| susceptibility to varicella-zoster virus among adults at high risk for exposure. | the adult health care provider who is susceptible to varicella zoster virus (vzv) represents a risk to her or himself and to patients. nineteen percent of employees at this children's hospital had no or uncertain prior experience with vzv, and of these, 28% were found to be vzv susceptible, representing 5% of the total population of 2,730 hospital employees. during the 12 months of study, six of the potential 135 to 137 vzv-susceptible individuals acquired varicella. vzv-susceptible health care ... | 1986 | 3019915 |
| a mutant human proinsulin is secreted from islets of langerhans in increased amounts via an unregulated pathway. | a coding mutation in the human insulin gene (his-b10----asp) is associated with familial hyperproinsulinemia. to model this syndrome, we have produced transgenic mice that express high levels of the mutant prohormone in their islets of langerhans. strain 24-6 mice, containing about 100 copies of the mutant gene, were normoglycemic but had marked increases of serum human proinsulin immunoreactive components. biosynthetic studies on isolated islets revealed that approximately 65% of the proinsulin ... | 1988 | 3057496 |
| a mutant human proinsulin is secreted from islets of langerhans in increased amounts via an unregulated pathway. | a coding mutation in the human insulin gene (his-b10----asp) is associated with familial hyperproinsulinemia. to model this syndrome, we have produced transgenic mice that express high levels of the mutant prohormone in their islets of langerhans. strain 24-6 mice, containing about 100 copies of the mutant gene, were normoglycemic but had marked increases of serum human proinsulin immunoreactive components. biosynthetic studies on isolated islets revealed that approximately 65% of the proinsulin ... | 1988 | 3057496 |
| probable detection of kil peptide derived from colicin e1 plasmid in the envelope fraction of escherichia coli hb101 carrying peap31. | escherichia coli carrying plasmid peap31 produces extracellularly alkalophilic bacillus penicillinase encoded on the plasmid. the extracellular production has been suggested to be caused by activation of dormant colicin e1 kil gene. two peptides that could be respectively precursor and mature products of colicin e1 kil gene were detected on an sds/polyacrylamide gel. one of the peptides (mr 4800), which was probably a precursor peptide, was detected in the inner-membrane fraction from the organi ... | 1988 | 3058121 |
| probable detection of kil peptide derived from colicin e1 plasmid in the envelope fraction of escherichia coli hb101 carrying peap31. | escherichia coli carrying plasmid peap31 produces extracellularly alkalophilic bacillus penicillinase encoded on the plasmid. the extracellular production has been suggested to be caused by activation of dormant colicin e1 kil gene. two peptides that could be respectively precursor and mature products of colicin e1 kil gene were detected on an sds/polyacrylamide gel. one of the peptides (mr 4800), which was probably a precursor peptide, was detected in the inner-membrane fraction from the organi ... | 1988 | 3058121 |
| high-molecular-weight neutral proteinase of rat ascites hepatoma cells and their collagenolytic activity. | a high-molecular-weight proteinase in rat ascites hepatoma ah109a cells was purified to apparent homogeneity by conventional chromatographic techniques. the purified proteinase degraded over the broad ph range, 7.0-9.0, not only denatured hemoglobin but also type i and iv collagen in the absence of calcium. its activity was maximum at ph 8.0, heat-labile and affected by thiol reagents. serine proteinase inhibitors such as diisopropyl fluorophosphate, soybean trypsin inhibitor, leupeptin, and ant ... | 1988 | 3058624 |
| vitamin e deficiency and neurologic disease. | during the recent resurgence of interest in the clinical uses of vitamin e, one of the major foci of attention has been the neurologic role of vitamin e in humans. studies in patients with secondary vitamin e deficiency, caused by fat malabsorption disorders and total parenteral nutrition lacking an adequate supply of vitamin e, have elucidated a clinical disorder and histologic lesions of the nervous system and muscle that closely resemble those of experimental vitamin-e-deficient animal models ... | 1988 | 3060170 |
| what schedule-induced polydipsia can tell us about alcoholism. | an animal model of chronic and excessive voluntary (unforced) alcohol ingestion is presented in which, by drinking, animals produce repeated, substantial elevations in blood ethanol concentration and develop physical dependence. the overindulgence is elective in that ethanol is chosen in preference to certain other fluid-ingestive alternatives. beside the usual demonstrations of acutely compromised motor performance, tolerance development, cross-tolerance, etc., the model demonstrates that the c ... | 1988 | 3067598 |
| what schedule-induced polydipsia can tell us about alcoholism. | an animal model of chronic and excessive voluntary (unforced) alcohol ingestion is presented in which, by drinking, animals produce repeated, substantial elevations in blood ethanol concentration and develop physical dependence. the overindulgence is elective in that ethanol is chosen in preference to certain other fluid-ingestive alternatives. beside the usual demonstrations of acutely compromised motor performance, tolerance development, cross-tolerance, etc., the model demonstrates that the c ... | 1988 | 3067598 |
