| [occurrences of human papilloma virus dna in cervical carcinomas]. | out of 16 cases involving a cervical carcinoma that were investigated by southern blot hybridization, found were human papilloma virus (hpv) types 16 and 18 dna sequences in 8 (50%) and in one (6.3%), respectively. six out of the 8 hpv 16-positive specimens were from squamous cell carcinomas, one was from an adenocarcinoma, and the remaining specimen was from an argyrophil small cell carcinoma. in 7 out of 9 hpv-positive specimens, the viral sequences were integrated in the tumor cell genome, wh ... | 1989 | 2550685 |
| genital human papillomavirus infections. | genital human papillomavirus (hpv) infection is an increasingly common sexually transmitted disease that has been associated with carcinoma of the cervix. genital warts are easily diagnosed by clinical criteria; however, the more prevalent subclinical infections generally require laboratory diagnosis. satisfactory methods for culture of hpv do not exist, and cytologic and histopathologic methods are the most widely available diagnostic techniques. detection of hpv dna is a more sensitive method, ... | 1989 | 2551567 |
| cytopathology of sexually transmitted disease. | although other methods exist for routine identification of most sexually transmitted diseases, the pathologist can often recognize the etiologic agents or their cytologic effects on papanicolaou (pap) smear. useful clinical information requires both the ability to recognize these effects and knowledge of their limitations. pap smear changes characteristic of trichomonas vaginalis, chlamydia trachomatis, herpes simplex virus, gardnerella vaginalis, candida sp., and human papillomavirus infections ... | 1989 | 2551568 |
| two independently transforming functions of human papillomavirus 8. | | 1989 | 2551581 |
| immunological status to epstein-barr virus and cytomegalovirus in patients with genital condylomata. | serological patterns against epstein-barr virus (ebv) and cytomegalovirus (cmv) were determined in patients with genital condylomata (gc). the ig g antibody values to eb-induced virus capsidic antigens (vca), early antigens (ea) and ig m to vca were significantly higher in the study group than in the controls. moreover, the concomitant presence of ebv-ig g anti-vca greater than or equal to 1/320, ebv-ig g anti-ea greater than or equal to 1/20 and ebv-ig m anti vca greater than or equal to 1/20 w ... | 1989 | 2551722 |
| detection of human papilloma viruses in paraffin wax sections with biotinylated synthetic oligonucleotide probes and immunogold staining. | human papilloma virus was detected by in situ hybridisation in routinely processed paraffin wax sections using a synthetically produced oligonucleotide probe, end-labelled with biotin, and amplified with anti-biotin-immunogold silver staining (anti-biotin-igss). this system proved more sensitive than amplification with streptavidin-biotinylated alkaline phosphatase for detecting human papilloma virus type 16 in cervical tissues. the method was successfully combined with antigen staining for papi ... | 1989 | 2551931 |
| genital human papillomavirus infection in panama city prostitutes. | little is known of the natural history of genital human papillomavirus (hpv) infections in women from high-risk populations. samples were collected from 183 panama city prostitutes and assessed for hpv (filter in situ dna hybridization) and for sexually transmitted agents. the cohort was followed for 8 mo; 51% of subjects completed four monthly return visits and 16% were sampled eight times. the proportion of women found infected with hpv increased significantly with increasing numbers of consec ... | 1989 | 2551974 |
| localization of human papillomavirus type 16 dna using the polymerase chain reaction in the cervix uteri of women with cervical intraepithelial neoplasia. | the localization of human papillomavirus type 16 (hpv-16) dna throughout the cervix uteri of women with cervical intraepithelial neoplasia (cin) was studied by utilizing the polymerase chain reaction technique directly on histologically defined sections of paraffin-embedded cervical tissue obtained by conizations. hpv-16 dna was detected only in the sections that contained cin lesions and/or koilocytes. no hpv-16 dna was detected in sections that contained only normal epithelium. this is in acco ... | 1989 | 2552002 |
| heterogeneity of the human papillomavirus group. | | 1989 | 2552162 |
| human papillomavirus type 29 (hpv-29), an hpv type cross-hybridizing with hpv-2 and with hpv-3-related types. | the cloning and partial characterization of human papillomavirus (hpv) type 29 is presented. by hybridization analyses, this virus appears to be related to hpv types associated with common warts and hpv types associated with flat warts. | 1989 | 2552165 |
| human papillomavirus (hpv) type 50, a type associated with epidermodysplasia verruciformis (ev) and only weakly related to other ev-specific hpvs. | the cloning and partial characterization of the genome of human papillomavirus type 50 is presented. alignment of the genomic map with that of human papillomavirus type 5, with which it is only weakly related, was permitted by partial dna sequence analysis. | 1989 | 2552168 |
| a rapid dna probe test for detecting human papilloma virus types 6/11 and 16 in biopsy specimens. | a simple, rapid and sensitive non-radioactive in situ hybridization assay for human papillomavirus (hpv) has been developed and used to detect hpv 6/11 and hpv16 dna in ano-genital biopsy specimens. a comparative study to determine the sensitivity and the specificity of this assay relative to southern blot hybridization assays was performed using one hundred biopsy specimens. the sensitivity of the in situ hybridization assay was 88% for hpv type 6/11 and 89% for hpv type 16. the specificity of ... | 1989 | 2552303 |
| characterization of cdnas of spliced hpv-11 e2 mrna and other hpv mrnas recovered via retrovirus-mediated gene transfer. | human papillomaviruses (hpvs) are associated with hyperproliferations of cutaneous or mucosal epithelium. these viruses cannot be propagated in any cell culture system. because cloning cdna copies of hpv mrnas recovered from human lesions has met with only very limited success, the characterization of hpv mrnas has been problematic. using the moloney murine leukemia virus vector system (c.l. cepko, b.e. roberts, and r.c. mulligan, 1984, cell 37, 1053-1062), we have recovered cdnas of spliced e2 ... | 1989 | 2552658 |
