| [experimental study of hyperostosis induced by hypervitaminosis a]. | radiological and histological studies were made on retinoid-induced hyperostosis in rats. vitamin a (va) was administered intraperitoneally in rats for 6 months. hyperostosis was observed in 94 percent of rats administered va and in 38 percent of the control. chondrocytes and vascular proliferation were observed in the attachment of the tendons and in the anterior corner of the vertebral body after 3 months. hyperostosis was observed as osteophytes in the attachment of ligaments or tendons and a ... | 1992 | 1280297 |
| isolated severe thrombocytopenia and bleeding caused by piperacillin. | bleeding and severe thrombocytopenia developed in a 71-year-old man who had been receiving piperacillin 5 g intravenously every 8 h for 9 days for the treatment of pseudomonas aeruginosa septicemia. after piperacillin was discontinued, the platelet counts became normal. rechallenge was made with 2 g of piperacillin intravenously resulting in thrombocytopenia within 6 h of piperacillin administration. the platelet count normalized after 3 days when no further piperacillin was given. | 1992 | 1287819 |
| [bacteriological study of traveller's diarrhoea. 4) isolation of enteropathogenic bacteria from patients with traveller's diarrhoea at osaka airport quarantine station during 1984-1991]. | during the last 8 years (1984 to 1991), 16,639,233 overseas travellers were quarantined at osaka airport quarantine station and 38,326 travellers reported that they were (or had been) suffering from diarrhoea. bacteriological examination of stools from 12,573 persons revealed the following results. 1) various enteropathogenic bacteria were isolated from 3,669 cases (29.2%) examined. the predominant species of bacteria isolated were as follows: salmonella, 1049 cases; plesiomonas shigelloides, 10 ... | 1992 | 1293222 |
| [the lysogeny of vibrio parahaemolyticus of serovar o4:k12]. | lysogeny has been first established in strains of parahemolytic vibrios of serovar o4:k12. moderate phages belonged to morphological group iv by home a. s. tikhonenko's classification and were presented by one serological type. no correlation has been revealed between sensitivity to moderate phages of parahemolytic vibrios and specificity of "o"- or "k"-serotypes. | 1992 | 1293486 |
| features of cholera and vibrio parahaemolyticus diarrhoea endemicity in calabar, nigeria. | the clinical and epidemiological features of acute vibrio diarrhoeal disease were studied in 881 patients seen at the university of calabar teaching hospital (ucth), calabar, nigeria, between january and december 1989. stools and rectal swabs of patients and randomly-selected control subjects were microscopically and culturally examined for the presence of enteric pathogens. households of vibrio diarrhoea cases and matched controls were visited for ecologic studies. of a total of 108 (12.3%) cul ... | 1992 | 1294392 |
| a survey of urease-positive vibrio parahaemolyticus strains isolated from traveller's diarrhea, sea water and imported frozen sea foods. | the frequency of urease-positive vibrio parahaemolyticus among isolates from patients, imported frozen sea foods and the environment (sea water) was studied. the highest isolation frequency of urease-positive v. parahaemolyticus was found in clinical isolates (11.2% out of 204 strains examined). urease-positive v. parahaemolyticus was found in 5.7% of 88 frozen sea food-isolates examined, but no strains isolated from sea water were urease-positive. the isolates were further examined for the prod ... | 1992 | 1294393 |
| purification and characterization of monomeric isocitrate dehydrogenase with nadp(+)-specificity from vibrio parahaemolyticus y-4. | nadp(+)-dependent isocitrate dehydrogenase [idh: ec 1.1.1.42] was purified to electrophoretic homogeneity from vibrio parahaemolyticus y-4, and shown to be a monomeric protein of molecular weight 80,000 with a pi of 5.0. the amino acid composition and partial sequence at the n-terminus resembled those reported for other bacterial monomeric idhs. immunotitration with antisera to the monomeric and dimeric enzymes (antisera to idh-ii and -i of vibrio abe-1) showed an immunochemical distinction betw ... | 1992 | 1295895 |
| effects of phosphate in the production of hemolysin in vibrio parahaemolyticus. | vibrio parahaemolyticus is known to produce several types of hemolysin, the most document is the tdh and trh. a new type of hemolysin whose expression is repressed by the presence of phosphate in the medium is reported. | 1992 | 1298639 |
| microbiological aspects and technological need: technological needs for nitrates and nitrites. | nitrate and nitrite are used in meat and fish curing, and in the manufacture of certain cheeses. nitrate itself has little antimicrobial effect and in most applications could be replaced by lower concentrations of nitrite. further, improved hygiene diminishes the need for nitrite. the antimicrobial activity and technological needs for nitrate and nitrite are reviewed. it is concluded that the technological needs for nitrite in meat products stored at < 10 degrees c could be met by added nitrite ... | 1992 | 1298641 |
| [vestibular evoked potentials. diagnostic trends]. | the contribution of short-latency evoked potentials to the exploration of auditory, visual and somatosensory neurosensory pathways is capital. the determination of short-latency vestibular evoked potentials has encountered many difficulties, mainly due to the necessity to find a specific, very short stimulation in order to select and synchronize the vestibular nerve fibers. recent studies in animals have demonstrated short-latency potentials evoked by angular accelerations, linear accelerations ... | 1992 | 1304102 |
| expression and release of neuroregulators during development: monoamines and neuropeptides. | the turnover of catecholamines (ca) was determined in the adrenal medulla and brain of rat fetuses and pups. in general we found a considerable increase soon after birth. the expression of mrna for ca-synthesizing enzymes was also considerably enhanced in the adrenals shortly after birth. furthermore, we demonstrated increased expression of neuropeptides after birth, increased synthesis of mrna encoding for neuropeptide y in the adrenals 24 h after birth; and considerable activation of the subst ... | 1992 | 1306802 |
| brazilian urease-positive strains of vibrio parahaemolyticus carry genetic potential to produce the tdh-related hemolysin. | | 1992 | 1308550 |
| polar and lateral flagellar motors of marine vibrio are driven by different ion-motive forces. | various species of marine vibrio produce two distinct types of flagella, each adapted for a different type of motility. a single, sheathed polar flagellum is suited for swimming in liquid medium, and numerous unsheathed lateral flagella, which are produced only under viscous conditions, are suited for swarming over viscous surfaces. both types of flagella are driven by reversible motors embedded in the cytoplasmic membrane. here we report that the energy source for the polar flagellar motor of v ... | 1992 | 1309599 |