| a case of vibrio vulnificus infection. | vibrio vulnificus, a recently described strain of the halophilic vibrio species, was isolated from the blood of a 73-year-old man, who developed rapidly progressive wound infection and fatal septicemia. twenty-one patients with vibrio vulnificus infection have been reported in the japanese literature. vibrio vulnificus most frequently causes primary septicemia and necrotising cellulitis after the eating of raw fish or shellfish or after exposure to seawater. the infection is characterized by its ... | 1988 | 3068394 |
| role of the protease in the permeability enhancement by vibrio vulnificus. | the protease produced by vibrio vulnificus enhances vascular permeability through histamine release from mast cells and activation of the plasma kallikrein-kinin system which generates bradykinin when injected into the dorsal skin. v. vulnificus living cells also enhanced vascular permeability within a few hours after the injection into the dorsal skin. the permeability-enhancing activity of living cells was greatly reduced by addition of soybean trypsin inhibitor, a specific inhibitor for plasm ... | 1988 | 3068493 |
| parasitic diarrhea. | this article reviews the biology, epidemiology, and clinical management of amebiasis, giardiasis, and cryptosporidiosis as well as the less common intestinal protozoa, dientamoeba fragilis, isospora belli, balantidium coli, and blastocystis hominis. | 1988 | 3074123 |
| parasitic diarrhea. | this article reviews the biology, epidemiology, and clinical management of amebiasis, giardiasis, and cryptosporidiosis as well as the less common intestinal protozoa, dientamoeba fragilis, isospora belli, balantidium coli, and blastocystis hominis. | 1988 | 3074123 |
| second-generation cephalosporins. | | 1986 | 3081544 |
| identification of n epsilon-carboxymethyllysine as a degradation product of fructoselysine in glycated protein. | the chemistry of maillard or browning reactions of glycated proteins was studied using the model compound, n alpha-formyl-n epsilon-fructoselysine (ffl), an analog of glycated lysine residues in protein. incubation of ffl (15 mm) at physiological ph and temperature in 0.2 m phosphate buffer resulted in formation of n epsilon-carboxymethyllysine (cml) in about 40% yield after 15 days. cml was formed by oxidative cleavage of ffl between c-2 and c-3 of the carbohydrate chain and erythronic acid (ea ... | 1986 | 3082871 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate. | lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ... | 1986 | 3085110 |
| effect of nifedipine on cerebral high-energy phosphates after cardiac arrest and resuscitation in the rat. | we studied the effect of nifedipine, a calcium entry blocker, on the recovery of cerebral adenosine triphosphate (atp), creatine phosphate (cp), and lactate levels following resuscitation from cardiac arrest. using the cardiac arrest and resuscitation model of de garavilla, babbs, and tacker with an arrest time of eight minutes, 76% of the animals arrested were resuscitated with an average intermittent abdominal compression-cpr time of 3.3 minutes. rats were assigned randomly to the following gr ... | 1986 | 3085559 |
| evidence that some membrane ligands modulate the plasmalemma fluidity of endothelial cells in culture. | the effect of some membrane ligands on the plasmalemmal fluidity of endothelial cells from bovine aorta in culture was investigated. the ligands used were: cationic ferritin (pi 8.5), soybean agglutinin, concanavalin a, wheat germ agglutinin, as well as glutaraldehyde at different concentrations. the fluidity probe employed was 1,6-diphenyl-1, 3, 5-hexatriene (dph) and the parameter determined to quantify the fluidity was fluorescence steady-state anisotropy. the optimum time interval required b ... | 1986 | 3088620 |
| nasopharyngeal carriage of neisseria meningitidis in the contacts of index cases of meningococcal meningitis. | | 1986 | 3091676 |
| [a saved case of vibrio vulnificus infection with shock, dic and necrotizing fasciitis]. | | 1986 | 3097209 |
| protamine sulfate-induced enzyme secretion from rabbit neutrophils. | protamine sulfate induces enzyme secretion from rabbit neutrophils. enzyme secretion is mainly due to exocytosis but, depending on the experimental conditions, a small amount of cytolysis may occur. as compared with stimulation of neutrophil functions by other activators, protamine sulfate-induced enzyme release by exocytosis is a relatively slow process and is not accompanied by a marked activation of the metabolic burst. for optimal exocytosis, extracellular ca2+ is required, but there is stil ... | 1986 | 3098680 |
| biosynthesis and processing of platelet gpiib-iiia in human megakaryocytes. | platelet membrane glycoprotein iib-iiia forms a calcium-dependent heterodimer and constitutes the fibrinogen receptor on stimulated platelets. gpiib is a two-chain protein containing disulfide-linked alpha and beta subunits. gpiiia is a single chain protein. these proteins are synthesized in the bone marrow by megakaryocytes, but the study of their synthesis has been hampered by the difficulty in obtaining enriched population of megakaryocytes in large numbers. to examine the biosynthesis and pr ... | 1987 | 3108266 |
| stimulation by hyphopichia burtonii and bacillus amyloliquefaciens of aflatoxin production by aspergillus flavus in irradiated maize and rice grains. | aspergillus flavus was grown on maize and rice extract agars and on irradiated viable cracked maize and rice grains, either in pure culture or in dual culture with wild strains of either hyphopichia burtonii or bacillus amyloliquefaciens. aflatoxin production by a. flavus and its growth and interactions with the other microorganisms were studied at three water activities (aw) (0.98, 0.95, and 0.90) and two temperatures (25 and 16 degrees c). both h. burtonii and b. amyloliquefaciens markedly sti ... | 1987 | 3111368 |
