| biotin transport in microvillous membrane vesicles, cultured trophoblasts, and isolated perfused human placenta. | biotin, essential for normal fetal growth and development, must be transported across the placenta to reach the fetus. this study evaluated placental transport of biotin using microvillous membrane vesicles (mmv), cultured trophoblasts, and isolated perfused cotyledon. biotin uptake in mmv was stimulated by an inward na+ gradient. in the presence of na+, maximal stimulation was observed with cl-, among various anions. biotin uptake required 1 na+ per biotin molecule. kinetic analysis in mmv show ... | 1992 | 1539622 |
| effects of cardioplegic solutions and their components on human saphenous vein contractility. | cardioplegic solution administration into the vein graft is an established method to ensure cardioplegic distribution beyond coronary artery stenoses. the ultrastructural demonstration of severe endothelial damage after cardioplegic exposure suggests that intravenous cardioplegic administration can contribute to early and late graft thrombosis. the direct effect on human saphenous vein contractility of three cardioplegic solutions and their components was compared. a solution with 30 mmol/l k+ a ... | 1992 | 1540064 |
| heavy-ion effects on bacteria spores: the impact parameter dependence of the inactivation. | the impact parameter dependence of the inactivation of bacillus subtilis-spores has been measured using a heavy-ion minibeam facility, which permits single-ion exposure of individual spores with a spatial accuracy of about 1 micron. the apparatus consists basically of a collimator and a microscope used to position the biological objects directly behind the collimator. measurements were obtained for nickel, tin, and uranium ions at 1.4 mev/u. for central hits the results show an inactivation prob ... | 1992 | 1542713 |
| lcrf is the temperature-regulated activator of the yada gene of yersinia enterocolitica and yersinia pseudotuberculosis. | the virulence plasmid of human pathogenic yersinia species, pyv, encodes secreted proteins, yop proteins, and an outer membrane protein, yada. yada has been associated with binding to a variety of substrates and with interference with host defense. yada is regulated by temperature and is expressed only at 37 degrees c. unlike the yop regulon, the yada gene is not under ca2+ regulation. here, we show that lcrf (virf), the temperature-regulated activator of the yop regulon, also acts as an activat ... | 1992 | 1548243 |
| molecular cloning, iron-regulation and mutagenesis of the irp2 gene encoding hmwp2, a protein specific for the highly pathogenic yersinia. | under iron-starvation, the highly pathogenic yersinia synthesize several iron-regulated proteins including two high-molecular-weight polypeptides (hmwp1 and hmwp2). from the chromosome of yersinia enterocolitica serovar o:8 (strain ye 8081), the genes coding for the hmwp2 (irp2) and its promoter were cloned into plasmid puc18 (pir2) and used as a probe. we show here that the irp2 gene is present only in the highly pathogenic strains and that its promoter is iron-regulated in escherichia coli. af ... | 1992 | 1552851 |
| cloning and characterization of the eae gene of enterohaemorrhagic escherichia coli o157:h7. | the eae (escherichia coli attaching and effacing) gene from enteropathogenic escherichia coli (epec) was previously shown to be essential for production of the 'attaching and effacing' histopathology characteristic of epec infections (jerse et al., 1990). we have now cloned the eae gene from enterohaemorrhagic e. coli (ehec) which, in addition to producing shiga-like cytotoxins, also produces the attaching and effacing effect. the sequence homology between the epec and ehec sequences was 86% and ... | 1992 | 1552854 |
| new clues to how bacteria get into cells. | | 1992 | 1553529 |
| [molecular and cellular bases of the virulence of shigella flexneri]. | shigella flexneri, a gram negative bacillus, causes bacillary dysentery, an ulcerative disease of the human colon, by invading intestinal epithelial cells. entry into epithelial cells occurs via an induced phagocytic process which involves the actino-myosin complex. the host-cell receptor and the transmembrane signal which initiate reorganization of the cytoskeleton are under study. binding to integrins has recently been demonstrated in related models such as the entry of yersinia pseudotubercul ... | 1992 | 1554236 |
| mechanistic studies of the biosynthesis of 3,6-dideoxyhexoses in yersinia pseudotuberculosis. purification and stereochemical analysis of cdp-d-glucose oxidoreductase. | an nad(+)-dependent cdp-d-glucose oxidoreductase which catalyzes the first step of the biosynthesis of cdp-ascarylose (cdp-3,6-dideoxy-l-arabino-hexose), converting cdp-d-glucose to cdp-4-keto-6-deoxy-d-glucose, was isolated from yersinia pseudotuberculosis. a protocol consisting of deae-cellulose, matrex blue-a, hydroxylapatite, deae-sephadex, sephadex g-100, and nad(+)-agarose column chromatography was used to purify this enzyme 6000-fold to homogeneity. this enzyme consists of two identical s ... | 1992 | 1556102 |
| induction of primary antiphospholipid syndrome in mice by immunization with a human monoclonal anticardiolipin antibody (h-3). | antiphospholipid syndrome (apls) is characterized by thrombocytopenia, thromboembolic phenomena, and recurrent fetal loss, associated with anticardiolipin antibodies (aca) and/or lupus anticoagulant. the syndrome may be primary or may be associated with other conditions such as systemic lupus erythematosus. we have previously shown the ability to induce apls in naive mice following passive transfer of serum and monoclonal acas. similarly we generated the secondary apls in balb/c mice following i ... | 1992 | 1569194 |
| cloning and nucleotide sequence of the eae gene homologue from enterohemorrhagic escherichia coli serotype o157:h7. | the eae gene has recently been shown to be necessary for the attaching and effacing (ae) activity of enteropathogenic escherichia coli (epec) on intestinal epithelial cells. in this paper we report the cloning and nucleotide sequence of a similar gene from a strain of enterohemorrhagic e. coli (ehec) serotype o157:h7. an ehec eae sequence was identified which was 97% homologous to the epec eae gene for the first 2200 bp and 59% homologous over the last 800 bp. both eae sequences show 50% homolog ... | 1992 | 1577255 |
| a novel protein, lcrq, involved in the low-calcium response of yersinia pseudotuberculosis shows extensive homology to yoph. | the plasmid-encoded yop genes of pathogenic yersiniae are regulated by the environmental stimuli calcium and temperature. a novel protein, lcrq, which exhibits a key function in the negative calcium-controlled pathway, was identified. dna sequence analysis revealed that lcrq has a molecular mass of 12,412 daltons and its isoelectric point is 6.51. overexpression of lcrq in trans in wild-type yersinia pseudotuberculosis ypiii(pib102) changed the phenotype from calcium dependence to calcium indepe ... | 1992 | 1577700 |
