| a theoretical study on the metabolic requirements resulting from alpha-ketoglutarate-dependent cleavage of phenoxyalkanoates. | the etherolytic cleavage of phenoxyalkanoic acids in various bacteria is catalyzed by an alpha-ketoglutarate-dependent dioxygenase. in this reaction, the electron acceptor is oxidatively decarboxylated to succinate, whereas the proper substrate is cleaved by forming the oxidized alkanoic acid and the phenolic intermediate. the necessity of regenerating alpha-ketoglutarate and the consequences for the overall metabolism were investigated in a theoretical study. it was found that the dioxygenase m ... | 2000 | 10618245 |
| controlled clinical comparison of plastic and glass bottles of bact/alert fa medium for culturing organisms from blood of adult patients. | a new, clear-plastic nonvented aerobic fa bottle, designed to prevent breakage, has been developed for the bact/alert blood culture system. we assessed the new plastic fa bottle by comparing its performance with that of the current glass fa bottle for recovery of microorganisms and time to detection of growth in blood samples obtained for culture from adult patients with suspected bloodstream infections. we conclude that the bact/alert plastic and glass fa bottles are comparable for recovery of ... | 2005 | 15815032 |
| dna pyrosequencing-based bacterial pathogen identification in a pediatric hospital setting. | sole reliance on biochemical methods can limit the clinical microbiology laboratory's ability to identify bacterial pathogens. this study describes the incorporation of dna pyrosequencing-based identification for routine pathogen identification of atypical clinical isolates in a large children's hospital. the assay capitalized on the highly conserved nature of 16s rrna genes by positioning amplification and sequencing primers in conserved target sequences flanking the variable v1 and v3 regions. ... | 2007 | 17652476 |
| validation of vitek 2 nonfermenting gram-negative cards and vitek 2 version 4.02 software for identification and antimicrobial susceptibility testing of nonfermenting gram-negative rods from patients with cystic fibrosis. | accurate identification and antimicrobial susceptibility testing (ast) of nonfermenters from cystic fibrosis patients are essential for appropriate antimicrobial treatment. this study examined the ability of the newly designed vitek 2 nonfermenting gram-negative card (ngnc) (new gram-negative identification card; biomérieux, marcy-l'etoile, france) to identify nonfermenting gram-negative rods from cystic fibrosis patients in comparison to reference methods and the accuracy of the new vitek 2 ver ... | 2009 | 19710272 |
| beta-lactam antibiotics: from antibiosis to resistance and bacteriology. | this review focuses on the era of antibiosis that led to a better understanding of bacterial morphology, in particular the cell wall component peptidoglycan. this is an effort to take readers on a tour de force from the concept of antibiosis, to the serendipity of antibiotics, evolution of beta-lactam development, and the molecular biology of antibiotic resistance. these areas of research have culminated in a deeper understanding of microbiology, particularly in the area of bacterial cell wall s ... | 2010 | 20041868 |
| multidrug-resistance and toxic metal tolerance of medically important bacteria isolated from an aquaculture system. | the use of antimicrobials and toxic metals should be considered carefully in aquaculture and surrounding environments. we aimed to evaluate medically relevant bacteria in an aquaculture system and their susceptibility to antimicrobials and toxic metals. selective cultures for enterobacteria (ent), non-fermenting gram-negative rods (nfr) and gram-positive cocci (gpc) were obtained from water samples collected in two different year seasons. the isolated bacteria were biochemically identified and a ... | 2012 | 22972388 |
| pandoraea pulmonicola chronic colonization in a cystic fibrosis patient, france. | pandoraea are considered emerging multidrug resistant pathogens in the context of cystic fibrosis. we report herein for the first time the case of a 30-year-old woman with cystic fibrosis, living in france, who was chronically infected with pandoraea pulmonicola and who died of pseudomonas aeruginosa sepsis 3 weeks after bilateral lung transplantation. | 2013 | 25356323 |
| structural studies of pseudomonas and chromobacterium ω-aminotransferases provide insights into their differing substrate specificity. | the crystal structures and inhibitor complexes of two industrially important ω-aminotransferase enzymes from pseudomonas aeruginosa and chromobacterium violaceum have been determined in order to understand the differences in their substrate specificity. the two enzymes share 30% sequence identity and use the same amino acceptor, pyruvate; however, the pseudomonas enzyme shows activity towards the amino donor β-alanine, whilst the chromobacterium enzyme does not. both enzymes show activity toward ... | 2013 | 23519665 |
| evaluation of a simple blood culture amplification and antigen detection method for diagnosis of salmonella enterica serovar typhi bacteremia. | in most areas where typhoid is endemic, laboratory diagnosis is not possible due to the lack of appropriate facilities. we investigated whether the combination of blood culture amplification of salmonella enterica serovar typhi with an s. typhi antigen rapid diagnostic test (rdt) could be an accurate and inexpensive tool for the accelerated diagnosis of patients with acute typhoid in laos. for a panel of 23 gram-negative reference pathogens, the standard diagnostics (catalog no. 15fk20; kyonggi- ... | 2013 | 23100346 |
| biochemical characteristics, adhesion, and cytotoxicity of environmental and clinical isolates of herbaspirillum spp. | herbaspirillum bacteria are best known as plant growth-promoting rhizobacteria but have also been recovered from clinical samples. here, biochemical tests, matrix-assisted laser deionization-time of flight (maldi-tof) mass spectrometry, adherence, and cytotoxicity to eukaryotic cells were used to compare clinical and environmental isolates of herbaspirillum spp. discrete biochemical differences were observed between human and environmental strains. all strains adhered to hela cells at low densit ... | 2014 | 25355763 |
| biochemical characteristics, adhesion, and cytotoxicity of environmental and clinical isolates of herbaspirillum spp. | herbaspirillum bacteria are best known as plant growth-promoting rhizobacteria but have also been recovered from clinical samples. here, biochemical tests, matrix-assisted laser deionization-time of flight (maldi-tof) mass spectrometry, adherence, and cytotoxicity to eukaryotic cells were used to compare clinical and environmental isolates of herbaspirillum spp. discrete biochemical differences were observed between human and environmental strains. all strains adhered to hela cells at low densit ... | 2014 | 25355763 |
| isolation, identification and characterization of burkholderia pseudomallei from soil of coastal region of india. | melioidosis is an emerging infectious disease caused by a free living soil dwelling gram-negative bacterium burkholderia pseudomallei. the disease is endemic to most parts of southeast asia and northern australia and the organism has been isolated from moist soil and water. in india clinical cases are recently reported from the states of tamilnadu, kerala, karnataka, maharashtra, orissa, assam, west bengal, pondicherry and tripura. this study is aimed to confirm the prevalence of this important ... | 2014 | 25187882 |
