| transferable drug resistance of escherichia coli isolated from infant diarrhoea. | the resistance to several drugs was determined in 29 pathogenic strains of escherichia coli (026, 055 and 0111) isolated from infant diarrhoea and 18 non-pathogenic e. coli strains isolated from the same individuals. both pathogenic and nonpathogenic strains were resistant to at least 1 to 10 drugs, but only in four cases resistance patterns of the pathogenic strains were identical with those of non pathogenic ones. the majority of the strains were resistant to sulfonamide, tetracycline, ampicil ... | 1979 | 94745 |
| determination of metabolite compartition in hepatic cells by varying the redox state in vivo. | in the present study, metabolite (lactate, pyruvate, glycerol 3-phosphate, dihydroxyacetone phosphate) concentrations were measured in various redox states. the mathematical relations between metabolite concentrations in various redox states were expressed algebraically and studied. models which provided separate lactate/pyruvate (l/p) and glycerol 3-phosphate/dihydroxyacetone phosphate (g/d) spaces correspond to the experimental results in the case of "reductants" (e.g. ethanol, acetaldehyde, d ... | 1979 | 94822 |
| effect of beta-blockers on sodium and potassium content of human erythrocytes. | recently, hyperpotassemia was reported in patients treated with propranolol, but the mechanism has not yet been delineated. we have investigated the effects of various beta-adrenergic blockers and of d-propranolol on the na+ and k+ distribution intra- and extracellularly in human erythrocytes. k+ loss and na+ gain by cells was demonstrated at drug concentrations of 10(-4) m or greater. d-propranolol was more effective than l-propranolol, whereas pindolol was ineffective. practolol increased na+ ... | 1979 | 94824 |
| a research on the action of testosterone propionate and of ciproterone acetate on the mouse-killing behaviour of the adult rat. | data demonstrate exactly that testosterone favour above all the killing behaviour of castrate males at birth in comparison with control males while ciproterone acetate turns out to be more effective in diminishing the killing behaviour of males having integral testicle and of androgenized females at birth in comparison with castrated males. the results point out that the killing behaviour of rats is affected by the presence of testosterone in the first days of life, and in the grown-up animal. | 1979 | 94826 |
| antigenic homology of feline and human beta-hexosaminidase. | the immunological characteristics of feline beta-hexosaminidase (beta-d-n-acetylglucosaminidase, ec 3.2.1.30) isoenzymes, hex a and hex b, were studied. immunization of rabbits and goats with either cat hex a or hex b produced antibodies which reacted with a common antigenic marker shared by both hex a and hex b. with properly absorbed antisera, a unique antigenic marker was demonstrated on cat hex a, but not on hex b. this antigenic profile is comparable to that of the human beta-hexosaminidase ... | 1979 | 94833 |
| [effects of different concentrations of chloramphenicol on rna synthesis in e. coli]. | in the presence of 2 microgram/ml chloramphenicol the rate of total rna synthesis increases 1,6-fold, while that of rrna synthesis--2,5--3,0-fold. other ribosomal antibiotics, e.g. oxytetracycline, neomycin and puromycin, as well as chloramphenicol stimulate the synthesis of rna while not causing a complete inhibition of the protein synthesis. intensive stimulation of the rate of rrna synthesis with low concentrations of chloramphenicol may be due to a decrease of intracellular concentration of ... | 1979 | 94838 |
| [immunosuppressive activity of variously prepared alpha-globulin fractions of human blood]. | | 1979 | 94844 |
| [faecal enzyme diagnostic (author's transl)]. | the hitherto existing results of determinations of enzymatic activities in stool are presented in this review. the chymotrypsin activity is diminished in advanced exocrine pancreatic insufficiency. the faecal alpha-amylase activity has up to now no significance in the diagnosis of pancreatic diseases. up to five amylolytic enzyme activities are detectable. the alkaline phosphatase is mostly of intestinal origin. up to 4 enzyme bands can be exhibited with the disc electrophoresis in polyacrylamid ... | 1979 | 94852 |
| the effect of glutaurine on the development of amphibian larvae with inhibited rna and protein synthesis. | 1. glutaurine (gamma-l-glutamyl-taurine) isolated from bovine parathyroid and later synthetized retards the metamorphosis of rana arvalis. 2. from day 13 of treatment glutaurine moderates the metamorphosis inhibiting effect of antibiotics acting at the transcription and translation level. | 1979 | 94855 |
| [effect of ergoline derivatives on platelet and blood vessels]. | the influence of dihydrogenated ergot alkaloids on vessels and blood platelets was studied in vitro and in vivo. at low concentrations they cause contraction of isolated vein strips via a stimulation of alpha-adrenoceptors, at higher concentrations they antagonize the action of noradrenaline on arteries and veins and inhibit the adrenaline-induced platelet aggregation in vitro. in volunteers, intravenous and subcutaneous administration of dihydroergotamine (dihytamin) caused a decrease in blood ... | 1979 | 94864 |
| role of interferon in mice in protection against influenza a virus by bacterial ribosomes together with membranal glycoproteins of klebsiella pneumoniae as adjuvant. | nonspecific protection against infectious aerosols of influenza a virus was obtained in swiss mice after vaccination by aerosols of bacterial ribosomes together with membranal glycoproteins extracted from klebsiella pneumoniae as the adjuvant. it was shown that repeated stimulant aerosols were necessary to obtain this protection. routine estimation of serum interferon levels after administration of the association of ribosomes plus membranal glycoproteins to the animals by aerosol or intravenous ... | 1979 | 94908 |