| comparison of glycosidase levels in bovine seminal plasma. | seven glycosidases (beta-n-acetylglucosaminidase, alpha-fucosidase, beta-galactosidase, acid alpha-glucosidase, beta-glucuronidase, acid and neutral alpha-mannosidase) were analysed in seminal plasma from the first and second successive ejaculates in normal ayrshire bulls. in comparison to our previous data the results indicate that beta-n-acetylglucosaminidase, beta-galactosidase and beta-glucuronidase are derived mainly from epididymal secretions, while alpha-fucosidase and particularly neutra ... | 1987 | 3112030 |
| inhibitory effect of capsular antigen of vibrio vulnificus on bactericidal activity of human serum. | opaque (op) and translucent (tr) colonial variants were isolated from vibrio vulnificus strains. op-type variants were more resistant than the isogenic tr-type variants, but the survival rate of the op-type variants varied with the strains. antisera were prepared by immunizing rabbit with whole cells of op and tr variants of some strains, in which the difference of the sensitivity between op and tr cells was remarkable. then agglutination tests with their living and heat-killed cells were carrie ... | 1987 | 3116370 |
| purification of an octamer sequence (atgcaaat)-binding protein from human b cells. | the highly conserved octamer sequence atgcaaat or its inverse complement found in all human and murine immunoglobulin gene promoters has been demonstrated to be necessary in the lymphoid-specific transcription by deletion analysis. trans-acting factors that interact with the octamer motif are thought to be involved in this tissue-specific expression. using a gel mobility shift assay, we have identified both lymphoid-specific and ubiquitous nuclear factors that interact with a human gamma 1 heavy ... | 1987 | 3122182 |
| [primary leiomyosarcoma of the pulmonary artery. apropos of a case]. | a case of leiomyosarcoma of the pulmonary artery in a 64-year old man without previous cardiovascular disease is reported. the clinical picture, which comprised episodes of paroxysmal dyspnoea associated with acute cor pulmonale, suggested pulmonary embolism. radioisotope perfusion study and pulmonary angiography seemed to confirm this diagnosis, but no improvement was obtained with a prolonged thrombolytic treatment. the presence of a median mass at ct led to exploratory thoracotomy and to the ... | 1987 | 3122693 |
| covalent coupling of 4-thiouridine in the initiator methionine trna to specific lysine residues in escherichia coli methionyl-trna synthetase. | a new method has been developed to couple a lysine-reactive cross-linker to the 4-thiouridine residue at position 8 in the primary structure of the escherichia coli initiator methionine trna (trnafmet). incubation of the affinity-labeling trnafmet derivative with e. coli methionyl-trna synthetase (metrs) yielded a covalent complex of the protein and nucleic acid and resulted in loss of amino acid acceptor activity of the enzyme. a stoichiometric relationship (1:1) was observed between the amount ... | 1987 | 3122828 |
| purification and partial characterization of serum monocytotropic factor, a platelet-derived cyclooxygenase-inducing polypeptide. | it has previously been shown that a heat- and acid-stable component of human and animal sera was capable of stimulating prostanoid biosynthesis in human blood monocytes, very probably by a mechanism involving cyclooxygenase induction. many physico-chemical characteristics of this factor are similar to those of identified platelet factors. here we show that human platelets are a rich source of this factor (serum monocytotropic factor) and that results from experiments using arachidonic acid or th ... | 1988 | 3124880 |
| decreased locomotor activity produced by repeated, but not single, administration of murine-recombinant interferon-gamma in mice. | a single treatment with murine-recombinant interferon-gamma (murifn-gamma; 30 micrograms/mouse), whether evaluated immediately after, or four hrs after, intraperitoneal injection, does not alter open field activity levels. on the other hand, repeated murifn-gamma administration (30 micrograms/mouse/day for 5 days) results in decreased spontaneous locomotor activity and an increase in body weight. | 1988 | 3126371 |
| adult epiglottitis due to vibrio vulnificus. | | 1988 | 3130933 |
| hiv seroprevalence in migrant and seasonal farmworkers--north carolina, 1987. | | 1988 | 3136308 |
| the biologic significance of the mixed lymphocyte kidney culture in humans. | the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ... | 1985 | 2996186 |
| examination of cardiac alpha-adrenoceptors from pharmacological responses and radioligand binding. comparison of rat and guinea pig tissues. | the object of this study was to determine suitable experimental conditions for the pharmacological evaluation of cardiac alpha-adrenoceptors. atrial and ventricular preparations of the guinea pig and rat were employed, and the alpha-adrenoceptor responsiveness was compared with the binding of the alpha-adrenoceptor radioligand [3h]prazosin in membranes prepared from these cardiac regions. the experimental variables examined were the pacing frequency, bath temperature, choice of agonist, and card ... | 1987 | 3041119 |
| effect of pyrimidinone treatment on lethal and immunosuppressive murine cytomegalovirus infection. | the 2-amino-5-halo-pyrimidinones, which are potent interferon inducers and antiviral agents, were found to be protective against lethal cytomegalovirus (cmv) challenge in weanling or neonatal mice when administered before virus challenge. this protection was dependent upon the dosage of pyrimidinone administered. weanling mice infected with a sublethal challenge of cmv exhibited moderate to severe immunosuppression as measured by reduced splenic cell blastogenic responses in vitro to the mitogen ... | 1985 | 3000290 |
