| plant phenolic compounds induce expression of the agrobacterium tumefaciens loci needed for virulence. | the virulence loci of agrobacterium tumefaciens are a set of linked transcriptional units that play an essential role in the early stages of plant tumorigenesis. these loci are induced upon cocultivation of the bacteria with plant cells. seven phenolic compounds that are widely distributed among the angiosperm plants--catechol, gallic acid, pyrogallic acid, p-hydroxybenzoic acid, protocatechuic acid, beta-resorcylic acid, and vanillin--are able to induce the expression of the virulence loci. the ... | 1986 | 3085219 |
| genetic analysis of mannityl opine catabolism in octopine-type agrobacterium tumefaciens strain 15955. | the genetic organization of functions responsible for mannityl opine catabolism of the ti plasmid of agrobacterium tumefaciens strain 1,5955 was investigated. a partial hindiii digest of pti1,5955 was cloned into a broad host range cosmid and the clones obtained were tested for ability to confer mannityl opine degradation upon agrobacterium. inserts containing genes for catabolism of mannopinic acid, mannopine, agropine, and agropinic acid were obtained, spanning a segment of 43 kb on the ti pla ... | 1987 | 3112522 |
| ti plasmid-specified chemotaxis of agrobacterium tumefaciens c58c1 toward vir-inducing phenolic compounds and soluble factors from monocotyledonous and dicotyledonous plants. | twelve phenolic compounds with related structures were analyzed for their ability to act as chemoattractants for agrobacterium tumefaciens c58c1 and as inducers of the ti plasmid virulence operons. the results divided the phenolic compounds into three groups: compounds that act as strong vir inducers and are chemoattractants for a. tumefaciens c58c1 harboring the nopaline ti plasmid pdub1003 delta 31, but not the isogenic cured strain; compounds that are at best weak vir inducers and are weak ch ... | 1988 | 3410827 |
| right-hand border regions of octopine t-dna are recognized by rna polymerase of agrobacterium as well as by vird1 and vird2 proteins. | the t-dna of octopine ti plasmid of agrobacterium tumefaciens contains tl- and tr-dna regions each bounded by 25 base-pair-repeats (designated a, b, c and d from left to right). short dna segments containing the borders b, c and d were found to function as promoter when placed in the rightward orientation upstream of promoter-less lacz. promoter consensus sequence of agrobacterium were found within these border repeats and in their adjacent regions. the expression of lacz was low when the segmen ... | 1988 | 3412897 |
| escherichia coli lacz gene as a biochemical and histochemical marker in plant cells. | several lacz chimeric genes were constructed by fusing the truncated lacz sequence of escherichia coli to n-terminal sequences of few other genes. promoters used to direct expression of the chimeric genes were the promoter for 35s rna of cauliflower mosaic virus (p35s) as well as those of the small subunit gene of ribulose bisphosphate carboxylase and the octopine synthase gene. these constructs were introduced into tobacco cells using a ti plasmid of agrobacterium tumefaciens, and beta-galactos ... | 1988 | 3138164 |
| transfer of the ti plasmid from agrobacterium tumefaciens into escherichia coli cells. | we have screened strains of agrobacterium tumefaciens for spontaneous mutants showing constitutive transfer of the nopaline ti plasmid ptic58 during conjugation. the ti plasmid derivatives obtained could be transferred not only to a. tumefaciens but also to e. coli cells. the ti plasmid cannot survive as a freely replicating plasmid in e. coli, but it can occasionally integrate into the e. coli chromosome. however, insertion in tandem of plasmids carrying fd replication origins (pfd plasmids) in ... | 1988 | 3049936 |
| characterization of agrobacterium tumefaciens virulence proteins induced by the plant factor acetosyringone. | the ti plasmid virulence (vir) loci encode functions essential for the transfer of the t-dna element from agrobacterium tumefaciens to plant cells. the expression of these loci is specifically signaled by plant phenolics such as acetosyringone. here, we characterize the protein products that are induced in agrobacterium grown in the presence of acetosyringone. more than 10 to 15 proteins are induced in strains harboring different ti plasmids. two general classes of acetosyringone-induced protein ... | 1987 | 3430596 |
| design and construction of a versatile system for the expression of foreign genes in plants. | we have built a series of vectors to allow the constitutive or light-regulated expression of foreign genes in plants. these vectors carry expression cassettes consisting of either the cauliflower mosaic virus 35s promoter or the pea rbcs-e9 promoter, a multiple cloning site derived from m13um20, and the rbcs-e9 polyadenylation site. these cassettes have been incorporated into pbr322-based or rk2-based replicons to facilitate direct dna uptake or agrobacterium tumefaciens-mediated gene transfer. ... | 1987 | 3443303 |
| the relationship between glucose transport and the production of succinoglucan exopolysaccharide by agrobacterium radiobacter. | agrobacterium radiobacter ncib 11883 was grown in ammonia-limited continuous culture at low dilution rate with glucose as the carbon source. under these conditions the organism produced an extracellular succinoglucan polysaccharide and transported glucose using the same periplasmic glucose-binding proteins (gbp1 and gbp2) as during glucose-limited growth. transition from glucose- to ammonia-limited growth was accompanied by a very rapid decrease in glucose uptake capacity, whereas the glucose-bi ... | 1988 | 3269387 |
| chemotaxis of rhizobium meliloti to the plant flavone luteolin requires functional nodulation genes. | luteolin is a phenolic compound from plants that acts as a potent and specific inducer of nodabc gene expression in rhizobium meliloti. we have found that r. meliloti rcr2011 exhibits positive chemotaxis towards luteolin. a maximum chemotactic response was observed at 10(-8) m. two closely related flavonoids, naringenin and apigenin, were not chemoattractants. the presence of naringenin but not apigenin abolished chemotaxis of r. meliloti towards luteolin. a large deletion in the nif-nod region ... | 1988 | 3384804 |
| characterization of the virb operon from an agrobacterium tumefaciens ti plasmid. | the virulence genes of the agrobacterium tumefaciens ti plasmid are grouped into six transcription units and direct the transfer of t-dna into plant cells. we report here the nucleotide sequence of the largest vir operon, virb, from the ti plasmid ptia6nc. this operon contains 11 open reading frames, 7 of which show evidence of translational coupling. trpe::virb gene fusions were used to confirm the reading frames of genes virb2, 4, 5, 6, 7, 8, 10, and 11. in addition, the native gene products o ... | 1988 | 3281947 |
