| complete amino acid sequence and identification of the platelet glycoprotein ib-binding site of jararaca gpib-bp, a snake venom protein isolated from bothrops jararaca. | jararaca gpib-bp, a snake venom protein composed of alpha and beta subunits purified from bothrops jararaca, binds to platelet glycoprotein (gp)ib and functions as a receptor blocker for von willebrand factor binding to gpib (fujimura, y., ikeda, y., miura, s., yoshida, e., shima, h., nishida, s., suzuki, m., titani, k., taniuchi, y., and kawasaki, t. (1995) thromb. haemostasis 74, 743-750). we present here the entire 142- and 123-residue amino acid sequence of the respective alpha and beta subu ... | 1996 | 8631868 |
| meningitis caused by alcaligenes (achromobacter) xylosoxidans associated with epidural catheter. | | 1995 | 8655217 |
| human colorectal carcinomas specifically accumulate mr 42,000 ubiquitin-conjugated cytokeratin 8 fragments. | recent studies have shown that various tumor cells accumulate ubiquitin (ub)-conjugated proteins, the profiles of which differ from those of normal cells. to identify the ub-conjugated proteins accumulated specifically by human carcinoma cells, a two-dimensional immunoblot analysis of 31 surgically resected human primary colorectal carcinoma tissues was performed using an anti-ub monoclonal antibody, km691. two distinct mr 42,000 and 45,000 proteins in the triton x-insoluble fractions of carcino ... | 1996 | 8665509 |
| high-resolution crystal structures of two polymorphs of cytochrome c' from the purple phototrophic bacterium rhodobacter capsulatus. | the structures of two polymorphs of cytochrome c' from rhodobacter capsulatus (rccp) strain m110 have been determined by the molecular replacement method. iron anomalous scattering data were used to confirm the molecular replacement solution. the structures were refined at 1.72 angstrom and 2.0 angstrom resolution to r-values of 15.0% and 16.3%, respectively. the rccp molecule is a dimer and each of the identical 129 residue subunits folds as a four-helical bundle with a covalently bound heme gr ... | 1996 | 8676382 |
| dephosphorylation of fetal-tau and paired helical filaments-tau by protein phosphatases 1 and 2a and calcineurin. | we have reported that many sites of tau in fetal brain (fetal-tau) as well as in paired helical filaments (phf-tau) are phosphorylated. in the present study, we used site-specific antibodies and peptide mapping to examine protein phosphatases involved in dephosphorylation of fetal-tau and phf-tau. immunoblot analysis and electrophoretic mobility showed that protein phosphatases 1 and 2a and calcineurin could dephosphorylate fetal-tau and phf-tau. phosphoserines 199, 202, 396, and 413 and phospho ... | 1995 | 8720139 |
| purification and primary structure of a myotoxic lysine-49 phospholipase a2 with low lipolytic activity from trimeresurus gramineus venom. | four acidic phospholipase a2 (pla2) isozymes named pla2-i, ii, iii and iv have previously been isolated from trimeresurus gramineus (green habu snake) venom and sequenced [oda et al. (1991) toxicon 29, 157; fukagawa et al. (1992) toxicon 30, 1131; fukagawa et al. (1993) toxicon 31, 957]. they contain aspartate-49 which is known to bind ca2+, essential for catalysis. in the present study, a basic pla2 named pla2-v containing lysine-49 was newly isolated from the same snake venom. its isoelectric ... | 1995 | 8744986 |
| [bacteremia caused by achromobacter xylosidans]. | | 1996 | 8750544 |
| s-pyridylethylation of intact polyacrylamide gels and in situ digestion of electrophoretically separated proteins: a rapid mass spectrometric method for identifying cysteine-containing peptides. | in-gel proteolytic digestion of acrylamide-gel separated proteins is a method widely used for generating peptide fragments for the purpose of identifying proteins by edman degratation, tandem mass spectrometry, and peptide-mass fingerprinting. however, it is well recognised for disulfide-bonded proteins electrophoresed under reducing conditions that if no precautions are taken to minimise disulfide bond formation during protein digestion or peptide isolation, complex peptide maps can result. her ... | 1996 | 8783016 |
| incidence of pyrexia in patients undergoing haemodialysis. | four patients on maintenance haemodialysis at the lagos university teaching developed pyrogenic reactions during treatment. blood cultures were negative but the haemodialysates were grossly contaminated, mostly with gram-negative bacilli, implicating their endotoxin as the cause of the observed pyrogenicity. the water reservoirs supplying the dialysis centre were grossly contaminated (counts 2.0 x 10(2) cfuml-1) with gram-negative bacteria such as pseudomonas aeruginosa, ps. fluorescens, alcalig ... | 1996 | 8855673 |
| bordetella trematum sp. nov., isolated from wounds and ear infections in humans, and reassessment of alcaligenes denitrificans rüger and tan 1983. | ten strains recognized on the basis of a computer-assisted numerical comparison of whole-cell protein patterns as members of a novel species belonging to the family alcaligenaceae were examined by using an integrated phenotypic and genotypic approach. this species, for which we propose the name bordetella trematum sp. nov., was more closely related to the type species of the genus bordetella (bordetella pertussis) than to the type species of the genus alcaligenes (alcaligenes faecalis) and had t ... | 1996 | 8863408 |
| systematic peptide fragmentation of polyvinylidene difluoride(pvdf)-immobilized proteins prior to microsequencing. | the sequential in situ digestion of proteins immobilized on polyvinylidene difluoride (pvdf) has been systematically designed and optimized. the method consists of immobilization of the proteins on pvdf, s-carboxymethylation, and then successive in situ digestions using specific proteases. in order to obtain high yields of the peptide fragments, from which specific amino acid residues connected to the n- or c-terminal of the resulting digestion fragments can be deduced, the cleavages are perform ... | 1996 | 8864840 |
| achromobacter xylosoxidans bacteremia: report of four cases and review of the literature. | seventy-seven cases of bacteremia due to achromobacter xylosoxidans were reviewed, and susceptibility studies were performed on 11 clinical isolates of a. xylosoxidans. nosocomial bacteremia was noted in 54 of 77 patients (70%), and 28 (36%) had infection associated with an outbreak or acquired from a discrete point source. the most common underlying illnesses were malignancies (30%) and cardiac disease (21%); immunosuppression affected 27%. the most common clinical syndromes were primary and ca ... | 1996 | 8879782 |
| identification and partial amino acid sequences of seven s-rnases associated with self-incompatibility of japanese pear, pyrus pyrifolia nakai. | s-allele-specific proteins (s-proteins) were separated and identified by two-dimensional (2d) gel electrophoresis from the style extract of 14 cultivars of japanese pear, pyrus pyrifolia nakai, which exhibits gametophytic self-incompatibility. these s-proteins were 30-32 kda basic proteins with putative pis of 9.6-10.1 and were distinct from the other proteins, which were common for all cultivars examined. each s-protein was assigned to a given s-genotype based on electrophoretic mobility and th ... | 1996 | 8889818 |