| chronic cavitary respiratory papillomatosis. | a 19-year-old white man with multiple recurrences of respiratory papillomatosis was admitted for recurrent left lower lobe pneumonia and lung abscesses. he was found to have a single large laryngeal papilloma, widespread bronchial papillomatosis, and large cavitary lesions of the left lower lobe. a lobectomy was performed. the smooth-walled, squamous-lined cavities contained large numbers of papillomas, which were strongly positive for human papillomavirus type 11 by in situ dna hybridization. f ... | 1989 | 2552955 |
| detection of human papillomavirus (hpv) dna in focal epithelial hyperplasia. | five focal epithelial hyperplasia (feh) specimens from four patients were examined by southern blot hybridization analysis to determine the specific human papillomavirus (hpv) types present. the histomorphologic features of these specimens were also evaluated and a broad variety of changes including koilocytes, mitosoid cells, ballooning cells and cells showing individual cell keratinization were noted. feh lesions from the three patients sharing a familial relationship demonstrated hpv dna sequ ... | 1989 | 2547947 |
| genital human papillomavirus infection. | | 1989 | 2544328 |
| histogenesis of cervical adenocarcinoma with reference to human papillomavirus-18 as a carcinogen. | human papillomavirus (hpv) dna is present in adenocarcinoma of the cervix as frequently as in squamous cell carcinoma of the cervix. recent molecular biologic studies strongly suggested that hpv acts at least as a cocarcinogen in the female genital organs. thus, a study of the presence of hpv dna in adenocarcinoma in situ, endocervical glandular dysplasia and microglandular endocervical hyperplasia, three lesions suggested as possible precursor lesions of adenocarcinoma of the cervix, would clar ... | 1989 | 2553963 |
| structural analysis of human papillomavirus type 6c isolates from condyloma acuminatum and juvenile-onset and adult-onset laryngeal papillomata. | the human papillomavirus type 6c (hpv-6c) genome was molecularly cloned from biopsy specimens of a juvenile-onset and an adult-onset respiratory-tract papillomata and a condyloma acuminatum of the cervix. to determine if the genital-tract isolate and respiratory-tract isolates contain divergent sequences that may account for a difference in tissue trophism or for a difference in the age of onset of the disease, fine-structure mapping, heteroduplex analysis by electron microscopy, and nucleotide ... | 1989 | 2554585 |
| episomal hpv 16 dna isolated from a cervical carcinoma presents a partial duplication of the early region. | an invasive cervical carcinoma was found to harbor an episomal variant of human papillomavirus (hpv) type 16 dna, with a size of about 10.1 kb. a genomic library of the tumor was constructed in bacteriophage lambda and a recombinant phage clone was isolated by screening with hpv 16 probe. analysis by restriction mapping and southern hybridization showed that the isolate contained a 2.2 kb duplication of the early region, which included part of e6, all e7 and part of e1 open reading frames. possi ... | 1989 | 2554613 |
| virology of human papillomavirus. | | 1989 | 2544331 |
| characterization of normal human exocervical epithelial cells immortalized in vitro by papillomavirus types 16 and 18 dna. | an in vitro system for studying the interaction between human papillomavirus (hpv) 16 and 18 recombinant dna and normal human exocervical epithelial cells is described. eight hpv-immortalized human exocervical epithelial cell lines were established; all the lines contained either integrated hpv16 or 18 sequences and expressed hpv mrnas. thus, integration and expression appear to be required for immortalization. immortalized cells (greater than 200 population doublings to date) divided rapidly (d ... | 1988 | 2456144 |
| [bladder carcinoma in situ which harbored human papilloma virus. a case report]. | last year, we reported that human papilloma virus type 16 genome (hpv 16 genome) was detected in a case (s.y.) of bladder carcinoma in situ (bladder cis) (cancer res., 1988). since then, a number of bladder tumors other than cis were searched for hpv genome. however, no hpv genome was detected in the bladder tumors. from the results, we consider that hpv may not have a relation with all types of bladder tumor but with only a part of it. in the current report, the case (s.y.) is presented more pr ... | 1989 | 2555617 |
| analysis of hpv-1 e4 gene expression using epitope-defined antibodies. | six monoclonal antibodies (mabs) have been raised against the e4 proteins of hpv-1. five of these were found to recognize denaturation-resistant epitopes as determined by western blotting--and their binding sites were identified by determining their reactivity against a panel of bacterial e4--beta-galactosidase fusion proteins which contained progressive deletions at the c-terminal end of the e4 region. the five mabs were found to bind to four distinct sites. by using these epitope-defined mabs, ... | 1988 | 2456213 |
| structure and expression of an integrated human papillomavirus type 16 genome amplified in a cervical carcinoma cell line. | a cellular sequence containing the integrated human papillomavirus type 16 genome in a cervical carcinoma cell line qg-u was cloned and analysed. the transcriptionally active viral genome disrupted at the e2 and l2 open reading frames was amplified with its flanking sequences. | 1989 | 2544671 |
| epidemiology of human papillomavirus (hpv) infections and their associations with genital squamous cell cancer. review article. | reliable assessment of the epidemiology of genital hpv infections is hamphered by a number of technical problems. because of the lack of tissue-culture systems, methods based on morphological approaches (colposcopy, cytology and histopathology) play a central role in hpv diagnosis. even dna-hybridization techniques and the recently introduced dna amplification with pcr are extremely difficult to standardize, and are thus subject to major interlaboratory variation. further confusion in the field ... | 1989 | 2556164 |
| detection of human papillomavirus dna in cervical cancer tissue by the polymerase chain reaction. | a method for detecting hpv dna in cervical cancer tissue was developed without using isotopes. the dna samples from the cancer tissues were first subjected to amplification by pcr, followed by polyacrylamide gel electrophoresis to identify the specific amplified fragment. the specificity and sensitivity of the pcr method are described. compared with the dot hybridization technique, it is shown that the method is able to detect hpv dna in cervical cancer tissues. | 1989 | 2556422 |
