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isolation of erwinia chrysanthemi kdud mutants altered in pectin degradation.mutants of erwinia chrysanthemi impaired in pectin degradation were isolated by chemical and mu d(ap lac) insertion mutagenesis. a mutation in the kdud gene coding for 2-keto-3-deoxygluconate oxidoreductase prevented the growth of the bacteria on polygalacturonate as the sole carbon source. analysis of the kdud::mu d(ap lac) insertions indicated that kdud is either an isolated gene or the last gene of a polycistronic operon. some of the mu d(ap lac) insertions were kdud-lac fusions in which beta ...19863949717
erwinia herbicola as a cause of bacterial endocarditis.a case of endocarditis due to erwinia herbicola is reported. three years previously the patient had been fitted with a porcine xenograft.19863958506
hypersensitivity pneumonitis in grain farmers due to sensitization to erwinia herbicola.two cases of hypersensitivity pneumonitis in grain workers are described. both cases had evidence of sensitization to the gram-negative bacteria erwinia herbicola as judged by skin tests, detection of serum precipitins, and inhalation challenge. e. herbicola is frequently found in the microflora of grains.19853966692
isolation and characterization of erwinia chrysanthemi mutants defective in degradation of hexuronates.spontaneous and tn9-induced mutants of erwinia chrysanthemi were isolated which affect the degradative pathway of galacturonate and ketodeoxygluconate. the mutations were characterized both biochemically and functionally by complementation analysis and localized in the e. chrysanthemi chromosome. the kdgk gene mapped very close to ile, the kdga gene was between trp and his, and the exut-uxac-uxab-uxaa cluster was linked to thy. the different types of mutants obtained were consistent with an orga ...19853968035
efficient transformation of erwinia carotovora subsp. carotovora and e. carotovora subsp. atroseptica.we used a modified version of the method of hanahan (d. hanahan, j. mol. biol. 166:557-580, 1983) to transform erwinia carotovora subsp. carotovora and e. carotovora subsp. atroseptica with the plasmids pbr322, pbr325, and pat153. the transformation frequency ranged from 1 x 10(2) to 4 x 10(4) colonies per micrograms of plasmid dna. the nature of these transformants was confirmed by plasmid analysis. cole1-based plasmids make potentially useful cloning vectors for the study of genes involved in ...19853968041
structure of the core oligosaccharide from lipopolysaccharide of erwinia carotovora.the lipopolysaccharide of erwinia carotovora was analyzed by quantitative sugar analysis, methylation analysis, and chromic oxide oxidation. this led to the following structure of the core oligosaccharide: (formula; see text).19853972773
erwinia herbicola isolates from alfalfa plants may play a role in nodulation of alfalfa by rhizobium meliloti.erwinia herbicola was isolated from roots of plants derived from surface-sterilized seeds of all alfalfa varieties that were tested. some of these e. herbicola strains affected nodulation by certain strains of rhizobium meliloti. in previously published work we presented the isolation of slow-and fast-nodulating variants from a single culture of r. meliloti 102f51. in the absence of e. herbicola, the slow-nodulating variant induced the formation of nodules on alfalfa as rapidly as the faster-nod ...19854004214
bacteriocin-resistant mutants of erwinia chrysanthemi: possible involvement of iron acquisition in phytopathogenicity.a series of bacteriocin-resistant mutants of erwinia chrysanthemi 3937jrh were unable to elicit soft-rot symptoms on saintpaulia plants. the loss of pathogenicity was correlated with the disappearance of one to three outer membrane polypeptides (molecular weights, about 80,000 to 90,000) whose production in wild-type strains was greatly enhanced under iron-limited growth conditions. the mutants did not exhibit altered extracellular pectinolytic or cellulolytic activities.19854008442
reca is required in the induction of pectin lyase and carotovoricin in erwinia carotovora subsp. carotovora.pectin lyase (pnl) and the bacteriocin carotovoricin (ctv) were induced in erwinia carotovora subsp. carotovora 71 by the dna-damaging agents mitomycin c, nalidixic acid, and uv light. to determine whether the reca product was involved in the expression of these damage-inducible phenotypes, we cloned the e. carotovora subsp. carotovora reca+ gene, inactivated it by tn5 insertion, and constructed an e. carotovora subsp. carotovora reca::tn5 strain by gene replacement via homologous recombination. ...19854044526