| tyrosine protein kinase inhibitors block invasin-promoted bacterial uptake by epithelial cells. | the ability to enter into (invade) mammalian cells is an essential virulence determinant of many pathogenic bacteria and intracellular parasites. these organisms are internalized by host cells upon attachment to their surface. however, the mechanisms used by intracellular parasites to induce internalization into host cells have not been defined. we found that the protein kinase inhibitor staurosporine blocks invasion by some pathogenic bacteria, including yersinia enterocolitica and yersinia pse ... | 1992 | 1587588 |
| [the ionized calcium in the blood of domestic chickens: dependence on age and sex]. | in the present study concentration of ionized calcium and the correlation between total and ionized calcium in blood of domestic fowl and its dependence on age and sex were investigated. furthermore a possible influence of blood sampling procedure on the measured values was assessed. leghorn chickens of either sex, aged 3, 9, 25, and 80 weeks, respectively, were used. blood sampling was performed by vein-puncture and indwelling catheters, respectively, of both ulnaris vein and artery of animals ... | 1992 | 1590033 |
| behavioral heterogeneity in sprague-dawley rats. | because of consistent individual differences in animal behavior, it is often difficult to interpret experimental data. we therefore attempted to rank sprague dawley rats by a hierarchical ascendant clustering technique using data from avoidance conditioning and open-field testing (scores of exploratory activity before, during, and after an unpredictable noise stress, and calculated stress-related inhibition). this procedure allowed partition of the animal population into distinctive groups and s ... | 1992 | 1594674 |
| [mono-arthritis of uncertain etiology--a follow-up study]. | 78 patients in whom the cause of a monoarticular arthritis remained unclear after an initial workup were contacted for a follow-up interview (and an additional clinical examination in 64 cases) after 6 to 11 years (mean 8 years). the mean age at the onset of symptoms was 39 years, with 51% of the patients presenting in the range between 20 and 40 years. there was a slight male preponderance (56%). large joints, mainly the knee, and less often the wrist, ankle or hip, were affected in 79%. finger ... | 1992 | 1594908 |
| involvement of membrane carbohydrates of hela cells in the e. coli hb101 (pri203) invasive pathway. | the recombinant plasmid pri203 carries a yersinia pseudotuberculosis chromosomal gene that makes e. coli k-12 hb101 strain able to synthetize an outer membrane protein, invasin, which interacts with integrin receptors of eukaryotic cells, enabling this microorganism to penetrate human cultured animal cells. in this study we evaluated the involvement of hela cell membrane structural components in the early phases of the invasive pathway of e. coli hb101 (pri203). when hela cell monolayers were tr ... | 1992 | 1602981 |
| antibodies against yersinia among farmers and slaughterhouse workers. | antibodies to immunoglobulins (ig) m, g, and a against yersinia enterocolitica serotypes o:3, o:5, o:8, and o:9 and yersinia pseudotuberculosis serotypes i and iii were analyzed by enzyme immunoassay of the serum samples of 161 slaughterhouse workers, 147 pig farmers, and 114 grain or berry farmers. the crude risk ratios for elevated serum antibody concentrations were calculated with the use of the grain and berry farmers as the reference population. the risk for an elevated y enterocolitica o:3 ... | 1992 | 1604274 |
| [experimental paratuberculosis in sheep after intravenous or oral inoculation: pathogenicity and biologic diagnosis]. | an experimental paratuberculosis study was performed in sheep. one group of 6 lambs was inoculated intravenously with the equivalent of 50 mg (wet weight) of live bacilli, another group of 6 lambs was inoculated orally by placing 500 mg (wet weight) of live organisms in milk feed and a group of 3 lambs was used as controls. the degree of cellular immunity was followed by examining delayed hypersensitivity using 3 allergens (bovine tuberculin ppd, avian tuberculin ppd and johnine ppd) and that of ... | 1992 | 1610074 |
| cytometric haematological analysis of experimental yersinia pseudotuberculosis infection at temperatures of cultivation of 25 degrees c and 37 degrees c. | the dynamics of acute lethal infection with yersinia pseudotuberculosis (serovar iii) grown at 25 degrees c and 37 degrees c, respectively, were studied in guinea-pigs. using an automated hematology analyzer, the quantity and quality of the host cellular response was determined at the primary site of infection (the peritoneal cavity) and in parallel in the blood. a much more intensive leucocytic response of monocytic type was observed in the animals infected with microorganism grown at 37 degree ... | 1992 | 1611207 |
| the difference in the lcrv sequences between y. pestis and y. pseudotuberculosis and its application for characterization of y. pseudotuberculosis strains. | we have sequenced the lcrgvh operon from y. pseudotuberculosis plasmid pyv995 and compared its sequence with that of y. pestis. the sequences were highly homological, however, six base pair substitutions were found in one short 14 bp region termed variable sequence. two oligonucleotides corresponding to variable sequence of y. pestis (pes-v) or y. pseudotuberculosis (ptb-v) were synthesized and were used as molecular probes in hybridization experiments with sets of y. pestis and y. pseudotubercu ... | 1992 | 1614327 |
| [the human chorionic gonadotropin (hcg) receptor: a new class within the family of gtp protein coupled receptors. epitope mapping of receptor-bound agonistic and antagonistic forms of hcg]. | human chorionic gonadotropin (hcg) is a member of the glycoprotein hormone family. it is composed of an alpha and a beta subunit, the latter being closely homologous to that of human luteinizing hormone (hlh). hcg and hlh bind to the same receptor. the molecular mass of hcg is 38 kd, up to 30% of which is contributed by the carbohydrate moieties linked to each of the two subunits. structure-function relationship studies have indicated that both subunits interact with the receptor and that altoge ... | 1992 | 1636261 |
| a spontaneous yersinia pseudotuberculosis-infection in a monkey-colony. | a spontaneous yersinia pseudotuberculosis (y.p.) infection in one african green monkey (cercopithecus aethiops), and nine squirrel monkeys (saimiri sciureus), is reported. the clinical findings, laboratory results and pathological findings are presented. the digestive system was the most affected organ in the acute phase of the y.p. infection, while in the subacute and chronic phases, the alterations of the lymphatic tissues, spleen or liver were severe. | 1992 | 1642076 |
| epizootic of yersinia pseudotuberculosis in a wildlife park. | an epizootic attributable to yersinia pseudotuberculosis infection was confirmed in captive ruminants in a wildlife park by microbiologic and histologic findings. an epornitic of y pseudotuberculosis infection was identified at the same period in a vicinity near the ruminant deaths. yersinia pseudotuberculosis can infect human beings and cause acute enteritis and mesenteric lymphadenitis. people in contact with infected animals should use extreme caution and use good sanitary precautions to prec ... | 1992 | 1644638 |
