| modifications of the permeability of the blood-brain barrier and local cerebral metabolism in pentobarbital- and ketamine-anaesthetized rats. | the state of deep surgical anaesthesia, induced by intraperitoneal injection of pentobarbital sodium (54 mg/kg) or ketamine hydrochloride (150 mg/kg) in the rat, was accompanied by a significant reduction in the permeability of the blood-brain barrier evaluated by calculating a unidirectional blood-to-brain constant (ki) for the circulating tracer [14c]alpha-aminoisobutyric acid. pentobarbital-induced anaesthesia was also characterized by a widespread and marked depression of local cerebral gluc ... | 1989 | 2812289 |
| lack of correlation of maternal human immunodeficiency virus infection with neonatal malformations. | a malformation syndrome has been proposed in infants with acquired immunodeficiency syndrome or acquired immunodeficiency syndrome-related complex secondary to congenital infection with human immunodeficiency virus (hiv) in the united states and europe. to determine whether embryopathy is detectable in hiv-exposed african infants, 85 infants of hiv-seropositive mothers and 98 infants of hiv-seronegative mothers in nairobi, kenya, were examined for minor and major anomalous features shortly after ... | 1989 | 2812915 |
| disease response to tobacco mosaic virus in transgenic tobacco plants that constitutively express the pathogenesis-related pr1b gene. | correlation of the temporal and spacial pattern of induction of the pathogenesis-related (pr) genes pr1a, pr1b, and pr1c with viral infections in certain tobacco cultivars has implicated pr proteins in viral disease resistance. to test whether the pr1 proteins of tobacco are involved in viral resistance, transgenic nicotiana tabacum plants were constructed which constitutively express the pr1b gene. this protein was secreted from cells of transgenic plants and accumulated in the extracellular sp ... | 1989 | 2815592 |
| disease response to tobacco mosaic virus in transgenic tobacco plants that constitutively express the pathogenesis-related pr1b gene. | correlation of the temporal and spacial pattern of induction of the pathogenesis-related (pr) genes pr1a, pr1b, and pr1c with viral infections in certain tobacco cultivars has implicated pr proteins in viral disease resistance. to test whether the pr1 proteins of tobacco are involved in viral resistance, transgenic nicotiana tabacum plants were constructed which constitutively express the pr1b gene. this protein was secreted from cells of transgenic plants and accumulated in the extracellular sp ... | 1989 | 2815592 |
| smoking and other tobacco use. | | 1989 | 2815598 |
| smoking and other tobacco use. | | 1989 | 2815598 |
| use of nude (athymic) mice for the study of hypertrophic scars and keloids: vascular continuity between mouse and implants. | hypertrophic scars and keloids appear to be unique to humans since animals are not known to form these lesions. therefore, in an effort to develop an experimental model for their study, implants of these human lesions were made in nude (athymic) mice (nu/nu) in suprascapular subcutaneous pockets. the implants were recovered from 2 to 246 days. by histological and fine structural parameters all implants remained viable and their morphological character was maintained. selected mice were injected ... | 1989 | 2817436 |
| [selected aspects of acid and temperature resistance of the bovine leukosis virus]. | | 1987 | 2820338 |
| the molybdenum-pterin binding protein is encoded by a multigene family in clostridium pasteurianum. | there are three variants of the molybdenum-pterin binding protein (mop) encoded by three distinct genes in clostridium pasteurianum. nucleotide sequence analysis shows that the three mop genes have greater than 90% homology at the nucleotide level. upstream from the coding region of each mop gene are potential promoter consensus sequences. analysis of mop purified from cells grown under nitrogen-fixing conditions indicates all three genes are expressed. sequence analysis of the three mop genes a ... | 1987 | 2820842 |
| identification of an additional sendai virus non-structural protein encoded by the p/c mrna. | peptide antiserum and monoclonal antibodies have detected a previously unrecognized small non-structural protein in sendai virus-infected cells. this protein, designated x, appears to represent the c-terminal 95 amino acids of the p protein reading frame. the x protein appears to be almost as abundant as the p protein on a molar basis in vivo. no evidence of a precursor-product relationship between the x and p proteins could be found. | 1987 | 2821189 |
| virological implications of the use of primates in xenotransplantation. | | 1987 | 2823433 |
| virological implications of the use of primates in xenotransplantation. | | 1987 | 2823433 |
| effects of tilomisole, indomethacin and levamisole on regulation of epstein barr virus-induced b cell proliferation by peripheral blood mononuclear cells from normal individuals and patients with rheumatoid arthritis. | when activated in autologous mixed leukocyte reactions (auto-mlr) in vitro, t cells from normal individuals produce a suppressor factor(s) which inhibits the epstein-barr virus (ebv)-induced proliferation of normal b cells. in contrast, t cells from patients with rheumatoid arthritis (ra) are deficient in their ability to generate this suppressor factor in auto-mlr. addition of tilomisole (wy-18,251; 33(p-chlorophenyl)thiazolo[3,2-a]benzimidazole-2-acetic acid) to the auto-mlr (0.1-100 microgram ... | 1987 | 2825478 |
