the e-test and campylobacter jejuni. | the e-test is a recently introduced method for performing antimicrobial susceptibility tests. we compared the e-test to the broth microdilution test and to the standard agar dilution test by using five antimicrobial agents tested against 55 clinical isolates of campylobacter jejuni from 11 locations in usa (group 1). later, we selected 30 strains (group 2), which were more resistant than the original survey isolates. erythromycin, tetracycline, and ciprofloxacin were tested on both groups of org ... | 1992 | 1643827 |
lack of colonization of 1 day old chicks by viable, non-culturable campylobacter jejuni. | seven strains of campylobacter jejuni, isolated from various sources [human (n = 2), chicken (n = 3), water (n = 2)], were studied under starvation conditions in filter-sterilized and pasteurized surface water by acridine orange direct count (aodc), viable count (dvc) and culture methods. plate counts showed a rapid decline (2 log-units/day) for all strains under these conditions. only one of the seven strains (14%) showed a (prolonged) viable, non-culturable 'state'. the ability of these viable ... | 1992 | 1644708 |
[surveillance study on bacteriological etiology of 8371 children with diarrhea]. | the results of stool cultures from 8371 children of 0-13 years old with diarrhea during the years of january 1981 to december 1989 in shanghai area are reported. 2906 strains of enteropathogenic bacteria were isolated, with total detection rate of 34.72%. shigella were still the most common pathogens in 1981-1984, and campylobacter jejuni took second place, whereas from 1985 on campylobacter exceeded shigella as the first pathogenic bacteria of children's diarrhea. except in 1987 and 1988 shigel ... | 1991 | 1647837 |
characterization of high-level quinolone resistance in campylobacter jejuni. | high-level resistance to quinolones has previously been shown to occur in campylobacter spp. both in vitro and in patients treated with quinolones. we have selected isolates that are resistant to quinolones by plating cells from a susceptible c. jejuni strain, ua535, on medium containing nalidixic acid at 32 micrograms/ml. fluctuation analysis indicated that resistance occurred by mutation at a frequency of 5 x 10(-8) per cell plated. unlike what is observed with other gram-negative organisms, t ... | 1991 | 1649570 |
antigenic shifts in serotype determinants of campylobacter coli are accompanied by changes in the chromosomal dna restriction endonuclease digestion pattern. | changes in somatic (o) lipopolysaccharide (lps) antigenic specificities of campylobacter coli serostrains were observed after continuous laboratory subculture. two serostrains (c. coli o34 and c. coli o48) lost o specificity and did not react with homologous or any of the available heterologous antisera. the c. coli serostrain for serogroup o5, after subculture, yielded a variant that had acquired a new specificity which was detectable with a heterologous antiserum. in a repeat experiment with t ... | 1991 | 1654431 |
canine coronavirus infection in the dog following oronasal inoculation. | the pathogenesis of canine coronavirus (ccv) infection in 10-week-old puppies was studied up to 14 days after oronasal inoculation. mild diarrhoea was seen from three to 11 days after inoculation, approximately coincident with faecal virus shedding. virus was initially isolated from the tonsils on day 3, and then from both small and large intestinal tissues up to 14 days after inoculation. virus was also isolated from liver and lung. histological changes were not seen in any tissues, but ccv ant ... | 1991 | 1654584 |
[isolation of enteropathogenic microorganism from patients with infection of the digestive tract during 1976 to 1988 in tenri hospital]. | enteropathogenic microorganisms isolated from feces of 9,393 patients with diarrhea or enteritis in our hospital between 1976 and 1988 were analyzed. as the result of the examination of 5,443 outpatients, 1,811 strains of pathogens were isolated from 1,686 cases (31.0%). several species including salmonella spp., escherichia coli serotype, vibrio parahaemolyticus, were isolated before 1978, and the incidence of pathogens was low (14.8%). for the 10-year period since 1979, the incidence markedly ... | 1991 | 1655921 |
clinical and serological studies in a series of 45 patients with guillain-barré syndrome. | we retrospectively reviewed the clinical files of 45 guillain-barré syndrome (gbs) patients admitted to our department between 1979 and 1989. the age distribution was bimodal with a first peak in young adults (20-40 years), and a second one between 60 to 70 years. seasonal distribution showed a late fall and a hivernal predominance. three patients experienced a second attack of gbs 2-9 years after the first one. thirty-one (69%) presented antecedent events, most often a respiratory tract infecti ... | 1991 | 1655983 |
hla-b35 and acute axonal polyneuropathy following campylobacter infection. | we performed hla typing for class i antigens on five japanese patients with acute polyneuropathy following campylobacter infection, all of whom showed axonopathy causing pure motor dysfunction associated with igg anti-gm1 antibodies. we demonstrated a statistically significant association between the disease and the hla-b35 antigen. | 1991 | 1656325 |