| prevalence of antibodies to five selected zoonosis agents in monkeys. | the prevalence of antibodies against 5 zoonosis agents was determined in serum samples of 443 breeding monkeys. of the monkeys, 296 were bred or kept for a long time at r institute, and the remaining 147 were newly imported from the philippines and kept at s institute for quarantine. antibodies to simian virus 40 were highly prevalent at 89.1% among monkeys of r institute, whereas no antibody could be detected in those of s institute. antibodies to chlamydia psittaci and yersinia pseudotuberculo ... | 1991 | 1657210 |
| pulse field electrophoresis of the chromosome of the pathogenic yersiniae. | | 1991 | 1657518 |
| [group disease in children caused by enteroviruses and yersinia]. | | 1991 | 1663297 |
| proliferation of chicken lymphoblastoid cells after in vitro infection with marek's disease virus. | activated, thymus-derived (t) lymphoblasts were exposed to marek's disease virus and cultivated in attempts to induce in vitro transformation. after 9 to 15 days, colonies or small clusters of proliferating lymphoblasts were observed in cultures from three of a total of 122 attempts. these developed into proliferating cell cultures that resembled conventional marek's disease (md) lymphoblastoid cell lines in terms of growth characteristics and morphology. all proliferative cultures were unusual ... | 1991 | 1664718 |
| [phenotypic features of a strain of yersinia pseudotuberculosis with the pvm82 plasmid, detected at pseudotuberculosis outbreak foci]. | the phenotypic properties conferred to yersinia pseudotuberculosis cells by the genetical determinants of a 25md fragment of the plasmid pvm82 coding for the modified cellular immune response in the infected organism. the fragment was shown to determine the conjugative properties of the plasmid, the resistance of bacterial cells to a number of hydrophobic agents and cellular ability to absorb the congo red dye. the latter confirms the presence of additional structural components in the cell wall ... | 1991 | 1664911 |
| correlation between cd4 cell counts and cellular and plasma viral load in hiv-1-seropositive individuals. | we conducted a study of 152 hiv-1-seropositive individuals in order to evaluate the possible correlations between the isolation of hiv from peripheral blood mononuclear cells or from plasma and cd4 cell counts. hiv was isolated from only 36% of plasma samples, and the isolation rate was closely related to cd4 cell counts, increasing gradually from 0% in subjects with greater than 800 x 10(6)/l cd4 cells to 88% in those with less than 100 x 10(6)/l cd4 cells. in contrast, hiv was isolated from 92 ... | 1991 | 1676276 |
| identification of the integrin binding domain of the yersinia pseudotuberculosis invasin protein. | the invasin protein of the pathogenic yersinia pseudotuberculosis mediates entry of the bacterium into cultured mammalian cells by binding several beta 1 chain integrins. in this study, we identified the region of invasin responsible for cell recognition. thirty-two monoclonal antibodies directed against invasin were isolated, and of those, six blocked cell attachment to invasin. these six antibodies recognized epitopes within the last 192 amino acids of invasin. deletion mutants of invasin and ... | 1990 | 1693333 |
| the yersinia enterocolitica inv gene product is an outer membrane protein that shares epitopes with yersinia pseudotuberculosis invasin. | an essential virulence attribute for yersinia enterocolitica and yersinia pseudotuberculosis is the ability to invade the intestinal epithelium of mammals. the chromosomal invasin gene (inv) has been cloned from both of these yersinia species, and the y. pseudotuberculosis invasin has been well characterized (r. r. isberg, d. l. voorhis, and s. falkow, cell 50:769-778, 1987). here we constructed tnphoa translational fusions to the y. enterocolitica inv gene to identify, characterize, and localiz ... | 1990 | 1694524 |
| thrombospondin deposition in rat carotid artery injury. | the balloon catheter injury model was used to determine the relative contributions of vascular smooth muscle cells (smc) and platelets to thrombospondin (tsp) antigen deposition in the artery wall. rat carotid arteries were denuded of endothelium, exposing the thrombogenic subendothelial extracellular matrix (ecm) to the circulation. rats were killed after 1 hour, or 5, 10, or 20 days. thrombospondin antigen deposition in the injured arteries was assessed using a specific polyclonal antiserum ra ... | 1990 | 1695483 |
| effects of substance p on [ca2+]i and force in intact guinea pig ileal smooth muscle. | in intact sheets of the guinea pig ileal longitudinal muscle, loaded with fura-2, both substance p (sp)- and k(+)-induced contractile responses are preceded by cytoplasmic free ca2+ ([ca2+]i) variations. in response to sp (10(-10)-10(-7) m), [ca2+]i and force increased concentration dependently. from 10(-9) m on, the response was biphasic: an initial ca2+ spike and force transient were followed by a tonic component. the [ca2+]i and force vs. log [sp] curves were sigmoidal for the initial phasic ... | 1990 | 1695485 |
| dna recombination and natural selection pressure sustain genetic sequence diversity of the feline mhc class i genes. | sequence comparisons of seven distinct mhc class i cdna clones revealed that feline class i molecules have a remarkable similarity to human hla genes in their organization of functional domains as well as in the nonrandom partitioning of genetic variability according to the functional constraints ascribed to different regions of the mhc molecule. the distribution of the pattern of sequence polymorphism in the cat as compared with genetic diversity of human and mouse class i genes provides eviden ... | 1990 | 1695669 |
| the postsynaptic 43k protein clusters muscle nicotinic acetylcholine receptors in xenopus oocytes. | nicotinic acetylcholine receptors (achrs) are localized at high concentrations in the postsynaptic membrane of the neuromuscular junction. a peripheral membrane protein of mr 43,000 (43k protein) is closely associated with achrs and has been proposed to anchor receptors at postsynaptic sites. we have used the xenopus oocyte expression system to test the idea that the 43k protein clusters achrs. mouse muscle achrs expressed in oocytes after injection of rna encoding receptor subunits are uniforml ... | 1990 | 1698395 |
| the postsynaptic 43k protein clusters muscle nicotinic acetylcholine receptors in xenopus oocytes. | nicotinic acetylcholine receptors (achrs) are localized at high concentrations in the postsynaptic membrane of the neuromuscular junction. a peripheral membrane protein of mr 43,000 (43k protein) is closely associated with achrs and has been proposed to anchor receptors at postsynaptic sites. we have used the xenopus oocyte expression system to test the idea that the 43k protein clusters achrs. mouse muscle achrs expressed in oocytes after injection of rna encoding receptor subunits are uniforml ... | 1990 | 1698395 |