| kappa-opioid receptor stimulation abolishes mu- but not delta-mediated inhibitory control of spinal met-enkephalin release. | the possible opioid control through delta, mu and kappa receptors of the spinal release of met-enkephalin-like material (melm) was investigated in halothane-anaesthetized rats. the intrathecal perfusion of the delta agonist dtlet (10 microm) or the mu agonist dago (10 microm) resulted in a marked inhibition of melm release, which could be prevented by the selective antagonists naltrindole and naloxone, respectively. although the kappa agonist u 50488 h (10 microm) was inactive per se, it complet ... | 1992 | 1317026 |
| catabolite repression of the h(+)-translocating atpase in vibrio parahaemolyticus. | cells of vibrio parahaemolyticus grown in the presence of glucose showed reduced (by about 40%) oxidative phosphorylation. with this observation as a basis, we examined the effect of glucose on the level of h(+)-translocating atpase. the addition of glucose to the growth medium reduced the specific activity and the amount of the h(+)-translocating atpase in membrane vesicles of v. parahaemolyticus. these reductions were reversed by adding cyclic amp (camp) to the growth medium. we cloned some pa ... | 1992 | 1328162 |
| fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection. | thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ... | 1992 | 1347287 |
| [h2-receptor antagonist-refractory ulcer--its pathophysiology and role of proton-pump inhibitors]. | among h2-receptor antagonist (h2ra)-refractory ulcers, non-responders that did not heal after 5 months therapy had high intraluminal ph in the basal condition and high sensitivity to inhibition of acid secretion by h2ra but possessed gastric mucosa to generate less prostaglandins. combination therapy of pge1-analogue with h2ra healed these ulcers by 60%. proton-pump inhibitor (ppi) exerted a complete inhibition of acid secretion in these patients and the rate of healing was 88%. helicobacter pyl ... | 1992 | 1347325 |
| the role of pili in colonization of the rabbit intestine by vibrio parahaemolyticus na2. | vibrio parahaemolyticus na2 and its pili were studied in relation to intestinal colonization. the isolated pili were adhesive to the intestinal epithelium. the adhesion of the organisms was blocked by masking the epithelial receptor with the purified pili, or by treating the organisms with anti-pilus antibody (fab fraction). these results suggest that the pili of v. parahaemolyticus na2 play an important role in the adhesion of the organisms to the rabbit intestine. | 1992 | 1350048 |
| high level expression of the humanized monoclonal antibody campath-1h in chinese hamster ovary cells. | we have cloned the light and heavy chain cdnas for the humanized monoclonal antibody campath-1h and expressed them in chinese hamster ovary (cho) cells using a dihydrofolate reductase (dhfr) amplification procedure. each cdna was positioned under control of the strong human beta-actin promoter/polyadenylation signals and used to evaluate alternative vector design and amplification procedures. by employing a dual selection co-transfection strategy, initial transformants accumulated antibody level ... | 1991 | 1367214 |
| influence of low-temperature storage and glucose starvation on growth recovery in escherichia coli rela and rela+ strains. | to study the influence of microgravity on bacterial growth behavior during a space mission, the special experimental conditions and the hardware environment necessitate storage of cells at low temperature, and permit a relatively short experimental period. before this experimental period, cells have to recover their condition of steady-state growth, because it is only in this condition that the growth behavior of the flight and ground populations can be adequately compared. to meet these require ... | 1991 | 1367237 |
| eukaryotic gene regulation: simple vs complex models. | the current generally accepted model of eukaryotic gene regulation is essentially a simple one. regulatory proteins containing separable dna binding and transcriptional activation domains, bind to specific dna sequences in promotors and interact directly or indirectly with the tata box binding factor to increase the rate of transcription initiation at selected promotors. here we present observations suggesting that the process may be more complex. | 1991 | 1367248 |
| termination of second messenger signaling in olfaction. | by using isolated rat olfactory cilia and a fast kinetics methodology, it has been demonstrated that odorant-induced second messenger signaling in the millisecond time range is terminated via phosphorylation reactions catalyzed by specific protein kinases. the cyclic adenosine nucleotide pathway is turned off by kinase a activity, whereas the inositol trisphosphate cascade is terminated by kinase c. the data support the concept that desensitization of odorant responses involves phosphorylation o ... | 1992 | 1370581 |
| towards a phylogeny of the genus vibrio based on 16s rrna sequences. | the inter- and intrageneric relationships of the genus vibrio were investigated by performing a comparative analysis of the 16s rrnas of 10 species, including four pathogenic representatives. the results of immunological and 5s rrna studies were confirmed in that the genus is a neighboring taxon of the family enterobacteriaceae. with regard to the intrageneric structure, vibrio alginolyticus, vibrio campbellii, vibrio natriegens, vibrio harveyi, vibrio proteolyticus, vibrio parahaemolyticus, and ... | 1992 | 1371064 |
| electrophoretic and chemical characterization of lipopolysaccharides of vibrio parahaemolyticus. | lipopolysaccharides (lpss) isolated from three kanagawa-positive and three negative strains of vibrio parahaemolyticus were characterized by using electrophoretic, immunochemical, and chemical methods. the results of this study indicated that the lpss of all six strains of v. parahaemolyticus examined did not have an o-specific side chain. these v. parahaemolyticus lpss appeared to have molecular weights similar to that of the rough-type (ra) lps of salmonella typhimurium tv-119 and might just c ... | 1992 | 1374376 |
| lateral flagella of vibrios: serological classification and genetical similarity. | lateral (l-) flagella-having vibrios were classified into 13 h-serogroups (flagellar antigen serogroups) by means of h-agglutination test. vibrio parahaemolyticus was classified into 3 serogroups, hl1 to 3. v. alginolyticus and v. harveyi were classified into 5 and 3 serogroups, respectively, but 2 of those were serogroups common to the both species. v. fluvialis and v. furnissii constituted a same serogroup, hl8. cross-reactivity between each serogroup was not observed in h-agglutination test, ... | 1992 | 1376395 |
