| nosocomial klebsiella infections in an intensive care nursery. | nosocomial infections in an intensive care nursery (icn) due to kanamycin-resistant klebsiella pneumoniae during a four-month period with an infection rate of 12% and mortality of 47% are reported. emergence of this organism was thought to be due to the widespread use of kanamycin. transmission probably occurred via hands of personnel contaminated during care of colonized or infected infants. control measures advocated are better handwashing technic and discriminate use of antibiotics. this repo ... | 1977 | 320669 |
| observations on a spontaneous case of fatal ovine mastitis due to klebsiella pneumoniae. | | 1977 | 320795 |
| interaction of blood with enterobacteriaceae: hemolysis, hemagglutination, fibrinolysis. | one thousand eighty enterobacteriaceae, isolated during a period of three months, were tested for the presence of hemolysis, hemagglutination and fibrinolysis on human erythrocytes and plasma. the fibrinolysis on human erythrocytes and plasma. the escherichia coli have the greatest diversity of action on the blood. of 481 strains, 32.9% were hemolytic, 21.9% fibrinolytic, and 10.5% hemagglutinating. of 190 strains of klebsiella, 95.2% were fibrinolytic. of 53 strains of enterobacter, 90.6% were ... | 1977 | 320861 |
| mechanism of action of adenosylcobalamin: glycerol and other substrate analogues as substrates and inactivators for propanediol dehydratase--kinetics, stereospecificity, and mechanism. | a number of vicinal diols were found to react with propanediol dehydratase, typically resulting in the conversion of enzyme-bound adenosylcobalamin to cob(ii)alamin and formation of aldehyde or ketone derives from substrate. moreover, all are capable of effecting the irreversible inactivation of the enzyme. the kinetics and mechanism of product formation and inactivation were investigated. glycerol, found to be a very good substrate for diol dehydratase as well as a potent inactivator, atypicall ... | 1977 | 321014 |
| immune complex glomerulonephritis associated with klebsiella pneumoniae infection. | the kidneys of three patients who died of pneumonia due to klebsiella pneumoniae were studied at autopsy by light and immunofluoerescent microscopy. one had no clinical evidence of renal disease; two had only microscopic hematuria and mild proteinuria. light microscopy revealed focal proliferative glomerulonephritis in all three cases. also in all three, immunofluorescent microscopy revealed a granular deposition of capsular polysaccharide antigens of klebsiella pneumoniae in association with im ... | 1977 | 321171 |
| studies on limulus amoebocyte lysate. isolation of pro-clotting enzyme. | a pro-clotting enzyme capable of causing the gelation of clottable proteins in limulus polyphemus (horseshoe crab) has been purified to apparent homogeneity as judged by sodium dodecyl sulfate-gel electrophoresis. the activation of the pro-clotting enzyme depended on the presence of both ca+ and endotoxin. it contained gamma-carboxyglutamic acids and gave a single nh2-terminal lysine. the enzyme was inhibited by diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, and soy bean trypsin inh ... | 1977 | 321451 |
| drug resistance in relation to use of silver sulphadiazine cream in a burns unit. | topical chemoprophylaxis of extensive burns with silver sulphadiazine cream led to a large increase in the proportion of sulphadiazine-resistant gram-negative bacilli in a burns unit. when all sulphonamide treatment in the ward was stopped; the incidence of sulphonamide-resistant strains fell back to levels similar to those recorded when silver sulphadiazine treatment was introduced. this was associated with a large reduction in the incidence of resistance of certain gram-negative bacilli (espec ... | 1977 | 321478 |
| [doxycycline and minocycline hc1; a comparative study]. | | 1977 | 321980 |
| neonatal septicemia due to klebsiella pneumoniae type 60. epidemic of unusually low virulence. | | 1977 | 321994 |
| resistance to gentamicin: a growing concern. | gentaminic was introduced in 1969 as a broad-spectrum aminoglycoside effective in vitro against a majority of aerobic gram-negative bacilli. in recent years gentamicin-resistant clinical isolates have become more prevalent. in our laboratory in 1975, 32% of pseudomonas sp and 44% of indole-negative proteus sp isolates were resistant to gentamicin. resistance to tobramycin is also increasing; 24% of escherichia coli and 28% of indole-negative proteus sp isolates were found to be tobramycin-resist ... | 1977 | 322294 |
| septicemia from heparin-lock needles. | | 1977 | 322567 |
| [immunostimulating activities in vivo of extract from klebsiella pneumoniae]. | the adjuvant and immunostimulating activities of a glycoprotein preparation (gp) extracted from klebsiella pneumoniae have been studied. when injeted with an ovalbumin in incomplet freund adjuvant emulsion, the gp induces delayed hypersensitivity to the antigen and increases the level of antibody developed. when it is injected before the antigen to c57bl/6 mice, the gp provokes an increase of plaque forming cells, rosette forming cells, and antibody responses. with the doses that are used, no me ... | 1977 | 322582 |
| [new cephalosporin antibiotic: cephapirin]. | | 1977 | 322931 |
| preparation of 125i-labeled oligosaccharide derivatives with the aid of 3-(4-hydroxyphenyl)propionic acid n-hydroxysuccinimide ester. | | 1977 | 323044 |