| projection subtypes of rat neostriatal matrix cells revealed by intracellular injection of biocytin. | individual neostriatal-matrix spiny neurons were stained intracellularly with biocytin after intracellular recording in vivo, and their axons were traced into the globus pallidus (gp), entopeduncular nucleus (ep), and/or substantia nigra (sn). the locations of the neurons within the matrix compartment of the neostriatum (ns) were established by immunocytochemical counterstaining of sections containing the cell bodies using antibodies for calbindin-d28k. this allowed nearly complete visualization ... | 1990 | 1698947 |
| [quantitative determination of the carbohydrate determinants of o-antigens of yersinia pseudotuberculosis and pseudomonas fluorescens]. | interactions of o-specific polysaccharides, lps and lps-protein complex with specific antibodies isolated on a polysaccharide-substituted immunoadsorbent gel and monovalent fab-fragments of the antibodies were investigated. the association constants and quantity of fab-fragments bound to these antigens were established. the interaction of the lps and lps-protein complexes was shown to depend considerably on concentration of antigens, and only 10-15% of carbohydrate determinants are accessible fo ... | 1990 | 1704703 |
| tyrosine phosphate hydrolysis of host proteins by an essential yersinia virulence determinant. | the plasmid-encoded yoph protein is a protein-tyrosine phosphatase (ptpase; ec 3.1.3.48) that is essential for yersinia virulence. we have investigated the molecular basis for the role of ptpase activity in yersinia pathogenesis. allelic recombination was employed to introduce a defined mutation into the yoph plasmid gene. conversion of the essential cys-403 to ala in the catalytic domain of the protein abolished yoph ptpase activity and significantly reduced the virulence of yersinia pseudotube ... | 1991 | 1705028 |
| analysis of the v antigen lcrgvh-yopbd operon of yersinia pseudotuberculosis: evidence for a regulatory role of lcrh and lcrv. | virulent yersinia species possess a common plasmid that encodes essential virulence determinants (yops) which are regulated by the extracellular stimuli ca2+ and temperature. the v antigen operon was recently shown to be involved in the ca2(+)-regulated negative pathway (a. forsberg and h. wolf-watz, mol. microbiol. 2:121-133, 1988). we show here that the v antigen-containing operon of yersinia pseudotuberculosis is a polycistronic operon having the gene order lcrgvh-yopbd. dna sequencing analys ... | 1991 | 1705541 |
| an o antigen can interfere with the function of the yersinia pseudotuberculosis invasin protein. | escherichia coli strains harbouring the yersinia pseudotuberculosis inv gene are able to enter cultured mammalial cells. we show here that this property is not shared by all enteric bacteria, since shigella flexneri 2a cured of its virulence-associated plasmid and harbouring the inv gene is unable to enter mammalian cells efficiently. mapping studies showed that the region of the chromosome responsible for this phenotype includes rfab, a locus involved in the production of o antigen. s. flexneri ... | 1991 | 1710312 |
| [the use of growth stimulants in the bacteriologic diagnosis of yersinia]. | the effects of k. s. karpuzidi's stimulants (0.5-1 percent) and of para-amino benzoic acid (0.1-05 mm) on the growth and multiplication of 3 yersinia pseudotuberculosis, 1 y. enterocolitica strains, and e. coli nonpathogenic strain were examined. karpuzidi's stimulant was found to selectively stimulate yersinia cell growth and inhibit escherichia development in conditions unfavorable for these bacteria. para-amino benzoic acid intensively stimulated the growth of both yersinia and escherichia. w ... | 1991 | 1710732 |
| eric sequences: a novel family of repetitive elements in the genomes of escherichia coli, salmonella typhimurium and other enterobacteria. | we describe a family of highly conserved, enterobacterial repetitive intergenic consensus (eric) sequences, 14 of which have been identified in escherichia coli and salmonella typhimurium and a further three in other enterobacterial species (yersinia pseudotuberculosis, klebsiella pneumoniae and vibrio cholerae). eric sequences are 126 bp long and appear to be restricted to transcribed regions of the genome, either in intergenic regions of polycistronic operons or in untranslated regions upstrea ... | 1991 | 1713281 |
| a ribosomal protein of yersinia pseudotuberculosis having partial epitope identity with hla-b27. | the phenotype hla-b27 is common in patients who develop reactive arthritis after having an infection. one hypothesis concerning the pathogenesis of reactive arthritis is that molecular mimicry between hla-b27 and certain bacterial components might be involved. it is known that an infection with yersinia is commonly associated with reactive arthritis in b27 positive patients. therefore, we were interested to investigate whether cross-reactivity between yersinia and hla-b27 exists. a gene library ... | 1990 | 1715196 |
| mapping and topographic localization of epitopes of the yersinia pseudotuberculosis invasin protein. | the yersinia pseudotuberculosis invasin protein is a 986-amino-acid outer membrane protein that promotes bacterial penetration into mammalian cells by binding to beta 1-chain integrin receptors. we previously showed that the integrin binding domain is encoded by the carboxyl-terminal 192 amino acids. to further investigate the structure of this protein, we characterized a set of 32 monoclonal antibodies (mabs) directed against invasin. invasin deletion derivatives and fusion proteins carrying di ... | 1991 | 1716609 |
| an extended antigenic scheme for yersinia pseudotuberculosis. | | 1991 | 1718658 |
| molecular cloning and genetic characterization of the rfb region from yersinia pseudotuberculosis serogroup iia, which determines the formation of the 3,6-dideoxyhexose abequose. | the rfb region of yersinia pseudotuberculosis serogroup iia has been cloned and expression of o antigen in escherichia coli k12 was demonstrated. transposon mutagenesis analysis confined the dna region required for o antigen expression to a 19.3 kb fragment, and the o antigen expressed was visualized by sds-page and silver staining. southern hybridization analysis demonstrated significant levels of similarity between the yersinia rfb region and the 3,6-dideoxyhexose pathway genes rfbf and rfbg, ... | 1991 | 1724263 |
| human platelet aggregation by yersinia pseudotuberculosis is mediated by invasin. | plasmid-free strains of yersinia pseudotuberculosis induce aggregation of human platelets in vitro. it appears that this phenomenon is mediated by invasin (inv), a 103-kda outer membrane protein that permits bacteria to penetrate mammalian cells, since (i) an isogenic inv-deficient mutant failed to aggregate platelets compared with the parental strain; (ii) a monoclonal antibody directed against invasin inhibited platelet aggregation; (iii) inv+ escherichia coli hb101 promoted platelet aggregati ... | 1992 | 1730468 |
