may be used for the assay of intra-cellular enzymes including rhodanese, hexokinase, glucose 6-phosphate dehydrogenase and phosphoglucoseisomerase. the procedure is useful for assaying enzymes in samples too small to be treated by conventional mechanical methods, but gives comparable recoveries of enzyme activities. | 1983 | 6645982 |
| [nature of the sulfur-containing component and its function in thiobacillus ferrooxidans]. | as was shown using various reagents (ag+, cd2+) and solvents (ethanol, methanol), thiobacillus ferrooxidans cells accumulate colloidal sulfur when they grow in the medium 9k containing elemental sulfur. colloidal sulfur is accumulated in the periplasmic space, in large, bipolarly arranged spherical structures and in simple invaginates of the cytoplasmic membrane. t. ferrooxidans cells accumulate the sulfur at a highest rate during the stationary phase of growth and can use it as a source of ener ... | 1983 | 6645991 |
| ornithine-containing lipids in thiobacillus a2 and achromobacter sp. | 20 bacterial strains (corresponding to 16 species) were screened for ornithine lipids. only two species (thiobacillus a2 and achromobacter sp.) turned out to contain ornithine lipids (2.71 mmol/100 g and 0.38 mmol/100 g bacterial dry weight, respectively). in both ornithine lipids, a 3-hydroxy fatty acid was amide-linked to the alpha-amino group of ornithine, a normal fatty acid was ester-linked to the 3-hydroxy group of the former. the predominant fatty acids were 18:1(11) and 3-hydroxy-20:1(13 ... | 1984 | 6698001 |
| the role of thiobacillus albertis glycocalyx in the adhesion of cells to elemental sulfur. | thiobacillus albertis, a newly characterized acidophilic thiobacillus sp., was found not to be dependent on physiological conditions such as ph, cellular energy, or peripheral cell envelope sulfhydryl groups for attachment to elemental sulfur (s0). heat-killed cells or those pretreated with sulfhydryl reagents (iodoacetate or iodoacetamide) were able to adhere to s0 in comparable numbers as assayed by epifluorescence microscopy. in addition, iodoacetate and iodoacetamide were found to be bacteri ... | 1984 | 6713305 |
| evolution of bacterial denitrification and denitrifier diversity. | little is known about the role of nitrate in evolution of bacterial energy-generating mechanisms. denitrifying bacteria are commonly regarded to have evolved from nitrate-respiring bacteria. some researchers regard denitrification to be the precursor of aerobic respiration; others feel the opposite is true. currently recognized denitrifying bacteria such as hyphomicrobium, paracoccus, pseudomonas and thiobacillus form a very diverse group. however, inadequate testing procedures and uncertain tax ... | 1982 | 6762849 |
| intergeneric evolutionary homology revealed by the study of protocatechuate 3,4-dioxygenase from azotobacter vinelandii. | protocatechuate 3,4-dioxygenase (ec 1.13.1.3) was purified to homogeneity from extracts of azotobacter vinelandii. the molecular weight of the oligomeric protein was estimated to be 510 000 by gel filtration and 480 000 by ultracentrifugation. the oligomer appears to be formed by association of equal amounts of nonidentical subunits which were estimated by sodium dodecyl sulfate gel electrophoresis to have respective molecular weights of 23 300 and 25 250. ten gram-atoms of iron was associated w ... | 1980 | 6766312 |
| effects of amino acids on thiobacillus acidophilus. ii. threonine deaminase. | biosynthetic l-threonine deaminase was partially purified 73-fold with a 60% recovery from thiobacillus acidophilus by ammonium sulfate fractionation and by sepharose 6b-c1 chromatography. the optimal ph for enzyme activity was between 9.0 and 10.0 and no optimal ph shift was observed in the presence of l-isoleucine, an inhibitor. the enzyme was effectively inhibited by l-isoleucine and showed homotropic interaction only in the presence of l-isoleucine. kinetic studies indicate that there are at ... | 1980 | 6773653 |
| [sulfur and iron cycling bacteria in low-sulfate meromictic lake kuznechikha]. | the hydrochemical characteristics, the composition of species and the localization of bacterial species involved in oxidation of sulfide and ferrous salts were studied in the meromictic lake kuznechikha with a low sulfate content in summer and in winter. the rate of bacterial sulfate reduction and the rates of bacterial and algal photosynthesis were determined using a radioisotope technique. the meromictic nature of the lake is due to the accumulation of ferrous salts (up to 212 mg/l fe2+) in th ... | 1980 | 6777648 |