| t-dna and opine synthetic loci in tumors incited by agrobacterium tumefaciens a281 on soybean and alfalfa plants. | we report here the molecular characterization of transferred dna (t-dna) in leguminous tumors incited by agrobacterium tumefaciens a281 harboring the tumor-inducing plasmid ptibo542. the t-dna is composed of two regions named tl (left portion)-dna and tr (right portion)-dna, in accordance with the nomenclature for the octopine strains. tl-dna is defined by several internal hindiii restriction fragments totaling 10.8 kilobase pairs (kbp) in uncloned soybean and alfalfa tumors. alfalfa tumor dna m ... | 1986 | 3023301 |
| association of the vird2 protein with the 5' end of t strands in agrobacterium tumefaciens. | the soil bacterium agrobacterium tumefaciens can incite tumors in many dicotyledonous plants by transferring a portion (t-dna) of its ti plasmid into susceptible plant cells. the t-dna is flanked by border sequences that serve as recognition sites for specific cleavage by an endonuclease that comprises two vird-encoded proteins (vird1 and vird2). after cleavage, both double-stranded, nicked t-dna molecules and single-stranded t-dna molecules (t strands) were present. we have determined that a pr ... | 1988 | 3403506 |
| biosynthesis and degradation of nodule-specific rhizobium loti compounds in lotus nodules. | two nodule-specific rhizobium loti compounds were identified in lotus tenuis and lotus pedunculatus nodules induced by strain nzp2037. one, a silver nitrate-positive cation called rhizolotine, has been characterized as the riboside of a novel alpha-hydroxyimino acid containing a 1,4,5,6-tetrahydropyrimidine ring (g. j. shaw, r. d. wilson, g. a. lane, l. d. kennedy, d. b. scott, and g. j. gainsford, j. chem. soc. chem. commun., p. 180-181, 1986), and the other, yellow-1, stains yellow with ninhyd ... | 1987 | 3025173 |
| recognition of individual strains of fast-growing rhizobia by using profiles of membrane proteins and lipopolysaccharides. | membrane protein and lipopolysaccharide profiles of rhizobium leguminosarum (biovars viciae, trifolii, and phaseoli), r. meliloti, and agrobacterium tumefaciens strains were analyzed and compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. differences in one or both profiles allowed us to distinguish all 18 r. leguminosarum strains tested in this study from each other. | 1988 | 3403513 |
| characterization of nonattaching mutants of agrobacterium tumefaciens. | the first step in tumor formation by agrobacterium tumefaciens is the site-specific binding of the bacteria to plant host cells. transposon mutants of the bacteria which fail to attach to carrot suspension culture cells were isolated. these mutants showed no significant attachment to carrot cells with either microscopic or viable cell count assays of bacterial binding. the nonattaching mutants were all avirulent. when revertants of the mutants were obtained by enriching for bacteria which do bin ... | 1987 | 3025176 |
| [transfer of hybrid plasmids prp1.2::mini-mu into agrobacterium and rhizobium cells]. | the study is devoted to determination of bacteriophage mu genome regions responsible for transfer limitation and instability of the plasmids in cells of strains of practically important microorganisms. with this aim in view, we determined the frequency of transfer into agrobacterium tumefaciens and rhizobium meliloti cells of plasmids with mini-mu phages carrying previously constructed deletions of various lengths. sharp decrease has been noted in the frequency of transfer into a. tumefaciens st ... | 1988 | 3288538 |
| cyclic glucans produced by agrobacterium tumefaciens are substituted with sn-1-phosphoglycerol residues. | in a previous study (miller, k.j., kennedy, e.p. and reinhold, v.n. (1986) science 231, 48-51) it was reported that the biosynthesis of periplasmic cyclic beta-1,2-glucans by agrobacterium tumefaciens is strictly osmoregulated in a pattern closely similar to that found for the membrane-derived oligosaccharides of escherichia coli (kennedy, e.p. (1982) proc. natl. acad. sci. usa 79, 1092-1095). in addition to the well-characterized neutral cyclic glucan, the periplasmic glucans were found to cont ... | 1987 | 3297148 |
| a spontaneous insertion in the agrocin sensitivity region of the ti-plasmid of agrobacterium tumefaciens c58. | a spontaneous agrocin-resistant mutant of agrobacterium tumefaciens strain c58 was shown to have an insertion of 1.2 kb in the agrocin-sensitivity region of ptic58. the insertion was cloned from the ti-plasmid, and a subclone containing only dna internal to the insertion was used to probe the ti-plasmid and chromosomal dna of the wild-type strain c58. the probe showed homology to chromosomal sequences but showed no homology to wild-type ptic58. homology was also detected with chromosomal sequenc ... | 1986 | 3027729 |
| expression of nodule-specific genes in alfalfa root nodules blocked at an early stage of development. | to help dissect the molecular basis of the rhizobium-legume symbiosis, we used in vitro translation and northern blot analysis of nodule rna to examine alfalfa-specific genes (nodulins) expressed in two types of developmentally defective root nodules elicited by rhizobium meliloti. fix- nodules were elicited by r. meliloti nif mutants; these nodules were invaded by rhizobia and contained differentiated bacteroids. 'empty' nodules were elicited by r. meliloti exo and ndv mutants and by agrobacter ... | 1988 | 3417147 |
| vir box sequences in agrobacterium tumefaciens ptic58 and a6. | | 1988 | 3419938 |
| complementation of a threonine dehydratase-deficient nicotiana plumbaginifolia mutant after agrobacterium tumefaciens-mediated transfer of the saccharomyces cerevisiae ilv1 gene. | the saccharomyces cerevisiae ilv1 gene, encoding threonine dehydratase (ec 4.2.1.16) was fused to the transferred dna nopaline synthase promoter and the 3' noncoding region of the octopine synthase gene. it was introduced, by agrobacterium tumefaciens-mediated gene transfer, into an isoleucine-requiring nicotiana plumbaginifolia auxotroph deficient in threonine dehydratase. functional complementation by the ilv1 gene product was demonstrated by the selection of several transformed lines on a med ... | 1987 | 3302681 |
| agrobacterium-mediated infectivity of cloned digitaria streak virus dna. | a monomeric clone of double-stranded dna synthesized in vitro dna of the geminivirus digitaria streak (dsv) was subcloned as a tandem dimeric unit into a binary vector of agrobacterium tumefaciens, creating a plasmid pds2. inoculation of digitaria sanguinalis with a. tumefaciens carrying pds2 resulted in viral infection. the symptoms, virus particles, and dna forms obtained were indistinguishable from those of a natural dsv infection of d. sanguinalis. inoculations have also induced infections i ... | 1988 | 3341112 |
