| illness in long-term travelers visiting geosentinel clinics. | length of travel appears to be associated with health risks. geosentinel surveillance network data for 4,039 long-term travelers (trip duration >6 months) seen after travel during june 1, 1996, through december 31, 2008, were compared with data for 24,807 short-term travelers (trip duration <1 month). long-term travelers traveled more often than short-term travelers for volunteer activities (39.7% vs. 7.0%) and business (25.2% vs. 13.8%). more long-term travelers were men (57.2% vs. 50.1%) and e ... | 2009 | 19891865 |
| identification of an avirulent entamoeba histolytica strain with unique trna-linked short tandem repeat markers. | highly polymorphic, non-coding short tandem repeats (str) are scattered between the trna genes in entamoeba histolytica in a unique tandemly arrayed organization. str markers that correlate with the virulence of individual e. histolytica strains have recently been reported. here we evaluated the usefulness of trna-linked str loci as genetic markers in identifying virulent and avirulent strains of e. histolytica from 37 japanese e. histolytica samples (12 diarrheic/dysenteric, 20 amebic liver abs ... | 2010 | 19895903 |
| histomonas meleagridis possesses three alpha-actinins immunogenic to its hosts. | histomonas meleagridis is a protozoan parasite known to cause histomonosis (syn. typhlohepatitis) in poultry. due to the economic losses which the disease entails, there is an urgency to understanding its pathogenesis. in the present investigation, three partial cdna sequences encoding heterogenic alpha-actinins previously identified for h. meleagridis were completed and characterized. these three h. meleagridis alpha-actinin isoforms were named alpha-actinin1, alpha-actinin2, and alpha-actinin3 ... | 2010 | 19896981 |
| localization of phosphatidylinositol (3,4,5)-trisphosphate to phagosomes in entamoeba histolytica achieved using glutathione s-transferase- and green fluorescent protein-tagged lipid biosensors. | entamoeba histolytica is an intestinal protozoan parasite that causes amoebic dysentery and liver abscess. phagocytosis by the parasite is a critical virulence process, since it is a prerequisite for tissue invasion and establishment of chronic infection. while the roles of many of the proteins that regulate phagocytosis-related signaling events in e. histolytica have been characterized, the functions of lipids in this cellular process remain largely unknown in this parasite. in other systems, p ... | 2010 | 19901063 |
| association of malnutrition with amebiasis. | observation of a cohort of preschool children in dhaka, bangladesh, is beginning to reveal the contributions of environment, host, and parasite to amebiasis. reviewed here are the associations and interactions of malnutrition, iga and interferon-gamma, human leukocyte antigen alleles, and parasite genotypes to the outcome of infection. future efforts aimed at understanding the mechanisms of these effects are described. | 2009 | 19906225 |
| cytotoxic effect of amide derivatives of trifluoromethionine against the enteric protozoan parasite entamoeba histolytica. | amoebiasis, caused by infection with the enteric protist entamoeba histolytica, is one of the major parasitic diseases. although metronidazole and its derivatives are currently employed in therapy, the paucity of effective drugs and potential clinical resistance necessitate the development of a novel drug. trifluoromethionine (tfm) is a promising lead compound for antiamoebic drugs. to potentiate the antiamoebic effect of tfm, we synthesised various amide derivatives of tfm and evaluated their c ... | 2010 | 19910163 |
| identification and partial characterization of a dynamin-like protein, ehdlp1, from the protist parasite entamoeba histolytica. | the dynamin superfamily of proteins includes a large repertoire of evolutionarily conserved gtpases that interact with different subcellular organelle membranes in eukaryotes. dynamins are thought to participate in a number of cellular processes involving membrane remodeling and scission. dynamin-like proteins (dlps) form a subfamily of this vast class and play important roles in cellular processes, such as mitochondrial fission, cytokinesis, and endocytosis. in the present study, a gene encodin ... | 2010 | 19915078 |
| ultrastructural changes on entamoeba histolytica hm1-imss caused by the flavan-3-ol, (-)-epicatechin. | the flavan-3-ol, (-)-epicatechin has been previously identified as the most important antiamoebic compound among the extracts from two medicinal plants: rubus coriifolius and geranium mexicanum. here we report the effects of epicatechin on entamoeba histolytica morphology, analyzed by electronic microscopy. e. histolytica trophozoites were incubated for 48 h at 37 degrees c in the presence of 1.9 microg/ml epicatechin and processed for electronic microscopy analysis. epicatechin induced nuclear ... | 2010 | 19918717 |
| characterization of two isotypes of l-threonine dehydratase from entamoeba histolytica. | the genome sequence of the enteric protozoan parasite entamoeba histolytica suggests that amino acid catabolism plays an important role in energy metabolism. in the present study, we described kinetic and regulatory properties of catabolic l-threonine and l-serine dehydratase (td) from e. histolytica. td catalyses the pyridoxal phosphate-dependent dehydrative deamination of l-threonine and l-serine to ammonia and keto acids (2-oxobutyrate and pyruvate, respectively). e. histolytica possesses two ... | 2010 | 19931317 |
| an ex-vivo human intestinal model to study entamoeba histolytica pathogenesis. | amoebiasis (a human intestinal infection affecting 50 million people every year) is caused by the protozoan parasite entamoeba histolytica. to study the molecular mechanisms underlying human colon invasion by e. histolytica, we have set up an ex vivo human colon model to study the early steps in amoebiasis. using scanning electron microscopy and histological analyses, we have established that e. histolytica caused the removal of the protective mucus coat during the first two hours of incubation, ... | 2009 | 19936071 |
