| expression of the epstein-barr virus encoded latent membrane protein in tumor cells of hodgkin's disease occurring in childhood. | paraffin sections from 21 cases of hodgkin's disease (hd), 28 cases of non-hodgkin's lymphomas (nhl) and 34 cases of non-specific reactive lymphadenitides occurring in childhood were examined for the presence of the epstein-barr virus (ebv)-encoded latent membrane protein (lmp) using a double layer immunohistochemical method. lmp was detected in 12/21 (57%) cases of hd but not in nhl or reactive lymph nodes. lmp reactivity was restricted to reed-sternberg and hodgkin's (hrs) cells in 4 of 9 (45% ... | 1992 | 1365766 |
| a human-human hybridoma secreting anti-pseudomonas aeruginosa exotoxin-a monoclonal antibody with highly potent neutralizing activity. | a hybridoma secreting human monoclonal antibody (mab) against pseudomonas aeruginosa exotoxin a (pea) was constructed by fusing epstein-barr virus-transformed peripheral blood lymphocytes with human b lymphoblastoid cell line taw-925. the human-human hybridoma stably produced human igg2 mab at the rate of 0.4-0.5 microgram/ml per 10(6) cells per day for more than six months, and the mab was capable of neutralizing the in vitro cytotoxic and in vivo lethal effects of pea with approximately 100- a ... | 1990 | 1369271 |
| subclinical epstein-barr virus infection of both the male and female genital tract--indication for sexual transmission. | epstein-barr virus (ebv) can infect b lymphocytes as well as epithelial cells of the oral cavity. recently, infection of epithelial cells of the inflamed uterine cervix has been demonstrated, and ebv-dna has been detected in urethral discharge of men suffering from genital infection. we investigated whether ebv can be found in the genital tract of both sexes independently from inflammatory disease states. genital specimens of men and women of a sexually transmitted diseases outpatient clinic aft ... | 1992 | 1314867 |
| factor(s) required by ebv transformed lymphocytes to grow under limiting dilution conditions. | il-6 was demonstrated to promote growth of ebv transformed lymphocytes. however il-6 was ineffective at promoting growth of ebv transformed lymphocytes cultured at the single cell level under limiting dilution conditions. on the contrary, hecs, which is known to contain il-6, supported very efficiently the growth of 1-2 ebv transformed cells. when il-6 was removed from hecs, by using specific antibodies, no reduction in hecs activity was observed, indicating that probably more than one growth fa ... | 1991 | 1369369 |
| possible anti-tumor promoting properties of marine algae and in vivo activity of wakame seaweed extract. | | 1992 | 1369377 |
| multilocus dna fingerprint analysis of cell banks: stability studies and culture identification in human b-lymphoblastoid and mammalian cell lines. | the technique of multilocus dna fingerprinting has great potential for the authentication of animal cell cultures and in identification of cross-contamination. the alec jeffreys probes 33.6 and 33.15 were used as multilocus probes to demonstrate the consistent dna fingerprint profiles in human peripheral blood and its derivative epstein-barr virus (ebv) transformed b-lymphoblastoid cultures maintained by repeated subculture for six months. however, fingerprint analysis of ebv transformed culture ... | 1992 | 1369387 |
| epstein-barr virus-associated gastric adenocarcinoma. | the epstein-barr virus (ebv) has been detected in certain types of lymphoma and some epithelial neoplasms including nasopharyngeal lymphoepithelioma, and rare lymphoepithelioma-like carcinomas occurring in a variety of organs including, most recently, the stomach. the authors investigated the possibility that ebv may be present not only in the rare gastric cancers that resemble nasopharyngeal lymphoepithelioma, but also in typical gastric adenocarcinoma. ebv sequences were detected in 22 of 138 ... | 1992 | 1314023 |
| [severe leukopenia and thrombocytopenia and transient acquired hypogammaglobulinemia in a patient with infectious mononucleosis]. | a 30-year-old male with infectious mononucleosis by the epstein-barr virus who presented severe neutropenia and thrombocytopenia and type igg acquired transitory hypogammaglobulinemia as complications during the acute period of the disease is presented. although the etiopathogenesis of these phenomena is usually associated with an autoimmune basis, the antiplatelet and antileukocyte antibodies were negative. a bibliographic revision of the hematologic alterations of this disease was carried out ... | 1992 | 1313937 |
| control of human b cell tumor growth in severe combined immunodeficiency mice by monoclonal anti-b cell antibodies. | severe combined immunodeficiency (scid) mice develop ebv (+)b cell tumors after infusion of ebv(+)b cells or of b cells and ebv. in this study, scid mice were infused with b cell lines derived from three patients who developed a b lymphocyte proliferative disorder after bone marrow or organ transplantation. intraperitoneal injection of 5 x 10(6) b cells induced tumor growth in all mice, leading to death within 60 d. human b cells were identified in spleen and bone marrow by means of immunofluore ... | 1992 | 1326002 |
| expression of a family of complementary-strand transcripts in epstein-barr virus-infected cells. | a major family of polyadenylylated cytoplasmic transcripts are expressed from the bamhi a-i region of the epstein-barr virus genome, off the strand complementary to that encoding several functions associated with viral replication and the lytic cycle, including the dna polymerase (balf-5). these complementary-strand transcripts (the main one is about 4.8 kilobases long), expressed in all cell types associated with epstein-barr virus, are present at high levels in nasopharyngeal carcinoma tumors. ... | 1992 | 1325642 |
| t-cell lymphoma and the virus-associated hemophagocytic syndrome. | a 20-month-old girl had a disorder that by both clinical and histologic criteria resembled the virus-associated hemophagocytic syndrome in the setting of epstein-barr virus infection. subsequent investigation revealed histologic evidence of disseminated t-cell lymphoma. dna hybridization studies displayed a monoclonal t-cell receptor beta chain rearrangement, in the absence of clonal immunoglobulin gene rearrangement, and a single band in the analysis for the fused termini of the epstein-barr vi ... | 1992 | 1372145 |
| increase of peripheral b lymphocytes committed to the production of monoreactive and high affinity antibodies to htlv-1 in patients with ham/tsp. | we quantitated the frequency of b lymphocytes capable of producing antibodies to htlv-1 in the peripheral blood from patients with ham/tsp, non-ham/tsp htlv-1 carriers and seronegative healthy subjects. epstein-barr virus (ebv) was used as a polyclonal activator of b lymphocytes in a limiting dilution condition. we found that b lymphocytes committed to the production of monoreactive-igg and -iga antibodies to recombinant htlv-1 (gag + env) hybrid protein were significantly increased in a number ... | 1992 | 1372329 |
