| [sepsis induced by hafnia alvei in a kidney transplant patient]. | | 2008 | 18662166 |
| [intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees]. | dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ... | 2008 | 18683661 |
| re: hafnia alvei infection of the deep palmar space. | | 2008 | 18687848 |
| biodiversity of the microbial community in a spanish farmhouse cheese as revealed by culture-dependent and culture-independent methods. | the microbial diversity within alberquilla cheese, made from a spontaneously fermented mixture of raw goats' and sheep's milk in the alpujarra mountains (granada, south-east spain), has been studied by the classical culturing method and also by molecular analysis of community dna. a collection of 206 isolates was obtained from the cheese on different selective/differential media, which were then re-grouped to 52 after randomly amplified polymorphic dna (rapd)-pcr analyses. isolates on man-rogosa ... | 2008 | 18692931 |
| graft versus host disease-related hafnia alvei colonization and probable infection. | we describe the case of a graft versus host disease (gvhd) patient, in whom hafnia alvei was cultured as a single organism, and at high bacterial counts from stool samples, from the onset of the disease until its resolution. this case is a further example of the contentious role of this species in causing human intestinal disease. furthermore, it focuses on enteric damage by gvhd as a risk factor for acquiring h. alvei colonization, and probably infection. | 2008 | 18719190 |
| diversity of auxin-producing bacteria associated to pseudomonas savastanoi -induced olive knots. | forty three strains were isolated from knots induced by pseudomonas savastanoi in different olive cultivars. all the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (iaa). amplification of the intergenic transcribed spacers (its) between 16s and 23s rdna genes, allowed the clustering of the isolates into seven distinct groups. all isolates from its group 1 were positive to the pseudomonas savastanoi pv. savastanoi specific iaa l gene as s ... | 2008 | 18759227 |
| impact on disinfection efficiency of cell load and of planktonic/adherent/detached state: case of hafnia alvei inactivation by plasma activated water. | this paper describes the effects of initial microbial concentration and planktonic/adherent/detached states on the efficiency of plasma-activated water. this disinfecting solution was obtained by treating distilled water with an atmospheric pressure plasma produced by gliding electric discharges in humid air. the inactivation kinetics of planktonic cells of hafnia alvei (selected as a bacterial model) were found to be of the first order. they were influenced by the initial microbial concentratio ... | 2008 | 18769918 |
| invasion and intracellular survival of hafnia alvei strains in human epithelial cells. | the aim of this study was to investigate the invasion and intracellular survival of different hafnia alvei strains in hela cells. | 2008 | 18795976 |
| structure of the glycerol phosphate-containing o-specific polysaccharide and serological studies on the lipopolysaccharides of citrobacter werkmanii pcm 1548 and pcm 1549 (serogroup o14). | the o-specific polysaccharide was obtained by mild acid hydrolysis of the lipopolysaccharide of citrobacter werkmanii pcm 1548 and pcm 1549 (serogroup o14) and found to contain d-glucose, d-glucosamine and glycerol-1-phosphate in molar ratios 2 : 2 : 1. based on methylation analysis and 1h and 13c nuclear magnetic resonance spectroscopy data, it was established that the o-specific polysaccharides from both strains have the identical branched tetrasaccharide repeating unit with 3,6-disubstituted ... | 2008 | 18811720 |
| immunochemical studies of the lipopolysaccharides of hafnia alvei pcm 1219 and other strains with the o-antigens containing d-glucose 1-phosphate and 2-deoxy-2-[(r)-3-hydroxybutyramido]-d-glucose. | hafnia alveiis the only species of the genus hafnia, which belongs to the family of enterobacteriaceae. these gram-negative bacteria are commonly distributed in the natural environment and are often the cause of human opportunistic infections. their lipopolysaccharides (lpss) are important surface antigens which are responsible for the serological specificity and numerous cross-reactions with other enterobacterial genera. so far, 29 different o-polysaccharide (ops, o-antigen) structures in hafni ... | 2008 | 18836891 |
| [the analysis of the bacterial and fungal flora in maxillary sinuses in patients operated due to fess method]. | the aim of this work was to analyse the bacterial and fungal flora in maxillary sinuses in patients operated due to the fess method. | 2008 | 18837224 |
| escherichia albertii and hafnia alvei are candidate enteric pathogens with divergent effects on intercellular tight junctions. | attaching-effacing lesion-inducing escherichia albertii and the related, but non-attaching-effacing organism, hafnia alvei, are both implicated as enteric pathogens in humans. however, effects of these bacteria on epithelial cells are not well-characterized. related enteropathogens, including enterohemorrhagic escherichia coli o157:h7, decrease epithelial barrier function by disrupting intercellular tight junctions in polarized epithelia. therefore, this study assessed epithelial barrier functio ... | 2008 | 18930803 |