| cell yield and bioenergetics of thiomicrospira denitrificans compared with thiobacillus denitrificans. | from cell yields of thiomicrospira denitrificans grown inthe chemostat at different growth rates under anaerobic conditions a value of 1.4 mm s2o3 = per g dry wt and per h could be calculated for maintenance energy requirements, and of 5.65 dry wt per mole s2o3 = for the true growth yield. cell yields of thiomicrospira denitrificans appeared to be almost half of those of thiobacillus denitrificans. though in thiobacillus denitrificans at d = 0.03 h(-1) under anaerobic conditions a value was foun ... | 1981 | 6791590 |
| carbon dioxide assimilation by thiobacillus novellus under nutrient-limited mixotrophic conditions. | the contribution of co2 to cell material synthesis in thiobacillus novellus under nutrient-limited conditions was estimated by comparing 14co2 uptake rates of steady-state autotrophic cultures with that of heterotrophic and mixotrophic cultures at a given dilution rate. under heterotrophic conditions, some 13% of the cell carbon was derived from co2; this is similar to the usual anaplerotic co2 fixation in batch cultures of heterotrophic bacteria. under mixotrophic conditions, the contribution o ... | 1982 | 6801026 |
| glucose transport system in a facultative iron-oxidizing bacterium, thiobacillus ferrooxidans. | properties of a heat-labile glucose transport system in thiobacillus ferrooxidans strain ap-44 were investigated with iron-grown cells. [14c]glucose was incorporated into cell fractions, and the cells metabolized [14c]glucose to 14co2. amytal, rotenone, cyanide, azide, 2,4-dinitrophenol, and dicyclohexylcarbodiimide strongly inhibited [14c]glucose uptake activity, suggesting the presence of an energy-dependent glucose transport system in t. ferrooxidans. heavy metals, such as mercury, silver, ur ... | 1982 | 6804437 |
| purification of thiobacillus novellus sulfite oxidase. evidence for the presence of heme and molybdenum. | sulfite oxidase from thiobacillus novellus has been purified 206-fold. the enzyme reduced both ferricyanide and cytochrome c. the ferricyanide activity was 3-5% of the cytochrome c activity. during purification, the absorbance ratio of a413 nm/a280 nm showed a continual increase, suggesting the presence of heme in the t. novellus sulfite oxidase molecule. the absorption spectrum of the enzyme is very similar to that of rat liver sulfite oxidase which contains cytochrome b5 type heme. gel electro ... | 1983 | 6853504 |
| sequences of the 5s rrnas of the thermo-acidophilic archaebacterium sulfolobus solfataricus (caldariella acidophila) and the thermophilic eubacteria bacillus acidocaldarius and thermus aquaticus. | we have determined the nucleotide sequences of the 5 s rrnas of three thermophilic bacteria: the archaebacterium sulfolobus solfataricus, also named caldariella acidophila, and the eubacteria bacillus acidocaldarius and thermus aquaticus. a 5 s rna sequence for the latter species had already been published, but it looked suspect on the basis of its alignment with other 5 s rna sequences and its base-pairing pattern. the corrected sequence aligns much better and fits in the universal five helix s ... | 1983 | 6878035 |
| [role of microorganisms in the destruction of spodumene]. | a broad spectrum of microorganisms has been shown to be involved in the destruction of spodumene, a typical mineral of lithium pegmatites. the following microorganisms are most active: the microscopic fungi penicillium notatum and aspergillus niger, the thiobacilli thiobacillus thiooxidans, and the so-called "silicate" slime forming bacterium bacillus micilaginosus n. sp. siliceus. spodumene destruction is accompanied with lithium, aluminium and silicon being transferred into solution. the activ ... | 1980 | 6995818 |
| ore leaching by bacteria. | | 1980 | 7002025 |
| localization of quantitation of the ornithine lipid of thiobacillus thiooxidans. | the ornithine lipid of thiobacillus thiooxidans was found to be 1.9% of the total polar lipids. approximately 80% of this lipid was found to be localized in the outer membrane. | 1982 | 7035440 |
| [effect of fe3+ ions on thiobacillus ferrooxidans oxidation of ferrous oxide at various temperatures]. | the inhibition of the rate of ferrous iron oxidation by thiobacillus ferrooxidans with ferric ions was shown to depend on their concentration, the temperature of the medium, and the phase of the cultural growth. ferrous iron oxidation was inhibited with ferric ions both at low (below 1.0 g/l) and high (ca. 10 g/l) concentrations, the process being temperature dependent: the rate of inhibition decreased with a fall of the temperature. the bacterium was most sensitive to unfavourable fe3+ concentr ... | 1982 | 7070305 |