| rapid isolation of carcinogen-bound dna and rna by hydroxyapatite chromatography. | carcinogen-bound dna and rna are conveniently isolated by solvent extraction and hydroxyapatite (hap) chromatography. tissue is suspended in 8 m urea-0.24 m sodium phosphate-1% sodium dodecyl sulfate-10 mm edta, ph 6.8 (mup-sds-edta) and extracted with chloroform-isoamyl alcohol-phenol (24:1:25; cip) to remove protein. rna and dna are separated by passing the aqueous solution through an hap column; rna is eluted with mup, dna with 0.48 m sodium phosphate, ph 6.8. examples presented are: (1) calf ... | 1979 | 94918 |
| [circadian variations of the plasma proteins in the adult male rat under natural synchronisation (author's transl)]. | circadian rhythms of blood total proteins, albumin, alpha 1-, alpha 2-, beta- and gamma-globulins were documented in 80 wistar spf adult male rats, synchronized by natural light (06.00-18.00 h) and darkness (18.00-06.00 h) during the month of october 78. blood was sampled at four fixed time points in the 24 h scale (i.e. 04.00, 10.00, 16.00 or 24.00 h). total proteins, albumin, alpha 2- and gamma-globulins showed a statistically significant rhythm with a maximum at 04.00 h. the data obtained in ... | 1979 | 94931 |
| electron microscopy of malachite green--glutaraldehyde fixed bacteria. | malachite green combined with glutaraldehyde has been used recently as a fixative for preserving and revaling lipid complexes in thin sections of eukaryotic cells examined by electron microscopy. when bacteria were prefixed with the above mixture granular electron dense inclusions were revealed in all cultures tested. these inclusions were replaced by electron transparent areas in cells fixed with glutaraldehyde alone. the structures were frequently located near to or within the nucleoid and adj ... | 1979 | 94953 |
| [interferon in the anti-influenza protection provided by a mixtures of bacterial ribosomes and a membrane fraction of k. pneumoniae applied as an aerosol]. | | 1979 | 94959 |
| synthetic antigens iii. antigen-binding cells to interpolymer of styrene and maleic acid psm and sheep red blood cells in mice immunized with psm. | studies on the immune response in mice to a new synthetic antigen interpolymer of styrene and maleic acid (psm) are described. the immune response was measured by determination of antigen binding cells by means of rosette test with psm-coated srbc, an unexpected effect was observed--besides rosette-forming cells for psm, rosette-forming cells and agglutinating antibodies for sheep red cells were found. the results presented in this paper suggest that psm is acting both as an immunogen and a poly ... | 1979 | 94989 |
| histochemical studies of mucosubstances and keratins of thymic corpuscles. | keratin and prekeratin have been visualized histochemically by testing the reactivity of disulfide groups in the thymic corpuscles of the sheep. rhesus monkey, rabbit and dog, and the reactivity of sulfhydryl groups in the sheep, rhesus monkey, rabbit and rat. ten to 50% of the thymic corpuscles showed keratinization by this method. acidic mucosubstances were visualized histochemically in the rhesus monkey, sheep and rat but not in the rabbit. staining for vic-glycol groups was prominent in all ... | 1979 | 94996 |
| pulsus alternans in regionally hypoxic ventricles of open-chest dogs: regional mechanical alternation of potentiation and attenuation of the inotropic state. | sustained mechanical alternation appeared after a spontaneous premature ventricular contraction in 6 open-chest dogs during the perfusion of the left circumflex coronary artery by krebs-henseleit solution equilibrated with 5% co2 and 95% n2. the animals were instrumented with a catheter in the left ventricle and ultrasonic crystals in hypoxic and control segments. left ventricular systolic pressure was significantly higher in the strong than in the weak beats with no significant difference of en ... | 1979 | 94997 |
| properties and usefulness of the original k-562 human myelogenous leukemia cell line. | | 1979 | 95026 |
| projections of raphe neurones to the cerebellar vermis cortex in the rabbit. a horseradish peroxidase (hrp) study. | | 1979 | 95045 |
| cerebral manifestations of whipple's disease. | a case of whipple's disease with central nervous system (cns) involvement has been retrospectively diagnosed. at the age of 50 and after six years of recurrent bouts of pyrexia, anorexia and loss of weight, a man developed a subacute encephalitis. two episodes of acute loss of vision were followed by changes in mental status, supranuclear ophthalmoplegia and oculo-facio-cervical myorhythmias which remained present until death, one year later. combined light- and electron microscopic studies have ... | 1979 | 95079 |
| biochemical and immunochemical studies of alpha-amylase from the salivary glands of the mouse. | | 1979 | 95093 |
| tumor immunology in domestic animals. | | 1979 | 95155 |
| [retrospective study in hepatic biopsies, of hepatitis b surface antigen by the orcein method and indirect immunofluorescence methods]. | a retrospective study of the hbsag was done in 56 liver biopsies of children less than 12 year-old and 78 biopsies of adults. the study was performed by orcein stain and indirect immunofluorescent method. in 23 of the adults patients, the serological detection of hbsag and antibodies (hbsab) was determined by reverse passive haemagglutination technique. the adults patients' histological dianosis were variable and included acute or chronic hepatitis (20.5%) and cirrhosis (24.4%). orcein was posit ... | 1979 | 95161 |