| microwave assisted synthesis, characterization and biological evaluation of palladium and platinum complexes with azomethines. | reactions of 3-acetyl-2,5-dimethylthiophene with thiosemicarbazide and semicarbazide hydrochloride resulted in the formation of new heterocyclic ketimines, 3-acetyl-2,5-dimethylthiophene thiosemicarbazone (c(9)h(13)n(3)os(2) or l(1)h) and 3-acetyl-2,5- dimethylthiophene semicarbazone (c(9)h(13)n(3)os or l(2)h), respectively. the pd(ii) and pt(ii) complexes have been synthesized by mixing metal salts in 1:2 molar ratios with these ligands by using microwave as well as conventional heating method ... | 2010 | 19962340 |
| mitosomes in entamoeba histolytica contain a sulfate activation pathway. | hydrogenosomes and mitosomes are mitochondrion-related organelles in anaerobic/microaerophilic eukaryotes with highly reduced and divergent functions. the full diversity of their content and function, however, has not been fully determined. to understand the central role of mitosomes in entamoeba histolytica, a parasitic protozoon that causes amoebic dysentery and liver abscesses, we examined the proteomic profile of purified mitosomes. using 2 discontinuous percoll gradient centrifugation and m ... | 2009 | 19995967 |
| fulminant colitis and toxic myocarditis: a unifying cause? | | 2009 | 20006131 |
| amebiasis. | amebiasis is an important cause of death from parasitic disease worldwide. the causative organism is entamoeba histolytica, which has an infective cyst stage and a pathogenic and motile trophozoite stage. the clinical presentation can vary from an asymptomatic carrier state to fulminant colitis and colonic perforation. the majority of patients can be managed medically. however, a small percentage of patients require urgent exploration and resection with an associated high mortality rate. early r ... | 2007 | 20011359 |
| use of thermal melt curves to assess the quality of enzyme preparations. | this study sought to determine whether the quality of enzyme preparations can be determined from their melting curves, which may easily be obtained using a fluorescent probe and a standard reverse transcription-polymerase chain reaction (rt-pcr) machine. thermal melt data on 31 recombinant enzymes from plasmodium parasites were acquired by incrementally heating them to 90 degrees c and measuring unfolding with a fluorescent dye. activity assays specific to each enzyme were also performed. four o ... | 2010 | 20018159 |
| integrating horizontal gene transfer and common descent to depict evolution and contrast it with "common design". | horizontal gene transfer (hgt) and common descent interact in space and time. because events of hgt co-occur with phylogenetic evolution, it is difficult to depict evolutionary patterns graphically. tree-like representations of life's diversification are useful, but they ignore the significance of hgt in evolutionary history, particularly of unicellular organisms, ancestors of multicellular life. here we integrate the reticulated-tree model, ring of life, symbiogenesis whole-organism model, and ... | 2010 | 20021546 |
| phospholipid-binding protein ehc2a mediates calcium-dependent translocation of transcription factor ure3-bp to the plasma membrane of entamoeba histolytica. | the entamoeba histolytica upstream regulatory element 3-binding protein (ure3-bp) is a transcription factor that binds dna in a ca(2+)-inhibitable manner. the protein is located in both the nucleus and the cytoplasm but has also been found to be enriched in the plasma membrane of amebic trophozoites. we investigated the reason for the unusual localization of ure3-bp at the amebic plasma membrane. here we identify and characterize a 22-kda ca(2+)-dependent binding partner of ure3-bp, ehc2a, a nov ... | 2010 | 20023071 |
| trna gene sequences are required for transcriptional silencing in entamoeba histolytica. | transcriptional silencing by trans inactivation can contribute to the regulation of gene expression in eukaryotic cells. in the human intestinal protozoan parasite entamoeba histolytica, trans inactivation of the amoebapore-a gene (ap-a) was recently achieved by episomal transfection of e. histolytica trophozoites with the plasmid psap1. the mechanism of ap-a trans inactivation is largely unknown, though it was suggested that a partial short interspersed transposable element (sine) is required. ... | 2010 | 20023072 |
| entamoeba histolytica: a unicellular organism containing two active genes encoding for members of the tbp family. | entamoeba histolytica is the protozoan parasite which causes human amoebiasis. in this parasite, few encoding genes for transcription factors have been cloned and characterized. the e. histolytica tata-box binding protein (ehtbp) is the first basal transcription factor that has been studied. to continue with the identification of other members of the basal transcription machinery, we performed an in silico analysis of the e. histolytica genome and found three loci encoding for polypeptides with ... | 2010 | 20026212 |
| use of bacterially expressed dsrna to downregulate entamoeba histolytica gene expression. | modern rna interference (rnai) methodologies using small interfering rna (sirna) oligonucleotide duplexes or episomally synthesized hairpin rna are valuable tools for the analysis of gene function in the protozoan parasite entamoeba histolytica. however, these approaches still require time-consuming procedures including transfection and drug selection, or costly synthetic molecules. | 2009 | 20037645 |