| effects of tilomisole, indomethacin and levamisole on regulation of epstein barr virus-induced b cell proliferation by peripheral blood mononuclear cells from normal individuals and patients with rheumatoid arthritis. | when activated in autologous mixed leukocyte reactions (auto-mlr) in vitro, t cells from normal individuals produce a suppressor factor(s) which inhibits the epstein-barr virus (ebv)-induced proliferation of normal b cells. in contrast, t cells from patients with rheumatoid arthritis (ra) are deficient in their ability to generate this suppressor factor in auto-mlr. addition of tilomisole (wy-18,251; 33(p-chlorophenyl)thiazolo[3,2-a]benzimidazole-2-acetic acid) to the auto-mlr (0.1-100 microgram ... | 1987 | 2825478 |
| opioid receptors inhibit the adenylate cyclase in guinea pig cochleas. | the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ... | 1987 | 2825909 |
| opioid receptors inhibit the adenylate cyclase in guinea pig cochleas. | the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ... | 1987 | 2825909 |
| modulating action of cyclic amp on the expression of two sos genes in escherichia coli k-12. | ultraviolet irradiation and cyclic amp treatment produce a synergistic effect on the induction of the cle1 gene (coding for bacteriocin cole1) in wild-type strains of escherichia coli. on the other hand, cyclic amp does not affect the uv-mediated induction of the reca, sfia, and umudc genes. growth in the presence of glucose or glycerol does not affect the factor of amplification of the expression of the cle1 gene in uv-irradiated cells of the wild-type strain. although, in cultures not treated ... | 1987 | 2825953 |
| modulating action of cyclic amp on the expression of two sos genes in escherichia coli k-12. | ultraviolet irradiation and cyclic amp treatment produce a synergistic effect on the induction of the cle1 gene (coding for bacteriocin cole1) in wild-type strains of escherichia coli. on the other hand, cyclic amp does not affect the uv-mediated induction of the reca, sfia, and umudc genes. growth in the presence of glucose or glycerol does not affect the factor of amplification of the expression of the cle1 gene in uv-irradiated cells of the wild-type strain. although, in cultures not treated ... | 1987 | 2825953 |
| subcellular localization of enzymes in streptomyces aureofaciens and its alteration by benzyl thiocyanate. i. phosphatases and atp-glucokinase. | mycelia of a low- and a high-production strain of streptomyces aureofaciens were converted into protoplasts and divided into five subcellular fractions in order to localize exopolyphosphatases (ec 3.6.1.11), triphosphatase (ec 3.6.1.25), inorganic diphosphatase (ec 3.6.1.1), apyrase (ec 3.6.1.5) and glucokinase (ec 2.7.1.2). the highest specific activity of enzymes hydrolyzing polyphosphates was found in cytoplasmic vesicles and membranes. triphosphatase was detected in the periplasmic fraction. ... | 1987 | 2826319 |
| the sequence of chick alpha-actinin reveals homologies to spectrin and calmodulin. | we have sequenced a cdna, isolated from a chick embryo fibroblast lambda gt11 library, that encodes all 887 amino acids of alpha-actinin. sequence from 10 different peptides from chick smooth muscle alpha-actinin was found to match that derived from the cdna. the deduced protein sequence can be divided into three distinct domains: (a) the n-terminal 240 amino acid contains a highly conserved region (compared with dictyostelium alpha-actinin) which probably represents the actin-binding domain, (b ... | 1987 | 2826427 |
| immunobiological activities of a porin fraction isolated from fusobacterium nucleatum atcc 10953. | from fusobacterium nucleatum atcc 10953 cell envelope fraction whose inner membranes had been removed by treatment with sodium n-lauroyl sarcosinate, an outer membrane protein (37,000 mr in a native state) was prepared by extraction with lithium dodecyl sulfate. the protein thus obtained showed distinct porin activity, namely, the ability to form hydrophilic diffusion pores by incorporation into the artificial liposome membrane. the porin fraction exhibited strong immunobiological activities in ... | 1988 | 2831155 |
| evidence for a na+/h+ antiport in stomach smooth muscle cells. | single smooth muscle cells were isolated from circular muscle of the canine gastric corpus by collagenase incubation. cytoplasmic ph (phi) of these cells was measured fluorometrically using the trapped dye 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein. cells were examined for their na+/h+ exchange activity after intracellular acidification. cells acid-loaded by propionate exposure, the nh4+ prepulse technique or suspension in a na+-depleted medium regained almost normal phi upon exposure to a n ... | 1988 | 2831889 |
| high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen. | sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ... | 1988 | 2832429 |
| insulin-like growth factor i binding in hepatocytes from human liver, human hepatoma, and normal, regenerating, and fetal rat liver. | insulin-like growth factor-i (igf-i) in human hepatoma cells (hep-g2) has, in addition to its effect on cell growth, short-term metabolic effects acting through its own receptor. we have demonstrated that normal human hepatocytes, compared with hep-g2 cells, have virtually no igf-i binding sites. because the rate of growth is the major difference between the hepatoma and the normal liver, we asked if normal liver might express igf-i binding sites under physiologic growth conditions. indeed, wher ... | 1988 | 2832449 |