| visualization of mitochondrial coupling factor f1(atpase) by freeze-drying. | it is known that the negatively stained preparations of inner mitochondrial membrane display characteristic approximately 9 nm f1 (atpase) knobs projecting from the matrix surface. freeze-etch studies have reported the absence of such knobs from the "etched" surface of the inner mitochondrial membranes. we have demonstrated their presence on the surface of smp (submitochondrial particles) prepared by freeze-drying for transmission electron microscopy. this identification has been substantiated b ... | 1979 | 95168 |
| effects of ovarian hormones on cell membranes in the rat uterus. i. freeze fracture studies of the apical membrane of the liminal epithelium. | freeze fracture techniques have been used to study the apical membrane of cells of the luminal epithelium of the rat uterus under various hormonal regimes. in the ovariectomized but otherwise untreated rat, intramembranous particles (imps) occur at a density of 1395 +/- 122 per micron 2; they appeared spherical and uniformly distributed. after 3 days treatment with estrogen, no change in appearance or density was found, but 3 days of progesterone treatment produced a significant increase in imp ... | 1979 | 95176 |
| isoprinosine increases the antitumor action of interferon. | the association of relatively low doses of interferon with an immunostimulant, isoprinosine, enhanced the antitumor effect of interferon. each mouse was inoculated with 10(6) crocker tumor 180/tg cells. the best results were obtained when both interferon and isoprinosine were injected three times a week for 1 month. under these conditions, the mean survival time increased from 26 days in the controls to 45 days in the interferon-treated group to 64 days when both agents were used. isoprinosine a ... | 1979 | 95187 |
| immunochemical identity of "decidualization-associated protein' and alpha 2 acute-phase macroglobulin in the pregnant rate. | | 1979 | 95199 |
| experimental determination of interacting sequences in ribosomal rna. | | 1979 | 95206 |
| inhibition and stimulation of histamine release by oxatomide. | | 1979 | 95250 |
| chromatographic properties of sephacryl s-300 superfine. | sephacryl s-300 superfine is a new gel filtration medium that consists of allyldextran crosslinked with n,n'-methylenebisacrylamide. it was shown to have a separation range for proteins that extends to 1--2 x 10(6) dalton. the gel filtration properties were characterized by fractionation of human serum and of bovine colostrum. thyroglobulin from pig could be isolated in one step in a very pure form. the flow properties were characterized by the relationship between flow rate and pressure drop. i ... | 1979 | 95254 |
| spontaneous termination of reentry ventricular tachycardia in the late myocardial infarction period: an experimental study in the dog. | forty episodes of induced ventricular tachycardia in the late myocardial infarction period (4-6 days old) were analyzed in 12 dogs in an attempt to identify the possible mechanisms for the termination of reentry tachycardia. multiple epicardial and endocardial composite electrograms were recorded in and around the central ischemic zone of the infarction. during tachycardia, the earliest site of activation was identified in the epicardial surface of the border or normal zone immediately adjacent ... | 1979 | 95291 |
| a rat model (using a semipurified diet) of the fetal alcohol syndrome. | a semipurified liquid diet was used to develop a chronic rat model of the fetal alcohol syndrome. control female rats gained weight normally, reproduced normally, and gave birth to normal litters on this diet. increased neonatal mortality, decreased neonatal weights, and altered sex ratios were observed in offspring of experimental alcoholic animals. preliminary histological results include a mild delay in cell lamination patterns in the cerebral cortex at 4 days postnatal in alcoholic offspring ... | 1979 | 95338 |
| antigenic similarity between squamous cell carcinoma of horn (horn cancer) and normal bovine foetal tissues. | normal bovine foetal (liver and skin) and horn cancer tissue antigens were examined using double diffusion agar gel precipitation and immuno-electrophoretic tests to detect any cross reactivity among them. rabbit horn cancer antisera absorbed with normal bovine liver, skin and horn core epithelium antigens, when tested with foetal skin and liver (4 to 6 months of gestation), revealed the presence of 2 foetal antigens in horn cancer. immuno-chemically 2 of the horn cancer antigens were found to b ... | 1979 | 95358 |
| [adaptation of the cardiac cell to chronic mechanical overload]. | | 1979 | 95439 |
| flow cytometry: general principles and applications to selected studies in tumor biology. | the cell populations derived from normal tissues and solid tumors comprised many different cell types. within each cell type there is a distribution of cells in different phases of the cell cycle and/or metabolic states (ie, differing rates of protein, rna, and other macromolecular syntheses). flow cytometry and companion instrumentation now promise to aid in rapid quantitative analyses of heterogeneous cell populations, thus finding broad applicability in many areas of cancer research and treat ... | 1979 | 95452 |