| reactivity versus flexibility in thiobacilli. | the results of ecophysiological studies on obligately and facultatively chemolithotrophic thiobacilli performed over the past years clearly show that the two types of organisms occupy different ecological niches. chemostat experiments with cultures of the obligate chemolithotroph thiobacillus neapolitanus and the facultative chemolithotroph thiobacillus a2 have been carried out to explain the competitiveness of t. neapolitanus under conditions of strongly fluctuating substrate supply. thiobacill ... | 1982 | 7092201 |
| bacterial ribosephosphate isomerase. | | 1982 | 7144591 |
| [role of phospholipids in the fractionation of stable sulfur isotopes during oxidation by thiobacillus ferrooxidans]. | the action of thiobacillus ferrooxidans on elementary suhur was shown to be a step-wise process connected with the formation of its colloidal forms having a different isotope composition. certain sulfur forms are in complex with phospholipids. both exogenous and endogenous phospholipids are involved in the dissolving of sulfur and in the fractionation of its isotopes. exogenous phospholipids are more active. the authors discuss the role of phospholipids in the solution of sulfur and in its trans ... | 1982 | 7144609 |
| microbial transformations of inorganic sulphur compounds in soil under conditions of heterocontinuous cultivation. | development and activity of the association of the sulphur cycle bacteria, represented by thiobacillus thioparus and desulfovibrio sp., were followed in chernozem soil continuously supplemented with sodium thiosulphate. the technique of heterocontinuous cultivation made it possible (i) to determine changes in the individual components of microflora involved in successive metabolic steps, their time and space sequence, (ii) to follow changes in the transformations of substrate and formation of me ... | 1982 | 7173746 |
| thiobacilli and sulphate production from inorganic sulphur compounds in upper horizons of spruce forest soils. | the species representation of thiobacilli was investigated in horizons f, h and a of spruce forest at ten localities. concentrations of thiobacilli in the selected localities and ability of the soils to oxidize sulphur compounds to sulphate were determined. horizons f exhibited a high oxidative activity, a lower activity was found in horizon h and the lowest one was detected in horizon a. the activities showed spring and autumn maxima. horizons f, h and a contained 10(4)--10(5), 10(2)--10(3) and ... | 1981 | 7203285 |
| [fractionation of stable isotopes of sulfur during its oxidation by thiobacillus ferrooxidans]. | the fractionation of sulfur isotopes was studied in the process of sulfur oxidation by thiobacillus ferrooxidans. the isotope effect was observed at all stages of the bacterial growth during sulfur oxidation; as a result, residual sulfur became heavier whereas the fraction of colloidal sulfur and the fraction of sulfates became lighter. the isotope differences between the residual sulfur and the sulfates were greatest at the end of the lag phase (delta 34s = 0.38%) and the beginning of the stati ... | 1980 | 7207257 |
| physiological and ultrastructural characterization of a new acidophilic thiobacillus species (t. kabobis). | a new autotrophic acidophilic thiobacillus sp. was isolated from acidic soil adjacent to a natural gas processing plant's sulfur stockpile. this isolate metabolized s2o3(2-) to s4o6(2-) during growth and could not reoxidize this product; instead, the remaining s2o3(2-) substrate was oxidized to so4(2-) in the stationary phase which represented a significant metabolic change as compared with other acidophilic thiobacilli. in contrast the isolate oxidized s0 to h2so4 during the log-phase growth wi ... | 1981 | 7214235 |
| [oxidation of sulfide minerals by thiobacillus ferrooxidans]. | samples of natural pyrites and sphalerites were subjected to the action of the mineral medium 9k with 1 g of fe3+ per litre in the presence and in the absence of thiobacillus ferrooxidans, and incubated at 28 degrees c under the stationary conditions for 30 days. the chemical composition of the solutions was studied after leaching as well as changes of the surfaces of monoliths. the deepest etching of surfaces with the formation of crusts and films of jarosite, limonite and goslarite occurs upon ... | 1981 | 7219212 |
| [rate of iron (fe 2+) bacterial oxidation at different temperatures and concentrations of thiobacillus ferrooxidans cells]. | the rate of iron oxidation by bacteria thiobacillus ferrooxidans was measured at different temperatures (5 and 23 degrees c) and concentrations of bacterial cells (10(8)/ml and less). at a cell concentration of 10(7)--10(8)/ml the rate of bacterial oxidation remained at a relatively high level at a lowered temperature. at a low cell concentration (10(6)/ml and less) the rate of bacterial oxidation was reduced at a temperature of 5--6 degrees c. | 1980 | 7220514 |