| loss of leukoregulin up-regulation of natural killer but not lymphokine-activated killer lymphocytotoxicity in human papillomavirus 16 dna-immortalized cervical epithelial cells. | the sensitivity of human cervical epithelial cells immortalized by transfection with human papillomavirus type 16 (hpv16) dna, to lysis by natural killer (nk) and lymphokine-activated killer (lak) lymphocytes was evaluated at progressive stages of transformation. both early- (10-20 wk) and late- (greater than 30 wk) passage hpv16-immortalized cells were resistant to nk lymphocyte cytotoxicity but sensitive to lak lymphocyte cytotoxicity at lymphocyte-to-cervical cell ratios ranging from 1:1 to 5 ... | 1989 | 2544741 |
| novel variants of human papillomavirus type 2 in warts from immunocompromised individuals. | twenty cutaneous warts from i8 renal transplant recipients (rtr) and a single wart sample from a patient suffering from hodgkin's disease were screened for the presence of human papillomavirus (hpv) sequences by southern blot analysis. thirteen samples were shown to contain hpv dna sequences, one was identified as hpv-3, two as hpv-4, six as hpv-2 and four were unidentifiable by restriction digest analysis. hpv-3 was identified in the wart sample from the patient suffering from hodgkin's disease ... | 1989 | 2557072 |
| [intralesional therapy using recombinant interferon alpha 2 b in lesions of the uterine cervix caused by human papilloma virus]. | a clinical study on 20 patients with uterine cervix human papilloma virus (hpv) lesions treated with intralesional recombinant alpha 2b interferon (3 millions i.u.) three times a week for three weeks where possible, is presented. after one year from treatment, lesions were cured or stabilized in 18 patients (90%); progression was observed in 2 patients (10%) for which reason traditional surgical therapy was performed. | 1989 | 2549455 |
| trans-regulation and differential cell specificity of human papillomavirus types 16, 18, and 11 cis-acting elements. | the noncoding region (ncr) of human papillomavirus (hpv) types 16, 18, and 11 contains promoter and/or enhancer function. we have localized the sequence containing the constitutive enhancers of hpv types 16, 18, and 11 to 315, 230, and 213 bp fragments, respectively, for comparative studies. the region of homology shared between the enhancers of the three viruses is limited to the sequence atttttggctt, which is also present in the ncr of hpv 6b and 33. we have also examined the enhancer activity ... | 1989 | 2557381 |
| [detection of papilloma virus in the cervix of the human uterus by in situ hybridization--comparative study of detection rate by southern blot method]. | cases found to be positive for human papilloma virus (hpv) infection in the cervix by southern blot method were evaluated with a newly developed kit for in situ hpv tissue hybridization. by the in situ hybridization method, hpv-dna was detected without damaging the tissue structure. it appeared as purple or black stains in the nucleus of the epithelial cells that were located at the level of one-third of the epithelial thickness from the surface. the cases that were negative with the southern bl ... | 1989 | 2557467 |
| [warts and epidermoid carcinoma after renal transplantation]. | kidney transplant recipients suffer in the long-term from several cutaneous disorders linked to the transplantation. we had the opportunity to observe several patients presenting with pre-epitheliomatous keratoses and cutaneous carcinomas associated with warts. we report herein on five cases that were subjected to a clinical, histological and virological study. material and methods. clinical and histological report. the patients were referred to use by the kidney transplantation department of th ... | 1989 | 2545155 |
| screening for cervical carcinoma. | at present, cytological examination of cervical smears is the method used to screen the female population for cervical abnormalities. this employs the papanicolaou staining technique and cell morphology to detect abnormal cells from the susceptible transformation zone of the cervix. however, with the recent discovery of a possible link between human papillomavirus (hpv) infection and cervical cancer, a test to determine the presence of hpv infection may be appropriate. tests are still at a devel ... | 1989 | 2558271 |
| [microbiologic findings including chlamydia serology in cervix dysplasia and human papillomavirus infection]. | | 1989 | 2559001 |
| [two cases of oral leukoplakia possibly associated with human papillomavirus]. | two cases of oral leukoplakia were studied immunohistologically and ultrastructurally. by means of avidin-biotin peroxidase complex technique using papillomavirus specific antibody, distinct nuclear staining of epithelial cells of middle and upper layers was observed in both cases. further evidence for the presence of human papillomavirus was obtained by the electron micrograph, which revealed virus-like particles (30-40 nm in diameter) within the nucleus. | 1989 | 2559216 |
| squamous carcinoma of the lung in a nonirradiated, nonsmoking patient with juvenile laryngotracheal papillomatosis. | juvenile laryngotracheal papillomatosis (jltp)--a disease characterized by the growth of multiple, recurrent, koilocytolic papillomas of the upper respiratory tree--occurs in 1,500 to 2,000 infants and children in the united states every year. in rare instances, the papillomas, which are thought to be caused by a human papillomavirus (hpv), may extend into the distal bronchi and lungs. they are often excised surgically or by laser resection, but may regress spontaneously. patients who receive ra ... | 1987 | 2441616 |
| detection by antibody probes of human papillomavirus type 6 e5 proteins in respiratory papillomata. | we have demonstrated the expression of proteins arising from the e5a and e5b open reading frames (orfs) of human papillomavirus type 6c (hpv-6c) in respiratory tract papillomata. recombinant plasmids were constructed to express the orfs in the bacterial vectors path and prit2t. fusion proteins were purified and injected into rabbits to produce polyclonal antibodies. characterized antibodies generated against these fusion proteins were used in immunoperoxidase assays to identify the presence and ... | 1989 | 2559954 |
| the significance of atypical cervical cytology in an older population. | to ascertain the significance of squamous atypia encountered during routine papanicolaou smear screening in an older population, we reviewed 115 consecutive patients over age 50 seen during a 3-year period. evaluation included repeating the smear and performing colposcopy in all patients. colposcopically directed biopsy and endocervical curettage were performed when appropriate. sixty-seven patients (58.3%) had atrophy, 43 (37.4%) were normal, two (1.7%) had cervical intraepithelial neoplasia gr ... | 1989 | 2535763 |