| [nucleic acids in the liver of rat treated with "e. coli" o 127 lipopolysaccharide (author's transl)]. | the authors have studied the variations of nucleic acids, proteins and histological aspect in the liver of rats, treated with 1 mg of e. coli ) 127 lipopolysaccharide and killed at 24th, 48th, 72th, 96th hour after the inoculation. the endotoxin determines an increase of nucleic acids and a reduction of hepatic proteic contents. the histological observation shows throw-back phenomenons of the hepatocyte with hyperplasy and hypertrophy of kupffer cells at 24th hour, while at following intervals t ... | 1979 | 95476 |
| in vivo and in vitro reactions to antigens of haemophilus influenzae in bronchial obstruction. | the frequency of precipitating antibody to heat-labile (h(1--2) and heat-stable (hcw and hcf) antigens of haemophilus influenzae was determined in patients with asthma, chronic bronchitis, cystic fibrosis and bronchiectasis and compared with that in a control group. this showed that the immune response of asthmatic patients to heat-stable antigens was different from that to the heat-labile antigens. exposure to antigens of h. influenzae is common in all the disease groups. skin test reactions ha ... | 1979 | 95481 |
| lipophilic o-antigens in rhodospirillum tenue. | lipopolysaccharides of eight wild-type strains of the phototrophic bacterium rhodospirillum tenue have been analyzed. all of the lipopolysaccharides are highly lipophilic. the compositions of preparations obtained by the phenol-water or by the phenol-chloroform-petroleum ether procedure are very similar. the polysaccharide moiety, obtained by mild acid hydrolysis of lipopolysaccharide, consists mainly of aldoheptoses: l-glycero-d-mannoheptose is present in all strains, whereas d-glycero-d-mannoh ... | 1977 | 95659 |
| formation of hcg in the human fetoplacental unit. | hcg and beta-hcg were determined in umbilical arterial and venous blood immediately after spontaneous term delivery. hcg and beta-hcg are present in higher concentrations in arterial than in venous umbilical cord blood. the data are consistent with the hypothesis that hcg and beta-hcg are produced in the fetal compartment of the human fetoplacental unit, with a larger contribution of the female fetus as compared with the male. | 1979 | 95667 |
| mediators of immediate-type hypersensitivity in sputum from patients with chronic bronchitis and asthma. | mediators of immediate-type hypersensitivity were studied in the sputum of patients with chronic bronchitis. the same mediators were also measured in early-onset, skin-test-positive asthmatics, in late-onset, skin-test-negative asthmatics, and in patients with bronchial carcinoma, bronchiectasis, and pneumonia. sputum eosinophilia was a feature of bronchitics and asthmatics, whereas raised blood eosinophil levels were found only in the early-onset, skin-test-positive asthmatics. histamine and ig ... | 1977 | 95732 |
| determination of the human lymphokine leukocyte migration inhibitory factor (lif) by a sensitive radioenzymatic assay. inhibitory effect of cgmp on the esterolytic activity of highly purified lif. | indirect experiments using irreversible enzyme inhibitors have shown that the human lymphokine leukocyte migration inhibitory factor (lif) is a serine esterase and protease exhibiting specific affinity towards arginine esters and amides. a sensitive assay for direct measurement of esterase activity using p-tosyl-l-arginine (3h) methyl ester (3h-tame) as substrate is described. esterolytic activities are demonstrated in crude supernatants of human lymphocytes stimulated with concanavalin a (lif-r ... | 1979 | 95804 |
| [the preservation of the causative agent of intestinal yersiniosis in xenopsylla cheopis fleas]. | experimentally infected fleas of x. cheopis are capable of preserving two strains of y. enterocolitica. one of the strains was found to belong to the 3rd, the other to the 9th serotype. the both strains actively multiplied and accumulated in great amount (up to several millions of microbial cells) in ectoparasites which were maintained at 6 to 8 degrees c. at a temperature of 18 to 21 degrees c some part of fleas gradually became free from the agent that was favoured by their periodical feeding ... | 1979 | 95814 |
| [external brain acetylcholinesterase in the ontogeny of precocial and altricial rodents]. | the share of external acetylcholinesterase (ache) has been determined in different brain structures of maturely and immaturely born rodents. it was found that in maturely born animals (guinea pigs) the share of external ache in all the studied brain structures is the same in newborn and adult individuals. in immaturely born animals (albino rats) the share of external ache to the moment of birth is significantly higher as that in adult organisms. during the development of the brain, the share of ... | 1979 | 95839 |
| inhibition of leucyl-trna synthetase in escherichia coli by the cytostatic 5,8-dioxo-6-amino-7-chloroquinoline. | at concentrations of 1-1.6 mug/ml, 5,8-dioxo-6-amino-7-chloroquinoline causes auxotrophy for leucine in escherichia coli mre 600. with increasing concentrations of this quinone additional amino acids are required for growth. the amount of leucine in the pool of free amino acids is not decreased after treatment of e. coli with the quinone. transfer rnaleu, however, is charged with leucine less than 10% in quinone-treated cells of e. coli, whereas in control cells the degree of aminoacylation is a ... | 1975 | 95889 |
| [morphology of ribosomal rna in chicken fibroblasts]. | it is shown with the electron microscope that the 28 s rna component of the ribosomal rna extracted from chicken fibroblasts contains secondary structures which are not present in the 18s component. | 1978 | 95896 |