| two kdo-heptose regions identified in hafnia alvei 32 lipopolysaccharide: the complete core structure and serological screening of different hafnia o serotypes. | hafnia alvei, a gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. various 3-deoxy-d-manno-oct-2-ulosonic acid (kdo)-containing fragments different from known structures of core oligosaccharides were previously found among fractions obtained by mild acid hydrolysis of some h. alvei lipopolysaccharides (lpss). however, the positions of these segments in the lps structure were not known. analysis of de- ... | 2009 | 19011031 |
| temperature influences the expression of fimbriae and flagella in hafnia alvei strains: an immunofluorescence study. | hafnia alvei, a gram negative bacillus related to the enterobacteriaceae family, is considered an opportunistic pathogen of several animal species and humans. in this communication, we describe fimbrial-like structures from different strains of h. alvei that cannot be easily ascribed to any of the previously reported fimbrial types in this species (type i or type iii). polymerase chain reaction (pcr) and immunofluorescence assays were carried out to study fimbriae and flagella in h. alvei strain ... | 2009 | 19011836 |
| normalization of leaky gut in chronic fatigue syndrome (cfs) is accompanied by a clinical improvement: effects of age, duration of illness and the translocation of lps from gram-negative bacteria. | there is now evidence that an increased translocation of lps from gram negative bacteria with subsequent gut-derived inflammation, i.e. induction of systemic inflammation and oxidative & nitrosative stress (io&ns), is a new pathway in chronic fatigue syndrome (cfs). | 2008 | 19112401 |
| isolation of colistin-resistant hafnia alvei. | | 2009 | 19141752 |
| microbial inactivation using plasma-activated water obtained by gliding electric discharges. | to evaluate the microbial disinfection efficacy of a plasmachemical solution obtained by the activation of water with gliding electric discharges. | 2009 | 19170858 |
| molecular identification of mesophilic and psychrotrophic bacteria from raw cow's milk. | the aim of this study was to use molecular techniques to assess the microbiota of eight raw cow's milk samples at biotype and species level. sixty-six isolates from raw milk samples were screened by randomly amplified polymorphic dna-pcr (rapd-pcr) biotyping and representative strains of rapd-pcr profiles were identified by 16s rrna gene sequencing. pseudomonas spp. were the most commonly occurring contaminants along with enterobacteriaceae such as hafnia alvei, serratia marcescens and citrobact ... | 2009 | 19171267 |
| isolation of hafnia species from kimchi. | eight commercially packaged kimchi products were examined over 15 days of storage at 4 degrees to evaluate the occurrence of hafnia alvei (h. alvei). additionally, 7 saeujeot products, as a possible ingredient source, were analyzed to examine the bacteria's origin. over the storage period, kimchi samples had decreasing ph levels, which stabilized at ph 4.2; acidity increased to 0.9+/-0.1%. lactose-nonfermenting bacteria, which h. alvei belongs to, gradually reduced in numbers over the kimchi sto ... | 2009 | 19190412 |
| [taxonomic characteristics and physiological properties of microorganisms from the gut of pike (esox lucius)]. | the taxonomic composition and distribution of microorganisms differing in the degree of association with the intestinal mucosa of the pike (lucius lucius) has been studied. microorgansism of the families enterobacteriaceae, aeromonadaceae, and vibrionaceae dominate in the gut microflora. numerically prevailing bacterial species are characterized by high proteolytic and amylolytic enzyme activities as well as by high persistence accounted for by antilysozyme and antihistone activities. the result ... | 2008 | 19198074 |
| comparison of pcr-based molecular markers for the characterization of proteus mirabilis clinical isolates. | proteus mirabilis is one of the most important pathogens associated with complicated urinary tract infections (acute pyelonephritis, bladder infections, kidney stones) and bacteremia, affecting patients with anatomical abnormalities, immunodeficiency, and long-term urinary catheterization. for epidemiological purposes, various molecular typing methods, such as pulse-field gel electrophoresis (pfge) or ribotyping, have been developed for this pathogen. however, these methods are labor intensive a ... | 2008 | 19219283 |
| polyphasic study of microbial communities of two spanish farmhouse goats' milk cheeses from sierra de aracena. | the microbial communities present in 2 different types of farmhouse goats' milk cheese from the aracena mountains (southwest spain), quesailla arochena (hard cheese) and torta arochena (soft cheese), have been studied using both culture-dependent and culture-independent techniques. all bacterial isolates were clustered by using randomly amplified polymorphic dna (rapd) and identified by 16s rrna gene sequencing, species-specific pcr and multiplex pcr. thus a total of 26 different species were id ... | 2009 | 19269572 |
| [aberrant gallstones--extraordinary complications of laparoscopic cholecystectomy]. | about 0.2 % of patients undergoing laparoscopic cholecystetomy will suffer from complications caused by lost gallstones. diagnostic and therapeutic measures are correlated to the symptoms. at different locations, abscesses can arise, which can be treated by direct access through the -abdominal wall, laparotomy or laparoscopy. two cases are presented with the especially grave sequels of subphrenic abscess, infiltration of the thoracic wall, and pleural empyema, which -respectively needed several ... | 2009 | 19382051 |