| growth- and differentiation-associated expression of bcl-2 in b-chronic lymphocytic leukemia cells. | the bcl-2 gene is translocated into the ig loci in about 80% of human follicular lymphomas and in 10% of b-type chronic lymphocytic leukemias (b-cll), resulting in a high level of expression. we have compared the expression of bcl-2 transcripts and protein in b-cll cells in their normal equivalent cd5+ b cells and in normal b-cell populations representative of different in vivo and in vitro stages of activation and proliferation. we report here that bcl-2 was expressed in 11 of 11 cases of cd5+ ... | 1992 | 1375120 |
| relapsing acute disseminated encephalomyelitis associated with chronic epstein-barr virus infection: mri findings. | a 25-year-old women had a fever, left cervical lymphadenopathy, neurological symptoms and signs, csf pleocytosis and persistent high serum antibodies to the epstein-barr virus (ebv); she had a recurrence 1 year later. she was thought to have relapsing acute disseminated encephalomyelitis associated with chronic ebv infection. mri revealed abnormalities, mainly in the right basal ganglia and left midbrain. at the time of the recurrence, further abnormalities appeared in the opposite basal ganglia ... | 1992 | 1326729 |
| biochemical analysis of hla-dr antigens using one-dimensional isoelectric focusing gel electrophoresis. | | 1992 | 1326792 |
| specificity and idiotope expression of igm produced by cd5+ and cd5- cord blood b-cell clones. | epstein-barr virus (ebv)-immortalized monoclonal b-cell lines were established from cd5+ and cd5- cord-blood b cells. igm from many of both cd5+ and cd5- clones reacted with igg-fc, ssdna, and a variety of other autoantigens. more cd5+ b cells that used light chains of the kappa isotype reacted with igg-fc and ssdna than kappa-bearing cd5- b cells. because many of the clones reacted with igg-fc, they were analyzed for the expression of cross-reactive idiotypes (cri) associated with rheumatoid fa ... | 1992 | 1376073 |
| epstein-barr virus in lymphoma. protagonist or passenger? | | 1992 | 1326890 |
| fireside conference 16. nasopharyngeal carcinoma. | | 1992 | 1327018 |
| molecular basis for the association between hla dr4 and rheumatoid arthritis. from the shared epitope hypothesis to a peptidic model of rheumatoid arthritis. | susceptibility to rheumatoid arthritis (ra) maps to residues qkraa/qrraa in the third hypervariable region of the hla dr beta 1 chain. peptides from the same area of mhc class ii molecules are able to modulate the t-cell repertoire by deleting self-reactive t-cells. the epstein barr virus glycoprotein gp110 and the dna j heat-shock protein from e. coli mimic the third hypervariable region of hla-dw4dr beta 1. thus, the same area of hla dr beta 1 carries susceptibility to ra, modulates the t-cell ... | 1992 | 1378777 |
| identification of new epitopes recognized by human monoclonal antibodies with neutralizing and antibody-dependent cellular cytotoxicity activities specific for human t cell leukemia virus type 1. | we have generated a number of ebv-transformed b cell lines producing human mab against human t cell leukemia virus type 1 (htlv-1) from the peripheral blood b lymphocytes obtained from patients with htlv-1-associated myelopathy/tropical spastic paraparesis. various synthetic peptides corresponding to antigenic regions of htlv-1 gag and env proteins were used for the screening of antibodies in elisa. in our study, four igg mab to the gag p19 amino acids 100 to 130, and 5 igg mab to the env p46 am ... | 1992 | 1378869 |
| antibodies against epstein-barr nuclear antigen (ebna) in multiple sclerosis csf, and two pentapeptide sequence identities between ebna and myelin basic protein. | the epstein-barr virus (ebv) causes infectious mononucleosis and is linked to several disparate malignancies. prior studies on patients with multiple sclerosis (ms) showed that 100% are ebv-seropositive and that their blood contains higher antibody titers than those of controls to both transformation and lytic cycle antigens. we performed three different assays for antibodies in csf to three major ebv antigens from patients with ms and controls. among 93 patients with ms, 79 (85%) had csf that r ... | 1992 | 1381067 |
| different localization of epstein-barr virus genome in two subclones of the burkitt lymphoma cell line namalwa. | the epstein-barr virus genome contained in the burkitt lymphoma line namalwa was previously localized to the short arm of chromosome 1. analysis of a different subline of the same namalwa line by means of southern analysis carried out on genomic dna, as well as in situ hybridization, showed a localization on the x chromosome. | 1992 | 1382560 |
| detection of, and anti-collagen antibody produced by, cd5-positive b cells in inflamed gingival tissues. | this study was performed to investigate the frequency and distribution of cd5-positive (cd5+) b cells in inflamed gingival tissues using flow cytometric and immunohistochemical analyses. the ability of cd5+ b cells to produce anti-type i collagen antibody was also examined. cd5+ b cells expressed "low" fluorescence intensity in the peripheral blood of both healthy subjects and patients with adult periodontitis. however, in inflamed gingival tissues the intensity of this surface marker was high. ... | 1992 | 1383487 |
| igm kappa/lambda ebv human b cell clone: an early step of differentiation of fetal b cells or a distinct b lineage? | in agreement with the clonal theory, one b lymphocyte synthesizes one antibody due to allelic and isotypic exclusion. we analyzed an ebv b-cell clone, e29.1, derived from an 11 week-old embryo, and secreting both igm kappa and igm lambda. structural analysis of produced igm, indicated that lambda-containing pentamers could be considered hybrid molecules, expressing both the kappa and lambda. chains, with a kappa/lambda ratio between 5 and 10. it was also found that 60% of the lambda chains were ... | 1992 | 1383695 |
| epstein-barr virus transcription factors. | | 1992 | 1327084 |
| mapping of an epitope defined by a human hybridoma antibody (trd3): a new hla-b supertype associated with a subset of hla-bw6. | the new human-human hybridoma trd3 secretes a cytotoxic igm mab, which reacted with 28 of a panel of 56 hla-typed lymphoblastoid cells. all 28 trd3+ cells expressed the hla-b supertype bw6, whereas 10 bw6+ cells were not recognized by the mab. none of the 17 bw4 homozygous cells were positive with trd3. thus, trd3 divided the bw6+ hla-b specificities of the cell lines into two subgroups, namely, bw6+trd3+ and bw6+trd3-, and therefore defines a new hla-b supertype. trd3 reacted strongly with some ... | 1992 | 1385373 |