| disparate effects of calcium channel blockers on pressure dependence of renin secretion and flow in the isolated perfused rat kidney. | using the model of isolated perfused rat kidneys this study was performed to investigate whether or not voltage-operated calcium channels are essentially involved in the pressure control of renin secretion from the kidneys. at a perfusion pressure of 100 mm hg (13.3 kpa) renin secretory rates were 4.2 +/- 0.7 (ng angiotensin i h-1) min-1 g-1. stepwise reduction of renal perfusion pressure to 80, 60, and 40 mm hg (10.6, 8.0, 5.3 kpa) resulted in an increase of renin release yielding a 30-fold sti ... | 1992 | 1382264 |
| isolation and partial characterization of a compound with siderophore activity from vibrio parahaemolyticus. | a compound with siderophore activity was purified by successive column and thin layer chromatographic procedures from dowex 1 x 8 extracts of culture supernatants of vibrio parahaemolyticus aq 3354. the strain synthesized the compound in culture media containing less than 2 microm added fecl3. hydrolysis of the compound yielded alanine, ethanolamine, citric acid and 2-ketoglutaric acid. the 1h-nmr spectrum exhibited the presence of a residue from each of these components in the intact molecule. ... | 1992 | 1387860 |
| influence of dopaminergic and serotonergic neurons on intravenous ethanol self-administration in the rat. | rats implanted with chronic indwelling intravenous catheters and allowed access to a self-administration apparatus learned to self-inject intravenous ethanol. ethanol concentrations of 0.5, 1.0, and 2.0%, corresponding to a dose/injection of 1, 2, and 4 mg/kg, respectively, were consistently self-injected. self-injection was not acquired or maintained with ethanol doses of 0.5 or 8 mg/kg/injection. saline replacement of ethanol reservoirs led to marked increases in lever-pressing response in ani ... | 1992 | 1388276 |
| urease production in vibrio parahaemolyticus: a potential marker for virulence. | | 1992 | 1397235 |
| incidence of toxigenic vibrios in foods available in taiwan. | a total of 1088 vibrios and related species were isolated from seafood and aquacultured foods available in taiwan. they were identified as vibrio alginolyticus, v. cholerae, v. fluvialis i, v. fluvialis ii, v. parahaemolyticus, v. mimicus, aeromonas caviae, a. hydrophila, a. sobria and other species. incidence of these vibrio and aeromonas species in these foods was high. vibrio parahaemolyticus was frequently found in seawater and in foods of freshwater origin. the vibrio isolates were examined ... | 1992 | 1399913 |
| vibrio-associated gastroenteritis in the lower cross-river basin of nigeria. | a total of 120 vibrio species were isolated from 588 patients with acute diarrheal disease during an outbreak of gastrointestinal tract infections at different locations in the lower cross river basin of nigeria. vibrio cholerae o1, biotype el tor, serotype ogawa, was the prominent organism isolated from the vibrio-associated diarrheal cases. during the 3 months of study, v. cholerae non-o1 was recovered from 10 patients while vibrio parahaemolyticus was recovered from 19 patients. the significa ... | 1992 | 1400976 |
| [in vitro antimicrobial activity of dr-3355, a new quinolone antibacterial agent, against clinical isolates of enteritis-causing bacteria]. | we determined the minimum inhibitory concentration (mic) of dr-3355, a newly developed quinolone-derivative antibacterial agent, against clinical isolates of various bacterial species from enteritis patients, and compared them with those of ofloxacin (oflx), ciprofloxacin (cpfx), nalidixic acid (na), ampicillin (abpc), kanamycin (km). mic90 of dr-3355 against 94 strains of shigella spp. and 5 strains of escherichia coli, 36 strains of salmonella spp., 22 strains of vibrio cholerae, 5 strains of ... | 1992 | 1402063 |
| [methods to detect the thermostable direct hemolysin gene and a related hemolysin gene of vibrio parahaemolyticus by pcr]. | | 1992 | 1404920 |
| characterization of a vibrio cholerae virulence factor homologous to the family of tonb-dependent proteins. | irga is an iron-regulated virulence factor for infection in an animal model with classical vibrio cholerae strain 0395. we detected gene sequences hybridizing to irga at high stringency in clinical isolates in addition to 0395, including another classical strain of v. cholerae, three v. cholerae strains of the el tor biotype, three non-o1 isolates of v. cholerae, and individual isolates of vibrio parahaemolyticus, vibrio fluvialis, and vibrio alginolyticus. no hybridization to irga was seen with ... | 1992 | 1406279 |
| chemotactic activity of hemocytes derived from a brackish-water clam, corbicula japonica, to vibrio parahaemolyticus and escherichia coli strains. | hemocytes from adult and juvenile specimens of a brackish-water clam, corbicula japonica, were attracted chemotactically to live cells of vibrio parahaemolyticus and escherichia coli strains in a balanced salt solution, which was enhanced significantly in the presence of respective c. japonica plasma. chemotactic attractions of adult's and juvenile's hemocytes were seen also in artificial seawater at a similar level to those in the balanced salt solution. chemotactic attractions of juvenile's he ... | 1992 | 1420564 |
| vibrio parahaemolyticus septicemia in a patient with neutropenic leukemia. | | 1992 | 1420695 |
| the regulation of microsomal glucose-6-phosphatase differs between liver and kidney. | | 1992 | 1426558 |
| activation of t cells in vivo using anti-cd3 and staphylococcal enterotoxins. | one of the major problems in clinical immunity is that neoplastic and virally infected cells are insufficiently immunogenic to trigger an immune response. during the past several years, our laboratory has explored the use of t-cell-specific reagents including monoclonal antibodies (mabs) and staphylococcal enterotoxins to amplify immune responses. this report summarizes our efforts to augment immunity with these agents. | 1992 | 1428401 |
| thermostable, salt tolerant, wide ph range novel chitobiase from vibrio parahemolyticus: isolation, characterization, molecular cloning, and expression. | a chitobiase gene from vibrio parahemolyticus was cloned into plasmid puc18 in escherichia coli strain dh5 alpha. the plasmid construct, pc120, contained a 6.4 kb vibrio dna insert. the recombinant gene expressed chitobiase [ec 3.2.1.30] activity similar to that found in the native vibrio. the enzyme was purified by ion exchange, hydroxylapatite and gel permeation chromatographies, and exhibited an apparent molecular weight of 80 kda on sds-polyacrylamide gel electrophoresis. chitobiose and 6 mo ... | 1992 | 1429506 |
| modification of the disc assay by the incorporation of monoclonal antibody staining. | the use of immunohistochemical staining with the disc assay is described. this modified staining procedure allows differentiation of viable human tumor cells from normal cells in biopsy preparations, facilitating the evaluation of slides from chemosensitivity assays. samples from all and cll patients were processed using both the disc and modified moab-disc procedure. the predictive results were compared and found to be identical. this procedure is advantageous in determining the chemosensitivit ... | 1992 | 1434746 |