| clostridium perfringens liver abscess after pancreatic resection. | liver abscess is a rare complication after pancreatectomy. clostridium perfingens is a rare cause of intrahepatic infections. | 2009 | 19388837 |
| effects of proteus vulgaris growth on the establishment of a cheese microbial community and on the production of volatile aroma compounds in a model cheese. | to investigate the impact of proteus vulgaris growth on a multispecies ecosystem and on volatile aroma compound production during cheese ripening. | 2009 | 19426267 |
| gangrenous cholecystitis caused by hafnia alvei: a case report and review of literature. | | 2009 | 19497550 |
| phenotypic characterization of human pathogenic bacteria in fish from the coastal waters of south west cameroon: public health implications. | increasing economic and recreational opportunities, attractive scenery and a perception of a better quality of life are luring people to the coast. unfortunately, these activities together with the commensurate increase in population in the area inevitably result in pollution of coastal waters with excessive microorganisms and other pollutants. microbial pollutants not only contaminate the coastal water but also aquatic food sources, thus posing a health risk to consumers. fish is a major source ... | 2009 | 19658320 |
| gram-negative bacteria associated with brewery yeasts: reclassification of obesumbacterium proteus biogroup 2 as shimwellia pseudoproteus gen. nov., sp. nov., and transfer of escherichia blattae to shimwellia blattae comb. nov. | phylogenetic analyses of type and reference strains of obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16s rrna gene sequences and partial sequences of four protein-coding genes (fusa, leus, pyrg and rpob). both approaches revealed that o. proteus biogroup 1 strains were closely related to hafnia alvei. biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included escherichia blattae. phenotypic tests were ... | 2010 | 19661513 |
| structural analysis of the lipid a isolated from hafnia alvei 32 and pcm 1192 lipopolysaccharides. | hafnia alvei, a gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. the majority of clinical symptoms of diseases caused by this bacterium have a lipopolysaccharide (lps, endotoxin)-related origin. the lipid a structure affects the biological activity of endotoxins predominantly. thus, the structure of h. alvei lipid a was analyzed for the first time. the major form, asymmetrically hexa-acylated lipid ... | 2010 | 19706748 |
| hafnia paralvei sp. nov., formerly known as hafnia alvei hybridization group 2. | it has been shown previously, based largely on dna-dna hybridizations and partial 16s rrna gene sequencing, that hafnia alvei is genotypically heterogeneous and consists of at least two dna hybridization groups (hgs). in the present study, the taxonomic status of h. alvei hgs 1 and 2 was reassessed. a panel of 24 reference strains and isolates previously assigned to one of the two hgs in h. alvei was subjected to (gtg)5-pcr fingerprinting; this resulted in the delineation of two (gtg)5-pcr clust ... | 2010 | 19734282 |
| [hafnia alvei]. | | 2009 | 19802404 |
| technology-induced selection towards the spoilage microbiota of artisan-type cooked ham packed under modified atmosphere. | the microbiota associated with a highly-perishable belgian artisan-type cooked ham was analyzed through plating and (gtg)(5)-fingerprinting of isolates throughout its processing chain. the raw tumbled meat was characterized by the presence of a versatile microbiota around 4.8 log(cfu g(-1)), consisting of lactic acid bacteria, staphylococci, brochothrix thermosphacta, gram-negative bacteria, and yeasts. pasteurisation of the ham logs reduced bacterial counts below 2 log(cfu g(-1)) and subsequent ... | 2010 | 19913696 |
| novel o-antigen of hafnia alvei pcm 1195 lipopolysaccharide with a teichoic acid-like structure. | the lipopolysaccharide (lps) of hafnia alvei strain pcm 1195 was obtained by the hot phenol/water method. the o-specific polysaccharide was released by mild acidic hydrolysis and isolated by gel filtration. the structure of the o-specific polysaccharide was investigated by (1)h, (13)c, and (31)p nmr spectroscopy, maldi-tof ms, and gc-ms, accompanied by monosaccharide and methylation analysis. it was concluded that the o-specific polysaccharide is composed of a hexasaccharide repeating units inte ... | 2010 | 20003963 |
| inhibition of the early stage of salmonella enterica serovar enteritidis biofilm development on stainless steel by cell-free supernatant of a hafnia alvei culture. | compounds present in hafnia alvei cell-free culture supernatant cumulatively negatively influence the early stage of biofilm development by salmonella enterica serovar enteritidis on stainless steel while they also reduce the overall metabolic activity of s. enteritidis planktonic cells. although acylhomoserine lactones (ahls) were detected among these compounds, the use of several synthetic ahls was not able to affect the initial stage of biofilm formation by this pathogen. | 2010 | 20097823 |
| histamine, cadaverine, and putrescine produced in vitro by enterobacteriaceae and pseudomonadaceae isolated from spinach. | a total of 364 bacterial isolates, obtained from spinach leaves, were assayed in a decarboxylase broth containing histidine, lysine, and ornithine to check their ability to produce biogenic amines, and then quantified by high-performance liquid chromatography. among these isolates, 240 formed cadaverine, 208 formed putrescine, and 196 formed histamine, in widely varying amounts. they frequently produced more than one biogenic amine. klebsiella pneumoniae subsp. pneumoniae and morganella morganii ... | 2010 | 20132689 |