heterologous hybridization of bacterial dna to the endoglucanases a and b structural genes cela and celb of clostridium thermocellum.dna from various cellulolytic and non-cellulolytic bacteria was found to hybridize to clostridium thermocellum ncib10682 dna fragments carrying the structural genes cela and celb which code for endoglucanases a and b. homology to cela was detected in agrobacterium rhizogenes, azospirillum brasilense, bacillus subtilis, cellulomonas sp., clostridium stercorarium, erwinia chrysanthemi, pseudomonas solanacearum and streptomyces griseus. homology to celb was detected only in b. subtilis, c. stercora ...19854083831
separation of escherichia adecarboxylata from the "erwinia herbicola-enterobacter agglomerans" complex and from the other enterobacteriaceae by nucleic acid and protein electrophoretic techniques.the species escherichia adecarboxylata was examined for dna relatedness to the "erwinia herbicola-enterobacter agglomerans" complex and to other members of the family enterobacteriaceae. dna-dna hybridizations (nitrocellulose filter method) showed that strains received as e. adecarboxylata were highly related to each other (73-100% homology). three strains of e. agglomerans and one strain of e. herbicola showed, respectively, 77, 96, 97 and 92% relatedness with the labelled dna of e. adecarboxyl ...19854083833
ultrastructure of the cell wall of erwinia amylovora.thin sectioned cells of erwinia amylovora revealed two electron-dense layers in their walls when fixed at 24 to 27 c and three when fixed at 4 c.19714105035
enzyme evolution in the enterobacteriaceae.an immunological approach has been used for the study of alkaline phosphatase evolution in bacteria of the family enterobacteriaceae. antisera were prepared against alkaline phosphatase from escherichia coli and klebsiella aerogenes and tested against the unpurified alkaline phosphatases of 32 strains of enterobacteria by double diffusion and quantitative micro-complement fixation. the immunological relationships detected among the alkaline phosphatases of enterobacteria agree approximately with ...19724113124
ultrastructure of the cell wall and cytoplasmic membrane of gram-negative bacteria with different fixation techniques.the ultrastructure of the cytoplasmic membrane and cell wall of two strains of escherichia coli, proteus morganii, p. vulgaris, acinetobacter anitratum, moraxella lacunata, erwinia amylovora, acinetobacter sp., and of a plant pathogen, unclassified gram-negative, fixed by the ryter-kellenberger procedure, was found to be significantly affected by the use or omission of the uranyl postfixation included in that procedure, and by the presence or absence of calcium in the oso(4) fixative. the omissi ...19734120570
bacteria from intravenous fluids. 19734124772
characteristics of erwinia amylovora bacteriophage and its possible role in the epidemology of fire blight. 19734125539
immunological tolerance to erwinia carotovora l-asparaginase. 19734128407
letter: flower vases and pathogens. 19744132276
[comparison of some methods of staining the flagella of phytopathogenic bacteria of the genera erwinia and pseudomonas]. 19724134018
the passive cutaneous anaphylaxis test (pca) in the diagnosis of allergic respiratory diseases caused by hypersensitivity to organic dust. 19744135106
etiology of some bacterial diseases of poplar in poland. i. bacteriosis caused by pseudomonas migula and erwinia winslow. 19674166073
a new l-asparaginase with antitumour activity? 19684175572
association of virulence characteristics of erwinia amylovora with toxigenicity of its phage lysates to rabbit. 19734199439
enzymes as immunosuppressants: basic considerations. 19734199577
further investigations into the teratogenic potential of imperfect potatoes. 19734200996
synthesis of antimicrobial nitroimidazolyl 2-sulfides, -sulfoxides, and -sulfones. 19734201423
chemotactic responses by motile bacteria. 19734202782