| binding of cultured mammalian cells to immobilized bacteria. | the invasin protein of yersinia pseudotuberculosis binds to integrin receptors on mammalian cells and promotes cellular penetration. we demonstrate here that the cell attachment activity of invasin can be detected in bacterial colonies that have been immobilized on filter membranes. invasin expressed in either escherichia coli k-12 or y. pseudotuberculosis mediated binding to membranes, and as few as 10(5) y. pseudotuberculosis resulted in detectable attachment of cultured epithelial cells. a si ... | 1992 | 1730504 |
| isolation of yersinia enterocolitica serovar o8 from free-living small rodents in japan. | yersinia species were isolated from 65 of 223 free-living small mammals trapped in 10 regions on honshu island in japan. of the 65 strains isolated, 1 was yersinia enterocolitica serovar o3, 8 were y. enterocolitica o5, 6 were y. enterocolitica o8, 3 were y. enterocolitica o9, and 1 was yersinia pseudotuberculosis 4b. of the six y. enterocolitica o8 strains, five were positive for autoagglutination, ca2+ dependence, and the 45-mda virulence plasmid and showed high pathogenicity for mice. | 1992 | 1734061 |
| [molecular and cellular bases of shigella flexneri virulence]. | shigella flexneri, a gram negative bacillus, causes bacillary dysentery, an ulcerative disease of the human colon, by invading intestinal epithelial cells. entry into epithelial cells occurs via an induced phagocytic process which involves the actino-myosin complex. the host-cell receptor and the transmembrane signal which initiate reorganization of the cytoskeleton are under study. binding to integrins has recently been demonstrated in related models such as the entry of yersinia pseudotubercul ... | 1991 | 1742620 |
| susceptibility of wild mice to yersinia pseudotuberculosis and yersinia enterocolitica. | laboratory-born progeny and adult populations of old world woods mice (apodemus speciosus) trapped in a certain area where yersinia pseudotuberculosis was prevalent among wild animals, were challenged with y. pseudotuberculosis and yersinia enterocolitica to determine the ecology of yersinia in wild mice. the laboratory-born progeny population was associated with infection with y. pseudotuberculosis but not with y. enterocolitica serotype o:3 and was found to be nearly 200 times more susceptible ... | 1991 | 1755925 |
| the nucleotide and deduced amino acid sequence of the cationic 19 kda outer membrane protein omph of yersinia pseudotuberculosis. | the omph proteins of enteric bacteria are recently described, small (16 kda), cationic outer membrane proteins. because a yersinia pseudotuberculosis cell envelope protein of this size has been found to cross-react serologically with the human histocompatibility antigen hla-b27 (b*2701), the sequence of y. pseudotuberculosis omph was determined by sequencing the gene region which encodes mature omph. a protein consisting of 143 amino acid residues was found. it was 96% homologous with the omph o ... | 1991 | 1756172 |
| yersiniosis in free-ranging muskoxen on banks island, northwest territories, canada. | sixty-seven muskox (ovibos moschatus) carcasses, 53 skeletal remains and two sick muskoxen were seen during an aerial survey of the thomsen river region, northern banks island, northwest territories, canada in late july 1986. complete necropsies were performed on 29 muskoxen estimated to have died within the previous 3 to 5 days. twenty were diagnosed with acute yersiniosis due to yersinia pseudotuberculosis serotype 1b and were in excellent body condition. a diagnosis could not be made on seven ... | 1991 | 1758017 |
| [an experimental study of yersinia in plants]. | y. enterocolitica and y. pseudotuberculosis have been experimentally found to penetrate into plants (cabbage, salad, pea, oat) from infected soil and water and to remain in different plant organs (roots, seeds, leaves) up to 30 days (the term of observation). the processes of s-r dissociation in yersinia cultures isolated from plants, as well as changes in some enzymatic properties, have been established. in the test on infusoria the cytotoxic action of strains isolated from plants has been note ... | 1991 | 1759521 |
| afterloading increases the left ventricular end-systolic force-length relation slope in dogs. | we evaluated the effects of pressure loading produced by gradual aortic occlusion on left ventricular (lv) myocardial contractility by assessing changes in the slope of the lv end-systolic force-diameter (fes-des) relation. eleven adult mongrel dogs were prepared with ultrasonic crystals for measuring lv diameter and a micromanometer for measuring lv pressure. preload was decreased by vena caval occlusion (vco), and afterload was increased by aortic occlusion (aoo). the slopes (ec) and extrapola ... | 1991 | 1774832 |
| yersinia in effluents from the food-processing industry. | yersinia enterocolitica and yersinia pseudotuberculosis are current sources of pathogenic strains in humans and animals. yersiniae infections occur throughout the world, but are most prevalent in regions with moderate and subtropical climates. in australia, central europe and north america, cases of human infections with yersinia enterocolitica now rank in third place. the food-processing industry may influence the epidemiological situation in different ways. effluents which contaminate the envi ... | 1991 | 1782430 |
| [proline specific peptidases and their specific inhibitors]. | several proline specific peptidases were newly isolated from mammalian organs, plants and microorganisms, and their enzymatic properties characterized. the genes of prolyl endopeptidase, aminopeptidase p and proline iminopeptidase from some microorganisms were cloned and their nucleotide sequences determined. by high expression of these genes in escherichia coli, the enzymes became possible to be used for the industrial application as well as its basic research. novel inhibitors specific for the ... | 1991 | 1783984 |
| recognition by peptide mapping of three different structural groups of outer membrane protein yop-1 of yersinia enterocolitica and yersinia pseudotuberculosis. | the structural relation of yop-1 of "european" and "american" yersinia enterocolitica serotypes o:3, o:9, o:5,27, and o:8 and o:20, respectively, and y. pseudotuberculosis serotypes i, ii, and iii was compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis and peptide mapping using staphylococcus aureus protease v8. apparent molecular weights of yop-1 ranged from 206,000 (o:3) to approx. 180,000 (o:8). according to their respective peptide maps yop-1 of the "european" and "american ... | 1991 | 1784270 |
| [the role of a plasmid with a molecular weight of 82 md in the modulation of the action of yersinia pseudotuberculosis on the proliferative activity of murine lymphoid cells]. | | 1991 | 1787842 |
| [the gene identification of bacterial species and serovariants by the polymerase chain reaction with universal oligonucleotides: the reidentification of earlier isolated strains of yersinia pseudotuberculosis]. | genetic analysis of 19 standard strains belonging to 6 yersinia species (y. pestis, y. pseudotuberculosis, y. enterocolitica, y. kirstensenii, y. frederiksenii, y. intermedia) revealed that gene typing by the method of polymerase chain reaction (pcr) with the use of universal primers permitted the identification of species in bacterial cultures by pcr patterns and the determination of y. pseudotuberculosis serovars within 4 hours. by this method 23 y. pseudotuberculosis strains (serovar 1), earl ... | 1991 | 1789029 |