| a substituted thieno[3.4-d]imidazole versus substituted benzimidazoles as h+, k+-atpase inhibitors. | s 3337, 2-(2-ethylaminobenzylsulfinyl)-5,6-dimethoxybenzimidazole, and s 1924, 2-(5-methyl-2-picolylsulfinyl)-1h-thieno[3.4-d]imidazole, are members of new classes of h+, k+-atpase inhibitors. their effects on h+, k+-atpase and 14c-aminopyrine uptake in gastric glands were studied as well as in vivo in pylorus-ligated rats, stomach-lumen-perfused rats and heidenhain pouch dogs. their inhibitory effects were compared with the effect of omeprazole. in pylorus-ligated rats the two compounds showed ... | 1988 | 2841691 |
| ecology of colorado tick fever. | | 1988 | 2849373 |
| [role of alpha-1 and alpha-2-adrenoreceptors in achieving the compensatory response of the hemostasis system to plasmin]. | | 1988 | 2849566 |
| antiviral chemoprophylaxis for cytomegalovirus infection. | | 1988 | 2849602 |
| preparation by direct metal exchange and kinetic study of active site metal substituted class i and class ii clostridium histolyticum collagenases. | active site metal substitutions for both gamma- and zeta-collagenases from clostridium histolyticum have been made by direct metal exchange. the incubation of co(ii), cu(ii), ni(ii), cd(ii), and hg(ii) with these native collagenases results in changes in activity that parallel those observed for the reconstitution of the respective apoenzymes with these metal ions. for both collagenases, the exchange reactions with co(ii) and cu(ii) are complete within 1 min. however, the changes in activity obs ... | 1988 | 2849992 |
| myoglobin in the heart tissue of fishes lacking hemoglobin. | myoglobin has been identified in the heart tissue of three species of antarctic icefish, chaenocephalus aceratus, pseudochaenichthys georgianus and chaenodraco wilsoni. quantitative analysis shows myoglobin concentrations that are substantially lower than other teleost fish. a simple and accurate method for the direct measurement of myoglobin in tissue is described. | 1985 | 2863077 |
| [limitation of phage multiplication by microbes of the genus bordetella]. | possible causes limiting the multiplication of bordetella phages or inducing their restriction, such as the influence of lysogenic immunity and the restriction-modification (r-m) system or the incompatibility of the receptor apparatus, have been studied. the limitation of the multiplication of phages by some b. bronchiseptica and b. pertussis strains has been shown to be due to the presence of the r-m system and lysogenic immunity. in five b. bronchiseptica strains and two b. pertussis strains s ... | 1985 | 2868594 |
| [activity of cefotetan against anaerobic bacteria]. | we determined the susceptibility to antibiotics of 64 strains of obligate anaerobes. tested bacterial strains were as follows: 28 bacteroides fragilis, 22 fusobacterium nucleatum, 10 clostridium perfringens, and 4 bacteroides bivius. tested antibiotics were cefotetan, cefoxitin and piperacillin. cefotaxime was tested against 20 bacteroides fragilis strains. bacteroides fragilis, which is the most resistant species, was susceptible to cefotetan, cefoxitin and piperacillin, with comparable mics fo ... | 1986 | 2877431 |
| homocysteic acid, an endogenous agonist of nmda-receptor: release, neuroactivity and localization. | | 1986 | 2878563 |
| the challenge of malaria vaccine: trials and tribulations. | | 1987 | 2880172 |
| [prospects of improving microbial larvicides and methods of control of blood sucking insects and disease vectors]. | | 1987 | 2883565 |
| aids definition for africa. | the positive predictive values for hiv seropositivity are compared using who and us centers for disease control (cdc) clinical case definitions of acquired immunodeficiency syndrome (aids), for cases in rwanda, africa. it is postulated that the article by colebunders and co-workers should encourage clinicians and epidemiologists working in africa to adopt the world health organization's provisional clinical case definition for aids. although the predictive value for hiv seropositivity calculat ... | 1987 | 2885595 |
| efficacy of recombinant dna plasmodium falciparum sporozoite vaccine? | | 1987 | 2885674 |
| partial characterization of a peptidoglycan-protein complex from fusobacterium nucleatum fev1. | a protein in the cell wall of fusobacterium nucleatum fev1 remained associated with the peptidoglycan during extraction with various detergents and organic solvents. on digestion of this peptidoglycan-protein complex (ppc) with murein hydrolases, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) showed polypeptide bands with apparent molecular weights (mws) in the range of 3000 to 40,000. after reaction with maleic anhydride the electrophoretic mobilities of these polypeptide ... | 1987 | 2887084 |
| action of anticytoskeletal compounds on in vitro cytopathic effect, phagocytosis, and adhesiveness of trichomonas vaginalis. | the cytopathic effects of trichomonas vaginalis treated with inhibitory concentrations of anticytoskeletal compounds (mebendazole, griseofulvin, colchicine, taxol, and cytochalasin b) were studied in mouse clid fibroblast cultures. the evaluation, at different times, of cell numbers and morphological alterations showed that cytopathic effect was considerably reduced when protozoa were pretreated with mebendazole and griseofulvin, whereas colchicine, taxol, and cytochalasin b had less effect. fur ... | 1987 | 2888725 |
| desensitization of beta-adrenoceptors following repeated injections of 2-substituted 4-phenylquinolines. | the chronic effects of five 2-substituted 4-phenylquinoline derivatives on the sensitivity of the noradrenergic cyclic amp-generating system in the rat brain cortex have been determined, and compared with those of the typical and atypical antidepressants imipramine and trazodone, respectively. acute treatment (single i.p. dose of 20 mg kg-1) and sub-chronic treatment (20 mg kg-1 daily for 10 days) induced no significant desensitization of the beta-adrenoceptors. however, chronic treatment (20 mg ... | 1987 | 2890745 |