| peptide-induced nonresponsiveness of hla-dp restricted human t cells reactive with dermatophagoides spp. (house dust mite). | the activation of cd4+ t lymphocytes, which play a central role in allergic inflammation, depends on the recognition of allergen-derived peptides in association with major histocompatibility complex class ii gene products. in this report we demonstrate, at a clonal level, that a component of the t-cell repertoire reactive with dermatophagoides spp. (house dust mite) in atopic individuals, is restricted by hla-dp class ii molecules. this supports the recent results emerging from genetic epidemiol ... | 1992 | 1385507 |
| human helper t cell lines established by coculture of normal human cord leukocytes with an htlv-ii-infected rabbit leukocyte cell line (ra-iia). | three helper t cell lines, designated cr-iia (cr-iia-1, cr-iia-2, and cr-iia-3), were established by coculturing normal human cord leukocytes with a lethally irradiated htlv-ii (human t-lymphotropic virus type ii)-infected rabbit leukocyte cell line (ra-iia). cr-iia had a normal human karyotype and expressed the surface markers cd3(+), cd4(+), cd8(-), cd19(-), cd25(+) and hla-dr(+), confirming their helper t cell nature. cr-iia cells were all free of epstein-barr virus nuclear antigen and were i ... | 1992 | 1386172 |
| quantitative oropharyngeal epstein-barr virus shedding in renal and cardiac transplant recipients: relationship to immunosuppressive therapy, serologic responses, and the risk of posttransplant lymphoproliferative disorder. | epstein-barr virus (ebv) infection plays a major role in the pathogenesis of posttransplant lymphoproliferative disorder (ptld). quantitative oropharyngeal ebv shedding measured by a dna-dna dot blot assay and the genotype of isolates determined by a polymerase chain reaction assay were studied in 23 renal and 23 cardiac transplant recipients followed over the first posttransplant year. five patients developed ptld and two additional ptld renal transplant recipients were studied from the time of ... | 1992 | 1328412 |
| viruses other than hiv and non-hodgkin's lymphoma. | there are several viral infections which are known to cause lymphoma among animals; all establish latency in lymphoid cells. the human t-lymphotropic virus type i is a human virus which causes lymphomas among a subset of carriers. however, this virus is very restricted in its distribution and as such, is unlikely to play a role in the increase of non-hodgkin's lymphoma (nhl). a highly prevalent infection, the epstein-barr virus (ebv) is known to play a role in the etiology of nhl among persons w ... | 1992 | 1394158 |
| epstein-barr virus (ebv) and human hematopoietic cell lines: a review. | two facts need to be pointed out to help explain why the history of epstein-barr virus (ebv) research has been inseparable from that of the studies with human hematopoietic cell lines of neoplastic and non-neoplastic origin. one is that burkitt lymphoma (bl) cell lines, ebv-positive or-negative, can be established in culture quite easily. thus, the bl cell lines which epstein established were indeed some of the first hematopoietic as well as virus-carrying cell lines of human neoplastic origin. ... | 1992 | 1329932 |
| chronic epstein-barr virus infection. | | 1992 | 1330851 |
| epstein-barr virus latent membrane protein transactivates the human immunodeficiency virus type 1 long terminal repeat through induction of nf-kappa b activity. | the epstein-barr virus latent membrane protein (lmp) is an integral membrane protein that is expressed in cells latently infected with the virus. lmp is believed to play an important role in epstein-barr virus transformation and has been shown to induce expression of several cellular proteins. we performed a series of experiments that demonstrated that lmp is an efficient transactivator of expression from the human immunodeficiency virus type 1 long terminal repeat (hiv-1 ltr). mutation or delet ... | 1992 | 1404600 |
| constitutive production of interferon-alpha 2 by a human b-lymphoblastoid cell line. | human b-lymphoblastoid cell lines immortalized by epstein-barr virus constitutively produce low levels of acid-stable interferon (ifn) that differs in composition from ifn produced by the same cell type after virus induction. we use three approaches to study the constitutive ifn produced by the lymphoblastoid cell line, lukii: (i) antiviral bioassays with a neutralizing monoclonal antibody; (ii) amino-terminal sequence analysis of the single protein obtained after purification of the ifn by immu ... | 1992 | 1431307 |
| quantitation of polymerase chain reaction products by hybridization-based assays with fluorescent, colorimetric, or chemiluminescent detection. | in this report two nonradioactive assays for quantitative analysis of polymerase chain reaction (pcr) products are presented. in the first assay, magnetic beads coated with streptavidin were used to capture biotinylated pcr fragments. after hybridization with a hapten-labeled probe, these beads were analyzed either by flow cytometry (method a) or by immunoenzymatic reactions (method b). using a dilution series of purified pcr products, we consistently found a lower detection limit of 1.5 fmol fo ... | 1992 | 1332534 |
| in situ hybridisation of ebv dna-dna hybrids using wet heat in polypropylene containers. | to explore procedures designed to optimise dna-dna in situ hybridisation, using cells infected with epstein-barr virus (ebv) and tissues and subfragments of the ebv dna as probes. | 1992 | 1336018 |
| human immunodeficiency virus as a risk factor in miscellaneous cancers. | the association of malignancies, such as non-hodgkin's lymphoma and kaposi's sarcoma, with human immunodeficiency virus infection has been recognized since the beginning of the epidemic. however, an increasing number of tumors not diagnostic of acquired immunodeficiency syndrome has been described in this setting. taking into consideration that survival of patients with human immunodeficiency virus infection is increasing because of improvement of supportive care and better control of human immu ... | 1992 | 1457506 |
| hairy leukoplakia with involvement of the buccal mucosa. | oral hairy leukoplakia occurs mainly on the tongue of human immunodeficiency virus (hiv)-infected persons. an hiv-infected patient with hairy leukoplakia involving the tongue and buccal mucosa was studied by light and electron microscopic methods, in situ hybridization, and polymerase chain reaction. our findings indicate that hairy leukoplakia may involve the buccal mucosa and should be considered in the differential diagnosis of white oral lesions in hiv-positive patients. epstein-barr virus p ... | 1992 | 1469145 |
| [chronic fatigue syndrome and virus infection: human herpesvirus 6 (hhv-6) infection]. | chronic fatigue syndrome (cfs) is newly-recognized disease characterized by chronic and debilitating fatigue. it has been suggested that viral infection may be involved in this syndrome from the results of clinical examination, including increased activity of 2',5'-synthetase in leukocytes of patients. the following viruses have been reported as etiologic agents of this disease. first, many studies have found elevated levels of igg to viral capsid antigen and early antigens to epstein-barr virus ... | 1992 | 1337558 |