the in-vitro activity of temafloxacin compared with other antimicrobial agents. | temafloxacin, like other 4-quinolones, was highly active against most isolates of enterobacteriaceae. it was slightly less active than ciprofloxacin but approximately as active as ofloxacin and enoxacin. it was the most active of the quinolones against acinetobacter spp. and xanthomonas maltophilia but was slightly less active than ciprofloxacin against pseudomonas spp. temafloxacin was highly active against haemophilus influenzae, moraxella catarrhalis and neisseria gonorrhoeae and was the most ... | 1991 | 1657855 |
in vivo effects of sera from guillain-barré subgroups: an electrophysiological and histological study on rat nerves. | serum from 20 patients with guillain-barré syndrome (gbs), 10 healthy controls and 10 patients with recent cytomegalovirus, epstein-barr virus, or campylobacter jejuni/coli infections was injected into rat sciatic nerve. the 20 gbs patients consisted of 2 groups of 10 patients with different electrophysiological and clinical disease patterns. the main aim of the study was to investigate possible differences in humoral (auto)-immunity between these subgroups. we found no statistically significant ... | 1991 | 1658645 |
in vitro susceptibilities of campylobacter jejuni and campylobacter coli to azithromycin and erythromycin. | mics of azithromycin and erythromycin for 20 campylobacter coli and 20 campylobacter jejuni strains were determined. the results demonstrated that, for campylobacter species, all high-level erythromycin-resistant strains were also resistant to azithromycin and that azithromycin did not exhibit increased potency in comparison with that of erythromycin. | 1991 | 1659309 |
another case of atypical acute axonal polyneuropathy following campylobacter enteritis. | | 1991 | 1660574 |
[elisa for detection of anti-urease antibodies of campylobacter pylori and its application]. | the urease extracted from 6 strains of helicobacter pylori (hp) was used as antigen to detect sera anti-urease antibodies of hp in 136 patients with gastric diseases and 13 persons with normal gastric mucosa by enzyme-linked immunosorbent assay (elisa). the antigen proved to be pure and specific for elisa. for a titre of 450eu or over 450eu the test had a sensitivity of 91.96% and a specificity of 86.49%. the results of the elisa showed excellent correlation with microbiological detection of hp ... | 1991 | 1660762 |
spectrum of activity of azithromycin. | in recent years, a number of newer macrolides have been developed. one such antibiotic is azithromycin, which has a 15-membered ring structure and is classed as an azalide. the limitations of erythromycin and the discovery of pathogenic bacteria such as campylobacter, legionella and chlamydia species provide incentives to study the usefulness of newer antibiotics of this class. azithromycin has good activity against staphylococci, streptococci, moraxella catarrhalis and other rapidly growing pyo ... | 1991 | 1662624 |
in vitro activity of temafloxacin against gram-negative bacteria: an overview. | the in vitro activities of temafloxacin, ciprofloxacin, and ofloxacin against gram-negative bacteria are compared. the 90% minimal inhibitory concentrations (mic90s) of temafloxacin for respiratory pathogens such as haemophilus influenzae, moraxella catarrhalis, neisseria meningitidis, bordetella pertussis, and legionella pneumophila are less than or equal to 0.06 micrograms/ml. temafloxacin is also active against bacterial agents of sexually transmitted diseases, including neisseria gonorrhoeae ... | 1991 | 1662890 |
the prevalence of enteric pathogens in diarrhoeic thoroughbred foals in britain and ireland. | a survey of 77 normal and 326 diarrhoeic foals in britain and ireland from 1987 to 1989 revealed a significantly higher prevalence of group a rotaviruses and aeromonas hydrophila in diarrhoeic foals. the prevalence of cryptosporidia, potentially pathogenic escherichia coli, yersinia enterocolitica and clostridium perfringens was similar in normal or diarrhoeic foals. rotaviruses had a similar prevalence in all age groups of scouring foals up to three months of age, with an overall prevalence of ... | 1991 | 1663866 |
the in-vitro activity of tosufloxacin, a new fluorinated quinolone, compared with that of ciprofloxacin and temafloxacin. | tosufloxacin was highly active in vitro against most isolates of enterobacteriaceae. it was slightly less active than ciprofloxacin for most species but more active than temafloxacin. it was the most active agent against acinetobacter spp., xanthomonas maltophilia and some pseudomonas spp. but was slightly less active than ciprofloxacin against the fluorescent pseudomonads. tosufloxacin was highly active against strains of haemophilus influenzae, moraxella catarrhalis, neisseria gonorrhoeae and ... | 1991 | 1663930 |
[clinical-etiological correlations in 94 cases of acute infantile diarrhea]. | the authors consider the clinical and etiological correlations in 94 children (0-36 months) with acute diarrhoea, hospitalised in 1989. they compare epidemiological data (sex, age, seasonality), clinical data (fever, vomiting, distinctive features of diarrhoea, abdominal pain) and laboratory data with the main etiological agents isolated. | 1991 | 1666844 |
clostridium difficile typhlitis associated with cecal mucosal hyperplasia in syrian hamsters. | a sudden increase in mortality occurred in a closed breeding colony of syrian hamsters (mesocricetus auratus). the colony consisted of approximately 40 hamsters, 8 of which were affected. four adult males died suddenly. one pregnant female and one weanling died after having been observed as depressed for 1 day and 2 weeks respectively. one weanling and one adult male were euthanized. all affected hamsters had signs of diarrhea. at necropsy, hemorrhagic fluid-filled ceca were noted in five of eig ... | 1991 | 1667196 |
