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positive and negative associations between bacterial species in dental root canals.significant associations have been previously reported between certain pairs of bacterial species isolated from human dental root canals. the aim of this study was to examine microbiologically a more extensive series of cases, with particular reference to obligate anaerobes which accounted for 64% of total isolations. a total of 65 different species was isolated and individual root canals yielded a maximum of eleven bacterial species. highly significant positive associations (p < 0.001) were fou ...19947700162
evaluation of the etest for antimicrobial spectrum and potency determinations of anaerobes associated with bacterial vaginosis and peritonitis.one hundred ninety-seven anaerobic organisms (24 gardnerella vaginalis, 16 mobiluncus spp., 19 peptostreptococcus spp., 20 lactobacillus spp., 20 prevotella bivia/disiens, 81 bacteroides fragilis group, 12 clostridium spp., and five fusobacterium spp.) were processed by the etest (ab biodisk, solna, sweden) and a reference (brucella blood agar) method against 10 antimicrobial agents. for the bacterial vaginosis-associated pathogens, the etest was more reproducible and correlated acceptably with ...19947705035
the effect of plaque control in subjects with shallow pockets and high prevalence of periodontal pathogens.in a previous study, it was shown that professional tooth cleaning 3x a week had a significant influence on the subgingival microbiota of shallow pockets. the purpose of this investigation was to study the effect of a single episode of full-mouth supragingival cleaning and oral hygiene instructions in subjects with minimal periodontal disease but high prevalence of putative periodontal pathogens. 10 subjects from arabic countries, aged between 22 and 48 years, which had previously not been expos ...19957706543
three-year tooth loss among black and white older adults in north carolina.the distribution and determinants of tooth loss in older adults are poorly defined, especially in blacks, who have been underrepresented in previous studies. this study investigated, epidemiologically, the distribution and predictors of tooth loss in elder blacks and whites by following a random sample of older adults in north carolina for three years. it was hypothesized that blacks would be at greater risk of tooth loss and would have different risk factors for tooth loss. data from 263 blacks ...19957722064
predominant microflora of severe, moderate and minimal periodontal lesions in young adults with rapidly progressive periodontitis.the purpose of this investigation was to study the microflora of severe, moderate and minimal periodontal lesions, in young adults with rapidly progressive periodontitis (rpp). subgingival plaque samples were taken from 142 periodontal lesions in 10 young adults aging 25 to 35 years. the examination of the subgingival microflora indicated that certain species, including porphyromonas gingivalis, bacteroides forsythus, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, and campylobact ...19957722848
microbiology of chronic suppurative otitis media in children in surabaya, indonesia.the aerobic and anaerobic microbiology of 38 children from surabaya, indonesia, who suffered from chronic suppurative otitis media (csom) was studied using strict microbiological methodology. a total of 106 isolates (49 anaerobic and 57 aerobic) were recovered. aerobic organisms alone were isolated from 11 (29%), anaerobic bacteria only in 4 (11%) and mixed aerobic and anaerobic flora were present in 23 (60%). the predominant organisms were peptostreptococcus sp., prevotella sp., bacteroides sp. ...19957729990
detection and incidence of the tetracycline resistance determinant tet(m) in the microflora associated with adult periodontitis.subgingival plaque samples were collected from 68 patients with adult periodontitis, enumerated on trypticase-soy blood agar plates, with and without tetracycline at 4 micrograms/ml, and incubated anaerobically for 5 days. each different colony morphotype was enumerated, and a representative colony was subcultured for identification and examined for the tetracycline resistance gene tet(m). both pcr amplification and dna hybridization, using a fragment of tet(m) from tn1545, were used to detect t ...19957730961
bacterial-stimulated cytokine production of peripheral mononuclear cells from patients of various periodontitis categories.periodontitis is a general term for disease categories, including juvenile periodontitis (jp), rapidly progressive periodontitis (rpp), and adult periodontitis (ap), which may or may not share a common etiology and pathogenesis. these disease categories are characterized by differences in progression of tissue destruction and differences in age group susceptibility, but not, to our knowledge, by differences in cytokine responses of inflammatory cells. the present study examined blood cell counts ...19957730965
[characterization of microflora in pyo-destructive lung diseases].the microflora pattern of the endobronchial secretion was investigated in 74 patients with purulent destructive diseases of the lungs. specimens of the endobronchial secretion were collected by transtracheal aspiration and bronchoalveolar lavage. the anaerobic isolates were cultivated in anaerostats (oxoid, england) with the use of gaspacks. bacteriological identification of the pathogens was carried out with respect to 67 patients (90.5 per cent). non-sporulating anaerobic microflora was isolat ...19947733776
sensitivity of porphyromonas and prevotella species in liquid media to argon laser.the phototoxicity of argon laser irradiation was studied in aqueous suspensions of porphyromonas endodontalis (american type culture collection [atcc] 35406), porphyromonas gingivalis (atcc 33277), prevotella denticola (atcc 33184) and two strains of prevotella intermedia (atcc 15033 and 49046), all "black-pigmented bacteria," bpb, that accumulate cellular porphyrins. several of these species have been implicated in the etiology of periodontal disease. non-black-pigmented bacteria were also stud ...19957740086
surgical sepsis: constancy of antibiotic susceptibility of causative organisms.it is well documented that antibiotic therapy exerts selective pressure on bacteria. conversion of bacteria from susceptible to resistant to antibiotics has been observed often during antimicrobial therapy. it has been postulated that human intestinal reservoirs facilitate communication of transposons that can transfer resistance determinants among various bacterial species.19957740421
inhibitory effect of lactoferrin on the adhesion of actinobacillus actinomycetemcomitans and prevotella intermedia to fibroblasts and epithelial cells.adhesion of the periodontitis-associated bacteria actinobacillus actinomycetemcomitans and prevotella intermedia to monolayers of fibroblasts, hep-2, kb and hela cells was quantified with radiolabeled bacteria. bacterial adhesion was also examined microscopically with giemsa-stained non-radioactive preparations. the degree of bacterial adherence was dependent on the growth phase of the bacteria. strains at the exponential phase adhered to a greater extent than those at the stationary phase of gr ...19957748540