| real-time monitoring of slow-wave sleep by electroencephalogram variance. | based on statistical variance as an index of electroencephalogram (eeg) parameters, we monitored slow-wave sleep in both humans and rats in real time and on-line with a widely used personal computer. this eeg variance method may be a useful tool to carry out biological rhythm research, including sleep studies. | 1991 | 1794153 |
| aspirin-like drugs, ethanol-induced rat gastric injury and mucosal eicosanoid release. | the effect of oral administration of various non-steroidal antiinflammatory drugs on ethanol-induced rat gastric injury and mucosal release of leukotriene c4, 6-keto-prostaglandin f1 alpha and 15-hydroxy-5,8,11,13-eicosatetraenoic acid was investigated. it was found that besides sodium salicylate and high doses of aspirin, other salicylate-type drugs, such as diflunisal, 4-aminosalicylic acid, 2,4-dihydroxybenzoic acid and methyl salicylate, and several non-acidic compounds, such as proquazone, ... | 1991 | 1794414 |
| viability and ultrastructure of equine embryos following culture in a static or dynamic system. | the viability and ultrastructure of equine embryos were assessed following culture in a static or perifusion system. the percentage change in diameter was greater (p less than 0.025) for embryos in the static treatment (71%) than in the perifusion treatment (33%). fluorescein diacetate (fd) scores, the percentage of fluorescing cells (fc) and fluorescent intensity (fi), also were greater (p less than 0.05 and p less than 0.01) following static culture than for embryos cultured in the perifusion ... | 1991 | 1795284 |
| memory modulation by brain benzodiazepines. | 1. recent evidence indicates that post-training memory processes are down-regulated by benzodiazepine/gaba-a systems in the amygdala, septum and hippocampus. habituation and avoidance learning are accompanied by a decrease of benzodiazepine-like immunoreactivity in the three structures, explainable by a release of benzodiazepines. immediate post-training microinjection of the benzodiazepine antagonist flumazenil into the hippocampus enhances retention of habituation. the post-training administra ... | 1991 | 1797279 |
| memory modulation by brain benzodiazepines. | 1. recent evidence indicates that post-training memory processes are down-regulated by benzodiazepine/gaba-a systems in the amygdala, septum and hippocampus. habituation and avoidance learning are accompanied by a decrease of benzodiazepine-like immunoreactivity in the three structures, explainable by a release of benzodiazepines. immediate post-training microinjection of the benzodiazepine antagonist flumazenil into the hippocampus enhances retention of habituation. the post-training administra ... | 1991 | 1797279 |
| [secondary immunodeficiency in pseudotuberculosis in man and in experiment]. | surgical material from 55 children, from 2 to 15 years of age with an abdominal form of pseudotuberculosis (appendix, lymph nodes, ileum) and the immunogenesis organs (lymphoid tissue of the small intestine, lymph nodes, appendix, spleen and thymus) of 277 rabbits infected with virulent strains of the yersinia pseudotuberculosis i serovar at the dose of 10(8) bacterial bodies were investigated. the results testify to the disturbance of all parts of the immuno-phagocytic system in pseudotuberculo ... | 1991 | 1799314 |
| a primary demyelinating disorder of young cattle. | the clinical signs and neuropathological changes are described in six limousin x calves that, at about one month after birth, showed signs of blindness, nystagmus, rotation of the eyes, opisthotonos, hyperprotraction of the forelegs and, in one case, apparent seizures. pathologically there was necrosis of the optic chiasma and focal areas of myelin sheath vacuolation or demyelination in certain areas of the brain, especially in the cerebellar peduncles. in one animal, kept alive for 7 months, th ... | 1991 | 1800911 |
| [functional state of thrombocytes in yersinia infection]. | as many as 94 patients with yersiniosis were examined for the morphofunctional status of platelets (aggregation, disaggregation, endo-exocytosis and platelet forms). the data indicate that platelet from patients with yersiniosis possess a high ability for aggregation and a low ability for disaggregation. according to the experimental and clinical data, emoxipine can restore disturbed function of human platelets. | 1991 | 1801465 |
| [the epidemiological patterns of pseudotuberculosis in khabarovsk territory]. | materials on the epidemiological characterization of pseudotuberculosis at the khabarovsk territory taken as a whole and in individual settlements, as well as on the evaluation of epizootic situation in natural foci of infection and foci created by human activities, are presented. the results of laboratory studies of vegetables, washings from environmental objects, soil specimens, material taken from rodents are demonstrated and the characterization of the virulent properties of isolated yersini ... | 1991 | 1801483 |
| [alternative localization of the determinant of pathogenicity coded by the yersinia pseudotuberculosis pvm82 plasmid]. | the chromosomal dna regions in yersinia pseudotuberculosis strains occur that are homologous to 25 md dna segment of the plasmid pvm82 encoding the bacterial capability of immunosuppression. the character of the chromosomal dna regions dispersion reacting with the 25 md segment probes is different in epidemiologically hazardous and nonvirulent strains of yersinia pseudotuberculosis. the specific dna regions occur as well as identical ones. the suppression of antibody formation to a number of mai ... | 1991 | 1808508 |
| [the clinico-allergic indices of yersiniosis patients]. | eighty patients with yersiniosis (40 with intestinal yersiniosis, 40 with pseudotuberculosis) were examined for clinical and allergic characteristics (collection of allergological histories, identification of the most common allergic symptoms of the disease and determination of the index of mast cell degranulation). according to the data obtained, the patients with yersiniosis are admitted to the hospital with an aggravated premorbid condition in the form of body allergization, in whose origin b ... | 1991 | 1810058 |
| [the characteristics of pseudotuberculosis with joint involvement in the presence of chronic opisthorchiasis]. | a study was made of the clinical picture, certain laboratory and immunological findings in pseudotuberculosis patients with involved joints in the presence of opisthorchiasis invasion. the patients suffering from pseudotuberculosis and chronic opisthorchiasis frequently demonstrated icteric sclera, erythema nodosum, skin itch, tenderness in the right hypochondrium, hepatomegaly, liquid stool, leukopenia, eosinophilia, hyperbilirubinemia, activation of aminotransferases. later they showed the dia ... | 1991 | 1810061 |