| a substitution at his-120 in the lasa protease of pseudomonas aeruginosa blocks enzymatic activity without affecting propeptide processing or extracellular secretion. | the lasa protease of pseudomonas aeruginosa can degrade elastin and is an important contributor to the pathogenesis of this organism. lasa (20 kda) is a member of the beta-lytic endopeptidase family of extracellular bacterial proteases, and it shows high-level staphylolytic activity. we sequenced the lasa gene from strain frd1 and overexpressed it in escherichia coli. the lasa gene encodes a precursor, known as pre-prolasa, of 45,582 da. amino-terminal sequence analysis allowed the identificatio ... | 1996 | 8932318 |
| [bacteremia caused by alcaligenes (achromobacter) xylosoxidans. description of 3 cases and review of the literature]. | alcaligenes (achromobacter) xylosoxidans occasionally cause infections, mainly in immunocompromised hosts. | 1996 | 8991439 |
| [collagenolytic enzymes synthesized by microorganisms]. | the review uses data obtained by the authors and available in the literature to discuss microbial collagenolytic enzymes, widely employed in scientific research, biotechnology, and medicine. collagenases differing in their structure and the specificity of their action on collagen fibrils were isolated from bacteria (including marine isolates), actinomycetes, and fungi. collagenases produced by clostridium histolyticum and achromobacter iophagus are the best studied enzymes; both are metalloenzym ... | 1996 | 8992239 |
| location of cysteine and cystine residues in s-ribonucleases associated with gametophytic self-incompatibility. | s-ribonucleases (s-rnases) that cosegregate with s-alleles in the styles of solanaceous and rosaceous plants are associated with gametophytic self-incompatibility (gsi). the amino acid sequences of many s-rnases have been derived from cdna sequences, but the state of half-cystines has not been clarified. we report the locations of the two free cysteine residues and four disulfide bridges of tobacco s6-rnase and of the four disulfide bridge of japanese pear s4-rnase. the protein was first s-pyrid ... | 1996 | 9022690 |
| characterization and distribution of o-glycosylated carbohydrates in the cell adhesion molecule, contact site a, from dictyostelium discoideum. | this paper presents further investigation of the properties of carbohydrate ii in the cell adhesion molecule, contact site a, from dictyostelium discoideum. a purified contact site a was digested with achromobacter protease i to produce a 31-kda fragment to which carbohydrate ii was mainly bound and a 21-kda fragment containing the nh2 terminus of contact site a, which was identified as ala-pro-thr-ile-thr-ala. the nh2 terminus of the 31-kda fragment was thr-glu-ala-thr-thr-ser. it was estimated ... | 1997 | 9024799 |
| nitrite reductase from achromobacter xylosoxidans forms stable trimers in dilute solution. | | 1997 | 9056938 |
| recurrent septicaemia due to "achromobacter group b". | | 1997 | 9138138 |
| d-lysine production from l-lysine by successive chemical racemization and microbial asymmetric degradation. | in order to develop a practical process for d-lysine production from l-lysine, successive chemical racemization and microbial asymmetric degradation were investigated. the racemization of l-lysine proceeded quantitatively at elevated temperatures. a sample of 1000 strains of bacteria, fungi, yeast and actinomyces were screened for the ability to degrade l-lysine asymmetrically. microorganisms belonging to the achromobacter, agrobacterium, candida, comamonas, flavobacterium, proteus, providencia, ... | 1997 | 9163947 |
| evidence for increased hydroxylation of pyrrolidone amino acid residues in the cuticle of mature onchocerca volvulus. | to determine whether morphologic changes are accompanied by variations in the biochemical and antigenic properties of the cuticle of onchocerca volvulus during development, we isolated and compared the 2-mercaptoethanol soluble cuticular proteins and the insoluble cuticlin from the predominant life-cycle stages occurring in man. sds-page analysis, before and after digestion with collagenase from achromobacter iophagus, revealed that the polypeptide composition of the 2-mercaptoethanol-solubilise ... | 1997 | 9197461 |
| intravenous line infection due to ochrobactrum anthropi (cdc group vd) in a normal host. | ochrobactrum anthropi, formerly known as achromobacter species (cdc group vd), is an aerobic, gram-negative bacillus widely distributed in aquatic environments. most important, it has been implicated as a cause of intravenous line infection in immunocompromised hosts with solid tumors or hematologic malignancies. trimethoprim-sulfamethoxazole and aminoglycosides are usually active against o. anthropi, but this organism is usually resistant to beta-lactam antibiotics. because o. anthropi is a low ... | 1997 | 9257145 |
| sulfated lipids as inhibitors of pancreatic trypsin and chymotrypsin in epithelium of the mammalian digestive tract. | cholesterol sulfate and i3so3-galcer, which were commonly contained in the epithelia of mammalian digestive tracts, were found to inhibit the activities of typical digestive enzymes, pancreatic trypsin and chymotrypsin, but steroid sulfates, gangliosides and the other membrane constituents did not show any inhibitory activity. the preincubation of trypsin with i3so3-galcer at 37 degrees c for 10min was necessary to inhibit the activity of trypsin, but cholesterol sulfate showed its inhibitory ac ... | 1997 | 9268697 |
| domon l, an immunopotentiating agent, acts as an nf-kappab activator. | domon l, a heat-treated component of gram-negative bacterium achromobacter stenohalis, is used for the treatment of infectious diseases of animals. here, we investigated the immunopotentiating potential of domon l. in vitro studies showed that domon l enhanced nitric oxide (no) formation from murine macrophage raw264.7 cells in concert with interferon (ifn)-gamma. the effect of domon l on nf-kappab activation was investigated, in order to understand the molecular mechanisms of enhanced no format ... | 1997 | 9271453 |
| characterization of the gene encoding catechol 2,3-dioxygenase from achromobacter xylosoxidans kf701. | catechol 2,3-dioxygenase (c23o) catalyzes a meta cleavage of the aromatic ring in catechol to form 2-hydroxymuconic semialdehyde. a c23o gene was cloned from chromosomal dna of a. xylosoxidans kf701, a soil bacterium degrading biphenyl, and expressed in e. coli hb101. in substrate specificity to catechol and its analogs, the c23o exhibited the highest aromatic ring-fission activity to catechol, and its relative activity to other dihydroxylated aromatics was 4-chlorocatechol > 4-methylcatechol > ... | 1997 | 9299526 |
| structure of a covalently cross-linked form of core histones present in the starfish sperm. | the post-translational modification of core histones plays an essential role in chromatin remodeling processes. we recently reported the occurrence of a novel histone modification, involving a epsilon-(gamma-glutamyl)lysine cross-link between a glutamine residue of histone h2b and a lysine residue of histone h4 in the testis of the starfish, asterina pectinifera[shimizu, t., hozumi, k., horiike, s., nunomura, k., ikegami, s., takao, t., and shimonishi, y. (1996) nature 380, 32]. in order to dete ... | 1997 | 9315845 |