associations of specific bacteria with some endodontic signs and symptoms.although a wide range of bacterial species has been isolated from infected dental root canals it remains necessary to determine whether any particular group of such bacteria is associated with specific endodontic symptoms and clinical signs. in this study 30 root canals were examined microbiologically; of these, 14 were associated with pain, 20 with tenderness to percussion, 23 presented with wet root canals, seven with swelling, five with purulent exudate and four with a sinus. clinical and mic ...19947751061
prevotella and porphyromonas infections in children.from 1974 to 1994, 504 isolates of prevotella and porphyromonas spp. were obtained from 435 (21%) of 2033 specimens from 418 children. they included 160 (32%) pr. melaninogenica, 105 (21%) pr. intermedia, 84 (17%) p. asaccharolytica, 58 (12%) pr. orisbuccae, and 58 (12%) pr. oralis. most prevotella and porphyromonas spp. were isolated from abscesses (176), pulmonary infections (85), ear infections (82), wound infections (44), peritonitis (38), paronychia (15) and chronic sinusitis (14). predispo ...19957752213
breakdown of different peptides by prevotella (bacteroides) ruminicola and mixed microorganisms from the sheep rumen.several di-, tri-, and oligopeptides were incubated individually in vitro with rumen fluid from two sheep receiving a mixed grass hay/concentrate diet and with washed cells of prevotella (formerly bacteroides) ruminicola m384 and p. ruminicola b(1)4. the rates of breakdown of most peptides were similar in the rumen fluid from the two sheep. acidic and proline-containing peptides tended to be more slowly degraded than neutral or basic peptides. the dipeptide at the n-terminus of higher peptides w ...19937763640
analyses of the gene and amino acid sequence of the prevotella (bacteroides) ruminicola 23 xylanase reveals unexpected homology with endoglucanases from other genera of bacteria.the dna sequence for the xylanase gene from prevotella (bacteroides) ruminicola 23 was determined. the xylanase gene encoded for a protein with a molecular weight of 65,740. an apparent leader sequence of 22 amino acids was observed. the promoter region for expression of the xylanase gene in bacteroides species was identified with a promoterless chloramphenicol acetyltransferase gene. a region of high amino acid homology was found with the proposed catalytic domain of endoglucanases from several ...19937763664
inactivation of anaerobic bacteria by various photosensitized porphyrins or by hemin.the photodynamic effects of deuteroporphyrin (dp), hematoporphyrin derivative (hpd), hematoporphyrin (hp), or protoporphyrin (pp) on a variety of anaerobic microorganisms were examined in this study. the majority of the species, among the 350 strains tested, were inhibited by concentrations of < or = 2.5 micrograms/ml of light-activated dp. species found to be resistant to this treatment included bilophila wadsworthia, fusobacterium mortiferum, fusobacterium varium, and bacteroides gracilis. the ...19947765091
[the prospects for using the new macrolide antibiotic azithromycin (sumamed) in the combined treatment of periodontitis].antibacterial activity of sumamed, a macrolide antibiotic, in respect of anaerobic microflora of the oral cavity, such as bacteroides, prevotella, porphyromonas, fusobacterium, peptostreptococcus, str. sanguis and staph. aureus, was assessed. the drug was found to be highly effective in respect of bacteroids (mpc90: 1,4 to 30 mg/mlmg/ml, this permitting the use of sumamed in the treatment of 9 patients with chronic generalized periodontitis in the exacerbation stage. bacteriological and clinical ...19957770869
the microbiota of osseointegrated implants in patients with a history of periodontal disease.the purpose of this study was to determine the presence of suspected periodontal pathogens in the peri-implant microflora of osseointegrated implants exposed 3 and 6 months to the oral environment of patients previously treated for periodontal disease. subgingival microbial samples were taken in the deepest residual pocket of each quadrant in 10 patients before placement of iti implants and in 10 patients before abutment connection of brånemark implants. the samples were cultured using continuou ...19957775668
periodontal findings in spouses. a clinical, radiographic and microbiological study.clinical, radiographic and microbiological examination of periodontal conditions was carried out in 2 groups of married couples to assess similarities between husband and wife. the diseased probands (n = 10) exhibited advanced periodontitis and the healthy ones (n = 10) were periodontally normal. the clinical examination comprised the assessment of plaque, probing pocket depths, gingival bleeding on probing, suppuration, supragingival and subgingival calculus. the extent and type of alveolar bon ...19957775676
bacterial steroidogenesis by periodontal pathogens and the effect of bacterial enzymes on steroid conversions by human gingival fibroblasts in culture.studies were performed to investigate the effect of microbial culture supernatants of periodontal pathogens on the metabolism of radiolabelled testosterone in the presence or absence of human gingival fibroblasts. subgingival plaque samples were obtained on paper points from 3 sites with probing depth values of 6-8 mm. samples were incubated with 14c-testosterone for 24 h in brain heart infusion (bhi) broth. similar incubations were also carried out with strains of a. actinomycetemcomitans, p. i ...19957776153
succession of putative peri-implant pathogens after root-form and plate-form implant placement in partially dentate adult monkeys.this report describes the succession of putative peri-implant pathogens in partially dentate monkeys after dental implantation and prosthetic reconstruction. tooth and implant (6 root-end form, 4 blade-vent implants) sites in eight monkeys were monitored microbiologically and clinically during the pre-implant stage, abutment connection stage, bridge placement stage, and three and six months after the bridge placement stage. tooth and implant sites were cleaned monthly post-extraction. microbiolo ...19957776158
role of anaerobic bacteria in chronic otitis media and cholesteatoma.otitis media (om), a common infection in children, can cause significant morbidity. selection of the most appropriate treatment regimen directed against the pathogens responsible for the om can minimize complications. the most frequently isolated bacteria from chronic om are staphylococcus aureus, pseudomonas aeruginosa and anaerobic bacteria. the predominant anaerobes are peptostreptococcus spp., pigmented prevotella and porphyromonas spp., bacteroides spp. and fusobacterium spp. many of the or ...19957782173
[antimicrobial activities of piperacillin against fresh clinically isolated strains].in order to evaluate the antimicrobial activity of piperacillin (pipc), along with control agents, minimum inhibitory concentrations (mic's) were determined against fresh clinically isolated strains from january to june, 1994. 1. the mic70's of pipc against major strains were approximately equal to those reported in the mid 1980s. 2. strains for the study were supplied in approximately equal numbers from community-acquired hospitals and general hospitals. the ratios of bacteria resistant to beta ...19957783320