| expression of klebsiella pneumoniae nitrogen fixation genes in nitrate reductase mutants of escherichia coli. | nitrate reductase (nar) a, b and e mutants of escherichia coli with plasmids carrying klebsiella pneumoniae nitrogen fixation (nif) genes reduced acetylene independently of added molybdate, but nar d mutants showed pleiotropic dependence on the concentration of added molybdate for expression of both nar and nif. no complementation of nar mutations by nif occurred; nitrite but not nitrate repressed nif in nar hosts. derepression of nif occurred in molybdenum-deficient nar d (nif) strains since ni ... | 1977 | 323414 |
| role of radiosensitive and radioresistant cells in nonspecific resistance to infection of lps-treated mice. | | 1977 | 323480 |
| crepitant cellulitis and myonecrosis caused by klebsiella. | a fatal case of crepitant cellulitis with myonecrosis, the first caused by klebsiella pneumoniae, is reported. the infection started in the left thigh but progressed rapidly despite appropriate antibiotics and surgery, which included incision and drainage and later a left-hip disarticulation. this case emphasizes that nonclostridial crepitant cellulitis is potentially severe and that the presence of myonecrosis is an indication for early radical surgery. | 1977 | 323519 |
| [treatment for sepsis in hematological diseases with combined large-dosage antibiotics of two different groups, with special reference to the relation between clinical effects and drug-sensitivity of causative organisms (author's transl)]. | | 1977 | 323539 |
| gram-negative bacillary pneumonia in the compromised host. | the clinical and radiological characteristics of 217 consecutive episodes of gram-negative bacillary pneumonia occurring in 189 adult cancer patients between november 1968 and december 1974 were analyzed. the majority of patients had acute leukemia (54%). fever larger than or equal to 101 degrees f was the single most common symptom and sign of the presence of infection (90%). next in frequency were crepitant rales (65%), cough (41%), dyspnea (19%) and chest pain (18%). radiographic evidence of ... | 1977 | 323640 |
| isolation of klebsiella ozaenae and klebsiella rhinoscleromatis in a general hospital. | klebsiella ozaenae and klebsiella rhinoscleromatis are uncommon organisms usually associated with granulomatous or necrotizing disease of the upper airways. seven patients are described, non of whom had characteristic clinical manifestations of infection with these species. the organisms were recovered from mixed wound infections or sputum of five patients, and from the blood of three patients. antibiotic sensitivity patterns were unusual and included susceptibility to both ampicillin and carben ... | 1977 | 324264 |
| a serologic response in human infection with enterobacteriaceae. | sera from patients infected with escherichia coli, proteus, klebsiella, and serratia were studied for precipitins to ultrasonic extracts of these organisms in gel-diffusion plates. sera from 66 per cent of these patients contained precipitins when initially tested. twenty-four per cent of sera tested in the first week after onset of infection contained precipitins, but this rose to 78 per cent by the third week. cross-reactions of sera with pseudomonas antigens were unusual, but were common with ... | 1977 | 324278 |
| cefazaflur, a new parenteral cephalosporin: in vitro studies. | cefazaflur was tested in vitro against 262 strains of bacteria. inhibitory and bactericidal concentrations were determined with two inoculum sizes of bacterial cells in mueller-hinton broth and nutrient broth. agar dilution studies also were performed. when tested in agar, 5.0 mug or less of cefazaflur per ml inhibited almost all strains of staphylococcus aureus, escherichia coli, klebsiella, and proteus mirabilis. the drug was less active against enterobacter and indole-positive proteus, and 7. ... | 1977 | 324399 |
| sequential hospitalwide outbreaks of resistant serratia and klebsiella infections. | late in 1973 at the nashville veterans administration hospital, an intrusion of serratia marcescens infections that were resistant to gentamicin sulfate and other antimicrobial agents occurred. this abated somewhat, only to be superseded by another wave of multiply-resistant infections due to klebsiella pneumoniae beginning in the spring of 1974. approximately 400 patients had substantial infections with these organisms during the 2 1/4 year period, imposing considerable morbidity and mortality. ... | 1977 | 324421 |
| nosocomial epidemics seriatim. multidrug-resistant bacteria and r factors. | | 1977 | 324422 |
| outbreaks of serratia and klebsiella infections. | | 1977 | 324424 |
| relation of colonial morphologies in soft agar to morphological and biological properties of the k-9 strain of klebsiella pneumoniae and its variants. | during repeated subcultures of strain k-9 of klebsiella pneumoniae, three variants, a, b, and c, were obtained showing different colonial morphologies in soft agar. the parent strain k-9 produced colonies that were large, smooth, and globular; variant a, wedged balloon forms somewhat elongate; variant b, rain-drop-like with some streaming; and variant c, small compact rough globules. these colonial forms in soft agar medium correlated well with the size of capsule determined by cell volume index ... | 1977 | 324587 |
| pollution indicator bacteria associated with municipal raw and drinking water supplies. | a total of 3819 bacterial cultures isolated from municipal water samples were identified using a combination of enterotubules and confirmatory media. frequency distributions for the different genera or groups of bacteria were similar for raw water and drinking water isolations, except for escherichia organisms which doubled their frequency in raw water. differences between the membrane filter (mf) and presence-absence (p-a) test with regard to types of organisms isolated were limited to klebsiel ... | 1977 | 324588 |