| [demonstration of erythroblastic differentiation after 14 days in blood in vitro during vaquez's disease without addition of erythropoietin]. | leucocytes of normal individuals and patients with polycythemia vera were isolated from the peripheral blood by ficoll-hipaque density gradient centrifugation and cultured in vitro suing the bovine plasma clot culture technique with a minor modification: the addition of fresh normal serum. after 14 days in the presence of sheep erythropoïetin (3u/ml) erythropoïetic bursts containing between 3 and 10 subcolonies were observed in normal and polycythemia vera cultures. blood leucocytes of patients ... | 1978 | 95898 |
| the effect of blood on ocular fundus reflectance and determination of some optical properties of retinal blood vessels. | light reflected from 50 micrometer diameter spots on the fundi of two adult rhesus monkeys was measured as a function of wavelength in 10 nm jumps from 400 to 900 nm. the areas measured were a retinal artery and vein, areas of the disk, macula, and retina devoid of visible blood vessels, and the foveola. the eyes of the two monkeys were then exsanguinated, the blood vessels were filled with normal saline, and reflectances of the same spots on the fundi were again measured. the pairs of reflectan ... | 1978 | 96040 |
| comparison of the effect of vasopressin infusions on the mesenteric arteries of different species. | superior mesenteric arteriography was performed following infusions of vasopressin, epinephrine and saline into the superior mesenteric arteries of dogs, pigs, rabbits and monkeys and compared with the well-known effects in human beings. species-specific effects were noted and compared with the known differences in various vascular beds in human beings. in non-primate animals, vasopressin appeared to act at a more distal site than in primates, but with a similar decrease in superior mesenteric a ... | 1978 | 96042 |
| tumor biology of helium and heavy ions. | | 1977 | 96044 |
| relationship between cellular autolytic activity, peptidoglycan synthesis, septation, and the cell cycle in synchronized populations of streptococcus faecium. | synchronized, slowly growing (td = 70 to 80 min) cultures were used to study several wall-associated parameters during the cell cycle: rate of peptidoglycan synthesis, septation, and cellular autolytic activity. the rate of peptidoglycan synthesis per cell declined during most of the period of chromosome replication (c), but increased during the latter part of c and into the period between chromosome termination and cell division (d). an increase in cellular septation was correlated with the inc ... | 1978 | 96095 |
| tryptophan residues of saccharifying alpha-amylase from bacillus subtilis. a kinetic discrimination of states of tryptophan residues using n-bromosuccinimide. | four tryptophan residues of saccharifying alpha-amylase from b. subtilis out of eleven in total are reactive towards n-bromosuccinimide (nbs), suggesting that they are on the surface of the enzyme. this is consistent with the results of solvent perturbation difference spectrophotometry with ethylene glycol. one of four tryptophan residues was clearly distinguished from the other three in reactivity with nbs by the stopped-flow method. this most reactive tryptophan residue was not protected from ... | 1978 | 96111 |
| naturally occurring diatomaceous pneumoconiosis in sub-human primates. | | 1978 | 96157 |
| laboratory investigations of an epidemic of acute haemorrhagic conjunctivitis in bombay and poona in 1975. | | 1978 | 96187 |
| l-alanyl-l-tyrosine as a tyrosine source during intravenous nutrition of the rat. | tyrosine is considered an essential amino acid for some premature infants, but its limited solubility prevents addition to intravenous solutions in adequate amounts. tyrosine peptides with solubilities greater than tyrosine were synthesized to evaluate potential for intravenous use. intraperitoneal injection of l-alanyl-l-[u-14c]tyrosine (0.5 mmoles/kg) into adult rats resulted in a rapid labeling of tissue pools and production of 14c-co2. when the peptide was infused as part of an intravenous n ... | 1978 | 96233 |
| [pathogenicity of the indigenous flora of the oral cavity]. | | 1977 | 96398 |
| [the appearance of penicillin resistant gonococci. clinical, genetic and epidemiologic problems]. | | 1978 | 96408 |
| [tuberculin reactions in guinea pigs injected with virulent and avirulent tubercle bacilli]. | | 1978 | 96433 |
| pre-proparathyroid hormone; amino acid sequence, chemical synthesis, and some biological studies of the precursor region. | the precursor of bovine proparathyroid hormone was synthesized by translation of parathyroid mrna in a wheat-germ cell-free system. the amino acid sequence of the nh2-terminal extension (the pre sequence) was determined by repetitive edman degradation of the polypeptide labeled with radioactive amino acids (radiosequencing). the pre sequence of pre-proparathyroid hormone is (formula: see text) which is followed by the sequence of proparathyroid hormone. it is significant that 20 of the 25 amino ... | 1978 | 96437 |
| chromosomal locations of mouse immunoglobulin genes. | the chromosomal locations of the structural genes coding for the constant portions of mouse heavy (h) and light chain immunoglobulins were studied by molecular hybridization techniques. complementary dna probes containing the constant-region sequences of kappa and lambdai light chain and alpha, gamma2b, and mu heavy chain mrnas were annealed to a large excess of dna from a series of eight mouse-human hybrid cell lines that are deficient for various mouse chromosomes. the lines were scored as pos ... | 1978 | 96442 |