characterization of the human immunoglobulin g fc-binding activity in prevotella intermedia.many pathogenic bacteria possess cell surface receptors which can bind immunoglobulins via the fc portion. the aim of this study was to characterize the human immunoglobulin g (igg) fc-binding activity of prevotella intermedia, a suspected etiologic agent of adult chronic periodontitis. the fc-binding activity of p. intermedia on whole cells and on extracellular vesicles was demonstrated. incubation of p. intermedia cells in the presence of zwittergent 3-14 allowed complete solubilization of the ...19957790101
periodontopathogens in elderly chinese with different periodontal disease experience.if an etiological relationship exists between destructive periodontal disease and putative periodontopathogens, they would be expected to have a very low prevalence in periodontally healthy elderly persons. to test this hypothesis, 2 subgroups of elderly, rural chinese (a periodontally "best" and a "worst" group, each comprising 15 persons) were identified in 1990 from a cohort aged 55-69 years, examined in 1984. assessment of changes in periodontal status over the 6-year period were possible by ...19957790524
properties of alkaline phosphatase in the gingival crevicular fluid.the isoenzymic properties of the alkaline phosphatase (alp) of the gingival crevicular fluid (gcf) were investigated and compared with those in other cells, such as human polymorphonuclear leukocytes (pmns), and human periodontal ligament cells (pdls), and with those of three species of periodontopathic bacteria: porphyromonas gingivalis 381 (p. gingivalis), prevotella intermedia atcc25611 (p. intermedia), and capnocytophaga sputigena atcc33123 (c. sputigena). the biochemical properties of the i ...19957796488
early evaluation of necrotizing fasciitis with use of ct.gas bubbles are one of the important signs in the clinical diagnosis of necrotizing fasciitis, and radiographs are mandatory. an instance where gas bubbles not found on the plain radiographs were clearly shown by ct, which is a useful method for close monitoring and decompression of gas bubbles situated deeply in the spaces of the maxillo-facial and neck regions.19947798356
occurrence and identity of proteolytic bacteria in adult periodontitis. 19947799216
the prevalence of a. actinomycetemcomitans, p. gingivalis and p. intermedia in selected subjects with periodontitis.the present study was carried out in a private periodontal practice. 8 clinical criteria were chosen to select patients for microbiological examination. these criteria characterize prominent features of disease which at the start, during, or in the maintenance phase of treatment suggest difficulties in the progress of the periodontal therapy. based on these clinical characteristics, an inventory was made of the prevalence of the 3 putative periodontal pathogens: actinobacillus actinomycetemcomit ...19947806673
periodontal regeneration of human infrabony defects (v). effect of oral hygiene on long-term stability.the aim of this investigation was to assess the role of supportive periodontal care in the maintenance of clinical attachment gained, after surgical treatment according to the principles of gtr, in deep infrabony defects. following gtr treatment, 40 deep infrabony defects in 23 patients gained 4.1 mm of probing attachment level (pal) after 1 year of stringent plaque control. in the subsequent 3 years, 15 patients (22 sites, group a) were recalled every 3 months. in this group, the gained attachm ...19947806677
transmission of oral prevotella melaninogenica between a mother and her young child.most likely, young children acquire their oral microflora by frequent transfer of bacteria between family members. the possible transmission of obligately anaerobic prevotella melaninogenica recovered from 11 mother-child pairs was examined by ribotyping. one to 18 isolates (mean 13) per child from different oral sampling sites and 4 to 17 (mean 10) isolates per mother from stimulated salivary samples, collected on 2 occasions, were analyzed. on sampling, the mean ages of the children were 4 mon ...19947808775
genital flora in pregnancy and its association with intrauterine growth retardation.a study of risk factors for intrauterine growth retardation (iugr) was conducted among a cohort of 13,914 pregnant women enrolled in the multicenter vaginal infections and prematurity study. from 23 through 26 weeks of gestational age, cultures of specimens from the vagina and cervix were done for group b streptococci, neisseria gonorrhoeae, chlamydia trachomatis, trichomonas vaginalis, candida albicans, gardnerella vaginalis, mycoplasma hominis, ureaplasma urealyticum, and anaerobic gram-negati ...19947814541
effect of local metronidazole application on periodontal healing following guided tissue regeneration. microbiological findings.the bacteriological colonization of healing periodontal defects was investigated after treatment with guided tissue regeneration using expanded polytetrafluoroethylene membranes together with local metronidazole gel (25%, 250 mg/g). twelve patients, each with one pair of comparable defects, had the test defect treated with the membrane plus metronidazole gel and the control defect treated with the membrane alone. thirty weeks after removal of the membrane, the median gain in probing attachment l ...19947823273
in vitro activity of du-6859a against anaerobic bacteria.the activity of a new quinolone agent, du-6859a, against 330 strains of anaerobic bacteria was determined by using the national committee for clinical laboratory standards-approved wadsworth brucella laked blood agar method; the activity of du-6859a was compared with those of amoxicillin-clavulanate (2:1), chloramphenicol, ciprofloxacin, clindamycin, fleroxacin, imipenem, lomefloxacin, metronidazole, sparfloxacin, and temafloxacin. du-6859a and chloramphenicol inhibited all of the isolates at co ...19947840599
increased in vitro activity of ceftriaxone by addition of tazobactam against clinical isolates of anaerobes.a total of 461 clinical strains of anaerobes were tested using a broth microdilution test to determine the activity of the combination of ceftriaxone and tazobactam and other antimicrobials against these isolates. ceftriaxone was combined with tazobactam in ratios of 1:1, 2:1, 4:1, and 8:1 and twofold dilutions of ceftriaxone in constant concentrations to tazobactam of 2, 4, 8, 16, and 32 micrograms/ml. against beta-lactamase-producing strains of the bacteroides fragilis group, b. capillosus, an ...19947851086
cyclic amp in ruminal and other anaerobic bacteria.an examination of camp levels in predominant species of ruminal bacteria and other anaerobic bacteria was conducted. cellular camp concentrations of glucose-grown cultures of butyrivibrio fibrisolvens 49, prevotella ruminicola d31d, selenomonas ruminantium hd4 and d, megasphaera elsdenii b159, streptococcus bovis jb1, bacteroides thetaiotaomicron 5482, and clostridium acetobutylicum atcc 824 were determined at various times during growth by a competitive binding radioimmunoassay procedure. the r ...19947851742
a longitudinal study from prepuberty to puberty of gingivitis. correlation between the occurrence of prevotella intermedia and sex hormones.a longitudinal study of 24 subjects progressing normally from prepuberty to puberty was undertaken to evaluate the effects of sex hormone levels on clinical and microbiologic parameters and on serum antibodies. during elementary school, at the beginning of the longitudinal monitoring, 2 groups, 12 subjects with gingivitis and 12 gingivitis-free subjects, were selected and observed through puberty. bone ages and self-assessment of secondary sex characteristics were used to confirm puberty. a stat ...19947852609