| cephalexin: clinical and laboratory evaluation in infants and children. | cephalexin appears to be a highly effective antibiotic. it possesses the qualities of rapid oral absorption, production of high drug levels in the blood and urine, and near absence of side effects. it is effective against infections due to gram-positive cocci infections, with the exception of enterococcus, and for most infections caused by e. coli and klebsiella. it is useful in the treatment of a wide variety of infections in infants and children, and particularly valuable with susceptible infe ... | 1977 | 324691 |
| in vitro susceptibility of cephalothin-resistant enterobacteriaceae to cefoxitin and bl-s786. | the in vitro antibacterial activity of two agents relatively resistant to beta-lactamases, bl-s786 and cefoxitin, was tested against 123 recent different clinical isolates of cephalothin-resistant enterobacteriaceae. bl-s786 showed considerable activity against escherichia coli and lesser activity against klebsiella pneumoniae with, respectively, 68% and 41% inhibited at 32 microng/ml. cefoxitin showed more activity in vitro against e. coli, k. pneumoniae, serratia marcescens and providencia stu ... | 1977 | 324961 |
| susceptibility of penicillins and cephalosporins to beta-lactamases assessed by a new test. | | 1977 | 324965 |
| a microbiological investigation of acute summer gastroenteritis in black south african infants. | a microbiological investigation of black infants suffering from severe acute summer gastroenteritis revealed enteropathogenic agents in 30 out of 37 patients (81%). enterotoxigenic bacteria were isolated from 15 patients (41%). a total of 16 enterotoxigenic strains were isolated, comprising 9 enterotoxigenic escherichia coli strains secreting labile and stable toxin on their own and in combination, and labile-toxin secreting strains of klebsiella pneumoniae (4), enterobacter cloacae (2) and prot ... | 1977 | 325127 |
| [present situation of gram-negative bacillus infections in pediatrics]. | | 1977 | 325249 |
| enhancement of nonspecific immunity to klebsiella pneumoniae infection by a synthetic immunoadjuvant (n-acetylmuramyl-l-alanyl-d-isoglutamine) and several analogs. | n-acetylmuramyl-l-alanyl-d-isoglutamine and four other synthetic adjuvants that are structural analogs of part of the mycobacterial peptidoglycan monomer are shown to enhance the nonspecific immunity of mice infected by klebsiella pneumoniae. these compounds are active by various routes, including oral administration; they are also effective when administered after challenge. of the seventeen other analogs tested, none is able to increase significantly resistance to infection, although seven of ... | 1977 | 325566 |
| [growth of non-sporing anaerobes in an oxygen-free blood culture system (author's transl)]. | the efficacy of the commercially available vacutainer blood culture system to support the growth of non-sporing anaerobes was compared with two laboratory-prepared blood culture media (supplemented thioglycollate medium and brain heart infusion). the media were inoculated with 10, 100 and 1000 organisms of the species tested, and the number of colony-forming units was determined at intervals of 8-10 hrs. analogous experiments were performed with batches of the media to which 10% vol/vol of human ... | 1977 | 325956 |
| [checking the reliability of the pathotec biochemical test system for bacterial identification]. | tests of the pathotec system intended for express bacteriological diagnosis were checked in comparative experiments with the common biochemical methods. cultures of the following microbes were used: schigella, salmonella, escherichia, citrobacter, klebsiella, enterobacter, proteus, providencia, pseudomonas, bordetella, staphylococcus, streptococcus. in a number of tests, such as determination of cytochromoxidase, nitrate reduciase, phenylalaninedeaminase, indol, acetoin (for the differentiation ... | 1977 | 325964 |
| klebsiella pneumoniae nosocomial epidemic in an intensive care nursery due to contaminated intravenous fluid. | | 1977 | 326033 |
| the use of bile - esculin agar for the taxonomic classification of the family enterobacteriaceae. | bile-esculin medium has been used for many years for the presumptive identification of group d streptococcus. the test is based on the ability of a bacterium to grow in the presence of 40% bile and produce esculinase. 2935 strains of enterobacteriaceae were inoculated onto bile-esculin agar slants and incubated at 35 c. esculin hydrolysis was determined after 24 and 48 hours. at 24 hours of incubation esculin hydrolysis was limited to the genera klebsiella, enterobacter, serratia, and the specie ... | 1977 | 326178 |
| klebsielleae in drinking water emanating from redwood tanks. | a survey was made of the bacteriological quality and chlorine content of 33 public and private water systems that utilize redwood storage tanks. coliforms of the genera klebsiella and enterobacter were isolated from 9 of 10 private drinking water systems and from 11 of 23 water systems in state and federal parks. total coliform counts in the private systems exceeded federal membrane filter guidelines by as much as 10-to 40-fold. coliform counts were highest in the newer reservoirs. factors contr ... | 1977 | 326189 |
| quantitative structure-activity relationships. v. a simple simple algorithm for fujita-ban and free-wilson analyses. | for quantitative structure-activity analyses a simple algorithm for the calculation of de novo group contributions by fujita-ban analysis is given. this algorithm corresponds in all details to a computer program for linear multiple regression analysis. however, the transformation of the original matrix to the normal equations matrix is easily achieved without a computer. the normal equations matrix can be solved with a modern desk calculator being equipped with a matrix rom. two examples are giv ... | 1977 | 326263 |