| types of human papillomavirus isolated from japanese patients with epidermodysplasia verruciformis. | virological studies were performed on 12 patients with epidermodysplasia verruciformis (ev). three types of lesions were observed: red plaques, pityriasis versicolor (pv)-like macules and plane warts. human papillomavirus (hpv) 14, 20 and 21 were isolated from the plaques, hpv 3, 14 and 38 from flat warts and hpv 5, 12, 17, 20 and 38 from pv-like lesions. no clear relationship could be established between the different lesions and the types of hpv. types 17 and 20 have been isolated most frequen ... | 1989 | 2560401 |
| [study on molecular hybridization with biotin-labelled hpv 16 dna probe in human cervical carcinoma]. | biotin-labelled human papillomavirus (hpv) 16 type dna probe was prepared by the techniques of molecular biology. and dot hybridization technique was used to detect the hpv 16 homologous sequences in the tissues dna of human cervical carcinoma. the results indicated that 16 cases out of 28 of the human cervical carcinoma tissues were positive. the positive rate was 57%. the other 4 cases of normal uterine cervix tissues were negative. only 1 in 4 chronic cervicitis tissues showed positive. the h ... | 1989 | 2560458 |
| human papillomavirus 16 dna in cervical cancers and in lymph nodes of cervical cancer patients: a diagnostic marker for early metastases? | human papillomavirus (hpv) 16 is most prevalent in cervical cancers and also persists in metastases. we examined hpv16-dna-positive primary cancers and several lymph nodes from each of 14 patients to evaluate the use of hpv16 dna as a diagnostic marker for the detection of early node involvement. the hpv16 dna was exclusively integrated in 39% of the primary cancers, predominantly episomal in 36%, and integrated and extrachromosomal to a similar extent in 25%. thirteen of 16 involved lymph nodes ... | 1989 | 2536006 |
| cryotherapy and co2-laser vaporization in the treatment of cervical and vaginal human papillomavirus (hpv) infections. | a series of 119 women with human papillomavirus (hpv) infections of the uterine cervix and/or vagina were included in the present study, where the efficacy of cryotherapy and co2-laser vaporization was assessed after a mean follow-up of 14 months (sd 6 months) after treatment, as related to the natural history of the disease. routine papanicolaou (pap) smears with hpv-induced changes were the basis for patient recruitment. patients with cervical hpv lesions (hpv-ncin, hpv-cin i or ii) were rando ... | 1989 | 2561042 |
| evidence against diathermy as a beneficial treatment for human papillomavirus infection of the cervix. | an observational study of the influence of diathermy on the rate of subsequent cytological evidence of cervical intraepithelial neoplasia and/or human papillomavirus infection among women with a histological diagnosis of cervical human papillomavirus infection is presented. after the histological diagnosis of human papillomavirus infection, 35% (23/65) of women who were not diathermied had persistence/recurrence of cytological abnormalities compared with 30% (60/203) of the women who were diathe ... | 1989 | 2561048 |
| the multicentric nature of disease related to human papillomavirus infection of the female lower genital tract. | two hundred fifty-one consecutive patients from our colposcopy clinic were evaluated to establish the extent to which human papillomavirus (hpv) infection is a disease of the entire female lower genital tract. colposcopic examinations and biopsies of the cervix and vulva were performed on all patients. two hundred two women had cervical disease, of whom 164 (81%) also had vulvar disease. the percentage was the same regardless of the severity of the cervical disease. one hundred ninety-four of th ... | 1989 | 2536908 |
| production of transgenic mice carrying the human hepatitis b virus or the human papillomavirus dna sequences in taiwan: analysis of physical structure and hereditary mode. | in order to produce suitable animal model systems for the analysis of possible synergistic interaction between hepatitis b virus (hbv) or the human papillomavirus (hpv) with other viral or chemical co-factors, we have produced transgenic mice carrying hbv, hpv16 or hpv18. transfer of 3343 micro-injected one-cell (c57/bl6 x cba) f1 embryos into pseudo-pregnant icr recipients produced 181 pups. seventeen of 151 of the pups analysed were found to be transgenics. southern blot and slot blot analysis ... | 1989 | 2561574 |
| fast and accurate test for human papilloma virus. | | 1989 | 2561883 |
| occurrence of human papillomavirus type 16 dna sequences and c-myc oncogene alterations in uterine-cervix carcinoma. | using genetic engineering and molecular biology techniques, we have examined sixteen human carcinomas in the uterine-cervix tumors (the most frequent tumor in méxico, representing 34% of malignant tumors in women), for the presence of human papillomavirus type 16 (hpv-16) dna sequences and possible alterations of the cellular myc (c-myc) proto-oncogene. in this study we have analyzed cervical carcinomas from patient with clinical stage ii. we detected in 31% of these samples, the presence of hpv ... | 1989 | 2562235 |
| high rate of human papillomavirus type 16 infection in cytologically normal cervices. | | 1989 | 2563804 |
| relation between infection with a subtype of hpv16 and cervical neoplasia. | a variant (hpv16b) of the previously described human papillomavirus (hpv)16 (now called hpv16a) has been identified by polymerase chain reaction assays. compared with hpv16a, hpv16b has a 21bp deletion and several point mutations within the upstream regulatory region. all cervical carcinoma samples studied contained only hpv16a. hpv16b was found only rarely in cervical neoplasia but was common in the normal population. | 1989 | 2566780 |
| human papillomavirus (hpv) type 6 and 16 dna sequences in bronchial squamous cell carcinomas demonstrated by in situ dna hybridization. | a series of 131 routinely processed, paraffin-embedded biopsy specimens derived from the same number of patients with a bronchial squamous cell carcinoma were analyzed using in situ dna-hybridization technique with a probe cocktail containing 35s-labeled human papillomavirus (hpv) dna of types 6, 11, 16, 18, and 30. the 12 carcinomas shown to contain hpv dna by the probe cocktail were subjected to in situ hybridization with the specific hpv dna probes applied separately under high stringency con ... | 1989 | 2537916 |
| retraction: human papillomavirus subtype 16b. | | 1989 | 2574815 |
| [risk factors for condylomata. a case control study]. | the present study involves 214 patients whose cervical smear showed signs of human papilloma virus infection (hpv), matched with 1042 controls. we demonstrate increasing odds ratio with the total number of sexual partners since first sexual intercourse (p less than 0.0001). moreover the cases have more often changed partners in the year preceding the diagnosis (odds ratio 2.6 p less than 0.0001). their male partners more often have occupations that expose them to "extra-conjugal adventures" (p = ... | 1989 | 2600372 |
| correlation of the histologic appearance of intraepithelial neoplasia of the cervix with human papillomavirus types. emphasis on low grade lesions including so-called flat condyloma. | cervical condylomas and intraepithelial neoplasia (cin) were correlated with human papillomavirus (hpv) types and analyzed for the presence of abnormal mitotic figures. colposcopically directed cervical biopsies were divided in half and processed for routine microscopy and southern blot hybridization. of 83 specimens from 71 patients, 70 (84%) contained hpv-dna sequences. the hpv distribution was as follows: hpv 16 in 6/25 flat condylomas (fc), 2/8 cin i, 8/18 cin ii, 12/14 cin iii; hpv 18 in 1/ ... | 1989 | 2540101 |
| [assessment of human papilloma virus colposcopic findings of the cervix (in the italian colposcopic classification]. | hpv-induced flat condylomatosis is not only much more frequently observed on the hexocervix than the florid type, but it also induces colposcopic changes in the epithelium without completely altering its morphology. hpv-induced changes have now been largely identified and are recognisable in colposcopy. they can therefore be classified in the various patterns that characterise colposcopically the transformation epithelium, with undeniable advantages not only from the point of view of their corre ... | 1989 | 2671807 |
| perspectives on research and diseases of the tropics: an asian view. | diarrhea, pneumonia, and malnutrition account for most of mortality and morbidity in children in developing countries. the expanded program of immunization (epi) is making progress with more than 50% of children under the age of 1 year receiving vaccination against the 6 epi-listed diseases. the eradication of poliomyelitis by 2000 is realistic, so that the world could be smallpox- and polio-free by the 21st century. in july-august 1988 a cholera epidemic erupted in delhi, india in which sever ... | 1989 | 2698093 |
| cervical intraepithelial neoplasia in lymphoma patients: a cytological and colposcopic study. | twenty-seven patients with hodgkin's (n = 19) and non-hodgkin's (n = 8) lymphomas underwent cytological and colposcopic screening of the uterine cervix. colposcopically directed cervical punch biopsies were taken from all patients in whom a colposcopic abnormality was detected. lymphoma patients were compared with 79 controls with normal cervical cytology and no known haematological abnormality. colposcopically directed punch biopsies were taken from the cervical transformation zone of all contr ... | 1989 | 2713244 |
| [infection by the human immunodeficiency virus (hiv) in french guyana. dermato-venereologic problems]. | dermato-venereal manifestations in hiv infection and its severe evolution stage, aids, is of particular importance in tropical zones: we may be suspicious of the viral infection and consequently to request serologic tests to confirm it. we get an explanation of the virus transmission during heterosexual relations by the frequent occurrence and importance of the genital manifestations, leading to consider aids as a true sexually transmitted disease. beside the classical opportunistic infections, ... | 1989 | 2725241 |
| cervical cytology in a community laboratory. | a retrospective analysis of the results of 27,801 cervical smears from one year in a community laboratory was undertaken. this represented 23,820 individual patients. abnormalities were seen in 9.3% of patients. three point seven percent of patients had benign abnormalities, 5.5% had cervical intraepithelial neoplasia and 0.1% had major abnormalities. the incidence of cervical epithelial abnormality was highest in the 15-34 age group but major abnormalities were seen most often in the 55+ age gr ... | 1989 | 2739985 |
| malignant transformation of anorectal giant condyloma acuminatum (buschke-loewenstein tumor). | giant condyloma acuminatum, originally described by buschke and loewenstein in 1925 as a lesion of the penis, is more rarely seen in the anorectum and is characterized by clinical malignancy in the face of histologic benignity; however, malignant transformation to frankly invasive squamous-cell carcinoma has been described. malignant transformation has been reported in 15 patients with "ordinary" condylomata acuminata as well. twenty giant condylomata acuminata have been previously reported, six ... | 1989 | 2791784 |
| detection of human papillomavirus type 11 dna in a conjunctival squamous cell papilloma by in situ hybridization with biotinylated probes. | a 44-year-old man presented with an exophytic papillomatous growth in the tarsal conjunctiva of his right eye. the tumour was excised and subjected to histological examination. on light microscopy, a squamous cell papilloma without signs of dysplasia was disclosed, because the entire tissue block was cut into sections, the performance of a conventional in situ hybridization for detection of human papillomavirus (hpv) dna was not possible. an alternative approach was used to demonstrate the prese ... | 1989 | 2801046 |
| sexually transmitted pathogens in pregnant women in a rural south african community. | one hundred and ninety three consecutive pregnant women attending peripheral antenatal clinics attached to ngwelezana hospital, empangeni, kwa-zulu, were examined for evidence of sexually transmitted pathogens. the following incidences were found: trichomonas vaginalis 49.2% (95), candida spp 38.3% (74), chlamydia trachomatis 11.4% (22), gardnerella vaginalis 6.2% (12), neisseria gonorrhoeae 5.7% (11), positive syphilis serology results 11.9% (23), hepatitis b surface antigen 4.1% (eight). no wo ... | 1989 | 2807289 |
| spatula/cytobrush vs. spatula/cotton swab detection of cervical condylomatous lesions. | human papillomavirus is widely implicated as a primary etiologic factor in cervical carcinoma. this study compared the sampling accuracy of the modified ayre spatula/zelsmyr cytobrush versus the modified ayre spatula/cotton swab in the detection of cervical lesions with koilocytotic features. the histologic categories consisted of koilocytotic lesions (flat condylomas) with minimal cellular atypia and cervical intraepithelial neoplasia (cin) i, ii and iii with surface koilocytes showing cellular ... | 1989 | 2810246 |
| [oral contraceptives and cancer]. | recently some studies have shown an association between the pill and increased risk of breast cancer and cancer of the cervix. several well- designed prospective studies indicated that there was an increased risk of breast cancer for women without children with menarche before 13 who took the pill for many years. a control case study of 407 breast cancer victims compared 424 controls found that the risk of women who had taken hormonal contraceptives was double, however, the duration of use, ag ... | 1989 | 2814116 |
| human papillomavirus-associated lesions of the penis: colposcopy, cytology, and histology. | the diagnosis and therapy of human papillomavirus infections in men is of potential benefit because it may decrease the reservoir of virus from which genital condylomata or squamous neoplasia may arise in women. to investigate the efficacy of diagnostic methods, we examined the 127 male sexual partners of 127 women with cervical intraepithelial neoplasia. eighty-three mostly asymptomatic men (65%) had from one to 50 (median three) small (median 3 mm) human papillomavirus-associated lesions consi ... | 1987 | 2819795 |
| human papillomavirus deoxyribonucleic acid in lesions of the female genital tract: evidence for type 6/11 in squamous carcinoma of the vulva. | tissue specimens from 51 patients with genital condyloma acuminata or invasive cervical or vulvar carcinomas were analyzed for the presence of human papillomavirus deoxyribonucleic acid (dna) using the dot blot technique. of ten condylomas, 80% contained dna related to human papillomavirus 6 or 11. sixty percent had evidence of dna related to human papillomavirus 16, and 30% contained dna related to human papillomavirus 18. of 24 squamous cervical carcinomas, 58% had human papillomavirus type 16 ... | 1987 | 2819798 |
| detection of human papillomavirus-6 in primary carcinoma of the urethra in men. | the authors report a case of primary urethral carcinoma which seems to be linked to human papillomavirus (hpv) infection. southern blot hybridization of phosphorus 32 (32p)-labeled dna extracted from the cancer tissue gave a positive reaction with hpv-6. specimens of the tumor material subjected to in situ hybridization with 3h-labeled hpv-6 dna showed numerous tumor cell clusters with clearly labeled nuclei. using immunoperoxidase staining the authors found papillomavirus structural antigen wit ... | 1987 | 2820563 |
| cytologic and histologic manifestations of human papillomavirus infection of the female genital tract and their clinical significance. | the historical developments in the recognition of the role of human papillomavirus (hpv) infection in cells and tissues of the female genital tract are briefly summarized. the identification of a specific marker cell, the koilocyte, has led to initial studies of frequency and biologic significance of neoplastic lesions of the uterine cervix associated with hpv. by molecular virology techniques, over 40 types of hpv have been identified and their tissue affinity determined. types 6, 11, 16, 18, a ... | 1987 | 2820565 |
| causes and management of cervical intraepithelial neoplasia. | the evidence for human papillomavirus (hpv) playing a role in the pathogenesis of lower genital neoplasia is reviewed. it is likely that this group of viruses is etiologically important. it is suggested that cervical lesions be diagnosed as flat condyloma if they contain hpv types six or 11 and as cervical intraepithelial neoplasia (cin) if they are confined to the epithelium and contain hpv types 16 or 18 or other types associated with neoplasia. patients with a papanicolaou (pap) smear or clin ... | 1987 | 2820566 |
| human papillomavirus (hpv) infection in seborrheic keratosis. | we studied 89 seborrheic keratoses. in four cases, light microscopy and electron microscopy demonstrated that seborrheic keratoses may be associated with human papillomavirus (hpv) infection. | 1989 | 2543229 |
| human papillomavirus standardization and dna cytophotometry in cervical intraepithelial neoplasia. | human papillomavirus (hpv) standardization and dna cytophotometry were carried out in 29 cases of cervical intraepithelial neoplasia (cin) grades i-iii. a prognostically unfavorable dna distribution pattern with an aneuploid stemline was found in 14 of the 16 dysplasias with hpv types 16 and 18, while in 11 of 13 dysplasias with hpv types 6 and 11 there was a favorable dna distribution with a euploid-polyploid stemline. among 178 colposcopically, cytologically and histologically confirmed cervic ... | 1987 | 2821252 |
| case-control study of human papillomaviruses and cervical cancer in latin america. | human papillomavirus (hpv) types 16 and 18 have been implicated as risk factors for cervical dysplasia and neoplasia. however, most studies have been observational, uncontrolled and conducted in populations at low risk for invasive cancer. we report a pilot case-control study of incident invasive cervical cancer in panama, costa rica and bogota, colombia. between july and september 1985 we enrolled 46 consecutive newly diagnosed invasive cervical cancer cases and 51 age-matched control women. su ... | 1987 | 2822583 |
| human papillomavirus types 6 and 16 in cooperation with ha-ras transform secondary rat embryo fibroblasts. | using a focus assay we have shown that the entire human papillomavirus type 16 (hpv-16) genome is capable of cooperating with an activated ras oncogene to transform secondary rat embryo fibroblast (ref) cells as indicated by focus formation of unselected cells. however, this assay failed to detect any similar activity with either the whole hpv-6 genome or with subgenomic fragments. in contrast, transformed colonies appeared when g418 (geneticin)-resistant colonies were selected after cotransfect ... | 1989 | 2543741 |
| colposcopic correlates of cervical papillomavirus infection. | we compared the colposcopic and histologic features of 39 papillomavirus-related cervical lesions with their associated human papillomavirus type. two thirds of the lesions contained human papillomavirus type 16-related deoxyribonucleic acid, and 69% of them were classified as cervical intraepithelial neoplasia i or ii, in contrast to 92% of lesions containing human papillomavirus 6/11 deoxyribonucleic acid. on histologic examination, low-grade cervical intraepithelial neoplastic lesions tended ... | 1987 | 2823611 |
| the multicentric nature of disease related to human papillomavirus infection of the female lower genital tract. | | 1989 | 2543936 |
| practical approach to cervical human papillomavirus-related intraepithelial lesions. | members of the editorial board who have been active in the study of female genital hpv-related lesions have responded to a series of questions related to the histopathologic diagnosis of such lesions in the uterine cervix, the performance of auxiliary studies, therapeutic choices, and the correlation of cytologic and tissue diagnoses. | 1989 | 2553631 |