| relative strengths of the 35s cauliflower mosaic virus, 1', 2', and nopaline synthase promoters in transformed tobacco sugarbeet and oilseed rape callus tissue. | the 35s promoter of cauliflower mosaic virus and promoters from the nopaline synthase, 1' and 2' genes of agrobacterium tumefaciens t-dna were fused to the bacterial octopine synthase and chitinase gene coding regions. these chimaeric gene constructions were introduced into tobacco, sugarbeet and oilseed rape cells and their relative levels of expression measured by primer extension analysis of rna isolated from pooled populations of stably transformed calli. in tobacco callus, the 35s promoter ... | 1988 | 3163765 |
| a case of septicaemia caused by agrobacterium radiobacter. | | 1988 | 3351321 |
| expression of alfalfa mosaic virus cdna1 and 2 in transgenic tobacco plants. | chimeric genes composed of dna complementary to alfalfa mosaic virus (aimv) rnas 1 or 2, the camv 35 s promoter, and the nos polyadenylation signal were transferred to the genome of nicotiana tabacum cv. samsun nn by means of the agrobacterium tumefaciens transformation system. transformants contained intact copies of the viral genes and accumulated transcripts of approximately the size predicted from the cloning procedure. using antisera raised against synthetic peptides corresponding to the c- ... | 1988 | 3354206 |
| structure of the octopine synthase upstream activator sequence. | we have identified a transcriptional activating element within the 5' flanking sequence of the agrobacterium tumefaciens octopine synthase (ocs) gene that is necessary for ocs expression in transformed tobacco calli. this element is located between 333 and 116 base pairs upstream from the transcription initiation site and functions independent of orientation when placed upstream of the ocs gene. it does not function in either orientation when placed downstream of the gene, nor can it activate it ... | 1988 | 3357881 |
| detection of opines by colorimetric assay. | a colorimetric procedure for confirming the presence of arginine-derived opines (nopaline and octopine) in plant tissue extracts is described. those materials are widely used as markers of plant cell transformation and tumorigenesis mediated by the tumor-inducing plasmids of agrobacterium tumefaciens. nopaline and octopine are generally detected, following resolution by paper electrophoresis, by observation of the uv-fluorescent products formed upon reaction with phenanthrenequinone. we found th ... | 1987 | 3578760 |
| molecular characterization of the virc genes of the ti plasmid. | the virc (formerly bak) complementation group of the nopaline-type ti plasmid ptic58 encodes two proteins, virc1 and virc2. according to the primary structure of the polypeptides predicted by the nucleotide sequence, virc1 is composed of 231 amino acids with a total molecular mass of 25.5 kilodaltons, and virc2 is composed of 202 amino acids with a molecular mass of 22.1 kilodaltons. the ptic58 virc1 and virc2 polypeptides are equal in length to virc1 and virc2 of the octopine-type plasmid ptia6 ... | 1987 | 3584058 |
| scanning electron microscope studies of agrobacterium tumefaciens attachment to zea mays, gladiolus sp., and triticum aestivum. | scanning electron microscope studies demonstrated that cells of agrobacterium tumefaciens strains attach to cells on the cut surfaces of corn and wheat seedlings and to gladiolus disks. bacterial cells attached to these monocots in the same manner as they attached to the dicots tested. of the strains tested, a66 and t37 covered more of the cut surfaces of these monocots in a nonrandom fashion than did cells of other isolates. these bacteria attached to cells of intact monocotyledonous plants and ... | 1988 | 3360748 |
| studies on the pharmacological activity of amazonian euphorbiaceae. | plant material from 34 amazonian species of the family euphorbiaceae were collected and extracts prepared. sixteen of these species have a documented use as a medicinal agent. the extracts were tested for their ability to inhibit the growth of the bacteria, escherichia coli and staphylococcus aureus; the yeasts, saccharomyces cerevisiae and candida albicans; the dermatophytic fungi, microsporum canis, microsporum fulvum, microsporum gypseum and trichophyton gallinae; the viruses, sindbis virus a ... | 1988 | 3374150 |
| role of iron in the enhancement by agrobacterium tumefaciens infection in mice. | microorganisms require iron for their growth and usually compete with their host for available iron from the system. iron supplementation to host causes an increase of available iron both to host and to potential microbial invaders and favours the latter more than the former as the bacteria release siderophores which are responsible for iron transport mechanism. in view of this observation a study was done to deal with the distribution of storage and injected iron given as an overload within a p ... | 1988 | 3181831 |
| activation of agrobacterium tumefaciens vir gene expression generates multiple single-stranded t-strand molecules from the ptia6 t-region: requirement for 5' vird gene products. | agrobacterium tumefaciens transfers its ti-plasmid t-dna to plant cells. this process is initiated by plant-induced activation of the ti-plasmid virulence loci, resulting in the generation of single stranded (ss) cleavages of the ti-plasmid t-dna border sequences (border nicks) and ss linear unipolar t-dna molecules (t-strands). a single t-strand is produced from the two-border t-region of the pgv3850 nopaline plasmid. in this paper the induced molecular events for the complex t-region of the pt ... | 1987 | 3595560 |
| studies on the toxicity of agrobacterium tumefaciens in mice. | | 1988 | 3235104 |
| binding-protein-dependent glucose transport by agrobacterium radiobacter grown in glucose-limited continuous culture. | agrobacterium radiobacter ncib 11883 was grown in glucose-limited continuous culture at low dilution rate. whole cells transported glucose using an energy-dependent mechanism which exhibited an accumulation ratio greater than 2000. three major periplasmic proteins were purified and their potential role as glucose-binding proteins (gbp) were investigated using equilibrium dialysis. two of these, gbp1 (mr 36,500) and gbp2 (mr 33,500), bound d-glucose with high affinity (kd 0.23 and 0.07 microm res ... | 1988 | 3269386 |
| reiterated dna sequences in rhizobium and agrobacterium spp. | repeated dna sequences are a general characteristic of eucaryotic genomes. although several examples of dna reiteration have been found in procaryotic organisms, only in the case of the archaebacteria halobacterium halobium and halobacterium volcanii [c. sapienza and w. f. doolittle, nature (london) 295:384-389, 1982], has dna reiteration been reported as a common genomic feature. the genomes of two rhizobium phaseoli strains, one rhizobium meliloti strain, and one agrobacterium tumefaciens stra ... | 1987 | 3450286 |
| delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. | a chimeric gene containing a cloned cdna of the coat protein (cp) gene of tobacco mosaic virus (tmv) was introduced into tobacco cells on a ti plasmid of agrobacterium tumefaciens from which tumor inducing genes had been removed. plants regenerated from transformed cells expressed tmv mrna and cp as a nuclear trait. seedlings from self-fertilized transgenic plants were inoculated with tmv and observed for development of disease symptoms. the seedlings that expressed the cp gene were delayed in s ... | 1986 | 3457472 |
| analysis of agrobacterium tumefaciens virulence mutants in leaf discs. | the leaf disc transformation system provides a simple means to score expression of various t-dna markers within days of infection by agrobacterium tumefaciens as well as long-term selection for growth of transformed callus and shoots. in this report, we describe the application of this system to evaluation of marker transfer and integration in a comprehensive set of defined avirulent mutants of a. tumefaciens. we conclude that virc is not essential when the t-dna is present on a binary vector. t ... | 1986 | 3458219 |
| the right border region of ptit37 t-dna is intrinsically more active than the left border region in promoting t-dna transformation. | deletions of border regions of t-dna on agrobacterium ti plasmid or mini-t plasmid have shown the right border region of ptit37 t-dna to be more active than the left border region in promoting t-dna transformation. in this study we examine the possibility that the apparent difference in activity of left and right border regions may be due to position or orientation differences between the left and right borders with respect to the transferred onc genes. we have constructed eight similar single-b ... | 1986 | 3459162 |
| expression of mouse dihydrofolate reductase gene confers methotrexate resistance in transgenic petunia plants. | transgenic petunia plants containing an altered (leu22----arg22) mouse dihydrofolate reductase gene fused to the cauliflower mosiac virus 35s (camv 35s) promoter and nopaline synthase (nos) polyadenylation site were obtained by transforming petunia leaf disks with an agrobacterium tumefaciens strain carrying the chimeric gene. transformants were directly selected for and rooted on medium containing 1 microm methotrexate (mtx). the chimeric gene was present in the regenerated plants at one to thr ... | 1987 | 3468634 |
| the promoter proximal region in the vird locus of agrobacterium tumefaciens is necessary for the plant-inducible circularization of t-dna. | the formation of crown gall tumours involves the transfer of the t-dna region of the ti plasmid from agrobacterium to plant cells and its subsequent integration into plant chromosomes. when agrobacteria are incubated with plant protoplasts or exudates of plants, the t-dna region is circularized by recombination or cleavage and rejoining between the 25 bp terminal repeats; the formation of circular t-dnas is thought to be one step in t-dna transfer (koukolikova-nicola et al. 1985; machida et al. ... | 1987 | 3472033 |
| mobilization of t-dna from agrobacterium to plant cells involves a protein that binds single-stranded dna. | crude protein extracts of induced and uninduced octopine wild-type strain of agrobacterium tumefaciens, as well as several mutants of the virulence loci vira, -b, -g, -c, -d, and -e, were probed with single- and double-stranded synthetic oligodeoxynucleotides of different sequence and length in an electrophoretic retardation assay. four complexes involving sequence-nonspecific, single-stranded-dna-binding proteins were recognized. one inducible complex is determined by the vire locus, two ti-pla ... | 1987 | 3480525 |
| molecular basis for the auxin-independent phenotype of crown gall tumor tissues. | the transfer of specific ti (tumor-inducing) plasmid sequences, the t-dna, from agrobacterium tumefaciens to a wide range of plants results in the formation of crown gall tumors. these tissues differ from most plant cells in that they can be grown in vitro in the absence of added phytohormones. here, data are presented that offer an explanation for the auxin-independent phenotype of crown gall tissues. it is shown that crude cell-free extracts prepared from three bacterial species harboring ptia ... | 1986 | 3511528 |
| promoters of agrobacterium tumefaciens ti-plasmid virulence genes. | the dna sequences of the promoter and 5' upstream regions of six agrobacterium tumefaciens ti-plasmid encoded virulence (vir) genes were determined. the transcription initiation sites were mapped by the s1 nuclease protection assay. in the -10 region, the vir promoters share a consensus sequence that is homologous to a dna sequence found in the same region of e. coli promoters. in contrast, the -35 region sequences are variable. several vir genes contain two common hexanucleotide sequences, 5'cg ... | 1986 | 3513123 |
| nucleotide sequence and expression of a pseudomonas savastanoi cytokinin biosynthetic gene: homology with agrobacterium tumefaciens tmr and tzs loci. | the nucleotide sequence of a pseudomonas trans-zeatin producing gene (ptz) from the pck1 plasmid of pseudomonas syringae pv. savastanoi strain 1006 has been determined. this gene confers upon e. coli the ability to synthesize and secrete several cytokinins including trans-zeatin, iso-pentenyladenine and their respective n9-ribosyl derivatives. sequence analysis indicates an open reading frame encoding a protein of 234 amino acids with a molecular weight of 26,816. significant sequence homology i ... | 1986 | 3515320 |
| fate of selectable marker dna integrated into the genome of nicotiana tabacum. | to compare the effects of different transformation methods on the integration behavior and structural stability of integrated foreign genes in plant cells, tobacco protoplasts were transformed with escherichia coli plasmid plgv2103neo dna using the ca phosphate dna coprecipitation technique. parallel transformations were done by cocultivation with agrobacterium tumefaciens harboring the ti plasmid derivatives pgv3850::2103neo or pgv3850::1103neo. a comparison of the fine structure of the integra ... | 1986 | 3519133 |
| modification of t-dna of nopaline ti plasmid by intermediary vector and utilization of agrocin 84 sensitivity as simple criterion of conjugation transfer of modified ti plasmid. | the chloramphenicol resistance gene from psa was introduced into t-dna of pti t37 of agrobacterium tumefaciens by cointegration with intermediary plasmid based on pbr322. the resulting intermediary vector was mobilized to a. tumefaciens t37 by conjugative plasmid prk2. the rk2 plasmid also forms contegrates with pti due to the tn3 transposon which was used for the mobilization of modified pti into plasmid-less a. tumefaciens strain. transconjugants were selected on the basis of their antibiotic ... | 1986 | 3519390 |
| identification of legionella pneumophila with commercially available immunofluorescence test. | | 1986 | 3522642 |