| effect of industrial emissions with high sulphur dioxide content on thiobacilli and oxidative activity of spruce forest soils towards inorganic sulphur compounds. | effect of industrial emissions with high sulphur dioxide content on the upper horizons of spruce forest soils in nw bohemia was investigated. the content of sulphates, oxidative activity towards sulphide, elemental sulphur, thiosulphate and sulphite, concentration and species representation of thiobacilli in horizons f, h and a in regions highly affected by emissions (two localities) and in regions relatively less influenced (three localities) were followed. in the affected areas the sulphur con ... | 1981 | 7262715 |
| geomicrobiological leaching of tin minerals by thiobacillus ferro-oxidans and organic agents. | laboratory investigations confirm that it is possible to leach tin from synthetic minerals like stannite, kesterite, stannoidite, herzenbergite, ottemannite and berndtite, and from natural tin minerals which include stannite, cassiterite and varlamoffite, in the presence of thiobacillus ferrooxidans, and with organic agents of biological origin, especially oxalic acid and oxalic-citric acids mixture. over a leaching period of 35 days with 0.5% pulp density, initial ph of 2.5, using minus 0.16 mm ... | 1981 | 7269646 |
| plasmid dna in acidophilic, chemolithotrophic thiobacilli. | plasmid patterns were determined in 15 strains of iron-oxidizing thiobacillus ferrooxidans. in four of these strains plasmid dna was not detected. in the other strains the molecular weights of plasmids ranged from 5 x 10(6) to 50 x 10(6) and each strain had a different plasmid composition. the change of growth substrate from ferrous iron to tetrathionate did not affect the plasmid pattern in t. ferrooxidans nor did it in t. acidophilus, which was adapted from glucose to grow on tetrathionate. | 1981 | 7296416 |
| active transport of amino acids in thiobacillus thioparus is a low-affinity process. | a method for the isolation of amino acid auxotrophs of thiobacillus thioparus is described. characterization of a leucine auxotroph indicated that leucine biosynthesis in t. thioparus was not different from that of heterotrophic bacteria. t. thioparus cells accumulated amino acids via an active mechanism. kt values of amino acid transport were between 15 and 330 microm, and vmax values were 200 to 350 pmol min-1 mg of protein-1. amino acid transport was carried out by a limited number of systems ... | 1981 | 7309681 |
| regulation of amino acid transport in thiobacillus thioparus. | amino acid transport in amino acid auxotrophs of thiobacillus thioparus was enhanced during growth on rate-limiting amino acid concentration. a pleiotropic mutation enhanced general amino acid transport as manifested by higher values of vmax of amino acid transport. affinity constants remained unaltered. mutants with enhanced transport properties did not show changes in oxidation of thiosulfate, did not oxidize various organic compounds, and did not increase the heterotrophic potential of t. thi ... | 1981 | 7309682 |
| presence of two subunit types in ribulose 1,5-bisphosphate carboxylase from thiobacillus intermedius. | ribulose bisphosphate carboxylase (ec 4.1.1.39) has been purified to homogeneity from glutamate-co2-thiosulfate-grown thiobacillus intermedius by pelleting the protein from the 93,000 x g supernatant fluid followed by ammonium sulfate fractionation and sedimentation into a discontinuous sucrose density gradient. the molecular weight of the native protein approximated that of the higher plant enzyme (550,000) based on its relative electrophoretic mobility in polyacrylamide disc gels compared with ... | 1980 | 7364715 |
| adenosine 5'-triphosphate formation in thiobacillus ferrooxidans vesicles by h+ ion gradients comparable to those of environmental conditions. | vesicles prepared from iron-grown thiobacillus ferrooxidans, and subsequently loaded with adenosine 5'-diphosphate and inorganic phosphate, produced adenosine 5'-triphosphate when subjected to h+ gradients comparable to those in the cells' normal environment (i.e., an internal ph in the range of 6.0 to 8.0 with an optimum of 7.0 to 7.8 and an external ph in the range of 2.1 to 4.1 with an optimum of 2.8). nigericin, dicyclohexylcarbodiimide, and pentachlorophenol decreased adenosine 5'-triphosph ... | 1980 | 7372573 |
| growth of thiobacillus novellus on mixed substrates (mixotrophic growth). | in a mixotrophic environment, thiobacillus novellus concurrently utilized glucose and thiosulfate but showed no stimulation of growth rate or yield. in most mixotrophic environments examined, the growth rate was lower than the heterotrophic growth rate, the extent of the decrease depending on the concentration and relative proportion of thiosulfate and glucose in the medium. both thiosulfate and glucose were degraded to their most oxidized products in mixotrophic medium, yet the biomass producti ... | 1980 | 7380802 |