some microbiological, histopathological and immunohistochemical characteristics of progressive periodontal disease.the aim of the present investigation was to study the local nature of human periodontal disease by assessing the microbiota and the composition of the tissue lesions at sites with progressive attachment loss in periodontitis susceptible subjects. 300 subjects with periodontal disease were monitored for 2 years without treatment. 8 subjects lost > 2 mm of attachment at > or = 3 sites during both the first and the second 12 month interval. these 8 subjects (progressive disease group; pd) were reca ...19947852619
mechanism of irrigation effects on gingivitis.although gingivitis is initiated by plaque and plaque removal controls gingivitis, gingival irrigation with water has been shown to reduce gingivitis without reducing plaque. this study attempted to explore possible mechanisms involved in the treatment of gingivitis by water irrigation. patients (n = 125) with more than 20 teeth, less than 4 sites with probing depth (pd) deeper than 6 mm, bleeding on probing (bop) frequency of 30% or higher, and no systemic disease were randomized to one of four ...19947853124
effect of treatment on some periodontopathogens and their antibody levels in periodontal abscesses.twenty patients suffering from abscess of periodontal origin were treated and followed clinically and microbiologically for 6 months. microbiological examination was performed by culture technique. one deep and one shallow periodontal pocket in the same patient were examined concomitantly. serum was collected and analyzed for specific antibody level using elisa methodology. treatment included supragingival scaling, drainage, and irrigation of the periodontal pocket with 0.85% sodium chloride and ...19947853125
comparative detection frequency of 6 putative periodontal pathogens in sudanese and norwegian adult periodontitis patients.twenty-five sudanese and 18 norwegian adult periodontitis patients were selected to participate in this study. the purpose was to compare cultivation results of porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, fusobacterium nucleatum, and capnocytophaga species as well as various enteric rods in both populations. in addition, dna probe analysis was used to identify p. gingivalis, p. intermedia, a. actinomycetemcomitans, f. nucleatum, and bacteroides forsythu ...19947853128
microbial associations of 4 putative periodontal pathogens in sudanese adult periodontitis patients determined by dna probe analysis.the few previous cultivation studies on the in vivo associations between various periodontal microbial species have shown several positive and negative associations. the present investigation utilized dna probe analysis to examine possible in vivo associations between the periodontal pathogens porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum, and bacteroides forsythus in subgingival plaque samples obtained from 25 sudanese untreated adult periodontitis patients. the stand ...19947853129
clinical and microbiological effects of controlled-release locally delivered minocycline in periodontitis.the clinical efficacy of minocycline in a subgingival local delivery system was evaluated alone (m) or as an adjunct to scaling and root planing (m + srp), in comparison to scaling and root planing (srp) or to no subgingival treatment (notx) in adult periodontitis. fifty-one adult patients with > or = 7 mm periodontal pockets demonstrating the presence by culture of porphyromonas gingivalis (pg), prevotella intermedia (p(i)), or actinobacillus actinomycetemcomitans (aa) were randomized into one ...19947853130
microbiota of rapidly progressive periodontitis lesions in association with clinical parameters.the microbial population in 73 rapidly progressive periodontitis (rpp) lesions in 10 young adults aged 25 to 35 years (5 males, 5 females) was studied in relation to the clinical parameters probing depth, bleeding on probing, and suppuration, which were recorded at the sampled sites. porphyromonas gingivalis was found to predominate (26.7%) in 73 periodontal lesions with clinical probing depth > 6 mm, followed by bacteroides forsythus (23.6%), and prevotella intermedia (15.7%). the prevalence of ...19947853132
peptostreptococcal infection in children.680 peptostreptococcus sp. (ps) were recovered from 598 (34%) of 1,750 specimens obtained from 554 patients. they included 103 ps asaccharolyticus, 74 ps magnus, 56 ps prevotii, 51 ps micros, 46 ps anaerobius, 11 ps morbilorum, and 10 ps saccharolyticus. most infections were polymicrobial (in 553 instances or 92%) but in 45 (8%), ps were recovered in pure culture. most ps were isolated from abscesses (237), ears (104), peritoneal fluid (95), lung infections (66), bone (30) and sinuses (24). pred ...19947855547
immunopathological activities of extracellular products of streptococcus mitis, particularly a superantigenic fraction.previously, we prepared extracellular products, fractions f-1 and f-2 of streptococcus mitis 108, an isolate from the tooth surface of an infant, and showed that f-1 exhibited inflammatory cytokine-inducing activities. in the present study, we present evidence that fraction f-2 induced human t-cell proliferation in the presence of irradiated human peripheral blood mononuclear cells and selectively activated t cells bearing v beta 2 and v beta 5.1 in the t-cell receptor. f-1, on the other hand, s ...19957868248
in-vitro activity of clinafloxacin (ci-960) and pd 131628-2 against anaerobic bacteria.the antimicrobial activities of two new quinolones, ci-960 and pd 131628-2 were determined against 339 strains of anaerobic bacteria and compared to cefoxitin, imipenem and metronidazole. the nccls-approved wadsworth agar dilution technique with brucella-lysed blood agar was used throughout the study. breakpoints of the new quinolones are 2 mg/l, and breakpoints for cefoxitin, imipenem and metronidazole are 32, 8 and 16 mg/l, respectively. ci-960 displayed excellent activity, inhibiting all stra ...19947868409
clostridial infection in children.a survey of the isolation of clostridium spp. from 1543 specimens sent to anaerobic microbiology laboratories revealed 113 isolates from 107 specimens (7.0% of all specimens) from 96 children. the isolates comprised 43 (38%) unidentified clostridium spp., 37 (33%) c. perfringens, 13 (12%) c. ramosum, five (4%) c. innocuum, six (5%) c. botulinum, three (3%) c. difficile, two (2%) c. butyricum, and one isolate each of c. bifermentans, c. clostridiiforme, c. limosum and c. paraputrificum. most clos ...19957869351
head and neck space infections in infants and children.a retrospective study was performed in 117 children with head and neck space infections treated at the children's hospital of pittsburgh from january 1986 through june 1992. peritonsillar space infections were the most common (49%), followed by retropharyngeal (22%), submandibular (14%), buccal (11%), parapharyngeal (2%), and canine (2%) space infections. the most common pathogens isolated (n = 78) were the aerobes beta-hemolytic streptococcus (18%) and staphylococcus aureus (18%), the anaerobes ...19957870436