| initial interactions of agrobacterium tumefaciens with plant host cells. | infections of wounded dicotyledonous plants by agrobacterium tumefaciens result in the formation of crown gall tumors. the initial step in tumor formation is the site-specific attachment of the bacteria to the host cells. the mechanism of recognition and attachment in this interaction has been studied in detail. current information on the nature of the bacterial binding sites, the nature of the host receptors, the role of bacterial cellulose fibrils, and the genetics of bacterial attachment will ... | 1986 | 3533427 |
| characterization of the vire operon of the agrobacterium ti plasmid ptia6. | the agrobacterium tumefaciens ti plasmid contains at least six transcriptional units (designated vir loci) which are essential for efficient crown gall tumorigenesis. mutations in one of these loci, vire, result in a sharply attenuated virulence phenotype. in the present communication, we have analyzed the vire operon at the molecular level. this locus contains open reading frames coding for two hydrophilic proteins having molecular weights of approximately 7,000 daltons and 60,500 daltons. usin ... | 1987 | 3547330 |
| proline biosynthesis encoded by the noc and occ loci of agrobacterium ti plasmids. | octopine or nopaline ti plasmids, or clones encoding their occ or noc loci, allowed proline auxotrophs of agrobacterium tumefaciens to utilize the appropriate arginyl opine as a proline substitute. arginine and ornithine substituted for proline only if the occ or noc loci were induced or made constitutive by mutation. these results support a report demonstrating a ti plasmid-encoded activity in a. tumefaciens which cyclizes ornithine to proline. | 1986 | 3733675 |
| characterization of the vire locus of agrobacterium tumefaciens plasmid ptic58. | the vire locus that is responsible for the efficiency of infection by agrobacterium tumefaciens (t. hirooka and c. kado, j. bacteriol. 168:237-243, 1986) is located next to the right boundary of the virulence (vir) region of the nopaline plasmid ptic58. this locus is very similar to the vire locus of octopine type ti plasmids on the basis of nucleotide and amino acid sequence comparisons as well as genetic complementation analyses. the nucleotide sequence of vire revealed three open reading fram ... | 1987 | 3549694 |
| agrobacterium tumefaciens-mediated transformation of the monocot genus gladiolus: detection of expression of t-dna-encoded genes. | agrobacterium tumefaciens was capable of directing the transformation of gladiolus sp., a monocot genus belonging to the family iridaceae. only strains capable of transferring t-dna formed tumors, sections of which could be cultured in phytohormone-free media. opine synthase activities were also observed in homogenates made from these tumors. | 1987 | 3558323 |
| molecular characterization of a host-range-determining locus from agrobacterium tumefaciens. | the virulence loci play an essential role in tumor formation by agrobacterium tumefaciens. this study focused on the virc locus, which affects the host range agrobacterium species. virc mutants display an attenuated or avirulent phenotype on certain host plants, but remain fully virulent on other plant hosts. the nucleotide sequence revealed that the virc locus of ptia6nc is an operon consisting of two open reading frames. these two open reading frames, designated virc1 and virc2, encode protein ... | 1986 | 3759904 |
| common loci for agrobacterium tumefaciens and rhizobium meliloti exopolysaccharide synthesis and their roles in plant interactions. | mutants of rhizobium meliloti have been isolated which are deficient in exopolysaccharide (eps) production and effective nodulation of alfalfa (j. a. leigh, e. r. signer, and g. c. walker, proc. natl. acad. sci. usa 82:6231-6235, 1985). we isolated approximately 100 analogous eps-deficient (exo) mutants of the closely related plant pathogen agrobacterium tumefaciens, including strains whose eps deficiencies were specifically complemented by each of five cloned r. meliloti exo loci. we also clone ... | 1987 | 3571162 |
| nitrogen-fixing nodules induced by agrobacterium tumefaciens harboring rhizobium phaseoli plasmids. | rhizobium phaseoli cfn299 forms nitrogen-fixing nodules in phaseolus vulgaris (bean) and in leucaena esculenta. it has three plasmids of 185, 225, and 410 kilobases. the 410-kilobase plasmid contains the nitrogenase structural genes. we have transferred these plasmids to the plasmid-free strain agrobacterium tumefaciens gmi9023. transconjugants containing different combinations of the r. phaseoli plasmids were obtained, and they were exhaustively purified before nodulation was assayed. only tran ... | 1987 | 3584072 |
| agrobacterium tumefaciens ptar para promoter region involved in autoregulation, incompatibility and plasmid partitioning. | the locus responsible for directing proper plasmid partitioning of agrobacterium tumefaciens ptar is contained within a 1259 base-pair region. insertions or deletions within this locus can result in the loss of the plasmid's ability to partition properly. one protein product (para), approximately 25,000 mr, is expressed from the par locus in escherichia coli and a. tumefaciens protein analysis systems in vitro. dna sequence analysis of the locus revealed a single 23,500 mr open reading frame, co ... | 1987 | 3586028 |
| characterization of the vira locus of agrobacterium tumefaciens: a transcriptional regulator and host range determinant. | the virulence (vir) region of agrobacterium tumefaciens mediates the transfer of a defined segment of plasmid dna (the t-dna) into the plant genome. the vir genes are specifically induced by molecules produced by wounded plant cells, and vira is required for this induction. we have determined the nucleotide sequence of vira loci from limited (ptiag162) and wide (ptia6) host range tumor-inducing (ti) plasmids, each of which encodes a single protein of 92,000 daltons. using antibody directed again ... | 1987 | 3595559 |
| expression of rhizobium meliloti nod genes in rhizobium and agrobacterium backgrounds. | rhizobium meliloti nod genes are required for the infection of alfalfa. induction of the nodc gene depends on a chemical signal from alfalfa and on nodd gene expression. by using a nodc-lacz fusion, we have shown that the induction of the r. meliloti nodc gene and the expression of nodd occur at almost normal levels in other rhizobium backgrounds and in agrobacterium tumefaciens, but not in escherichia coli. xanthomonas campestris, or pseudomonas savastanoi. our results suggest that bacterial ge ... | 1987 | 3597319 |
| nucleotide sequence of the virg locus of the agrobacterium tumefaciens plasmid ptic58. | the nucleotide sequence of the virg locus of the nopaline type plasmid ptic58 of agrobacterium tumefaciens has been determined. it contains an open reading frame (orf) of 759 nucleotides and has 77% homology to the virg sequences of octopine type plasmids. differences between the sequences of the two types of ti plasmids in the region of virg are located predominantly outside the orf. the amino acid sequences inferred from the two virg genes show 80% homology to each other and each shows the sam ... | 1987 | 3448462 |