| regulation of glucose transport and metabolism in thiobacillus novellus. | to investigate the physiological basis of decreased rate of glucose utilization by thiobacillus novellus in a mixotrophic environment (r. c. perez and a. matin, j. bacteriol. 142:633-638, 1980), its glucose transport system was characterized and the modulation of this system as well as enzymes of glucose metabolism by the growth environment was examined. uptake of 2-deoxy-d-glucose by cell suspensions was almost abolished by respiratory chain inhibitors, and the sugar accumulated unchanged insid ... | 1980 | 7380803 |
| growth and physiology of thiobacillus novellus under nutrient-limited mixotrophic conditions. | thiobacillus novellus was cultivated in a chemostate under the individual limitations of thiosulfate, glucose, and thiosulfate plus glucose. at dilution rate (d) of 0.05 h-1 or lower, the steady-state biomass concentration in mixotrophic medium was additive of the heterotrophic and autotrophic biomass at corresponding d values. the ambient concentrations of thiosulfate, glucose, or both in the various cultures were low and were very similar in mixotrophic, heterotrophic, and autotrophic environm ... | 1980 | 7380804 |
| [thiobacillus thiocyanoxidans growth patterns in relation to the purification of industrial sewage]. | | 1980 | 7382861 |
| heterotrophic bacteria from cultures of autotrophic thiobacillus ferrooxidans: relationships as studied by means of deoxyribonucleic acid homology. | from several presumably pure cultures of thiobacillus ferrooxidans, we isolated a pair of stable phenotypes. one was a strict autotroph utilizing sulfur or ferrous iron as the energy source and unable to utilize glucose; the other phenotype was an acidophilic obligate heterotroph capable of utilizing glucose but not sulfur or ferrous iron. the acidophilic obligate heterotroph not only was encountered in cultures of t. ferrooxidans, but also was isolated with glucose-mineral salts medium, ph 2.0, ... | 1980 | 7400100 |
| [composition of a nutrient medium for the continuous cultivation of thiobacillus ferrooxidans]. | thiobacillus ferrooxidans was found to require the following biogenous elements for constructing its biomass: phosphorus, 14 +/- 2; magnesium, 2 +/- 0.5; potassium, 5 +/- 1; nitrogen, 100 +/- 10 (milligrams per gram of dry biomass). it was shown that the concentrations of biogenous elements in the growth medium, which did not limit the biosynthesis, did not exceed 5 divided by 10 mg per litre for all of the studied elements. the medium 9k that is commonly used for growing thiobacillus ferrooxida ... | 1980 | 7402126 |
| inhibition of ribulose-1,5-bisphosphate carboxylase-oxygenase activities by hydroxylamine. | hydroxylamine directly and reversibly inhibits both activities of homogeneous ribulose-1,5-bisphosphate carboxylase-oxygenase (3-phospho-d-glycerate carboxy-lyase (dimerizing), ec 4.1.1.39) isolated from diverse sources. nh2oh is an uncompetitive inhibitor of carboxylase activity with respect to ribulose-bisphosph ate. this reagent also reacts non-enzymically with ribulosebisphosphate to deplete this substrate. contrary to previous reports, these results indicate that hydroxylamine directly and ... | 1980 | 7407202 |
| substrate level versus oxidative phosphorylation in the generation of atp in thiobacillus denitrificans. | particulate fractions of thiobacillus denitrificans catalyse that the phosphorylation of adp to atp during the oxidation of various inorganic sulphur compounds or nadh via an electron transport chain. on the other hand, a "soluble" cell-free fraction synthesized atp from aps and inorganic phosphate. the production of atp was verified either by the firefly luciferin-luciferase enzyme system or by the incorporation of 32pi into atp. during the oxidation of sulphide, sulphite and nadh the productio ... | 1980 | 7458535 |
| thiobacillus acidophilus: a study of its presence in thiobacillus ferrooxidans cultures. | a study has been undertaken to account for the presence of thiobacillus acidophilus in iron-grown cultures of thiobacillus ferrooxidans. attempts to adapt t. acidophilus to ferrous iron were not successful but the facultative autotroph grew to a limited extent in the spent medium of t. ferrooxidans and was able to grow oligotrophically. possible oligotrophic substrates were methanol, ethanol, and sulphide. thiobacillus ferrooxidans may benefit from the presence of t. acidophilus because in mixed ... | 1980 | 7459720 |