| purification, characterization, and amino acid sequencing of dnase gamma from rat spleen. | an endonuclease named dnase gamma was purified to apparent homogeneity from rat splenocyte nuclei and its properties were characterized. we also determined the nh2-terminal and partial amino acid sequences of the proteolytic internal peptides. the molecular mass of gamma dnase was 33,000 daltons as determined by sds-polyacrylamide gel electrophoresis. a native molecular mass of 30,000 was estimated by gel filtration. purified dnase gamma is active in the presence of both ca2+ and mg2+ or mn2+ al ... | 1997 | 9328279 |
| using monoclonal antibodies to characterize a sequential epitope on the group i allergen of bermuda grass pollen. | cyn d 1, the group i allergen of bermuda grass pollen, had been purified and characterized. | 1997 | 9363907 |
| purification, staphylolytic activity, and cleavage sites of alpha-lytic protease from achromobacter lyticus. | alpha-lytic protease (alp) was purified from a bacteriolytic agent, achromopeptidase from achromobacter lyticus m497-1, and has been shown to possess staphylolytic activity. cleavage sites of this enzyme on the peptidoglycan of staphylococcus aureus were determined by n-terminal amino acid sequence and amino acid composition analyses. alp cleaved the n-acetylmuramoyl-l-alanine amide bond, the junction between the polysaccharide and peptide moieties, in addition to the d-ala-gly and gly-gly pepti ... | 1997 | 9399581 |
| bombyx mori nucleopolyhedrovirus encodes a dna-binding protein capable of destabilizing duplex dna. | a dna-binding protein (designated dbp) with an apparent molecular mass of 38 kda was purified to homogeneity from bmn cells (derived from bombyx mori) infected with the b. mori nucleopolyhedrovirus (bmnpv). six peptides obtained after digestion of the isolated protein with achromobacter protease i were partially or completely sequenced. the determined amino acid sequences indicated that dbp was encoded by an open reading frame (orf16) located at nucleotides (nt) 16189 to 17139 in the bmnpv genom ... | 1998 | 9525636 |
| achromobacter xylosoxidans bacteremia in patients with hematologic malignancies. | we report nine cases of achromobacter xylosoxidans bacteremia diagnosed in patients with hematologic malignancies. there was not an obvious epidemiologic link between cases and the organism was not isolated from any source. outcome was cure in all nine cases. in our experience, catheter removal is generally required for eradication of a. xylosoxidans. | 1998 | 9573685 |
| genetic structure of the bphg gene encoding 2-hydroxymuconic semialdehyde dehydrogenase of achromobacter xylosoxidans kf701. | 2-hydroxymuconic semialdehyde dehydrogenase catalyzes the conversion of 2-hydroxymuconic semialdehyde (hms) to an enol form of 4-oxalocrotonate which is a step in the catechol meta-cleavage pathway. a bphg gene encoding hms dehydrogenase of a. xylosoxidans kf701, a soil bacterium degrading biphenyl, was identified at between catechol 2,3-dioxygenase gene and hms hydrolase gene, and its sequence was analyzed. an open reading frame (orf) corresponding to bphg gene was consisted of 1461 nucleotides ... | 1998 | 9600061 |
| in search of circular permuted variants of escherichia coli dihydrofolate reductase. | a circularized form of a cys-free mutant of escherichia coli dihydrofolate reductase (dhfr) was used to search for a proteolytic site that gave new n- and c-termini on circularized dhfr with enzyme activity. of the six site-specific proteolytic enzymes tested, three proteases, achromobacter protease i (lysine-specific endopeptidase), asparaginylendopeptidase, and staphylococcus aureus v8 protease, cleaved a single site of the circularized dhfr to form circular permuted variants. twenty-four poss ... | 1998 | 9614709 |
| molecular cloning, expression, and site-directed mutagenesis of inorganic pyrophosphatase from thermus thermophilus hb8. | the genomic dna encoding the inorganic pyrophosphatase from an extremely thermophilic bacterium, thermus thermophilus hb8 (atcc27634), was isolated by colony hybridization with a probe designed as a part of gene amplified by the pcr method, which was derived from the partial amino acid sequence of the enzyme. the dna was cloned into a plasmid vector, puc118, after digestion with bamhi. the inserted nucleotide fragment was about 1.8 kbp in length and the nucleotide sequence included a 525 bp open ... | 1998 | 9644249 |
| the covalent structure of the blue copper-containing nitrite reductase from achromobacter xylosoxidans. | the complete amino acid sequence of the blue copper-containing nitrite reductase enzyme (nir) from achromobacter xylosoxidans has been determined by chemical analysis, supported by high precision mass analysis. the polypeptide chain contains 336 residues with an overall charge of 0, including the +2 state of each of the copper ions. the two nir enzymes for which the three-dimensional structures have been solved are green in color and have different absorption spectra than those of the blue-color ... | 1998 | 9647763 |
| isolation and characterization of rhamnose-binding lectins from eggs of steelhead trout (oncorhynchus mykiss) homologous to low density lipoprotein receptor superfamily. | two l-rhamnose-binding lectins named stl1 and stl2 were isolated from eggs of steelhead trout (oncorhynchus mykiss) by affinity chromatography and ion exchange chromatography. the apparent molecular masses of purified stl1 and stl2 were estimated to be 84 and 68 kda, respectively, by gel filtration chromatography. sodium dodecyl sulfate polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization time of flight mass spectrometry of these lectins revealed that stl1 was compo ... | 1998 | 9668106 |
| amino acid sequence of glutathione s-transferase rgstm5* from rat testis. | glutathione s-transferase rgstm5* was isolated from rat testis with a combination of glutathione affinity column and reverse-phase column chromatography. the protein was digested with achromobacter protease i or endoproteinase arg-c. the peptide fragments were isolated for electrospray ms and n-terminal peptide sequencing analyses. the primary amino acid sequence of rgstm5* comprises 217 residues and has a calculated average molecular mass of 25495.3 da. the result is identical to that obtained ... | 1998 | 9677335 |
| bacteriolytic activity and specificity of achromobacter beta-lytic protease. | achromobacter beta-lytic protease (blp), one of the bacteriolytic proteases secreted by achromobacter lyticus, exhibited both peptidase and bacteriolytic activities at alkaline ph. the protease was strongly inhibited by 1,10-phenanthroline, and one zinc atom was detected in the molecule by ion-spray mass spectrometry. the zinc-protease specifically cleaved gly-x bonds in peptides and possibly possessed subsites s2, s1, s1', and s2' for binding substrate [schecter, i. and berger, a. (1967) bioche ... | 1998 | 9685723 |
| isolation of broad-host-range replicons from marine sediment bacteria. | naturally occurring plasmids isolated from heterotrophic bacterial isolates originating from coastal california marine sediments were characterized by analyzing their incompatibility and replication properties. previously, we reported on the lack of dna homology between plasmids from the culturable bacterial population of marine sediments and the replicon probes specific for a number of well-characterized incompatibility and replication groups (p. a. sobecky, t. j. mincer, m. c. chang, and d. r. ... | 1998 | 9687436 |