| is vitellogenin an ancestor of apolipoprotein b-100 of human low-density lipoprotein and human lipoprotein lipase? | vitellogenin, an ancient animal protein, is the major yolk protein of eggs, where it is used as a food source during embryogenesis. here it is shown that vitellogenins, including those from the invertebrates caenorhabditis elegans and drosophila melanogaster, contain domains that are homologous with parts of apolipoprotein b-100 (apob-100) of human low-density lipoprotein and human lipoprotein lipase. as vitellogenins are likely to have been used by invertebrates during embryogenesis well before ... | 1988 | 3145737 |
| poor lifestyle modification among intravenous drug users assisted at a sexually transmitted disease clinic for hiv infection diagnosis, madrid, 1987. | | 1988 | 3146273 |
| isolation of the early phase of chylomicron formation in intestinal epithelial cells of rats. | lipid is first observed electron microscopically in the rough endoplasmic reticulum of intestinal epithelial cells during active lipid absorption. we have been able to isolate this subcellular fraction by using discontinuous sucrose gradients of 0.25/0.86/1.11 m sucrose. a preliminary low speed centrifugation of mucosal homogenate removed the heavier subcellular organelles. the resulting supernatant was centrifuged at 5.25 x 10(6) x g.min. the pellet from this centrifugation was placed on top of ... | 1988 | 3147719 |
| non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains. | the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ... | 1985 | 3156400 |
| structural and functional studies of the effects of sympathetic nerve stimulation on rabbit submandibular salivary glands. | continuous sympathetic stimulation at 8-10 hz caused intense vasoconstriction in the gland, so stimulation was generally given in an interrupted pattern to minimize this detrimental effect on secretion. only a small increase in fluid secretion occurred; it became thick and tended to block the cannula; therefore in later experiments the main duct was not cannulated. after sympathetic stimulation there was substantial degranulation of acinar cells. however, as this was accompanied by little moveme ... | 1988 | 3165262 |
| an interstitial deletion of chromosome 7 may characterize a subgroup of uterine leiomyoma. | | 1988 | 3165693 |
| vibrio vulnificus necrotizing fasciitis presenting with compartmental syndrome of the hand. | vibrio vulnificus necrotizing fasciitis is an unusual clinical problem. when it presented as a compartment syndrome, the picture was obscured. we report a rare case of such a presentation. a below-elbow amputation was performed and the patient recovered. | 1988 | 3171309 |
| coagglutination of vibrio cholerae, vibrio mimicus, and vibrio vulnificus with anti-flagellar monoclonal antibody. | monoclonal antibodies (mabs) with serological activity for purified flagellar (h) core protein prepared from vibrio cholerae were identified by enzyme-linked immunosorbent assay. four of these mabs reacted with the flagella of v. cholerae and v. mimicus exclusively, while eight mabs reacted with at least 1 of 30 heterologous vibrio species tested by enzyme-linked immunosorbent assay or coagglutination. it appears that v. cholerae and v. mimicus express similar, if not identical, h determinants u ... | 1988 | 3182988 |
| septicaemia caused by vibrio vulnificus. | vibrio vulnificus is an uncommon cause of septicaemia. a few reports suggest that patients with chronic liver disease are more susceptible. we report two cases of septicaemia caused by this organism and emphasise the need for early empirical therapy. | 1988 | 3183404 |
| vibrio vulnificus meningitis in a boy with thalassemia after eating raw oysters. | | 1988 | 3186359 |
| vibrio vulnificus and pulmonary infection. | | 1988 | 3195881 |
| the effect of alcoholic beverages on iron and zinc metabolism in the rat. | 1. male wistar rats (approximately 200 g) were given distilled water and a semi-synthetic control diet for 6 d. on day 7, 37 kbq 65zn were administered intramuscularly and the rats were given distilled water, beer, cider, red wine, whisky or ethanol as their only source of fluid. the wine, whisky and ethanol were diluted so that each of the beverages contained a similar ethanol concentration (approximately 30 g/l). food and fluid intake, growth rate and whole-body 65zn were measured regularly ov ... | 1988 | 3196669 |
| in vivo electrochemical detection of 5-hydroxyindole within the trigeminal nucleus caudalis of freely moving rats: the effect of morphine. | the trigeminal nucleus caudalis is considered the equivalent of the orofacial nociceptive system of the dorsal horn of the spinal cord. at the level of this trigeminal area (i.e. medullary dorsal horn) the transmission of noxious inputs is strongly modulated by a descending, serotonergic system mainly originating from the nucleus raphe magnus (nrm). the present study in freely moving animals reports the effect of morphine on the 5-hydroxyindole oxidation current recorded in the medullary dorsal ... | 1988 | 3196919 |
| vibrio vulnificus infections. | | 1988 | 3197443 |
| differential toxicity of cis and trans isomers of dichlorodiammineplatinum. | nephrotoxicity is the dose-limiting toxic effect of cis-dichlorodiammineplatinum (cis-platin) in humans. its stereoisomer transplatin does not have any toxicity at equimolar concentrations, and it also possesses little antitumor activity. in this study, subcellular localization of both the platinum isomers was examined in the liver and kidney of the mouse 24 hours following the drug administration. levels of the platinum isomers were measured using flameless atomic absorption. the results showed ... | 1988 | 3199415 |