| [human hybridoma neutralizing antibodies to the herpes simplex virus]. | human hybridoma cells producing monoclonal antibodies to human herpes simplex virus were generated. the antibody produced by the hybrid cells interacted with herpes virus types i and ii in enzyme immunoassay and complement fixation test, had neutralizing activity both in vivo and in vitro. these human monoclonal antibodies may be used for immunotherapy of herpes virus infection. | 1992 | 1337804 |
| density-dependent responsiveness to autocrine growth factors of epstein-barr virus transformed human b lymphocytes. | analysis of the growth requirements of epstein-barr virus (ebv)-transformed b lymphocytes shows that interleukin 1 and thioredoxin, a disulfide reducing enzyme, are able to induce a marked increase in dna synthesis in the early phases of in vitro culture. by contrast, interleukin 6 induces a steady increase in dna synthesis comparable to that observed with crude conditioned supernatant. furthermore, ebv-transformed b cells exhibit a density-dependent responsiveness to autocrine growth factors, t ... | 1992 | 1501588 |
| mutagenesis by apurinic sites in normal and ataxia telangiectasia human lymphoblastoid cells. | we used a shuttle vector based on the epstein-barr virus origin of plasmid replication (orip) to determine the types of mutations induced by depurination in human cells. plasmid dna was incubated at ph 2 at 40 degrees c for various times to induce up to 20 apurinic (ap) sites per 9.7-kb plasmid and electroporated into lymphoblastoid cells derived from either a normal individual or an ataxia telangiectasia patient. after replication of the vector in the human cells, plasmid dna was isolated and a ... | 1992 | 1503643 |
| cd43 is expressed normally on wiskott-aldrich-derived lymphocytes. | the wiskott-aldrich syndrome (was) is an x-linked disease characterized by eczema, thrombocytopenia, and profound immunodeficiency in affected males. while the etiology of the syndrome is currently unknown, abnormalities of cd43 have been described as a biochemical marker of the disease. several investigators have demonstrated alterations in the expression of the cd43 surface antigen on was hematopoietic cells, noting either absence, decreased levels or changes in the characteristic molecular we ... | 1992 | 1338889 |
| syncytial giant-cell hepatitis--a specific disease entity? | syncytial giant-cell hepatitis was recently reported to be related to a paramyxovirus and carried a poor prognosis. twelve patients with syncytial giant-cell hepatitis seen in an 8 1/2-year period in our institute were reviewed. seven patients had an identifiable aetiological cause: two had autoimmune chronic active hepatitis, one had primary sclerosing cholangitis and autoimmune chronic active hepatitis, two presented with prolonged jaundice after acute hepatitis a and b, one had chronic epstei ... | 1992 | 1506641 |
| epstein-barr viral latency and cell immortalization as targets for antisense oligomers. | the approach of using an antisense oligonucleotide to oppose the synthesis of a single selected viral gene product needed to maintain the ebv episome in non-virus-producing cells appears to be promising not only for possible cure of latent viral infection, but also for reversal of ebv-driven cell proliferation. it is also possible that targeting multiple latent viral genes might mount a synergistic effect that would prove to be more efficient at curing latent infection. however, further work is ... | 1992 | 1340114 |
| unusual oral presentation of non-hodgkin's lymphoma in association with hiv infection. | in 4.4% of human immunodeficiency virus-associated non-hodgkin's lymphoma the presenting lesion is seen in the mouth. often the lesion may clinically resemble a less sinister process, and a definitive diagnosis of lymphoma may be delayed. we describe three unusual cases of non-hodgkin's lymphoma, appearing intraorally in association with other oral lesions, in hiv-positive homosexual men. the three patients reported here were all diagnosed as having diffuse, large-cell malignant non-hodgkin's ly ... | 1992 | 1518649 |
| effects of cyclosporine on human b-cell lymphoma development in vivo. | cyclosporine (csa) is a potent immunosuppressive agent primarily affecting t-lymphocyte function. patients receive csa following organ transplantation to prevent rejection. these patients are at high risk for developing epstein-barr virus (ebv)-induced lymphoproliferative disease (lpd) or b-cell lymphoma (bcl). severe combined immunodeficient (scid) mice reconstituted with human peripheral blood leukocytes (pbl) develop fatal b-cell lymphomas of human origin following latent or active infection ... | 1992 | 1341239 |
| generation of human monoclonal antibodies that confer protection against pertussis toxin. | a panel of human monoclonal antibodies reactive with pertussis toxin has been generated by means of epstein-barr virus infection. one of these, the 3f11 monoclonal antibody, showed the ability to neutralize in vitro and in vivo the toxic effects of the toxin. western blot (immunoblot) analysis located the 3f11 epitope on the s3 subunit. | 1992 | 1541543 |
| malignant lymphoma associated with human aids and with siv-induced immunodeficiency in macaques. | malignant lymphomas associated with human (hiv) and simian (siv) immunodeficiency virus infections are reviewed and compared. recent observation of a high frequency of lymphomas in a series of cynomolgus macaques, highly immunodeficient after infection with sivsm(smm3) are described. in addition to the increased frequency in human and monkey aids, siv and hiv lymphomas share several important features. clinically and by histology they present as aggressive high-grade malignant tumors with a pred ... | 1992 | 1571194 |
| [hemolytic anemia due to cold-reacting antibodies: association with human immunodeficiency virus infection and non-hodgkin's lymphoma]. | hemolytic disease by cold-reacting antibodies is a rare form of hemolytic anemia. in most cases a subjacent cause is found. this disease is generally related with reactive lymphoproliferative processes (infections by the epstein-barr virus and cytomegalovirus) or uncontrolled lymphoproliferative disorders (lymphomas, waldenström disease, chronic lymphatic leukemia). the presence of high titers of cryoagglutinins have recently been described in patients infected by the human immunodeficiency viru ... | 1992 | 1583950 |