| emendation of genus achromobacter and achromobacter xylosoxidans (yabuuchi and yano) and proposal of achromobacter ruhlandii (packer and vishniac) comb. nov., achromobacter piechaudii (kiredjian et al.) comb. nov., and achromobacter xylosoxidans subsp. denitrificans (rüger and tan) comb. nov. | based on the results of gc content determination and 16s rrna sequence analysis among the type strains of achromobacter xylosoxidans, 4 alcaligenes species, 5 bordetella species, and 12 species of 4 other genera, the separation of genus achromobacter yabuuchi and yano 1981, with the type species achromobacter xylosoxidans, is confirmed. alcaligenes ruhlandii (packer and vishniac) aragno and schlegel 1992 is a distinct species and not a senior synonym of achromobacter xylosoxidans. alcaligenes ru ... | 1998 | 9688077 |
| the nos (nitrous oxide reductase) gene cluster from the soil bacterium achromobacter cycloclastes: cloning, sequence analysis, and expression. | the nitrous oxide (n2o) reductase (nos) gene cluster from achromobacter cycloclastes has been cloned and sequenced. seven protein coding regions corresponding to nosr, nosz (structural n2o reductase gene), nosd, nosf, nosy, nosl, and nosx are detected, indicating a genetic organization similar to that of rhizobium meliloti. to aid homology studies, nosr from r. meliloti has also been sequenced. comparison of the deduced amino acid sequences with corresponding sequences from other organisms has a ... | 1998 | 9720302 |
| the c-terminal segment is essential for maintaining the quaternary structure and enzyme activity of the nitric oxide forming nitrite reductase from achromobacter cycloclastes. | we have constructed and expressed a series of mutated nitrite reductase (nir) mutants based on the sequence of nir from achromobacter cycloclastes. deleting a pentapeptide, an undecapeptide, or a heptadecapeptide from the c-terminus of nir resulted in a series of c-terminal deletion mutated proteins designated as nir-5, nir-11, and nir-17, respectively. a c-terminally extended mutated protein, nir+8, was also produced, which contains an extra octapeptide attached to the c-terminus of the wild-ty ... | 1998 | 9784423 |
| [two cases of achromobacter xylosoxidans sepsis]. | achromobacter xylosoxidans is a gram-negative bacterium whose natural habitat has not been clearly defined. it has been isolated from ear discharge and the large intestine of humans and from various hospital or environmental water sources. infection with a. xylosoxidans in humans has been documented, and resulting illnesses include meningitis, pneumonia, cholecystitis, peritonitis and urinary tract infection. bacteremia due to a. xylosoxidans is rare, and little information on treatment is avail ... | 1998 | 9847526 |
| cloning of a lysobacter enzymogenes gene that encodes an arginyl endopeptidase (endoproteinase arg-c). | screening an expression library of lysobacter enzymogenes dna allowed us to clone a gene encoding a serine protease that cleaves synthetic substrates c-terminal to arg and, to a lesser extent, lys residues. the gene product, which shares sequence homology with the lysyl endopeptidases from l. enzymogenes and achromobacter lyticus, consists of a signal sequence (24 residues), pro-region ( approximately 195 residues), and catalytic domain ( approximately 244 residues). downstream of this gene is a ... | 1998 | 9878833 |
| prepro-leaders lacking n-linked glycosylation for secretory expression in the yeast saccharomyces cerevisiae. | synthetic prepro-leaders lacking consensus n-linked glycosylation sites confers secretion competence of correctly folded insulin precursor expressed in the yeast species saccharomyces cerevisiae with a yield comparable to, or better than the alpha-factor prepro-leader. in contrast, the s. cerevisiae alpha-factor prepro-leader's three n-linked oligosaccharide chains are necessary for the ability to facilitate secretion of the insulin precursor from s. cerevisiae (t. kjeldsen et al., biotechnol. a ... | 1998 | 9882564 |
| an attempt to use selected strains of bacteria adapted to high concentrations of petroleum oil to increase the effective removal of petroleum products in excess activated sludge in laboratory conditions. | forty two strains of bacteria were isolated from excess activated sludge from petroleum wastewater treatment plant. the strains were identified and classified to the following groups: enterobacteriaceae (7 strains), anitratum (3 strains), pseudomonas (13 strains), micrococcus (12 strains), comamonas (2 strains), xanthomonas (2 strains), achromobacter (1 strain) and vibrio-aeromonas (1 strain). one of the isolates was found to be a yeast strain. following preliminary selection ten strains, showin ... | 1998 | 9990713 |
| analysis of the nitrous oxide reduction genes, noszdfyl, of achromobacter cycloclastes. | the structural gene, nosz, for the monomeric n2o reductase has been cloned and sequenced from the denitrifying bacterium achromobacter cycloclastes. the nosz gene encodes a protein of 642 amino acid residues and the deduced amino acid sequence showed homology to the previously derived sequences for the dimeric n2o reductases. the relevant dna region of about 3.6 kbp was also sequenced and found to consist of four genes, nosdfyl based on the similarity with the n2o reduction genes of pseudomonas ... | 1998 | 10048486 |
| cloning, sequencing, and transcriptional studies of the gene encoding copper-containing nitrite reductase from alcaligenes xylosoxidans ncimb 11015. | gene encoding of the blue copper-containing nitrite reductase (nir) from alcaligenes xylosoxidans ncimb 11015 has been cloned and characterized. the nir is translated into a polypeptide of 360 amino acid residues as a precursor, and the n-terminal 24 residues are subsequently removed upon transport into the periplasm as a mature protein. a specific transcription product of nir was detected only in the presence of nitrate. the aeration level of the culture medium did not show a significant effect ... | 1999 | 10049725 |
| a monoclonal antibody, 3a10, recognizes a specific amino acid sequence present on a series of developmentally expressed brain proteins. | immunoblotting showed that a monoclonal antibody, 3a10, binds to a series of rat brain-specific antigens with molecular masses of 150-, 120-, 118-, 106-, 104-, 79-, and 77-kda. the expression of 3a10 antigens is dependent on the developmental stage of the brain; only the 106-kda antigen is detected during embryonic stages of rat brain development, while the expression of the remaining 6 antigens starts after birth and reaches a maximum during postnatal days 15-21. detection of the 3a10 antigens ... | 1999 | 10050030 |
| ochrobactrum anthropi bacteremia. | ochrobactrum anthropi (o. anthropi ), formerly known as achromobacter cdc group vd, is a gram-negative bacillus that is aerobic, oxidase producing, and nonlactose fermenting. this organism has been found in environmental and hospital water sources and has pathogenic potential in humans. most reports in the literature of o. anthropi bacteremia are associated with intravenous line infections. we describe a case of bacteremia with o. anthropi in a 33-month-old boy with acute osteomyelitis. o. anthr ... | 1999 | 10076115 |
| structure of the glucan-binding sugar chain of tip1p, a cell wall protein of saccharomyces cerevisiae. | tip1p is one of the major cell wall mannoproteins of saccharomyces cerevisiae and is presumed to be synthesized as a glycosylphosphatidylinositol (gpi)-anchored form. we purified tip1p from a glucanase extract of yeast cell walls and analyzed the sugar chain involved in the cell wall linkage. one mol of glucanase-extracted tip1p contained 7.5 mol of glucose derived from glucan and 1 mol of ethanolamine, a component of the gpi anchor. one mol of the c-terminal peptide of tip1p digested with achro ... | 1999 | 10216230 |