| the effect of spent bleaching earth ageing process on its physicochemical and microbial composition and its potential use as a source of fatty acids and triterpenes. | this study was aimed at investigating the physicochemical and microbiological changes that took place during the ageing process of spent bleaching earth in the presence of autochthonous microorganisms. research material included fresh spent bleaching earth (sbe0) and the same material after 3 years of storage at the constant temperature of 20 °c, without aeration and moistening (sbe3). changes in the chemical composition of analysed waste material were observed during its ageing process point to ... | 2014 | 24875308 |
| novel real-time pcr detection assay for brucella suis. | brucella suis is the causative agent of brucellosis in suidae and is differentiated into five biovars (bv). biovars 1 and 3 possess zoonotic potential and can infect humans, whereas biovar 2 represents the main source of brucellosis in feral and domestic pigs in europe. both aspects, the zoonotic threat and the economic loss, emphasize the necessity to monitor feral and domestic pig populations. available serological or pcr based methods lack sensitivity and specificity. | 2015 | 26392898 |
| the microbiome of the upper airways: focus on chronic rhinosinusitis. | upper airway diseases including allergic rhinitis, chronic rhinosinusitis with or without polyps, and cystic fibrosis are characterized by substantially different inflammatory profiles. traditionally, studies on the association of specific bacterial patterns with inflammatory profiles of diseases had been dependent on bacterial culturing. in the past 30 years, molecular biology methods have allowed bacterial culture free studies of microbial communities, revealing microbiota much more diverse th ... | 2015 | 25624972 |
| outbreak of achromobacter xylosoxidans and ochrobactrum anthropi infections after prostate biopsies, france, 2014. | we report an outbreak of healthcare-associated prostatitis involving rare environmental pathogens in immunocompetent patients undergoing transrectal prostate biopsies at hôpital édouard herriot (lyon, france) during august 13-october 10, 2014. despite a fluoroquinolone-based prophylaxis, 5 patients were infected with achromobacter xylosoxidans and 3 with ochrobactrum anthropi, which has not been reported as pathogenic in nonimmunocompromised persons. all patients recovered fully. analysis of the ... | 2016 | 27434277 |
| bloodstream infections after solid-organ transplantation. | solid-organ transplantation (sot) has become the preferred strategy to treat a number of end-stage organ disease, because a continuous improvement in survival and quality of life. while preventive strategies has decreased the risk for classical opportunistic infections (such as viral, fungal and parasite infections), bacterial infections, and particularly bloodstream infections (bsis) remain the most common and life-threatening complications in sot recipients. the source of bsi after transplant ... | 2016 | 26766415 |
| origin and evolution of antibiotic resistance: the common mechanisms of emergence and spread in water bodies. | the environment, and especially freshwater, constitutes a reactor where the evolution and the rise of new resistances occur. in water bodies such as waste water effluents, lakes, and rivers or streams, bacteria from different sources, e.g., urban, industrial, and agricultural waste, probably selected by intensive antibiotic usage, are collected and mixed with environmental species. this may cause two effects on the development of antibiotic resistances: first, the contamination of water by antib ... | 2012 | 22303296 |
| quorum sensing and self-quorum quenching in the intracellular pathogen brucellamelitensis. | brucella quorum sensing has been described as an important regulatory system controlling crucial virulence determinants such as the virb type iv secretion system and the flagellar genes. however, the basis of quorum sensing, namely the production of autoinducers in brucella has been questioned. here, we report data obtained from the use of a genetic tool allowing the in situ detection of long-chain n-acyl-homoserine lactones (ahl) activity at single bacterium level in brucella melitensis. these ... | 2013 | 24349302 |
| vim-47, a new variant of the autochthonous metallo-β-lactamase vim-13 from the balearic islands in spain. | | 2016 | 26976859 |
| detection and diversity of expressed denitrification genes in estuarine sediments after reverse transcription-pcr amplification from mrna. | the expression of five denitrification genes coding for two nitrate reductases (narg and napa), two nitrite reductases (nirs and nirk), and nitrous oxide reductase (nosz) was analyzed by reverse transcription (rt)-pcr of mrna extracted from two sediment samples obtained in the river colne estuary (united kingdom), which receives high nitrogen inputs and for which high denitrification rates have been observed. the presence of all five genes in both sediment samples was confirmed by pcr amplificat ... | 2002 | 12324352 |
| clinical investigation of an outbreak of alveolitis and asthma in a car engine manufacturing plant. | exposure to metal working fluid (mwf) has been associated with outbreaks of extrinsic allergic alveolitis (eaa) in the usa, with bacterial contamination of mwf being a possible cause, but is uncommon in the uk. twelve workers developed eaa in a car engine manufacturing plant in the uk, presenting clinically between december 2003 and may 2004. this paper reports the subsequent epidemiological investigation of the whole workforce. the study had three aims: (1) to measure the extent of the outbreak ... | 2007 | 17504818 |
| characterization, crystallization and preliminary x-ray diffraction analysis of an (s)-specific esterase (pfesta) from pseudomonas fluorescens kctc 1767: enantioselectivity for potential industrial applications. | the structures and reaction mechanisms of enantioselective hydrolases, which can be used in industrial applications such as biotransformations, are largely unknown. here, the x-ray crystallographic study of a novel (s)-specific esterase (pfesta) from pseudomonas fluorescens kctc 1767, which can be used in the production of (s)-ketoprofen, is described. multiple sequence alignments with other hydrolases revealed that pfesta contains a conserved ser67 within the s-x-x-k motif as well as a highly c ... | 2012 | 23143253 |
| a novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in escherichia coli and tobacco plants. | a key enzyme in the shikimate pathway, 5-enolpyruvylshikimate-3-phosphate synthase (epsps) is the primary target of the broad-spectrum herbicide glyphosate. identification of new aroa genes coding for epsps with a high level of glyphosate tolerance is essential for the development of glyphosate-tolerant crops. in the present study, the glyphosate tolerance of five bacterial aroa genes was evaluated in the e. coli aroa-defective strain er2799 and in transgenic tobacco plants. all five aroa genes ... | 2012 | 22715408 |