dual infection from cooked meats? | | 1991 | 1669791 |
sydney classification for gastritis. | | 1991 | 1670809 |
birds, milk, and campylobacter. | | 1991 | 1670812 |
birds, beaks, and campylobacter. | | 1991 | 1672198 |
campylobacter infections, guillain-barré syndrome, and parenteral gangliosides. | | 1991 | 1672406 |
[peptic ulcer and drug therapy]. | | 1991 | 1673699 |
[acute gastric mucosal lesion]. | | 1991 | 1673707 |
southern blot analysis of strain variation in campylobacter mucosalis. | a panel of three dna probes were derived at random from a genomic dna library of campylobacter mucosalis strain e8384-4. each probe hybridized specifically to c. mucosalis dna from bacteria fixed to nylon membranes. the probes did not hybridize to dna from other campylobacter species or to other bacteria even at 100-fold higher amounts. each probe hybridized to all of 24 isolates of c. mucosalis which had been collected over time from different geographic locations. southern blot analysis of sel ... | 1991 | 1673809 |
campylobacter upsaliensis enteritis associated with canine infections. | | 1991 | 1675356 |
isolation of novel microorganism from gastric biopsy specimen. | | 1991 | 1675386 |
[clinical course and treatment of duodenal ulcer in women]. | overall 260 women and 202 men were examined comparatively during duodenal ulcer exacerbation. 53.8% of the women demonstrated an atypical disease course. fibrogastroscopy is a method of early diagnosis of duodenal ulcer in women. as compared to men, the size and depth of ulcers in women was less. acid and peptic factor was also less pronounced. the four-week treatment with the use of the diet, antacids, gastrozepine and repair agents brought about ulcer healing in 82% of the women. the single an ... | 1991 | 1675494 |
sizing and mapping of the genome of campylobacter coli strain ua417r using pulsed-field gel electrophoresis. | agarose-immobilized chromosomal dna from the nalidixic-acid-resistant campylobacter coli strain ua417 and its streptomycin-resistant (strr) derivative, ua417r, were digested with the restriction enzymes sali (gtcgac) and smai (cccggg). the sizes of the resulting fragments were determined using pulsed-field gel electrophoresis. the two genomes showed similar restriction patterns of seven and 13 fragments for the two respective enzymes and the total genome size was determined to be approx. 1.7 mb. ... | 1991 | 1676383 |
campylobacter serology in patients with chinese paralytic syndrome. | | 1991 | 1677126 |
no signs of campylobacter jejuni/coli-related antibodies in patients with active ankylosing spondylitis. | twenty-two patients with active ankylosing spondylitis were investigated to assess the levels of specific serum igg, iga and igm titres against campylobacter jejuni/coli before and during treatment with sulfasalazine. an enzyme-linked immunosorbent assay was used, and the results were compared with the antibody levels in 300 healthy blood donors. three patients had elevated levels of serum anti-campylobacter-iga before treatment, and a two-fold decrease in the antibody titre was observed during ... | 1991 | 1677583 |
management of campylobacter enteritis in hospitalized patients. | | 1991 | 1677661 |
birds, milk, and campylobacter. | | 1991 | 1678050 |
pancreatitis associated with salmonella enteritis. | | 1991 | 1678822 |
campylobacter spp isolated from dog faeces. | | 1991 | 1682769 |
restriction fragment length polymorphisms in the ribosomal genes for species identification and subtyping of aerotolerant campylobacter species. | whole-cell chromosomal digests of 84 strains of aerotolerant campylobacter (ac) were examined by using pvuii restriction fragment length polymorphisms of rrna genes followed by hybridization with escherichia coli 16s and 23s rrna (ribotyping). the ac strains belonged to campylobacter cryaerophila (n = 13) and a newly defined species, "c. butzleri" (n = 64). strains of c. cryaerophila belonged to two hybridization groups: dna group 1a (including the type strain of c. cryaerophila) and dna group 1 ... | 1991 | 1684797 |
a comparison of the in-vitro activity of clarithromycin, a new macrolide antibiotic, with erythromycin and other oral agents. | on the basis of minimum inhibitory concentrations clarithromycin (6-o-methylerythromycin), a new macrolide, was found to be slightly more active than erythromycin against staphylococcus aureus, enterococci. moraxella catarrhalis, gardnerella vaginalis, bacteroides fragilis (sensu stricto) and b. ureolyticus and slightly less active against coagulase-negative staphylococci, alpha- and beta-haemolytic streptococci, haemophilus influenzae, campylobacter coli/jejuni and the b. melaninogenicus/oralis ... | 1991 | 1684981 |
acridine orange fluorescence, campylobacter pylori, and chronic gastritis. | endoscopic gastric biopsy specimens from 230 consecutive patients in a north liverpool district were histologically studied by routine light microscopy and ultraviolet fluorescence after acridine orange staining. eighty patients with chronic gastritis were further studied with regard to type of gastritis and its activity, presence of campylobacter pylori (cp), and degree of colonization of the gastric mucosa. miscellaneous gastritis, gastric ulcers, erosions, neoplasms, and histologically normal ... | 1990 | 1689506 |
a five minute stain for campylobacter in tissue. | | 1990 | 1690759 |
[a method of isolating campylobacter pylori from the gastrointestinal mucosa]. | | 1990 | 1692368 |
[diagnosis of campylobacter pylori in chronic gastritis, gastric ulcer and duodenal ulcer (review of the literature)]. | | 1990 | 1695273 |