| direct demonstration of immunoglobulin kappa chain rna in thymus t cells by in situ hybridization. | mouse thymuses with more than 99% t cells have been reported to contain immunoglobulin kappa mrna-like molecules (kappa rna) in relatively large quantities. the present study was undertaken to rule out the possibility that the kappa rna was mainly a product of a few contaminating b cells of the thymus and to determine whether all t-cell subpopulations contained kappa rna. by in situ hybridization with dna complementary to kappa mrna (kappa cdna) the following observations were made: 98.5% of thy ... | 1978 | 96443 |
| the effects of methylphenidate on repeated acquisition of serial discrimination reversals. | rats were trained to bar-press for sucrose solution in the presence of one of two stimulus conditions. on each daily training session, the stimulus during which bar-pressing was reinforced was reversed. the subjects were trained in this serial discrimination reversal procedure until successive acquisitions of the discrimination had stabilized. drug treatments consisting of saline or 0.25, 1.0, 2.0, 4.0, 6.0 or 8.0 mg/kg of methylphenidate were then administered 20 min prior to the daily training ... | 1978 | 96462 |
| effect of intracerebroventricular bradykinin, angiotensin ii, and substance p on multiple fixed-interval fixed-ratio responding in rabbits. | | 1978 | 96474 |
| a comparison of the effects of 50 mevd leads to be neutron and cobalt-60 irradiation of the kidneys of rhesus monkeys. | twenty rhesus monkeys had one kidney irradiated (after undergoing unilateral nephrectomies) with one of four doses: 960 or 1080 rads of 50 mevd leads to be neutrons, or 2350 or 2700 rads of 60co. whereas animals treated with the lower dose of neutrons or 60co are alive with relatively normal renal function, those treated with the higher dose of neutrons died of radiation nephritis. animals treated with the higher dose of 60co developed radiation nephritis but survived. the physiological and hist ... | 1978 | 96490 |
| chelation in metal intoxication. vi. influence of pas and cdta on the excretion of manganese in rabbits given mno2. | the success of p-aminosalicyclic acid (pas) and 1,2-cyclohexylene dinitrilotetraacetic acid (cdta) in the mobilization of manganese from some vital organs and their subcellular fractions of manganese-intoxicated rats, led to the two compounds beng further investigated individually and in combination for their efficacy in influencing excretion of manganese in rabbits given mno2. these chelating agents led to a higher excretion of manganese through faeces than urine. whereas cdta was able to enhan ... | 1978 | 96549 |
| [diagnosis of vibriosis in bulls at breeding stations through lavages of the artificial vagina]. | the objective of the study was to test the suitability of lavages of artificial vaginas which are used for collecting ejaculate for diagnosing the campylobacter fetus subsp. fetus germs. according to the results of the investigation this method may be considered equal to the method of lavages of the prepuce. however, it is important to point out the advantages: it eliminates the danger of an accident, it enables quality lavage in the laboratory. to caputure the campylobacter fetus the filtration ... | 1978 | 96573 |
| [study of the spiral and spindle-shaped formations in cultures of bacillus larvae (white 1906) causing foulbrood in bees]. | the authors have been studying the bacterial diseases of the brood for a fairly long time. american and european foul brood has been studied with the highest attention. cultures of bacillus larvae (white, 1906) were examined both in freshly isolated strains and in collection strains of this micro-organism. in cases of foul brood, the pathological material was found to contain not only the typical rods of b. larvae but also immobile spiral forms which are usually referred to in literature as frag ... | 1978 | 96576 |
| a sarcocystis-like protozoon in a sheep with lymphadenopathy and myocarditis. | a generalized enlargement of the lymph nodes was found in an emaciated adult ewe. additional autopsy findings included tiny grey-white necrotic foci in the heart muscle, aspiration pneumonia and diffuse pleuritis. light microscopy showed a generalized lymphadenopathy with perilymphadenitis, depletion of lymphocytes and histiocytosis of the lymph node. in histiocytes and vascular endothelial cells of lymph nodes, septal capillary endothelium of lungs and capillary endothelium of myocardium, early ... | 1978 | 96578 |
| development of visual acuity in infant monkeys (macaca nemestrina) during the early postnatal weeks. | | 1978 | 96592 |
| [hyaluronidase in meningococcus]. | a total of 204 meningococcus strains were tested for the presence of hyaluronidase, and 45.5% of the strains were found to contain it. strains penetrating into the cerebrospinal fluid were the ones which largely produced the enzyme (in 83% of the cases). the enzyme was revealed only in 25.5% of the strains habituating on the nasopharyngeal mucosa. hyaluronidase was mostly found in the meningococcus strains referred to the serological group a; strains of other serological groups and ungrouped str ... | 1978 | 96634 |
| [participation of r plasmids in the transfer of chromosome genes]. | | 1978 | 96640 |
| pituitary-adrenal response to stimulation of the limbic system and lateral hypothalamus in the rhesus monkey (macacca mulatta). | various sites within the limbic system, frontal lobe and lateral hypothalamus of the rhesus monkey brain were electrically stimulated using chronically implanted electrodes. increases in plasma cortisol was observed after stimulation of the lateral hypothalamic area, basolateral amygdala, pyriform cortex, hippocampus, certain sites in the cingulate gyrus and orbital part of the frontal lobe. inactive sites included the caudate nucleus, putamen and white matter in the frontal lobe. | 1978 | 96644 |