| ambulatory pediatric oncology clabsis: epidemiology and risk factors. | to compare the burden of central line-associated bloodstream infections (clabsis) in ambulatory versus inpatient pediatric oncology patients, and identify the epidemiology of and risk factors associated with ambulatory clabsis. | 2013 | 23881643 |
| inhaled, dual release liposomal ciprofloxacin in non-cystic fibrosis bronchiectasis (orbit-2): a randomised, double-blind, placebo-controlled trial. | the delivery of antipseudomonal antibiotics by inhalation to pseudomonas aeruginosa-infected subjects with non-cystic fibrosis (cf) bronchiectasis is a logical extension of treatment strategies successfully developed in cf bronchiectasis. dual release ciprofloxacin for inhalation (drcfi) contains liposomal ciprofloxacin, formulated to optimise airway antibiotic delivery. | 2013 | 23681906 |
| fluorescent multiple staining and casa system to assess boar sperm viability and membranes integrity in short and long-term extenders. | the aim of this study was to assess the effect on boar spermatozoa quality of in vitro storage in short and long-term extenders by fluorescent multiple staining (fms) and computer assisted semen analyzer (casa). fresh ejaculates from three healthy, sexually mature boars were diluted with equal volumes of six short-term or three long-term commercial extenders and stored at 19°c for 6 days (short-term) or 12 days (long-term). the integrity of spermatozoa membranes was analyzed by fms using propidi ... | 2013 | 26623308 |
| lung phenotype of juvenile and adult cystic fibrosis transmembrane conductance regulator-knockout ferrets. | chronic bacterial lung infections in cystic fibrosis (cf) are caused by defects in the cf transmembrane conductance regulator chloride channel. previously, we described that newborn cf transmembrane conductance regulator-knockout ferrets rapidly develop lung infections within the first week of life. here, we report a more slowly progressing lung bacterial colonization phenotype observed in juvenile to adult cf ferrets reared on a layered antibiotic regimen. even on antibiotics, cf ferrets were s ... | 2014 | 24074402 |
| bacteria associated with amblyomma cajennense tick eggs. | ticks represent a large group of pathogen vectors that blood feed on a diversity of hosts. in the americas, the ixodidae ticks amblyomma cajennense are responsible for severe impact on livestock and public health. in the present work, we present the isolation and molecular identification of a group of culturable bacteria associated with a. cajennense eggs from females sampled in distinct geographical sites in southeastern brazil. additional comparative analysis of the culturable bacteria from an ... | 2015 | 26537602 |
| shifts in bacterial communities of eggshells and antimicrobial activities in eggs during incubation in a ground-nesting passerine. | microbial invasion of egg contents is a cause of embryonic death. to counter infection risks, the embryo is protected physically by the eggshell and chemically by antimicrobial proteins. if microbial pressure drives embryo mortality, then females may have evolved, through natural selection, to adapt their immune investment into eggs. although frequently hypothesized, this match between immune allocation and microorganisms has not been explored yet. to examine if correlations between microbes on ... | 2015 | 25880684 |
| pyrethroid-degrading microorganisms and their potential for the bioremediation of contaminated soils: a review. | pyrethroid insecticides have been used to control pests in agriculture, forestry, horticulture, public health and for indoor home use for more than 20 years. because pyrethroids were considered to be a safer alternative to organophosphate pesticides (ops), their applications significantly increased when the use of ops was banned or limited. although, pyrethroids have agricultural benefits, their widespread and continuous use is a major problem as they pollute the terrestrial and aquatic environm ... | 2016 | 27695449 |
| response mechanisms of bacterial degraders to environmental contaminants on the level of cell walls and cytoplasmic membrane. | bacterial strains living in the environment must cope with the toxic compounds originating from humans production. surface bacterial structures, cell wall and cytoplasmic membrane, surround each bacterial cell and create selective barriers between the cell interior and the outside world. they are a first site of contact between the cell and toxic compounds. organic pollutants are able to penetrate into cytoplasmic membrane and affect membrane physiological functions. bacteria had to evolve adapt ... | 2014 | 25057269 |
| ecological significance of microdiversity: coexistence among casing soil bacterial strains through allocation of nutritional resource. | a combination of cultivation-based methods with a molecular biological approach was employed to investigate whether bacteria with identical 16s rrna gene sequences can represent distinct eco- and genotypes. a set of eight bacterial strains wherein three were pseudomonas putida and rest were acinetobacter calcoaceticus, were isolated from casing soils community by conventional plating. these strains had identical 16s rrna gene sequences and represented the dominant phylotype in the plateable frac ... | 2011 | 22282622 |
| diversity of bacteria nesting the plant cover of north sinai deserts, egypt. | north sinai deserts were surveyed for the predominant plant cover and for the culturable bacteria nesting their roots and shoots. among 43 plant species reported, 13 are perennial (e.g. fagonia spp., pancratium spp.) and 30 annuals (e.g. bromus spp., erodium spp.). eleven species possessed rhizo-sheath, e.g. cyperus capitatus, panicum turgidum and trisetaria koelerioides. microbiological analyses demonstrated: the great diversity and richness of associated culturable bacteria, in particular nitr ... | 2012 | 25685397 |
| diversity of bacteria nesting the plant cover of north sinai deserts, egypt. | north sinai deserts were surveyed for the predominant plant cover and for the culturable bacteria nesting their roots and shoots. among 43 plant species reported, 13 are perennial (e.g. fagonia spp., pancratium spp.) and 30 annuals (e.g. bromus spp., erodium spp.). eleven species possessed rhizo-sheath, e.g. cyperus capitatus, panicum turgidum and trisetaria koelerioides. microbiological analyses demonstrated: the great diversity and richness of associated culturable bacteria, in particular nitr ... | 2012 | 25685397 |
| experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes. | the rate at which genome sequencing data is accruing demands enhanced methods for functional annotation and metabolism discovery. solute binding proteins (sbps) facilitate the transport of the first reactant in a metabolic pathway, thereby constraining the regions of chemical space and the chemistries that must be considered for pathway reconstruction. we describe high-throughput protein production and differential scanning fluorimetry platforms, which enabled the screening of 158 sbps against a ... | 2014 | 25540822 |