| mechanism of proline-specific proteinases: (i) substrate specificity of dipeptidyl peptidase iv from pig kidney and proline-specific endopeptidase from flavobacterium meningosepticum. | the substrate specificity of dipeptidyl peptidase iv (dipeptidyl peptide hydrolase, ec 3.4.14.5) from pig kidney and proline-specific endopeptidase from flavobacterium meningosepticum, was investigated with a series of n-terminal unprotected (dipeptidyl peptidases iv) and succinylated dipeptidyl-p-nitroanilides (proline-specific endopeptidase). both enzymes are specific for the s configuration of the amino-acid residue in p1 and p2 position if the penultimate residue is proline. in the case of a ... | 1988 | 2896517 |
| involvement of prostaglandins in histamine-induced fluid and electrolyte secretion by rat colon. | histamine increased the transmural potential difference across rat colon in-vivo and induced a net secretion of fluid. both effects were inhibited by indomethacin. histamine increased the potential difference and short-circuit current, and reduced tissue resistance in colonic sheets in-vitro. this response was reduced in the absence of chloride in the bathing medium or in the presence of serosal frusemide, suggesting that histamine stimulated electrogenic chloride secretion by the colon. the ris ... | 1988 | 2897440 |
| the effect of metoclopramide treatment on diabetic cystoparesis. | | 1988 | 2897479 |
| comparative efficacy of ceftriaxone and rifampicin in eradicating pharyngeal carriage of group a neisseria meningitidis. | during an outbreak of meningococcal meningitis in saudi arabia, oral rifampicin (four doses in two days) was compared with a single intramuscular dose of ceftriaxone for prophylaxis in family contacts of patients with meningococcal disease. pharyngeal samples were taken for culture before and 1 and 2 weeks after administration. both follow-up cultures indicated that ceftriaxone was significantly more effective. at 1 week the eradication rates for ceftriaxone and rifampicin were 97% and 75%; at 2 ... | 1988 | 2897515 |
| neurotransmitters, cytokines, and the control of alveolar bone remodeling in orthodontics. | this article described research aimed at testing the hypothesis that tissue remodeling during orthodontic tooth movement is modulated, at least in part, by factors derived from the nervous and vascular (immune) systems. specifically, the neurotransmitters sp and vip and the cytokines il-1 alpha and il-1 beta were localized immunohistochemically in paradental tissues of cat canines that had been treated by the application of an 80 g tipping force for 1 hour to 14 days. increased staining (concent ... | 1988 | 2900159 |
| importance of pulmonary blood volume and aortic blood pressure in regulation of left ventricular function. | | 1988 | 2900297 |
| localization of the mdx mutation within the mouse dystrophin gene. | we have mapped human and mouse x chromosome-specific genomic and cdna probes through an interspecies mus musculus/spretus pedigree which contains the mdx mutation. the positions of these markers relative to one another and to the mdx mutation were delineated. using probes corresponding to segments of the human duchenne muscular dystrophy (dmd) gene transcript, the position of a cross-hybridizing mouse equivalent gene (mdmd) was located. in more than 200 animals mapped, three were identified whic ... | 1988 | 2903046 |
| the pre-s2 region of hepatitis b virus: more questions than answers. | | 1989 | 2912831 |
| isolation of a corncob (coaggregation) receptor polypeptide from fusobacterium nucleatum. | corncobs, which are distinct morphological units formed by the ordered coaggregation of a filamentous microorganism and streptococci, can be made in vitro by using oral strains of fusobacterium nucleatum and streptococcus sanguis. previous studies have shown that strains of f. nucleatum contain one of at least two different types of corncob receptor. the objective of this study was to isolate the receptor from f. nucleatum atcc 10953 as the first step in the elucidation of the molecular basis of ... | 1989 | 2912893 |
| extrarenal potassium adaptation: the role of aldosterone. | 1. prior adaptation to a high potassium (hk) diet reduces the increment in plasma potassium after nephrectomy and acute potassium loading. previous work has suggested that this 'extrarenal potassium adaptation' is due to direct stimulation of cellular potassium uptake by chronic hyperaldosteronism. 2. in contrast, we have shown that when dietary potassium is withdrawn from hk rats, large urinary potassium losses persist, resulting in 'paradoxical potassium depletion'. this potassium depletion fa ... | 1989 | 2924513 |
| oral flora of mongrel and beagle dogs with periodontal disease. | the plaque flora was studied in adult mongrel and beagle dogs with periodontal disease. gingival plaque from maxillary premolars was removed and cultured on various growth media. the flora in all dogs was composed of mostly anaerobic gram negative rods. bacteroides asaccharolyticus was found in the highest proportion of plaque samples from mongrel dogs, and decomposed hydrogen peroxide suggesting catalase activity. fusobacterium nucleatum was found in higher proportion in the plaque of beagle do ... | 1989 | 2927018 |
| [occupational dermatomycoses of zoophilic origin in bulgaria]. | the relative proportion of zoophilic dermatomycoses is increased in comparison with tinea capitis et pedum. the most frequent zoophilic species now is not only t. mentagrophytes, but t. verrucosum as well. occupational mycoses caused by t. mentagrophytes and t. quinckeanum in vivaria put forward new problems for experimental medicine. the author states his arguments in favour of t. quinckeanum as a separate, specialized species with reduced morphology, forming scutula. t. verrucosum is phylogene ... | 1985 | 2933238 |