| use of an oligonucleotide probe to detect vibrio parahaemolyticus in artificially contaminated oysters. | a 26-mer oligonucleotide specific to vibrio parahaemolyticus was synthesized from a 1,275-bp thermostable direct hemolysin (tdh) gene. this oligonucleotide probe specifically reacted with dna from 89 of 95 v. parahaemolyticus isolates but not with dna from other vibrios or other enteric and nonenteric organisms (n = 48). the probe hybridized with southern blots of 0.5-kb hindiii-restricted chromosomal dna fragments from all but five v. parahaemolyticus test isolates. the probe could be used to d ... | 1992 | 1444377 |
| [the comparative characteristics of vibrio cholerae hemolysins]. | biochemical and biological properties of in vivo and in vitro hemolysins of cholera germs isolated by different authors as well as hemolysins of vibrio parahaemolyticus and escherichia coli have been comparatively characterized. according to the above data hemolysin of cholera germ el tor is a thermolabile protein with molecular mass of 60-80 thou.; it is cytotoxic, enteropathogenic and lyses all the species of erythrocytes. hemolysins of v. eltor and germs are immunologically related. hemolysin ... | 1992 | 1453988 |
| identification of amino acid changes affecting yeast uroporphyrinogen decarboxylase activity by sequence analysis of hem12 mutant alleles. | the molecular basis of the uroporphyrinogen decarboxylase defect in eleven yeast 'uroporphyric' mutants was investigated. uroporphyrinogen decarboxylase, an enzyme of the haem-biosynthetic pathway, catalyses the decarboxylation of uroporphyrinogen to coproporphyrinogen and is encoded by the hem12 gene in the yeast saccharomyces cerevisiae. the mutations were identified by sequencing the mutant hem12 alleles amplified in vitro from genomic dna extracted from the mutant strains. four mutations lea ... | 1992 | 1471989 |
| transforming growth factor beta and basic fibroblast growth factor synergistically promote early bovine embryo development during the fourth cell cycle. | developmentally competent bovine blastocysts were produced by adding transforming growth factor beta (tgf beta) and basic fibroblast growth factor (bfgf) to serum-free cultures of in vitro produced, 2-cell bovine embryos. the effects of tgf beta were evaluated because this growth factor signals synthesis and secretion of the extracellular matrix component fibronectin and its receptor. previous investigations have demonstrated that fibronectin promotes early bovine embryo development in vitro. th ... | 1992 | 1472373 |
| purification and characterization of kanagawa haemolysin from vibrio parahaemolyticus. | the haemolysin of a kanagawa-phenomenon-positive vibrio parahaemolyticus strain was purified to apparent homogeneity by acid precipitation, deae-trisacryl, hydroxyapatite and fplc (mono-q) columns: 1.4 micrograms of protein gave a single band on conventional sds-page with silver staining. the haemolysin was not inactivated by heating for 10 min at 100 degrees c. it was a monomeric protein with a molecular weight estimated to be 29 kda by page under denaturing and non-denaturing conditions. the h ... | 1992 | 1475518 |
| the thermostable direct hemolysin of vibrio parahaemolyticus is a pore-forming toxin. | the hemolytic mechanism of thermostable direct hemolysin (tdh), a possible virulence factor of vibrio parahaemolyticus, was studied. we demonstrated that tdh acts as a "pore-forming toxin" in temperature-dependent and -independent steps. the first temperature-dependent step requires only about 1-2 min incubation at 37 degrees c and makes a "pore" with a functional diameter of approximately 2 nm. the pore size was deduced from the molecular diameter of the colloidal inhibitory polysaccharides. th ... | 1992 | 1477791 |
| ultrastructural alterations of brain cortex in rat following intraperitoneal administration of mercuric chloride. | intraperitoneal administration of a single dose (6 mg/kg body weight) of mercuric chloride (hgcl2) results in ultrastructural changes in brain cortex of the rats. 18 hours after administration of hgcl2 the accumulation of dense deposits of mercury in nerve and glial cell cytoplasm was observed. the quantity of microglia in neuropil was also increased in this experimental group. we postulate that these cerebral macrophages can play an important role in the process of intoxication, too. 5 days aft ... | 1992 | 1479187 |
| detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of vibrio parahaemolyticus by polymerase chain reaction. | polymerase chain reaction (pcr) protocols were established for specific detection of the tdh and trh genes, the virulence marker genes of vibrio parahaemolyticus encoding two related hemolysins. the tdh and trh genes are known to have sequence divergence of up to 3.3% and 16%, respectively. attempts were made to find suitable primer pairs and annealing temperatures to detect each gene without fail. dnas extracted from 36 representative strains of v. parahaemolyticus were used in the initial scre ... | 1992 | 1480187 |
| non-isotopic microtitre plate-based assay for detecting products of polymerase chain reaction amplification: application to detection of the tdh gene of vibrio parahaemolyticus. | a non-isotopic microtitre plate-based assay method was devised for detection of products of the polymerase chain reaction. this assay involves affinity immobilization of the biotinylated amplification products in microtitre plate wells and their fluorescence detection by their hybridization with an oligonucleotide probe linked to alkaline phosphatase. an advantage of this procedure is that the immobilization and hybridization are carried out simultaneously in the wells, thus shortening the assay ... | 1992 | 1480188 |
| [the phages of halophilic vibrios and their use]. | the range of the lytic activity of 46 phages of parahemolytic vibrios isolated from lysogenic strains, sea water samples, crabs and mussels has been studied. the phages are represented by virions belonging to morphological groups ii, iv, v according to the phage classification currently used in russia and to different serological groups. no relationship between the sensitivity of vibrio strains to the phages under study and the specificity of serotypes o and k has been established. the preparati ... | 1992 | 1481605 |
| professional doc (pdoc): a software environment for authoring and consulting of electronic documentation. interactive diagnosis and therapy: a medical application. | professional doc (pdoc) is a software environment for electronic documentation authoring and consulting based on the hypermedia technology. it allows to manage different kinds of information (pictures, sounds, texts, graphics, video) organized according to hyperstructures. the user-friendliness and effectiveness of its man-machine interface, and its availability on the most common hardware platforms makes it a suitable product for a very broad category of users. interactive diagnosis and therapy ... | 1992 | 1483002 |
