| some contributions of electron microscopy to the study of the rickettsiae. | electron microscopy has provided valuable insights into the study of rickettsiae as intracellular parasites from several important perspectives. this tool has allowed researchers to delineate the fine structural features of these organisms and to show that they truly resemble free-living bacteria. furthermore, it has been shown that there are subtle, but distinct differences in the outer envelope structure of some members of the genus rickettsia that may explain reported differences in tinctoria ... | 1991 | 1909242 |
| [prevalence of antibodies against coxiella burnetii in a healthy population in lanzarote (canary islands)]. | the canary islands area now appears to be a q-fever endemic zone, especially the west side (la palma island). the situation in the eastern islands in unknown. in order to evaluate the seric prevalence of coxiella burnetii, 100 serum samples that were taken from the adult population of lanzarote and, following strict criteria, were analysed using a complement fixation test; blood donors and patients who had suffered a recent infection were excluded. the study was carried out during november/1986. ... | 1991 | 1912190 |
| [long-term fever in a patient born in india]. | | 1991 | 1925230 |
| a plasmid-encoded surface protein found in chronic-disease isolates of coxiella burnetti. | the cbbe' gene codes for the e' protein of coxiella burnetii and was detected in genomic dna from all known human isolates of the biotzere strain but not in dna from the other five strains of c. burnetti. the biotzere strain is strictly associated with chronic disease in humans. extrinsic iodination of biotzere strain cells radiolabeled a 55-kda protein which comigrated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis with the e' protein synthesized in vitro from recombinants contain ... | 1991 | 1937836 |
| characterization of naturally acquired antibody responses to a recombinant fragment from the n-terminus of plasmodium falciparum glycoprotein 195. | antibody responses to the glycoprotein precursor of the major merozoite surface antigens of plasmodium falciparum (gp195) were investigated in acutely infected thai adults. specific igg antibody was assayed by enzyme-linked immunosorbent assay using a recombinant fragment derived from the n-terminal region of gp195 as the capture antigen. two control groups were found to be without significant cross-reacting antibody. among occupationally exposed soldiers, 84 of 85 men developed positive antibod ... | 1991 | 1951865 |
| atypical pneumonia. | the atypical pneumonia syndrome usually implies a benign illness where systemic complaints predominate over respiratory symptoms. cough is prominent; chest radiographic findings are varied. many organisms are associated with this syndrome. mycoplasma pneumoniae, chlamydia psittaci, chlamydia pneumoniae, coxiella burnetii, and francisella tularensis are reviewed in this article. | 1991 | 1955701 |
| respiratory infections transmitted from animals. | the physician's approach to the differential diagnosis of obscure, atypical pneumonias has changed. the physician needs to expand the diagnostic search when confronted with a perplexing, progressive atypical pneumonia. diseases examined in this article include anthrax, psittacosis, q fever, tularemia, pasteurellosis, melioidosis, rocky mountain spotted fever, and the plague. | 1991 | 1955704 |
| erythromycin and the treatment of coxiella burnetii pneumonia. | erythromycin is commonly used for the empirical treatment of community-acquired pneumonia, but there is some concern about its usefulness when q fever is suspected because of in-vitro studies showing a lack of efficacy against coxiella burnetii. this study was undertaken to assess the clinical value of the antibiotic in this setting. nineteen patients with q fever pneumonia treated with a variety of antibiotics considered ineffective against c. burnetii, were reviewed. eleven patients who receiv ... | 1991 | 1960125 |
| coxiella burnetii fails to stimulate human neutrophil superoxide anion production. | this study investigated the release of superoxide anion (o2-) as an indicator of the oxidative metabolism of human neutrophils during the phagocytosis of phase i coxiella burnetti. human neutrophils were incubated for 1 hr at 37 degrees c with opsonized or unopsonized viable phase i coxiella burnetii (moi was 100 : 1) and superoxide anion formation was measured by the reduction of ferricytochrome c. the data revealed that during its phagocytosis by human neutrophils, c. burnetii (opsonized or un ... | 1990 | 1975727 |
| dynorphin increases in the dorsal spinal cord in rats with a painful peripheral neuropathy. | it is known that painful tissue injury evokes an increase in dynorphin in spinal neurons. it is not known, however, whether dynorphinergic systems respond similarly to the pain that accompanies peripheral neuropathy. radioimmunoassays and immunocytochemistry were used to evaluate changes in dynorphin a(1-8) in the spinal cord of rats with a painful peripheral neuropathy. the neuropathy is the result of a constriction injury that is created by tying loose ligatures around the common sciatic nerve ... | 1990 | 1978300 |
| molecular biology of the cuticle collagen gene families of caenorhabditis elegans and haemonchus contortus. | | 1990 | 1978527 |
| indirect haemolysis test in q-fever. | sheep erythrocytes sensibilized with the lipopolysaccharide (lps) extracted from coxiella burnetii phase 1 cells were used in indirect haemolysis test (iht). the test was highly sensitive and specific. its use seemed reasonable when following the chronic course of q-fever by serologic methods. | 1990 | 1981447 |
| thoracocardiography. part 1: noninvasive measurement of changes in stroke volume comparisons to thermodilution. | the thoracocardiograph (tcg) is a new noninvasive monitoring device that measures cardiac oscillations transmitted to the external surface of the thorax. it consists of 2.5 cm in height, elastic inductive plethysmographic transducers placed transversely in the proximity of the xiphoid process to provide changes in cross-sectional area on a transverse plane across the minor ventricular axis. cardiac oscillations synchronous with each heart beat are extracted from the respiratory signal during bre ... | 1991 | 1995217 |