multiplication of nosocomial pathogens in intravenous feeding solutions.a major problem in total parenteral nutrition is sepsis, particularly that caused by candida. studies of four solutions, a casein hydrolysate, a fibrin hydrolysate, and two crystalline amino acid solutions, show that the protein hydrolysate solutions appear to be highly selective for candida over bacteria, whereas the crystalline amino acid solutions are not. these findings suggest that the crystalline amino acid preparations may offer a partial solution to the infection problem by minimizing th ...19734203333
synthesis of 2-oxygenated glycyrrhetic acid derivatives. 19744203365
investigations into the teratogenic potential of imperfect potatoes. 19734203408
the presence and possible significance of cross-reactive antigens in rhizobium--legume associations. 19734203452
effect of iron deprivation and temperature upon bacterial survival. 19734204389
bacteremia caused by gram-negative bacilli. 19744207136
variation in the occurrence of extracellular diffusible antigens in temperature-induced variants of erwinia herbicola y46, and observations of their relationships with erwinia amylovora. 19744208906
release of enzymes from cell walls by an endopectate-trans-eliminase. 19744215025
growth abnormalities in microorganisms exposed to tobacco leaf extracts. 19744215552
erwinia infections in man. 19734217239
cell division in a species of erwinia. 13. leakage of proteins from the peripheral area of filamentous cells. 19724259818
intrageneric clustering and divergence of erwinia strains from plants and man in the light of deoxyribonucleic acid segmental homology. 19744279767
[nonphytopathogenous erwinias of the group "lathyri-herbicola" isolated from clinical sources. their morphological and physiological characteristics and sensitivity to antibiotics (author's transl)]. 19744280947
inhibition of rd/3 rat sarcoma by l-asparaginase alone and in combination with sodium para-amino-salicylate. 19734281176
phosphatases: applications of new methods for their separation. 19714326442
glucose fermentation endproducts of erwinia spp. and other enterobacteria. 19714329516
[study of penicillin-acylase produced by erwinia aroideae]. 19714335226
clinical and bacteriologic observations on the proposed species, enterobacter agglomerans (the herbicola-lathyri bacteria). 19724344106
heterologous protein-rna interactions in bacterial ribosomes. 19734356720
microbial metabolism of amino alcohols. metabolism of ethanolamine and 1-aminopropan-2-ol in species of erwinia and the roles of amino alcohol kinase and amino alcohol o-phosphate phospho-lyase in aldehyde formation.1. growth of erwinia carotovora n.c.p.p.b. 1280 on media containing 1-aminopropan-2-ol compounds or ethanolamine as the sole n source resulted in the excretion of propionaldehyde or acetaldehyde respectively. the inclusion of (nh(4))(2)so(4) in media prevented aldehyde formation. 2. growth, microrespirometric and enzymic evidence implicated amino alcohol o-phosphates as aldehyde precursors. an inducibly formed atp-amino alcohol phosphotransferase was partially purified and found to be markedly s ...19734357716
pyruvic acid metabolism and ethanol formation in erwinia amylovora. 19714400187
the effect of temperature on the growth of the fireblight pathogen, erwinia amylovora. 19744436163
soft rot of hyacinths in the ukraine]. 19744444594
isolation and identification of erwinia in a hospital laboratory. 19744444676
[ultrastructure and chemical composition of lipopolysaccharide from erwinia carotovora]. 19744453202
[ribosome-like particles in the mesosomes of erwinia nimipressuralis 362]. 19744465656
[comparative characteristics of the structure of erwinia nimipressuralis and the localization of esterases in bacteria with varying virulence]. 19744465674
[bacterial dropsy of popular and its causative agent]. 19744465676
[isolation of the phages of phytopathogenic bacteria]. 19744465706
effect of carbon and nitrogen sources on the production of l-asparaginase by erwinia aroideae. 19724516164
opportunistic infections with erwinia-like species. 19734517131
physical properties and subunit structure of l-asparaginase isolated from erwinia carotovora.1. l-asparaginases from erwinia carotovora and escherichia coli (ec2 enzyme) are both capable of inhibiting and eliminating certain types of tumour cells. the er. carotovora enzyme is a more basic protein, however, and in contrast with the ec2 enzyme it contains neither tryptophan nor cystine, and disulphide bonds are therefore absent. the molecule is very stable in solution from ph3.0 to about ph12.0, and is somewhat more stable at alkaline ph than is the esch. coli enzyme. calculations based o ...19724551660