| thyroxine induced transformation in sarcoplasmic reticulum of rabbit soleus and psoas muscles. | the properties of the sarcoplasmic reticulum membranes isolated from slow-twitch type i soleus and fast-twitch type ii psoas muscles of control and thyroxine treated rabbits were comparatively studied. membrane yield, maximal calcium storing capacity, atp-supported calcium uptake, calcium-dependent atpase activity and calcium-dependent phosphoprotein formation were found to be 3-10 fold higher in psoas than in soleus preparations. membrane yield, calcium-dependent atpase activity, atp-supported ... | 1985 | 2934902 |
| oligosaccharides obtained by partial hydrolysis of lipopolysaccharide from fusobacterium nucleatum f1. | | 1986 | 2937904 |
| [abnormal location of ligula intestinalis l. in scardinius erythrophthalmus l. from lake trasimeno]. | | 1986 | 2944464 |
| the effect of phenytoin on glutamate and gaba transport. | phenytoin was observed to inhibit competitively the sodium dependent high affinity synaptosomal transport of both glutamate (glu) and gamma-aminobutyric acid (gaba) with ki values of 66 +/- 10 and 185 +/- 65 microm, respectively. this contrasted with a previous report that the uptakes of glu and gaba were enhanced by phenytoin. the degree of inhibition is dependent on the concentrations of the competing drug and substrate present. taking the therapeutic levels of phenytoin and the overall brain ... | 1986 | 2946971 |
| an ultrastructural study of nerve cell bodies in the carotid body and common carotid artery of the domestic fowl. | | 1986 | 2948419 |
| [morphologic studies following coronary thrombolysis and ptca]. | compared with those of other species human coronary arteries show a particularly marked diffuse thickening of the intima. superimposed on this diffuse thickening local sclerotic changes develop. in early stages they contain plenty of lipids which are mainly stored in modified smooth muscle cells, partly also in cells of monocytic origin. normally ruptures of arteriosclerotic plaques are responsible for thrombotic occlusions in extensive transmural myocardial infarctions. they are caused by ruptu ... | 1986 | 2950674 |
| multiple fusobacterium nucleatum liver abscesses. association with a persistent abnormality in humoral immune function. | a previously healthy 29-year-old man developed multiple hepatic abscesses secondary to fusobacterium nucleatum. no underlying local disease was found. the leading portal of entry for the bacterium may have been the oral cavity; 4 days before the onset of his illness he had had extensive dental work. immunological evaluation during the illness and in late convalescence (16 weeks after onset) revealed a persistent b cell abnormality characterized by markedly depressed in vitro secretion of immunog ... | 1987 | 2952706 |
| the effects of intravenous injection of alpha-human atrial natriuretic polypeptide on blood pressure, renal hemodynamics and urinary kinin excretion in anesthetized rabbits. | studies were performed in anesthetized rabbits to examine the effects of alpha-human atrial natriuretic polypeptide (alpha-hanp) on blood pressure, renal hemodynamics and urinary kinin excretion. intravenous bolus injection of alpha-hanp at a dose of 5 micrograms resulted in a transient increase in renal blood flow from 26.6 +/- 2.2 to 33.7 +/- 2.2 ml/min (p less than 0.05) and a decrease in mean arterial pressure from 113 +/- 1.8 to 107 +/- 2.2 mmhg (p less than 0.01). the calculated renal vasc ... | 1987 | 2953089 |
| reactive sulfhydryl groups of sarcoplasmic reticulum atpase. i. location of a group which is most reactive with n-ethylmaleimide. | ca2+-transporting atpase of rabbit skeletal muscle sarcoplasmic reticulum contains several sh groups which are reactive with n-ethylmaleimide (malnet) at ph 7.0. the location of the one which is most reactive with malnet (shn, kawakita et al. j. biochem. 87, 609 (1980)) was identified on the amino acid sequence of the atpase. shn was labeled by reacting sarcoplasmic reticulum membranes with [14c] malnet to a labeling density of 1 mol/mol atpase. [14c]malnet-labeled membranes were digested with t ... | 1987 | 2953711 |
| fusobacterium nucleatum-mediated immunomodulation of the in vitro secondary antibody response to tetanus toxoid and actinobacillus actinomycetemcomitans. | | 1987 | 2963111 |
| human milk-derived lactoferrin inhibits mitogen and alloantigen induced human lymphocyte proliferation. | purified lactoferrin (lf), isolated from human milk, was tested for its effect on human t-lymphocyte proliferative responses to phytohemagglutinin (pha) and to alloantigens in mixed lymphocyte culture. lf inhibited proliferation in both assays in a dose-dependent manner. the suppressive effect was not due to lf mediated cytotoxicity since washing cells that had been pre-incubated with lf restored their proliferative activity. lf was most effective in suppressing the pha response when added withi ... | 1987 | 2963123 |
| oestrus phases of the syrian hamster. | | 1988 | 2976222 |
| free radicals and myocardial ischemia. the role of xanthine oxidase. | recent studies have established a major role for oxygen-derived free radicals in post ischemic tissue injury to the intestine. during ischemia, there appears to be a calcium-triggered, protease-dependent conversion of the native xanthine dehydrogenase to a superoxide-producing xanthine oxidase. the catabolic degradation of atp during ischemia provides an oxidizable substrate, hypoxanthine. on reperfusion, molecular oxygen is resupplied and a burst of superoxide production ensues, resulting in ex ... | 1985 | 2982206 |