| structure of a polysaccharide containing galactose and galacturonic acid from rhizobium meliloti. characterization and partial purification of a 2-o-methyltransferase. | the teichuronic acid type polysaccharide found in rhizobium meliloti which is associated with sensitivity to phage 16b and is formed in the inner membranes from udp-galactose and udp-galacturonic acid (ugalde, r. a., coira, j. a., and brill, w. j. (1986) j. bacteriol. 168, 270-275) has been studied further. results of acid hydrolysis, periodate oxidation, and borohydride reduction show that this polysaccharide contains the repetitive unit -galacturonosyl(1-3)galactosyl(1-4-). a soluble enzyme wa ... | 1987 | 3611083 |
| pseudomonas stutzeri septicemia. | | 1987 | 3611326 |
| cytokinin production by agrobacterium and pseudomonas spp. | the production of cytokinins by plant-associated bacteria was examined by radioimmunoassay. strains producing trans-zeatin were identified in the genera agrobacterium and pseudomonas. agrobacterium tumefaciens strains containing nopaline tumor-inducing plasmids, a. tumefaciens lippia isolates, and agrobacterium rhizogenes strains produced trans-zeatin in culture at 0.5 to 44 micrograms/liter. pseudomonas solanacearum and pseudomonas syringae pv. savastanoi produced trans-zeatin at levels of up t ... | 1987 | 3624204 |
| molecular characterization of the vird operon from agrobacterium tumefaciens. | the agrobacterium tumefaciens ti plasmid virulence (vir) region contains at least six transcriptional units required for the efficient transfer of t-dna to the plant genome (vira, b, c, d, e, and g). we have reported that two proteins encoded by the 5'portion of the vird operon are required for a site-specific endonuclease activity that nicks the direct repeats which flank the t-dna. we have presented the nucleotide sequence for this portion of the operon. the nucleotide sequence of the remainde ... | 1987 | 3658701 |
| the molecular basis of plant cell transformation by agrobacterium tumefaciens. | | 1985 | 3893414 |
| dual control of agrobacterium tumefaciens ti plasmid virulence genes. | the virulence genes of nopaline (ptic58) and octopine (ptia6nc) ti plasmids are similarly affected by the agrobacterium tumefaciens ros mutation. of six vir region complementation groups (vira, virb, virg, virc, vird, and vire) examined by using fusions to reporter genes, the promoters of only two (virc and vird) responded to the ros mutation. for each promoter that was affected by ros, the level of expression of its associated genes was substantially elevated in the mutant. this increase was no ... | 1987 | 3667525 |
| chemotaxis to plant phenolic inducers of virulence genes is constitutively expressed in the absence of the ti plasmid in agrobacterium tumefaciens. | the virulence (vir) genes are required in the early stages of plant tumor formation and are located together on the tumor-inducing (ti) plasmid in agrobacterium tumefaciens. five of the vir genes are expressed inducibly in response to the following monocyclic phenolic compounds: acetosyringone, catechol, gallate, beta-resorcylate, protocatechuate, p-hydroxybenzoate, and vanillin. of these compounds, only the latter six, excluding vanillin [corrected] served as chemoattractants and only the latt ... | 1987 | 3667536 |
| plant expression signals of the agrobacterium t-cyt gene. | within the 5' and 3' non-coding regions of the t-cyt gene from the octopine t-dna of agrobacterium tumefaciens sequences required for expression of this gene in plant cells were identified by deletion mutagenesis. the results show that 184 bp of the 5' non-coding region and 270 bp of the 3' non-coding region are sufficient for wild-type expression. within the 5' non-coding region two essential expression signals were identified: (1.) an activator element located between -185 and -129 with respec ... | 1987 | 3671083 |
| effects of mutations in the tata box region of the agrobacterium t-cyt gene on its transcription in plant tissues. | we have generated mutations in the promoter region of the octopine type cytokinin gene of agrobacterium tumefaciens, and studied their effects on mrna formation in different plant species. the promoter region of this gene contains several putative tata boxes. phenotypic expression and northern blot hybridization showed that tata boxes are essential for expression, but that one tata box leads to wild-type transcript levels. analysis of the 5' ends of t-cyt transcripts by primer extension using rn ... | 1987 | 3671084 |
| agrobacterium tumefaciens virulence locus psca is related to the rhizobium meliloti exoc locus. | agrobacterium tumefaciens and rhizobium meliloti carry related genetic loci which have important roles in virulence and symbiosis. previously, it was shown that two virulence loci of a. tumefaciens, chva and chvb, are related to two r. meliloti symbiosis loci, ndva and ndvb, respectively (t. dylan, l. ielpi, s. stanfield, l. kashyap, c. douglas, m. yanofsky, e. nester, d. r. helinski, and g. ditta, proc. natl. acad. sci. usa 83:4403-4407, 1986). here we show that these two phytobacteria possess ... | 1987 | 3680180 |
| overdrive is a t-region transfer enhancer which stimulates t-strand production in agrobacterium tumefaciens. | introduction of a left or right synthetic border repeat together with the overdrive sequence in an octopine ti-plasmid deletion mutant, lacking the right border, resulted in the complete restoration of the oncogenicity of the mutant strain. however introduction of a border repeat without the overdrive, only restored oncogenicity partially. the overdrive sequence turned out to be able to stimulate the synthetic border mediated t-region transfer, independent of its orientation and position relativ ... | 1987 | 3684577 |
| enhanced instrumental sensitivity and selectivity for aminopeptidase profiling. | laser-excited fluorimetry has been applied to the identification of bacteria and fungus. the instrumental sensitivity and selectivity of the aminopeptidase profiling method has been enhanced by the use of laser excitation in conjunction with improved spectral and temporal background rejection. the linear dynamic range for the aminopeptidase technique has been increased by achieving a reduced lower limit of detection of the fluorescent tag, beta-naphthylamine. standard aminopeptidase methodology ... | 1986 | 3706731 |
| nodules are induced on alfalfa roots by agrobacterium tumefaciens and rhizobium trifolii containing small segments of the rhizobium meliloti nodulation region. | regions of the rhizobium meliloti nodulation genes from the symbiotic plasmid were transferred to agrobacterium tumefaciens and rhizobium trifolii by conjugation. the a. tumefaciens and r. trifolii transconjugants were unable to elicit curling of alfalfa root hairs, but were able to induce nodule development at a low frequency. these were judged to be genuine nodules on the basis of cytological and developmental criteria. like genuine alfalfa nodules, the nodules were initiated from divisions of ... | 1985 | 3968028 |