| experimental strategies for functional annotation and metabolism discovery: targeted screening of solute binding proteins and unbiased panning of metabolomes. | the rate at which genome sequencing data is accruing demands enhanced methods for functional annotation and metabolism discovery. solute binding proteins (sbps) facilitate the transport of the first reactant in a metabolic pathway, thereby constraining the regions of chemical space and the chemistries that must be considered for pathway reconstruction. we describe high-throughput protein production and differential scanning fluorimetry platforms, which enabled the screening of 158 sbps against a ... | 2014 | 25540822 |
| molecular mechanism of nicotine degradation by a newly isolated strain, ochrobactrum sp. strain sjy1. | a newly isolated strain, sjy1, identified as ochrobactrum sp., utilizes nicotine as a sole source of carbon, nitrogen, and energy. strain sjy1 could efficiently degrade nicotine via a variant of the pyridine and pyrrolidine pathways (the vpp pathway), which highlights bacterial metabolic diversity in relation to nicotine degradation. a 97-kbp dna fragment containing six nicotine degradation-related genes was obtained by gap closing from the genome sequence of strain sjy1. three genes, designated ... | 2014 | 25344232 |
| molecular mechanism of nicotine degradation by a newly isolated strain, ochrobactrum sp. strain sjy1. | a newly isolated strain, sjy1, identified as ochrobactrum sp., utilizes nicotine as a sole source of carbon, nitrogen, and energy. strain sjy1 could efficiently degrade nicotine via a variant of the pyridine and pyrrolidine pathways (the vpp pathway), which highlights bacterial metabolic diversity in relation to nicotine degradation. a 97-kbp dna fragment containing six nicotine degradation-related genes was obtained by gap closing from the genome sequence of strain sjy1. three genes, designated ... | 2014 | 25344232 |
| physical, chemical, and biological methods for the removal of arsenic compounds. | arsenic is a toxic metalloid which is widely distributed in nature. it is normally present as arsenate under oxic conditions while arsenite is predominant under reducing condition. the major discharges of arsenic in the environment are mainly due to natural sources such as aquifers and anthropogenic sources. it is known that arsenite salts are more toxic than arsenate as it binds with vicinal thiols in pyruvate dehydrogenase while arsenate inhibits the oxidative phosphorylation process. the comm ... | 2014 | 24696853 |
| correction of sequence-dependent ambiguous bases (ns) from the 454 pyrosequencing system. | pyrosequencing of the 16s ribosomal rna gene (16s) has become one of the most popular methods to assess microbial diversity. pyrosequencing reads containing ambiguous bases (ns) are generally discarded based on the assumptions of their non-sequence-dependent formation and high error rates. however, taxonomic composition differed by removal of reads with ns. we determined whether ns from pyrosequencing occur in a sequence-dependent manner. our reads and the corresponding flow value data revealed ... | 2014 | 24464999 |
| prevalence and antimicrobial resistance of microbes causing bloodstream infections in unguja, zanzibar. | bloodstream infections (bsi) are frequent and cause high case-fatality rates. urgent antibiotic treatment can save patients' lives, but antibiotic resistance can render antibiotic therapy futile. this study is the first to collect epidemiological data on bsi from unguja, zanzibar. | 2015 | 26700032 |
| phenotypic and phylogenetic identification of coliform bacteria obtained using 12 coliform methods approved by the u.s. environmental protection agency. | the current definition of coliform bacteria is method dependent, and when different culture-based methods are used, discrepancies in results can occur and affect the accuracy of identification of true coliforms. this study used an alternative approach to the identification of true coliforms by combining the phenotypic traits of the coliform isolates and the phylogenetic affiliation of 16s rrna gene sequences with the use of lacz and uida genes. a collection of 1,404 isolates detected by 12 u.s. ... | 2015 | 26116679 |
| conjunctival flora of clinically normal and diseased turtles and tortoises. | in captive breed turtles and tortoises conjunctival disease is common. our aim was to investigate the bacterial and fungal flora present in the eyes of healthy and pathological chelonians and to compare findings in turtles with those in tortoises. | 2015 | 25889261 |
| depth and well type related to groundwater microbiological contamination. | use of groundwater from private wells in households has increased considerably, owing to a better cost/benefit ratio than that of water provided by local utilities for a fee. however, this water is usually untreated, which makes it a vehicle for diseases. thus, monitoring this water is necessary to ensure its integrity and quality. we aimed to evaluate the physical, chemical, and microbiological parameters of untreated groundwater drawn from different types of wells, and the antimicrobial suscep ... | 2016 | 27775681 |
| genomics of high molecular weight plasmids isolated from an on-farm biopurification system. | the use of biopurification systems (bps) constitutes an efficient strategy to eliminate pesticides from polluted wastewaters from farm activities. bps environments contain a high microbial density and diversity facilitating the exchange of information among bacteria, mediated by mobile genetic elements (mges), which play a key role in bacterial adaptation and evolution in such environments. here we sequenced and characterized high-molecular-weight plasmids from a bacterial collection of an on-fa ... | 2016 | 27321040 |
| identification of acquired antimicrobial resistance genes. | identification of antimicrobial resistance genes is important for understanding the underlying mechanisms and the epidemiology of antimicrobial resistance. as the costs of whole-genome sequencing (wgs) continue to decline, it becomes increasingly available in routine diagnostic laboratories and is anticipated to substitute traditional methods for resistance gene identification. thus, the current challenge is to extract the relevant information from the large amount of generated data. | 2012 | 22782487 |
| the still mysterious roles of cysteine-containing glutathione transferases in plants. | glutathione transferases (gsts) represent a widespread multigenic enzyme family able to modify a broad range of molecules. these notably include secondary metabolites and exogenous substrates often referred to as xenobiotics, usually for their detoxification, subsequent transport or export. to achieve this, these enzymes can bind non-substrate ligands (ligandin function) and/or catalyze the conjugation of glutathione onto the targeted molecules, the latter activity being exhibited by gsts having ... | 2014 | 25191271 |
| unconventional serine proteases: variations on the catalytic ser/his/asp triad configuration. | serine proteases comprise nearly one-third of all known proteases identified to date and play crucial roles in a wide variety of cellular as well as extracellular functions, including the process of blood clotting, protein digestion, cell signaling, inflammation, and protein processing. their hallmark is that they contain the so-called "classical" catalytic ser/his/asp triad. although the classical serine proteases are the most widespread in nature, there exist a variety of "nonclassical" serine ... | 2008 | 18824507 |