| modification of neurotransmitter receptor sensitivity in cat visual cortex during the critical period. | we have examined the characteristics of various receptors in cat visual cortex during postnatal development. these included beta-adrenergic, gaba, benzodiazepine and acetylcholine receptors. for each population of receptor the number (bmax) and affinity (kd) were examined as a function of postnatal age (3 days-adult). for all receptors examined, the bmax increased during development from low early values to a peak within the critical period. the kd also changed during development for most recept ... | 1985 | 2994853 |
| expression of amplified human beta interferon genes using heavy metal induction in chinese hamster ovary cells. | an expression cassette consisting of the human beta-interferon (beta-ifn) cdna fused to the human metallothionein (met)iia promoter has been linked to a selectable mouse dihydrofolate reductase gene (dhfr) and used to transform dhfr-deficient chinese hamster ovary (cho) cells. transformants resistant to increasing concentrations of methotrexate (mtx) were isolated and found to secrete beta-ifn either constitutively or upon induction with cadmium (up to 325 000 units beta-ifn/10(6) cells/24 h). m ... | 1985 | 2996984 |
| nucleotide sequence of the paer7 restriction/modification system and partial characterization of its protein products. | bal31 deletion experiments on clones of the paer7 restriction-modification system from pseudomonas aeruginosa demonstrate that it is arranged as an operon, with the methylase gene preceding the endonuclease gene. the dna sequence of this operon agrees with in vitro transcription-translation assays which predict proteins of 532 amino acids, mr = 59,260 daltons, and 246 amino acids, mr = 27,280 daltons, coincident with the methylase and endonuclease genes, respectively. these predicted values coin ... | 1985 | 3001639 |
| platelet-activating factor-binding protein in human serum. | the occurrence of a kind of protein (pbp) with stronger paf-binding ability than albumin was shown in human serum. the molecular weight of pbp was approximately 160k to 180k. pbp seemed to be a pentamer composed of five 32k subunits. paf bound to pbp revealed platelet aggregation and was resistant to acetyl-hydrolase. paf synthesized in neutrophils stimulated with the ionophore a23187 was released by pbp from cells. | 1985 | 3004155 |
| a novel type of human papillomavirus associated with genital neoplasias. | the role of human papillomaviruses (hpvs) in the development of genital neoplasias has been well documented. the genomes of two hpv types, hpv16 and hpv18, have been found to be associated with about 70% of invasive carcinomas of the uterine cervix. as, under non-stringent hybridization conditions, hpv dna sequences have been detected in about 90% of cervical carcinomas, it seems likely that additional hpv types are associated with these tumours. here we report the molecular cloning and characte ... | 1986 | 3012352 |
| a second post-transcriptional trans-activator gene required for htlv-iii replication. | evidence is provided for the existence of a seventh gene in the genome of human t-lymphotropic virus type iii/lymphadenopathy-associated virus. the gene is necessary for replication and acts post-transcriptionally to relieve negative regulation of the messenger rna for the virion capsid and envelope proteins. these observations suggest mechanisms for regulating both the latent and lytic phases of the virus life cycle. | 1986 | 3012355 |
| coagglutination reactions between candida albicans and oral bacteria. | an agglutination assay for detecting intermicrobial adherence between the cells of candida albicans and various oral bacteria is described. strains of streptococcus sanguis, s. salivarius, s. mutans, s. mitis, fusobacterium nucleatum and actinomyces viscosus all coagglutinated with c. albicans. no interaction could be demonstrated between the cells of bacteroides melaninogenicus and those of c. albicans. preliminary investigations of these interactions suggest that binding of f. nucleatum and a. ... | 1986 | 3018251 |
| chromosome damage induced by herpes simplex virus type 1 in early infection. | the chromosome damage induced by herpes simplex virus type 1 (hsv-1) in vitro was examined up to 6 h after infection using ht-1080 cells. initial damage occurring within 3 h was specific, involving uncoiling of chromosome 1q12-21 and to a lesser extent the pericentric regions of chromosomes 9, 16 and satellited chromosomes. for the initial unwinding, synthesis of the immediate early class of hsv proteins needed to occur as was demonstrated using hsv-1 temperature-sensitive mutants tsk and tsb7 a ... | 1986 | 3020163 |
| [nuclear magnetic resonance spectroscopy in medicine: biochemistry of intact tissues]. | | 1986 | 3020443 |
| [nuclear magnetic resonance spectroscopy in medicine: biochemistry of intact tissues]. | | 1986 | 3020443 |
| a computer-aided analysis of the effect of peripheral nerve transection on tmpase activity of substantia gelatinosa rolandi. | | 1986 | 3020818 |
| methylcholanthrene: a possible pseudosubstrate for adrenocortical 17 alpha-hydroxylase and aryl hydrocarbon hydroxylase. | in cultured bovine adrenocortical cells, loss of 17 alpha-hydroxylase activity was observed after incubation with 3-methylcholanthrene (3-mc). the suppression of 17 alpha-hydroxylase by 3-mc was rapid (50% loss of activity in 10 hr at 1 microm 3-mc), did not exhibit a lag period, and was not affected by cycloheximide. direct effects of 3-mc on 17 alpha-hydroxylase were observed only at high concentrations, but the concentration for 50% loss of activity was 0.3 microm when 3-mc was added for 24 h ... | 1986 | 3021165 |