| enteric flora of normal laboratory guinea-pigs. | the bowel flora of guinea-pigs was studied using non-selective cultural conditions and conditions highly selective for escherichia coli organisma. no e. coli were cultured from adult or juvenile bowel content. parallel studies showed that culture media supported the growth of small numbers of reference e. coli "seeded" into them along with bowel content. it is concluded that normal adult laboratory guinea-pigs may be bacteriologically equivalent to the ncs strain of "e. coli-free" mice. | 1977 | 326289 |
| the capsular network of klebsiella pneumoniae. | attempts at improving chemical fixation for electron-microscopic observation of the capsule of klebsiella pneumoniae were made. the capsule was preserved by using alcian blue - lanthanum and tris-(1-aziridinyl) phosphine oxide (tapo) - aldehyde - osmium procedures. despite the different retention of the overall capsular material and minor variations in morphological details, in both cases the interpretation of ultrastructural patterns suggested that the capsule be composed of a meshed network of ... | 1977 | 326360 |
| superoxide dismutase in anaerobic bacteria of clinical significance. | twenty-two anaerobic bacteria isolated from infected sites and normal fecal flora were assayed for superoxide dismutase (sod). the organisms were also classified according to their oxygen tolerance into aerotolerant, intermediate, and extremely oxygen-sensitive groups. there was a correlation between the enzyme level and the oxygen tolerance, in that the aerotolerant and intermediate organisms had sod, whereas the extremely oxygen-sensitive isolates had low or undetectable enzyme. among the oxyg ... | 1977 | 326669 |
| lactoferrin as a factor of resistance to infection of the bovine mammary gland. | | 1977 | 326739 |
| further evaluation of the test for detection of antibody-coated bacteria in urine sediment. | to assess the reproducibility of the test for detection of antibody-coated bacteria in urine sediment as it might be used in a diagnostic laboratory for classification of urinary tract infections, multiple urine specimens from 83 patients were tested. the results were reproducible and consistent, or if inconsistent potentially explainable, in all but four patients. the explanation for inconsistencies include the immune response to the infecting bacteria, nonspecificity of the antibody coating th ... | 1977 | 326807 |
| occurrence of disseminated intravascular coagulation (dic) in obstructive jaundice and its relation to biliary tract infection. | coagulation studies were done on 78 consecutive cases of obstructive jaundice with or without biliary tract infection. among 26 cases with biliary tract infection 20 cases showed no bleeding tendency but remarkable hypercoagulability with decreased fibrinolytic activity. other six cases developed diffuse bleeding tendency in addition to the signs of hypotension and multiorgan dysfunction such as oliguria, respiratory distress and mental confusion. most showed marked coagulation defects character ... | 1977 | 327128 |
| synthetic disaccharide-protein antigens for production of specific 04 and 09 antisera for immunofluorescence diagnosis of salmonella. | the synthetic disaccharides abequose 1 leads to a 3 mannose and tyvelose 1 leads to a 3 mannose, representative of salmonella o-antigen 4 and 9 respectively, were covalently linked to bovine serum albumin (bsa) . antisera from rabbits immunized with these immunogens were used in indirect immunofluorescence assay for the identification of group b (o-antigen 4) and d (o-antigen 9) salmonella. a total of 1030 enteric bacterial strains were tested, including 207 group b and 55 group d salmonella. th ... | 1977 | 327249 |
| [influence of prevention using antibiotics on the infection pathogens of intensive care patients and the flora of intensive care units]. | | 1977 | 327296 |
| the mutagenic action of nitroimidazoles. ii. tinidazole, ipronidazole, panidazole and ornidazole. | the 5-nitroimidazoles tinidazole (fasigyn), ipronidazole (ro-7-1554), panidazole and ornidazole (tiberal, ro-7-0207) in concentrations of 0.02--1 mm per liter increased the mutation frequency of klebsiella pneumoniae. escherichia coli k12 and citrobacter freundii to streptomycin resistance, including streptomycin dependence, in luria and delbrück's fluctuation test. at low concentration (0.1 mm), the increase of the mutation frequency caused by each compound was nearly the same, i.e. 3--4 times ... | 1977 | 327306 |
| segregation of genes for nitrogen fixation into minicells of escherichia coli. | | 1977 | 327484 |
| [studies on the mastitis-metritis-agalactia syndrome (milk fever) in sows. ii. bacteriological findings in spontaneous cases]. | | 1977 | 327538 |
| clinical experience with amikacin, a new aminoglycoside antibiotic. | amikacin, a new semisynthetic aminoglycoside antibiotic, was administered parenterally to 20 patients suffering from severe gram-negative bacterial infections. the antibiotic was found to be highly effective in controlling infection. it was well tolerated and no signs of nephro- or otoxicity were observed. the few side-effects which were seen appeared to be dose-related. the recommended 12-hourly dosage regimen has also proved useful in medium- to long-term management of refractory pulmonary inf ... | 1977 | 327583 |
| coliform and pseudomonad mastitis: epidemiology and control. | | 1977 | 327673 |