| crystal structure determinations of oxidized and reduced pseudoazurins from achromobacter cycloclastes. concerted movement of copper site in redox forms with the rearrangement of hydrogen bond at a remote histidine. | the crystal structures of oxidized and reduced pseudoazurins from a denitrifying bacterium, achromobacter cycloclastes iam1013, have been determined at 1.35- and 1.6-a resolutions, respectively. the copper site in the oxidized state exhibits a distorted tetrahedral structure like those of other pseudoazurins. however, not only a small change of the copper geometry, but concerted peptide bond flips are identified. the imidazole ring of remote his6 has a hydrogen bonding distance of 2.73 a between ... | 1999 | 10364229 |
| lung infections in pediatric lung transplantation: experience in 49 cases. | pulmonary infections, and particularly cytomegalovirus (cmv) infections, are a major cause of morbidity after lung transplantation. we report here our results in 49 pediatric lung transplantations. | 1999 | 10371127 |
| amino acid sequence and some properties of phytolacain r, a cysteine protease from full-growth fruits of pokeweed, phytolacca americana. | a cysteine protease, phytolacain r from full-growth greenish fruits of pokeweed, phytolacca americana l, was purified to electrophoretic homogeneity by a simple purification procedure employing cm-sepharose ion-exchange chromatography. the enzyme was present in low content in the young fruits about 50 d after flowering but gradually accumulated in growing fruits. its molecular mass was estimated to be ca. 23 kda by sds-page, and its sugar content was zero. its amino acid sequence was established ... | 1999 | 10393317 |
| production of homo- and hetero-dimeric isozymes from two aldehyde oxidase genes of arabidopsis thaliana. | polyclonal antibodies were raised against synthetic peptides or recombinant polypeptides encoded by arabidopsis atao-1 and atao-2 cdnas, which have sequences similar to maize and animal aldehyde oxidase (ao) cdnas. anti-atao-1 antibodies recognized aoalpha and aobeta among the three isoforms, aoalpha, aobeta, and aogamma, detected in arabidopsis seedlings after native page, while anti-atao-2 antibodies reacted with aobeta and aogamma. the polypeptide specifically recognized by each antibody was ... | 1999 | 10423535 |
| the ph-dependent changes of intramolecular electron transfer on copper-containing nitrite reductase. | electron transfer over 12.6 a from the type 1 copper (t1cu) to the type 2 copper (t2cu) was investigated in the copper-containing nitrite reductases from two denitrifying bacteria (alcaligenes xylosoxidans gifu 1051 and achromobacter cycloclastes ian 1013), following pulse radiolytical reduction of t1cu. in the presence of nitrite, the rate constant for the intramolecular electron transfer of the enzyme from a. xylosoxidans decreased 1/2 fold to 9 x 10(2) s-1 (20 degrees c, ph 7.0) as compared t ... | 1999 | 10423537 |
| batch culture biodegradation of methylhydrazine contaminated nasa wastewater. | the batch culture degradation of nasa wastewater containing mixtures of citric acid, methylhydrazine, and their reaction product was studied. the organic contaminants present in the nasa wastewater were degraded by achromobacter sp., rhodococcus b30 and rhodococcus j10. while the achromobacter sp. showed a preference for the degradation of the citric acid, the rhodococcus species were most effective in reducing the methylhydrazine and the reaction product. removals of more than 50% were observed ... | 1999 | 10466199 |
| reactivity of lys(nh2)-containing peptides toward endopeptidases. | lys(nh2)-containing peptides were subjected to various proteolytic enzymes which were selected for their well-documented specificity for arginyl and/or lysyl peptide bonds. lys(nh2)-containing peptides were cleaved more rapidly by clostripain than the corresponding lysyl peptides. on the other hand, they proved to be resistant to achromobacter protease i hydrolysis. the modified peptides synthesized in this study were more stable than the arginyl and lysyl analogues when incubated with trypsin o ... | 1999 | 10507684 |
| helix 6 of subdomain iii a of human serum albumin is the region primarily photolabeled by ketoprofen, an arylpropionic acid nsaid containing a benzophenone moiety. | it is well known that the subdomain iii a (site ii) of human serum albumin (hsa) binds a variety of endogenous and exogenous substances. however, the nature of the microenvironment of the binding site remains unclear. ketoprofen (kp), an arylpropionic acid nsaid which contains a benzophenone moiety, was used as a photoaffinity labeling agent to label the binding region. subsequent cnbr cleavage of the photolabeled hsa revealed that the 11.6 kda and 9.4 kda fragments contained most of the incorpo ... | 1999 | 10556556 |
| the subunit structure of nitrite reductase purified from the denitrifier achromobacter cycloclastes. | the copper-containing nitrite reductase of achromobacter cycloclastes has been considered to be a homotrimer with three identical subunits both in the crystal and in solution. in this study, however, the enzyme was found to be a heterotrimer consisting of two subunits with molecular masses of 37 kda and 36.2 kda, and the 37 kda subunit was 6 amino acid residues longer than the smaller subunit. signal-peptide cleavage sites in its n-terminal region are discussed. | 1999 | 10635570 |
| [twenty-seven years with the nomenclature of achromobacter xylosoxidans]. | | 1999 | 10651522 |
| stimulation of the ionic transport system in brassica napus by a plant growth-promoting rhizobacterium (achromobacter sp.). | a plant growth-promoting rhizobacterium belonging to the genus achromobacter was isolated from the oil-seed-rape (brassica napus) root. growth promotion bioassays were performed with oilseed rape seedlings in a growth chamber in test tubes containing attapulgite and mineral nutrient solution, containing no3- as n source. the presence of this achromobacter strain increased shoot and root dry weight by 22-33% and 6-21%, respectively. inoculation of young seedlings with the achromobacter bacteria i ... | 2000 | 10749536 |
| purification, characterization, and primary structure of a novel cell wall hydrolytic amidase, cwha, from achromobacter lyticus. | a novel bacteriolytic enzyme cwha (cell wall hydrolytic amidase) was purified by ion exchange and gel-filtration chromatographies from a commercial bacteriolytic preparation from achromobacter lyticus. cwha exhibited optimal ph at 8.5 and lysed chcl(3)-treated escherichia coli more efficiently than micrococcus luteus, staphylococcus aureus, enterococcus faecalis, and pediococcus acidilactici. the enzyme was inhibited by 1,10-phenanthroline strongly and by edta to a lesser extent, suggesting that ... | 2000 | 10833271 |
| isolation and characterization of an achromobacter xylosoxidans strain b3 and other bacteria capable to degrade the synthetic chelating agent iminodisuccinate. | three bacterial strains were isolated, which used the synthetic chelating agent iminodisuccinate (ids) as sole carbon source for growth in mineral salts media (msm). taxonomic analysis and 16s rdna sequence analysis identified one of these isolates (b3), which was isolated from sewage sludge, as a strain of achromobacter xylosoxidans subsp. xylosoxidans. it exhibited a doubling time of approximately 3 h in liquid msm supplemented with ids and grew even in the presence of 1.0% (w/v) ids. since ph ... | 2000 | 10867232 |