| regulation of guinea-pig immune functions by interleukin 2: critical role of natural killer activity in acute hsv-2 genital infection. | we have previously demonstrated that recombinant interleukin 2 (ril 2) has a protective effect against acute hsv-2 infection in guinea pigs with a biphasic dose response which peaked between 4 and 20 x 10(4) u/kg, whereas 8 x 10(5) u/kg showed no effect on disease. animals that escaped infection appeared lack immunologic memory to hsv-2, suggesting a nonspecific immune mechanism. in this study we have found that nk activity of fresh splenocytes measured against hsv-2 infected human foreskin fibr ... | 1986 | 3021853 |
| detection of dihydroorotate dehydrogenase activity as a marker of de novo nucleic acid synthesis in germinal centers of spleens following antigen administration. | a histochemical technique, developed for the localization of dho-d activity in tissue, has been utilized to study the distribution of this activity in rabbit spleen. this enzyme is involved in the de novo synthesis of pyrimidines of nucleic acids via orotic acid as an intermediate. the enzyme was found to be most active at the periphery of follicles in the resting spleen. following antigenic challenge, enzyme activity became prominent for several days in macrophages within germinal centers, but ... | 1985 | 3023585 |
| characterization of benzodiazepine receptors in the bovine pineal gland: evidence for the presence of an atypical binding site. | bovine and rat pineal benzodiazepine receptors were characterized using ligands with high affinities for either 'central-type' (cbr) or 'peripheral-type' (pbr) benzodiazepine receptors. the characteristics (bmax = 83 +/- 10 fmol/mg protein, kd = 3.88 +/- 0.46 nm) of benzodiazepine receptors in bovine pineal membranes measured with [3h]flunitrazepam (using flunitrazepam to define non-specific binding) were consistent with previously reported values. however, if non-specific binding was defined us ... | 1986 | 3024779 |
| characterization of benzodiazepine receptors in the bovine pineal gland: evidence for the presence of an atypical binding site. | bovine and rat pineal benzodiazepine receptors were characterized using ligands with high affinities for either 'central-type' (cbr) or 'peripheral-type' (pbr) benzodiazepine receptors. the characteristics (bmax = 83 +/- 10 fmol/mg protein, kd = 3.88 +/- 0.46 nm) of benzodiazepine receptors in bovine pineal membranes measured with [3h]flunitrazepam (using flunitrazepam to define non-specific binding) were consistent with previously reported values. however, if non-specific binding was defined us ... | 1986 | 3024779 |
| [immunobiological activity of 41k protein (porin) derived from the cell envelope of fusobacterium nucleatum]. | | 1986 | 3025315 |
| ro 15-1788 suppresses the development of kindling through the benzodiazepine receptor. | beta-carboline (norharman) has been shown to produce kindled seizures when given systemically for long periods of time. the expression of the kindled seizure activity can be blocked by ligands of the benzodiazepine receptor suggesting the receptor as a site of vulnerability in the kindling mechanism. the present data show that ro 15-1788, a receptor antagonist, suppresses the development of kindled seizures as demonstrated by the delayed appearance of each behavioral stage and the decreased seve ... | 1986 | 3026559 |
| primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen. | the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ... | 1986 | 3028680 |
| primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen. | the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ... | 1986 | 3028680 |
| identification of a novel herpes simplex virus type 1-induced glycoprotein which complexes with ge and binds immunoglobulin. | we detected a glycoprotein on the surface of cells infected with herpes simplex virus type 1 (hsv-1) which, in conjunction with ge, binds immunoglobulin g (igg). the novel glycoprotein, which has an apparent molecular mass of 70 kilodaltons and was provisionally named g70, was first detected in extracts of hsv-1-infected cells labeled by lactoperoxidase-catalyzed iodination and precipitated with rabbit sera or igg and protein a-sepharose. in subsequent experiments, g70 and ge were coprecipitated ... | 1987 | 3035221 |
| herpes simplex virus immediate-early promoters are responsive to virus and cell trans-acting factors. | the promoters for each of the immediate-early genes from herpes simplex virus type 1 were cloned and fused to a chloramphenicol acetyltransferase cassette. these chimeric genes were used as targets in a transient expression assay to determine how the immediate-early gene products icp4 and icp0 and the virion-associated stimulatory protein vmw65 affected their expression in hela and vero cells. the basal level of expression from these cassettes differed significantly depending on the extent of 5' ... | 1987 | 3035226 |
| sv40-based escherichia coli shuttle vectors infectious for monkey cells. | we describe sv40-based escherichia coli shuttle vectors which can be packaged as pseudovirions without excision of plasmid sequences and which can be rescued in bacteria. these vectors replicate and are transmitted as virus in monkey cos cells without requiring a helper virus. extrachromosomal vector dna isolated from infected cells can be rescued in e. coli, so that dna alterations can be easily screened. indeed, some of the constructions give rise to very stable plasmids with no detectable rea ... | 1987 | 3036664 |