| pathology and diagnosis of coxiella burnetii infection in a goat herd. | | 1991 | 2017831 |
| serologic survey for bovine pathogens in free-ranging european bison from poland. | from 1980 to 1983, blood was taken from 60 selected european bison (bison bonasus) in poland. serum samples were tested for the presence of antibodies against brucella abortus, 14 serovars of leptospira interrogans, chlamydia spp., coxiella burnetti; foot and and mouth disease virus, bovine leukemia virus and bovine herpes virus-1. in addition, an attempt was made to isolate bovine herpes virus-1 from the prepuce of selected bulls. serological tests suggested chlamydial infection in 28 bison, su ... | 1991 | 2023316 |
| comparison of different antibiotic regimens for therapy of 32 cases of q fever endocarditis. | we studied 32 cases of q fever endocarditis diagnosed in france between january 1985 and december 1989 to evaluate the efficacies of the different regimens of antibiotics used for treatment. each patient was monitored during the treatment (range, 12 to 60 months), and clinical and biological information was computerized. various treatments were prescribed, including doxycycline alone (9 cases) or in association with rifampin (4 cases), quinolones (16 cases), or sulfamethoxazole-trimethoprim (1 c ... | 1991 | 2039204 |
| efficacy and safety of recombinant human erythropoietin to prevent the anaemias of prematurity. european randomized multicenter trial. | | 1991 | 2040194 |
| q fever meningoencephalitis in five patients. | within the last four years, we have observed five patients with epidemiological, clinical, and serological features that were consistent with q fever meningoencephalitis. attempts to isolate coxiella burnetii from the cerebrospinal fluid of two patients were unsuccessful. neurological features ranged from coma, general seizures, confusion, to palsy and meningitis. all patients were febrile. these patients were neuroradiologicaly investigated. since 1984, four other cases have been reported in th ... | 1991 | 2044709 |
| [febrile state, headache and arthralgia]. | a 38-year-old man was admitted to the hospital with complaints of persistent fever up to 40 degrees c, arthralgias, headache and a nonproductive cough. the white-cell count was within the normal range but was markedly shifted to the left and demonstrated toxic granulations. sonographic examination of the abdomen revealed a slight enlargement of the spleen. a nonspecific reactive hepatitis which was clinically asymptomatic was detected by laboratory evaluation. diagnosis of an acute q fever was m ... | 1991 | 2052831 |
| [optimal combined use of rifampicin and immunomodulator of microbial origin in experimental q-rickettsia infection]. | multifactorial analysis of the combined use of rifampicin and an immunomodulator of the microbial origin, such as peptidoglycan, was performed on a model of experimental q fever in albino mice. on the basis of the experimental results, statistic polynomial models describing the weight of the murine spleens and the titers of the complement-binding antibodies were designed. it was shown that the action of the immunomodulator and antibiotic was highly synergistic with respect to the chemotherapeuti ... | 1991 | 2053816 |
| [chemotherapeutic effectiveness of doxycycline in experimental q fever]. | comparative chemotherapeutic efficacy of doxycycline administered orally or parenterally was studied on a model of experimental q fever in guinea pigs and albino mice. the advantage of the parenteral use of the antibiotic was observed only in the treatment of the albino mice. optimization of the doses and time of doxycycline use providing the maximum therapeutic effect and the required levels of the complement binding antibodies was achieved with employing the multifactorial analysis. | 1991 | 2053817 |
| an outbreak of cat-associated q fever in the united states. | q fever is usually acquired by contact with aerosols generated during parturition of domestic ungulates (e.g., sheep, cows, goats). in the maritime provinces of canada, parturient cats have also been implicated in its transmission. a 66-year-old woman from eastern maine developed high fever, rigors, headache, myalgias, pulmonary infiltrates, and elevated hepatocellular enzymes, and the diagnosis of acute q fever was confirmed serologically. she and 14 other family members had attended a family r ... | 1991 | 2056206 |
| analysis of the cells involved in the lymphoproliferative response to coxiella burnetii antigens. | vaccination with an inactivated, whole cell, q fever vaccine (q-vax) induces lasting antibody conversion and a positive delayed-type hypersensitivity (dth) skin reaction in about 60% of recipients but a long-lasting positive lymphoproliferative or mitogenic response to c. burnetii antigens with peripheral blood mononuclear cells (pbmc) in 85-95% of subjects. analysis of the lymphoproliferative response to c. burnetii antigens has now been made by fractionation-reconstitution experiments with pbm ... | 1991 | 2070564 |
| [prospects of using controlled-action lactoglobulins in the treatment of acute intestinal infections and dysbacteriosis in infants]. | the author summarizes the results of the preclinical study and clinical trial of the new biological drug lactoglobulin having an +anti-coli-proteus action. shows that the drug holds promise in the treatment of infants with acute intestinal infections and dysbacteriosis as well as in the enrichment of lactic mixtures used for artificial feeding of premature children and babies referred to the risk group. | 1990 | 2075046 |
| [recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation]. | every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ... | 1990 | 2075365 |
| [growth of coxiella burnetii in selected cell cultures]. | usefulness of monolayer cell cultures for c. burnetii multiplication was evaluated. continuous vero cell line and primary cell (cultures cell culture of monkey kidney cercopithecus aethiops, cell culture of chicken fibroblasts, diploid cell line of human embryo) was inoculated for this purpose. two coxiella burnetii strains suspensions were used as inoculum of standard henzerling strain and zamość strain isolated from an area of poland. primary cell lines were characterized by considerable degre ... | 1990 | 2087134 |
| [coxiella burnetii: serologic study in various populations]. | complement binding antibodies against coxiella burnetii were measured in 699 seric samples in order to ascertain the possible relationship between seric prevalence and the following factors: age, sex, living in rural or urban areas and socio-economical level. all patients were healthy: 180 were from a rural area 86 from guijo (cáceres); 36 from chiloeches (guadalajara) and 58 from bustarviejo (madrid); 519 were from an urban area (city of madrid). the samples from madrid city were classified by ... | 1990 | 2104096 |
| the changing pattern of antibiotic resistance of neisseria gonorrhoeae. | | 1990 | 2111274 |
| the changing pattern of antibiotic resistance of neisseria gonorrhoeae. | | 1990 | 2111274 |
| nucleotide sequence of htpb, the legionella pneumophila gene encoding the 58-kilodalton (kda) common antigen, formerly designated the 60-kda common antigen. | gene htpb, which encodes the 58-kilodalton protein of legionella pneumophila, was cloned in escherichia coli and its complete nucleotide sequence was determined. analysis of this sequence revealed an open reading frame of 1,644 nucleotides encoding a protein with a predicted molecular mass of 57,952 daltons. data obtained by amino-terminal sequencing of the purified 58-kilodalton protein agreed, except for one amino acid residue, with the predicted amino acid sequence, identifying this open read ... | 1990 | 2117582 |
| zoonotic infections in northern ireland farmers. | evidence of past zoonotic infection was investigated serologically in randomly selected northern ireland farmers. the percentage of farmers with antibody was: brucella abortus (0.7), leptospira interrogans serovars (8.1), borrelia burgdorferi (14.3), toxoplasma gondii (73.5), coxiella burnetii (28.0), chlamydia psittaci (11.1) and hantavirus (1.2). the results show that northern ireland farmers have been exposed in the past to zoonotic infections. it is not known if these infections contributed ... | 1990 | 2123457 |
| prevalence of antibodies to rickettsia conorii coxiella burnetii and rickettsia typhi in salamanca province (spain). serosurvey in the human population. | the salamanca province of spain is an endemic zone for mediterranean spotted fever. in this area, only one case of q fever has been reported and typhus group rickettsial diseases have never been diagnosed. to obtain a panoramic view of the presence of antibodies to the most ubiquitous rickettsial agents, 400 sera specimens from a statistically representative sample of the human population of salamanca city and its surrounding province were subjected to indirect microimmunofluorescent test for an ... | 1990 | 2123799 |
| [q fever. a familial outbreak of 5 cases]. | five cases of acute q fever diagnosed in the same family are reported. the epidemiological and clinical features, the therapy and evolution of the infections by coxiella burnetti are discussed. q fever is a condition to be considered in febrile illnesses or atypical pneumonia, particularly when developing in outbreaks. it is concluded that q fever should be considered as endemic in some areas of this country (basque country, catalonia...). finally, we think that antibiotic therapy (tetracyclines ... | 1990 | 2129681 |
| [necrotizing myositis caused by coxiella burnetii]. | | 1990 | 2152494 |
| reduction of drug accumulation and dna topoisomerase ii activity in acquired teniposide-resistant human cancer kb cell lines. | we have isolated stable teniposide (vm26)-resistant cell lines from human cancer kb cells by stepwise exposure to increasing doses of the drug. at each step, we have purified vm26-resistant cell lines. kb/vm-a, kb/vm-b, kb/vm-1, kb/vm-2, kb/vm-3, and kb/vm-4 showed 3-, 6-, 12-, 16-, 74-, and 95-fold higher resistance to vm26 than did kb. we have further characterized kb/vm-2 and kb/vm-4 which showed about 15- and 100-fold higher resistance to vm26 or etoposide (vp16) than did kb. both vm26-resis ... | 1990 | 2168282 |
| morphological aspects of inhibition of transformation of rat kidney cells infected with a temperature-sensitive mutant of the rous sarcoma virus by rat fibroblast interferon. | normal rat kidney cells (nrk) infected with a temperature-sensitive (ts) mutant of rous sarcoma virus (rsv) at the permissive temperature (33 degrees c) exhibit the morphological and growth characteristics of the transformed state. by the use of this system, it has been shown that the addition of rat fibroblast interferon to infected cells grown at a nonpermissive temperature (38 degrees c) prevents re-transformation when they are introduced to the permissive temperature. merely treating cells a ... | 1990 | 2168311 |
| in vitro cytotoxic effects of cobalt-containing dusts on mouse peritoneal and rat alveolar macrophages. | "hard metal disease" is a chronic lung disease characterized by the presence of interstitial fibrosis; the role of cobalt in the development of this entity is still debated. indeed, most cases have been observed in some workplaces (grinding) of the hard metal industry and in the diamond polishing industry whereas no similar cases have been reported from workers exposed to pure cobalt powders in cobalt refineries. this study was designed to assess the in vitro toxicity (ldh release, morphology) o ... | 1990 | 2168316 |
| impact of reduced cytochrome oxidase activity on peak oxygen consumption of muscle. | the impact of reduced muscle oxidative capacity on peak oxygen consumption and isometric performance was evaluated using an isolated rat hindlimb preparation perfused with a high oxygen delivery. capacity for electron transport was reduced with chloramphenicol (cap), an inhibitor of mitochondrial gene-coded protein synthesis. the activity of cytochrome oxidase, a mitochondrial cristae component, was reduced approximately 45% (p less than 0.005) in the mixed-fiber-type plantaris muscle. several f ... | 1990 | 2168365 |
| in vitro assembly of relaxosomes at the transfer origin of plasmid rp4. | during initiation of conjugative transfer of dna containing the transfer origin (orit) of the promiscuous plasmid rp4, the proteins trai, traj, and trah interact and assemble a specialized nucleoprotein complex (the relaxosome) at orit. the structure can be visualized on electron micrographs. site- and strand-specific nicking at the transfer origin in vitro is dependent on the proteins trai and traj and on mg2+ ions. substrate specificity is directed exclusively towards the cognate transfer orig ... | 1990 | 2168553 |
| effect of inhibition of na+/k(+)-adenosine triphosphatase on vascular action of vasopressin. | the present study was undertaken to examine the cellular interaction between a na+/k(+)-atpase inhibitor, ouabain, and arginine vasopressin (avp) in rat vascular smooth muscle cells (vsmc) in culture. preincubation with 10(-5) m ouabain for 60 min increased basal cytosolic free ca2+ [( ca2+]i) concentration and intracellular 45ca2+ uptake. ouabain, however, did not affect basal 45ca2+ efflux or avp-stimulated 45ca2+ efflux. as assessed by cell shape change, preincubation with 10(-5) m ouabain fo ... | 1990 | 2170449 |
| a randomized, controlled, double-blind, cross-over, clinical trial of q fever vaccine in selected queensland abattoirs. | a limited, randomized, blind, placebo-controlled trial of q fever and influenza vaccines has been conducted in three queensland abattoirs on a sequential analysis design. ninety-eight subjects were given q fever vaccine and 102 influenza vaccine. q fever cases were observed in unvaccinated workers in all three abattoirs during the period of observation. a total of seven q fever cases in one group, one more than the number required to achieve statistical significance between the two vaccine group ... | 1990 | 2182329 |
| [laboratory diagnosis of respiratory syncytial virus infection]. | we study 18 children with acute respiratory infection diagnosed early by direct immunofluorescence (dif) using monoclonal antibodies to synticial respiratory virus (srv). antibodies to adenovirus, influenza virus a and b, parainfluenza virus 1, 2 and 3, mycoplasma, q fever, herpes and cytomegalovirus (cmv) are also studied. we consider that in most cases the diagnosis of infections caused by srv cannot be established by complement fixation, and should be accompanied by some antigen detection tec ... | 1990 | 2189329 |
| the immune response in a cat-related outbreak of q fever as measured by the indirect immunofluorescence test and the enzyme-linked immunosorbent assay. | the isotypic immune response of 16 individuals who developed q fever pneumonia following exposure to an infected parturient cat was studied. the enzyme-linked immunosorbent (elisa) test was used to detect igm, iga, and igg antibodies to phase i and phase ii coxiella burnetii whole-cell antigens and to the phase i lipopolysaccharide. the indirect immunofluorescent antibody (ifa) test was also used to detect antibodies to phase i and phase ii whole cells. none of the 16 subjects developed antibodi ... | 1990 | 2192785 |
| induction of heat-shock proteins in coxiella burnetii. | | 1990 | 2198830 |
| host factors in the severity of q fever. | the author has reviewed different aspects of the role of immunocompetence in the development of q fever. coxiella burnetii lives within the phagolysosomes of infected cells. in animals, the immunosuppression caused by either cortisone or x-irradiation reactivates q fever. in humans, cases of q fever are reported in immunocompromised hosts suffering from leukemia, cancer, and human immunodeficiency virus infection (aids). similar data are reported with strict or facultative intracellular parasite ... | 1990 | 2198833 |
| characterization of the origin of dna replication of the coxiella burnetii chromosome. | | 1990 | 2198835 |
| similarity in pathogenic features in lung and peritoneal infection by coxiella burnetii, typhus group rickettsiae, and chlamydiae. | | 1990 | 2198836 |
| legionella pneumophila htpab heat shock operon: nucleotide sequence and expression of the 60-kilodalton antigen in l. pneumophila-infected hela cells. | a 60-kilodalton (kda) immunodominant antigen of legionella pneumophila is a heat shock protein (hsp) of the groel class of hsps. the gene (htpb) coding the 60-kda protein was localized to a 3.2-kilobase dna fragment of l. pneumophila cloned into puc19 (psh16) (p. s. hoffman, c. a. butler, and f. d. quinn, infect. immun. 57:1731-1739, 1989). the nucleotide sequence of the dna fragment cloned into m13 confirmed two open reading frames, htpa and htpb, that code for proteins of 96 and 548 amino acid ... | 1990 | 2205580 |
| importance of the portal venous pathway to the transport of intestinal triglyceride-rich lipoproteins in the preruminant calf. | | 1990 | 2206345 |
| prevalence of q fever infection in the addis ababa abattoir. | a total of 465 human sera were collected for a serological survey of q fever infection from the addis ababa, ethiopia, abattoir, in 1988. the subjects' sera were tested for antibodies against coxiella burnetti antigen using the complement fixation test. results indicate a prevalence rate of 6.5%. this finding clearly demonstrates serological evidence of post infection with q fever in employees of the abattoir. | 1990 | 2209579 |
| q fever in plymouth, 1972-88. a review with particular reference to neurological manifestations. | between 1972 and 1988 we have serologically confirmed 103 coxiella burnetii infections: 46 were acute, 5 were chronic, 52 represented past infections. details of 61 cases are presented. of acute cases 80% had respiratory involvement; at least 63% had pneumonias. the incidence (22%) of neurological complications was of particular interest; 40% of these patients had prolonged sequelae. one acutely ill patient died of fulminating hepatitis. patients with pre-existing pathology or immunosuppression ... | 1990 | 2209742 |
| philippine population development: a nursing concern. | in keeping with nurses' commitment to the preservation and care of human life, population development should be a major concern of the nursing profession in the philippines. the profession should ensure that the health system is oriented toward people's needs and formulate manpower strategies on the basis of these needs and population distribution. nurses are in an ideal position to advocate a family-centered approach to health care through their access to pregnant women and mothers. nurses c ... | 1990 | 2217534 |
| bactericidal effect of doxycycline associated with lysosomotropic agents on coxiella burnetii in p388d1 cells. | there is no consistently reliable treatment for endocarditis resulting from chronic coxiella burnetii infection, the causative agent of q fever. although certain antibiotics are recommended on the basis of their in vitro bactericidal activities, results of therapy with these antibiotics are often disappointing. to evaluate whether the currently recommended antibiotic susceptibility tests for c. burnetii give misleading results because of continued division of uninfected cells, thereby resulting ... | 1990 | 2221859 |
| occurrence of coxiella burnetii, rickettsia slovaca, and organisms resembling bacillary rickettsiae in their natural foci in slovakia 20 years after their first detection. | | 1990 | 2227718 |
| abelson murine leukemia virus transforms preneoplastic emu-myc transgene-carrying cells of the b-lymphocyte lineage into plasmablastic tumors. | e mu-myc transgenic mice were back-crossed to balb/c mice up to back-cross generation 3. the offspring that included transgene-carrying and -negative mice in approximately equal proportions were randomly divided into 2 groups. thirty-four mice (group i) were treated with pristane, followed by a-mulv, and 40 (group ii) were injected with a-mulv alone. altogether, 16 lymphoid tumors developed in group i and 17 in group ii. nine of the tumors in group i and 4 in group ii appeared as ascitic tumors. ... | 1990 | 2228313 |
| [meningoencephalitis caused by coxiella burnetii]. | meningoencephalitis caused by coxiella burnetii is exceptional and its clinical presentation is varied. we report a case which presented as transient central neurological deficits and intracranial hypertension without fever. the condition was diagnosed by indirect immunofluorescence. | 1990 | 2237097 |
| acute respiratory disease in spain: seven years of experience. | the clinical and epidemiologic features of viral and nonviral pathogens involved in acute respiratory diseases are described in the context of cases of infection (especially atypical pneumonia and bronchiolitis) studied at the centro nacional de microbiología, virología e immunología sanitarias in madrid during a 7-year period (1979-1986). these etiologies were demonstrated in 1,637 (36.2%) of 4,521 cases. among viruses, respiratory syncytial virus most frequently infected children; influenza vi ... | 1990 | 2237112 |
| [effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog]. | a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ... | 1990 | 2238764 |
| nucleotide sequence of coxiella burnetii superoxide dismutase. | | 1990 | 2243797 |
| [hibernal respiratory disorders]. | a long term serological surveillance of the acute respiratory illnesses was conducted, looking for infections by adenoviruses, influenza a.b.c. parainfluenza 1,2,3 and respiratory syncytial viruses as well as mycoplasma pneumoniae, coxiella burneti and chlamydia psittaci. the analysis of the results accumulated for the past 20 years was carried out. influenza c. coxiella burneti and chlamydia psittaci infections were rare and could not account for any epidemic prevalence. the other agents produc ... | 1990 | 2249087 |
| isolation of 16 strains of coxiella burnetii from patients by using a sensitive centrifugation cell culture system and establishment of the strains in hel cells. | q fever, caused by coxiella burnetii, may be acute or chronic. only a few strains of c. burnetii have been isolated due to the difficulty and hazard of isolation. we report here the isolation using a centrifugation shell vial technique of 16 new strains from patients suffering chronic q fever. twenty-four samples were inoculated onto human embryonic lung (hel) fibroblast cell monolayers growing in shell vials. c. burnetii was detected 6 days later by using immunofluorescence. samples from valves ... | 1990 | 2254424 |
| induction of carcinoembryonic-antigen-gene expression in human colorectal carcinoma by sodium butyrate. | the effect of sodium butyrate on the expression of the carcinoembryonic-antigen (cea) gene was studied in two poorly differentiated colorectal-carcinoma cell lines (clone-a and mip-101) and in one well-differentiated cell line (ls-174t); a.t.c.c. no. ccl 188). northern-blot and dot-blot analyses indicated a steady increase in cea mrna from day 4 to a maximal level by day 14 after these cells were exposed to 2 mm-sodium butyrate. studies using nuclear run-off assays followed by dot-blot hybridiza ... | 1990 | 2268282 |
| placental transfer and tissue distribution of vitamin e in pregnant rabbits. | alpha-tocopherol (vitamin e) is widely prescribed in neonatal intensive care units, in large doses and by different schedules, for the prevention of retrolental fibroplasia, intraventricular haemorrhage, bronchopulmonary dysplasia, and haemolytic anaemia. since the efficacy of the drug in premature newborns seems related to early administration, the physicochemical characteristics of the drug itself and available formulations limit the major therapeutic aim of promptly raising levels of vitamin ... | 1990 | 2271744 |
| immunization of cattle (bos indicus x bos taurus) against hyalomma anatolicum anatolicum using antigens derived from tick salivary gland extracts. | studies were conducted on 41 cross-bred (bos taurus x bos indicus) calves which were less than 1 year old. three types of salivary gland antigens (sg ag) viz. sg ag-i (whole), sg ag-ii (supernatant) and sg ag-iii (sediment), prepared from the ixodid tick, hyalomma anatolicum anatolicum, were used for immunizing these calves. the calves were divided into five groups. the first three groups were immunized with sg ag-i, -ii and -iii with freund's complete adjuvant (fca), respectively. the fourth gr ... | 1990 | 2276870 |
| clinical q fever in northern ireland 1962-1989. | q fever was diagnosed in 443 patients in northern ireland between 1962 and 1989. from 1986 onwards there was an increase, which peaked in 1989 with 107 cases of whom 47 were infected in ballycastle, co antrim. there were three outbreaks and 21 clusters of patients with q fever. most cases were in april and may which correlated with the peak lambing and calving season. q fever mainly affected males in the 40-49 year old age group. county antrim had the highest prevalence rate of 40/100,000 popula ... | 1990 | 2278109 |
| [frequency of abortion and seroprevalence of the principal diseases causing ovine infectious abortion in the area of rabat (morocco)]. | a survey was carried out on 23 sheep flocks to estimate the frequency of abortion as well as the prevalence of antibodies against abortive infections. during the visit of each farm, a questionnaire was completed with the collaboration of the owner and blood samples were collected from all aborted ewes and some of those with normal lambing. a rate of 7% abortion was reached in both aborted and normally lambed ewes. anti-chlamydia psittaci antibodies were the most frequently detected (14 flocks). ... | 1990 | 2288452 |
| a prospective study of viral and mycoplasma infections in chronic inflammatory bowel disease. | seventy-two children with chronic inflammatory bowel disease were investigated for infections with various viruses and mycoplasma pneumoniae, chlamydia psittaci, and coxiella burnetii to determine whether these pathogens are associated with acute onset exacerbations. altogether 54 infections were identified serologically, of which 23 (42.6%) were associated with exacerbations. this corresponded to 24.2% of the recorded exacerbations during the study period. the respiratory pathogens accounted fo ... | 1990 | 2298361 |
| [lung infections with atypical features]. | the clinical difference between a lobar pneumonia caused by pneumococci or other bacterial agents and lower respiratory tract infections presenting with atypical symptoms is important. mycoplasma pneumoniae, chlamydia species, coxiella burnetii and several viruses amongst others are micro-organisms which cause atypical symptoms. all the time new types of micro-organisms like ureaplasma urealyticum are found as a cause of pneumonia with atypical symptoms. special diagnostic tools like cultures, s ... | 1990 | 2315905 |
| q fever encephalitis. | encephalitis is a rare but documented complication of acute q fever. we report here the case of a 48-year-old lady who presented with an acute illness characterised by influenza-like symptoms, pneumonia and neurological disturbance but in whom the serology was suggestive of chronic rather than acute q fever. | 1990 | 2319148 |
| vaccine prophylaxis of abattoir-associated q fever: eight years' experience in australian abattoirs. | during the period 1981-8 a clinical trial of a q fever vaccine (q-vax; commonwealth serum laboratories, melbourne) has been conducted in abattoir workers and other at-risk groups in south australia. volunteers in four abattoirs and visitors to the abattoirs were given one subcutaneous dose of 30 micrograms of a formalin-inactivated, highly-purified coxiella burnetii cells, henzerling strain, phase 1 antigenic state, in a volume of 0.5 ml. during the period, over 4000 subjects have been vaccinate ... | 1990 | 2323360 |
| nucleotide sequence of porcine insulin-like growth factor. 1:5' untranslated region, exons 1 and 2 and mrna. | | 1990 | 2326169 |
| [endocarditis caused by coxiella burnetii in aortic prosthesis. presentation of a case with a fatal course]. | a case of endocarditis caused by coxiella burnetii in a patient with an aortic starr-edwards prosthesis is described. the diagnosis was made by detecting high titres of antibody against coxiella burnetii phase i antigens. after 15 weeks of chemotherapy with tetracycline and cotrimoxazole, aortic prosthetic valve replacement was carried out because of haemodynamic deterioration and he died 4 days later. the histologic picture of the excised valve was consistent with endocarditis. coxiella burneti ... | 1990 | 2326533 |
| an epidemiological study of an outbreak of q fever in a secondary school. | five cases of clinical q fever were identified amongst students and staff of a somerset secondary school between 23 october 1987 and 21 december 1987. five goats which were kept at the school were found to have antibodies to coxiella burnetii phase ii. a cross-sectional study was conducted at the school in july 1988. a single cf test was used to identify evidence of prior infection, and a self-administered questionnaire was used to collect data on exposure variables and illness during 1987. four ... | 1990 | 2347385 |
| mixed infection of rickettsiella phytoseiuli and coxiella burnetii in dermacentor reticulatus female ticks: electron microscope study. | mixed infection of rickettsiella phytoseiuli and coxiella burnetii was investigated in hemolymph and organs of experimentally infected females of dermacentor reticulatus ticks. following intracoelomic infection, both agents, with the exception of gene's organ, multiplied well in the cells of the tick host's organs. two out of six developmental stages of r. phytoseiuli, i.e., crystal-forming and small dark particles, in dual infection with c. burnetii revealed marked morphological alterations. c. ... | 1990 | 2351845 |
| isolated coxiella burnetii synthesizes dna during acid activation in the absence of host cells. | two populations of coxiella burnetii were isolated from fibroblast tissue cultures and examined for their ability to synthesize dna when incubated in a defined medium. both the populations released by mechanical lysis of heavily infected host cells, as well as those recovered from the tissue culture medium, incorporated h3 32po4 into dna. incorporation occurred at ph 4.5 but not at ph 7.0, and proceeded for 12-15 h. when incorporation of [3h]thymidine was studied, only the organisms obtained by ... | 1990 | 2351956 |
| quantitative evaluation of the effects of human carcinogens and related chemicals on human foreskin fibroblasts. | ten compounds representative of diverse classes of chemicals were evaluated for their cytotoxicity and transforming ability to human skin fibroblasts in vitro. only five of the ten compounds were highly cytotoxic in the 0-100 micrograms/ml range and their order of cytotoxicity was: 2,5-bis(1-aziridinyl)-3,6-bis(carboethoxyamino)-1,4-benzoquinone (azq) greater than cis-platin greater than bis(chloromethyl)ether (bcme) greater than acrylonitrile greater than aflatoxin b1 (afb1). the other five com ... | 1990 | 2361190 |
| localization of dna in coxiella burnetii by post-embedding immunoelectron microscopy. | the deoxyribonucleic acid of coxiella burnetii was detected with monoclonal antibodies against single-stranded and double-stranded dna by post-embedding immunoelectron microscopy. the antibody labeled the nucleoid of the spore, the small cell variant (scv), and the large cell variant (lcv). the dna was segregated completely in both the spore and the mother cell. at the terminal stage of development of the spore, the loss of nuclear morphology in the mother cell was related to the reduction in th ... | 1990 | 2378445 |
| the immune response to phase i and phase ii coxiella burnetii antigens as measured by western immunoblotting. | to determine the utility of western immunoblotting in distinguishing chronic q fever from acute q fever, we first examined serum samples from individuals who had no antibodies to coxiella burnetii by either the indirect immunofluorescence antibody test or by the enzyme-linked immunosorbent assay. in these subjects, the igg fraction in low dilutions of serum (1:8, 1:16) reacted with as many as 10 proteins in phase i and phase ii antigens. this number of reacting bands seen in western blots was re ... | 1990 | 2378447 |
| rickettsia in texas. | since the first reported case in 1941, rocky mountain spotted fever in humans has been reported from many areas of texas, with two major foci, one located in the north-central region and the other in the eastern region of the state. during the period 1979-1988, 421 cases of rmsf were reported, reaching 108 cases in 1983 and declining in subsequent years. statewide surveillance programs to detect spotted fever group rickettsiae in tick populations were initiated in 1976. in recent years, the sfg ... | 1990 | 2378448 |
| q fever in octogenarians. | two of the 186 persons (1%) with acute q fever examined in this study were 80 years of age or older. both were males and both had coxiella burnetti pneumonia. q fever was not suspected during their hospital stay and would not have been diagnosed if these patients had not been part of a prospective study of community-acquired pneumonia. | 1990 | 2378454 |
| the role of lipopolysaccharides in the virulence of coxiella burnetii. | | 1990 | 2378455 |
| seroepidemiology of q fever among cats in new brunswick and prince edward island. | the indirect microimmunofluorescence test was used to determine the presence of antibodies to phase i and phase ii coxiella burnetii antigens in new brunswick and prince edward island cats. twenty of 104 (19.2%) new brunswick cats tested had antibodies to phase ii antigen; five of these (4.8%) also had antibodies to phase i antigen. six of 97 (6.2%) prince edward island cats tested had antibodies to phase i and phase ii antigens. our data suggest that cats may be important in the epidemiology of ... | 1990 | 2378456 |
| q fever in sokoto, nigeria. | there is very little information available about the prevalence of coxiella burnetii in africa. we obtained blood from 75 nigerians hospitalized in sokoto for a variety of acute medical conditions. their age range was 7 months to 50 years; there were 39 males and 36 females. antibody titers were determined to phase i and phase ii c. burnetii antigens using a microimmunofluorescence test. thirty-three (44%) had an antibody titer of greater than or equal to 1:8 to phase ii c. burnetii. we conclude ... | 1990 | 2378458 |
| occupational health guidelines for control of q fever in sheep research. | | 1990 | 2378459 |
| susceptibility of coxiella burnetii to chemical disinfectants. | | 1990 | 2378460 |
| unexpected antibiotic susceptibility of a chronic isolate of coxiella burnetii. | evidence is mounting in support of the idea that different isolates of coxiella burnetii, the etiologic agent of q fever, are responsible for the distinct disease syndromes observed clinically. recent studies have shown distinct antibiotic susceptibilities of different isolates of c. burnetii implicated in distinct clinical q fever syndromes. with this in mind, we performed antibiotic susceptibility testing of the "s" isolate, a chronic-type isolate retrieved from a human patient with chronic di ... | 1990 | 2378461 |
| antigenic structure of coxiella burnetii. a comparison of lipopolysaccharide and protein antigens as vaccines against q fever. | the antigenic structure of coxiella burnetii is being investigated by identifying both external and internal cellular epitopes of the morphologic cell types. both the phase i lipopolysaccharide (lpsi) and several surface proteins are candidates for the development of subunit multivalent vaccines. the protective efficacy of purified lpsi was demonstrated in a/j mice. the purified lpsi preparations contained residual peptides detected by amino acid analysis. therefore, the protection afforded by l ... | 1990 | 2378463 |
| the biology of coxiella burnetti and the pathobiochemistry of q fever and its endotoxicosis. | c. burnetii possesses a battery of host-independent enzymes which mediate endergonic and exergonic reactions. the biochemical and biophysical lesions responsible for the organism's obligate intracellular parasitic state have not been identified. clues to this fundamental problem may lie in the agent's acidophilic metabolism and proliferation within the host cell's phagolysomal vacuole. what are the modifiers of transcription and translation? what constituents of the lysosomal vacuole contribute ... | 1990 | 2378468 |
| dna probes for the identification of coxiella burnetti strains. | isolation of coxiella burnetii in the standard laboratory setting is hazardous; therefore most diagnoses are based on retrospective detection of a rising antibody titer to c. burnetti. as a result, this disease is usually undiagnosed or misdiagnosed. methods for the rapid detection of c. burnetti have now been developed that utilize specific hybridization of labeled dna probes to nucleic acid in clinical samples. one method detects the presence of c. burnetii 16s ribosomal rna (rrna); another us ... | 1990 | 2378470 |