| exploitation of bedding in dairy outdoor calf hutches by immature house and stable flies (diptera: muscidae). | | 1988 | 3204628 |
| protomyobia (acari: myobiidae) species groups and the brevisetosa complex of mites on north american shrews (mammalia: soricidae). | | 1988 | 3204630 |
| [clinical studies of rokitamycin dry syrup on skin and soft tissue infections in the pediatric fields]. | rokitamycin (rkm) dry syrup, a newly developed macrolide antibiotic, was administered to children with ages between 6 months and 15 years and 10 months suffering from skin and soft tissue infections including 41 cases of impetigo, one case of staphylococcal scalded skin syndrome (ssss) and 2 cases of subcutaneous abscess totalling 44 cases. the average daily dose level used was 31.3 mg/kg divided into 3 or 4 doses, for an average of 6 days of treatment. mics of 4 different macrolide antibiotics ... | 1988 | 3204657 |
| acute hepatic failure precipitated in a patient with subclinical liver disease by vibrionic and clostridial septicemia. | a case of fatal septicemia caused by vibrio vulnificus and clostridium bifermentans in a patient with subclinical liver disease is described. the patient appeared to recover from the infection initially after antibiotic therapy but finally succumbed to rapidly developing hepatic failure. postmortem examination revealed hemochromatosis. the pathogenesis of the polymicrobial septicemia and hepatic failure is discussed in the light of the published literature. | 1988 | 3211597 |
| in vitro activity of sulbactam/ampicillin and ampicillin against methicillin-sensitive and methicillin-resistant staphylococcus aureus and staphylococcus epidermidis. | minimum inhibitory concentrations (mics) of ampicillin and ampicillin + sulbactam (1:1) against 165 strains of staphylococcus aureus and 72 strains of staphylococcus epidermidis have been evaluated. the activity of the combination was very good. a concentration of 16 micrograms/ml + 16 micrograms/ml inhibited 96.9% of s. aureus and the 100% of s. epidermidis strains (at the same concentration ampicillin alone inhibited only 55.15% and 56.9% of s. aureus and s. epidermidis strains respectively). ... | 1988 | 3224398 |
| in vitro effect of xipamide on sodium-potassium transport systems in human erythrocytes. | the in vitro effects of xipamide in a concentration range of 10(-8) to 10(-2) m were investigated on various na+ and k+ transport systems in human red blood cells. xipamide inhibited the anion carrier or dids-sensitive lico3- -influx starting from a concentration of 10(-5) m. however, a decrease in the na+, k+-pump and the na+, k+-cotransport activity and a rise in the passive permeability of the cell membrane was only observed starting from a concentration of 10(-4) m xipamide. | 1988 | 3226221 |
| excision of cutaneous tumors in the horse using histologic guidance. | a highly successful surgical technique for removing cutaneous tumors in humans was used in seven horses with cutaneous squamous cell carcinoma (n = 3) or sarcoid (n = 4). in humans, the complete underside of the tumor is evaluated histologically by processing horizontal sections from the tumor base, and orientation between the wound surface and the undersurface of the excised tumor is maintained by mapping both surfaces. the technique ensures that small foci of residual tumor can be located accu ... | 1988 | 3227634 |
| cytolytic toxins of vibrio vulnificus and vibrio damsela. | | 1988 | 3231100 |
| [epidemiology and developmental aspects of trypanosomiasis in the source of the sangha, congo]. | | 1988 | 3233035 |
| [kidney morphology in hemolytic-uremic syndrome]. | morphology of the kidneys in hemolytic-uremic syndrome is considered basing on autopsy findings obtained for 3 infants with 5-17-day history of acute renal failure. a newborn infant of 17 days developed the disease after feto-fetal hemotransfusion when macerated fetus-donor hemolysis products entered the circulation of the fetus-recipient through monochorionic placenta. the second case in an infant of 6 months was due to adtp vaccine. the last infant aged 16 months manifested the syndrome in the ... | 1988 | 3233069 |
| [effectiveness of distal gene translation in polycistrons depends upon the arrangement of regulatory signals on a template]. | the role of the translational terminator and initiator signals arrangement for two adjacent genes in polycistronic mrna has been studied. semisynthetic beta-galactosidase gene (lacz) of e. coli and fragment of phage m13 dna (with promoter pviii, gene ix, and part of gene viii) were used for constructing of the ix-viii-lacz artificial polycistronic operon. cloning of the constructs into pbr322 vector resulted in a number of plz381n plasmids differing by the mutual arrangement of gene viii transla ... | 1988 | 3233097 |
| on the time-dependent diffusion of macromolecules through transient open junctions and their subendothelial spread. i. short-time model for cleft exit region. | in this two-part study we shall quantitatively study, using time-dependent models, the hypothesis that transient open junctions associated with widely scattered endothelial cells undergoing mitosis are the structural equivalent for the large pore pathway via which macromolecules the size of albumin or larger cross the vascular endothelium. in an earlier steady-state model [am. j. physiol. 248, h945-960 (1985)], the authors demonstrated that such an open-junction pathway could quantitatively acco ... | 1988 | 3256708 |
| antibody synthesis specific for nonoral antigens in inflamed gingiva. | in vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal b-cell activators (pba). we reasoned that if pba were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate. to test this, rabbits with experimental periodontitis were immunized in the hind legs with the histochemically detectable antigen horseradish peroxidase (hrp) or glucose oxidase ( ... | 1988 | 3257940 |