| biofilm formation and acyl homoserine lactone production in hafnia alvei isolated from raw milk. | the objective of this study was to detect the presence of acyl homoserine lactones (ahls), signal molecules of the quorum sensing system in biofilm formed by hafnia alvei strains. it also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. ahls were assayed using well diffusion techniques, thin layer chromatography (tlc) and detection directly in biofilm with biomonitors. the extracts obtained from planktonic and sessile cell of h. alvei induced at least two of thre ... | 2010 | 20140298 |
| [report of four clinical cases of hafnia alvei bacteremia in a pediatric cardiac surgery unit]. | the gram-negative bacillus hafnia alvei is the only species of the genus hafnia, family enterobacteriaceae. it occasionally behaves as an opportunistic pathogen in humans, causing intestinal and respiratory infection and sepsis. it rarely causes bacteremia, usually of unknown focus. | 2010 | 20140313 |
| an unusual organism for pd-related peritonitis: hafnia alvei. | | 2010 | 20200377 |
| production of biogenic amines "in vitro" in relation to the growth phase by enterobacteriaceae species isolated from traditional sausages. | histidine, lysine, ornithine and tyrosine decarboxylase activities were tested in 79 strains of enterobacteriaceae (41 of hafnia alvei, 17 of serratia liquefaciens, 5 of enterobacter cloacae, 4 of citrobacter braakii, 2 of proteus vulgaris, 2 of proteus mirabilis, 2 of providencia stuartii, 2 of klebsiella terrigena, 1 of rahnella aquatilis, 1 of salmonella arizonae, 1 of citrobacter youngae and 1 of escherichia coli) isolated from botillo, a spanish traditional sausage. in general, the strains ... | 2010 | 20673614 |
| selective testing of ß-galactosidase activity in the laboratory identification of salmonella and shigella species. | salmonella and shigella species are pathogens of great medical and public health importance. however, laboratory identification of these organisms is time consuming. using current standard laboratory algorithms, the vast majority of organisms submitted for serological typing with salmonella-specific and shigella-specific antisera are not clinically significant. | 2010 | 20924038 |
| improved selective and differential medium for isolation of escherichia coli o157:h7. | gmac, a modified version of sorbitol macconkey medium (smac), was produced with a reduced quantity of selective agents and incorporated gentiobiose. gmac supported a higher recovery rate of heat- or acid-injured escherichia coli o157:h7 cells than smac with cefixime and tellurite (ct-smac), while differentiating e. coli o157:h7 from sorbitol-nonfermenting hafnia alvei. | 2010 | 20980579 |
| characterization of the enterobacteriaceae community that developed during storage of minced beef under aerobic or modified atmosphere packaging conditions. | the whole cell protein and macrorestriction analysis of dna of enterobacteriaceae isolates recovered from minced beef stored at 0, 5, 10 and 15 °c aerobically and under modified atmosphere packaging consisting of 40% co(2)-30% o(2)-30% n(2) in the presence (map+) and absence (map-) of oregano essential oil were studied. sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page) profiles obtained from whole cell protein analysis of the enterobacteriaceae isolates revealed seven groups. ... | 2010 | 21216482 |
| hafnia alvei pyelonephritis in a renal transplant recipient: case report and review of an under-recognized nosocomial pathogen. | a.p. cardile, d. forbes, v. cirigliano, b. stout, n.p. das, g. hsue. hafnia alvei pyelonephritis in a renal transplant recipient: case report and review of an under-recognized nosocomial pathogen. transpl infect dis 2011: 13: 407-410. all rights reserved abstract: we describe the first case to our knowledge of hafnia alvei pyelonephritis in a renal transplant recipient. clinicians should consider this under-recognized pathogen when clinically evaluating immunosuppressed patients with a history o ... | 2011 | 21299775 |
| a case of bacteremia caused by hafnia paralvei. | the genus hafnia, a member of the enterobacteriaceae, is widespread in nature and rarely causes human infection. we describe a case of an 85-year-old japanese man hospitalized consequent to suspected cholecystitis, in which hafnia sp. was recovered from the blood culture concomitantly with enterococcus faecalis. sequencing of the 16s ribosomal rna gene sequence and phenotyping with id 32 e revealed that the recovered hafnia sp. was considered to be hafnia alvei genomosp. 2 (atcc 29927), recently ... | 2011 | 21553084 |
| clinical and laboratory diagnostic characteristics and cytotoxigenic potential of hafnia alvei and hafnia paralvei strains. | a collection of 68 strains of hafnia previously identified to species by 16s rrna gene sequencing were investigated for simple phenotypic properties that could aid in their recognition in the clinical laboratory. four tests including malonate utilization, fermentation of salicin and d-arabinose, and expression of ß-glucosidase activity correctly assigned each strain to either h. alvei or h. paralvei. antibiotic susceptibility profiles for 35 hafnia alvei and hafnia paralvei isolates were generat ... | 2011 | 21795516 |