| epithelial induction of porcine suppressor of cytokine signaling 2 (socs2) gene expression in response to entamoeba histolytica. | suppressor of cytokine signaling (socs) proteins are key physiological regulators of both innate and adaptive immunity. these proteins belong to the three major classes of modulators of cytokines signaling. in the following article, we used porcine polarized intestinal cells to study early response to the protozoan, entamoeba histolytica, and we identified by rapid amplification of cdna ends (race) pcr porcine socs1, socs4, socs5 and socs6 encoding sequences. with more than 92% identity predicte ... | 2010 | 20045027 |
| entamoeba histolytica: identification and partial characterization of alpha-mannosidase activity. | despite their well recognized importance in pathogenesis of entamoeba histolytica there are few studies dealing with the assembly and secretion of glycoproteins that participate in the adhesion to target cells and in the dissemination of the parasite in infected tissues. some of these studies refer to the identification and, in some cases, the characterization of glycosyl transferases and glycosidases involved in the biosynthesis of these macromolecules as well as to compartments involved in the ... | 2010 | 20045693 |
| [overdiagnosis of amebiasis in children with dysentery]. | there are morphologically identical amebaes, but with differences that can distinguish them; one as pathogenic: entamoeba histolytica, and the other: entamoeba dispar, as inoffensive. that brought the new hypothesis that many of the cases treated as amebiasis, weren't so. | 2009 | 20049395 |
| hydrogen peroxide-induced apoptosis-like cell death in entamoeba histolytica. | the microaerophilic intestinal parasitic protozoan entamoeba histolytica has been previously shown to be highly susceptible to oxidative stress induced by hydrogen peroxide. however the mechanism of cell death was not investigated. studies presented in this paper demonstrate several morphological features in the parasite when exposed to h(2)o(2) which are identical to metazoan apoptotic phenotype indicating a possible apoptosis-like cell death exhibited by e. histolytica in response to h(2)o(2) ... | 2010 | 20079879 |
| members of the entamoeba histolytica transmembrane kinase family play non-redundant roles in growth and phagocytosis. | entamoeba histolytica contains a large and novel family of transmembrane kinases (tmks). the expression patterns of the e. histolytica tmks in individual trophozoites and the roles of the tmks for sensing and responding to extracellular cues were incompletely characterised. here we provide evidence that single cells express multiple tmks and that tmk39 and tmk54 likely serve non-redundant cellular functions. laser-capture microdissection was used in conjunction with microarray analysis to demons ... | 2010 | 20083116 |
| acetate formation in the energy metabolism of parasitic helminths and protists. | formation and excretion of acetate as a metabolic end product of energy metabolism occurs in many protist and helminth parasites, such as the parasitic helminths fasciola hepatica, haemonchus contortus and ascaris suum, and the protist parasites, giardia lamblia, entamoeba histolytica, trichomonas vaginalis as well as trypanosoma and leishmania spp. in all of these parasites acetate is a main end product of their energy metabolism, whereas acetate formation does not occur in their mammalian host ... | 2010 | 20085767 |
| freezing of stool samples improves real-time pcr detection of entamoeba dispar and entamoeba histolytica. | pcr as a tool for intestinal parasite diagnosis is expanding since differentiation between entamoeba histolytica and entamoeba dispar cysts is impossible with microscopy. since pre-analytical factors influence dna detection, we evaluated with real-time pcr the influence of storage time and temperature. we demonstrated an improved dna detection in frozen stool samples. | 2010 | 20085792 |
| the nf-kappab p50 subunit is protective during intestinal entamoeba histolytica infection of 129 and c57bl/6 mice. | entamoeba histolytica is the agent of amebic colitis. in this work we examined the intestinal nf-kappab response to this parasite. using an enzyme-linked immunosorbent assay (elisa) and an electrophoretic mobility shift assay, we found that the nf-kappab subunit p50 predominated in nuclear extracts of whole cecal tissue and of isolated crypts from mice inoculated with e. histolytica. p50 was protective, since c57bl/6 and 129 mice in which there was targeted deletion of this subunit were more sus ... | 2010 | 20086086 |
| prevalence and spatial distribution of entamoeba histolytica/dispar and giardia lamblia among schoolchildren in agboville area (côte d'ivoire). | new efforts are being made to improve understanding of the epidemiology of the helminths and intensifying the control efforts against these parasites. in contrast, relatively few studies are being carried out in this direction for the intestinal protozoa. to contribute to a better comprehension of the epidemiology of the intestinal protozoa, prevalence, and spatial distribution of entamoeba histolytica/dispar and giardia lamblia, and their association with drinking water supplies, were determine ... | 2010 | 20087416 |
| epithelial cell apoptosis facilitates entamoeba histolytica infection in the gut. | entamoeba histolytica is the protozoan parasite that causes amebic colitis. the parasite triggers apoptosis on contact with host cells; however, the biological significance of this event during intestinal infection is unclear. we examined the role of apoptosis in a mouse model of intestinal amebiasis. histopathology revealed that abundant epithelial cell apoptosis occurred in the vicinity of amoeba in histological specimens. epithelial cell apoptosis occurred rapidly on co-culture with amoeba in ... | 2010 | 20093500 |
| prevalence of intestinal parasitic and bacterial pathogens in diarrhoeal and non-diarroeal human stools from vhembe district, south africa. | in the present study, a cross-sectional survey of intestinal parasitic and bacterial infections in relation to diarrhoea in vhembe district and the antimicrobial susceptibility profiles of isolated bacterial pathogens was conducted. stool samples were collected from 528 patients attending major public hospitals and 295 children attending two public primary schools and were analyzed by standard microbiological and parasitological techniques. entamoeba histolytica/e. dispar (34.2%) and cryptospori ... | 2009 | 20099757 |