| [the suicide phenomenon and fermentative metabolic activity in strains of the aeromonas group isolated from feces]. | we study the "suicide" phenomena as well as metabolic pathways of mixed acids (methyl red test, mr) and butylene glycol (voges-proskauer, vp), in 107 strains belonging to mesophilic aeromonas group, isolated from stools. the strains have been identified as a. hydrophila, 28 cases (26.1%), a. sobria 26 cases (24.3%) and a. caviae 53 cases (49.6%). all a. caviae strains behave homogeneously as f+, rm+ and vp-, independently of temperature (30 or 37 degrees c). a. hydrophila strains only showed the ... | 1992 | 1489794 |
| susceptibility of the brine shrimp artemia and its pathogen vibrio parahaemolyticus to chlorine dioxide in contaminated sea-water. | adults and nauplii of the brine shrimp, artemia, together with vibrio parahaemolyticus, were placed in sewage-contaminated sea-water which had been treated with chlorine dioxide (hallox e-100tm) to test its potential as a disinfectant for salt water aquaculture. the nauplii were very susceptible to low concentrations of chlorine dioxide (47 micrograms/l cl-), but the adults were slightly more resistant. sterile sea-water treated with lower concentrations of chlorine dioxide (less than 47 microgr ... | 1992 | 1490907 |
| enterotoxigenicity of vibrio parahaemolyticus with and without genes encoding thermostable direct hemolysin. | vibrio parahaemolyticus produces a thermostable direct hemolysin (tdh) that has been implicated in the pathogenesis of diarrheal disease caused by this organism. however, previous studies attempting to demonstrate the contribution of the hemolysin to virulence have been inconclusive. we investigated this putative virulence factor by using an isogenic tdh-negative (tdh-) strain constructed by specifically inactivating the two copies of the tdh gene encoding tdh. the enterotoxigenicities of the pa ... | 1992 | 1500161 |
| behavioral analysis of vibrio parahaemolyticus variants in high- and low-viscosity microenvironments by use of digital image processing. | digital image analysis and light microscopy were used to study and quantify the growth and behavior of two variants and selected flagellar mutants of vibrio parahaemolyticus in glass flow cells under high- and low-viscosity conditions. the observations showed a series of surface-associated behaviors, including attachment, microcolony formation, migration, chemotactic movements, and aggregation, indicating a substantial degree of adaptive flexibility and multicellular behavior during growth of v. ... | 1992 | 1512206 |
| sequence variation in the thermostable direct hemolysin-related hemolysin (trh) gene of vibrio parahaemolyticus. | our previous molecular epidemiologic study with gene probes (h. shirai, h. ito, t. hirayama, y. nakamoto, n. nakabayashi, k. kumagai, y. takeda, and m. nishibuchi, infect. immun. 58:3568-3573, 1990) demonstrated that the gene (trh) encoding a thermostable direct hemolysin-related hemolysin was strongly associated with clinical strains of vibrio parahaemolyticus. strain-to-strain variation in the intensities of the hybridization signals observed in the above study also suggested that the trh gene ... | 1992 | 1514791 |
| development of the immunomagnetic enrichment method selective for vibrio parahaemolyticus serotype k and its application to food poisoning study. | a method using immunomagnetic separation was developed to isolate the specified k serotype of vibrio parahaemolyticus from a mixture of a large number of bacteria with other k serotypes. this method was applied to food poisoning studies and could recover the v. parahaemolyticus serotype found in the patient from the incriminated foods. | 1992 | 1514817 |
| [sensitivity of vibrio and aeromonas to antibiotics]. | the antibiotic sensitivity of 696 cultures belonging to the family vibrionaceae (v. cholerae o1, v. cholerae non-o1, v. albensis, v. parahaemolyticus, v. alginolyticus and aeromonas spp.) was studied and general regularities of the antibiotic sensitivity were shown: a high sensitivity to broad-spectrum antibiotics (tetracycline and chloramphenicol) and a low sensitivity to ++beta lactams (carbenicillin and ampicillin). the comparative examinations revealed similarity in the antibioticograms of v ... | 1992 | 1514858 |
| evidence for the existence of a restriction-modification system common to several species of the family vibrionaceae. | a broad-host-range vibriophage kvp40 originally isolated on vibrio parahaemolyticus 1010 was restricted and modified by strains of at least five vibrio and one photobacterium species. 1010 was a non-restricting host. an anti-restriction mutant kvp40 aar1 was isolated after propagating the phage on a restricting host, v. anguillarum vib36. kvp40 aar1 grown on either 1010 or vib36, as well as the parental phage grown on vib36, showed much higher efficiencies of plating on all the restricting hosts ... | 1992 | 1521769 |
| effects of iron limitation on production of a siderophore, outer membrane proteins, and hemolysin and on hydrophobicity, cell adherence, and lethality for mice of vibrio parahaemolyticus. | vibrio parahaemolyticus is one of the most important enteropathogens in taiwan, japan, and other coastal regions. the pathogenesis of v. parahaemolyticus disease is not clearly understood. the expression of some factors by v. parahaemolyticus in iron-rich and iron-limited media was analyzed. in the clinical hemolytic strains, the production of a siderophore, two outer membrane proteins (77 and 80 kda), and thermostable direct hemolysin was significantly enhanced in iron-limited culture, and hemo ... | 1992 | 1535342 |
| risk factors for suicide attempts in a human immunodeficiency virus screening program. | | 1992 | 1539099 |
| cloning and expression of a cytosolic megakaryocyte protein-tyrosine-phosphatase with sequence homology to retinaldehyde-binding protein and yeast sec14p. | protein tyrosine phosphorylation is important in the regulation of cell growth, the cell cycle, and malignant transformation. we have cloned a cdna that encodes a cytosolic protein-tyrosine-phosphatase (ptpase), meg2, from meg-01 cell and human umbilical vein endothelial cell cdna libraries. the 4-kilobase cdna sequence of ptpase meg2 corresponds in length to the mrna transcript detected by northern blotting. the predicted open reading frame encodes a 68-kda protein composed of 593 amino acids a ... | 1992 | 1557404 |
| detection and quantitative assessment of a vibrio cholerae o1 species in several foods by a novel enzyme immunoassay. | a selected antibody enzyme immunoassay (saeia) for the general detection of vibrio cholerae o1 species has been developed using the immunological reagents of a rabbit antiserum specific for v. cholerae o1 classical inaba 569b and immobilized cell fragments of v. cholerae o1 el tor 85p6, and beta-d-galactosidase-labeled goat anti-rabbit immunoglobulin g as tracer. the saeia was specific for v. cholerae o1 species and showed low cross-reaction values to other microorganism species tested including ... | 1992 | 1584068 |