| characterization of coxiella burnetii pyrb. | the c. burnetii pyrb gene was cloned on a 7-kbp ecor i fragment. dna sequence analysis, enzyme assays, and amino acid homologies with e. coli and b. subtilis pyrb gene products suggest that (i) c. burnetii atcase exists as a trimer, (ii) the microorganism may not synthesize a regulatory polypeptide, and (iii) pyrb may be part of an operon whose expression is under the control of an upstream promoter. the high degree of homology of the active site further suggests that a common mechanism of catal ... | 1990 | 2378471 |
| use of pulsed field gel electrophoresis to differentiate coxiella burnetii strains. | pulsed field gradient gel electrophoresis (pfge) provides a powerful technique for the analysis of bacterial genomic dna by allowing the resolution of dna fragments as large as 9000 kilobase pairs (kbp). when isolates of coxiella burnetii were examined using this method, the restriction enzymes not i and sfi i gave the fewest and most easily resolved fragments. sfi i cuts the genome of the priscilla isolate of c. burnetii into 15 dna fragments ranging in size from 320 to 18 kbp, and not i cuts t ... | 1990 | 2378472 |
| chronic q fever: diagnosis and follow-up. | sera from 40 patients (25 men, and 15 women) with clinical features compatible with the diagnosis of chronic q fever were received. total or partial clinical data were available. all of them had serological evidence of chronic q fever (igg class anti-phase i titer greater than 800). the final diagnosis was vascular infection in four cases (with two positive cultures for coxiella burnetii), bone infection in two patients (one positive culture), chronic hepatitis in one patient, and endocarditis i ... | 1990 | 2378473 |
| analysis of qprs-specific sequences from coxiella burnetii. | coxiella burnetii from acute cases of q fever possess a plasmid termed qph1. chronic isolates contain a plasmid termed qprs or have qprs sequences integrated into the chromosome. the correlation between an isolate's plasmid type and the chronic or acute nature of the disease has prompted analysis of unique plasmid sequences to determine if they contain virulence genes. dna hybridization has determined that a portion of a 3.6-kb ecor i fragment (epsilon') is unique to qprs. in vitro transcription ... | 1990 | 2378474 |
| comparison of coxiella burnetii plasmids to homologous chromosomal sequences present in a plasmidless endocarditis-causing isolate. | coxiella burnetii is the etiological agent of human q fever and chronic endocarditis. different plasmids have been found in c. burnetii isolates and a correlation between disease state and plasmid type has been established. the plasmid qprs was found in all but four of the endocarditis-causing isolates examined. these four isolates did not contain a detectable plasmid. however, when dna from the plasmidless isolates is hybridized with 32p-labeled qprs, homologous sequences are detected. it was h ... | 1990 | 2378475 |
| identification and cloning of a 27-kda coxiella burnetii immunoreactive protein. | patients with acute q fever develop coxiella burnetii-specific antibody, and chronic disease patients often develop extremely high levels of c. burnetii-specific antibody. antibody-reactive lps has been well characterized, but only a few immunoreactive proteins have been identified. an immunoreactive ca. 62-kda protein has been cloned, sequenced, and shown to be related to an escherichia coli heat-shock protein. a ca. 27-kda immunoreactive outer membrane protein(s) has also been identified. we h ... | 1990 | 2378476 |
| strategy for detection and differentiation of coxiella burnetii strains using the polymerase chain reaction. | a method for the rapid detection of coxiella burnetii and differentiation between strains that cause endocarditis and those that cause acute q fever is based on the observation that the different strains contain unique plasmid sequences. this method employs the polymerase chain reaction (pcr) and requires knowledge of specific dna sequences in the region (target) of dna to be amplified. to detect and differentiate between c. burnetii isolates, two sets of primers are required. the first set was ... | 1990 | 2378478 |
| endotoxin-induced uveitis in the rat: a hypothesis for preferential involvement of the anterior uvea. | endotoxin-induced uveitis (eiu) was produced in lewis rats by footpad injection of salmonella endotoxin. protein and cells were measured both in the aqueous humor and in the cerebrospinal fluid (csf) in order to examine if the inflammation was strictly limited to the anterior uvea. eiu was also induced in fischer and brown norway rats and the inflammation was compared among the three strains. although the function and structure of the choroid plexus is very similar to the ciliary body, no signs ... | 1990 | 2384004 |
| characterization and expression of the cbbe' gene of coxiella burnetii. | a gene which is unique to the qprs plasmid from chronic isolates of coxiella burnetii was cloned, sequenced, and expressed in escherichia coli. this gene, termed cbbe', codes for a putative surface protein of approximately 55 kda, termed the e' protein. the cbbe' gene is 1485 bp in length, and is preceded by predicted promoter regulatory sequences of tttaat (-35), tataat (-10), and a shine-dalgarno sequence of ggagaga, all of which closely resemble those of e. coli and other rickettsiae. the ope ... | 1990 | 2384744 |
| effect of neuromuscular blockade on fetal oxidative metabolism. | because fetal neuromuscular blockade has been used during cord blood sampling procedures to prevent fetal movement, we studied the effect of pancuronium bromide on fetal arterial concentrations of glucose, lactate, and oxygen and their umbilical uptake in 6 chronically prepared fetal sheep. we observed a significant increase in the arterial concentrations and decrease in the umbilical uptakes. we suggest that cord blood sampling procedures for fetal assessment of oxidative metabolism not be perf ... | 1990 | 2390540 |
| miller fisher syndrome associated with q fever. | a 51 year old woman with pneumonia developed miller fisher syndrome. indirect immunofluorescence tests showed antibodies against coxiella burnetti. miller fisher syndrome associated with q fever has not been described previously. | 1990 | 2391529 |