| biological markers associated with prolonged survival in african children maternally infected by the human immunodeficiency virus type 1. | sixteen children over the age of 5 years (group 1) have been identified out of 537 children infected by human immunodeficiency virus and born to hiv-infected mothers, in kigali, rwanda. they were followed up for 2 years and compared with 16 younger aids patients (group 2) and with 16 age- and gender-matched hiv-1 seronegative children (group 3). fourteen group 1 subjects had anti-hiv-1 igm which persisted during the entire study period, in 11 cases directed to hiv-1 envelope proteins. in vitro, ... | 1992 | 1599753 |
| establishment and characterization of macaca fascicularis lymphoblastoid cell lines. | a panel of cynomolgus macaque lymphoblastoid cell lines (lcl) was established by transforming peripheral blood mononuclear cells (pbmc) with herpesvirus papio (hvp), and selected lines were examined by flow cytometry. results indicate that hvp-transformed macaque lcl are phenotypically heterogeneous and resemble human epstein-barr virus (ebv)-transformed lcl in the abundant expression of major histocompatibility complex (mhc) class i and class ii molecules. at least some lines are of b cell orig ... | 1992 | 1602455 |
| excretion of the epstein-barr virus from the genital tract of men. | epstein-barr virus (ebv) dna was detected by polymerase chain reaction in urethral discharges from 10 (48%) of 21 men attending a clinic for sexually transmitted diseases. five of the 10 subjects were concurrently excreting virus from their oropharynx. these findings indicate that ebv is shed from more than one mucosal site in seropositive adult males. involvement of the genital tract raises the possibility of sexual transmission as well as ebv-induced genital tract pathology. | 1991 | 1645383 |
| an update of the classification and diagnostic criteria of oral lesions in hiv infection. eec-clearinghouse on oral problems related to hiv infection and who collaborating centre on oral manifestations of the human immunodeficiency virus. | | 1991 | 1645406 |
| inhibition of epstein-barr virus infection in vitro and in vivo by soluble cr2 (cd21) containing two short consensus repeats. | the extracellular domain of cr2, the epstein-barr virus (ebv)/c3d receptor of b lymphocytes, contains 15 or 16 tandemly arranged short consensus repeat elements (scr). recombinant cr2 proteins containing scr 1 and 2 fused to staphylococcus aureus protein a (pa-cr2) and to murine complement factor h scr 20 (cr2fh) were expressed in escherichia coli and in insect cells, respectively. these recombinant cr2 molecules retained functional activity as indicated by their ability to bind to c3dg in an en ... | 1991 | 1645784 |
| epstein-barr virus and carcinomas. expression of the viral genome in an undifferentiated gastric carcinoma. | the epstein-barr virus (ebv) is associated with undifferentiated nasopharyngeal carcinomas (npcs). recently, ebv dna has been demonstrated also in some cases of gastric carcinoma with similar morphology as undifferentiated npc. here we report on the expression of ebv genes in a gastric undifferentiated carcinoma of nasopharyngeal type (ucnt). the small ebv-encoded nuclear rnas, ebers, were expressed in all tumor cells. the bzlf1 transactivator protein, which disrupts viral latency, was detectabl ... | 1992 | 1342957 |
| characterization of the topography of epstein-barr virus infection in human immunodeficiency virus-associated lymphoid tissues. | a highly sensitive in situ hybridization methodology for epstein-barr virus (ebv) rna was used to determine the topography of ebv infection in 16 cases of human immunodeficiency virus-associated lymphoid tissues. four lymphomas, 11 persistent generalized lymphadenopathy (pgl) lymph nodes, and one lymphoepithelial cyst were studied. the pattern of ebv infection was diffuse in all lymphoma cases and predominantly interfollicular in pgl nodes. no discernable pattern of infection was present in the ... | 1992 | 1344820 |
| the epstein-barr virus encoded membrane protein (lmp) induces phenotypic changes in epithelial cells. | the epstein-barr virus (ebv)-encoded membrane protein, lmp, is expressed in a proportion of undifferentiated nasopharyngeal carcinomas (npc). previous studies have shown that the transfection of the gene encoding lmp into a human keratinocyte line, rhek-1, induces morphological alterations and a reduced expression of cytokeratins. we have analyzed immunophenotypic changes in the rhek-1 line following lmp-transfection and compared these changes with the phenotype of npc biopsies. we demonstrate a ... | 1992 | 1352076 |
| peritoneal sanctuary for human lymphopoiesis in scid mice injected with human peripheral blood lymphocytes from epstein-barr virus-negative donors. | the successful engraftment in scid mice of intraperitoneally (i.p.) injected human lymphocytes (hu-pbl-scid) and the failure of intravenously injected peripheral blood lymphocytes (pbl) directed the present study to investigate the early events of donor cell proliferation in the peritoneal cavity. we found focal lymphocyte engraftment together with histio-monocytic interleukin (il)-6+ cell phenotypes which must have been transferred with the human cell inoculum, which could explain certain immun ... | 1992 | 1359971 |
| surface and molecular expression of complement-receptor type 2 of neoplastic cd5+ b cells in chronic lymphocytic leukemia. | | 1992 | 1376070 |
| effect of transforming growth factor-beta 1 and -beta 2 on the proliferation of burkitt lymphoma and lymphoblastoid cell lines. | we tested the effect of transforming growth factor (tgf)-beta 1 and tgf-beta 2 on the proliferation of human b cell lines. the panel was selected to give information whether (1) their origin, (2) their phenotype, (3) their epstein-barr virus (ebv) carrier state, influence their responsiveness. the growth of lymphoblastoid cell lines (lcl) was not inhibited by tgf-beta 1. the ebv-carrying burkitt lymphoma (bl) lines, daudi, jijoye, rael but not raji were inhibited. three ebv-negative bl lines and ... | 1991 | 1646619 |
| insulin receptor gene expression is reduced in cells from a progeric patient. | we have studied a 15-year-old girl (p1) suffering from the hutchinson-gilford syndrome (progeria) associated with a severe insulin resistance. insulin binding activity to p1 erythrocytes was 85% reduced when compared to that measured in ten normal controls matched for sex and age. this finding was confirmed in epstein-barr virus (ebv)-transformed lymphoblasts and depends on a reduction in insulin receptor number. also the amount of total insulin receptors, [35s]methionine labeled and immunopreci ... | 1991 | 1646740 |
| presence of antibody to cyno-ebv in domestically bred cynomolgus monkeys (macaca fascicularis). | the presence of an antibody to the cyno-ebv was studied with cyno-ebv bearing cell line (ts-b-9) as antigen in 186 domestically bred cynomolgus monkeys (macaca fascicularis) ranging in age from birth to 18 yr as well as in 20 adult (older than 10 yr) cynomolgus monkeys of wild origin. the maternal antibody to cyno-ebv persisted for less than four mo after birth in infant monkeys. after one yr of age, all the domestically bred monkeys were consistently positive for cyno-ebv. in the monkeys of wil ... | 1991 | 1646886 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 29-1991. a 56-year-old woman with pneumoperitoneum three months after receiving a renal transplant. | | 1991 | 1646963 |
| an epstein-barr virus-producer line akata: establishment of the cell line and analysis of viral dna. | an epstein-barr virus (ebv)-producer line, designated akata, was established from a japanese patient with burkitt's lymphoma. the akata line possessed the burkitt's-type chromosome translocation, t(8q- ; 14q+), and was derived from the tumor cell. akata cells produced a large quantity of transforming virus upon treatment of cells with anti-immunoglobulin antibodies (takada, 1984). southern blot analysis of viral dna indicated that the akata ebv is nondefective and more representative of wild-typ ... | 1991 | 1647567 |
| suppressive t cell function of epstein-barr virus induced b cell activation in active behçet's disease. | b and t cell function were studied in 10 patients with active behçet's disease (bd) and in 10 normal subjects. peripheral b lymphocytes infected with epstein-barr virus (ebv) were cultured for 20 days in the presence or absence of autologous t cells. immunoglobulin m and g secretions into the supernatants were assessed with an enzyme-linked immunosorbent assay. the extent of suppression of ebv-induced b cell activation by autologous t cells was significantly decreased in active bd patients as co ... | 1991 | 1647911 |
| infectious mononucleosis in adolescents. | infectious mononucleosis is a clinical manifestation of primary ebv infection in adolescents, characterized by a triad of clinical, laboratory, and serologic features. the classic signs and symptoms are not seen in every patient; rather, the presentations tend to fit into one of three clinical forms (pharyngeal, glandular, or febrile). recognizing these syndromes provides a useful framework for anticipating the clinical course, complications, and differential diagnosis. nonclassic presentations ... | 1991 | 1648197 |
| spontaneous igm autoantibody production in vitro by b lymphocytes of normal human neonates. | human neonate b lymphocytes display unique phenotypic and functional characteristics: in addition to cd1c antigens, cd5+ and cd5- subsets both express activation markers such as cd23 and bac-1. they proliferate strongly in the presence of various lymphokines (ril-2, ril-4, low molecular weight bcgf), but differentiate poorly in the presence of the same lymphokines, pokeweed mitogen and epstein-barr virus. it has also been reported that human neonate b lymphocytes produce polyreactive autoantibod ... | 1992 | 1376488 |
| conserved patterns of somatic mutation and secondary vh gene rearrangement create aberrant ig-encoding genes in epstein-barr virus-transformed and normal human b lymphocytes. | expression of n-terminal-truncated ig heavy chains without normal light chain expression has been shown to occur in human b cell tumor lines, and to be due to diverse types of structural alteration within the expressed ig heavy and light chain genes. due to the tumor cell origin of these lines, generation of aberrant ig-encoding genes may only occur after malignant transformation, reflecting the release of the tumor b cell from the need to express functional ig for continued clonal proliferation ... | 1992 | 1377945 |
| chronic lymphocytic leukemia/small lymphocytic lymphoma with reed-sternberg-like cells and possible transformation to hodgkin's disease. mediation by epstein-barr virus. | the pathogenesis of reed-sternberg cells and variants (rs-h cells) found in rare cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (cll/sll) is unknown. we studied 13 such cases by immunohistochemistry and in situ hybridization for identification of epstein-barr virus (ebv) rna. the rs-h cells in five cases expressed the b-lineage marker cd20 and were negative for cd15. in two cases, the rs-h cells showed expression of both cd20 and cd15, whereas in another six cases, the cells we ... | 1992 | 1384376 |
| restricted epstein-barr virus protein expression in burkitt lymphoma is due to a different epstein-barr nuclear antigen 1 transcriptional initiation site. | epstein-barr virus (ebv) expresses six nuclear antigens (ebnas) and three integral latent membrane proteins (lmps) in latently infected growth-transformed b lymphoblastoid cell lines (lcls). in contrast, ebv protein expression in burkitt lymphoma tissue or in newly established burkitt lymphoma cell lines is frequently restricted to the ebv genome maintenance protein, ebna-1. ebna-1 expression in the absence of other ebnas and lmp-1 has been an enigma since, in lcls, all ebna mrnas are processed ... | 1991 | 1648738 |
| lymphomas following cardiac transplantation. case report and review of the literature. | the success of allogeneic organ transplantation is in great part due to pharmacologic advances in the area of immunosuppressive therapy. however, this achievement has been attained at the price of an unexpectedly high incidence of malignancies in this transplant population. lymphoid malignancies predominate in this and other immunodeficiency states. there is some controversy in the literature over the clonal or malignant nature of these proliferations. this paper presents a case of burkitt-like ... | 1991 | 1648879 |
| cloning and characterization of the human muscle phosphofructokinase gene. | a 35-kbp region of genomic dna encoding the human muscle phosphofructokinase (hpfk-m) gene including all of the coding exons (1-22) plus 2.2-kbp of 5'-flanking sequence has been cloned. the exon boundaries are the same as has been observed for the rabbit muscle phosphofructokinase (rpfk-m), the human liver phosphofructokinase (hpfk-l), and the mouse liver phosphofructokinase (mpfk-l) genes. characterization of the structure of the hpfk-m gene and its transcript in epstein-barr virus transformed ... | 1992 | 1388024 |
| polymerase chain reaction (pcr) for detection of ecori polymorphism at the thrb gene. | | 1991 | 1649460 |
| expression, structure, and function of the cd23 antigen. | | 1991 | 1649548 |
| the past is prologue: use of serum banks in cancer research. | in this paper, we emphasize the uses of serum banks in cancer research. these include not only case/control studies but also prospective seroepidemiological studies in which the development of a serological marker, such as a viral antibody or viral antigen, can be correlated with the subsequent development of cancer in either an active surveillance program or the use of cancer registries or hospital records. several different methods of application of the cohort technique are illustrated by stud ... | 1992 | 1394173 |