| a broad-host-range vibriophage, kvp40, isolated from sea water. | a broad-host-range vibriophage, kvp40, was isolated from sea water by using vibrio parahaemolyticus 1010 (eb101) as the indicator host. the host range of kvp40 extended over at least 8 vibrio and 1 photobacterium species. kvp40 was a large tailed phage containing double-stranded dna and belonged to ackermann's morphotype a2. kvp40 dna was cleaved by 11 different type ii restriction endonucleases including ecori and hindiii, but not by 17 other enzymes including bamhi, kpni and sali. | 1992 | 1584076 |
| the highest gene concentrations in the human genome are in telomeric bands of metaphase chromosomes. | chromosome in situ suppression hybridization has been carried out on human metaphase chromosomes to localize the g+c-richest human dna fraction (which only represents 3.5% of the genome), as isolated by preparative equilibrium centrifugation in cs2so4/3,6-bis(acetatomercurimethyl)-1,4-dioxane density gradient. this fraction essentially corresponds to isochore family h3. the rationale for carrying out this experiment is that this isochore family has, by far, the highest gene concentration, the hi ... | 1992 | 1594593 |
| haemagglutinating and buccal epithelial cell adherence activities of vibrio parahaemolyticus: correlation with virulence. | clinical and environmental isolates of vibrio parahaemolyticus were examined for their ability to agglutinate human and rabbit erythrocyte and to adhere to human buccal epithelial (hbe) cells in the presence or absence of mannose. all strains produced cell-associated haemagglutinins (hgs) after 3 h at 37 degrees c. mannose-sensitive haemagglutination (msha) appeared to be a significant marker for differentiating between clinical and environmental isolates; 75% of clinical and 11% of environmenta ... | 1992 | 1597877 |
| comparison of vibrio parahaemolyticus hemolysin (vp-trh) produced by environmental and clinical isolates. | tdh-related hemolysin (vp-trh) produced by kanagawa-phenomenon-negative (kp-) vibrio parahaemolyticus has been demonstrated to be a possible virulence determinant. though almost half of kp- isolates examined from diarrhoeal patients produced vp-trh, few reports mentioned the ability of environmental isolates to produce vp-trh. considering the route of infection with v. parahaemolyticus, this toxin must be produced by the organisms in the sea or in sea food. to confirm that vp-trh produced by v. ... | 1992 | 1601286 |
| chemotactic activity of hemocytes derived from two marine neritid gastropod molluscs, nerita albicilla and heminerita japonica, to vibrio parahaemolyticus and escherichia coli strains. | hemocytes of two marine neritid gastropods, nerita albicilla and heminerita japonica, were attracted chemotactically to live vibrio parahaemolyticus and escherichia coli strains. chemotactic attraction of n. albicilla hemocytes was enhanced in the presence of n. albicilla plasma, while that of h. japonica hemocytes was not enhanced in the presence of h. japonica plasma. chemotactic activity of the hemocytes seems to participate in the rapid elimination of v. parahaemolyticus from these gastropod ... | 1992 | 1606253 |
| examination by site-directed mutagenesis of the amino acid residues of the thermostable direct hemolysin of vibrio parahaemolyticus required for its hemolytic activity. | the amino acid sequences of eight variants of thermostable direct hemolysin (tdh) and a tdh-related hemolysin, deduced from nucleotide sequences, were compared. fourteen amino acid residues, mainly within conserved regions, were chosen to prepare mutant tdhs with one or two amino-acid replacements by site-directed mutagenesis. of the 25 mutant tdhs prepared, those with replacements of trp65 (trp65-his65, trp65-tyr65, trp65-leu65) or leu66 (leu66-ser66) showed very significant reduction (more tha ... | 1992 | 1630298 |
| low ph deforms the influenza virus envelope. | influenza virus membrane fusion is induced by low ph, which triggers an irreversible conformational change in the viral haemagglutinin (ha). the result of this change is the extrusion of the ha fusion peptide, after which it may act in the fusion of virus and endosomal membranes. here we describe electron microscopic observations on low ph-treated virus after negative staining or cryo-electron microscopy of virus in the frozen hydrated state. the results indicate a destabilization of the virus m ... | 1992 | 1634881 |
| [hairy cell leukemia successfully treated with 2'-deoxycoformycin following refractoriness to splenectomy and alpha-interferon therapy]. | a 34-year-old man was admitted in may, 1989 because of severe left upper abdominal pain, which was caused by advanced splenomegaly. on initial examinations, peripheral blood showed leukocytosis (13,400/ml) including 60% hairy cell which also infiltrated in bone marrow (64%). the patient was diagnosed as having hairy cell leukemia (japanese type) of b-cell lineage. splenectomy was performed as an initial treatment. the effect of splenectomy was only palliative and transient leukocytosis progresse ... | 1992 | 1635172 |
| total hepatic mesh wrap for hemostasis. | to improve hemostasis and avoid the complications of perihepatic packing, a technique of hepatic tamponade using total mesh wrap is reported. the method is geometrically, technically and mechanically feasible and appears to be effective in controlling severe parenchymatous bleeding. | 1992 | 1636144 |
| enzyme-labeled oligonucleotide probes for detection of the genes for thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) of vibrio parahaemolyticus. | alkaline phosphatase conjugated oligonucleotide probes were developed to detect the genes (tdh and trh) coding for the thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) of vibrio parahaemolyticus. using dot blot hybridization, probes were tested with 94 clinical isolates of v. parahaemolyticus. results agreed well with those obtained using radio-labeled recombinant dna probes for the genes tdh and trh. specificity and sensitivity of enzyme tdh probes for detection of the trh ge ... | 1992 | 1643584 |
| isolation and characterization of new restriction endonucleases from vibrio parahaemolyticus: vpak32i enzyme with the class-iis heptanucleotide specificity, gctcttcn1/n4. | six restriction endonucleases (enases), classified into four different specificities, were found in a screen among 68 reference strains of vibrio parahaemolyticus of human origin. five of these enases are isoschizomers of well-known enases, while the remaining one, designated vpak32i, is a novel and highly efficient class-iis enase with the hepatanucleotide recognition site, 5'-gctcttc(1/4)-3'. | 1992 | 1644299 |
| evidence for insertion sequence-mediated spread of the thermostable direct hemolysin gene among vibrio species. | the tdh gene of vibrio parahaemolyticus which encodes the thermostable direct hemolysin has been found in some strains of other vibrio species. analysis of seven tdh genes cloned from v. parahaemolyticus, vibrio mimicus, and non-o1 vibrio cholerae revealed that all tdh genes were flanked by insertion sequence-like elements (collectively named isvs) or related sequences derived from genetic rearrangement of isvs. the isvs possessed 18-bp terminal inverted repeats highly homologous to those of is9 ... | 1991 | 1650342 |