| yokenella regensburgei in an immunocompromised host: a case report and review of the literature. | yokenella regensburgei belongs to the enterobacteriaceae and shares some biochemical characteristics with hafnia alvei. a few case reports have suggested that it is an opportunistic pathogen, but there is no strong evidence to support its clinical importance. until recently, it was difficult to accurately differentiate between y. regensburgei and h. alvei by use of routine identification techniques. here, we present a case of soft tissue infection and bacteremia caused by y. regensburgei, which ... | 2011 | 21706224 |
| Ecological and aromatic impact of two Gram-negative bacteria (Psychrobacter celer and Hafnia alvei) inoculated as part of the whole microbial community of an experimental smear soft cheese. | The impact of the growth of two Gram-negative bacteria, Psychrobacter celer and Hafnia alvei, inoculated at 10(2) and 10(6)cfu/g, on the dynamics of a multispecies community as well as on volatile aroma compound production during cheese ripening was investigated. Results showed that P. celer was able to successfully implant itself in cheese, regardless of its inoculation level. However, when it was inoculated at a high level, the bacterial biodiversity was drastically lowered from day 25 to the ... | 2011 | 22177851 |
| biogenic amines produced by enterobacteriaceae isolated from meat products. | biogenic amines in ground meat and processed meat products are one of the indicators to determine the poor quality raw material. major histamine forming bacterium was escherichia coli (strain ec04 with 65.88 mg/100 ml in brain heart infusion medium), followed by the microorganisms morganella morganii (strain mm4 with 8.40 mg/100 ml and strain mm7 with 8.28 mg/100 ml) and proteus mirabilis (strain pm02 with 8.76 mg/100 ml), respectively. the highest putrescine production level was found in citrob ... | 2001 | 22062111 |
| new functional ligands for ficolin-3 among lipopolysaccharides of hafnia alvei. | ficolin-1 (m), ficolin-2 (l), ficolin-3 (h) and mannan-binding lectin (mbl) activate the complement system and have opsonic activity. the specificity of ficolin-3 is poorly characterized and currently limited to a few ligands only. we present new specific targets for human ficolin-3, identified among lipopolysaccharides (lpss, endotoxin) of hafnia alvei. the interaction was restricted to lpss of four strains: 23, polish collection of microorganisms (pcm) 1200, pcm 1203 and pcm 1205 and limited t ... | 2011 | 21890891 |
| identification of the methyl phosphate substituent at the non-reducing terminal mannose residue of the o-specific polysaccharides of klebsiella pneumoniae o3, hafnia alvei pcm 1223 and escherichia coli o9/o9a lps. | o-specific polysaccharides of gram-negative bacteria are synthesized by two different mechanisms: polymerization of the pre-formed o-repeating unit or sequential addition of the monosaccharides to the growing polysaccharide chain. in the second case, growth of the polymer can be further subdivided into two groups depending on the presence or absence of a special monosaccharide or non-sugar substituent that terminates the glycan. a family of polymannose o-polysaccharides provides prototypes for t ... | 2011 | 22169179 |
| necrotizing fasciitis following endoscopic harvesting of the greater saphenous vein for coronary artery bypass graft. | the greater saphenous vein (gsv) remains the most commonly harvested conduit for revascularization in coronary artery bypass grafting (cabg). published literature shows that minimally invasive vein harvesting techniques have a significantly lower incidence of wound infection rates than conventional open vein harvesting techniques have. we report a case of necrotizing fasciitis, an infection with a mortality rate of 30% to 50%, after endoscopic harvesting of the greater saphenous vein to be used ... | 2011 | 21902951 |
| microbiological characteristics of precooked, vacuum-packaged uncured beef and pork. | microbiological examination of 29 packages of precooked, vacuum-packaged beef and pork products purchased at local and regional supermarkets along with 110 packages of precooked, vacuum-packaged, sliced roast beef obtained from a major meat processor did not disclose the presence of escherichia coli, coagulase-positive staphylococcus aureus, clostridium perfringens or salmonella. the effects of storage temperature and length of storage on the microflora of 110 packages of sliced roast beef were ... | 1989 | 22056107 |
| a note on escherichia coli o157:h7 serotype in turkish meat products. | 255 minced beef, 101 soudjouk and 50 uncooked hamburger samples were analyzed for the presence of escherichia coli o157:h7 serotype. m-ec and lst broths were used as selective enrichment media and smac agar was used as a selective isolation medium. a total of 3 e. coli o157 were isolated by conventional culture techniques; one from each of minced beef, uncooked hamburger and soudjouk but none were identified as the h7 serotype. for determination of selective media-growing cohabitant bacteria, 26 ... | 2000 | 22062162 |
| Identification of Yersinia ruckeri from diseased salmonid fish by Fourier transform infrared spectroscopy. | Yersinia ruckeri is the causative agent of enteric redmouth disease (ERM), which mainly affects salmonid fish. Isolates of Y. ruckeri from diseased salmonid fish were obtained over a 6-year period from eight fish farms in the State of Baden-Württemberg, Southwest Germany. The strains were characterized by biochemical methods and Fourier transform infrared spectroscopy (FT-IR) combined with artificial neural network analysis. These methods were complemented by 16S rDNA sequencing for several isol ... | 2012 | 22103737 |
| transferable integrons of gram-negative bacteria isolated from the gut of a wild boar in the buffer zone of a national park. | the aim of this study was to determine the presence of integron-bearing gram-negative bacteria in the gut of a wild boar (sus scrofa l.) shot in the buffer zone of a national park. five gram-negative strains of escherichia coli, serratia odorifera, hafnia alvei and pseudomonas sp. were isolated. four of these strains had class 2 integrase (inti2), and one harbored class 1 integrase (inti1). the integron-positive strains were multiresistant, i.e., resistant to at least three unrelated antibiotics ... | 2011 | 22661922 |
| characterisation of the spoilage microbiota in raw salmon (salmo salar) steaks stored under vacuum or modified atmosphere packaging combining conventional methods and pcr-ttge. | in order to characterise the spoilage related to microbiota of raw salmon, a combination of culture-dependent and -independent methods, including pcr-ttge, was used to analyse 3 raw salmon batches stored for 3 days at chilled temperature in modified atmosphere packaging (map) (50% co₂/50% n₂) or under vacuum. sensory evaluation, microbiological enumeration and chemical analysis were performed after 3, 7 and 10 days of storage. at the onset of spoilage, 65 bacterial isolates were picked from the ... | 2011 | 22265297 |
| impact of gram-negative bacteria in interaction with a complex microbial consortium on biogenic amine content and sensory characteristics of an uncooked pressed cheese. | the impact of gram-negative bacteria on sensory characteristics and production of volatile compounds as well as biogenic amines (ba) in the core of an uncooked pressed type model cheese was investigated in the presence of a defined complex microbial consortium. eleven strains of gram-negative bacteria, selected on the basis of their biodiversity and in vitro ba-production ability, were individually tested in a model cheese. four out of 6 strains of enterobacteriaceae (citrobacter freundii ucma 4 ... | 2011 | 22265286 |
| predominant enterobacteria on modified-atmosphere packaged meat and poultry. | enterobacteria on modified-atmosphere (ma) packaged meat (n = 54) and poultry (n = 32) products were enumerated, and 899 isolates were picked and ribotyped. for identification, 16s rrna genes of representative strains were sequenced and analyzed. altogether 54 (60%) of the samples contained enterobacteria >10(4) cfu/g. in 34% of the poultry samples, enterobacteria counts were >10(6) cfu/g suggesting that enterobacteria may contribute to spoilage of ma packaged poultry. the enterobacteria identif ... | 2012 | 23541191 |
| monitoring the succession of the biota grown on a selective medium for pseudomonads during storage of minced beef with molecular-based methods. | in the present study, the succession of the biota grown on a selective medium for pseudomonads (pseudomonas agar based medium - pab) during the storage of meat under different conditions was monitored. thus, minced beef was stored aerobically and under modified atmosphere packaging in the presence (map+) and absence (map-) of oregano essential oil at 0, 5, 10 and 15 °c. a total of 267 pure cultures were recovered from pab throughout the storage period and subjected to pcr-denaturing gradient gel ... | 2012 | 23498179 |
| evaluation of the spoilage potential of bacteria isolated from spoiled raw salmon (salmo salar) fillets stored under modified atmosphere packaging. | the spoilage potential of eight bacterial groups/species (serratia spp., hafnia alvei, brochothrix thermosphacta, carnobacterium maltaromaticum, shewanella baltica, lactococcus piscium, photobacterium phosphoreum, "other enterobacteriaceae" [containing one strain of moellerella sp., morganella sp. and pectobacterium sp.]) isolated from spoiled raw salmon fillets stored under modified atmosphere packaging (map) was evaluated by inoculation into sterile raw salmon cubes followed by storage for 12d ... | 2012 | 23290229 |
| behavior of escherichia coli o26:h11 in the presence of hafnia alvei in a model cheese ecosystem. | this study was designed to evaluate the capacity of three hafnia strains to inhibit the growth of an e. coli strain o26:h11 in an uncooked pressed model cheese, in the presence or absence of a microbial consortium added to mimic a cheese microbial community. inoculated at 2 log cfu/ml into pasteurized milk without hafnia, the e. coli o26:h11 strain reached 5 log cfu/g during cheese-making and survived at levels of 4 to 5 log cfu/g beyond 40 days. inoculated into milk at 6 log cfu/ml, all three t ... | 2012 | 23290227 |
| elaboration of highly hydrophobic polymeric surface - a potential strategy to reduce the adhesion of pathogenic bacteria? | different polymeric surfaces have been modified in order to reach a high hydrophobic character, indeed the superhydrophobicity property. for this purpose, polypropylene and polystyrene have been treated by rf or μwaves cf4 plasma with different volumes, the results were compared according to the density of injected power. the effect of pretreatment such as mechanical abrasion or plasma activation was also studied. the modified surfaces were shown as hydrophobic, or even superhydrophobic dependin ... | 2012 | 23827554 |
| cerebral perfusion pressure: a hemodynamic mechanism of mannitol and the postmannitol hemogram. | sixteen patients each received infusions of 1 g of mannitol per kg over 5 to 10 minutes, and serial determinations of intracranial pressure (icp), systemic arterial blood pressure (sabp), central venous pressure, cerebral perfusion pressure (cpp), hematocrit, hemoglobin, serum na+, k+, osmolarity, and fluid balance were carried out for 4 hours. urine output was replaced volume for volume with 5% dextrose in 0.45% nacl solution. we tested the hypothesis that patients with high (greater than or eq ... | 2010 | 3116451 |
| [literature documentation--information system]. | | 2016 | 4805592 |
| [open study of clopirac in the treatment of non articular rheumatism (author's transl)]. | | 2005 | 1084347 |
| [criteria for the selection of patients with a single heart ventricle for anatomical correction]. | the work deals with study of the functional condition of the myocardium in 34 patients with a solitary ventricle of the heart (svh). the regional function of the myocardium was disturbed in all patients; the degree of the disturbance was determined by the anatomical variant of the svh and was characterized by different distribution of the hypofunctioning segments in the ventricular myocardium. in view of that, to solve the problem of the possibility of accomplishing anatomical correction, the re ... | 2007 | 2223228 |
| the association of hla--b8 with atrophic thyroiditis. | one-hundred-and-forty-seven patients with autoimmune thyroiditis were studied with respect to hla antigens as they related to various clinical features. hla--b8 was found to be significantly increased among 59 patients with atrophic thyroiditis (57% vs. 26% for controls) but was identical to controls in 88 patients with goitrous thyroiditis (26%). no relation was found in either group between b8 and thyroid autoantibody titer or, in the case of goitrous thyroiditis, the rate of progression of th ... | 1999 | 91210 |
| resection of a massive arteriovenous malformation of the head and neck. | we report a patient with a massive arteriovenous malformation of the head and neck treated by surgical extirpation of the lesion. arteriography, hypotensive hypothermic anesthesia, and the intraoperative use of the doppler flowmeter were most helpful in this case. | 2003 | 154679 |
| effect of a mixture of cryoprotectants in attaining liquid nitrogen survival of callus cultures of a tropical plant. | | 2005 | 544179 |
| electrohydraulic lithotripsy of gallstones in humans. | | 2012 | 3366335 |
| [plastic operations on the tricuspid valve in the surgical treatment of acquired multivalvular heart diseases]. | | 2013 | 3623209 |
| urinary proline to hydroxyproline ratio varies with age. | urinary excretion of proline and hydroxyproline was studied in five groups of subjects, viz. controls, pituitary dwarfs, familial dwarfs, patients with fibrodysplasia ossificans progressive and patients with generalized scleroderma. the ratio pro/hyp in the urinary fraction precipitated with 5 parts of acetone (1 + 5 fraction) was close to one in children and youngsters in all the groups, while in adults it was higher than two. total pro/hyp does not give an accurate index of age. | 2000 | 92150 |
| history of the use and toxicity of thorotrast. | | 2004 | 387405 |
| [intraoperative monitoring of cerebral function and carotid hemodynamics during carotid endarterectomy]. | the authors investigated the hemodynamics and monitored the cerebral function to perform the carotid endarterectomy (cea) safely. the hemodynamics were investigated by measurements of carotid arterial blood flow by an electromagnetic flow meter before and after cea. and a doppler flow meter applied directly to the carotid arteries to analyze the flow parameters such as peak frequencies (pf), mean frequencies (mf), mode frequencies (mo f) and % window. we used routinely our specially designed shu ... | 2009 | 2812257 |
| [intraoperative monitoring of cerebral function and carotid hemodynamics during carotid endarterectomy]. | the authors investigated the hemodynamics and monitored the cerebral function to perform the carotid endarterectomy (cea) safely. the hemodynamics were investigated by measurements of carotid arterial blood flow by an electromagnetic flow meter before and after cea. and a doppler flow meter applied directly to the carotid arteries to analyze the flow parameters such as peak frequencies (pf), mean frequencies (mf), mode frequencies (mo f) and % window. we used routinely our specially designed shu ... | 2009 | 2812257 |
| [results of intra-arterial infusion chemotherapy in colorectal cancer patients with metastatic liver cancer]. | thirty-eight colorectal cancer patients (pts) with metastatic liver cancer who underwent intra-arterial infusion chemotherapy (iaic) at the national cancer center hospital from may 1986 to april 1988 were reviewed. excisions of metastatic liver cancer were performed in 23 pts and 15 pts had nonresectable metastatic liver cancer. catheter troubles including infections and obstructions occurred in 7 pts, and 4 pts (10.5%) were unable to receive iaic. fifteen resectable cases and 12 nonresectable c ... | 2011 | 3137879 |
| [luetic ostial mesoaortitis and myocardial infarction. a case report]. | | 2012 | 3579635 |
| [behavior of oxygen pressure in muscle (po2) in healthy subjects and in patients with intermittent claudication following standardized foot ergometric exercise]. | | 2012 | 3464140 |