| [bacterial flora of the digestive tract of specimens of notothenia neglecta caught in caleta potter (south shetland archipelago, antarctica)]. | a qualitative and quantitative study of the predominant heterotrophic bacterial flora in the stomach and intestine of the antarctic fish notothenia neglecta was carried out: 10 newly caught specimens (potter cove, king george island, south shetland islands) were analyzed. the cultures were made under aerobic and anaerobic conditions. the stomach flora showed variable results between samples which are probably related to the flora ingested with food. the gut flora was composed almost exclusively ... | 1991 | 1815278 |
| [specific proteolysis by fibrinolysin-coagulase from yersinia pestis of yersinia pseudotuberculosis outer membrane proteins coded by the ca(2+)-dependence plasmid]. | the pesticinogenicity 9.5 kb plasmid from yersinia pestis strain ev76 has been marked by the kanamycin phosphotransferase gene inserted into psti site and designated pp3. the obtained plasmid pp3 determines the synthesis of 45 kd pesticin, alpha and beta-forms of fibrinolysin coagulase (37 and 35 kd) and the 29, 19 and 13 kd proteins in escherichia coli mini cells. when transferred into yersinia pseudotuberculosis strain 6933 the plasmid causes the proteolysis of outer membrane proteins. the 150 ... | 1991 | 1827173 |
| [biochemical and functional characteristics of bladder muscarinic receptors and effects of experimental diabetes in rats]. | bladder dysfunction is a common complication of diabetes mellitus and is attributed in part to peripheral neuropathy. voiding function is mainly controlled by muscarinic receptor function. therefore, i investigated first the biochemical and functional characteristics of urinary bladder muscarinic receptors and then the effects of experimental diabetes on them. experimental diabetes was induced in 2 month-old male rats by intravenous injection of 65 mg/kg of streptozotocin (stz). effects of diabe ... | 1991 | 1828510 |
| [biochemical and functional characteristics of bladder muscarinic receptors and effects of experimental diabetes in rats]. | bladder dysfunction is a common complication of diabetes mellitus and is attributed in part to peripheral neuropathy. voiding function is mainly controlled by muscarinic receptor function. therefore, i investigated first the biochemical and functional characteristics of urinary bladder muscarinic receptors and then the effects of experimental diabetes on them. experimental diabetes was induced in 2 month-old male rats by intravenous injection of 65 mg/kg of streptozotocin (stz). effects of diabe ... | 1991 | 1828510 |
| [axillary lymphadenitis due to yersinia pseudotuberculosis]. | | 1991 | 1830152 |
| yersinia pseudotuberculosis isolation from cockatoo. | | 1991 | 1830763 |
| [pseudotuberculosis in the krasnodar territory. the clinico-epidemiological characteristics of its morbidity and the biological properties of its strains]. | group cases of pseudotuberculosis, first registered at health-restoring institutions of the health resort of gelendzhik (the krasnodar territory), are described. the etiology of these cases of the disease was established on the basis of clinico-epidemiological data, specific seroconversion in 66% of the examined patients and the isolation of yersinia pseudotuberculosis strains from the feces of four patients. the combination of signs observed in the isolated strains, viz. the presence of plasmid ... | 1991 | 1831948 |
| the yersinia pseudotuberculosis invasin protein and human fibronectin bind to mutually exclusive sites on the alpha 5 beta 1 integrin receptor. | the yersinia pseudotuberculosis invasin protein promotes bacterial penetration into mammalian cells by binding to several beta 1 chain integrins. we show here that proteins containing the cell-binding domain of invasin bind to the fibronectin receptor alpha 5 beta 1 isolated from human placenta and immobilized on a filter membrane. two forms of the receptor, each having a molecular weight of about 290,000, were immunodepleted by monoclonal antibodies specific for the beta 1 subunit or the alpha ... | 1991 | 1837020 |
| genetic analysis of homology between the virulence plasmids of salmonella dublin and yersinia pseudotuberculosis. | two segments within the virulence region of salmonella dublin plasmid psdl2 that were homologous to regions on yersinia pseudotuberculosis plasmid pib1 were located with regard to the four known genes (vsda, vsdb, vsdc, and vsdd) of psdl2. one segment mapped upstream of vsda within an insertion element related to is630 of shigella sonnei; the second was confined to a 45-bp sequence containing an inverted repeat between vsdc and vsdd. on pib1, both areas were located in an intergenic region upstr ... | 1991 | 1840573 |
| humoral immune response in dogs experimentally infected with trypanosoma cruzi. | | 1991 | 1842441 |
| yersinia pseudotuberculosis infection as a cause of arthritis in children. | | 1991 | 1845401 |
| cyclic amp-elevating agents block chemoattractant activation of diradylglycerol generation by inhibiting phospholipase d activation. | agents which elevate cellular camp (prostaglandin e2, theophylline, and forskolin) or mimic camp action (dibutyryl camp) are known to inhibit human neutrophil activation (superoxide generation and secretion) by receptor-linked agonists such as formyl-methionyl-leucyl-phenylalanine (fmlp). herein, we show that these agents also markedly inhibit fmlp-stimulated diradylglycerol generation (assayed by mass methods). the magnitude of inhibition correlated with the ability of a given agent or combinat ... | 1991 | 1847376 |
| relevance of n-nitrosamines to oesophageal cancer in china. | oesophageal cancer occurs at a very high frequency in certain areas of china, especially in linxian county, henan province. previous studies suggested that n-nitroso compounds play a causative role. in order to study further the exposure of linxian inhabitants, the intake of n-nitrosamines in the diet was determined. the total daily intake of volatile nitrosamines and of n-nitroso-n-methylbenzylamine (nmbza) was higher in linxian than in two other counties. nmbza can induce cancer in animal and ... | 1991 | 1855832 |
| in vivo strain in cranial sutures: the zygomatic arch. | although cranial sutures presumably play a role in absorbing and/or transmitting loads applied to the skull, loading patterns on facial sutures are poorly understood. the zygomatic arch provides a comparatively isolated mechanical part of the skull containing a single suture, the zygomatico-squamosal. in pigs the zygomatico-squamosal suture has a short vertical segment located within the postorbital process and a longer horizontal segment which extends posteriorly. in anesthetized pigs single-el ... | 1991 | 1856873 |
| in vivo strain in cranial sutures: the zygomatic arch. | although cranial sutures presumably play a role in absorbing and/or transmitting loads applied to the skull, loading patterns on facial sutures are poorly understood. the zygomatic arch provides a comparatively isolated mechanical part of the skull containing a single suture, the zygomatico-squamosal. in pigs the zygomatico-squamosal suture has a short vertical segment located within the postorbital process and a longer horizontal segment which extends posteriorly. in anesthetized pigs single-el ... | 1991 | 1856873 |