the gingival immune response to periodontal pathogens in juvenile periodontitis.a gingival explant culture system was utilized to evaluate the reactivity of local immunoglobulins produced by juvenile periodontitis tissue. gingival explant culture supernatant fluids were screened, via a standardized dot-immunobinding assay, for antibodies reactive to: actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, eikenella corrodens, peptostreptococcus micros, peptostreptococcus anaerobius, capnocytophaga ochracea, eubacterium no ...19947870467
degradation of immunoglobulin g by periodontal bacteria.several subgingival microorganisms were tested for their ability to utilize human immunoglobulin g (igg) as a substrate for growth. this was done using a protein-free chemically defined medium, supplemented with igg. stimulation of growth was observed for capnocytophaga ochracea, porphyromonas asaccharolytica, porphyromonas endodontalis, porphyromonas gingivalis, prevotella intermedia, prevotella oralis, lactobacillus catenaforme and streptococcus intermedius. immunoelectrophoresis, sodium dodec ...19947870469
microbiologic tests in epidemiologic studies: are they reproducible?microbiologic assessments are often included in longitudinal studies to elucidate the significance of the association of certain gram-negative bacteria and the development of periodontal diseases. in such studies, the reliability of methods is crucial. there are several methods to identify putative pathogens, and some of them are commercially available. the purpose of the present study was to compare the reproducibility of four different methods for detecting actinobacillus actinomycetemcomitans ...19947871359
activity of wy-49605 compared with those of amoxicillin, amoxicillin-clavulanate, imipenem, ciprofloxacin, cefaclor, cefpodoxime, cefuroxime, clindamycin, and metronidazole against 384 anaerobic bacteria.the national committee for clinical laboratory standards agar dilution method was used to compare the in vitro activity of wy-49605 (also called sun/sy 5555 and alp-201), a new broad-spectrum oral penem, to those of amoxicillin, amoxicillin-clavulanate, imipenem, ciprofloxacin, cefaclor, cefpodoxime, cefuroxime, clindamycin, and metronidazole against 384 clinically isolated anaerobes. these anaerobic organisms included 90 strains from the bacteroides fragilis group, 87 prevotella and porphyromon ...19947872754
major methodology-dependent discordant susceptibility results for bacteroides fragilis group isolates but not other anaerobes.two standardized susceptibility test methods, a broth microdilution (bmd) and agar dilution (ad) method were performed on a total of 441 clinical isolates of anaerobes with ceftizoxime, cefotaxime, ceftriaxone, cefoxitin, piperacillin, and metronidazole. against the 339 strains of the bacteroides fragilis group bmd minimum inhibitory concentration (mic) values were lower than those from ad testing for all the beta-lactams. overall for the b. fragilis group and the beta-lactams, the mode mic valu ...19947874880
isolation of genes encoding beta-d-xylanase, beta-d-xylosidase and alpha-l-arabinofuranosidase activities from the rumen bacterium prevotella ruminicola b1(4).prevotella ruminicola b1(4) is a strictly anaerobic, gram-negative, polysaccharide-degrading rumen bacterium. xylanase activity in this strain was found to be inducible, the specific activity of cells grown on xylan being increased at least 20-fold by comparison with cells grown on glucose. ten bacteriophage clones expressing xylanase activity were isolated from a lambda embl3 genomic dna library of p. ruminicola b1(4). these clones were shown to represent four distinct chromosomal regions, base ...19957875560
the effect of carbohydrates on the expression of the prevotella ruminicola 1,4-beta-d-endoglucanase.the beta-1,4-endoglucanase of the ruminal bacterium, prevotella ruminicola b14, hydrolysed carboxymethylcellulose and barley glucan but not xylan or mannan. endoglucanase activity was present in 88- and 82-kda proteins, and there was at least a 20-fold variation in endoglucanase activity when p. ruminicola b14 was grown on different sugars. the highest activities were observed with mannose, cellobiose or xylose and little activity was observed with sucrose, arabinose or rhamnose, p. ruminicola b ...19957875579
[subgingival plaque due to gingivitis and inactive periodontitis sites in the adult periodontitis patient].there are several discrepancies with respect to the composition of subgingival plaque in gingivitis and in inactive adult periodontitis (ap). in this study we compared subgingival plaque samples taken from gingivitis sites to those from inactive periodontitis sites of the same patients. of 44 patients plaque samples from 86 gingivitis sites and 92 periodontitis sites were analysed. darkfield microscopy showed a higher proportion of spirochetes and mobile rods in the periodontitis sites. analysis ...19957878415
chemotaxonomy of bacteroides: a review.the loose definition of bacteroides, some species of which are important etiologic agents of oral diseases, has enabled isolates with only marginal similarities to be reposited in this genus. many attempts have been made over the years to improve the taxonomy of this heterogeneous group of bacteria. the present article reviews major chemotaxonomic characters and techniques that have been used for this purpose: pigmentation, metabolites, whole-cell fatty acids, phospholipids, isoprenoid quinones, ...19947887145
antimicrobial susceptibility of periodontopathic bacteria associated with failing implants.the aim of this study was to examine the subgingival microflora associated with failing implants, and to determine their susceptibility to commonly used antibiotics in periodontal therapy and dental practice. thirteen partially edentulous patients with 19 failing implants were selected. clinical examination included probing depth, attachment level, gingival index, plaque index, and radiographic analyses. two subgingival plaque samples were taken from each failing implant and analyzed for microbi ...19957891253
[production of monoclonal antibody that recognize the common antigen of black-pigmented bacteroides and application in pulp and periapical diseases]. 19937900976
adhesin degradation: a possible function for a prevotella loescheii protease?prevotella loescheii pk1295 produces at least 3 proteases that are separable by isoelectric focusing. one of these proteases, an enzyme with an isoelectric point at 8.5 and an m(r) of 36,000, hydrolyzes the fimbria-associated adhesin on p. loescheii responsible for coaggregation with streptococcus oralis 34, as well as gelatin, casein and fibrin. the action of this protease may contribute to the detachment of p. loescheii from its streptococcal coaggregation partner and provide a mechanism for b ...19937903444
antibacterial action of natural honey on anaerobic bacteroides.two samples of natural honey were tested for their antibacterial effect on bacteroides, mainly the pathogenic black pigmented b. melaninogenicus isolated from ten cases of dental infections (dental abscesses and chronic osteomyelitis). these organisms were subjected to the effect of natural and diluted honey (50%), in broth and solid cultures. the results were compared with those of the same organisms incubated with saturated glucose solution, which showed less inhibition, indicating that the in ...19937905406