| obligatory role of ca2+ in the cytotoxic activity of dengue virus-induced cytotoxin. | the role of calcium ions (ca2+) in the cytotoxic activity of the dengue type 2 virus (dv)-induced macrophage (m phi) cytotoxin (cf2) was investigated in the present study. the findings show that cf2 prepared in ca(2+)-free medium had no cytotoxic activity on normal mouse spleen cells suspended in ca(2+)-free medium but killed the cells suspended in the medium with ca2+. substitution with calcium chloride restored the cytotoxic activity of cf2 the optimal dose being 10(-4) m concentration. cf2 in ... | 1991 | 1653589 |
| chemical structure of the carbohydrate backbone of vibrio parahaemolyticus serotype 012 lipopolysaccharide. | the chemical structure of the saccharide portion of vibrio parahaemolyticus serotype 012 lipopolysaccharide was studied. using chemical degradation and modification, as well as methylation analysis in combination with glc-ms, laser-desorption mass spectrometry and 1h-nmr and 13c-nmr spectroscopy, the carbohydrate backbone of the lipopolysaccharide was characterized as a branched decasaccharide with the following structure: (formula; see text) in the native lipopolysaccharide two additional phosp ... | 1991 | 1655425 |
| [isolation of enteropathogenic microorganism from patients with infection of the digestive tract during 1976 to 1988 in tenri hospital]. | enteropathogenic microorganisms isolated from feces of 9,393 patients with diarrhea or enteritis in our hospital between 1976 and 1988 were analyzed. as the result of the examination of 5,443 outpatients, 1,811 strains of pathogens were isolated from 1,686 cases (31.0%). several species including salmonella spp., escherichia coli serotype, vibrio parahaemolyticus, were isolated before 1978, and the incidence of pathogens was low (14.8%). for the 10-year period since 1979, the incidence markedly ... | 1991 | 1655921 |
| regulation of the mouse mammary tumor virus (mmtv) binding site in cultured mammary tissue. | the mouse mammary tumor virus (mmtv) initiates infection when the envelope protein, gp52, binds to a cell surface protein and triggers internalization. the hormonal regulation of this protein was studied both in vitro and in vivo. in mammary gland explant culture, levels of the mmtv binding protein were maintained only when prolactin was present in the medium. the further addition of progesterone induced levels to 164% of controls, while estradiol was without effect. these results were reproduce ... | 1991 | 1665450 |
| purification and characterization of pili isolated from vibrio parahaemolyticus na2. | pili from vibrio parahaemolyticus na2 isolated from a patient with diarrhea were purified and characterized. the organisms were hemagglutinative, but the purified pili were not. na2 pili were physicochemically and immunologically quite different from the previously described v. parahaemolyticus ha7 pili. nevertheless, there was a high degree of homology between their n-terminal amino acid sequences. | 1991 | 1670933 |
| [a method of isolating vibrio parahaemolyticus from different substrates]. | a method for isolation of parahemolytic vibrios is suggested, based on their high mobility in semiliquid agar gel. a special semiliquid medium was designed and tried, containing agar-agar, peptone, sodium chloride, mannose, gelatin, indicator, progress agent. the material is inoculated into the center of a petri dish. after incubation at 37 degrees c parahemolytic vibrios form macrocolonies on the surface of the medium, colored black against the red background of the medium. this method permits ... | 1990 | 1695981 |
| a simple polymerase chain reaction method for detection and cloning of low-abundance transcripts. | a simplified polymerase chain reaction (pcr) technique for the detection, semiquantitation and cloning of low-abundance rnas is described. this assay involves first-strand cdna synthesis by reverse transcription of mrna with specific oligonucleotide primers, followed by second-strand synthesis and pcr amplification, in the same tube with only the addition of taqi dna polymerase. the assay is sufficiently sensitive to detect target rna from as little as 1 ng of total rna. the beta-actin transcrip ... | 1990 | 1698071 |
| heterogeneity of distribution pattern at the electron microscopic level of heparan sulfate in various basement membranes. | using the high-iron diamine thiocarbohydrazide silver proteinate (hid-tch-sp) staining technique and enzymatic digestion, we investigated the ultrastructural distribution pattern of heparan sulfate side chains of heparan sulfate proteoglycan (hspg) in various basement membranes (nerve, capillary, oral epithelial, muscle, and dental basement membranes). four different distribution patterns of stain deposits were identified as heparan sulfate on the basis of enzymatic degradation by heparitinase. ... | 1990 | 1698204 |
| comparison of immunochemical specificities of vibrio parahaemolyticus o10 and o12 antigens using monoclonal antibodies. | the monoclonal antibodies against vibrio parahaemolyticus o10 and o12 antigens (lipopolysaccharide, lps) were prepared and specificities of the antibodies were examined. five of six anti-o10 antibodies reacted with o10 antigen, but none of them reacted with o12 antigen. on the contrary all of the five anti-o12 antibodies reacted with o10 antigen as well as homologous o12 antigen. o10 and o12 antigens were subjected to alkali treatment or periodate oxidation, and reactivities of these chemically ... | 1991 | 1725552 |
| [advances in the research of clinical use of recombinant human granulocyte colony-stimulating factors]. | | 1991 | 1726145 |
| analysis of high psa n-cam expression during mammalian spinal cord and peripheral nervous system development. | using a monoclonal antibody that recognizes specifically a high polysialylated form of n-cam (high psa n-cam), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (drgs) extracts microdissected at different developmental stages. analysis of the ratio of high psa n-cam to total n-cam indicated that sialylation and desia ... | 1991 | 1769342 |
| adherence of vibrio parahaemolyticus to rabbit intestinal epithelial cells in vitro. | kanagawa positive strains of vibrio parahaemolyticus showed adherence whereas most of the kanagawa negative strains were non-adhering to rabbit intestinal epithelial cells. anti-hemolysin antisera did not inhibit the adherence of v. parahaemolyticus strains. moreover, the adhesive capacity of non-adhering strains was not enhanced by purified hemolysin. cell surface hydrophobicity remained the same in both kanagawa positive and negative strains of v. parahaemolyticus. fetuin strongly inhibited th ... | 1991 | 1769547 |
| sequence and features of the tryptophan operon of vibrio parahemolyticus. | the nucleotide sequence of the trp operon of the marine enteric bacterium vibrio parahemolyticus is presented. the gene order e, g, d, c(f), b, a is identical to that of other enterics. the structural genes of the operon are preceded by a long leader region encoding a 41-residue peptide containing five tryptophan residues. the organization of the leader region suggests that transcription of the operon is subject to attenuation control. the promoter-operator region of the v. parahemolyticus trp o ... | 1991 | 1773058 |