| the potency of immunoglobulin g fragments for inhibition of interference caused by anti-immunoglobulin antibodies in a monoclonal immunoradiometric assay for thyrotropin. | heterophile antibodies in patients' serum may produce false increases in apparent analyte concentrations in "sandwich"-type immunoassays. using three patients with endogenous anti-mouse igg antibodies and a two-site mouse monoclonal assay for thyrotropin, we studied the ability of igg fragments to block this positive interference. mouse whole igg and igg fc fragment blocked the interference virtually completely; igg f(ab')2 and fab fragments did not. rat and horse immunoglobulin fragments gave v ... | 1988 | 3260545 |
| syndromes of vibrio vulnificus infections. clinical and epidemiologic features in florida cases, 1981-1987. | to describe the clinical and epidemiologic features of vibrio vulnificus infections. | 1988 | 3260760 |
| anterior and posterior axial lens displacement and human aqueous outflow facility. | we studied the effect of anterior and posterior axial crystalline lens displacement (and thereby ciliary tone) on the aqueous humor outflow facility in enucleated human eyes. after attaching a reversible footplate plunger to the anterior lens capsule with cyanoacrylate, the lens was placed in one of three positions: "neutral baseline," posterior displacement (2.5 mm), or anterior displacement (2.0 mm). in seven pairs of eyes, the mean "neutral baseline" was not statistically different from the c ... | 1988 | 3262095 |
| anterior and posterior axial lens displacement and human aqueous outflow facility. | we studied the effect of anterior and posterior axial crystalline lens displacement (and thereby ciliary tone) on the aqueous humor outflow facility in enucleated human eyes. after attaching a reversible footplate plunger to the anterior lens capsule with cyanoacrylate, the lens was placed in one of three positions: "neutral baseline," posterior displacement (2.5 mm), or anterior displacement (2.0 mm). in seven pairs of eyes, the mean "neutral baseline" was not statistically different from the c ... | 1988 | 3262095 |
| light and electron microscopic studies of experimentally-induced pathologic changes in the rat periodontal tissue. | | 1988 | 3271013 |
| light and electron microscopic studies of experimentally-induced pathologic changes in the rat periodontal tissue. | | 1988 | 3271013 |
| vibrio vulnificus: did hippocrates describe a fatal case? | in the fifth century b.c., hippocrates described a fatal illness in a man who lived on an island in the aegean sea. this acute infection was characterized by black bullous skin lesions, rapidly progressive septicemia, and death on the second day. it is hypothesized that this disease was caused by vibrio vulnificus. | 1988 | 3293163 |
| vibrio vulnificus--a new monster of the deep? | | 1988 | 3293491 |
| protective immunity evoked by oral administration of attenuated aroa salmonella typhimurium expressing cloned streptococcal m protein. | attenuated strains of salmonella have been used effectively as vaccines against typhoid fever. we have investigated the use of such strains to deliver cloned antiphagocytic virulence determinants of unrelated bacteria. the aroa strain of s. typhimurium sl3261 was transformed with a low-copy plasmid vector pmk207, which contains the cloned gene spm5 encoding streptococcal m protein, the major virulence factor of these organisms. the transformed sl3261 expressed type 5 m protein in the cytoplasmic ... | 1988 | 3294331 |
| intestinal helminthiases and human health: recent advances and future needs. | | 1987 | 3294628 |
| ca2+-binding parvalbumin in rat testis. characterization, localization, and expression during development. | parvalbumin, a ca2+-binding protein, was isolated from rat testis. this is the first demonstration of the protein in endocrine glands. by using a rat parvalbumin cdna probe, parvalbumin mrna was demonstrated in the testis, indicating that the protein is synthesized in this tissue and that testis parvalbumin is a product of the same gene as the one encoding for muscle parvalbumin. parvalbumin was localized by immunohistochemical methods in the leydig cells and in the acrosome region of maturing s ... | 1987 | 3294830 |
| purification and characterization of vibrio vulnificus protease. | a protease was purified from a strain of vibrio vulnificus isolated from the blood of a septicemic human. the vibrio was cultured in bacto peptone-yeast extract medium, and the protease was purified by a purification procedure including ultrafiltration of the culture supernatant with an amicon ym 5 membrane, diethylaminoethyl-sephacel column chromatography, sephacryl s-200 column chromatography and fast protein liquid chromatography on mono q column. the protease preparation revealed homogeneity ... | 1987 | 3295490 |
| effect of posttransplantation administration of peripheral blood lymphocytes in skin-grafted mice treated with antilymphocyte serum or antilymphocyte serum plus bone marrow. | posttransplantation administration of donor-specific bone marrow cells to als-treated allograft recipients produces graft survival that is greater than that produced by als treatment alone. we have now studied the effect of peripheral blood lymphocytes (pbls) in a mouse skin allograft model using this protocol (c3h skin grafted to b6af1 mice, day 0; i.p. als on days -1 and +2; i.v. bone marrow days +6 or +7). when pbls are injected in place of bone marrow cells, graft survival is extended beyond ... | 1987 | 3299924 |