| human papillomaviruses in oral epithelial lesions. comparative study between histopathology and immunohistochemistry in routine diagnosis. | forty five oral papillary lesions classified clinically and histologically as condylomatous papilloma, hyperkeratotic papilloma and oral wart were subjected to histopathological evaluation and tested with human papilloma virus (hpv) antiserum, in order to detect hpv group-specific antigens. the immunoperoxidase labelling technique was positive in 10% of 31 condylomatous papillomas, 22% of 9 hyperkeratotic papillomas, and 60% of 5 oral warts. no viral antigens could be detected in 4 oral fibromas ... | 1987 | 2824453 |
| identification of human papillomavirus type 16 e7 protein by monoclonal antibodies. | a number of human papillomavirus (hpv) type 16 proteins have recently been identified in human cervical carcinoma cell lines using polyclonal antisera against papillomavirus gene products expressed in escherichia coli. e7 protein has been found to be the most abundant papillomavirus protein in these cells. here we describe a panel of monoclonal antibodies recognizing a 15k mr non-glycosylated cytoplasmic hpv-16 e7 protein. one of the antibodies cross-reacted with hpv-18 e7 protein. | 1987 | 2824670 |
| human cytomegalovirus (hcmv) enhances bovine papilloma virus (bpv) transformation in vitro. | infection of nih 3t3 cells with a combination of hcmv and bpv resulted in more foci than infection with bpv alone. foci were microscopically apparent at 4 days in the mixed infection and did not appear until 2 days later in the cultures infected with bpv alone. the enhancement was abolished by heat inactivation of the hcmv and also when the hcmv was replaced by a "mock inoculum." southern blot analysis of cellular dna from transformed cells showed a similar amount of extrachromosomal bpv dna in ... | 1987 | 2824682 |
| squamous atypia on cytology. the influence of age. | the optimal management of squamous atypia on cytology has not been determined. colposcopy has been recommended because of the high false-negative rate of cytology. to evaluate colposcopy, 1,074 consecutive, nonpregnant women presenting with squamous atypia on cytology were examined prospectively with colposcopy and simultaneous repeat cytology. no specific treatment was given prior to evaluation. biopsy and endocervical curettage were performed when clinically indicated. two hundred two women (1 ... | 1989 | 2553961 |
| detection of dna of human papillomavirus types 6/11 and 16/18 in cell scrapings of the uterine cervix by filter in situ hybridisation. correlation with cytology, colposcopy and histology. | the application of filter in situ hybridisation (fish) to detect the presence of the dna of human papillomavirus genotypes 6/11 and/or 16/18 in cell scrapings of the uterine cervix of 248 women in western australia is described. the results obtained by fish are related to cervical dysplasia as assessed by cytology, colposcopy and histology. the detection of hpv infection was more sensitive and specific by fish than by either histological/cytological evidence of an hpv cytopathic effect (koilocyt ... | 1987 | 2826222 |
| detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction. | human papilloma virus (hpv) dna sequences have been detected in paraffin-embedded tissue using an enzymatic in vitro amplification technique known as the polymerase chain reaction. amplification of a hpv dna sequence before its detection with a cdna probe significantly increases the rapidity as well as the sensitivity of detection such that a single 5-10-micron thick paraffin-embedded tissue section can be analyzed within 24 h. the assay specifically detected hpv 16 or 18 without crossreactivity ... | 1988 | 2826637 |
| physical state and biological activity of human papillomavirus genomes in precancerous lesions of the female genital tract. | the dna of distinct human papillomaviruses (hpvs) is regularly detected in the majority of human cervical carcinomas. in contrast to benign hpv-induced genital lesions, where the viral genomes are exclusively present as episomes, in cervical carcinomas hpv type 16 (hpv16) dna was found to be integrated into the host dna. in order to determine the physical state and expression of hpv dna sequences at different stages of tumour development, we analysed a series of cervical lesions (mild, moderate ... | 1988 | 2826665 |
| colposcopy is superior to cytology for the detection of early genital human papillomavirus infection. | of 2232 women with no cytologic evidence of intraepithelial neoplasia, 250 (11.2%) were positive for human papillomavirus deoxyribonucleic acid (dna) by filter in situ hybridization. in 150 of those human papillomavirus-positive patients, an adequate colposcopic examination of the cervix was possible; human papillomavirus infection was diagnosed in 104 women (70%). cervical cytology showed evidence of human papillomavirus infection in only 23 patients (15%). the following colposcopic features we ... | 1988 | 2827083 |
| [controlled study of cases of uterine cervix cancer and infection with human papillomavirus in latin america]. | | 1987 | 2827244 |
| human papillomavirus dna in a recurrent squamous carcinoma of the eyelid. | the polymerase chain reaction (pcr) is a molecular technique that amplifies specific target dna sequences in vitro to facilitate identification of dna. we have applied the pcr to a recurrent infiltrating, well-differentiated squamous carcinoma from the right lower eyelid of a 37-year-old woman. with primers specific for human papillomavirus (hpv) type 16 dna, pcr yielded a single band of amplified dna product. the product was positive, with a radiolabeled hpv type 16 probe on dot blot analysis. ... | 1989 | 2554865 |
| detection of human papillomaviruses 16-18 in cervical cells by molecular hybridization: relationship with morphonuclear cell image analyses. | forty-one feulgen-stained cervical imprint smears were analyzed by means of the samba 200 cell image processor in order to quantitatively score human papillomavirus (hpv) 16-18-induced morphonuclear modifications as assessed by morphometric, densitometric, and textural parameters. molecular hybridization technology using 16 and 18 type specific genetic probes made it possible to divide our series into three groups: group 1, containing noninfected smears; group 2, containing "suspicious", i.e., b ... | 1989 | 2555818 |