| gentamicins. | gentamicin is a new broad-spectrum antibiotic, basic and water-soluble, produced and developed by schering corporation-bloomfield, new jersey (1967 and 1968). it is produced by micromonospora purpurea, a member of a genus of microorganisms from which no other antibiotics have been derived. paper chromatographic techniques showed the components of gentamicin complex designated as c', c'a, and c2. gentamicins are bactericidal antibiotics, active in vivo in low concentrations against a wide spectru ... | 1977 | 327729 |
| [treatment with chloranphenicol of neonatal sepsis-meningitis by gram-negative bacteria (author's transl)]. | eight newborn infants having previously received broad spectrum antibiotics were treated with intravenous chloranphenicol succinate for sepsis-meningitis during a klebsiella neumoniae outbreak. five of them survived without sequelae. over the third week of treatment two infants developed a transient bone marrow suppresion accompanied by a "pseudo-septic" clinical picture; this syndrome, that we have not found previously reported, was benign, disappearing when the drug was discontinued. | 1977 | 327883 |
| beta-lactamase-directed barrier for penicillins of escherichia coli carrying r plasmids. | strains of escherichia coli and salmonella typhimurium carrying r plasmids, which were obtained from ampicillin-resistant clinical isolates of e. coli and klebsiella spp. and specified either the type iiia (tem-type) or type va (oxacillin-hydrolyzing) beta-lactamase, are resistant not only to ampicillin but also to carbenicillin and sulbenicillin. the latter two derivatives, however, are poorly hydrolyzed in vitro by the beta-lactamases. although values of k(m) of the enzymes are lower for sulbe ... | 1977 | 327924 |
| mercury resistance and r plasmids in escherichia coli isolated from clinical lesions in japan. | the mercury and antibiotic resistance of 338 strains of escherichia coli isolated from hospital patients was determined. resistance to mercury was found in 58.6% of the isolates. the frequencies of resistance to streptomycin (sm), tetracycline (tc), chloramphenicol (cm), kanamycin (kan), cephaloridine (cer), and gentamicin (gm) were 66.3, 60.3, 56.5, 42.9, 32.1, and 1.5%, respectively. among the above, 198 mercury- and antibiotic-resistant isolates were selected and tested for their ability to t ... | 1977 | 327927 |
| nitrogenase of klebsiella pneumoniae. water proton nmr relaxation studies on the binding of divalent metal ions and nucleotides to the iron protein. | interactions between the iron protein, kp2, of nitrogenase manganese ions, magnesium ions, and the nucleotides atp or adp, have been studied in aqueous solution by monitoring the water proton nmr relaxation rate enhancement caused by mn2+. binding of mn2+ to a molecule of kp2 occurs at four sites, indistinguishable within experimental error, having a kd = 350 +/- 50 micron. the mn2+ - kp2 complex has a low characteristic enhancement (epsilonb = 6 +/- 0.5). all four sites can alternatively bind m ... | 1977 | 328053 |
| microbial adjuvant and autoimmunity. ii. production of lesions in mice immunized with syngeneic tissue extracts together with the capsular polysaccharide of klebsiella pneumoniae. | | 1977 | 329062 |
| host factor for coliphage q beta rna replication: presence in procaryotes and association with the 30s ribosomal subunit in escherichia coli. | the host factor required for in vitro coliphage q beta rna replication, a heat-stable rna binding protein present in uninfected escherichia coli, has been detected by both immunological and functional tests in acinetobacter calcoaceticus, klebsiella pneumoniae, pseudomonas aeruginosa and pseudomonas putida. it was not detectable by these criteria in bacillus stearothermophilus, bacillus subtilis, caulobacter crescentus, micrococcus lysodeikticus, rhodopseudomonas capsulata or saccharomyces cerev ... | 1977 | 329101 |
| protection against experimental necrotizing enterocolitis by maternal milk. i. role of milk leukocytes. | a rat model of necrotizing enterocolitis of the neonate in which maternal milk had been protective was studied to determine what components of the milk afforded protection and by what mechanism. frozen and thrawed rat milk was not protective, but formula supplemented with rat milk cells was. it was concluded that the cells provided protection. the cells, which are principally mononuclear phagocytes, can phagocytize and kill the klebsiella pneumoniae strain used in the animal model. animals with ... | 1977 | 329204 |
| [case of cerebrospinal meningitis caused by friedlaender's bacillus in a 4-month-old infant]. | | 1977 | 329208 |
| anaerobic and aerobic bacteriology in head and neck cancer surgery. | a prospective study of wound infections following major head and neck cancer surgery was undertaken to define a rational approach to trials of antibiotic prophylaxis and initial therapy of these infections. preoperative aerobic cultures were taken from the planned site of skin incision and from the oropharynx. both aerobic and anaerobic cultures were obtained from all wound infections. patients receiving prophylactic antibiotics were excluded from the study. the data indicate that preoperative c ... | 1977 | 329532 |
| [the growth curves of enterobacteriaceae and the estimation of l-forms under the influence of cephalosporin antibiotics]. | the antibacterial effectiveness of cephalothin, cefazolin, cephacetrile and cephradine was investigated on growing cultures of klebsiella pneumoniae recording the growth curves with a biophotometer. thereby cefazolin has a greater antibacterial activity than the other cephalosporins. by raising the dose to 100 microgram/ml after initial bactericidal effect a second growth phase was observed. phase microscopic observation showed that this was caused by an increasing number of l-forms. this phenom ... | 1977 | 329618 |