| purification and characterization of an elastolytic protease of vibrio vulnificus. | large amounts of a highly purified, extracellular elastolytic protease of vibrio vulnificus were obtained by sequential ammonium sulphate precipitation and hydrophobic interaction chromatography with phenyl-sepharose cl-4b. the protease had an mr of about 50,500 (estimated by sds-page), a pi of 5.7, and a temperature optimum range of 55 to 60 degrees c. the ph optimum and the results of inactivation studies suggested that the enzyme was a neutral metalloprotease. the protease had about 429 amino ... | 1987 | 3312481 |
| vibrio vulnificus bacteremia: report of a case and review of the literature. | | 1987 | 3316128 |
| effect of acute renal failure on insulin disposition in the isolated perfused rat kidney. | the renal disposition of insulin in acute renal failure has not been evaluated. we used the isolated perfused rat kidney to test the hypothesis that acute renal failure (arf) decreases renal insulin clearance. we used warm ischemia for 45 min, uranyl nitrate 5 mg/kg, ureter ligation, and nonfiltering kidneys as methods of inducing arf. comparisons were made with normal control kidneys. the concentrations of insulin in perfusate and urine was determined by radioimmunoassay. acute renal failure ca ... | 1987 | 3318499 |
| omental pedicle graft in the management of infected ascending aortic prostheses. | two patients had mediastinal infections with chronic draining sinus tracts that involved a vascular prosthesis in the ascending aorta. in 1 patient, a false mycotic aneurysm developed and in the other, a beginning rupture of the proximal suture line. in both patients, the infection was cured by replacing the infected aortic prosthesis combined with wrapping the new prosthesis with a pedicled omental graft. an omental graft was used to protect the vascular prosthesis and minimize the risk of recu ... | 1987 | 3318738 |
| t cell factors involved in the isotype-specific regulation of the ige antibody response. | | 1987 | 3333779 |
| ability of vibrio vulnificus to obtain iron from hemoglobin-haptoglobin complexes. | it has been suggested that the normal serum protein, haptoglobin (hp), serves a bacteriostatic role by binding free hemoglobin (hm), thus making heme iron unavailable for bacterial growth. previous studies showed that, unlike escherichia coli, vibrio vulnificus was able to overcome this hp-blocking effect. we report here a study on the iron-withholding property of the three major human hp phenotypes, hp 1, 2, and 2-1. results of experiments with human serum showed that v. vulnificus c7184 was ab ... | 1988 | 3335405 |
| alterations in aortic endothelial cell morphology and cytoskeletal protein synthesis during cyclic tensional deformation. | previous studies have shown that bovine aortic endothelial cells (ecs) in culture respond to repetitive tensional deformation with an increase in deoxyribonucleic acid synthesis and cell proliferation. this study was designed to determine whether cyclic tensional deformation of ecs in vitro induces different morphologic or protein synthetic responses. ecs from passages 6 through 9 were seeded in 35 mm2 well silicone rubber plates at 2 x 10(5) cells/well and allowed to attach for 24 hours. the ex ... | 1988 | 3336119 |
| light- and electron-microscopic analysis of the kidney in newborn rats exposed to gentamicin in utero. | kidneys of newborn rats, whose mothers were given 75 mg/kg of gentamicin daily from the tenth day of gestation to its term, were examined by light and electron microscopy, as were kidneys of control rats whose mothers were given saline during the same period. in the gentamicin-treated group, the numbers of differentiated glomeruli and of s-shaped bodies counted in a median cross-section of the kidney were reduced, compared with those in the control group, but the glomerular volume was the same. ... | 1988 | 3337212 |
| iron metabolism in the erythrophagocytosing kupffer cell. | like the peritoneal macrophage, the isolated kupffer cell is capable of processing and releasing iron acquired by phagocytosis of immunosensitized homologous red blood cells. when erythrophagocytosis is restrained to levels which do not affect cell viability, or less than 1.5 red cells/macrophage (phagocytic index of 150%), over 40% of iron acquired from red cells is released within 24 hr. more active erythrophagocytosis results in greater release of iron but progressive deterioration in cell vi ... | 1988 | 3338718 |
| expression of heme oxygenase gene in rat and human liver. | developmental changes of microsomal heme oxygenase were studied. in human fetal liver, the enzyme activity was 8 times higher than that detected in the adult liver. on the other hand, adult livers contained 4 times more cytochrome p450 than fetal livers. elevated heme oxygenase activity in fetal liver was not due to modulators present in the microsomes. similar to human, rat fetal liver also contained high enzyme activity which appeared to be regulated at the transcriptional level. hybridization ... | 1988 | 3342043 |
| hydrogen transfer by nadph-dependent reductases in elongation of very-long-chain saturated and polyunsaturated fatty-acyl-coa in swine cerebral microsomes. | the hydrogen transfer from nadph was studied in the elongation of arachidoyl-coa (20:0-coa) and arachidonoyl-coa (20:4-coa) in swine cerebral microsomes. previously, we showed that four deuterium atoms (2h) were transferred stereospecifically from (4s)-[4-2h1]nadph in the elongation of 20:0-coa to 24:0 (yoshida, s., takeshita, m. and kawaguchi, a. (1984) biochem. biophys. res. commun. 124, 322-328), and that three deuterium atoms were transferred from 2h2o in the elongation of 20:4-coa to 22:4. ... | 1988 | 3342246 |