characterization of prevotella intermedia and prevotella nigrescens isolates from periodontic and endodontic infections.the occurrence and surface properties of prevotella intermedia and p. nigrescens in healthy sites and in periodontic and endodontic infections were studied among 73 strains, tentatively identified as p. intermedia. fifteen strains were from necrotic root canal infections, 41 were from periodontal samples, and 17 isolates were obtained from healthy gingival sites. identification of isolates as either p. intermedia or p. nigrescens was based on differences in malate and glutamate dehydrogenase ele ...19947907659
genetic diversity and phylogenetic relationships among strains of prevotella (bacteroides) ruminicola from the rumen.a high degree of genetic diversity among 29 strains of prevotella (bacteroides) ruminicola from the rumen was revealed by comparing restriction fragment length polymorphisms in 16s rrna genes, sodium dodecyl sulfate-polyacrylamide gel profiles of total-cell proteins, and g + c contents of chromosomal dnas. in order to obtain information on phylogenetic relationships, the sequences of a 389-bp region of the 16s rrna gene, including variable regions 4 and 5, were compared for 10 strains. these 10 ...19947910475
role of structural and extracellular virulence factors in gram-negative anaerobic bacteria.gram-negative anaerobic bacteria belonging to the genera bacteroides, prevotella, and porphyromonas represent the most common cause of endogenous, usually mixed, infections occurring after abdominal or gynecologic surgery. anaerobes are important pathogens in oral-cavity infections as well as in systemic infections that originate from the mouth. clinical interest in these organisms is linked to the therapeutic problems usually encountered in treating mixed infections. despite their clinical rele ...19947916215
cloning and dna sequence analysis of the region containing attp of the temperate phage phi ar29 of prevotella ruminicola ar29.phage phi ar29 was shown to exist as a prophage integrated into the chromosome of prevotella ruminicola ar29. by dna hybridization studies, the point of integrative recombination on the phage genome (attp) was located on a 4.5 kb ecorv fragment. after preliminary mapping with restriction endonucleases, a 2.8 kb ecorv/hindiii fragment was isolated, cloned in escherichia coli and sequenced. dna hybridization localized the attp site to the vicinity of an internal drai site. sequence analysis showed ...19947921261
short-term effect of full-mouth extraction on periodontal pathogens colonizing the oral mucous membranes.in this study, we investigate the prevalence of selected periodontal pathogens on the oral mucous membranes before and after full-mouth tooth extractions in patients with severe periodontitis. 8 patients were microbiologically examined 2 x before and 2 x after extraction; several locations on the oral mucous membranes, saliva, supra- and subgingival plaque, were sampled. besides their presence in subgingival plaque, we detected before extraction on the mucous membranes actinobacillus actinomycet ...19947929861
presence of treponema denticola and porphyromonas gingivalis in children correlated with periodontal disease of their parents.considerable evidence exists suggesting that periodontal disease is due to the overgrowth of a finite number of specific bacteria such as porphyromonas gingivalis, actinobacillus actinomycetemcomitans, treponema denticola, bacteroides forsythus, and prevotella intermedia, among others. three of these organisms-p. gingivalis, t. denticola, and b. forsythus-can be easily detected in plaque samples by the hydrolysis of the synthetic trypsin substrate benzoyl-dl-arginine-naphthylamide (bana). the ai ...19947929977
characteristics of older adults experiencing periodontal attachment loss as gingival recession or probing depth.while attachment loss currently is the best field examination indicator that periodontal disease has progressed, this measure is not disaggregated enough to differentiate between attachment loss that results in deeper pockets and attachment loss that results primarily in recession of the gingiva. if these two conditions have different etiologies, then efforts at predicting who will experience the condition from a single, general risk assessment model will be difficult. the purpose of this paper ...19947932023
changes in root canal microbiota during the development of rat periapical lesions.periapical lesions are reproducibly induced in rats by pulp exposure and infection from the oral cavity. lesions expand rapidly between day 7 and day 15-20 (active phase), with slowed expansion thereafter. in the present study we characterized the root canal microbiota present during the active phase of lesion development in this system. the mandibular first molars of sprague-dawley rats were exposed on day 0. the teeth were extracted after 7 days (n = 10 animals) and 15 days (n = 10), and the m ...19947936717
the oral gram-negative anaerobic microflora in young children: longitudinal changes from edentulous to dentate mouth.eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. the composition of oral gram-negative anaerobic microflora was investigated in 21 young children (mean age 32 months) with primary dentition. the bacterial findings of samples were compared with those of the same children collected at their edentulous infant period (mean age 3 months). during the primary period, 2 samples were collected from each child: a sample with d ...19947936718
effect of lactoferrin on interaction of prevotella intermedia with plasma and subepithelial matrix proteins.a lactoferrin-binding protein with an estimated molecular mass of 57 kda was identified in the cell envelope of prevotella intermedia by gel electrophoresis and western-blot analysis. peroxidase-labeled bovine lactoferrin and human lactoferrin showed similar specific binding to this protein. whole cells of p. intermedia were also examined for interactions with 5 125i-labeled plasma and subepithelial matrix proteins. a high degree of binding was found with fibronectin, collagen type i and type iv ...19947936724
evidence for natural horizontal transfer of tetq between bacteria that normally colonize humans and bacteria that normally colonize livestock.though numerous studies have shown that gene transfer occurs between distantly related bacterial genera under laboratory conditions, the frequency and breadth of horizontal transfer events in nature remain unknown. previous evidence for natural intergeneric transfers came from studies of genes in human pathogens, bacteria that colonize the same host. we present evidence that natural transfer of a tetracycline resistance gene, tetq, has occurred between bacterial genera that normally colonize dif ...19947944364
in vitro activity of meropenem compared with imipenem, metronidazole, ampicillin, and ampicillin/sulbactam against anaerobes.the aim of the present study was to compare the in vitro activity of meropenem (ici 194660, cas 96036-03-2) with imipenem, metronidazole, clindamycin, ampicillin and ampicillin/sulbactam against a variety of anaerobic bacteria using an agar dilution method. 423 clinical isolates were tested belonging to 70 species of 15 anaerobic genera. they included bacteroides fragilis (n = 62), bacteroides thetaiotaomicron (n = 45), prevotella bivia (n = 11), fusobacterium nucleatum (n = 12), clostridium per ...19947945523
characterization of a new type of bacteroides conjugative transposon, tcr emr 7853.results of previous investigations suggested that the conjugative transposons found in human colonic bacteroides species were all members of a closely related family of elements, exemplified by tcr emr dot. we have now found a new type of conjugative transposon, tcr emr 7853, that does not belong to this family. tcr emr 7853 has approximately the same size as the tcr emr dot-type elements (70 to 80 kbp) and also carries genes encoding resistance to tetracycline (tcr) and erythromycin (emr); howe ...19947961412
clinical and microbiological status of osseointegrated implants.peri-implantitis, an inflammatory response around implants, has a poorly defined etiology and pathogenesis. to better understand the role of specific microorganisms in this disease process, clinical and microbiological parameters were examined in 24 patients with 98 osseointegrated implants. sites were evaluated for probing depth (pd), plaque/calculus index (pi), gingival bleeding index (gbi), mobility, and crevicular fluid flow rate (cffr). actinobacillus actinomycetemcomitans, porphyromonas gi ...19947965553
effects of crude leaf extracts of acalypha torta against some anaerobic bacteria. 19947967651
increased activity of a new chlorofluoroquinolone, bay y 3118, compared with activities of ciprofloxacin, sparfloxacin, and other antimicrobial agents against anaerobic bacteria.a total of 435 clinical isolates of anaerobes were tested with a broth microdilution method to determine the activity of bay y 3118 compared with those of other agents against anaerobic bacteria. all strains of bacteroides capillosus, prevotella spp., porphyromonas spp., fusobacterium spp., clostridium spp., eubacterium spp., peptostreptococcus spp., and veillonella parvula were susceptible (mics of < or = 2 micrograms/ml) to bay y 3118. against the 315 strains of the bacteroides fragilis group, ...19947979307
descriptions of prevotella tannerae sp. nov. and prevotella enoeca sp. nov. from the human gingival crevice and emendation of the description of prevotella zoogleoformans.prevotella tannerae sp. nov. and prevotella enoeca sp. nov. from the human gingival crevice are described. these organisms are obligately anaerobic, non-spore-forming, nonmotile, gram-negative, rod-shaped bacteria that ferment carbohydrates and produce succinic and acetic acids. bile inhibits growth. some strains (38%) of p. tannerae produce colonies with a tan to black pigment when they are grown on rabbit blood agar. the type strains are p. tannerae atcc 51259 and p. enoeca atcc 51261. in addi ...19947981091
a study of the subgingival microflora of hiv-seropositive and cd4 cell depleted children with hemophilia.the cultivable subgingival bacterial flora from three hiv-seropositive and cd4 cell depleted children with hemophilia were examined in this study. the numbers of cd4 cells of the subjected children ranged from 4.9 to 16.3 per mm3 blood. streptococcus species, including, beta-hemolytic streptococcus identified as streptococcus mutans and streptococcus sanguis, were predominant in the subgingival plaque samples. actinomyces species were also frequently found. gram-negative rods other than capnocyt ...19947987969
experimental gingivitis during pregnancy and post-partum: clinical, endocrinological, and microbiological aspects.the purpose of this study was to assess whether an intensive oral hygiene regimen practised during pregnancy results in a clinically healthy gingival state, and to assess whether experimentally-induced gingivitis differs in severity during pregnancy as compared to post-partum. in addition, levels of black-pigmented gram negative anaerobes at subgingival and oral mucosal sites and plasma concentrations of free estrogens and prosterone were determined. these parameters were studied during a 14-day ...19947989619
locally delivered minocycline and guided tissue regeneration to treat post-juvenile periodontitis. a case report.a case of post-juvenile periodontitis in a 28-year-old female patient is described along with new periodontal treatment modalities. administration of minocycline-hcl with local drug delivery system was introduced as a part of initial periodontal therapy following microbiological and immunological examinations. the lesions were subsequently treated by guided tissue regeneration, which resulted in considerable gain of attachment with minimal recession of marginal gingiva. this observation suggests ...19947990019
the influence of supragingival plaque control on clinical and microbial outcomes following the use of antibiotics for the treatment of periodontitis.although supragingival plaque control is essential to successful periodontal therapy, the role of plaque control following systemic antibiotic use in periodontal disease has not been well defined. this study evaluated, following antibiotic use, which clinical and microbial parameters appeared to be influenced primarily by the antibiotics, independent of plaque control, and which outcomes appeared to be dependent on plaque control. two hundred thirty-six patients (236) with moderate to severe per ...19947990021
[the use of solid-phase immunoenzyme analysis for the diagnosis of a suppurative infection of the maxillofacial area caused by bacteroides melaninogenicus and staphylococcus aureus].in 186 patients with odontogenic infection the level of antibodies to b.melaninogenicus polysaccharide antigen, teichoic acid and s.aureus polysaccharide was determined in the enzyme-linked immunosorbent assay (elisa). the positive values of elisa indices were found to coincide with the data of bacteriological study in more than 90% of cases. the role of b.melaninogenicus and s.aureus associations in the development of purulent infections was confirmed with the use of elisa in 34.2% of the patie ...19947992536
[the development of a test system for determining antibodies to bacteroides melaninogenicus using solid-phase immunoenzyme analysis].a test system for enzyme immunoassay of antibodies to bacteroides melaninogenicus has been developed. the authors describe a method for the preparation of the protein and polysaccharide antigens with the use of ultrasonic, lysozyme, and beta-naphthol treatment. a total of 105 blood serum samples, 46 samples of gingival blood and gingival fluid from patients with maxillofacial phlegmons and periodontitis were examined, making use of the 1 +/- 3 omega index (from the values in healthy donors). the ...19937994533
alteration of biological properties of bacterial lipopolysaccharide by calcium hydroxide treatment.bacterial lipopolysaccharide (lps) plays a major role in the development of periapical bone resorption. although the chemical properties of lps are altered by treatment with an alkali such as calcium hydroxide, the effects of calcium hydroxide on the biological properties of lps are not known. the purpose of this study was to investigate whether treatment of lps with calcium hydroxide alters its biological action as measured by human monocyte secretion of prostaglandin e2. monocyte cell cultures ...19947996084