| [effects of sodium chloride on destruction of microorganisms by microwave heating in potatoes]. | to assess the destructive effect of different sodium chloride concentrations (0, 0.3, 1.5, and 3%) on microorganisms with microwave heating, strains of vibrio parahaemolyticus, staphylococcus aureus, salmonella enteritidis, escherichia coli and bacillus cereus were used to inoculate a mashed potato preparation. after microwave heating for 1 min at 800 w, resulted in temperatures and rate of destruction of bacteria significantly higher (p less than 0.05) in the core than on the surface of mashed ... | 1991 | 1773068 |
| mosaic trisomy 8 associated with jejunal duplication. | | 1991 | 1773546 |
| [work capacity of man when using individual respiratory protective devices]. | gas-mask decreased the ability to work by 20-50%. it was caused by decreasing skin and respiratory evaporation. ventilation of the under mask interspace (90 1/min) was recommended. | 1991 | 1790865 |
| purification and characterization of a lecithin-dependent haemolysin from escherichia coli transformed by a vibrio parahaemolyticus gene. | lecithin-dependent haemolysin (ldh) of vibrio parahaemolyticus was purified from escherichia coli c600 transformed with a plasmid (phl591) ligated with a 1.5 kb dna fragment of v. parahaemolyticus. the final preparation comprised two ldh proteins with different molecular masses which were immunologically cross-reactive and had the same enzymic activity. the ldh was a phospholipase hydrolysing both fatty acid esters of phospholipid, i.e. it hydrolysed phosphatidylcholine (pc) to lysophosphatidylc ... | 1991 | 1791426 |
| synthesis, antimicrobial, and antitumor activity of a series of palladium(ii) mixed ligand complexes. | mixed ligand complexes of cisdichloromethioninepalladium(ii) with 2-mercaptopyrimidine and 2-aminopyrimidine were synthesized and characterized by elemental analysis, conductivity data, infrared, and 1h nmr and 13c nmr spectra. in these mixed ligand complexes methionine coordinates to palladium through amino nitrogen and sulphur, thus leaving a free carboxylic acid group. the pyrimidine ligand coordinates to metal ion through n3. mixed ligand complexes of cisdichloroethioninepalladium(ii) with c ... | 1991 | 1791468 |
| contribution of the tdh1 gene of kanagawa phenomenon-positive vibrio parahaemolyticus to production of extracellular thermostable direct hemolysin. | kanagawa phenomenon-positive strains of vibrio parahaemolyticus contain two copies of the tdh gene (tdh 1 and tdh 2) encoding thermostable direct hemolysin (tdh). previous studies suggested that the tdh 2 gene, but not the tdh 1 gene, was responsible for production of extracellular tdh. in this study, a tdh 2-deficient isogenic mutant of kanagawa phenomenon-positive strain aq3815 was constructed by a suicide vector-mediated in vivo recombination method. the intact tdh 1 gene in the mutant contri ... | 1991 | 1795634 |
| unconjugated and conjugated bilirubin pigments during perinatal development. iii. studies on serum of breast-fed and formula-fed neonates. | the process of conjugation and secretion of bilirubin was studied in a group of healthy, full-term, exclusively breast-fed newborns and a control group of exclusively formula-fed infants by means of a reverse-phase high-performance liquid chromatographic analysis of bilirubins present in serum. the serum concentrations of unconjugated bilirubin, esterified bilirubin, and the proportion of diesterified bilirubin (as percent of esterified bilirubin) were not significantly different in breast- and ... | 1991 | 1797114 |
| unconjugated and conjugated bilirubin pigments during perinatal development. iii. studies on serum of breast-fed and formula-fed neonates. | the process of conjugation and secretion of bilirubin was studied in a group of healthy, full-term, exclusively breast-fed newborns and a control group of exclusively formula-fed infants by means of a reverse-phase high-performance liquid chromatographic analysis of bilirubins present in serum. the serum concentrations of unconjugated bilirubin, esterified bilirubin, and the proportion of diesterified bilirubin (as percent of esterified bilirubin) were not significantly different in breast- and ... | 1991 | 1797114 |
| comparison of the electrophysiological effects of org 7797, disopyramide, mexiletine and propafenone in anaesthetized dogs with myocardial infarcts. | 1. the electrophysiological effects of intravenously administered org 7797 were compared with those of disopyramide (class ia), mexiletine (ib) and propafenone (ic) in anaesthetized dogs with 5-6 day-old left ventricular myocardial infarcts. 2. org 7797 (0.5 mg kg-1) slowed conduction at all levels of the myocardium as shown by increases in st-a, ah, hv and qrs intervals, very modestly prolonged atrial and ventricular refractory periods and slightly shortened ventricular repolarization. sinus no ... | 1991 | 1797309 |
| comparison of the electrophysiological effects of org 7797, disopyramide, mexiletine and propafenone in anaesthetized dogs with myocardial infarcts. | 1. the electrophysiological effects of intravenously administered org 7797 were compared with those of disopyramide (class ia), mexiletine (ib) and propafenone (ic) in anaesthetized dogs with 5-6 day-old left ventricular myocardial infarcts. 2. org 7797 (0.5 mg kg-1) slowed conduction at all levels of the myocardium as shown by increases in st-a, ah, hv and qrs intervals, very modestly prolonged atrial and ventricular refractory periods and slightly shortened ventricular repolarization. sinus no ... | 1991 | 1797309 |
| purification and some properties of inducible lysine decarboxylase from vibrio parahaemolyticus. | inducible lysine decarboxylase from vibrio parahaemolyticus aq 3627 was purified to apparent homogeneity and characterized. the enzyme displayed a molecular weight of 531000 by gel filtration and 79000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the enzyme required pyridoxal phosphate as a cofactor, and the ph optimum was 5.5. the km value for l-lysine was 3.2 mm, and the enzyme was inhibited by 6-aminocaproic acid and alpha-fluoromethyl analogs of cadaverine. delta-hydroxylysi ... | 1991 | 1799949 |
| purification and characterization of a hemolysin of vibrio mimicus that relates to the thermostable direct hemolysin of vibrio parahaemolyticus. | a hemolysin (designated vm-rtdh) from vibrio mimicus (aq0915-e13) was purified by ammonium sulfate fractionation and successive column chromatography with deae-sephadex a-25, hydroxyapatite, mono q, superose 12 and phenyl-superose. the mr of the subunit was estimated to be about 22,000 by sodium dodecyl sulfate-slab gel electrophoresis. the isoelectric point of vm-rtdh was approximately ph 4.9. the hemolytic activity of vm-rtdh was stable upon heating at 100 degrees c for 10 min, similar to that ... | 1991 | 1804756 |