a pectate gel medium for the isolation and diagnosis of pectinolytic bacteria. 19724558959
erwinia carotovora asparaginase in patients with prior anaphylaxis to asparaginase from e. coli. 19724559405
physical properties and subunit structure of l-asparaginase isolated from erwinia carotovora. 19724561025
in vitro blastogenesis inhibited by erwinia carotovora l-asparaginase. 19724561972
transfer among erwinia spp. and other enterobacteria of antibiotic resistance carried on r factors.antibiotic resistance carried on r factors was transferred by conjugation from escherichia coli b/r and shigella flexneri 1a to erwinia spp. tetracycline resistance (tetr) carried on r factor r100 drd-56 was transferred from e. coli b/r to strains of erwinia amylovora, e. aroideae, e. atroseptica, e. chrysanthemi, e. cytolytica, e. dissolvens, e. herbicola, e. nigrifluens, and e. nimipressuralis, but not to strains of erwinia carotovora, e. carnegieana, e. dieffenbachiae, e. oleraceae, and e. qu ...19724562410
the genus erwinia: enterobacteria pathogenic to plants and animals. 19724562815
comparison of different techniques for isoelectric focusing on polyacrylamide gel slabs using bacterial asparaginases. 19724563222
hospital airborne bacteria as estimated by the andersen sampler versus the gravity settling culture plate. 19724565890
fermentation kinetics and continuous process of l-asparaginase production.for the purpose of obtaining l-asparaginase in quantities from erwinia aroideae, cell growth and enzyme formation were investigated in both batch and continuous fermentation. using yeast extract as a growth-limiting substrate, the relationship between specific growth rate and substrate concentration was found to fit the monod equation. the optimum temperature for enzyme production was 24 c, although cell growth was higher at 28 c. the enzyme yield reached its maximum of 4 iu/ml during the negati ...19734568894
the effects of immunization to l-asparaginase on antitumor and enzymatic activity. 19734569788
effects of calcium and magnesium ions and host viability on growth of bdellovibrios. 19734570839
l-asparaginase from erwinia carotovora. physicochemical properties of the native and succinylated enzyme. 19734573979
physiology of the bdellovibrios. 19724579073
transmission of lac by the sex factor e in erwinia strains from human clinical sources.lactose-utilizing (lac(+)) strains of erwinia spp. from human clinical material transfer lac by conjugation to plant strains of erwinia herbicola and erwinia amylovora, to other erwinia strains from human clinical sources, and also to escherichia coli, paracolobactrum arizonae, salmonella typhimurium, and shigella dysenteriae. the frequency of this transfer varies with the donor and recipient strains employed. the lac genes appear stable in these exconjugants, and they are not cured by acridine ...19734582635
[transmission of extrachromosomal resistance to antibiotics from escherichia coli to erwinia carotovora f. citrullis by the method of conjugation]. 19734585591
the apparent conservation of the internal low efficiency promoter of the tryptophan operons of several species of enterobacteriaceae.the specific activities of the tryptophan biosynthetic enzymes were determined for eleven different species of enterobacteriaceae grown under repressing and non-repressing conditions. in all the bacteria examined the multipliciy of derepression for the first two enzymes of the pathway was at least twofold greater than the multiplicity of derepression for the last three enzymes.19734587116
the ortho-nitrophenol (onpg) test and acid from lactose in gram-negative genera.the results are given of the ortho-nitrophenol test for beta-galactosidase production (onpg test) on 588 strains of 123 aerobic species of bacteria, representing 30 genera. apart from some strains of erwinia herbicola (synonym chromobacterium typhiflavum) and of yersinia spp, these strains were not members of the enterobacteriaceae, in which family the onpg test is widely used and well documented. the strains were also tested for acid production from 1, 5, and 10% lactose and the findings are di ...19734587939
estimating the density of individual bacterial populations introduced into natural ecosytems. 19734588655
immunological and electrophoretical comparison of ribosomal proteins from eight species belonging to enterobacteriaceae. 19734589346