| novel r-plasmid-mediated beta-lactamase from klebsiella aerogenes. | a novel beta-lactamase with a broad spectrum of activity against penicillins and cephalosporins has been detected in a strain of klebsiella aerogenes. its synthesis is mediated by an r-plasmid of molecular weight 64 x 10(6). | 1977 | 329756 |
| [cyclodextrin glucanotransferase from klebsiella pneumoniae. 2. significance of the enzyme for the metabolism of cyclodextrins by klebsiella pneumoniae m 5 al (author's transl)]. | | 1977 | 329783 |
| microbiological relevance and clinical potential of ampicillin-cloxacillin synergism. | recent demonstration "in vitro" that combinations of ampicillin and cloxacillin, using concentrations at which neither were previously effective, will kill certain resistant gram negative bacteria, has important clinical potential. we therefore studied 50 ampicillin resistant gram negative rods cultured from septicemic patients for synergism. 7 of 23 e. coli strains with a minimal bactericidal concentration (mbc) of 128 microgram/ml were killed by combinations containing 4-32 microgram/ml ampici ... | 1977 | 329905 |
| state of the gal-transducing hybrid phage lambda ci857nin5h424gal+ genome in klebsiella pneumoniae m5a1. | | 1977 | 330392 |
| esculin hydrolysis by enterobacteriaceae. | literature reports disagree concerning esculin hydrolysis in the family enterobacteriaceae. a total of 2,490 strains of the family were investigated for esculin hydrolysis by two methods, the esculin spot test and the pathotec incubation strip, which measures constitutive enzyme, and five growth-supporting methods, which determine both constitutive and inducible enzymes. the five growth-supporting media studied were: vaughn-levine, the standard esculin hydrolysis medium (p. r. edwards and w. h. ... | 1977 | 330558 |
| relative enterotoxigenicity of coliform bacteria. | the enterotoxigenicity of 12 strains of coliform bacteria (enterobacter cloacae, klebsiella pneumoniae, and escherichia coli) isolated from the gastrointestinal (gi) tract of persons with either acute diarrhea or tropical sprue and of 13 strains of the same species isolated from urine (gu) cultures was determined. fractions of heat-labile (lt) and heat-stable (st) toxins of each strain were separated by ultrafiltration, and the effect of graded concentrations (range, 100 microgram-10 pg/ml) on w ... | 1977 | 330767 |
| epidemiology of nosocomial infection due to gram-negative bacilli: aspects relevant to development and use of vaccines. | | 1977 | 330774 |
| cross-reactive antigens: their potential for immunization-induced immunity to gram-negative bacteria. | the involvement of multiple species and serologic types in gram-negative bacteremia prompted evaluation of immunization with shared, cross-reactive antigens of gram negative bacilli. active and passive immunization with re chemotype mutants of salmonella minnesota afforded significant protection against heterologous gram-negative bacilli and were considerably more effective than immunization with smooth s. minnesota or its ra, rb, rc, rd1 and rd2 mutants. since the lipopolysaccharide of the re m ... | 1977 | 330775 |
| alkaloids of thalictrum. xxi. isolation and characterization of alkaloids from the roots of thalictrum podocarpum. | thirteen alkaloids, hernandezine, thalidezine, n-desmethylthalidezine, isothalidezine, thalistyline, thalistyline methodiiodide, n-desemethylthalistyline, berberine, columbamine, jatrorrhizine, palmatine, thalifendine, magnoflorine and the artifact, 8-trichloromethyldihydroberberine were isolated from the roots of thalictrum podocarpum humb. in addition, oxyberberine and thaliglucinone were obtained in very minor amounts and identified by tlc. of these compounds, n-desmethylthalidezine and isoth ... | 1977 | 331006 |
| recombinant plasmid that carries part of the nitrogen fixation (nif) gene cluster of klebsiella pneumoniae. | we have cloned fragments of the klebsiella pneumoniae genome that carry part of the his operon and part of the nitrogen fixation (nif) gene cluster on the amplifiable plasmid pmb9. one particular plasmid, pcra37, complements mutations in the hisd, nifb, and niff loci. the physical map of pcra37 as determined by restriction enzyme analysis correlates with the genetic map of the his-nif region as determined previously by phage p1-mediated cotransductional analysis. | 1977 | 331321 |
| bacteremia after renal transplantation. | of 291 patients who received renal allotransplants in 1964-74, 94 patients developed 121 bacteremic episodes within the first 6 months after transplantation. the death rate was 38%. the bacteremic episodes occurred mainly during the first 2-3 months after transplantation. in 65% of the episodes bacteremia was secondary to urinary tract infections. the bacteria fourn were most often gram-negative rods such as escherichia coli, klebsiella and proteus. predisposing factors were ureteral complicatio ... | 1977 | 331458 |
| in vitro synergy of cefamandole-tobramycin combinations. | | 1977 | 331694 |
| [demonstration of antibody-coated bacteria in the urine of pregnant women]. | urinary sediments of 64 pregnant women with significant bacteriuria which has been demonstrated several times before, have been investigated for the presence of antibody coated bacteria. antibody titers were determined in the corresponding serum samples in addition and the results were compared with the clinical diagnosis. in 50% of pregnant women with pyelonephritis antibody coated bacteria could be demonstrated, while 60% of them had elevated serum titers. taking into account both results in 8 ... | 1977 | 331754 |