otitis media: microbiology and management.otitis media (om) is a common childhood disease and one that can cause significant morbidity. a knowledge of the pathogens responsible for om can assist in the selection of the most appropriate treatment regimen and can minimize complications that may require surgery. the microbiology of acute, serous, and chronic om is reviewed. the major organisms recovered from about three quarters of acute om and half of serous om cultures are haemophilus influenzae, streptococcus pneumoniae, and moraxella c ...19947996627
[bacteroides melaninogenicus, during fournier's gangrene].in two patients (l.c., case no. 5834/215/90 and i.j., case no. 18760/140/91) fournier's gangrene was diagnosed. from samples of necrotic and stinking secretion, strains of bacteroides were isolated in anaerobic conditions. application of metronidazole for treatment and surgical cleaning of the wound resulted in healing of one patient, while the second died.19947996937
degradation and utilization of forage hemicellulose by rumen bacteria, singly in coculture or added sequentially.procedures for sequential addition experiments were developed to study the mechanisms involved in the synergistic and inhibitory interactions observed in forage hemicellulose digestion by rumen bacterial cocultures. one organism was allowed to ferment a forage substrate, the culture tube was sterilized and then inoculated with a second organism. no differences were found in the extent of degradation or utilization between fermentations sterilized by oxidation or heat, and based on ease of handli ...19948002478
the role of neuraminidase in haemagglutination and adherence to colon widr cells by bacteroides fragilis.the role of neuraminidase in haemagglutination and adherence to colon widr cells by eight strains of bacteroides fragilis and four strains of oral black-pigmented gram-negative anaerobes was studied. neuraminidase treatment resulted in a very small increase of haemagglutination by some of the strains but had no effect on adherence to widr cells by all bacterial strains tested except one strain of prevotella intermedia (hg 110). inhibition of neuraminidase had no effect on haemagglutination or ad ...19948006931
macaca fascicularis as a model in which to assess the safety and efficacy of a vaccine for periodontitis.we have assessed macaca fascicularis as a potential model in which to test the efficacy and safety of a vaccine for periodontitis. twenty-eight animals were surveyed and 20 studied in more detail. clinical periodontal status was assessed, the subgingival microflora analyzed especially for the presence and proportions of porphyromonas gingivalis and titers and avidities of serum antibodies reactive with p. gingivalis measured. probing depths ranged from 0.90 mm to 3.80 mm, gingival index scores f ...19948008428
the most frequently isolated gram-negative anaerobes in saliva and subgingival samples taken from young women.stimulated saliva and a pooled subgingival sample from the first molars were collected from 30 caucasian women (mean age 30 years) for the presence of gram-negative anaerobic bacteria. the samples were inoculated on nonselective and selective media. the gram-negative anaerobic species occurring in the oral cavity of more than 50% of the subjects were selected for further analysis. fusobacterium nucleatum and prevotella melaninogenica were not recovered from subgingival sites without a simultaneo ...19948008430
hydrolytic enzymes and lectin-binding activity of black-pigmented anaerobic rods.recent taxonomic studies on black-pigmented anaerobic rods, a group of bacteria found on mucosal surfaces of humans and animals, led to the subdivision of existing species and to the creation of new species. the aim of this study was to characterize all 11 currently recognized species of black-pigmented bacteria (55 strains) for their ability to hydrolyse a variety of natural and synthetic substrates and for their lectin reactivity. although most of the strains demonstrated some activity against ...19948012604
prevotella bivia as an unusual cause of endocarditis.a case of monomicrobial endocarditis due to prevotella bivia in a 60-year-old man without previous cardiac lesions is reported. the extremely indolent course with multiple systemic emboli as the only clinical manifestation occurring at least seven months before diagnosis and the persistently negative blood cultures were remarkable features of this case. the incidence, clinical characteristics, treatment and outcome of published cases of infective endocarditis due to anaerobic bacteria are briefl ...19948013486
comparison of the e-test and conventional agar dilution methods for susceptibility testing of gram-negative anaerobic rods.the e test was used to determine the susceptibility of 209 anaerobic gram-negative rods to ampicillin, amoxicillin-clavulanate, ticarcillin, ticarcillin-clavulanate, piperacillin, piperacillin-tazobactam, clindamycin, chloramphenicol, and cefoxitin. minimum inhibitory (mics) were read where growth intersected the strips or where the upper indentation intersected the strips for beta-lactamase inhibitor-containing strips. reference mics were performed by the national committee for clinical laborat ...19948026154
in vitro activity of biapenem (l-627), a new carbapenem, against anaerobes.we tested 441 clinical strains of anaerobes by using a broth microdilution method to determine the in vitro activity of biapenem for comparison with those of other agents. biapenem had activity comparable to those of imipenem and meropenem against all groups of anaerobes with mics for 90% of the strains tested of 0.06 to 2 micrograms/ml. against the bacteroides fragilis group, biapenem was more active than ampicillin-sulbactam, ticarcillin-clavulanate, piperacillin, cefoxitin, cefotaxime, and ce ...19948031067
influence of yucca shidigera extract on ruminal ammonia concentrations and ruminal microorganisms.an extract of the desert plant yucca shidigera was assessed for its possible benefit in ruminal fermentation. the extract bound ammonia in aqueous solution when concentrations of ammonia were low (up to 0.4 mm) and when the extract was added at a high concentration to the sample (20%, vol/vol). the apparent ammonia-binding capability was retained after autoclaving and was decreased slightly following dialysis. acid-precipitated extract was inactive. no evidence of substantial ammonia binding was ...19948031077
fusobacterial infections in children.a total of 243 strains of fusobacteria species was recovered from 226 of 1399 (16%) specimens obtained from 213 children. the strains included 65 (27%) fusobacterium sp., 144 (59%) fusobacterium nucleatum, 25 (10%) fusobacterium necrophorum, five (2%) fusobacterium varium, three (1%) fusobacterium mortiferum, and one (0.4%) fusobacterium gonidiaformans. most fusobacteria species were recovered from patients with abscesses (100), aspiration pneumonia (24), paronychia (15), bites (14), chronic sin ...19948034995
bacteria invading periapical cementum.the aim of this study was to determine whether microorganisms invade periapical cementum of human teeth from the adjacent periapical lesions. we therefore attempted to isolate microorganisms from periapical cementum through the adoption of standard anaerobic procedures for obligate anaerobes. samples of cementum were taken from 10 amputated tooth roots at the time of apicoectomy. from two of these samples, bacteria were recovered after anaerobic incubation, but no bacteria were recovered after a ...19948035155
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