| dna binding by 1-nitropyrene and 1,6-dinitropyrene in vitro and in vivo: effects of nitroreductase induction. | 1-nitropyrene, the predominant nitropolycyclic aromatic hydrocarbon found in diesel exhaust, is a mutagen and tumorigen. 1,6-dinitropyrene is present in diesel exhaust in much smaller quantities than 1-nitropyrene, but is much more mutagenic and carcinogenic. in an attempt to understand this difference in biological potencies, we have compared the extent of dna binding by these two nitropyrenes in vivo. we have also determined the effect of 1-nitropyrene pretreatment upon the induction of nitror ... | 1988 | 3345577 |
| electron microscope radioautographic evidence of in vivo androgen-binding protein internalization in the rat epididymis principal cells. | the androgen-binding protein (abp) has been purified from rat testes with a yield of 14% using four steps of hplc and was subsequently iodinated to a specific activity of 0.1 mci/mg protein. using a micromanipulator, [125i] iodo-abp-dihydrotestosterone was injected intraluminally into the proximal caput of the rat epididymis. epididymides were sampled from 3 to 120 min after the injection of the tracer and processed for transmission electron microscopy autoradiography. our results showed the acc ... | 1988 | 3345715 |
| early diagnosis of overwhelming vibrio vulnificus infections. | | 1988 | 3347871 |
| regional blood flows by the microsphere method: reproducibility in portal hypertensive rats and influence of a portal vein catheter. | to determine the reproducibility of splanchnic blood flow measurements by the microsphere method in rats with portal hypertension and the effects of laparotomy with portal vein cannulation, eight groups of 10 rats were studied. cardiac output and regional blood flows were measured twice, 10 min apart, in pentobarbital anesthetized or awake, sham-operated or portal vein-ligated rats, with or without portal cannulation. variability between the two successive measurements was not affected by portal ... | 1988 | 3353395 |
| purification and characterization of a family of high molecular weight surface-array proteins from campylobacter fetus. | a variety of gram-negative and gram-positive bacteria possess crystalline surface layers, although little is known of their function. we previously have shown that the high molecular weight surface-array proteins of campylobacter fetus are important in both the pathogenicity and antigenicity of this organism. for biochemical and immunological characterization, we purified high molecular weight (100,000, 127,000, 149,000) surface-array proteins from three c. fetus strains using sequential gel fil ... | 1988 | 3360785 |
| evaluation of prostaglandins as mediators of tubuloglomerular feedback. | orthograde and retrograde microperfusion experiments were conducted in sprague-dawley rats to evaluate the participation of vasoconstrictive eicosanoids as mediators of tubuloglomerular feedback (tgf) signals. retrograde perfusion with 160 microm arachidonic acid (aa) added to a hypotonic solution enhanced the stop-flow pressure (sfp) feedback responses compared with those obtained with the control hypotonic solution (delta sfp, 1.6 +/- 0.4 vs. 10.1 +/- 0.7 mmhg with aa). blockade of thromboxane ... | 1988 | 3364575 |
| high-frequency jet ventilation in a neonatal foal. | high-frequency jet ventilation was performed on a premature foal for respiratory difficulty attributable to in utero-acquired pneumonia. the procedure involves delivery of compressed gas through a small-bore cannula at frequencies up to 400 cycles/min. ventilation settings of drive pressure, frequency, and fio2 were varied to optimize pao2 and paco2 values. the foal was ventilated with this equipment for 14 hours. evidence of a favorable response to this method of ventilation was observed in the ... | 1988 | 3366679 |
| neuronal activity recorded extracellularly from in situ canine mediastinal ganglia. | spontaneous activity of 226 neurons was recorded from in situ mediastinal ganglia in 10 dogs. forty-two percent of these were active during specific phases of the cardiac cycle, primarily during systole. cardiovascular-related activity occurred when systolic pressure was between approximately 70 and 185 mmhg (1 mmhg = 133.3 pa) whether the pressure was altered by positive inotropic pharmacological agents or cross clamping of the aorta. twenty percent of the identified neurons displayed respirato ... | 1988 | 3370543 |
| occurrence of vibrionaceae in natural and cultivated oyster populations in the pacific northwest. | studies were done to assess the role of oysters from the pacific northwest as a potential source of vibrionaceae. oysters collected from natural and cultivated populations on the british columbia coast were opened using sterile instruments. the gills and oyster meat were each removed, and the meat was cut in half. the gills and the cut surface of the oyster meat were each cultured by inoculating them directly to the surface of agar plating media for the isolation of vibrionaceae. overall, 120 oy ... | 1988 | 3370928 |
| bacteremia in a veteran's administration extended care facility. | | 1988 | 3380833 |
| progesterone regulation of secretory component (sc): uterine sc response in organ culture following in vivo hormone treatment. | sex steroid hormones are known to have profound effects on mucosal immunity. in the present study we evaluated the effects of progesterone on the uterine immune system by determining the changes in the levels of secretory component (sc) released from uterine tissues in culture following in vivo administration of progesterone to estradiol-stimulated ovariectomized rats. sc is a transport protein which moves iga into external secretions such as intestinal and uterine secretions. sc release was det ... | 1988 | 3386246 |
| australian fraud inquiry under way. | | 1988 | 3386761 |