[diagnosis of campylobacter pylori based on the level of urea in the gastric juice]. | comparison of urea levels in 3 portions of gastric juice, obtained on an empty stomach, basal, and stimulated, from 28 patients, c. pylori carriers, and 20 patients without c. pylori carriership, has shown that the presence of this bacterium in the gastric mucosa is related not so much with a decrease in the absolute level of urea in the portion collected on an empty stomach but mostly with changes in this parameter in three portions of gastric juice collected in succession. decreased urea level ... | 1990 | 1695282 |
comparison of culture and histology for the identification of helicobacter pylori in endoscopic biopsies. | the aim of the study was to compare culture with microscopic examination of haematoxylin and eosin stained tissue sections in the identification of helicobacter pylori in gastric antral mucosa. duplicate gastric antral biopsies from 89 patients were cultured and examined histologically for h pylori. h pylori was cultured in 46 cases (51%) and identified histologically in 63 (71%). results from the two tests concurred in 63 cases (71%). the presence of h pylori was strongly correlated with histol ... | 1990 | 1697451 |
antigenic differences among campylobacter fetus s-layer proteins. | sodium dodecyl sulfate-polyacrylamide gel electrophoresis of s-layer proteins extracted from campylobacter fetus strains by using acid glycine buffer showed that the predominant s-layer proteins of different strains had subunit molecular weights in the range of 90,000 to 140,000. electron microscopy revealed oblique s-layer lattices with a spacing of approximately 5.6 nm (gamma = 75 degrees) on wild-type strains vc1, vc119, vc202, and vc203. three variants of c. fetus vc119 producing a predomina ... | 1990 | 1697574 |
use of dna restriction endonuclease digest and ribosomal rna gene probe patterns to fingerprint helicobacter pylori and helicobacter mustelae isolated from human and animal hosts. | variation amongst strains of helicobacter pylori and helicobacter mustelae was examined by dna restriction endonuclease digestion and rrna gene patterns generated using a non-radioactive probe. chromosomal dna was extracted from 30 cultures of h. pylori from human, rhesus monkey and pig gastric mucosa, and from three h. mustelae isolates from ferret gastric mucosa. dna fingerprinting with hae iii and hind iii showed h. mustelae was relatively homogeneous but revealed genomic heterogeneity within ... | 1990 | 1698260 |
dna restriction digest and ribosomal rna gene patterns of campylobacter jejuni: a comparison with bio-, sero-, and bacteriophage-types of united kingdom outbreak strains. | dna restriction endonuclease (hae iii and hind iii) total digest and 16s and 23s ribosomal (r)rna gene patterns (ribopatterns) were determined for 18 isolates of campylobacter jejuni from three separate outbreaks of diarrhoea in the north of england. strains were also characterized by biotyping, serotyping and phage typing. comparisons of the dna patterns by visual and numerical methods revealed five distinct strain groupings with clear differences between isolates from different outbreaks as we ... | 1990 | 1698647 |
detection of antibodies to helicobacter pylori cell surface antigens. | serum igg antibodies of helicobacter pylori were detected in single-dilution elisa using glycine extracted material. among 148 endoscopy patients 59% displayed antibodies; as expected, a higher occurrence (90%) was found in patients with positive gastric culture for h. pylori than in culture negative patients (37%). among 68 blood donors the frequency of h. pylori antibodies was 28%. in 73 children less than 15 years of age examined for unrelated disorders the occurrence was 4%. by immunoblottin ... | 1990 | 1699265 |
identification, characterization, and spatial localization of two flagellin species in helicobacter pylori flagella. | flagellar filaments were isolated from helicobacter pylori by shearing, and flagellar proteins were further purified by a variety of techniques, including cscl density gradient ultracentrifugation, ph 2.0 acid disassociation-neutral ph reassociation, and differential ultracentrifugation followed by molecular sieving with a sephacryl s-500 column or mono q anion-exchange column, and purified to homogeneity by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transfer to an ... | 1991 | 1704004 |
[the determination of c. pylori in patients with gastroduodenal diseases]. | a total of 46 gastric mucosa biopsy specimens obtained during surgery and 90 gastric juice samples from patients with gastroduodenal diseases were examined. campylobacter-like microorganisms were detected in 84.6 percent of biopsy specimens and in 73.9 percent of gastric juice samples (in gram-stained smears). urease activity was detected in 75.9 and 13.3 percent, respectively. c. pylori growth in solid nutrient medium in microaerophilic conditions was observed in 44.2 and 5.7 percent of the exa ... | 1990 | 1704468 |
revision of campylobacter, helicobacter, and wolinella taxonomy: emendation of generic descriptions and proposal of arcobacter gen. nov. | hybridization experiments were carried out between dnas from more than 70 strains of campylobacter spp. and related taxa and either 3h-labeled 23s rrnas from reference strains belonging to campylobacter fetus, campylobacter concisus, campylobacter sputorum, campylobacter coli, and campylobacter nitrofigilis, an unnamed campylobacter sp. strain, and a wolinella succinogenes strain or 3h- or 14c-labeled 23s rrnas from 13 gram-negative reference strains. an immunotyping analysis of 130 antigens ver ... | 1991 | 1704793 |