| the shared antibiotic resistome of soil bacteria and human pathogens. | soil microbiota represent one of the ancient evolutionary origins of antibiotic resistance and have been proposed as a reservoir of resistance genes available for exchange with clinical pathogens. using a high-throughput functional metagenomic approach in conjunction with a pipeline for the de novo assembly of short-read sequence data from functional selections (termed parfums), we provide evidence for recent exchange of antibiotic resistance genes between environmental bacteria and clinical pat ... | 2012 | 22936781 |
| culture-independent investigation of the microbiome associated with the nematode acrobeloides maximus. | symbioses between metazoans and microbes are widespread and vital to many ecosystems. recent work with several nematode species has suggested that strong associations with microbial symbionts may also be common among members of this phylu. in this work we explore possible symbiosis between bacteria and the free living soil bacteriovorous nematode acrobeloides maximus. | 2013 | 23894287 |
| degradation of 4-n-nonylphenol under nitrate reducing conditions. | nonylphenol (np) is an endocrine disruptor present as a pollutant in river sediment. biodegradation of np can reduce its toxicological risk. as sediments are mainly anaerobic, degradation of linear (4-n-np) and branched nonylphenol (tnp) was studied under methanogenic, sulphate reducing and denitrifying conditions in np polluted river sediment. anaerobic bioconversion was observed only for linear np under denitrifying conditions. the microbial population involved herein was further studied by en ... | 2010 | 20640878 |
| degradation of 4-n-nonylphenol under nitrate reducing conditions. | nonylphenol (np) is an endocrine disruptor present as a pollutant in river sediment. biodegradation of np can reduce its toxicological risk. as sediments are mainly anaerobic, degradation of linear (4-n-np) and branched nonylphenol (tnp) was studied under methanogenic, sulphate reducing and denitrifying conditions in np polluted river sediment. anaerobic bioconversion was observed only for linear np under denitrifying conditions. the microbial population involved herein was further studied by en ... | 2010 | 20640878 |
| chronic postoperative endophthalmitis: a review of clinical characteristics, microbiology, treatment strategies, and outcomes. | chronic postoperative endophthalmitis (cpe) is a delayed infectious intraocular inflammation process that occurs more than six weeks after ocular surgery and frequently masquerades as autoimmune uveitis. these cases are at risk of delayed diagnosis and erroneous long-term treatment with corticosteroids. this paper aims to review the epidemiology, microbiology, clinical characteristics, diagnosis, management strategies, and outcome of chronic postoperative endophthalmitis. the incidence of cpe is ... | 2012 | 22550607 |
| antibacterial properties of silver nanoparticles synthesized by marine ochrobactrum sp. | metal nanoparticle synthesis is an interesting area in nanotechnology due to their remarkable optical, magnetic, electrical, catalytic and biomedical properties, but there needs to develop clean, non-toxic and environmental friendly methods for the synthesis and assembly of nanoparticles. biological agents in the form of microbes have emerged up as efficient candidates for nanoparticle synthesis due to their extreme versatility to synthesize diverse nanoparticles with varying size and shape. in ... | 2014 | 25763025 |
| antibacterial properties of silver nanoparticles synthesized by marine ochrobactrum sp. | metal nanoparticle synthesis is an interesting area in nanotechnology due to their remarkable optical, magnetic, electrical, catalytic and biomedical properties, but there needs to develop clean, non-toxic and environmental friendly methods for the synthesis and assembly of nanoparticles. biological agents in the form of microbes have emerged up as efficient candidates for nanoparticle synthesis due to their extreme versatility to synthesize diverse nanoparticles with varying size and shape. in ... | 2014 | 25763025 |
| utilization of glyphosate as phosphate source: biochemistry and genetics of bacterial carbon-phosphorus lyase. | after several decades of use of glyphosate, the active ingredient in weed killers such as roundup, in fields, forests, and gardens, the biochemical pathway of transformation of glyphosate phosphorus to a useful phosphorus source for microorganisms has been disclosed. glyphosate is a member of a large group of chemicals, phosphonic acids or phosphonates, which are characterized by a carbon-phosphorus bond. this is in contrast to the general phosphorus compounds utilized and metabolized by microor ... | 2014 | 24600043 |
| halotolerant rhizobacteria promote growth and enhance salinity tolerance in peanut. | use of plant growth promoting rhizobacteria (pgpr) is a promising strategy to improve the crop production under optimal or sub-optimal conditions. in the present study, five diazotrophic salt tolerant bacteria were isolated from the roots of a halophyte, arthrocnemum indicum. the isolates were partially characterized in vitro for plant growth promoting traits and evaluated for their potential to promote growth and enhanced salt tolerance in peanut. the 16s rrna gene sequence homology indicated t ... | 2016 | 27790198 |
| semiquantitative analysis of clinical heat stress in clostridium difficile strain 630 using a gelc/ms workflow with empai quantitation. | clostridium difficile is considered to be the most frequent cause of infectious bacterial diarrhoea in hospitals worldwide yet its adaptive ability remains relatively uncharacterised. here, we used gelc/ms and the exponentially modified protein abundance index (empai) calculation to determine proteomic changes in response to a clinically relevant heat stress. reproducibility between both biological and technical replicates was good, and a 37°c proteome of 224 proteins was complemented by a 41°c ... | 2014 | 24586458 |
| bacteria may have multiple replication origins. | | 2015 | 25941523 |
| dissecting the metal selectivity of merr monovalent metal ion sensors in salmonella. | two homologous transcription factors, cuer and gols, that belong to the merr metalloregulatory family are responsible for salmonella cu and au sensing and resistance, respectively. they share similarities not only in their sequences, but also in their target transcription binding sites. while cuer responds similarly to au, ag, or cu to induce the expression of its target genes, gols shows higher activation by au than by ag or cu. we showed that the ability of gols to distinguish au from cu resid ... | 2013 | 23645605 |
| structural differences in gut bacteria communities in developmental stages of natural populations of lutzomyia evansi from colombia's caribbean coast. | lutzomyia evansi, a phlebotomine insect endemic to colombia's caribbean coast, is considered to be the main vector of visceral and cutaneous leishmaniasis in the region. although insects of this species can harbor pathogenic and non-pathogenic microorganisms in their intestinal microbiota, there is little information available about the diversity of gut bacteria present in lutzomyia evansi. in this study, conventional microbiological methods and molecular tools were used to assess the compositio ... | 2016 | 27618991 |