| [generalized follicular and ungual mucinosis]. | | 2015 | 4642849 |
| [tasks of the sport medicine physician]. | | 2015 | 4561355 |
| correction of fallot's tetralogy after palliative operations. | during the period 1960 to 1978, 98 patients underwent intracardiac repair of fallot's tetralogy after palliative operations. preoperative symptoms were cyanosis, dyspnea, increased fatigue with squatting and hypoxic spells. the hemoglobin concentration varied from 19 to 22 g/100 ml. at correction only 65 of 95 shunts were patent and needed surgical closure. seventeen early deaths occurred (19%), the main causes being cardiac failure and arrhythmia. one patient died 3 years after correction from ... | 2000 | 87399 |
| active chronic hepatitis and pyoderma gangrenosum: report of a case. | | 2004 | 535175 |
| repeated hepatic ischemia as a treatment for carcinoid liver metastases. | hepatic ischemia has been used in the treatment of bilobar malignant carcinoid tumors in the liver. hepatic artery ligation, hepatic dearterialization, and embolization with nondegradable micromaterial have been followed by collateral formation and are associated with a high complication rate. to reduce this, an implantable vascular occluder permitting intermittent occlusion of the hepatic artery was used in 3 patients with bilateral malignant carcinoid in the liver, with high urinary excretion ... | 2009 | 2741468 |
| a long term follow up of resection interposition arthroplasty of the knee using chromicized fascia lata. | we have reviewed the results of 25 patients who had a resection interposition arthroplasty of the knee using chromicized fascia lata, with a mean follow-up of 22 years. no patient had severe pain although 10 had occasional pain after heavy labour. thirteen knees had 60 degrees of movement or more but 12 had 45 degrees or less. the radiographs all showed adaptive remodelling of the resected bone ends. this procedure successfully restored function in the majority of cases with absence of pain over ... | 2012 | 3372105 |
| stress and medical illness in the elderly. | | 2013 | 3553904 |
| interactions of oncogenes with haematopoietic cells. | | 2013 | 3536235 |
| [prognostic significance of qualitative and quantitative changes in basophils in chronic myeloleukoses and other neoplasms]. | | 2014 | 4184587 |
| the histaminase activity of ceruloplasmin. | | 2015 | 4630290 |
| vd--report it. | | 2016 | 5067414 |
| a new microsurgical needleholder. | microsurgery demands precise and rapid manipulation of needle and thread with maintenance of special respect for the fragility of the tissues. the design of the needleholder is of paramount importance in achieving these goals. the instrument described below is light, long enough to rest comfortably in the hand, and balanced in such a way that it can be manipulated into awkward corners with relative ease. | 1991 | 269711 |
| antibiotic usage surveillance: an overview of the issues. | | 1998 | 121379 |
| use of the fluorochrome 4'6-diamidino-2-phenylindole in genetic and developmental studies of chloroplast dna. | use of the dna-specific fluorochrome 4'6-diamidino-2-phenylindole (dapi) makes it possible to examine in situ the structure of chloroplast dna (chdna) with the fluorescence microscope. this simplifies the study of genetic and developmental changes in chloroplast dna. three examples are presented. (a) wild-type euglena gracilis b contains several chloroplast dna nucleoids per chloroplast. a yellow mutant lacking functional chloroplasts is similar, but such nucleoids are absent in an aplastidic mu ... | 2000 | 90051 |
| [a histological study on urethral stricture (author's transl]. | | 2000 | 86632 |
| the surgical management of low back and sciatic syndrome in disc disease or injury : results of a joint neurosurgical and orthopaedic project. | | 2002 | 127575 |
| nonunion of intercondylar eminence fracture of the tibia. | an unusual case of symptomatic nonunion of an intercondylar eminence fracture in an adult is presented. the nonunion was successfully treated using internal fixation and bone grafting. | 2009 | 3290508 |
| [periarthritis of the shoulder. symptomology and classification]. | | 2011 | 3187101 |
| [occupational asthma in the food industry]. | | 2011 | 3067294 |
| artificial insemination with cryopreserved donor semen: a decade of experience. | ten years' experience of artificial insemination with cryopreserved donor semen for 1023 courses in 783 women resulting in 572 pregnancies is reported. a simple approach with multiple inseminations timed on the basis of cycle length, temperature charts, mucus symptoms and signs was used. the life table pregnancy rate was 61% after 12 cycles of treatment and 75% after 24 cycles. women had a significantly higher rate of pregnancy in second and subsequent courses of treatment, and the pregnancy rat ... | 2012 | 3382608 |
| the hemodynamic consequences of high-dose thiopental anesthesia. | the hemodynamic and electroencephalographic effects of a 60 min infusion of thiopental, given at the rate of 1.25 mg x kg-1 x min-1 (total dose 75 mg/kg), were studied in 10 patients without cardiorespiratory disease undergoing surgery for the removal of large and/or deeply seated arteriovenous malformations. data on heart rate, arterial, right atrial, pulmonary arterial, and pulmonary capillary wedge pressures, thermodilution cardiac output (expressed as cardiac index), the electroencephalogram ... | 2014 | 4014729 |
| interactions between nutrition and heredity in coronary heart disease. | | 2015 | 4588183 |