| [intestinal parasitoses detected in primary schoolchildren in the van province]. | this study was carried out on school children in primary schools in the van province in order to investigate the distribution of intestinal parasitoses. the study was conducted in 7-15 year-old primary schoolchildren during the years from 2003-2005. stool specimens were taken from 2975 school children; of these, 1289 were female and 1686, male. the specimens were examined by native-lugol, flotation and trichrome staining methods in the parasitology laboratory of the health research and training ... | 2009 | 20101580 |
| [intestinal parasites detected in süphan primary schoolchildren in van]. | this study was performed in order to determine the distribution of intestinal parasites among students of süphan primary school in van in 2005. in the study, stool specimens were taken from 395 students (166 girls and 229 boys) between 7-15 years of age. the specimens were examined by native-lugol, flotation and trichrome staining methods in the parasitology laboratory of health research and training hospital, yüzüncü yil university. one or more parasite species were detected in 28.9 % of the ch ... | 2009 | 20101581 |
| biochemical and structural properties of chimeras constructed by exchange of cofactor-binding domains in alcohol dehydrogenases from thermophilic and mesophilic microorganisms. | the cofactor-binding domains (residues 153-295) of the alcohol dehydrogenases from the thermophile thermoanaerobacter brockii (tbadh), the mesophilic bacterium clostridium beijerinckii (cbadh), and the protozoan parasite entamoeba histolytica (ehadh1) have been exchanged. three chimeras have been constructed. in the first chimera, the cofactor-binding domain of thermophilic tbadh was replaced with the cofactor-binding domain of its mesophilic counterpart cbadh [chimera chi21((tct))]. this domain ... | 2010 | 20102159 |
| differences in the transcriptome signatures of two genetically related entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties. | the availability of two genetically very similar cell lines (a and b) derived from the laboratory isolate entamoeba histolytica hm-1:imss, which differ in their virulence properties, provides a powerful tool for identifying pathogenicity factors of the causative agent of human amoebiasis. cell line a is incapable inducing liver abscesses in gerbils, whereas interaction with cell line b leads to considerable abscess formation. phenotypic characterization of both cell lines revealed that trophozoi ... | 2010 | 20102605 |
| vanadium complexes having [v(iv)o](2+) and [v(v)o(2)](+) cores with binucleating dibasic tetradentate ligands: synthesis, characterization, catalytic and antiamoebic activities. | binucleating hydrazones ch(2)(h(2)sal-bhz)(2) (i) and ch(2)(h(2)sal-fah)(2) (ii), derived from 5,5'-methylbis(salicylaldehyde) and benzoylhydrazide or 2-furoylhydrazide, react with [v(iv)o(acac)(2)] to give dinuclear v(iv)o-complexes [ch(2){v(iv)o(sal-bhz)(h(2)o)}(2)] 1 and [ch(2){v(iv)o(sal-fah)(h(2)o)}(2)] 4, respectively. in the presence of koh or csoh.h(2)o, oxidation of 1 and 2 results in the formation of dioxidovanadium(v) complexes, k(2)[ch(2){v(v)o(2)(sal-bhz)}(2)].2h(2)o 2, k(2)[ch(2){v ... | 2010 | 20104362 |
| new dioxazole derivatives: synthesis and effects on the growth of entamoeba histolytica and giardia intestinalis. | cyclization of oxime with different aldehydes and ketones under basic condition led to the formation of new dioxazole derivatives and the structure was elucidated by spectral data. the effects of diaoxazoles on the inhibition of growth of entamoeba histolytica and giardia intestinalis in vitro have been determined, and selected compounds further investigated for their toxicity. sar showed that the compounds with 5-nitrothiophene group at the 3-postion of the diaoxazole ring were more active than ... | 2010 | 20122767 |
| th-1/th-2 cytokine pattern in human amoebic colitis. | amoebiasis, caused by entamoeba histolytica, is still considered a major health problem in developing countries. since the immune response during human amoebiasis has not been clearly defined, we chose to evaluate cytokine production in patients suffering from amoebic colitis. a case-control association study was carried out on 62 subjects, including 31 patients with amoebic colitis and 31 healthy controls (age, sex and geographic region-matched). serum levels of il-12, ifn-gamma, il-13 and il-5 ... | 2009 | 20128506 |
| application of real-time polymerase chain reaction in detection of entamoeba histolytica in pus aspirates of liver abscess patients. | entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. recently, dna molecular-based methods have been employed to enhance the detection of e. histolytica in either pus or stool specimens. in this study, the results of real-time polymerase chain reaction (pcr) to detect e. histolytica dna in pus from liver abscess cases were compared with those of indirect hemagglutination assay on the corresponding serum samples. bacterial cultu ... | 2010 | 20132028 |
| entamoeba histolytica: expression and localization of gal/galnac lectin in virulent and non-virulent variants from hm1:imss strain. | we have purified gal/galnac lectin from entamoeba histolytica by electroelution. the purified protein was used to immunize rabbits and obtain polyclonal igg's anti-lectin. these antibodies were used as tools to analyze the expression and localization of the amoebic lectin in both virulent (veh) and non-virulent (nveh) variants of axenically cultured hm1:imss strain. veh is able to induce liver abscesses in hamsters, whereas nveh has lost this ability. in vitro, amoebic trophozoites from both var ... | 2010 | 20138867 |
| entamoeba histolytica: molecular cloning and characterization of a novel neutral sphingomyelinase. | a novel neutral sphingomyelinase (nsmase) was characterized in entamoeba histolytica trophozoites. smase, a sphingomyelin-specific form of phospholipase c, catalyzes the hydrolysis of sphingomyelin to ceramide and phosphorylcholine. three amebic putative nsmase genes were found to be actively transcribed. mg(2+)-independent nsmase activity in the soluble fraction of the trophozoites was stimulated by mn(2+) and partially inhibited by zn(2+). nsmase activity of the recombinant protein ehnsm1, inc ... | 2010 | 20138872 |
| effect of the monocyte locomotion inhibitory factor (mlif) produced by e. histolityca on cytokines and chemokine receptors in t cd4+ lymphocytes. | entamoeba histolytica produces monocyte locomotion inhibitory factor (mlif), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. leukocytes are affected through the modulation of cytokine expression and/or production. we evaluated the effects of mlif on the activation and production of intracellular cytokines in human cd4+ t lymphocytes by flow cytometry. cells were stimulated for 24 h with pma, mlif, or pma+mlif. cellular activation was measured using anti-cd ... | 2010 | 20140297 |
| microbicidal effect of the lactoferrin peptides lactoferricin17-30, lactoferrampin265-284, and lactoferrin chimera on the parasite entamoeba histolytica. | entamoeba histolytica is a parasitic protozoan that produces amoebiasis, an intestinal disease characterized by ulcerative colitis and dysentery. in some cases, trophozoites can travel to the liver leading to hepatic abscesses and death. recently, lactoferrin and lactoferricin b have been shown to be amoebicidal in axenic cultures. the aim of this work was to determine whether the lactoferrin-peptides lactoferricin amino acids 17-30, lactoferrampin amino acids 265-284, and lactoferrin chimera wh ... | 2010 | 20140481 |
| intestinal protozoa infections among patients with ulcerative colitis: prevalence and impact on clinical disease course. | epidemiological and microbiologic studies suggest that enteropathogenic microorganisms play a substantial role in the clinical initiation and relapses of inflammatory bowel disease. | 2010 | 20145404 |
| the role of lipopeptidophosphoglycan in the immune response to entamoeba histolytica. | the sensing of pathogen associated molecular patterns (pamps) by innate immune receptors, such as toll-like receptors (tlrs), is the first step in the inflammatory response to pathogens. entamoeba histolytica, the etiological agent of amebiasis, has a surface molecule with the characteristics of a pamp. this molecule, which was termed lipopeptidophosphoglycan (lppg), is recognized through tlr2 and tlr4 and leads to the release of cytokines from human monocytes, macrophages, and dendritic cells; ... | 2010 | 20145703 |
| amoebiasis cutis: clinical suspicion is the key to early diagnosis. | amoebiasis cutis is a rare manifestation of entamoeba histolytica, primarily an intestinal pathogen, which occurs as a complication of amoebic dysentery. primary cutaneous amoebiasis occurs from contamination of pre-existing wounds. a high degree of clinical suspicion and demonstration of trophozoites from lesions are important for making an early diagnosis lest these patients should suffer significant morbidity. a hiv-negative and otherwise healthy 40-year-old man presented with a well-defined, ... | 2010 | 20148845 |
| entamoeba histolytica sirtuin ehsir2a deacetylates tubulin and regulates the number of microtubular assemblies during the cell cycle. | we have discovered four sirtuin genes in entamoeba histolytica, two of which are similar to eukaryotic sirtuins and two to bacterial and archaeal sirtuins. the eukaryotic sirtuin homologue, ehsir2a, showed nad(+)-dependent deacetylase activity and was sensitive to class iii hdac inhibitors. localization of ehsir2a at different cellular sites suggested that this deacetylase could have multiple targets. using an e. histolytica cdna library in the yeast two-hybrid genetic screen, we identified seve ... | 2010 | 20148900 |
| u12 type introns were lost at multiple occasions during evolution. | two categories of introns are known, a common u2 type and a rare u12 type. these two types of introns are removed by distinct spliceosomes. the phylogenetic distribution of spliceosomal rnas that are characteristic of the u12 spliceosome, i.e. the u11, u12, u4atac and u6atac rnas, suggest that u12 spliceosomes were lost in many phylogenetic groups. we have now examined the distribution of u2 and u12 introns in many of these groups. | 2010 | 20149226 |
| seroprevalence of entamoeba histolytica in the context of hiv and aids: the case of vhembe district, in south africa's limpopo province. | in a recent study in northern south africa, the seroprevalence of entamoeba histolytica infection among 257 hiv-positive and 117 hiv-negative individuals was determined, using an elisa for the detection of antibodies reacting with the parasite's galactose/-acetyl-d-galactosamine(gal/galnac)-inhibitable adherence lectin. overall, 34.0% of the 374 participants (36.1% of the females and 28.1% of the males) were found seropositive for e. histolytica. although all age-groups were affected by the amoe ... | 2010 | 20149292 |
| the r2r3 myb protein family in entamoeba histolytica. | the myb dna-binding domain is conserved in vertebrates, plants, and fungi. this domain mediates the dna-binding activity of proteins (that have transcription factor activity) in a sequence-specific manner and is also used for the protection of telomeric regions. the myb dna-binding domain contains three imperfect conserved repeats of 52 amino acids (r1, r2, and r3). within each repeat, there are three tryptophans that are separated by 18 or 19 amino acids. in order to understand the role of myb ... | 2010 | 20156532 |
| in vitro induction of entamoeba histolytica cyst-like structures from trophozoites. | inhibition of encystment can be conceived as a potentially useful mechanism to block the transmission of entamoeba histolytica under natural conditions. unfortunately, amoeba encystment has not been achieved in vitro and drugs inhibiting the formation of cysts are not available. luminal conditions inducing encystment in vivo are also unknown, but cellular stress such as exposure to reactive oxygen species from immune cells or intestinal microbiota could be involved. a role for certain divalent c ... | 2010 | 20169067 |
| differential identification of entamoeba spp. based on the analysis of 18s rrna. | entamoeba histolytica is known to cause intestinal and extra-intestinal disease while the other entamoeba species are not considered to be pathogenic. however, all entamoeba spp. should be reported when identified in clinical samples. entamoeba polecki, entamoeba coli, and entamoeba hartmanii can be differentiated morphologically from e. histolytica, but some of their diagnostic morphologic features overlap. e. histolytica, entamoeba dispar, and entamoeba moshkovskii are morphologically identica ... | 2010 | 20169364 |
| a new nuclear function of the entamoeba histolytica glycolytic enzyme enolase: the metabolic regulation of cytosine-5 methyltransferase 2 (dnmt2) activity. | cytosine-5 methyltransferases of the dnmt2 family function as dna and trna methyltransferases. insight into the role and biological significance of dnmt2 is greatly hampered by a lack of knowledge about its protein interactions. in this report, we address the subject of protein interaction by identifying enolase through a yeast two-hybrid screen as a dnmt2-binding protein. enolase, which is known to catalyze the conversion of 2-phosphoglycerate (2-pg) to phosphoenolpyruvate (pep), was shown to h ... | 2010 | 20174608 |
| dyspnea and dysentery: a case report of pleuropulmonary amebiasis. | pleuropulmonary amebiasis is an uncommon complication of entamoeba histolytica infection. it typically occurs in endemic regions including central and south america, africa and the indian subcontinent. the case of a previously healthy us army male stationed in liberia with an acute onset of fevers, abdominal pain and bloody diarrhea is presented. he developed a productive cough with pleuritic chest pain and imaging revealed multiple liver abscesses, with rupture through the diaphragm causing a l ... | 2010 | 20175253 |
| entamoeba histolytica phosphoserine aminotransferase (ehpsat): insights into the structure-function relationship. | abstract: | 2010 | 20199659 |
| epizootic fatal amebiasis in an outdoor group of old world monkeys. | entamoeba (e.) histolytica is an obligate parasite of humans and non-human primates. | 2010 | 20202078 |
| regulation of gene expression in protozoa parasites. | infections with protozoa parasites are associated with high burdens of morbidity and mortality across the developing world. despite extensive efforts to control the transmission of these parasites, the spread of populations resistant to drugs and the lack of effective vaccines against them contribute to their persistence as major public health problems. parasites should perform a strict control on the expression of genes involved in their pathogenicity, differentiation, immune evasion, or drug r ... | 2010 | 20204171 |
| studies on the culture of endamoeba histolytica. | | 1947 | 20243110 |
| the part played by the faeces and vomit-drop in the transmission of entamoeba histolytica by musca domestica. | | 1947 | 20249297 |
| observations on the excystation of endamoeba histolytica. | | 1947 | 20256993 |
| [not available]. | | 1947 | 20261137 |
| studies on amoebiasis; a clinical study of children passing cysts of entamoeba histolytica. | | 1947 | 20264805 |
| survival time of entamoeba histolytica in feces. | | 1947 | 20264866 |
| endamoeba histolytiça (schaudinn 1903) hickson 1909. | | 1946 | 20273614 |
| the significance of the small varieties of endamoeba histolytica. | | 1945 | 20275605 |
| differences in culturability, infectivity and pathogenicity of human strains of endamoeba histolytica. | | 1946 | 20277720 |
| biological studies on endamoeba histolytica; the growth cycle of populations in a mixed bacterial flora. | | 1946 | 20279491 |
| cultivation of endamoeba histolytica in artificial media from cysts in drinking water subjected to chlorination. | | 1946 | 20279492 |
| [improvement of the index of prevalence of entamoeba histolytica]. | | 1945 | 20280607 |
| in vitro effect of penicillin on endamoeba histolytica. | | 1946 | 20282133 |
| agglutination of endamoeba histolytica cysts. | | 1947 | 20292221 |
| the rate of multiplication of endamoeba histolytica and its relation to in vitro drug testing and possibly to nutritional studies. | | 1947 | 20294090 |
| a 23-year old male with cough, fever and blood-streaked sputum. | | 2009 | 20298388 |
| hydrogen peroxide induces apoptosis-like death in entamoeba histolytica trophozoites. | programmed cell death (pcd) is an essential process in the growth and development of multicellular organisms. however, accumulating evidence indicates that unicellular eukaryotes can also undergo pcd with apoptosis-like features. this study demonstrates that after exposure to 0.8 mm h(2)o(2) for 9 h entamoeba histolytica presents morphological and biochemical evidence of apoptosis-like death. morphological characteristics of apoptosis-like death including dna fragmentation, increased vacuolizati ... | 2010 | 20299403 |
| identification of four entamoeba histolytica organellar dna polymerases of the family b and cellular localization of the ehodp1 gene and ehodp1 protein. | we report the identification of a family of four active genes (ehodp1, ehodp2, ehodp3, and ehodp4) encoding putative dna polymerases in entamoeba histolytica, the protozoan parasite responsible of human amoebiasis. the four ehodp genes show similarity to dna polymerases encoded in fungi and plant mitochondrial plasmids. ehodp polypeptides conserve the 3'-5' exonuclease ii and 5'-3' polymerization domains, and they have the i, ii, and iii conserved boxes that characterize them as dna polymerases ... | 2010 | 20300437 |