localization of immunogenic regions on the flagellin proteins of campylobacter jejuni 81116. | the purpose of this study was to localize antigenic regions on the flagellin protein of campylobacter jejuni 81116. this strain has two flagellin genes, flaa and flab, which are 95% identical; only flaa seems to be expressed in motile c. jejuni 81116 cells. fragments of flaa and flab were cloned in the bacterial expression vector pex, and the expression products were incubated with flagellin-specific antibodies. monoclonal antibodies to broadly cross-reactive epitopes recognized fragments that a ... | 1991 | 1705240 |
dna-dna hybridization and analysis of restriction endonuclease and rrna gene patterns of atypical (catalase-weak/negative) campylobacter jejuni from paediatric blood and faecal cultures. | sixteen strains of atypical (catalase-weak or negative), hippurate-hydrolysing campylobacters from paediatric blood and faecal cultures were identified by dna-dna slot hybridization. all were closely related (greater than or equal to 67%) to campylobacter jejuni and representative strains had g + c contents of 30 +/- 1 mol%. numerical analysis of chromosomal dna haeiii digest patterns revealed two clusters of strains at the 55%s level corresponding to c. jejuni subsp. jejuni and c. jejuni subsp. ... | 1991 | 1707870 |
physicochemical characteristics and flora of diarrhoeal and recovery faeces in children with acute gastro-enteritis in kenya. | physiochemical characteristics and flora of diarrhoeal and recovery faeces were investigated in 14 kenyan children with acute gastro-enteritis. causative micro-organisms were shigella, campylobacter, enterotoxigenic escherichia coli, rotavirus and unknown in 6, 2, 1, 2 and 3 patients, respectively. the mean values of the ph of the diarrhoeal specimens were significantly higher than those of the recovery specimens. large amounts of acetic acid and many other kinds of fatty acids were detected in ... | 1990 | 1708960 |
cytodiagnosis of campylobacter pylori in papanicolaou-stained imprints of gastric biopsy specimens. | the use of papanicolaou-stained touch preparations of gastric antral biopsies for the identification of campylobacter pylori was examined using specimens obtained from 63 consecutive patients with endoscopic evidence of antral gastritis, with the results compared to routine histologic examination and warthin-starry silver staining. organisms were readily identifiable in the papanicolaou-stained imprints of the gastric mucus. the sensitivity in detecting organisms was 92.5% for the warthin-starry ... | 1991 | 1709322 |
atypical structure of the 23s ribosomal rna molecule in certain oral bacteria. | ribosomal rna (rrna) isolated from wolinella recta and seven related bacteria was examined by agarose gel electrophoresis. the 23s rrna molecule could not be detected in w. recta, wolinella curva, bacteroides gracilis, or bacteroides ureolyticus. in place of the 23s molecule, there were three smaller molecules of approximately 1700, 650, and 600 bases designated 23s alpha, 23s beta, and 23s delta, respectively. an intact 23s rrna molecule could be isolated from wolinella succinogenes, campylobac ... | 1991 | 1710629 |
[intestinal microbial pathology in aids. a clinical case series]. | microbial isolates from 60 diarrheic aids patients hospitalized to the infectious disease division of careggi hospital (florence) are described. clinical, microbiological and diagnostic features of each case are discussed with emphasis to some rare or underestimated entities in europe: campylobacter laridis bacteremia, whipple-like disease by atypical mycobacteria, schistosoma mansoni proctocolitis. results regarding newly aids-related microorganisms are also stressed. | 1991 | 1710813 |
[the specificity of campylobacter jejuni antigens in immunoenzyme analysis]. | the surface antigen has been obtained from c. jejuni culture and its immunochemical analysis has been carried out by the methods of immunoelectrophoresis, double diffusion with homologous and heterologous rabbit sera. the protein profile of this antigen has been studied by means of vertical electrophoresis in polyacrylamide gel with sodium dodecyl sulfate, and the molecular weight of the protein components of the antigen has been determined. the presence of anti-campylobacter antibodies has been ... | 1990 | 1711263 |
[helicobacter pylori--a pathogen with many faces]. | | 1991 | 1714173 |
[virulence factors of helicobacter pylori and pathomechanisms of mucosal damage]. | | 1991 | 1714174 |
[effect of helicobacter pylori on stomach physiology]. | | 1991 | 1714175 |
[helicobacter pylori infection: a facilitator of stomach cancer?]. | | 1991 | 1714176 |
[epidemiology and diagnosis of helicobacter pylori infection]. | | 1991 | 1714177 |
[helicobacter pylori infection, gastritis and non-ulcer dyspepsia--is there a connection?]. | | 1991 | 1714178 |
[methods of isolating and identifying campylobacter pylori (review of the literature)]. | | 1991 | 1714997 |
cytotoxic effects of children's faeces: relation to diarrhoea due to clostridium difficile and other enteric pathogens. | cytotoxicity of faecal extracts was demonstrated in 47 of 88 children (54%) referred for microbiological investigation of stools. cytotoxic clostridium difficile and vertotoxigenic escherichia coli (vtec) were the pathogens identified most commonly but cytotoxicity was also found in association with campylobacter jejuni, salmonella spp, shigella sonnei, giardia lamblia, rotavirus, adenovirus and poliovirus type 1 which had been acquired by oral immunization. in two patients, one of whom had cyst ... | 1991 | 1715139 |