| profiling of caenorhabditis elegans proteins using two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight-mass spectrometry. | the nematode caenorhabditis elegans (c. elegans) is the first animal whose whole 97 mb genome sequence, encoding ca. 19000 open reading frames (orf's), has been essentially determined. we tried to establish a 2-de map of the nematode proteome by means of two-dimensional polyacrylamide gel electrophoresis (2-d page). a soluble protein fraction of mixed stages of the worm, wild-type strain n2, was applied to 2-d page. after coomassie brilliant blue (cbb) staining, 1200 spots were detected and 140 ... | 2000 | 10870962 |
| isolation and identification of cellulolytic bacteria involved in the degradation of natural cellulosic fibres. | in search for bacterial cultures that are able to rapidly degrade cellulosic plant fibres in vitro, 77 cellulolytic strains were isolated from belgian and czech soils after enrichment on flax or sisal fibres as sole sources of carbon. the strains were characterized using fatty acid analysis, and 74 strains were grouped into three major clusters by numerical analysis. the first major cluster contained cellulomonas strains. within this cluster three subclusters could be delineated by principal com ... | 2000 | 10930083 |
| zeta-crystallin catalyzes the reductive activation of 2,4,6-trinitrotoluene to generate reactive oxygen species: a proposed mechanism for the induction of cataracts. | exposure to 2,4,6-trinitrotoluene (tnt) has been shown to cause induction of cataract in which oxidative stress plays a critical role. from bovine lens we purified to homogeneity and identified an enzyme that catalyzes the reduction of tnt, resulting in the production of reactive oxygen species. the final preparation of tnt reductase showed a single band with a subunit molecular weight of 38 kda on sds-page. sequence data from peptides obtained by digestion with lysylendopeptidase achromobacter ... | 2000 | 10930585 |
| complete amino acid sequence of japanese chestnut agglutinin. | the complete amino acid sequence of japanese chestnut (castanea crenata sieb. et zucc.) agglutinin (cca) was determined. analysis by sims of the acidic peptide obtained by pepsin digestion revealed that the n-terminal amino acid sequence should be acetyl-met-glu-glu. prior to sequence analysis, redetermination of cysteine residues indicated the presence of one cysteine residue per subunit. the complete sequence was determined by endoproteinase arg-c and achromobacter protease i digestion, and cn ... | 2000 | 11006076 |
| analysis of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pest4011 of achromobacter xylosoxidans subsp. denitrificans strain est4002. | the 2,4-dichlorophenoxyacetic acid (2,4-d)-degradative bacterium achromobacter xylosoxidans subsp. denitrificans strain est4002, isolated in estonia more than 10years ago, was found to contain the 70kb plasmid pest4011 that is responsible for the bacterium having had obtained a stable 2,4-d(+) phenotype. the tfd-like genes for 2, 4-d degradation of the strain est4002 were located on a 10.5kb region of pest4011, but without functional genes coding for chloromuconate cycloisomerase and chlorodiene ... | 2000 | 11024288 |
| amino acid sequence and some properties of phytolacain g, a cysteine protease from growing fruit of pokeweed, phytolacca americana. | a protease, phytolacain g, has been found to appear on cm-sepharose ion-exchange chromatography of greenish small-size fruits of pokeweed, phytolacca americana l, from ca. 2 weeks after flowering, and increases during fruit enlargement. reddish ripe fruit of the pokeweed contained both phytolacain g and r. the molecular mass of phytolacain g was estimated to be 25.5 kda by sds-page. its amino acid sequence was reconstructed by automated sequence analysis of the peptides obtained after cleavage w ... | 2000 | 11042392 |
| recurrent achromobacter xylosoxidans bacteremia associated with persistent lymph node infection in a patient with hyper-immunoglobulin m syndrome. | achromobacter xylosoxidans (formerly alcaligenes xylosoxidans) is a rare but important cause of bacteremia in immunocompromised patients, and strains are usually multiply resistant to antimicrobial therapy. we report an immunocompromised patient with hyper-immunoglobulin m syndrome who suffered from 14 documented episodes of a. xylosoxidans bacteremia. each episode was treated and resulted in rapid clinical improvement, with blood cultures testing negative for bacteria. between episodes, a. xylo ... | 2000 | 11073750 |
| spectroscopic and electrochemical properties of the met86gln mutant of achromobacter cycloclastes pseudoazurin. | the mutant replacing the met86 ligand of achromobacter cycloclastes pseudoazurin (ac-paz) with gln has been prepared and spectroscopically and electrochemically characterized. ac-paz has four ligands (2his, cys, and met) and donates one electron to its cognate cu-containing nitrite reductase (ac-nir). the mutant ([met86gln]paz) shows the electronic absorption and cd spectra considerably similar to those of zucchini mavicyanin (mv) and lacquer and cucumber stellacyanins (st) having 2his, cys, and ... | 2000 | 11132642 |
| recurrent achromobacter piechaudii bacteremia in a patient with hematological malignancy. | we describe a recurrent bacteremia caused by achromobacter (formerly alcaligenes) piechaudii in association with an intravenous catheter in an immunocompromised 73-year-old man. this is the first reported case of bacteremia due to a. piechaudii. | 2001 | 11158159 |
| molecular engineering of myoglobin: the improvement of oxidation activity by replacing phe-43 with tryptophan. | the f43w and f43w/h64l myoglobin (mb) mutants have been constructed to investigate effects of an electron rich oxidizable amino acid residue in the heme vicinity on oxidation activities of mb. the phe-43 --> trp mutation increases the rate of one-electron oxidation of guaiacol by 3-4-fold; however, the peroxidase activity for f43w/h64l mb is less than that of the f43w single mutant because the absence of histidine, a general acid-base catalyst, in the distal heme pocket suppresses compound i for ... | 2001 | 11170427 |
| plastid-expressed 5-enolpyruvylshikimate-3-phosphate synthase genes provide high level glyphosate tolerance in tobacco. | plastid transformation (transplastomic) technology has several potential advantages for biotechnological applications including the use of unmodified prokaryotic genes for engineering, potential high-level gene expression and gene containment due to maternal inheritance in most crop plants. however, the efficacy of a plastid-encoded trait may change depending on plastid number and tissue type. we report a feasibility study in tobacco plastids to achieve high-level herbicide resistance in both ve ... | 2001 | 11208018 |
| in70 of plasmid pax22, a bla(vim-1)-containing integron carrying a new aminoglycoside phosphotransferase gene cassette. | an achromobacter xylosoxydans strain showing broad-spectrum resistance to beta-lactams (including carbapenems) and aminoglycosides was isolated at the university hospital of verona (verona, italy). this strain was found to produce metallo-beta-lactamase activity and to harbor a 30-kb nonconjugative plasmid, named pax22, carrying a bla(vim-1) determinant inserted into a class 1 integron. characterization of this integron, named in70, revealed an original array of four gene cassettes containing, r ... | 2001 | 11257042 |