| protection against murine intestinal amoebiasis induced by oral immunization with the 29 kda antigen of entamoeba histolytica and cholera toxin. | entamoeba histolytica antigens recognized by salivary iga from infected patients include the 29 kda antigen (eh29), an alkyl hydroperoxide reductase. here, we investigate the potential of recombinant eh29 and an eh29-cholera toxin subunit b (ctxb) fusion protein to confer protection against intestinal amoebiasis after oral immunization. the purified eh29-ctxb fusion retained the critical ability to bind ganglioside gm(1), as determined by elisa. oral immunization of c3h/hej mice with eh29 admini ... | 2010 | 20303954 |
| the immunopathogenesis of entamoeba histolytica. | amebiasis is the disease caused by the enteric dwelling protozoan parasite entamoeba histolytica. the who considers amebiasis as one of the major health problems in developing countries; it is surpassed by only malaria and schistosomiasis for death caused by parasitic infection. e. histolytica primarily lives in the colon as a harmless commensal, but is capable of causing devastating dysentery, colitis and liver abscess. what triggers the switch to a pathogenic phenotype and the onset of disease ... | 2010 | 20303955 |
| diagnosis of entamoeba histolytica in symptomatic children, jeddah city, saudi arabia. | amebiasis is one of the most common parasitic infections worldwide. entamoeba histolytica and entamoeba dispar are two morphologically indistinguishable human protozoa parasites that are genetically distinct species. differential diagnosis of e. histolytica (pathogenic) and e. dispar (non-pathogenic) is essential both for treatment decision and public health knowledge. stool samples from 500 randomly selected children complaining of gastroenteritis were examined microscopically by direct wet sme ... | 2008 | 20306672 |
| intestinal parasitosis in school going children of dharan municipality, nepal. | this community-based investigation was carried out to estimate the prevalence of intestinal parasitosis in primary school-going children of the dharan municipality, sunsari, nepal from 2007 through 2008. a total of 182 stool samples were collected from 101 male and 81 female children of ages 4 to 10 years. a questionnaire including the factors predisposing to parasitosis, was filled up by the parents of each child. parasitic examination was carried out by direct wet mount examination and concent ... | 2009 | 20306743 |
| remarks on intestinal parasites in montreal, and the relation of entamoeba histolytica to colitis. | | 1934 | 20319387 |
| entamoeba histolytica and colitis in montreal. | | 1934 | 20319388 |
| entamoeba histolytica erratum. | | 1934 | 20319444 |
| entamoeba histolytica. | | 1934 | 20319452 |
| study of children passing e. histolytica: a clinical study of children passing cysts of entamoeba histolytica. | | 1947 | 20324277 |
| discrimination of entamoeba moshkovskii in patients with gastrointestinal disorders by single-round pcr. | entamoeba moshkovskii and entamoeba dispar are impossible to differentiate microscopically from the pathogenic species entamoeba histolytica. there are limited data on the prevalence of these commensal parasites in iran. we utilized a single-round pcr assay to determine the prevalence of e. moshkovskii, e. dispar, and e. histolytica in stool samples from iranian patients infected with gastrointestinal disorders. after culturing of microscopy-positive isolates and extraction of dna, pcr was carri ... | 2010 | 20332579 |
| the essentials of protein import in the degenerate mitochondrion of entamoeba histolytica. | several essential biochemical processes are situated in mitochondria. the metabolic transformation of mitochondria in distinct lineages of eukaryotes created proteomes ranging from thousands of proteins to what appear to be a much simpler scenario. in the case of entamoeba histolytica, tiny mitochondria known as mitosomes have undergone extreme reduction. only recently a single complete metabolic pathway of sulfate activation has been identified in these organelles. the e. histolytica mitosomes ... | 2010 | 20333239 |
| entamoeba histolytica and e. dispar trophozoites in the liver of hamsters: in vivo binding of antibodies and complement. | abstract: | 2010 | 20338063 |
| ehncabp166: a nucleocytoplasmic actin-binding protein from entamoeba histolytica. | the actin cytoskeleton consists of multiple actin binding proteins (abps) that participate cooperatively in different cellular functions such as the maintenance of polarity and cell motility as well as the invasion of target cells and regulation of gene expression, among others. due to the important role of abps in the pathogenesis of entamoeba histolytica, the role of a new nucleocytoplasmic abp from e. histolytica named ehncabp166 was investigated. the ehncabp166 gene encodes a protein with an ... | 2010 | 20338197 |
| [the distribution of intestinal parasites detected in the dokuz eylul university medical faculty hospital between 2005 and 2008.] | a retrospective evaluation of the data from 14,246 patients with gastrointestinal complaints who presented at the parasitology laboratory of the dokuz eylul university medical faculty hospital between january 2005 and december 2008 was carried out. fecal samples of all patients were examined using native-lugol and the trichrome and kinyoun acid-fast staining method after sedimentation in fecal concentration tubes. one or more parasites were detected in 1320 (9.3%) of the patients. the distributi ... | 2010 | 20340084 |
| association between tnf-alpha and entamoeba histolytica diarrhea. | an association between tumor necrosis factor alpha (tnf-alpha) and entamoeba histolytica diarrhea was assessed in a cohort of 138 non-related bangladeshi children who have been prospectively followed since 2001. peripheral blood mononuclear cells (pbmcs) obtained at study entry were purified, cultured, and stimulated with soluble amebic antigen before cytokine measurement from supernatant. higher levels of tnf-alpha were associated with increased risk of first (p = 0.01) and recurrent e. histoly ... | 2010 | 20348510 |