| electron-microscopic studies on primate neurons: microtubule-pigment associations. | the relationship between microtubules and the lipoprotein pigment found in senile cynomolgus brain and senile human brain biopsy material was investigated. numerous microtubules were present in all parts of the cytoplasm and within the pigment areas, running parallel or obliquely to the pigment bodies and being associated with its lateral aspects. microtubules also occurred in the periphery of neurons or appearing to enter the perineuronal oligodendrocytes. these observations indicate a possible ... | 1978 | 96651 |
| the double flash erg during adaptation to the dark. | electroretinogram (erg) of albino rabbits and rheusus monkeys were recorded in a dark room applying the double flash stimuli after a sufficient adaptation to light. the stimulus intervals of the flashes were varied. the per cent amplitudes of the ergs for the first flashes (b1) and those of the second flashes (b2) were plotted against time, as was (b1-b2)/b1 (=k). 1. b1 was independent of the stimulus interval in both species, and increased with lapse of time to reach a plateau. 2. b2 was quite ... | 1978 | 96656 |
| animal-human correlates of narcotic dependence: a brief review. | knowledge of opiate action in laboratory animals can be of great value in developing a rational clinical approach to the treatment of narcotic dependence. physiological alterations experimentally produced by narcotics have been confirmed in human subjects, and classical laboratory effects of conditioning and operant behavior also have clinical counterparts. realization of animal-human correlates of narcotic dependence allows a better understanding of the strengths and weaknesses of existing ther ... | 1978 | 96703 |
| tonic retinal influences in primates. | a type of unit discharge, termed "luxotonic," has been found in the striate cortex of unanesthetized squirrel monkeys and macaques.6, 21 the firing frequency of these units shows relatively little adaptation, continues indefinitely (hours), and reflects the level of diffuse illumination of the eye. the more numerous "photergic" units discharge more rapidly in the light, whereas "scotergic" units fire fastest in the dark (or at luminance levels below threshold for cones). luxotonic activity is ab ... | 1977 | 96720 |
| somatosensory restriction and behavioral development in stumptail monkeys. | | 1977 | 96721 |
| activity of azlocillin and mezlocillin against gram-negative organisms: comparison with other penicillins. | the activities of azlocillin and mezlocillin were compared with those of carbenicillin, ticarcillin, and pirbenicillin against a wide range of gram-negative organisms. the two new drugs were considerably more active than carbenicillin against klebsiella species and escherichia coli. carbenicillin was twice as active against proteus mirabilis as mezlocillin and four times as active as azlocillin. against pseudomonas aeruginosa, azlocillin was eight times as active as carbenicillin. azlocillin and ... | 1978 | 96726 |
| sk&f 75073, new parenteral broad-spectrum cephalosporin with high and prolonged serum levels. | sk&f 75073, a new parenteral cephalosporin, was found to have broad in vitro and in vivo antibacterial activity including isolates usually resistant to cephalothin and cefazolin. this activity included indole-positive proteus and enterobacter species and some serratia isolates. proteus mirabilis strains were particularly susceptible, as were haemophilus influenzae and neisseria species. the activity of sk&f 75073 against gram-positive bacteria was poorer than that of the control cephalosporins. ... | 1978 | 96734 |
| unpredictable response of pseudomonas aeruginosa to synergistic antibiotic combinations in vitro. | the combined activity of ticarcillin and each of three aminoglycosides, gentamicin, tobramycin, and amikacin, was studied with 45 clinical isolates of pseudomonas aeruginosa. all strains individually were susceptible to each of these agents. comparison of the results revealed highly variable interaction, both from strain to strain and from one aminoglycoside to another against the same strain. against 11 strains, none of three antibiotic pairs tested was synergistic by conventional criteria; aga ... | 1978 | 96736 |
| heterocomplexes of human interferon and immunoglobulins: formation and properties. | to form heterocomplexes, human diploid fibroblast interferon was reacted with an excess of human immunoglobulin in the presence of an optimal concentration of glutaraldehyde. there was virtually no loss of interferon activity due to this treatment and the resulting heterocomplexes possessed the biological properties of both native interferon and immunoglobulin molecules. the apparent molecular weights determined by gel electrophoresis in the presence of sodium dodecyl sulfate were 20,000 for nat ... | 1978 | 96781 |
| effect of oophorectomy on estrogen receptors in rat mammary tumors induced by 7,12-dimethylbenz (a)anthracene. | | 1977 | 96813 |
| interaction of the nitrogenase components of anabaena cylindrica with those of clostridium pasteurianum. | | 1978 | 96817 |
| 5-thio-d-glucose is an acceptor for udp-galactose : d-glucose 1-galactosyltransferase. | | 1978 | 96819 |
| skunks have gene sequences in their cellular dna related to squirrel monkey retrovirus: transmission between species of a new world primate endogenous type d retrovirus. | | 1978 | 96832 |
| indirect chromosomal fluorescence with antibody to 7, 12-dimethylbenz(a)anthracene. | | 1978 | 96836 |