| humoral and cellular immunity to papillomavirus in patients with cervical dysplasia. | the cell-mediated and humoral immune responses to human papillomavirus (hpv) were tested in groups of patients with various degrees of cervical intraepithelial neoplasia (cin) using a lymphocyte proliferation assay (lpa) as a measure of circulating sensitised t-cells and an enzyme-linked immunosorbent assay (elisa) for antibodies. twenty-three of 92 patients (25%) gave stimulation indices (s.i.) greater than two to at least one of the several antigen preparations tested in the lpa. of 282 patien ... | 1988 | 2828535 |
| [epidemiology of human papillomavirus genital infections in women]. | epidemiological variations condylomata plana show annual fluctuations and vast geographical differences. the risk is obviously related to the number of sexual partners and their profession. | 1989 | 2555892 |
| papillomavirus in the vapor of carbon dioxide laser-treated verrucae. | vapor produced by the carbon dioxide laser during the vaporization of papillomavirus-infected verrucae was analyzed for viral dna content. two models were used for evaluation: an in vitro cutaneous bovine fibropapilloma and an in vivo human verruca model. four bovine fibropapillomas were exposed to various laser parameters with power densities of 38,200 to 130 w/cm2 and energy fluences of 3820 to 130 j/cm2. the generated vapor was collected in a chamber in line with a vacuum system. hybridizatio ... | 1988 | 2828703 |
| human papillomavirus. | | 1987 | 2829073 |
| clinical manifestations and natural history of genital human papillomavirus infection. | over the past decade, the ever-increasing volume of evidence implicating hpv types in genital neoplasia has stimulated much research interest into all aspects of the biology of this interesting group of viruses. this has led to the identification of an ever-increasing heterogeneity of hpv types. it is not surprising, therefore, that the clinical profile of disease associated with genital hpv types is much broader than previously recognized. knowledge of this clinical spectrum is mandatory to th ... | 1987 | 2829075 |
| genital human papillomavirus infection. a growing concern. | hpv infections of the genital tract are one of the most common sexually transmitted viral infections in the united states. data from std clinics and private physicians' offices reveal that genital warts, one manifestation of genital hpv infection, have been diagnosed more frequently in recent years. using a variety of diagnostic techniques, asymptomatic hpv infection has been identified in men and women and is probably much more common than clinically apparent infection. | 1987 | 2829076 |
| integration and transcription of human papillomavirus type 6 recombinant dna in mouse cells. | human papillomavirus (hpv) dna is found in nature mostly in an episomal form. however, in permanent cell lines established from cervical carcinomas in which hpv sequences are present, they are usually integrated in the host genome. in vitro studies of hpv have been hampered because of the difficulty of stably maintaining hpv sequences in transfected cells. we have cloned the entire hpv6 genome into three vectors: pml2, a derivative of pbr322 lacking the "poison sequences"; p142-6, a plasmid cont ... | 1987 | 2829459 |
| [genital papillomavirus lesions. coexistence in sexual partners, role in cancer of the cervix and value of colposcopy]. | since the human papillomavirus is transmissible sexually, an ever increasing number of male patients is seen in dermatology departments. it therefore seems to be important to evaluate the frequency of hpv-related genital lesions in both males and females. several recent studies have made it clear that concomitant lesions are very often observed in sexual partners. we have tried here to summarize the different data reported in the literature concerning the frequency of hpv lesions and their proba ... | 1987 | 2829688 |
| role of herpes simplex virus type-2 and human papilloma virus in penile cancers in singapore. | to detect the presence of herpes simplex virus type 2 (hsv-2) and human papilloma virus (hpv), common structural antigens in penile cancers, 20 squamous cell carcinomas were studied by immunohistochemical methods. all specimens were from singaporeans ranging in age from 44 to 69 years. none of the specimens contained hsv-2 and hpv common structural antigens. the reasons for the absence of hpv common antigen were discussed. the significance of the association of hpv with penile cancers is uncerta ... | 1987 | 2829697 |
| sexually transmitted cancers and viruses. | both cervical cancer and its precancerous state cervical intraepithelial neoplasia (cin) have the characteristics of being sexually transmitted. formerly herpes simplex virus (hsv) but more recently human papillomavirus (hpv) which both infect the cervix have been implicated in causation. the role of these viruses as possible initiators of cancer or as potential cofactors of cocarcinogens is discussed at the molecular level within the context of the disease process. | 1989 | 2550096 |
| sexual transmission of human papillomaviruses in heterosexual and male homosexual couples, studied by dna hybridisation. | the prevalence of human papillomavirus (hpv) 6, 11, 16, and 18 in 36 heterosexual couples and seven male homosexual couples with genital warts was investigated for evidence of sexual transmission of genital hpv. the prevalence of virus type and number of copies of viral genome equivalents/cell in the lesions were assessed, and the factors influencing transmission analysed. our results show that hpv 6 and, to a lesser extent, hpv 11 were the types most readily transmitted, and that transmission a ... | 1988 | 2831137 |
| isolation of a novel human papillomavirus (type 51) from a cervical condyloma. | we cloned the dna from a novel human papillomavirus (hpv) present in a cervical condyloma. when dna from this isolate was hybridized at high stringency with hpv types 1 through 50 (hpv-1 through hpv-50), it showed weak homology with hpv-6 and -16 and stronger homology with hpv-26. a detailed restriction endonuclease map was prepared which showed marked differences from the maps for other hpvs that have been isolated from the female genital tract. reassociation kinetic analysis revealed that hpv- ... | 1988 | 2831406 |
| human papillomavirus: implications for clinical medicine. | | 1988 | 2831766 |
| human papillomavirus and epithelial ovarian neoplasia. | in contrast to the strong association between human papillomavirus (hpv) and squamous cell carcinoma of the lower female genital tract, no viral dna had been found in epithelial ovarian carcinoma. recently, however, r. h. kaufman, j. bornstein, a. n. gordon, e. adam, a. l. kaplan, and k. adler-storthy [gynecol. oncol. 27, 340-349 (1987)] reported the detection of hpv-6 dna by in situ hybridization in 10 of 12 patients with advanced epithelial ovarian carcinoma. to further investigate the possibl ... | 1989 | 2548934 |