genetic transfer of episomic elements among erwinia species and other enterobacteria: f'lac+.the episomic element f'lac(+) was transferred, probably by conjugation, from escherichia coli to lac(-) strains of erwinia herbicola, erwinia amylovora, and erwinia chrysanthemi (but not to several other erwinia spp. in preliminary trials). the lac genes in the exconjugants of the erwinia spp. showed varying degrees of stability depending on the strain (stable in e. herbicola strains y46 and y74 and e. amylovora strain ea178, but markedly unstable in e. chrysanthemi strain ec16). the lac genes a ...19724591473
therapy with erwinia l-asparaginase in children with acute leukemia after anaphylaxis to e. coli l-asparaginase. 19744592901
properties of erwinia atroseptica and erwinia carotovora. 19734595821
comparison of the l-asparaginases from escherichia coli and erwinia carotovora as immunosuppressants. 19744596965
direct selection for p1-sensitive mutants of enteric bacteria.a method has been developed to isolate mutants sensitive to coliphage p1 from bacterial genera normally not sensitive to this phage. p1clr100km was used. this phage is heat inducible and confers kanamycin resistance when present as a prophage (in lysogens). p1-sensitive mutants of klebsiella, enterobacter, citrobacter, and erwinia have been found. this technique provides a well-known genetic system for the study of many bacterial genera that previously had either no such system or only a margina ...19744598005
clinicopathologic and ultrastructural studies of l-asparaginase-induced hypocalcemia in rabbits. an experimental animal model of acute hypoparathyroidism. 19734598146
a simple and rapid method for the estimation of l-asparaginase in chromatographic and electrophoretic effluents: comparison with other methods. 19744599360
[extracellular accumulation of valine by bacteria of the family enterobacteriaceae that form acetone]. 19744603535
erwinia rhapontici (millard) burkholder associated with pink grain of wheat. 19744608652
effect of carbobenzoxy, phenylacetyl and phenylpropionyl derivatives of amino acids on mammalian and bacterial asparaginase. 19744611786
in-use contamination of intravenous infusion fluid.during the 1970 to 1971 nationwide epidemic of septicemias caused by enterobacter cloacae and enterobacter agglomerans traced to intrinsic contamination of abbott intravenous infusion products, 94 infusion systems manufactured by baxter laboratories were studied microbiologically and epidemiologically during hospital use. intravenous fluid from 10 systems (11%) contained microorganisms, usually staphylococcus or bacillus species; one infusion was heavily contaminated with klebsiella pneumoniae. ...19744613269
[study of the flocculation effectiveness of water-soluble polyelectrolytes in the processing of native solutions of antiotics]. 19744616642
[a new species of enterobacteria found in medical bacteriology: enterobacter agglomerans (ex-erwinia herbicola). biochemical study of 205 strains]. 19744617535
effect of selected herbicides on bacterial growth rates.specific growth rate constants were used to evaluate the effects of selected herbicides on erwinia carotovora, pseudomonas fluorescens, and bacillus sp. comparison of growth rate constants permitted the identification of either stimulatory or inhibitory effects of these substances. e. carotovora was inhibited by 6,7-dihydrodipyrido(1,2-a:2'-c)pyrazinediium (diquat) and 4-hydroxy-3,5-diiodobenzonitrile (ioxynil) at 25 mug/ml; 1,1'-dimethyl-4,4'-bipyridinium (paraquat) at 50 mug/ml; and pentachlor ...19724622833
a colorimetric technique for the demonstration of l-asparaginase in electropherograms. 19724626844
contaminated stethoscopes: a potential source of nosocomial infections. 19724632289
sepsis caused by contaminated intravenous fluids. epidemiologic, clinical, and laboratory investigation of an outbreak in one hospital. 19724644166
purification and properties of l-asparaginase of erwinia aroideae. 19724649741
[antibacterial action of anilide group substances on phytopathogenic bacteria]. 19724663488
antibiotics and bleaching powder in the control of bacterial stalk rot of maize. 19724665134
electron microscopy of lipopolysaccharides from erwinia carotovora and the bacillary and l forms of agrobacterium tumefaciens. 19724673374
[synthesis of various extracellular enzymes by erwinia carotovora]. 19734717392
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