rapid diagnosis of campylobacter pylori infection by urea test paper. | antral biopsy specimens from 106 patients were examined by culture, gram stain and silver stain for campylobacter pylori. biopsies were also examined by a urea test paper test (utpt). of 106 patients studied c. pylori was detected in 68 (64.2%) by gram stain, silver stain and culture. the utpt was positive in 63 (59.4%) specimens. five had false negative results using the utpt with no false positive subjects. thus, utpt has a sensitivity of 92.6% (63/68) and a specificity of 100%. of the 63 spec ... | 1990 | 1717032 |
evaluation of a simplified procedure for serotyping campylobacter jejuni and campylobacter coli which is based on the o antigen. | a simplified procedure for serotyping campylobacter jejuni and campylobacter coli on the basis of thermostable antigens was developed and tested for its applicability as a routine typing method. the assay involves the sensitization of erythrocytes with an antigenic extract and performance of a slide agglutination assay with specific antisera. in order to simplify the typing system to a greater extent, the standard typing antisera were pooled into nine groups for c. jejuni and four groups for c. ... | 1991 | 1719020 |
detection of helicobacter pylori in stomach tissue by use of a monoclonal antibody. | monoclonal antibodies were produced against an acid glycine extract of helicobacter pylori atcc 43504t. one of these appeared to be specific for h. pylori; it recognized all h. pylori isolates by an indirect immunofluorescence assay (iif) but it did not cross-react with the other strains tested, including different species of the genera helicobacter, campylobacter, and wolinella. different strains of members of the families enterobacteriaceae and pseudomonadaceae or other gram-negative bacteria ... | 1991 | 1721923 |
[the diagnosis of campylobacteriosis. 1]. | | 1991 | 1721962 |
a dna sequence upstream of the tet(o) gene is required for full expression of tetracycline resistance. | the dna sequences upstream of the tet(o) and tet(m) open reading frames (orfs) (ca. 300 bp) were found to share a higher degree of homology than those of the tet(o) and tet(m) orfs themselves. a transcription initiation site for tet(o) was located by primer extension analysis. campylobacter coli was found to use a promoter sequence different from that used by escherichia coli. the sequence upstream of tet(o) was shown to be required in cis for high-level resistance to tetracycline. | 1991 | 1722078 |
oligodeoxynucleotide probes for campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequences. | deoxyoligonucleotide probes were constructed for the identification of campylobacter fetus and campylobacter hyointestinalis based on 16s rrna sequence data. probes were targeted to hypervariable regions of 16s rrna. specificity of oligonucleotide probes was tested in a colony blot assay with type strains of 15 campylobacter and arcobacter species as well as in a slot blot format using genomic dna extracted from field strains of c. fetus and c. hyointestinalis. two oligonucleotides were construc ... | 1991 | 1723076 |
[campylobacter infections]. | | 1991 | 1724163 |
[morphological and morphometric characteristics of the large-intestinal mucosa in campylobacter infections]. | twenty-two biopsy specimens of colon mucosa were obtained from 16 patients with gastrointestinal campylobacteriosis histologically, histochemically and morphometrically. at the height of the disease colon mucosa of these patients showed a morphological picture of acute hemorrhagic and erosive--hemorrhagic colitis. when compared with such for other intestinal infections (shigellosis, salmonellosis, rotaviral gastroenteritis), the morphological features appeared to vary permitting a differential d ... | 1991 | 1724708 |
[utility of the l-alanine-aminopeptidase test for differentiating the cell wall structure of bacteria]. | evaluation of a detection test for l-alanine-aminopeptidase enzyme (bactident aminopeptidase) for determining the structure of bacterial cell wall. in a total of 246 clinical isolates of aerobic, microaerophilic and anaerobic bacteria, we detect the presence or absence of l-alanine-aminopeptidase using commercial kits (bactident aminopeptidase, merck diagnostica). we also identify and further classified the 246 strains. in nearly all gram-negative bacteria l-alanine-aminopeptidase was found, wit ... | 1991 | 1726575 |
specific serotype of campylobacter jejuni associated with guillain-barré syndrome. | | 1992 | 1727892 |
evolutionary relationships among sulfur- and iron-oxidizing eubacteria. | some 37 reverse transcriptase, partial 16s rrna sequences from sulfur- and/or iron-oxidizing eubacteria, including sequences from species of the genera thiobacillus, thiothrix, thiomicrospira, acidophilium, "leptospirillum," thiovulum, and chlorobium, have been determined. in addition, 16s sequences from a number of unnamed sulfur- and/or iron-oxidizing bacteria from hydrothermal vent sites, from invertebrate-bacterial endosymbioses, and from various mineral recovery operations also have been de ... | 1992 | 1729214 |