| the surface-located yopn protein is involved in calcium signal transduction in yersinia pseudotuberculosis. | the low-calcium response (lcr) is strongly conserved among the pathogenic yersinia species and is observed when the pathogen is grown at 37 degrees c in ca(2+)-depleted medium. this response is characterized by a general metabolic downshift and by a specific induction of virulence-plasmid-encoded yop genes. regulation of yop expression is exerted at transcriptional level by a temperature-regulated activator and by ca(2+)-regulated negative elements. the yopn gene was shown to encode a protein (f ... | 1991 | 1857212 |
| [the effect of yersinia pestis ev76 6 md plasmid on the composition of outer membrane proteins of yersinia pseudotuberculosis ypiii]. | on the basis of yersinia pseudotuberculosis strain ypiii the isogenic variants containing the different combinations of 47 md plasmids from yersinia pestis or yersinia pseudotuberculosis cells with the 6 md pyp plasmid from yersinia pestis ev (intact or having impaired the pla gene determining the synthesis of plasmocoagulase). the degradation of the secreted proteins encoded by the 47 md plasmids of yersinia pestis and yersinia pseudotuberculosis in the cells harbouring the 6md pyp plasmid has ... | 1991 | 1857369 |
| [detection of virulence markers in clinical strains of yersinia]. | the dispersion of plasmid pyv associated virulence markers in 474 yersinia strains isolated from people has been studied. the ability to autoagglutination, calcium dependence of growth and the specific antigens were identified in 157 strains of traditionally pathogenic yersinia enterocolitica serovars 03, 09, yersinia pseudotuberculosis serovar i. they were not found in 223 strains of other 12 serovars of yersinia enterocolitica, in 40 strains of yersinia frederiksenii, yersinia kristensenii, ye ... | 1991 | 1857370 |
| [the effect of a plague pathogen plasmid on lethality and immunogenicity]. | on the model of yersinia transconjugants (yersinia pestis, yersinia pseudotuberculosis, yersinia enterocolitica) carrying the conjugative cointegrates containing the 47 and 65 md plasmids from yersinia pestis the data were obtained on the different affects of the latter plasmids on the lethality and immunogenicity conferred by the host bacterial cells. the plasmid effects were drastically different during bacterial infection in mice or guinea pigs. the possibility of appearance of the recombinan ... | 1991 | 1857372 |
| [the effect of the bacterial genome on the expression and behavior of the plasmid determining the ca2+-dependence of the plague pathogen]. | the conjugative cointegrate containing the 47 md plasmid of yersinia pestis has been transferred into the strains of the different yersinia (yersinia pestis, yersinia pseudotuberculosis, yersinia enterocolitica) and escherichia coli ca. there appeared in the populations of recombinant yersinia under the conditions of ca2+ deficit at 37 degrees c the cells coming into the stasis stage or dying. it was shown on the model of yersinia enterocolitica that bacterial lethality might be prevented by exc ... | 1991 | 1857373 |
| [the heterogeneity of collection strains of yersinia pseudotuberculosis and their molecular biological properties]. | the capacity of y. pseudotuberculosis strains for disassociation with the appearance of s- and p-forms has been studied. strains 852 and 9547 show high stability in s-forms, their conversion into r-forms occurring at 40-42 degrees c. strain 6953 shows pronounced polymorphism and instability of its associations at different growth temperatures. strain 9532 exists in s- and r-forms which retain their stability during numerous subculturings at different growth temperatures and prolonged storage. th ... | 1991 | 1858469 |
| [the nonspecific changes in the immune system to the administration of the membrane-ribosomal fraction of the causative agent of pseudotuberculosis]. | nonspecific changes in different elements of the immune system of animals under the action of y. pseudotuberculosis membrane-ribosomal fraction (mrf) with high protective potency have been studied for the purpose of the analysis of the preparation for immunological safety. mrf has been shown to produce no changes in humoral immune response to antigens of different nature or to enhance this response, to produce no essential effect on the intensity of the reaction of delayed hypersensitivity to sh ... | 1991 | 1858471 |
| [structure of the o-specific polysaccharide chain of the yersinia pseudotuberculosis lipopolysaccharide (serovar ii c)]. | an o-specific polysaccharide has been isolated on mild acid hydrolysis of lipopolysaccharide from yersinia pseudotuberculosis serovar iic and shown to consist of abequose, d-mannose and 2-acetamido-2-deoxy-d-galactose residues in the ratio 0.8:3:1. from the results of acid hydrolysis, 13c nmr, methylation and periodate oxidation studies the structure of the repeating unit of the o-specific polysaccharide is deduced as follows: (formula; see text) | 1991 | 1863285 |
| acute mesenteric lymphadenitis due to yersinia pseudotuberculosis lacking a virulence plasmid. | a serotype 4a strain of yersinia pseudotuberculosis lacking the virulence plasmid pyv (pyv- strain) was isolated from the mesenteric lymph nodes but not from the stool or the appendix of a 10-year-old girl with a diagnosis of acute mesenteric lymphadenitis. microscopically, reticulocytic abscess and lymphadenitis were persent in the enlarged mesenteric lymph nodes. antibody against the isolate was detected in the serum. the isolate was negative for the presence of plasmid pyv and plasmid pyv-med ... | 1991 | 1864948 |
| allergic fungal sinusitis: problems in diagnosis and treatment. | although first described in 1983, allergic aspergillus sinusitis (aas) has yet to gain wide recognition among otolaryngologists and pathologists. we have treated three patients with a history of asthma, nasal polyposis, and recurrent pansinusitis who fit the description of allergic aspergillus sinusitis. histopathologically, mucinous material with abundant eosinophils and charcot-leyden crystals ("allergic mucin") is interspersed with fungal hyphae. immunologic characteristics include serum tota ... | 1991 | 1865728 |
| different patterns of behavior produced by haloperidol, pentobarbital, and dantrolene in tests of unconditioned locomotion and operant responding. | three motor-impairing drugs with different putative mechanisms of action (haloperidol 0.00, 0.075, 0.15, 0.30 mg/kg ip; pentobarbital 0.00, 4.5, 9, 12 mg/kg ip; and dantrolene 0.00, 5, 7.5, 10 mg/kg ip) produced strikingly similar patterns of dose-dependent attenuation in unconditioned locomotor behavior. however, the same drugs and doses produced highly divergent patterns of disruption when tested using different groups of rats in a food-rewarded operant task, which included both response initi ... | 1991 | 1876659 |