| extensive and selective mutation of a rearranged vh5 gene in human b cell chronic lymphocytic leukemia. | b cell chronic lymphocytic leukemia (cll) is the malignant, monoclonal equivalent of a human cd5+ b cell. previous studies have shown that the vh and vl genes rearranged and/or expressed in cll have few and apparently random mutations. however, in this study, we have found that the rearranged vh251 gene, one of the three-membered vh5 family, has extensive and selective mutations in b-cll cells. somatic mutation at the nucleotide level is 6.03% in b-clls whereas the somatic mutation levels are mu ... | 1992 | 1402653 |
| host cell phenotype-dependent methylation patterns of epstein-barr virus dna. | we have shown previously that the ebna 1 and latent membrane protein encoding regions of the epstein-barr virus (ebv) genome are highly methylated at ccgg sequences in the burkitt's lymphoma (bl)-derived cell line rael, but are unmethylated in a lymphoblastoid cell line (lcl) harbouring the same virus. to examine whether this is a regular phenomenon, we compared the methylation patterns of selected regions (bamhi c, w, h, m, e, k and n fragments) of ebv dna in representative ebv-carrying cell ty ... | 1991 | 1649897 |
| recurrent upper respiratory infection, persistent paravertebral thoracolumbar muscle spasm, and fatigue associated with chronic active epstein-barr virus infection. | | 1991 | 1650418 |
| [function, molecular structure and gene expression regulation of interleukin-10 (il-10)]. | interleukin-10 has a variety of biological activities. murine interleukin-10 inhibits cytokine production by th2 cells in the presence of macrophages, enhances t cell proliferation, sustains the viability of b cells in vitro, induces class ii mhc antigen expression on b cells, enhances mast cell proliferation in the presence of il-3 and/or il-4, and inhibits cytokine production by macrophages. human interleukin-10 inhibits cytokine production by human t cells and reduces antigen-specific human t ... | 1992 | 1433975 |
| epstein-barr virus in oropharyngeal and nasopharyngeal secretions of patients with nasopharyngeal carcinoma and control subjects. | the frequency of oropharyngeal excreters of the epstein-barr virus among patients with nasopharyngeal carcinoma in hong kong was compared with those of healthy adults in hong kong and california. 6 (3%) of 177 patients, 11 (12%) of 92 hong kong residents, and 20 (15%) of 132 californians were excreters. the virus was detected in the nasopharyngeal secretion of only 1 of 67 patients and in 2 of 73 healthy adults. no convincing evidence for neutralizing antibody in the throat wash and nasopharynge ... | 1991 | 1650762 |
| [hairy leukoplakia: current concepts]. | current data about oral hairy leukoplakia are reported. clinical manifestations, histological and ultrastructural features and pathogenic mechanisms are firstly described. then diagnosis are exposed. finally, management is discussed. | 1991 | 1651048 |
| epstein-barr virus transformation of cryopreserved lymphocytes: prolonged experience with technique. | | 1991 | 1651051 |
| salivary gland dysfunction. | clinically significant declines of salivary output are not a function of aging. complaints of oral dryness (xerostomia) are common in the elderly patient, however. this complaint is most often a consequence of medications; tricyclic antidepressants, certain antihypertensives, and anticholinergics can cause marked decreases in salivary flow rates. other medications such as diuretics may cause xerostomia without decreasing salivary output. systemic diseases such as sjögren's syndrome can destroy s ... | 1992 | 1504941 |
| cooperation of ebv dna polymerase and ea-d(bmrf1) in vitro and colocalization in nuclei of infected cells. | expression of the epstein-barr virus (ebv) dna polymerase (ebvpol) open reading frame (balf5) by in vitro transcription-translation yielded a 116-kda primary translation product. enzymatic dna polymerase activity of the in vitro translated polypeptide required the presence of the 47-kda bmrf1 (ea-d) gene product. antiserum raised to the balf5 gene product expressed in escherichia coli specifically precipitated a 116-kda polypeptide in extracts of latently infected lymphoblastoid cells induced fo ... | 1991 | 1651595 |
| expression of recombinant calf prochymosin in mammalian cell culture. | the calf preprochymosin cdna was cloned into an extrachromosomal mammalian cell expression vector containing epstein-barr virus sequences using polymerase chain reaction. transfection of hela cells yielded hygromycin b resistant cell clones, expressing immunoreactive prochymosin, which was quantitatively secreted into the culture medium. based on western blotting we estimated that selected cell clones produced about 10-20 mg prochymosin per liter in 20 h. the biological activity of the secreted ... | 1991 | 1369334 |
| expression of cd45 isoforms by epstein-barr virus-transformed human b lymphocytes. | cd45 is the most common protein tyrosine phosphatase (ptpase) in the membrane of white blood cells, serving as a potent regulator of lymphocyte activation and signal transduction. while the amino acid sequence of the intracellular domain of the molecule is conserved, that of the extracellular domain occurs in multiple isoforms, each of the result of alternative mrna splicing. in t lymphocytes, the lowest relative molecular mass (mr) form, cd45ro, is associated with acquisition of memory function ... | 1992 | 1370260 |
| evidence for molecular subtypes of hiv-associated lymphoma: division into peripheral monoclonal, polyclonal and central nervous system lymphoma. | the pathogenesis of the hiv-associated lymphomas is not well understood. in order to begin characterizing this class of lymphoma, we initiated a molecular genetic study of dna extracted from 31 diagnostic biopsy specimens from patients diagnosed with aids-associated non-hodgkin's lymphoma. analysis of 25 peripheral lymphomas showed that 14 were monoclonal b-cell processes, while 11 appeared to be of polyclonal origin. five of the 14 monoclonal lymphomas were found to have rearrangements of the c ... | 1991 | 1652978 |
| the latent membrane protein-1 in epstein-barr virus-transformed lymphoblastoid cells is found with ubiquitin-protein conjugates and heat-shock protein 70 in lysosomes oriented around the microtubule organizing centre. | immunofluorescence studies on epstein-barr virus (ebv)-transformed lymphoblastoid cells have previously shown that the latent membrane transforming protein (lmp-1) is found in patch-like inclusions which also immunostain for vimentin. we now show that ebv transformation causes a major reorganization of intermediate filaments, microtubules, mitochondria, and lysosomal elements, which generally become oriented around the microtubule organizing centre. immunogold electron microscopy shows that lmp- ... | 1991 | 1653831 |