| mercury resistance and r plasmids in clinical isolates of kebsiella pneumoniae. | the mercury and antibiotic resistance of 155 clinical isolates of klebsiella pneumoniae (k. pneumoniae) was determined. resistance to mercury tested was found in 64.5% of the total isolates. the frequencies of resistance to sm, tc, cp, km and gm were 65.8%, 49.0%, 48.4%, 37.4% and 3.9%, respectively. and we selected 94 mercury and drug resistant strains, and they were tested for their ability to transfer the resistance to the mercury and drug sensitive recipients of e. coli k12 ml 1410-nx and e. ... | 1977 | 331756 |
| the clinical usefulness of an ongoing bacteremia surveillance program. | surveillance of bacteremic patients within one week of the detection of a positive blood culture provides information of great clinical utility for relatively little effort. in this report one year's experience is reviewed to illustrate that an individual hospital's experience with bacteremia may diverge from national norms. such reviews may also detect recurring errors in medical judgment which may be corrected through proper education. | 1977 | 331951 |
| bacteriostatic inhibition of klebsiella pneumoniae by three human transferrins. | three human transferrin variants verified by rivanol precipitation were separated using vertical block polyacrylamide gel electrophoresis followed by elution convection. a bacteriostatic inhibition measurement in vitro of the three transferrins was made. the data indicate that human transferrin variants exhibit different degrees of inhibition on klebsiella pneumoniae. | 1977 | 332055 |
| effect of dimethyl sulphoxide on the expression of nitrogen fixation in bacteria. | storage in dimethyl sulphoxide (dmso) of escherichia coli k12 hybrids carrying nif+ genes from klebsiella pneumoniae can result in selection of a defective nitrogen-fixing phenotype. similar results are obtained with e. coli k12 hybrids containing the nitrogen-fixing capacity from rhizobium trifolii. dmso appears to affect particular inner membrane proteins associated with energy metabolism in e. coli k12 and four chromosomal regions (chld, chlg, his and unc) are associated with resistance to dm ... | 1977 | 332135 |
| isolation of poly-alpha-l-guluronate lyase from klebsiella aerogenes. | the bacterium klebsiella aerogenes type 25 produces an extracellular alginolyase which has been partly purified. the enzyme is specific for the alpha-l-guluronosyl linkages in whole alginate and fractions therefrom. the end products of its action on polyguluronic acid blocks are mainly the unsaturated di- and tri-saccharides, with a smaller proportion of the homologous tetrasaccharide. some general properties of the enzyme are reported. | 1977 | 332364 |
| detection of bacterial infection of the pancreatic ducts in patients with pancreatitis and pancreatic cancer during endoscopic cannulation of the pancreatic duct. | bacterial infections of the pancreas and bacteremia may occur during episodes of pancreatitis. detection of bacterial infections of the pancreas in the past has required laparotomy. the present study was undertaken to determine whether bacterial infection of the pancreas occurred during nonsuppurative pancreatitis. during endoscopic cannulation of the main pancreatic duct, secretin was administered intravenously and pancreatic juice aspirated from within the duct was cultured. bacterial infectio ... | 1977 | 332575 |
| immunological interrelationships between cholera toxin and the heat-labile and heat-stable enterotoxins of coliform bacteria. | cholera toxin (ct) and the heat-labile (lt) toxin of escherichia coli are known to share antigenic properties. the present study examined the immunological relationship of ct and the lt and heat-stable (st) toxins of e. coli, klebsiella pneumoniae, and enterobacter cloacae. the neutralizing capacity of equine ct antiserum and of antiserum raised in rabbits to the lt toxin of the three species of coliform bacteria was evaluated by determining their capacity to inhibit the action of purified ct an ... | 1977 | 332637 |
| experience with amikacin and colistin in an outbreak of infection by resistant klebsiella aerogenes. | | 1977 | 332682 |
| assessment of technique for rapid detection of escherichia coli and proteus species in urine by head-space gas-liquid chromatography. | a test depending on the production of ethanol by escherichia coli from lactose and dimethyl disulfide by proteus spp. from methionine in the early exponential phase of growth and the detection of these products by head-space gas-liquid chromatography has been applied to 75 specimens of urine selected to provide the most stringent trial of the test. the test was found to be rapid and reliable for the commonest findings in the microbiological examination of urine. in 3 to 4 h it detected "signific ... | 1977 | 332707 |
| rapid deoxyribonuclease test with methyl green. | a 4-h deoxyribonuclease test using methyl green to differentiate serratia from other enterobacteriaceae was developed. the tests agreed 100% with an overnight plate test for 100 serratia, 83 enterobacter, and 6 klebsiella species. | 1977 | 332714 |
| synthesis and antibacterial activity of some derivatives of tolypomycinone. relationship between structure and activity in ansamycins. | 3-aminotolypomycinoes and 3,16-diamino-16,17-dihydrotolypomycinones are formed by the addition of primary and secondary amines to tolypomycinone, obtained by mild hydrolysis of the antibiotic tolypomycin y.3-amino-16,17-dihydrotolypomycinones are formed by the addition of primary and secondary amines to 16,17-dihydrotolypomycinone. in vitro microbiological tests showed high antibacterial activity in compounds obtained by the addition of primary amines, which must be unbranched in the alpha posit ... | 1977 | 333113 |
| an empiric approach to pneumonia. | | 1977 | 333220 |