| [achromobacter xylosoxidans bacteremia in a patient with community-acquired pneumonia]. | achromobacter xylosoxidans is a rare cause of bacteremia, and little information on treatment is available. the majority of patients who have developed achromobacter bacteremia have presented predisposing causes to the infection. a case of community-acquired pneumonia and bacteremia due to a. xylosoxidans in a previously healthy patient is reported. achromobacter is usually resistant to ampicillin, cephalosporins (1st, 2nd, and 3rd generation), aminoglycosides, and fluoroquinolones. piperacillin ... | 2001 | 11265630 |
| nreb from achromobacter xylosoxidans 31a is a nickel-induced transporter conferring nickel resistance. | there are two distinct nickel resistance loci on plasmid ptom9 from achromobacter xylosoxidans 31a, ncc and nre. expression of the nreb gene was specifically induced by nickel and conferred nickel resistance on both a. xylosoxidans 31a and escherichia coli. e. coli cells expressing nreb showed reduced accumulation of ni(2+), suggesting that nreb mediated nickel efflux. the histidine-rich c-terminal region of nreb was not essential but contributed to maximal ni(2+) resistance. | 2001 | 11292799 |
| isolation and partial characterization of a 46-kd allergen of bermuda grass pollen. | cyn d bd46k, a 46-kd component of bermuda grass (cynodon dactylon) pollen, had been identified as an allergenic constituent. in the present study two-dimensional (2d) gel electrophoresis illustrated the presence of five acidic isoforms in cyn d bd46k, and this molecule was purified by monoclonal antibody (mab) affinity chromatography for further characterization. using a digoxigenin-labeled lectin-binding assay, the elucidating protein was disclosed to be a glycoprotein with terminal mannose. th ... | 2001 | 11455197 |
| oxidative modification of tryptophan 43 in the heme vicinity of the f43w/h64l myoglobin mutant. | the f43w/h64l myoglobin mutant was previously constructed to investigate the effects of electron-rich tryptophan residue in the heme vicinity on the catalysis, where we found that trp-43 in the mutant was oxidatively modified in the reaction with m-chloroperbenzoic acid (mcpba). to identify the exact structure of the modified tryptophan in this study, the mcpba-treated f43w/h64l mutant has been digested stepwise with lys-c achromobacter and trypsin to isolate two oxidation products by preparativ ... | 2001 | 11481319 |
| bordetella petrii sp. nov., isolated from an anaerobic bioreactor, and emended description of the genus bordetella. | a novel bordetella species was isolated from an anaerobic, dechlorinating bioreactor culture enriched from river sediment. the only strain, se-1111r(t) (= dsm 12804t = ccug 43448t), for which the name bordetella petrii is proposed, is designated the type strain of the novel species. strain se-1111r(t) was isolated from the dechlorinating mixed culture due to its ability to anaerobically reduce selenate to elemental selenium. comparative 16s rdna sequence analysis showed a close relationship betw ... | 2001 | 11491321 |
| conversion of gamma-butyrobetaine to l-carnitine by achromobacter cycloclast. | l-carnitine is an ubiquitous substance that plays a major role in the transportation of long-chain fatty acids. we investigated crucial factors that influence microbial conversion of gamma-butyrobetaine to l-carnitine using an achromobacter cycloclast strain. two-stage culture results showed that gamma-butyrobetaine induced enzymes essential for the conversion, which suggests that the precursor should be present in the initial cell growth stage. the addition of yeast extract enhanced l-carnitine ... | 2001 | 11494108 |
| isolation and characterization of achromobacter xylosoxidans t7 capable of degrading toluidine isomers. | a bacterial strain capable of utilizing toluidine isomers as its sole source of carbon and energy for growth was isolated from contaminated soil. the isolate was identified as achromobacter xylosoxidans and was designated strain t7. strain t7 differs from other toluidine-degrading strains with respect to the use of all three toluidine isomers even as an equimolar mixture. additionally, strain t7 harbours the ability to use aniline, phenol, and cresols as growth substrates. utilization of the tol ... | 2001 | 11512448 |
| identity and potential functions of heterotrophic bacterial isolates from a continuous-upflow fixed-bed reactor for denitrification of drinking water with bacterial polyester as source of carbon and electron donor. | a collection of 186 heterotrophic bacteria, isolated directly from a continuous-upflow fixed-bed reactor for the denitrification of drinking water, in which poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (phbv) granules acted as biofilm carrier, carbon source and electron donor, was studied with regard to taxonomic affiliation and degradation and denitrification characteristics. two granule samples were taken from a fully operating reactor for enumeration and isolation of heterotrophic bacteria. o ... | 2001 | 11518336 |
| identification of strains of alcaligenes and agrobacterium by a polyphasic approach. | the number of stable discriminant biochemical characters is limited in the genera alcaligenes and agrobacterium, whose species are consequently difficult to distinguish from one another by conventional tests. moreover, genomic studies have recently drastically modified the nomenclature of these genera; for example, alcaligenes xylosoxidans was transferred to the genus achromobacter in 1998. twenty-five strains of achromobacter xylosoxidans, three strains of an agrobacterium sp., five strains of ... | 2001 | 11526136 |
| cathelicidin peptides inhibit multiply antibiotic-resistant pathogens from patients with cystic fibrosis. | endogenous peptide antibiotics are under investigation as inhaled therapeutic agents for cystic fibrosis (cf) lung disease. the bactericidal activities of five cathelicidin peptides (ll37 [human], cap18 [rabbit], mcramp [mouse], rcramp [rat], and smap29 [sheep]), three novel alpha-helical peptides derived from smap29 and termed ovispirins (ov-1, ov-2, and ov-3), and two derivatives of cap18 were tested by broth microdilution assays. their mics were determined for multiply antibiotic-resistant ps ... | 2001 | 11557478 |
| use of random amplified polymorphic dna pcr to examine epidemiology of stenotrophomonas maltophilia and achromobacter (alcaligenes) xylosoxidans from patients with cystic fibrosis. | stenotrophomonas maltophilia and achromobacter (alcaligenes) xylosoxidans have been increasingly recognized as a cause of respiratory tract colonization in cystic fibrosis (cf). although both organisms have been associated with progressive deterioration of pulmonary function, demonstration of causality is lacking. to examine the molecular epidemiology of s. maltophilia and a. xylosoxidans in cf, isolates from patients monitored for up to 2 years were fingerprinted using a pcr-based randomly ampl ... | 2001 | 11574579 |
| pigmentiphaga kullae gen. nov., sp. nov., a novel member of the family alcaligenaceae with the ability to decolorize azo dyes aerobically. | the taxonomic position of pseudomonas strain k24, which was isolated previously after an aerobic enrichment with the azo compound 1-(4'-carboxyphenylazo)-4-naphthol as the sole source of carbon and energy, was investigated. the detection of a quinone system with ubiquinone q-8 as the predominant compound and a polyamine pattern with putrescine and 2-hydroxyputrescine as the major polyamines present suggested that strain k24t belongs to the beta-subclass of the proteobacteria. this was supported ... | 2001 | 11594620 |
| [use of pseudomonas and achromobacter species bacteria--degraders of surface-active agents--for detection and destruction of polycyclic aromatic hydrocarbons]. | the developed biosensor models were based on the use of immobilized pseudomonas and achromobacter cells for polycyclic aromatic hydrocarbons and surfactants detection. the responses of biosensors based on bacteria-degraders of anionic surfactants for organic substrates, which related to different classes of surfactants, aromatic and policyclic aromatic hydrocarbons (pah) were investigated. the sensor showed the highest sensitivity to anionic surfactants and pah. the lower limit of sodium dodecyl ... | 2001 | 11599420 |
| investigation of a peptide responsible for amyloid fibril formation of beta 2-microglobulin by achromobacter protease i. | to obtain insight into the mechanism of amyloid fibril formation from beta(2)-microglobulin (beta2-m), we prepared a series of peptide fragments using a lysine-specific protease from achromobacter lyticus and examined their ability to form amyloid fibrils at ph 2.5. among the nine peptides prepared by the digestion, the peptide ser(20)-lys(41) (k3) spontaneously formed amyloid fibrils, confirmed by thioflavin t binding and electron microscopy. the fibrils composed of k3 peptide induced fibril fo ... | 2002 | 11687582 |
| enhanced biodegradation of methylhydrazine and hydrazine contaminated nasa wastewater in fixed-film bioreactor. | the aerobic biodegradation of national aeronautics and space administration (nasa) wastewater that contains mixtures of highly concentrated methylhydrazine/hydrazine, citric acid and their reaction product was studied on a laboratory-scale fixed film trickle-bed reactor. the degrading organisms, achromobacter sp., rhodococcus b30 and rhodococcus j10, were immobilized on coarse sand grains used as support-media in the columns. under continuous flow operation, rhodococcus sp. degraded the methylhy ... | 2001 | 11693290 |
| preparation of fab' from murine igg2a for thiol reactive conjugation. | lysyl endopeptidase (le) from achromobacter lyticus m497-1 (ec 3.4.21.50) was utilized to prepare f(ab')2 fragments from mouse anti-p-glycoprotein igg2a obtained from the uic2 hybridoma. this report describes a novel single step purification procedure for f(ab')2 fragments that eliminates residual le activity responsible for secondary cleavage of f(ab')2 to fab fragments. the purification of f(ab')2 and fc fragments was accomplished utilizing protein g affinity chromatography and either gradient ... | 2001 | 11697031 |
| alkanesulfonate degradation by novel strains of achromobacter xylosoxidans, tsukamurella wratislaviensis and rhodococcus sp., and evidence for an ethanesulfonate monooxygenase in a. xylosoxidans strain ae4. | novel isolates of achromobacter xylosoxidans, tsukamurella wratislaviensis and a rhodococcus sp. are described. these grew with short-chain alkanesulfonates as their sole source of carbon and energy. t. wratislaviensis strain sb2 grew well with c(3)-c(6) linear alkanesulfonates, isethionate and taurine, rhodococcus sp. strain cb1 used c(3)-c(10) linear alkanesulfonates, taurine and cysteate, but neither strain grew with ethanesulfonate. in contrast, a. xylosoxidans strain ae4 grew well with etha ... | 2001 | 11734883 |
| purification and identification of monoubiquitin-phosphoglycerate mutase b complex from human colorectal cancer tissues. | ubiquitin-conjugated proteins in human colorectal cancer tissues were analyzed by the immunoprecipitation with the antibody fk2 against conjugated ubiquitin followed with sds-page. in these immunoprecipitable proteins, a 38-kda protein was abundant in the tumor regions but almost absent in the adjacent normal regions in 17/26 patients, thus we attempted to purify it. using immunoaffinity chromatography with the antibody fk2 followed by gel filtration and sds-page, approximately 10 pmol of this p ... | 2001 | 11745460 |
| [isolation and selection of strains used to degrade organic chlorine pesticides and application effects]. | with the organic chlorine pesticides (666, ddt) as sole carbon resources, three strains, no. 153 (bacillus), no. 411 (achromobacter) and no. 512 (pseudomonas), which can degrade 666 (bhc), were isolated and selected in tonomura culture medium. the degradation rates of these strains were 59.6%, 56.9% and 56.0% for total amount of 666, and 55.9%, 57.6% and 56.9% for beta-666, respectively. the other strains, no. 288 (alcaligenes), no. 410 and no. 411 (achromobacter) to degrade ddt were also obtain ... | 2000 | 11767607 |
| effect of histidine 6 protonation on the active site structure and electron-transfer capabilities of pseudoazurin from achromobacter cycloclastes. | the paramagnetic (1)h nmr spectrum of cu(ii) pseudoazurin [pacu(ii)] contains eight directly observed hyperfine-shifted resonances which we have assigned using saturation transfer experiments on a 1:1 mixture of pacu(i) and pacu(ii). the spectrum exhibits a number of similarities to those of other cupredoxins, but differences are found concerning the cu-s(met) interaction. the spectrum is dependent on ph* in the range 8.5-4.5 (pk(a)* 6.4), and a conformational change involving movement of the co ... | 2002 | 11772009 |
| comparison of two culture methods for detection of tobramycin-resistant gram-negative organisms in the sputum of patients with cystic fibrosis. | a culture method utilizing quantitative plating on antibiotic-containing media has been proposed as a technique for the detection of tobramycin-resistant organisms that is more sensitive than standard methods. typical sputum culture methods quantitate the relative amounts of each distinct morphotype, followed by antibiotic susceptibility testing of a single colony of each morphotype. sputum specimens from 240 cystic fibrosis patients were homogenized, serially diluted, and processed in parallel ... | 2002 | 11773088 |
| contribution of an imidazole-indole stack to high catalytic potency of a lysine-specific serine protease, achromobacter protease i. | achromobacter protease i (api), a lysine-specific serine-protease of the trypsin family, has an aromatic-ring stacking trp 169-his 210 in close proximity to the reactive site. in order to investigate the role of this novel aromatic stacking, several mutants of the two residues were constructed and their kinetic parameters were determined. three his 210 mutants showed lower activity by one order of magnitude than the wild-type with a peptide substrate of ala-ala-lys-mca (4-methylcoumaryl-7-amide) ... | 2002 | 11820934 |
| brackiella oedipodis gen. nov., sp. nov., gram-negative, oxidase-positive rods that cause endocarditis of cotton-topped tamarin (saguinus oedipus). | a gram-negative, oxidase-positive, rod-shaped bacterium isolated from the heart of a cotton-topped tamarin was characterized by 16s rdna sequence analysis, sds-page of whole-cell proteins, fatty acid analysis and biochemical tests. outer-membrane proteins, iron-regulated outer-membrane proteins, lipopolysaccharides and siderophore production were studied. on the basis of the results, the organism belongs to the beta-proteobacteria where it forms a separate line of descent, for which a novel genu ... | 2002 | 11837301 |