| entamoeba histolytica: soluble and membrane-associated neutral sphingomyelinase-c and other unidentified esterase activity. | sphingomyelinase (smase) activity was measured in entamoeba histolytica particulate and soluble subcellular fractions. the effects on smase of incubation time, total protein concentration, ph, and several divalent cations were determined. smase-c and other unidentified esterase activity were detected in soluble and particulate fractions. smase-c was 94.5-96.0% higher than the unidentified esterase activity. soluble and insoluble smase-c specific activities increased with protein dose and incubat ... | 2010 | 20350542 |
| parasitic contamination in wastewater and sludge samples in tunisia using three different detection techniques. | the limited availability of water results in the reuse of wastewater or sludge. the tunisian wastewater regulatory guidelines have specific limits for ova of helminths (<1 egg/l) but none for protozoan parasites. we assessed the presence and loads of parasites in 20 samples of raw, treated wastewater and sludge collected from six wastewater treatment plants. samples were tested by microscopy using the modified bailenger method (mbm), immunomagnetic separation (ims) followed by immunofluorescent ... | 2010 | 20352447 |
| urinary hyaluronidase activity in rats infected with blastocystis hominis--evidence for invasion? | the fact whether blastocystis hominis can invade has always been in question. apart from a few sporadic studies such as that done on gnotobiotic guinea pigs which showed surface invasion and mucosal inflammation of the host's intestine caused by b. hominis infection, no real documentation of invasion has been proven. studies have shown that hyaluronidase is secreted during the penetration into the host's skin and gut by nematode parasites. hyaluronidase activity in protozoa namely entamoeba hist ... | 2010 | 20358228 |
| intestinal polyparasitism in a rural kenyan community. | polyparasitism seems to be a common feature in human populations in sub-saharan africa. however, very little is known about its epidemiological significance, its long term impact on human health or the types of interactions that occur between the different parasite species involved. | 2009 | 20358789 |
| pyogenic versus amoebic liver abscesses. a comparative clinical study in a series of 58 patients. | to compare the clinical and epidemiological characteristics of patients with pyogenic liver abscess (pla) and with amebic liver abscess (aha) in order to determine the potential factors that may help improve diagnosis and treatment for this disease. | 2010 | 20361845 |
| intestinal parasitic infections among intellectual disability children in rehabilitation centers of northern iran. | parasitic infection is highly prevalent throughout the developing countries of the world. these infections are the major problem in rehabilitation centers for the mentally retarded. there have been many reports about the prevalence of parasitic infection among different groups of people in iran; however, the epidemiological data in intellectual disability (id) individuals are rare and there are no such data from northern iran. the aim of the present study was to determine the incidence of intest ... | 2010 | 20363588 |
| a novel entamoeba histolytica cysteine proteinase, ehcp4, is key for invasive amebiasis and a therapeutic target. | entamoeba histolytica cysteine proteinases (ehcps) play a key role in disrupting the colonic epithelial barrier and the innate host immune response during invasion of e. histolytica, the protozoan cause of human amebiasis. ehcps are encoded by 50 genes, of which ehcp4 (ehcp-a4) is the most up-regulated during invasion and colonization in a mouse cecal model of amebiasis. up-regulation of ehcp4 in vivo correlated with our finding that co-culture of e. histolytica trophozoites with mucin-producing ... | 2010 | 20378535 |
| localization and targeting of an unusual pyridine nucleotide transhydrogenase in entamoeba histolytica. | pyridine nucleotide transhydrogenase (pnt) catalyzes the direct transfer of a hydride-ion equivalent between nad(h) and nadp(h) in bacteria and the mitochondria of eukaryotes. pnt was previously postulated to be localized to the highly divergent mitochondrion-related organelle, the mitosome, in the anaerobic/microaerophilic protozoan parasite entamoeba histolytica based on the potential mitochondrion-targeting signal. however, our previous proteomic study of isolated phagosomes suggested that pn ... | 2010 | 20382757 |
| calpain-dependent cleavage of shp-1 and shp-2 is involved in the dephosphorylation of jurkat t cells induced by entamoeba histolytica. | host cell death induced by entamoeba histolytica is an important mechanism for both host defence and microbial immune evasion during human amoebiasis. however, the signalling pathways underlying cell death induced by e. histolytica are not fully understood. this study investigated the involvement of the protein tyrosine phosphatases (ptps) shp-1 and shp-2 in the dephosphorylation associated with e. histolytica-induced host cell death. incubation with e. histolytica resulted in a marked decrease ... | 2010 | 20398180 |
| parasiticidal effect of 16alpha-bromoepiandrosterone (epibr) in amoebiasis and cysticercosis. | the effect of the dehydroepiandrosterone analog 16alpha-bromoepiandrosterone (epibr) was tested on the tapeworm taenia crassiceps and the protist entamoeba histolytica, both in vivo and in vitro. administration of epibr prior to infection with cysticerci in mice reduced the parasite load by 50% compared with controls. epibr treatment induced 20% reduction on the development of amoebic liver abscesses in hamsters. in vitro treatment of t. crassiceps and e. histolytica cultures with epibr, reduced ... | 2010 | 20403456 |