seasonal variation in etiology of travelers' diarrhea. finnish-moroccan study group. | the etiology of travelers' diarrhea was studied in 579 adult finnish tourists participating in two packaged tours to morocco in the winter (n = 233) and fall (n = 346) of 1989. a research team accompanied the travelers, and a laboratory for enteric pathogens was established in agadir. at least one pathogen was found in 62% of the 60 diarrhea cases in winter and in 58% of the 111 diarrhea cases in fall. multiple pathogens were found less often in winter (8%) than in fall (21%, p less than .05). c ... | 1992 | 1730905 |
lysyl-trna synthetase gene of campylobacter jejuni. | we report the cloning and complete nucleotide sequence of the campylobacter jejuni lysyl-trna synthetase gene (lyss). the c. jejuni lyss gene sequence shows high homology to the two escherichia coli lysyl-trna synthetase genes, lyss and lysu. the campylobacter lysyl-trna synthetase protein (lysrs) shows 47.9 and 46.6% sequence identity to the e. coli enzymes encoded by the lyss and lysu genes, respectively. the lysrs encoded by the c. jejuni gene is a polypeptide of 501 amino acids with a deduce ... | 1992 | 1732205 |
an outbreak of bacteremic campylobacter jejuni infection. | during september 1980, an outbreak of bacteremic campylobacter jejuni infection occurred in metropolitan los angeles. the outbreak was recognized when blood cultures obtained from 11 previously healthy persons with acute febrile illnesses (characterized in over 80% by fever, diarrhea, and headaches) were positive for c. jejuni. all recovered after an illness that lasted a mean of 8 days. a surveillance system failed to reveal a concomitant outbreak of gastroenteritis. isolates had identical bioc ... | 1992 | 1734239 |
reattachment of surface array proteins to campylobacter fetus cells. | campylobacter fetus strains may be of serotype a or b, a property associated with lipopolysaccharide (lps) structure. wild-type c. fetus strains contain surface array proteins (s-layer proteins) that may be extracted in water and that are critical for virulence. to explore the relationship of s-layer proteins to other surface components, we reattached s-layer proteins onto s- template cells generated by spontaneous mutation or by serial extractions of s+ cells with water. reattachment occurred i ... | 1992 | 1735716 |
comparison of two simple microaerobic atmospheres for cultivation of thermophilic campylobacters. | a comparison of a modification of the spirit burn method and one based on the fortner principle was carried out on 3474 faecal samples from patients with diarrhoea. of the 96 isolates of campylobacter, 47 showed equal abundance of growth, regardless of the method used. by using the spirit burn method, however, growth of 33 isolates was significantly increased, and 18 out of those 33 isolates grew only in the spirit burn method atmosphere. the spirit burn method is more effective than the one bas ... | 1992 | 1740527 |
[development of genetic and molecular approaches for the diagnosis and study of the pathogenicity of helicobacter pylori, agent of gastric inflammatory diseases]. | helicobacter pylori (h. pylori) is a small gram-negative bacillus, recently discovered, found in the stomach of patients with active chronic gastritis and duodenal ulcers. production of a potent urease has been described as a trait common to all h. pylori so far isolated. to clarify the role of urease in the pathogenic process, as well as to engineer genetic tools useful for the diagnosis of h. pylori, we cloned the genes responsible for urease activity. a genomic library was constructed in esch ... | 1991 | 1742619 |
can isolation of aeromonas hydrophila from human feces have any clinical significance? | a total of 27,480 stool specimens from 15,548 patients with gastroenteritis were analyzed for bacterial enteropathogens during a 4-year period between 1986-89 at a major referral center in saudi arabia. bacterial pathogens were isolated from 1,152 patients, salmonella being the most frequent, followed by campylobacter, shigella, and aeromonas hydrophila. the latter bacterium was found in 58 patients; seven of them were associated with other enteric pathogens, but a. hydrophila was the only organ ... | 1991 | 1744389 |
etiology and management of diarrhoeal diseases in karachi. | the incidence of diarrhoeal disease are very high in our population and bacterial etiology amounts to 43.55% of all cases. pathogen such as aeromonas hydrophila, plesiomonas shigelloides, yersinia enterocolitica and campylobacter are also present in significant numbers, they make up 50% of bacterial causes. all enteric pathogens can be easily isolated on commonly used media and could be precisely identified by simple biochemical tests. | 1991 | 1744967 |
evaluation of various laboratory techniques to diagnose helicobacter pylori in patients with upper gastro-intestinal tract symptoms. | helicobacter (campylobacter) pylori is strongly associated with type b gastritis. the detection of h. pylori, which entails histological examination and culture of gastric biopsy specimens, takes several days. there has been much interest in developing more rapid tests, including non-invasive ones. using histology and/or culture as the 'gold standard', several methods to detect h. pylori were compared and evaluated. the organism was detected in 84 of 100 consecutive patients attending the gastro ... | 1991 | 1745946 |
[isolation of yersinia, campylobacter, plesiomonas and aeromonas from environmental water and fresh water fishes]. | yersinia, campylobacter, plesiomonas and aeromonas are known causative agents in waterborne diseases. for about 10 years, outbreak of diarrhea has been observed, especially among children, in the mountain areas of okayama. y. pseudotuberculosis recently isolated from non-chlorinated drinking water sources such as mountain streams and wells has been suspected to be the causative bacteria of the disease. attempts were made to isolate yersinia and campylobacter from water samples from rivers in rur ... | 1991 | 1747520 |