| niches, population structure and genome reduction in ochrobactrum intermedium: clues to technology-driven emergence of pathogens. | ochrobactrum intermedium is considered as an emerging human environmental opportunistic pathogen with mild virulence. the distribution of isolates and sequences described in literature and databases showed frequent association with human beings and polluted environments. as population structures are related to bacterial lifestyles, we investigated by multi-locus approach the genetic structure of a population of 65 isolates representative of the known natural distribution of o. intermedium. the p ... | 2014 | 24465379 |
| proteomic profiling of rhizobium tropici prf 81: identification of conserved and specific responses to heat stress. | rhizobium tropici strain prf 81 (= semia 4080) has been used in commercial inoculants for application to common-bean crops in brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of n-fertilizers. the objective of our study was to obtain an overview of adaptive responses to heat stress of strain prf 81, by analyzing differenti ... | 2012 | 22647150 |
| microbial glycolipoprotein-capped silver nanoparticles as emerging antibacterial agents against cholera. | with the increased number of cholera outbreaks and emergence of multidrug resistance in vibrio cholerae strains it has become necessary for the scientific community to devise and develop novel therapeutic approaches against cholera. recent studies have indicated plausibility of therapeutic application of metal nano-materials. among these, silver nanoparticles (agnps) have emerged as a potential antimicrobial agent to combat infectious diseases. at present nanoparticles are mostly produced using ... | 2016 | 26829922 |
| site-specific bacterial chromosome engineering: φc31 integrase mediated cassette exchange (imce). | the bacterial chromosome may be used to stably maintain foreign dna in the mega-base range. integration into the chromosome circumvents issues such as plasmid replication, plasmid stability, plasmid incompatibility, and plasmid copy number variance. this method uses the site-specific integrase from the streptomyces phage (φ) c31. the φc31 integrase catalyzes a direct recombination between two specific dna sites: attb and attp (34 and 39 bp, respectively). this recombination is stable and does no ... | 2012 | 22453661 |
| isolation and characterization of a crude oil degrading bacteria from formation water: comparative genomic analysis of environmental ochrobactrum intermedium isolate versus clinical strains. | in this study, we isolated an environmental clone of ochrobactrum intermedium, strain 2745-2, from the formation water of changqing oilfield in shanxi, china, which can degrade crude oil. strain 2745-2 is aerobic and rod-shaped with optimum growth at 42 °c and ph 5.5. we sequenced the genome and found a single chromosome of 4 800 175 bp, with a g+c content of 57.63%. sixty rnas and 4737 protein-coding genes were identified: many of the genes are responsible for the degradation, emulsification, a ... | 2015 | 26465134 |
| specific microbial attachment to root knot nematodes in suppressive soil. | understanding the interactions of plant-parasitic nematodes with antagonistic soil microbes could provide opportunities for novel crop protection strategies. three arable soils were investigated for their suppressiveness against the root knot nematode meloidogyne hapla. for all three soils, m. hapla developed significantly fewer galls, egg masses, and eggs on tomato plants in unsterilized than in sterilized infested soil. egg numbers were reduced by up to 93%. this suggested suppression by soil ... | 2014 | 24532076 |
| fim-1, a new acquired metallo-β-lactamase from a pseudomonas aeruginosa clinical isolate from italy. | acquired metallo-β-lactamases (mbls) are resistance determinants of increasing clinical importance in gram-negative bacterial pathogens, which confer a broad-spectrum β-lactam resistance, including carbapenems. several such enzymes have been described since the 1990s. in the present study, a novel acquired mbl, named fim-1, was identified and characterized. the bla(fim-1) gene was cloned from a multidrug-resistant pseudomonas aeruginosa clinical isolate (fi-14/157) cultured from a patient with a ... | 2013 | 23114762 |
| controlled clinical comparison of versatrek and bact/alert blood culture systems. | to assess the relative yields in automated microbial detection systems of bacteria and yeasts isolated from the blood of adult patients with suspected sepsis, we compared the new versatrek system (vti) (trek diagnostic systems, cleveland, oh) to the bact/alert 3d system (3d) (biomérieux, inc., durham, nc). identical protocols were followed for the two systems. paired aerobic (redox 1) and anaerobic (redox 2) vti media were compared with standard aerobic (sa) and anaerobic (sn) 3d media; each of ... | 2006 | 17122016 |
| controlled clinical comparison of versatrek and bact/alert blood culture systems. | to assess the relative yields in automated microbial detection systems of bacteria and yeasts isolated from the blood of adult patients with suspected sepsis, we compared the new versatrek system (vti) (trek diagnostic systems, cleveland, oh) to the bact/alert 3d system (3d) (biomérieux, inc., durham, nc). identical protocols were followed for the two systems. paired aerobic (redox 1) and anaerobic (redox 2) vti media were compared with standard aerobic (sa) and anaerobic (sn) 3d media; each of ... | 2006 | 17122016 |
| evaluation of the diversilab typing method in a multicenter study assessing horizontal spread of highly resistant gram-negative rods. | the worldwide prevalence of highly resistant gram-negative rods (hr-gnr) is increasing rapidly. reliable typing methods are needed to detect and control outbreaks and to monitor the effectiveness of infection control programs in endemic situations. in this study, we investigated the performance of the diversilab typing method in comparison with the amplified fragment length polymorphism (aflp) typing method. six hundred fifty-three hr-gnr isolates, which were obtained during a 6-month prospectiv ... | 2011 | 21865432 |
| plasmid-determined ampc-type beta-lactamases. | | 2002 | 11751104 |
| advances in marine microbial symbionts in the china sea and related pharmaceutical metabolites. | marine animals and plants such as sponges, sea squirts, corals, worms and algae host diverse and abundant symbiotic microorganisms. marine microbial symbionts are possible the true producers or take part in the biosynthesis of some bioactive marine natural products isolated from the marine organism hosts. investigation of the pharmaceutical metabolites may reveal the biosynthesis mechanisms of related natural products and solve the current problem of supply limitation in marine drug development. ... | 2009 | 19597576 |
| a novel a3 group aconitase tolerates oxidation and nitric oxide. | achromobacter denitrificans yd35 is an no2 (-)-tolerant bacterium that expresses the aconitase genes acna3, acna4, and acnb, of which acna3 is essential for growth tolerance against 100 mm no2 (-). atmospheric oxygen inactivated acna3 at a rate of 1.6 × 10(-3) min(-1), which was 2.7- and 37-fold lower compared with acna4 and acnb, respectively. stoichiometric titration showed that the [4fe-4s](2+) cluster of acna3 was more stable against oxidative inactivation by ferricyanide than that of acna4. ... | 2014 | 25477516 |