| [epstein-barr virus (ebv) in lymphoproliferative diseases]. | the results of dna or rna study in ebv-associated lymphoproliferative diseases were shown. for detecting ebv dna, southern blot analysis with bam hiw probe (internal repeat) and non-repeat (least often deleted) probe are used. probes close to (ex. lmp) or within terminal repeat can indicate clonality in terms of junctional structure. several such examples were shown, in which benign polyclonal ebv (+) cd3 + 8+ lymphocytes, ebv (+) cd3 + 4 - 8- granulay lymphocytosis, ebv (+) t(14, 22) b-cell lym ... | 1991 | 1653862 |
| human herpesvirus-6 infection may predispose cells to superinfection by other viruses. | double infection of cells by hhv-6, epstein-barr virus (ebv) and by human immunodeficiency virus (hiv-1) can enhance viral effects though genetic transactivation. it remained to be clarified, however, by which mechanism different viruses may enter the same cell. we have shown that hhv-6 infection of immature lymphoid cells rigidifies the cytoplasmic membrane and causes receptor proteins for viruses such as cd4 for hiv-1 and cr2 for ebv to be expressed. in our experiments, hhv-6 infected cells we ... | 1991 | 1654148 |
| identification of the human herpesvirus 6 glycoprotein h and putative large tegument protein genes. | determination of the nucleotide sequences of two molecular clones of human herpesvirus 6 (hhv-6) (strain gs) and comparison with those of human cytomegalovirus (hcmv) has allowed the identification of the genes for the glycoprotein h (gh) and the putative large tegument protein of hhv-6. two molecular clones of fragments of hhv-6, the bamhi-g fragment (7,981 bp) of the clone termed pzvb43 and a hindiii fragment (8,717 bp) of the clone termed pzvh14, represent approximately 10% of the hhv-6 genom ... | 1991 | 1654455 |
| a pathologic study of childhood lymphoma in taiwan with special reference to peripheral t-cell lymphoma and the association with epstein-barr viral infection. | the authors retrospectively reviewed the clinicopathologic and immunologic features of 65 consecutive cases of childhood lymphoma reported between 1980 and 1989. southern blot hybridization was also performed in 23 cases to study their association with epstein-barr virus (ebv) and human t-cell leukemia virus type 1 (htlv-1). the 65 cases included 56 non-hodgkin's lymphoma (nhl) (86%) and 9 hodgkin's disease (hd) (14%). the nhl could be classified into the following groups: group i, small nonclea ... | 1991 | 1655230 |
| production of islet cell antibodies from epstein-barr virus-transformed peripheral blood lymphocytes in type 1 (insulin-dependent) diabetic patients. | islet cell antibodies are usually detected in the sera of almost all type 1 (insulin-dependent) diabetic patients within several months after onset of the disease. the antibodies then disappeared quite early during the course of the disease. the present study was undertaken to detect islet cell antibody-producing clones in peripheral blood lymphocytes of type 1 diabetic patients whose islet cell antibodies could not be detected in sera. epstein-barr virus-transformed lymphocytes were employed to ... | 1991 | 1655542 |
| transcriptional activity across the epstein-barr virus genome in raji cells during latency and after induction of an abortive lytic cycle. | we have studied the relative rate of transcription across the epstein-barr virus genome in the burkitt's lymphoma cell line raji by nuclear run-on analysis during latency and after induction of an abortive lytic cycle with 12-0-tetradecanoylphorbol 13-acetate (tpa) and 5-iodo-2'-deoxyuridine (iudr). during latency the entire, or almost the entire, viral genome was found to be transcriptionally active to a low or intermediate extent, with some variation in activity along the genome. the fragment ... | 1991 | 1655954 |
| clinical and serological studies in a series of 45 patients with guillain-barré syndrome. | we retrospectively reviewed the clinical files of 45 guillain-barré syndrome (gbs) patients admitted to our department between 1979 and 1989. the age distribution was bimodal with a first peak in young adults (20-40 years), and a second one between 60 to 70 years. seasonal distribution showed a late fall and a hivernal predominance. three patients experienced a second attack of gbs 2-9 years after the first one. thirty-one (69%) presented antecedent events, most often a respiratory tract infecti ... | 1991 | 1655983 |
| the specificity of recognition of a cytotoxic t lymphocyte epitope. | an epstein-barr virus (ebv)-specific cd8+ cytotoxic t lymphocyte (ctl) clone (lc13) was shown to recognize the minimal peptide determinant flrgraygl from the ebna 3 antigen of the bl74 strain of ebv. the equivalent epitope from the b95-8 strain (flrgraygi) is not recognized when endogenously presented and the peptide is 15-fold less active than flrgraygl. a replacement set of peptides was synthesized in which each residue within flrgraygl was sequentially replaced with all other genetically code ... | 1992 | 1370414 |
| molecular analysis of the induction of immunoglobulin e synthesis in human b cells by interleukin 4 and engagement of cd40 antigen. | the molecular events leading to immunoglobulin e (ige) synthesis in human sige- b cells stimulated with interleukin 4 (il-4) and anti-cd40 monoclonal antibody (mab) 626.1 were analyzed. anti-cd40 mab increased the levels of il-4-induced germline c epsilon transcripts and induced the production of mature c epsilon mrna. these effects were dependent on the presence of il-4. nested primer pcr revealed deletional switch recombination occurring only in b cell stimulated with both il-4 and anti-cd40 m ... | 1992 | 1370532 |
| failure of epstein-barr virus-specific cytotoxic t lymphocytes to lyse b cells transformed with the b95-8 strain is mapped to an epitope that associates with the hla-b8 antigen. | there are two types, a and b, of epstein-barr virus (ebv) and b95-8 represents the common type a laboratory strain. herein, we show in a family study that paternal ebv-specific cytotoxic t lymphocytes (ctl) generated in short-term cultures following stimulation with the autologous b95-8-transformed lymphoblastoid cell line (lcl) or b cells freshly infected with the b95-8 isolate did not lyse haploidentical b95-8 lcl expressing the hla-a1, -b8, -dr3 paternal haplotype. in contrast, the haploident ... | 1992 | 1370774 |
| non-hodgkin's lymphomas in patients with human immunodeficiency virus infection. presence of epstein-barr virus by in situ hybridization, clinical presentation, and follow-up. | the authors studied all patients with serologic evidence of human immunodeficiency virus (hiv) infection and malignant non-hodgkin's lymphoma (nhl) that presented at a single hospital from 1982 to 1989. sixteen patients were identified, all white homosexual men with a mean age of 38.2 years. lymphoma was the initial presentation of hiv infection in 37.5%. sixty-two percent of the cases had a high-grade nhl, 31% had intermediate-grade, and 6% (one patient) had a low-grade lymphoplasmacytoid lymph ... | 1991 | 1657357 |