[analysis of outer membrane proteins from eight strains of campylobacter in sds-page]. | the outer membrane proteins (omps) from 8 strains of campylobacter, including 5 reference strains and 3 local strains, were isolated and examined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). the results showed that the population distributions of molecular weight of omps from different species and sources were alike based on the rank sum test analysis. the number of bands of major omp was around 2-3, the molecular weight of which varied from 33-68 kd. moreover, three ... | 1991 | 1748411 |
surveillance of patients attending a rural diarrhoea treatment centre in bangladesh. | in may 1983, a surveillance system was set up at a rural diarrhoea treatment centre of the international centre for diarrhoeal disease research, bangladesh (icddr,b) to study a 20% systematic sample of all admissions. between may 1983 and april 1984, 2,635 patients were studied. a recognized enteric pathogen was detected in 69% of the patients screened, 15% of whom had a mixed infection. vibrio cholerae 0: 1 was the most common enteropathogen detected (39%), followed by enterotoxigenic escherich ... | 1991 | 1750109 |
a note on the comparative efficacy of three selective media for isolation of campylobacter species from environmental samples. | the efficacy of three selective broths and agars, i.e. preston-, mccd- and car medium, were compared to each other in various combinations. twelve campylobacter coli and 9 campylobacter jejuni strains that had been isolated from sewage were used as test strains. to evaluate the applicability for highly contaminated environmental samples 15 sewage samples were examined, subsequently. significant differences between the media could not be ascertained. the application of preston broth/agar or car b ... | 1991 | 1750964 |
evaluation of media for primary isolation of campylobacter jejuni from faecal samples from children and animals. | different media were used for primary isolation of campylobacter. butzler & preston medium was found to be more selective compared to skirows & blaserwang. | 1991 | 1752634 |
fluoroquinolone resistance in campylobacter jejuni and campylobacter coli isolated from human faeces in switzerland. | | 1991 | 1753149 |
dental plaque: a permanent reservoir of helicobacter pylori? | the aim of the study was to observe the relationship between the two reservoirs of helicobacter pylori--that is, dental plaque and the stomach. with the campylobacter-like organism (clo) test, h. pylori was detected in dental plaque and in gastric antral and body mucosa in 98%, 67% and 70%, respectively, of 43 consecutive patients with dyspepsia. the rapidity of the clo test indicates that the density of h. pylori is heaviest in dental plaque, less in the antrum, and least in the body mucosa of ... | 1991 | 1754858 |
[bismuth revisited in helicobacter pylori gastro-duodenal infection]. | the various hypothesis for the explantation of reversible bismuth encephalopathy are reviewed and discussed. today, this side effect appears to be devoid of mystery, due to uncontrolled and inhibited ingestion of bismuth salts which increased the emergence of genetic and physiopathologic risk factors and brought about the cases of neurotoxicity. bismuth therapy has shown major efficacy in the treatment of gastroduodenal ulcers. colloidal bismuth subcitrate is as effective as the h2-receptor anta ... | 1991 | 1754987 |
capillary gas chromatography of cellular carbohydrates as a means for the differentiation of fastidious, slow-growing or anaerobic gram-negative bacteria--a review. | the phenotypic differentiation of microaerophilic or anaerobic gram-negative bacteria of human origin is traditionally based on the analysis of metabolic characteristics. due to outstanding growth requirements of many kinds of parasitic bacteria, e.g. of the facultatively and strictly anaerobic members of the human periodontal flora, conventional biochemical tests are time-consuming and sometimes lead to doubtful results. commercial diagnostic test kits often produce misidentifications because o ... | 1991 | 1755919 |
potential sources of campylobacter species in the homes of farmworkers in zimbabwe. | chicken faeces, houseflies, household stored drinking water, cooked or prepared foods and handwashings of people of all age groups and hands of children under 5 years old were examined as potential sources and transmission routes of campylobacter species in a farmworker community. campylobacter species were found to be common in chicken faeces collected from the homesteads of the farmworkers but were rarely isolated from houseflies or household stored drinking water. they were not isolated from ... | 1991 | 1758009 |
[the pathogenesis of stomach and duodenal involvement in chronic opisthorchiasis. 1. the urease test]. | the study using the urease test on mucous biopsies from the antral gastric part and from the duodenum of patients with chronic opisthorchiasis with endoscopic evidence of antral gastritis and gastroduodenitis, and from noninvaded patients with gastritis and duodenitis, some of them with the gastric or duodenal ulcers showed that the test was positive. the test was negative in both groups of patients when the mucosa of the gastric body was examined as well as in those without gastroduodenal patho ... | 1991 | 1758356 |
[gastritis and campylobacter pylori. 85 cases]. | | 1991 | 1759317 |