| a novel a3 group aconitase tolerates oxidation and nitric oxide. | achromobacter denitrificans yd35 is an no2 (-)-tolerant bacterium that expresses the aconitase genes acna3, acna4, and acnb, of which acna3 is essential for growth tolerance against 100 mm no2 (-). atmospheric oxygen inactivated acna3 at a rate of 1.6 × 10(-3) min(-1), which was 2.7- and 37-fold lower compared with acna4 and acnb, respectively. stoichiometric titration showed that the [4fe-4s](2+) cluster of acna3 was more stable against oxidative inactivation by ferricyanide than that of acna4. ... | 2014 | 25477516 |
| isolation and characterisation of crude oil sludge degrading bacteria. | the use of microorganisms in remediating environmental contaminants such as crude oil sludge has become a promising technique owing to its economy and the fact it is environmentally friendly. polycyclic aromatic hydrocarbons (pahs), as the major components of oil sludge, are hydrophobic and recalcitrant. an important way of enhancing the rate of pah desorption is to compost crude oil sludge by incorporating commercial surfactants, thereby making them available for microbial degradation. in this ... | 2016 | 27933233 |
| validation of the automated reading and incubation system with sensititre plates for antimicrobial susceptibility testing. | the present study compared the antimicrobial susceptibility testing (ast) results generated by the automated incubation and reading system (aris) with custom sensititre plates (trek diagnostic systems, cleveland, ohio) and microscan pc10 gp and numic10 gn plates interpreted with the walkaway-96 system (dade behring, west sacramento, calif.) for gram-positive (gp) and gram-negative (gn) organisms as part of an in-house validation. a total of 326 isolates (3,689 antimicrobial agent-organism combin ... | 2003 | 12734233 |
| a novel beta-peptidyl aminopeptidase (bapa) from strain 3-2w4 cleaves peptide bonds of synthetic beta-tri- and beta-dipeptides. | a novel bacterial strain that was capable of growing on the beta-tripeptide h-betahval-betahala-betahleu-oh as the sole carbon and nitrogen source was isolated from an enrichment culture. on the basis of physiological characterization, partial 16s rrna sequencing, and fatty acid analysis, strain 3-2w4 was identified as a member of the family sphingomonadaceae. growth on the beta-tripeptide and the beta-dipeptide h-betahala-betahleu-oh was observed, and emerging metabolites were characterized. sm ... | 2005 | 16109932 |
| microbial proteomics: a mass spectrometry primer for biologists. | it is now more than 10 years since the publication of the first microbial genome sequence and science is now moving towards a post genomic era with transcriptomics and proteomics offering insights into cellular processes and function. the ability to assess the entire protein network of a cell at a given spatial or temporal point will have a profound effect upon microbial science as the function of proteins is inextricably linked to phenotype. whilst such a situation is still beyond current techn ... | 2007 | 17697372 |
| biodegradability of commercial and weathered diesel oils. | this work aimed to evaluate the capability of different microorganisms to degrade commercial diesel oil in comparison to a weathered diesel oil collected from the groundwater at a petrol station. two microbiological methods were used for the biodegradability assessment: the technique based on the redox indicator 2,6 -dichlorophenol indophenol (dcpip) and soil respirometric experiments using biometer flasks. in the former we tested the bacterial cultures staphylococcus hominis, kocuria palustris, ... | 2008 | 24031193 |
| characterization of a monothiol glutaredoxin encoded by chlorella virus pbcv-1. | annotation of the 330-kb chlorella virus pbcv-1 genome identified a 237 nucleotide gene (a438l) that codes for a protein with approximately 35% amino acid identity to glutaredoxins (grx) found in other organisms. the pbcv-1 protein resembles classical grxs in both size (9 kda) and location of the active site (n-terminus). however, the pbcv-1 grx is unusual because it contains a monothiol active site (cpys) rather than the typical dithiol active site (cpyc). to examine this unique active site, fo ... | 2009 | 19697117 |
| generation, analysis and functional annotation of expressed sequence tags from the sheepshead minnow (cyprinodon variegatus). | sheepshead minnow (cyprinodon variegatus) are small fish capable of withstanding exposure to very low levels of dissolved oxygen, as well as extreme temperatures and salinities. it is an important model in understanding the impacts and biological response to hypoxia and co-occurring compounding stressors such as polycyclic aromatic hydrocarbons, endocrine disrupting chemicals, metals and herbicides. here, we initiated a project to sequence and analyze over 10,000 ests generated from the sheepshe ... | 2010 | 21047385 |
| bioinformatic analysis, molecular modeling of role of lys65 residue in catalytic triad of d-aminopeptidase from ochrobactrum anthropi. | a bioinformatic and phylogenetic study has been performed on a family of penicillin-binding proteins including d-aminopeptidases, d-amino acid amidases, dd-carboxypeptidases, and β -lactamases. significant homology between d-aminopeptidase from ochrobactrum anthropi and other members of the family has been shown and a number of conserved residues identified as s62, k65, y153, n155, h287, and g289. three of those (ser62, lys65, and tyr153) form a catalytic triangle - the proton relay system that ... | 2010 | 22649642 |
| herbicide-resistant crops: utilities and limitations for herbicide-resistant weed management. | since 1996, genetically modified herbicide-resistant (hr) crops, particularly glyphosate-resistant (gr) crops, have transformed the tactics that corn, soybean, and cotton growers use to manage weeds. the use of gr crops continues to grow, but weeds are adapting to the common practice of using only glyphosate to control weeds. growers using only a single mode of action to manage weeds need to change to a more diverse array of herbicidal, mechanical, and cultural practices to maintain the effectiv ... | 2010 | 20586458 |
| herbicide-resistant crops: utilities and limitations for herbicide-resistant weed management. | since 1996, genetically modified herbicide-resistant (hr) crops, particularly glyphosate-resistant (gr) crops, have transformed the tactics that corn, soybean, and cotton growers use to manage weeds. the use of gr crops continues to grow, but weeds are adapting to the common practice of using only glyphosate to control weeds. growers using only a single mode of action to manage weeds need to change to a more diverse array of herbicidal, mechanical, and cultural practices to maintain the effectiv ... | 2010 | 20586458 |
| ochrobactrum anthropi septic arthritis: case report and implications in orthopedic infections. | ochrobactrum anthropi is a rare cause of orthopedic infections. we report the second case of ochrobactrum anthropi septic arthritis in the literature. our case highlights the ability of ochrobactrum anthropi to cause septic arthritis and its relevance in the field of orthopedic infections. | 2010 | 24470885 |