| genetics of complement deficiencies associated with lupus-like syndromes. | there appears to be an association between deficiencies of a number of complement components, particularly of the second component (c2), and rheumatic diseases, especially lupus. the meaning of this association is not clear, but the linkage of deficiency of c2 with hla, especially hla-a10, b18, dw2, as well as with bfs, suggests a possible linkage to immune response genes. | 1978 | 96843 |
| differential effects of prostaglandin synthetase stimulators on inhibition of cyclooxygenase. | the different effects of prostaglandin synthetase stimulators on inhibition of the cyclooxygenase by structurally distinct classes of nonsteroidal antiinflammatory agents suggest that the enzyme is altered by interaction with these stimulators. reversible stimulation of prostaglandin synthetase activity by phenols and some other compounds and the relative influence of these stimulators on inhibitors of the cyclooxygenase were determined quantitatively. two distinct classes of inhibitors were est ... | 1978 | 96853 |
| phycobiliprotein synthesis in protoplasts of the unicellular cyanophyte, anacystis nidulans. | stable and metabolically active protoplasts were prepared from the unicellular cyanophyte, anacystis nidulans, by enzymatic digestion of the cell wall with 0.1% lysozyme. the yield of protoplasts from intact algal cells was approx. 50%. incorporation of l-[u-14c]leucine into cold trichloroacetic acid-insoluble material from protoplasts preparations was linear for 1.5 h and continued for an additional 2.5 h. incorporation of radiolabeled leucine into hot trichloroacetic acid-insoluble material fr ... | 1978 | 96857 |
| comparison of effects of normal rabbit sera and anti-cock sperm sera on rabbit sperm, including comparison of effects on the sex ratio. | | 1978 | 96880 |
| fibrinogen and adp-induced platelet aggregation. | | 1978 | 96891 |
| metabolism studies with practolol [proceedings]. | | 1978 | 96899 |
| effects of thyrotropin releasing hormone, norepinephrine and acetylcholine on the activity of neurons in the hypothalamus, septum and cerebral cortex of the rat. | | 1978 | 96915 |
| bovine transferrin glycopeptide: the relevance of its structure to interaction with the mammalian hepatic lectin that binds asialoglycoproteins. | | 1978 | 96919 |
| experimental polychlorinated biphenyl toxicosis in germfree pigs. | the effects of polychlorinated biphenyls were studied in eight germfree pigs. beginning at fourteen days of age, two pigs each were fed daily 12.5, 25, 50 and 100 mg/kg body weight of polychlorinated biphenyls as aroclor 1254. three germfree pigs were negative controls. clinically the treated pigs had inappetance, a hemorrhagic diarrhea, erythema of the nose and the anus, retarded growth, distended abdomen and at the higher dose levels, incoordination and coma followed by death. deaths occurred ... | 1978 | 96924 |
| test for differentiation of m. tuberculosis and m. bovis from other mycobacteria. | a test is described for selective inhibition of mycobacterium tuberculosis and m. bovis isolates in fluid medium. the method employs rho-nitro-alpha-acetyl-amino-beta-hydroxy-propiophenone (nap) as an inhibitory agent for differentiation of mammalian tuberculosis strains from other mycobacteria. | 1978 | 96930 |
| [chemical reactivity and biological properties of a series of aminochlorambucil derivatives]. | the substitution of aminochlorambucil by a methyl group increased the chemical reactivity in iv b (n = 1) and iv b (n = 2) but their cytotoxicity remained low. the immunosuppressive effect (adjuvant arthritis in rats and tuberculin related skin reaction) was observed with iv b (n = 2). aminochlorambucil was effective on adjuvant arthritis only and iv b (n = 1) had no activity. aminochlorambucil, iv b (n = 1) and iv b (n = 2) were devoid of any non-specific anti-inflammatory activity. | 1978 | 96958 |
| [permissivity of the rat (rattus rattus) of guadeloupe with regard to schistosoma mansoni and evidence for migration of adult schistosomes to the lungs]. | in contrast to the laboratory rat, the rat (rattus rattus) of guadeloupe is a favorable host for the life cycle of s. mansoni: the parasite population is maintained at a high level (between 8 and 16% in this host until the 16th week and reproduces normally). the evolution of the parasitose is marked, shortly after the 8th week, by a transfer of schistosomes from the liver to the lungs. | 1978 | 96960 |
| [toxicity of bacillus thuringienses var. israelensis for larvae of aedes aegypti and anopheles stephensi]. | the comparative study of the larvicidal action of b. thuringiensis var. israelensis on a. aegypti and a. stephensi shows the greater sensitivity of a. aegypti, with 100% of mortality in 30 to 40 min. at high doses. but, for both species of mosquito, the toxicity of these bacteria is very high, as shown by the mortality regression curves and by the lc50. this toxicity is associated with the crystals and can be extracted from them by dilute alkali solution, like the general case of the other serot ... | 1978 | 96979 |
| [lymphocyte subpopulations in adjuvant arthritis of rats. effects of corticoids and gamma irradiation]. | experimental model of human chronic inflammatory arthritis, adjuvant arthritis may be induced only in several strains of inbred rats: it is well developed in lew and practically absent in wag. after adjuvant injection, the pha-stimulable lymphocytes subpopulation quite disappears from the blood, if polyarthritis is well developed. these cells are probably capted in the tissues implicated in immunological conflict. on the contrary, the cona-stimulable subpopulation is enhanced in both strains aft ... | 1978 | 96995 |