| tomato golden mosaic virus a component dna replicates autonomously in transgenic plants. | phenotypically normal petunia plants carrying chromosomal inserts of either the tomato golden mosaic virus (tgmv) a or the b component dna, as single or tandem inserts, were obtained using an agrobacterium tumefaciens ti plasmid-based transformation system. southern hybridization analysis revealed that the tandem, direct-repeat a plants contained free single and double stranded a component dnas. no free b component dna was detected in plants carrying tandem repeats of the b component. progeny of ... | 1986 | 3708687 |
| transformed cell clones as a tool to study t-dna integration mediated by agrobacterium tumefaciens. | a large number of tobacco sr1 cell clones transformed by the wild-type agrobacterium c58 have been analysed for the presence of screenable markers such as tumour morphology, opine synthesis and hormone dependence. distinct phenotypic classes were observed depending upon whether the cell clones were isolated from primary tumours or were obtained via cocultivation of protoplasts. these classes of tobacco sr1-c58 transformants appear to arise from errors in the ti plasmid (t-dna) transfer and integ ... | 1986 | 3723593 |
| an intermediate in cyclic beta 1-2 glucan biosynthesis. | incubation of udp-[14c]glc with the inner membranes of agrobacterium tumefaciens leads to the formation of cyclic beta 1-2 glucan and trichloroacetic acid-insoluble compounds. the proteolysis products of the latter show a positive charge in acid and a negative charge in alkaline buffers. the cyclic beta 1-2 glucan and the trichloroacetic acid insoluble compounds yield the same products on partial acid hydrolysis. addition of excess non-radioactive udp-glc to the reaction mixture nearly stops the ... | 1985 | 3970697 |
| vira and virg control the plant-induced activation of the t-dna transfer process of a. tumefaciens. | the ti plasmid vir loci of agrobacterium tumefaciens are transcriptionally activated in response to signal molecules produced by plant cells to initiate the t-dna transfer process. we show that the ptia6 vir loci are organized as a single regulon whose induction by plants is controlled by vira and virg. mutations in vira result in attenuated induction. this locus is constitutively transcribed and noninducible. mutations in virg eliminate vir induction. this locus is constitutively transcribed, p ... | 1986 | 3731272 |
| two gene clusters of rhizobium meliloti code for early essential nodulation functions and a third influences nodulation efficiency. | a plafr1 cosmid clone (ppp346) carrying the nodulation region of the symbiotic plasmid prme41b was isolated from a gene library of rhizobium meliloti 41 by direct complementation of a nod- deletion mutant of r. meliloti. agrobacterium tumefaciens and rhizobium species containing ppp346 were able to form ineffective nodules on alfalfa. the 24-kilobase insert in ppp346 carries both the common nodulation genes and genes involved in host specificity of nodulation. it was shown that these two regions ... | 1986 | 3745124 |
| control of a futile urea cycle by arginine feedback inhibition of ornithine carbamoyltransferase in agrobacterium tumefaciens and rhizobia. | in agrobacterium tumefaciens and rhizobia arginine can be used as the sole nitrogenous nutrient via degradation by an inducible arginase. these microorganisms were found to exhibit arginine inhibition of ornithine carbamoyltransferase activity. this inhibition is competitive with respect to ornithine (km for ornithine = 0.8 mm; ki for arginine = 0.05 mm). this type of urea cycle regulation has not been observed among other microorganisms which degrade arginine via an arginase. the competitive pa ... | 1986 | 3758074 |
| time required for tumor induction by agrobacterium tumefaciens. | cellulose-minus mutants of agrobacterium tumefaciens retain virulence but can be removed from wound sites by washing with water. washing of bryophyllum diagremontiana leaves inoculated with a cellulose-minus mutant was used to determine the minimum time the bacteria must be present for tumor induction. this time was 4 to 8 h. | 1986 | 3767365 |
| agrobacterium tumefaciens and the susceptible plant cell: a novel adaptation of extracellular recognition and dna conjugation. | | 1986 | 3768954 |
| molecular cloning and expression of rhizobium fredii usda 193 nodulation genes: extension of host range for nodulation. | dna hybridization with the cloned nodulation region of rhizobium meliloti as a probe revealed dna homology with four hindiii fragments, 12.5, 6.8, 5.2, and 0.3 kilobases (kb) in size, of the symbiotic plasmid prjausda193. both hybridization and complementation studies suggest that the common nodulation genes nodabc and nodd of r. fredii usda 193 are present on the 5.2-kb hindiii and 2.8-kb ecori fragments, respectively, of the sym plasmid. both fragments together could confer nodulation ability ... | 1986 | 3782034 |
| the hypervirulence of agrobacterium tumefaciens a281 is encoded in a region of ptibo542 outside of t-dna. | we used a binary-vector strategy to study the hypervirulence of agrobacterium tumefaciens a281, an l,l-succinamopine strain. strain a281 is hypervirulent on several solanaceous plants. we constructed plasmids (pcs65 and pcs277) carrying either the transferred dna (t-dna) or the remainder of the tumor-inducing (ti) plasmid (peha101) from this strain and tested each of these constructs in trans with complementary regions from heterologous ti plasmids. hypervirulence on tobacco could be reconstruct ... | 1986 | 3782037 |
| role for 2-linked-beta-d-glucan in the virulence of agrobacterium tumefaciens. | phenol-water cell extracts of virulent agrobacterium tumefaciens a348 and several avirulent mutants with a reduced ability to attach to plant surfaces were examined. a low-molecular-weight 2-linked-beta-d-glucan was identified in the cell wall extracts of the virulent wild-type strain. analyses of phenol-water extracts and culture filtrates of four mutant strains showed that the mutants did not produce any 2-linked-beta-d-glucan. when these mutants were complemented, the ability to produce the g ... | 1985 | 4044517 |
| bacterial carbon-phosphorus lyase: products, rates, and regulation of phosphonic and phosphinic acid metabolism. | carbon-phosphorus bond cleavage activity, found in bacteria that utilize alkyl- and phenylphosphonic acids, has not yet been obtained in a cell-free system. given this constraint, a systematic examination of in vivo c-p lyase activity has been conducted to develop insight into the c-p cleavage reaction. six bacterial strains were obtained by enrichment culture, identified, and characterized with respect to their phosphonic acid substrate specificity. one isolate, agrobacterium radiobacter, was s ... | 1987 | 3804975 |