| explusion of salibi-clamp from the internal carotied artery in the neck by infection. | a left infraclinoid carotid aneurysm (3 x 2-5 x 2 cm) in a 60-year old woman presented with total ophthalmoplegia and trigeminal symptoms. a staged internal carotied occlusion in the neck was performed using a salibi-clamp. a chronic infectious fistula developed (klebsiella aerogenes). when superficial debridement failed, operative removal of the salibi-clamp was planned seven months after its insertion. the clamp was found subcutaneously without any relation to the internal carotid artery and w ... | 1977 | 333303 |
| the role of plasma cells in scleroma. electron-microscopic study. | three cases of rhinoscleroma in the granulomatous stage were examined under the electrom microscope. the rough endoplasmic reticulum of plasma cells in scleroma displayed cisternal dilatations forming russell bodies and cytoplasmic vacuolations containing viable and degenerated bacilli forming mikulicz cells. thus, they play a double role, producing antibodies and providing protection for the intracellular bacilli. new ultrastructural findings of klebsiella rhinoscleromatic bacilli were describe ... | 1977 | 333340 |
| maternal septicaemia and death after circumcision. | | 1977 | 333668 |
| [taxonomic study of enterobacteria belonging or related to the genus klebsiella (author's transl)]. | this work studies the classification, by numerical procedure, of 122 strains belonging or related to the genus klebsiella. four classes can be defined: j (= k. pneumoniae: 29 strains for the most isolated from human faeces), k (related to k. pneumoniae and to k. oxytoca: 24 strains isolated from water), l (related to k. pneumoniae although, tetrathionate-reductase+, m-hydrobenzoate+: 37 strains isolated from water or soil), m (= k. oxytoca: 22 strains for the most isolated from water). the impor ... | 1977 | 334015 |
| interaction between the nitrate respiratory system of escherichia coli k12 and the nitrogen fixation genes of klebsiella pneumoniae. | | 1977 | 334174 |
| affinity chromatography od klebsiella arylsulfatase on tyrosyl-hexamethylenediamine-beta-1,3-glucan and immunoadsorbent. | a simple and convenient method for preparation of a highly purified arylsulfatase (ec 3.1.6.1) from klebsiella aerogenes has been developed. specificity of purification was achieved by using affinity chromatography on a tyrosyl-hexamethylenediamino-beta-1,3-glucan or on a solid phase immunoadsorbent. by using affinity chromatography a homogeneous enzyme was obtained with high yield. it is also proposed that the beads of curdlan type polysaccharide consisting of beta-1,3-glucan can be used as a g ... | 1977 | 334267 |
| health effects of short-term inhalation of nitrogen dioxide and ozone mixtures. | | 1977 | 334528 |
| acriflavine violet red bile agar for the isolation of klebsiella. | a medium for the isolation and detection of klebsiella is described. it contains 0.06% acriflavine in violet red bile agar (difco). klebsiella appeared as 5 to 7 mm mucoid (24 h at 37 degrees c) golden-yellow colonies. pseudomonas aeruginosa and enterobacter spp. appeared as small, brown to dark brown colonies. escherichia coli as well as many other gram negative organisms and gram positive organisms did not grow on this medium. | 1977 | 334697 |
| intramammary infections in a dairy herd with a low incidence of streptococcus agalactiae and staphylococcus aureus infections. | in a dairy herd with a low incidence of intrammary infections due to streptococcus agalactiae and staphylococcus aureus, clinical mastitis remained a serious problem despite good control of nonclinical mastitis through postmilking teat disinfection and antibiotic therapy of known infected quarters at the end of lactation. during the 2-year study, the incidence of clinical mastitis was 0.88 cases/cow-year; 32.2% were caused by streptococcal species other than str agalactiae and 33.5% by gram-nega ... | 1977 | 334713 |
| reactivity and inhibitor potential of hydroxycitrate isomers with citrate synthase, citrate lyase, and atp citrate lyase. | the four isomers of hydroxycitrate have been tested as substrates and inhibitors for citrate synthase, citrate lyase, and atp citrate lyase. none of the isomers served as a substrate for citrate synthase and they were moderate to weak inhibitors of this reaction. of the four isomers, only (pncit)-(2s)-2-hydroxycitrate did not serve as a substrate for citrate lyase while (pncit)-(4s)-4-hydroxycitrate was the only isomer which did not serve as a substrate for atp citrate lyase. no consistent patte ... | 1977 | 334761 |
| enzymatic degradation of polygalacturonic acid by yersinia and klebsiella species in relation to clinical laboratory procedures. | as scored by several specified plating procedures, clinical and environmental strains of yersinia enterocolitica, yersinia pseudotuberculosis, and klebsiella pneumoniae "oxytocum" showed detectable, albeit generally weak, ability to digest polygalacturonic (pectic) acid. none of these bacterial strains had the vigorous and rapid pectolytic activity on these polygalacturonic acid-containing media that is typical of soft-rot erwinia species, although some of the oxytocum strains came fairly close. ... | 1977 | 334794 |
| preprototype of an automated microbial detection and identification system: a developmental investigation. | the automicrobic system is an automated, computerized instrument that uses highly selective media and an optical system for detection, enumeration, and identification of bacteria and some yeasts in 13 h. a preprototype instrument (automicrobic system-1) and its urine culture kit (identi-pak), developed for the detection, enumeration, and identification of eight species or groups of bacteria and of candida species and torulopsis glabrata in urine specimens, was evaluated during its development. a ... | 1977 | 334797 |