| functional annotation and three-dimensional structure of an incorrectly annotated dihydroorotase from cog3964 in the amidohydrolase superfamily. | the substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. this group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. atu3266 from agrobacterium tumefaciens c58 and oant2987 from ochrobactrum anthropi atcc 49188 were found to catalyze the hydrolysis of acetyl-(r)-mandelate and similar esters with values of k(cat)/k(m) that exceed 10(5) m(-1) s(-1). these enzymes do not catalyze ... | 2012 | 23214420 |
| functional annotation and three-dimensional structure of an incorrectly annotated dihydroorotase from cog3964 in the amidohydrolase superfamily. | the substrate specificities of two incorrectly annotated enzymes belonging to cog3964 from the amidohydrolase superfamily were determined. this group of enzymes are currently misannotated as either dihydroorotases or adenine deaminases. atu3266 from agrobacterium tumefaciens c58 and oant2987 from ochrobactrum anthropi atcc 49188 were found to catalyze the hydrolysis of acetyl-(r)-mandelate and similar esters with values of k(cat)/k(m) that exceed 10(5) m(-1) s(-1). these enzymes do not catalyze ... | 2012 | 23214420 |
| peritonitis and technique failure caused by roseomonas mucosa in an adolescent infected with hiv on continuous cycling peritoneal dialysis. | we report the first case of peritonitis caused by roseomonas mucosa which led to technique failure in an adolescent patient with hiv receiving peritoneal dialysis. identification of the causative organism by 16s rrna gene sequencing and phylogenetic analysis is described. | 2012 | 22933600 |
| bacterial selection during the formation of early-stage aerobic granules in wastewater treatment systems operated under wash-out dynamics. | aerobic granular sludge is attractive for high-rate biological wastewater treatment. biomass wash-out conditions stimulate the formation of aerobic granules. deteriorated performances in biomass settling and nutrient removal during start-up have however often been reported. the effect of wash-out dynamics was investigated on bacterial selection, biomass settling behavior, and metabolic activities during the formation of early-stage granules from activated sludge of two wastewater treatment plant ... | 2012 | 22993513 |
| raman spectroscopy as a potential tool for detection of brucella spp. in milk. | detection of brucella, causing brucellosis, is very challenging, since the applied techniques are mostly time-demanding and not standardized. while the common detection system relies on the cultivation of the bacteria, further classical typing up to the biotype level is mostly based on phenotypic or genotypic characteristics. the results of genotyping do not always fit the existing taxonomy, and misidentifications between genetically closely related genera cannot be avoided. this situation gets ... | 2012 | 22660699 |
| biodiversity of the oleaginous microorganisms in tibetan plateau. | microbial lipids, which are also known as single cell oils (sco), are produced by oleaginous microorganisms including oleaginous bacteria, yeast, fungus and algae through converting carbohydrates into lipids under certain conditions. due to its unique environment having extremely low temperature and anoxia, the tibetan plateau is amongst the regions with numerous rare ecotypes such as arid desert, salt marsh, alpine permafrost, hot spring, and lawn. by using a rapid, convenient screening method, ... | 2012 | 24031873 |
| isolation of potentially novel brucella spp. from frogs. | bacterial isolates from frogs were phenotypically identified as ochrobactrum anthropi, but 16s rrna sequencing showed up to 100% identity with brucella inopinata. further analysis of reca, omp2a, omp2b, bcsp31, and is711 and multilocus sequence analysis (mlsa) verified a close relationship with brucella, suggesting the isolates may actually represent novel members of this growing genus of zoonotic pathogens. | 2012 | 22407680 |
| identification, crystallization and preliminary x-ray diffraction analysis of esterase a from caulobacter crescentus cb15, a family viii lipolytic enzyme. | the structures and functions of family viii lipolytic enzymes, which have moderate sequence identity to class c β-lactamases and penicillin-binding proteins, are largely unknown. here, the x-ray crystallographic study of a family viii esterase from caulobacter crescentus cb15 (ccesta) is described. sequence analysis revealed that ccesta has a conserved serine residue within the s-x-x-k motif which acts as a catalytic nucleophile. recombinant protein containing an n-terminal his tag was expressed ... | 2012 | 22691788 |
| a glutathione transferase from agrobacterium tumefaciens reveals a novel class of bacterial gst superfamily. | in the present work, we report a novel class of glutathione transferases (gsts) originated from the pathogenic soil bacterium agrobacterium tumefaciens c58, with structural and catalytic properties not observed previously in prokaryotic and eukaryotic gst isoenzymes. a gst-like sequence from a. tumefaciens c58 (atu3701) with low similarity to other characterized gst family of enzymes was identified. phylogenetic analysis showed that it belongs to a distinct gst class not previously described and ... | 2012 | 22496785 |
| escherichia coli atcc 8739 adapts to the presence of sodium chloride, monosodium glutamate, and benzoic acid after extended culture. | escherichia coli is commonly found in intestine of human, and any changes in their adaptation or evolution may affect the human body. the relationship between e. coli and food additives is less studied as compared to antibiotics. e. coli within our human gut are consistently interacting with the food additives; thus, it is important to investigate this relationship. in this paper, we observed the evolution of e. coli cultured in different concentration of food additives (sodium chloride, benzoic ... | 2012 | 23724334 |
| insights into the transposable mobilome of paracoccus spp. (alphaproteobacteria). | several trap plasmids (enabling positive selection of transposition events) were used to identify a pool of functional transposable elements (tes) residing in bacteria of the genus paracoccus (alphaproteobacteria). complex analysis of 25 strains representing 20 species of this genus led to the capture and characterization of (i) 37 insertion sequences (iss) representing 9 is families (is3, is5, is6, is21, is66, is256, is1182, is1380 and is1634), (ii) a composite transposon tn6097 generated by tw ... | 2012 | 22359677 |
| immobilization of anode-attached microbes in a microbial fuel cell. | current-generating (exoelectrogenic) bacteria in bioelectrochemical systems (bess) may not be culturable using standard in vitro agar-plating techniques, making isolation of new microbes a challenge. more in vivo like conditions are needed where bacteria can be grown and directly isolated on an electrode. while colonies can be developed from single cells on an electrode, the cells must be immobilized after being placed on the surface. here we present a proof-of-concept immobilization approach th ... | 2012 | 22214379 |
| a review of 11 years of stenotrophomonas maltophilia blood isolates at a tertiary care institute in canada. | stenotrophomonas maltophilia has emerged as a significant nosocomial pathogen with increasing resistance to trimethoprim/sulphamethoxazole (tmp/smx), the current drug of choice for treatment. | 2012 | 24294268 |