| a rhizobium meliloti mutant that forms ineffective pseudonodules in alfalfa produces exopolysaccharide but fails to form beta-(1----2) glucan. | a mutant of rhizobium meliloti that elicited the formation of inactive nodules in alfalfa was found not to form beta-(1----2) glucan in vivo or in vitro. it was nonmotile because it lacks flagella. the 235-kilodalton protein which acts as an intermediate in beta-(1----2) glucan synthesis was undetectable in the mutant. these properties of the mutant are common to those of chvb mutants of agrobacterium tumefaciens. exopolysaccharide formation by the r. meliloti mutant was about double that by the ... | 1987 | 3804979 |
| identification of a new virulence locus in agrobacterium tumefaciens that affects polysaccharide composition and plant cell attachment. | we have identified a new virulence locus in agrobacterium tumefaciens. strains carrying tn5 inserts at this locus could not incite tumors on kalanchoe daigremontiana, nicotiana rustica, tobacco, or sunflower and had severely attenuated virulence on carrot disks. we termed the locus psca, because the mutants that defined the locus were initially isolated as having an altered polysaccharide composition; they were nonfluorescent on media containing leucophor or calcofluor, indicating a defect in th ... | 1987 | 3597321 |
| characterization of agrobacterium tumefaciens strains isolated from grapevine tumors in china. | thirteen strains of agrobacterium tumefaciens isolated from grapevine tumors in northern china were surveyed. these strains varied in their host range properties, although all were tumorigenic on grapevines. twelve of these strains belonged to agrobacterium sp. biotype 3, and 11 strains resulted in the synthesis of the opine octopine in tumor tissue. interestingly, one strain resulted in accumulation of arginine, a previously unrecognized opine, in tumor tissue. although dna in most of these str ... | 1987 | 3606110 |
| selection-expression plasmid vectors for use in genetic transformation of higher plants. | plasmid vectors containing both a selectable marker for plant transformation (kanamycin resistance) and a second, directly adjacent, divergent promoter for the transcription of inserted dna fragments have been constructed. these vectors make use of a small (479 bp) dual-promoter dna fragment, originally isolated from the t-dna of agrobacterium tumefaciens, fused to the neomycin phosphotransferase gene of tn5. several unique restriction enzyme cleavage sites, as well as a polyadenylation signal s ... | 1985 | 4059052 |
| [effect of agrobacterium tumefaciens (smith and town). conn. on the functional properties of human blood lymphocytes]. | agrobacterium tumefaciens has been shown to affect the proliferation of intact lymphocytes as well as lymphocytes stimulated by pha action. inoculation of bacteria into the cultures of intact lymphocytes and incubation during 72 h resulted in increased incorporation of [3h]-thymidine into the dna of cultivated cells. the bacteria are capable of inhibition of lymphocytes proliferation stimulated by pha. | 1985 | 3842755 |
| surface components involved in bacterial pathogen-plant host recognition. | during their initial association with plant hosts, pathogenic bacteria interact with plant cell walls. the results of this interaction appear to determine whether bacterial multiplication will take place. with one group of bacterial plant pathogens (e.g. agrobacterium tumefaciens), attachment to the host surface appears essential for pathogenesis. with another group (e.g. pseudomonas solanacearum), only those strains that do not attach to the host cell wall are able to multiply in the intercellu ... | 1985 | 3867676 |
| mitochondrial origins. | the 16s ribosomal rna sequences from agrobacterium tumefaciens and pseudomonas testosteroni have been determined to further delimit the origin of the endosymbiont that gave rise to the mitochondrion. these two prokaryotes represent the alpha and beta subdivisions, respectively, of the so-called purple bacteria. the endosymbiont that gave rise to the mitochondrion belonged to the alpha subdivision, a group that also contains the rhizobacteria, the agrobacteria, and the rickettsias--all prokaryote ... | 1985 | 3892535 |
| eight dna insertion events of agrobacterium tumefaciens ti-plasmids in isogenic sunflower genomes are all distinct. | we investigated whether the same or different t-dna insertions occur every time agrobacterium tumefaciens, the octopine type strain pti 15955 strr, infects genetically identical sunflower plants. eight newly established crown gall tissue culture lines were analyzed for their t-dna content. our data showed that all isogenic crown gall callus dna produced distinct hybridization patterns. these eight patterns were also different from three standard lines included for comparison. in addition, all th ... | 1985 | 3929775 |
| the in vitro effects of opines and other compounds on dnas originating from bacteria, and from healthy and tumorous plant tissues. | purified total dnas were isolated from oncogenic or nononcogenic agrobacterium tumefaciens cells as well as from normal and crown gall tissues. opines (octopine, nopaline, lysopine), plant hormone (auxin iaa) and some carcinogenic compounds were used in order to correlate their effects on in vitro strand separation and synthesis of dnas with in vivo tumorous cell multiplication. octopine (or nopaline) induced chain opening of dnas originating from octopine (or nopaline)-metabolizing bacteria and ... | 1985 | 3935498 |
| the use of pnj5000 as an intermediate vector for the genetic manipulation of agrobacterium ti-plasmids. | the use of broad-host-range plasmids derived from rp4 as intermediate vectors for the transfer of narrow-host-range recombinant plasmids from escherichia coli to agrobacterium tumefaciens as a preliminary to marker exchange is described. recombinant plasmids having a cole1 type origin were linked to the rp4 derivative. cointegrate formation appeared to take place by reca-independent, homologous recombination within a short piece of dna derived from the beta-lactamase gene of tn1/tn3 carried by b ... | 1985 | 4093762 |
| electron microscopy of phages for agrobacterium tumefaciens. | | 1970 | 4098556 |
| morphological, biological, and physical properties of agrobacterium tumefaciens bacteriophages. | | 1972 | 4110281 |
| a soluble cytochrome c from agrobacterium tumefaciens, strain b2a. | | 1973 | 4128307 |
| "genetic' transformation of bacteria by rna and loss of oncogenic power properties of agrobacterium tumefaciens. transforming rna as template for dna synthesis. | | 1973 | 4137363 |
| 3-ketoglucose reductase of agrobacterium tumefaciens. | two kinds of 3-ketoglucose-reducing enzyme were partially purified from the sonic extract of agrobacterium tumefaciens iam 1525 grown on a sucrose-containing medium. both enzymes have a specific requirement for reduced nicotinamide adenine dinucleotide phosphate (nadph) as a hydrogen donor and catalyze the reduction of 3-ketoglucose to glucose but do not reduce 3-ketoglucosides such as 3-ketosucrose, 3-ketoglucose-1-phosphate, 3-ketotrehalose, and 3-ketocellobiose. from the requirement and subst ... | 1973 | 4144143 |