| source of iron in neutrophil-mediated killing of endothelial cells. | recently we have shown that human neutrophils activated with phorbol ester are cytotoxic for cultured bovine pulmonary artery endothelial cells in an iron-dependent manner. by using the ferric iron chelator deferoxamine mesylate, we have now investigated the source of the iron. pretreatment of neutrophils with deferoxamine mesylate affected neither their production of o2- nor their cytotoxicity for endothelial cells after addition of phorbol ester. however, similar pretreatment of endothelial ce ... | 1987 | 3037192 |
| production of immunoreactive growth hormone by mononuclear leukocytes. | in the present study, we evaluated whether mononuclear leukocytes could synthesize and secrete growth hormone (gh) in vitro. by using rna slot blot analysis, we detected maximum spontaneous levels of specific gh mrna in the cytoplasm of rat leukocytes after a 4-h incubation. northern gel analysis demonstrated that the specific leukocyte gh rna was polyadenylated and had a molecular mass of 1.0 kb. further studies using immunofluorescence, antibody affinity chromatography, and sephacryl gel filtr ... | 1988 | 3044906 |
| [mass-spectrometric determination of specificity of proteinases]. | analysis of methods of the proteinase substrate specificity determination revealed that michaelis constants found from the rates of hydrolysis of small synthetic substrates cannot be a good measure of selectivity, especially for proteinases of broad specificity used for hydrolysis of substrates of high molecular mass with multiple cleavage sites. mass-spectrometric studies (eriad) of the hydrolysis of the insulin b chain with trypsin, chymotrypsin, and proteinase i showed that monitoring of the ... | 1988 | 3056418 |
| [cryopreservation of early human embryo stages]. | a short review of freezing procedures applied to early human embryos is given. it is noted that human embryos survived freezing and thawing at a developmental stage of 1. cell to blastocyst. but it seems to be necessary to use for any developmental stage of early embryo a special freezing and thawing method. embryo survival is correlated with their morphologic features where as neither age of embryos nor developmental stage were involved in freezing and thawing ability. | 1988 | 3071049 |
| regional localization of cd18, the beta-subunit of the cell surface adhesion molecule lfa-1, on human chromosome 21 by in situ hybridization. | the gene coding for the beta-subunit of the cell surface adhesion glycoprotein lfa-1 has been localized to the tip of the long arm of chromosome 21 at 21q22.1-qter. | 1988 | 3073708 |
| localization of the kras2 oncogene in the domestic rabbit (oryctolagus cuniculus). | the kras2 gene was localized in the rabbit by chromosomal in situ hybridization, using a 3h-labeled human cdna probe. there were 201 silver grains on 144 metaphase spreads; 12.9% of the grains resided on chromosome 16, and 54% of these grains were located close to the centromere at 16p11----q11. statistical analysis indicated that labeling at this region represents a significant deviation from a random distribution and thus provided evidence for the assignment of kras2 to 16p11----q11. in additi ... | 1988 | 3073912 |
| anaerobic infections in the head and neck region. | anaerobic bacteria form the predominant flora of the oral cavity, outnumbering facultative organisms by 10-1,000: 1. the type of anaerobic bacteria and their concentration depend on the anatomical site and the degree of anaerobiosis in the different sites in the mouth. three groups of anaerobic bacteria inhabit the oral cavity; the strict anaerobes, the moderate anaerobes, and the microaerophilic group of organisms. the majority of anaerobic bacterial infections occurring in the region of the mo ... | 1988 | 3074469 |
| specificity and retinotectal projections of quarter-eye fragments in xenopus laevis. | three quarters of the eye anlage in xenopus embryos of stage 33/34 were eliminated in three different sets of experiments. the remaining quadrant originated from the nasoventral part of the retina, from its ventral portion, or from the temporo-ventral area of the retina. all the fragments developed into small eyes of normal shape. the retinotectal connections did not deviate from those found in the control groups, even though mirror-image duplication was fairly frequent. for all fragments the te ... | 1988 | 3077005 |
| 1,25 dihydroxyvitamin d increases hepatocyte cytosolic calcium levels. a potential regulator of vitamin d-25-hydroxylase. | 1,25 dihydroxyvitamin d (1,25(oh)2d) has been demonstrated to inhibit hepatic 25 hydroxyvitamin d (25 ohd) production. changes in cytosolic calcium have been shown to regulate cellular processes. using the fluorescent dye quin 2, we have investigated the effects of 1,25(oh)2d and 24,25(oh)2d on cytosolic calcium levels in hepatocytes. 1,25(oh)2d exposure for 5 min increases cytosolic calcium levels by 24% at a concentration of 100 pg/ml, 39% at a concentration of 1 ng/ml, and 50% at a concentrat ... | 1986 | 3084563 |
| chemiluminescence in the assessment of polymorphonuclear leukocyte function in chronic inflammatory periodontal disease. | polymorphonuclear neutrophils (pmn's) constitute the primary host resistance factor against infection. they are prominent cells in both the gingival tissue and gingival sulcus in most forms of periodontal disease. although defective pmn function has been implicated in the pathogenesis of localized juvenile periodontitis (ljp) and rapidly progressive periodontitis (rpp), this may not necessarily be the case in adult periodontitis (ap). a number of studies have failed to detect pmn dysfunction in ... | 1986 | 3084735 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |