| [epidemiological retrospective survey intestinal parasitism in the provincial hospital center (kenitra, morocco): review of 10 years (1996-2005)]. | the survey drew up the epidemiological situation of intestinal parasitism in the center of health el idrissi (kenitra, morocco). the number of reviews has decreased between 1996 and 2005. a correlation between the number of examinations and years of the study period was observed (p <0.001). 4285 stool specimens collected in 1996-2005 were tested by parasitologic examination. among the persons examined, 606 of them were parasited by one or several species, say an infestation index of 14.15%. amoe ... | 2009 | 19297294 |
| development of an immunobinding dot-blot assay as an alternative for the serodiagnosis of human cysticercosis. | an immunobinding dot-blot assay (dba) was developed on nitrocellulose paper for the serodiagnosis of human cysticercosis, using cysticercus cellulosae as antigen. the dba had an immunological sensitivity of 0.08 mg of antigen protein/ml; however, it showed cross-reactions with antigens of adult taenia solium and echinococcus granulosus, but not with toxoplasma gondii and entamoeba histolytica antigens. an enzyme-linked immunosorbent assay (elisa) was used as the gold standard for obtaining the d ... | 2009 | 19317923 |
| development of loop-mediated isothermal amplification assay for detection of entamoeba histolytica. | a novel one-step, closed-tube, loop-mediated isothermal amplification (lamp) assay for detecting entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. the sensitivity of the lamp assay is 1 parasite per reaction. a total of 130 clinical samples were analyzed, and the results compared with those of conventional nested pcr to validate the practicability of this assay. no dna was amplified from other diarrheal pathogens, such as other entamoeba specie ... | 2009 | 19321720 |
| prospective case-control study of the association between common enteric protozoal parasites and diarrhea in bangladesh. | the parasitic causes of diarrhea have historically been identified by use of microscopy; however, the use of this technique does not allow one to distinguish between subspecies or genotypes of parasites. our objective was to determine, by use of modern diagnostic methods, the proportion of diarrhea cases in bangladesh attributable to cryptosporidium hominis, cryptosporidium parvum, entamoeba histolytica, and giardia lamblia assemblages a and b. | 2009 | 19323634 |
| ameboma of the colon with amebic liver abscess mimicking metastatic colon cancer. | amebic colitis is common in developing countries, with its variable and non-specific symptoms. amebomas occur rarely, resulting from the formation of annular granulation tissue, usually in the cecum and in the ascending colon. this report describes the case of a 59-year-old male who presented with abdominal pain. radiological examination depicted concentric thickening of the cecal wall with mass formation and a cystic lesion in the liver. the endoscopy performed showed a growth in the ascending ... | 2009 | 19332922 |
| presence of parasitic protozoa and helminth in sewage and efficiency of sewage treatment in tunisia. | helminth eggs and protozoan cysts were enumerated in raw and treated wastewater in tunisia in order to determine their removal by wastewater treatment and to provide quantitative data for developing regulations for wastewater quality that are currently lacking. raw and treated wastewater samples were collected from 17 plants in tunisia during 2006-2007 and analyzed for parasites using the modified bailenger method. two groups of parasites, namely, ascaris sp., entamoeba coli, enterobius vermicul ... | 2009 | 19333622 |
| inter-cellular variation in dna content of entamoeba histolytica originates from temporal and spatial uncoupling of cytokinesis from the nuclear cycle. | accumulation of multiple copies of the genome in a single nucleus and several nuclei in a single cell has previously been noted in entamoeba histolytica, contributing to the genetic heterogeneity of this unicellular eukaryote. in this study, we demonstrate that this genetic heterogeneity is an inherent feature of the cell cycle of this organism. chromosome segregation occurs on a variety of novel microtubular assemblies including multi-polar spindles. cytokinesis in e. histolytica is completed b ... | 2009 | 19352422 |
| targeting histone deacetylase inhibitors for anti-malarial therapy. | it is now clear that histone acetylation plays key roles in regulating gene transcription in both eukaryotes and prokaryotes, the acetylated form inducing gene expression while deacetylation silences genes. recent studies have identified roles for histone acetyltransferases (hats) and/or histone deacetylases (hdacs) in a number of parasites including entamoeba histolytica, toxoplasma gondii, schistosoma mansoni, cryptosporidium sp., leishmania donovani, neospora caninum, and plasmodium falciparu ... | 2009 | 19355992 |
| oxidative folding and reductive activities of ehpdi, a protein disulfide isomerase from entamoeba histolytica. | pdi enzymes are oxidoreductases that catalyze oxidation, reduction and isomerization of disulfide bonds in polypeptide substrates. we have previously identified an e. histolytica pdi enzyme (ehpdi) that exhibits oxidase activity in vivo. however, little is known about the specific role of its redox-related structural features on the enzymatic activity. here, we have studied the in vivo oxidative folding of ehpdi by mutagenic analysis and functional complementation assays as well as the in vitro ... | 2009 | 19361571 |
| proteomic comparison of entamoeba histolytica and entamoeba dispar and the role of e. histolytica alcohol dehydrogenase 3 in virulence. | the protozoan intestinal parasite entamoeba histolytica infects millions of people worldwide and is capable of causing amebic dysentery and amebic liver abscess. the closely related species entamoeba dispar colonizes many more individuals, but this organism does not induce disease. to identify molecular differences between these two organisms that may account for their differential ability to cause disease in humans, we used two-dimensional gel-based (dige) proteomic analysis to compare whole ce ... | 2009 | 19365541 |
| [detection of entamoeba histolytica in stool specimens with the elisa method]. | in this study, carried out between november 2006 and september 2007, amebic cysts were observed in 44 (0.37%) out of a total of 1720 stool specimens which were examined by direct microscopy. entamoeba histolytica specific antigen was investigated with elisa in the specimens in which cysts were observed. specific antigen was detected in 26 (59.1%) of these specimens. the diagnosis of amoebiasis for the patients whose elisa tests were positive was confirmed and the appropriate therapy was immediat ... | 2009 | 19367537 |
| [the determination of intestinal parasites, physical growth and hygiene behaviors of children in the mersin city social service child care centre]. | intestinal parasites are still a major health problem in our country. the aim of this study was the determination of intestinal parasites, physical growth and hygiene behavior of a total of 106 children living in the mersin city social service child care centre and who could participate in this research. a questionnaire form was used to determine the socio-demographic features and hygienic behavior of the children, and their weight and height were measured in order to determine their physical gr ... | 2009 | 19367550 |
| thymidine kinase and uridine-cytidine kinase from entamoeba histolytica: cloning, characterization, and search for specific inhibitors. | entamoeba histolytica is an intestinal parasite and the causative agent of amoebiasis, which is a significant source of morbidity and mortality in developing countries. although anti-amoebic drugs such as metronidazole, emetine, chloroquine and nitazoxanide are generally effective, there is always potential for development of drug resistance. in order to find novel targets to control e. histolytica proliferation we cloned, expressed and purified thymidine kinase (eh-tk) and uridine-cytidine kina ... | 2009 | 19368743 |
| antiamoebic drugs for treating amoebic colitis. | entamoeba histolytica infection is common in developing countries, and up to 100,000 individuals with severe disease die every year. adequate therapy for amoebic colitis is necessary to reduce the severity of illness, prevent development of complicated disease and extraintestinal spread, and decrease transmission. | 2009 | 19370624 |
| computational study of the electronic structure characterization of a novel anti-inflammatory tripeptide derived from monocyte locomotion inhibitory factor (mlif)-pentapeptide. | the structure and properties of molecules are determined by their charge-density distribution. several works have shown that electron delocalization along the peptide backbone and side-chain modulates the physical and chemical features of peptides and protein properties. research on entamoeba histolytica-soluble factors led to the identification of the pentapeptide met-gln-cys-asn-ser, with anti-inflammatory in vivo and in vitro effects. a synthetic pentapeptide, met-pro-cys-asn-ser, maintained ... | 2009 | 19371982 |
| molecular nature of virulence in entamoeba histolytica. | for many years virulence of pathogenic entamoeba histolytica has been attributed to the capacity of the parasite to destroy tissues through the expression and/or secretion of various molecules. such view is supported mainly by in vitro experimentation, whereas data obtained by using animal models of the disease have clearly demonstrated that the host's inflammatory response is primarily responsible for tissue damage. this review analyzes the content and/or activity of some of the presumed toxic ... | 2009 | 19376272 |
| overexpression of a mutant form of ehraba, a unique rab gtpase of entamoeba histolytica, alters endoplasmic reticulum morphology and localization of the gal/galnac adherence lectin. | entamoeba histolytica is a protozoan parasite that causes amoebic dysentery and liver abscess. vesicle trafficking events, such as phagocytosis and delivery of plasma membrane proteins, have been implicated in pathogenicity. rab gtpases are proteins whose primary function is to regulate vesicle trafficking; therefore, understanding the function of rabs in this organism may provide insight into virulence. e. histolytica possesses a number of unique rabs that exhibit limited homology to host rabs. ... | 2009 | 19377040 |
| synthesis of novel hybrid molecules from precursors with known antiparasitic activity. | three novel new compounds derived from antiparasitic precursors have been synthesized and tested for their antiamoebic and antigiardial activities. the condensation of 2-(2-methyl-5-1h-nitroimidazolyl)ethylamine (6) with 5-nitro-2-furylacrylic acid (7) gave 3-(5-nitrofuran-2-yl)-n-[2-(5-nitroimidazol-1-yl)ethyl]acrylamide (8). condensation of 7 with 7-chloro-4-(piperazin-1-yl)quinoline (9) afforded 1-[4-(7-chloroquinolin-4-yl)piperazin-1-yl)-3-(5-nitrofuran-2-yl)propenone as a mixture of two iso ... | 2009 | 19384280 |
| n- and c-terminal domains of the calcium binding protein ehcabp1 of the parasite entamoeba histolytica display distinct functions. | entamoeba histolytica, a protozoan parasite, is the causative agent of amoebiasis, and calcium signaling is thought to be involved in amoebic pathogenesis. ehcabp1, a ca(2+) binding protein of e. histolytica, is essential for parasite growth. high resolution crystal structure of ehcabp1 suggested an unusual arrangement of the ef-hand domains in the n-terminal part of the structure, while c-terminal part of the protein was not traced. the structure revealed a trimer with amino terminal domains of ... | 2009 | 19384409 |
| entamoeba histolytica: cysteine proteinase activity and virulence. focus on cysteine proteinase 5 expression levels. | cysteine proteinase (cp) activity and cp5 mrna levels were analyzed in eleven samples of entamoeba histolytica isolated from patients presenting different clinical profiles. the virulence degree of the isolates, determined in hamster liver, correlated well with the clinical form of the patient and culture conditions. cp5 mrna levels were also determined in sample freshly picked up directly from liver amoebic abscess. differences were not observed in the levels of cp5 mrna and cp specific activit ... | 2009 | 19389401 |
| pleural empyema secondary to amebic liver abscess. | | 2009 | 19389640 |
| frequency of human toxocariasis in a rural population from cajamarca, peru determined by dot-elisa test. | the aim of this study was to estimate the frequency of human toxocariasis in cauday district, cajamarca, peru, using a dot-elisa test. from june to october 2005, a total of 256 adult subjects were studied. blood samples were collected for serology by a dot-elisa test and for hematological examination. parasitological examination was also carried out in stool samples to check cross-reactions in the dot-elisa. the frequency observed was 44.92%, with a significant higher proportion of positivity in ... | 2009 | 19390733 |
| coinfections with schistosoma haematobium, necator americanus, and entamoeba histolytica/entamoeba dispar in children: chemokine and cytokine responses and changes after antiparasite treatment. | the effect of polyparasite infections on cytokine and chemokine responses as well as the effect of antiparasite treatment was studied in children without parasite infection (the g0 group), in children singly infected with schistosoma haematobium (the g1 group), and in children multiply infected with s. haematobium/schistosoma mansoni, entamoeba histolytica/entamoeba dispar, and necator americanus (the g3+ group). linear regression analysis disclosed a significant risk for coinfection with hookwo ... | 2009 | 19392635 |
| a pcdna-ehcpadh vaccine against entamoeba histolytica elicits a protective th1-like response in hamster liver. | dna vaccines are promising tools to fight parasitic diseases, including amoebiasis caused by the protozoan entamoeba histolytica. here we studied the immunogenicity and protective efficacy of a dna vaccine against this parasite composed by the ehcpadh surface complex encoding genes (ehcp112 and ehadh112). ehcpadh is formed by an adhesin (ehadh112) and a cysteine proteinase (ehcp112), both involved in the parasite virulence. we evaluated transcription, protein expression, immunological response a ... | 2009 | 19406180 |
| attribution of malnutrition to cause-specific diarrheal illness: evidence from a prospective study of preschool children in mirpur, dhaka, bangladesh. | we examined whether malnutrition (underweight [waz] < -2) increased the risk of diarrhea equally for all enteropathogens. the study was conducted prospectively between january 1999 and july 2002 in mirpur, an urban slum in dhaka. two hundred eighty-nine bangladeshi children (147 male and 142 female) 2-5 years of age were included in the study. malnutrition was present in 39% of the children at the time of enrollment. the parents and children were visited and interviewed every other day by health ... | 2009 | 19407131 |
| a case of duodenal entamoeba histolytica. | | 2009 | 19410047 |
| molecular diagnostics of intestinal parasites in returning travellers. | a new diagnostic strategy was assessed for the routine diagnosis of intestinal parasites in returning travellers and immigrants. over a period of 13 months, unpreserved stool samples, patient characteristics and clinical data were collected from those attending a travel clinic. stool samples were analysed on a daily basis by microscopic examination and antigen detection (i.e. care as usual), and compared with a weekly performed multiplex real-time polymerase chain reaction (pcr) analysis on enta ... | 2009 | 19415354 |
| trichomonas vaginalis: metronidazole and other nitroimidazole drugs are reduced by the flavin enzyme thioredoxin reductase and disrupt the cellular redox system. implications for nitroimidazole toxicity and resistance. | infections with the microaerophilic parasite trichomonas vaginalis are treated with the 5-nitroimidazole drug metronidazole, which is also in use against entamoeba histolytica, giardia intestinalis and microaerophilic/anaerobic bacteria. here we report that in t. vaginalis the flavin enzyme thioredoxin reductase displays nitroreductase activity with nitroimidazoles, including metronidazole, and with the nitrofuran drug furazolidone. reactive metabolites of metronidazole and other nitroimidazoles ... | 2009 | 19415801 |
| the nucleolus in entamoeba histolytica and entamoeba invadens is located at the nuclear periphery. | the ribosomal rna genes in the human parasite entamoeba histolytica and its reptilian counterpart entamoeba invadens are located on extrachromosomal circles. the expression of rrna genes generally takes place in a specialized nuclear compartment-the nucleolus. in entamoeba species the nuclear space that may be called the nucleolus has yet to be defined. previous studies showed that the rdna circles are located at the nuclear periphery. here we have raised antibodies against the e. histolytica ho ... | 2009 | 19416742 |
| [cryptosporidium sp infections and other intestinal parasites in food handlers from zulia state, venezuela]. | cryptosporidiosis in food handlers from venezuela is unknown, being this an important public health problem in immunosuppressed patients. to determine the prevalence of cryptosporidium sp and other intestinal parasites in food handlers from zulia state, one hundred nineteen fecal samples were evaluated by wet mount, concentrated according to ritchie and modified ziehl-neelsen staining. fourteen (11.8%) were positive for cryptosporidium sp and associated with other protozoosis (p < 0.05), being m ... | 2009 | 19418723 |
| major cysteine peptidases of entamoeba histolytica are required for aggregation and digestion of erythrocytes but are dispensable for phagocytosis and cytopathogenicity. | cysteine peptidases of entamoeba histolytica (ehcps) are considered to be important pathogenicity factors. it has been described that under standard axenic culture conditions, only three (ehcp-a1, ehcp-a2 and ehcp-a5) out of approximately 50 cysteine peptidase genes present in the e. histolytica genome are substantially expressed, thus representing the set of major ehcps. in this study, transcriptional silencing of the major peptidase genes was used to characterize their physiological role in mo ... | 2009 | 19426210 |
| [autochthonous amebic liver abscess in spain: an emerging disease? case report and description of a pcr-based diagnostic test]. | in spain an increase in cases of amebiasis has been detected in patients with no history of traveling to, or immigration from, endemic areas. | 2009 | 19427082 |
| natural killer t cells activated by a lipopeptidophosphoglycan from entamoeba histolytica are critically important to control amebic liver abscess. | the innate immune response is supposed to play an essential role in the control of amebic liver abscess (ala), a severe form of invasive amoebiasis due to infection with the protozoan parasite entamoeba histolytica. in a mouse model for the disease, we previously demonstrated that jalpha18(-/-) mice, lacking invariant natural killer t (inkt) cells, suffer from more severe abscess development. here we show that the specific activation of inkt cells using alpha-galactosylceramide (alpha-galcer) in ... | 2009 | 19436711 |
| insights into the mechanism of dna recognition by the methylated line binding protein ehmlbp of entamoeba histolytica. | ehmlbp is an essential entamoeba histolytica protein that binds preferentially to methylated long interspersed nuclear elements and rdna. in an effort to identify more ehmlbp dna substrates, we developed an affinity-based technique in which the c-terminal dna binding domain of ehmlbp (gst-cterehmlbp) was used as the ligand. bioinformatic analysis of the dna sequences that were isolated by this affinity method revealed the presence of a 29-nucleotide consensus motif that includes a stretch of ten ... | 2009 | 19450728 |
| amoebic dysentery. | amoebic dysentery is caused by the protozoan parasite entamoeba histolytica. it is transmitted in areas where poor sanitation allows contamination of drinking water and food with faeces. in these areas, up to 40% of people with diarrhoea may have amoebic dysentery. methods and outcomes: we conducted a systematic review and aimed to answer the following clinical question: what are the effects of drug treatments for amoebic dysentery in endemic areas? we searched: medline, embase, the cochrane lib ... | 2007 | 19454043 |
| microscopic diagnosis of sodium acetate-acetic acid-formalin-fixed stool samples for helminths and intestinal protozoa: a comparison among european reference laboratories. | the present study aimed to compare the diagnostic performance of different european reference laboratories in diagnosing helminths and intestinal protozoa, using an ether-concentration method applied to sodium acetate-acetic acid-formalin (saf)-preserved faecal samples. in total, 102 stool specimens were analysed during a cross-sectional parasitological survey in urban farming communities in côte d'ivoire. five saf-preserved faecal samples were prepared from each specimen and forwarded to the pa ... | 2010 | 19456836 |
| diagnosis and treatment of acute or persistent diarrhea. | studies of microbial pathogens and the toxins they produce are important for determining the mechanisms by which they cause disease and spread throughout a population. some bacteria produce secretory enterotoxins (such as cholera toxin or the heat-labile or stable enterotoxins produced by escherichia coli) that invade cells directly. others invade cells or produce cytotoxins (such as those produced by shigella, enteroinvasive e coli, or clostridium difficile) that damage cells or trigger host re ... | 2009 | 19457416 |
| dna characterization of simian entamoeba histolytica-like strains to differentiate them from entamoeba histolytica. | two simian entamoeba histolytica-like strains, ehmfas1 and p19-061405, have been suggested to represent a new species based on genetic characterization. sequence analyses of the hexokinase, glucose phosphate isomerase, and phosphoglucomutase genes supported the previous findings of isoenzyme analyses demonstrating a new zymodeme pattern. phylogenetic studies of 18s rdna, 5.8s rdna, the chaperonin 60 gene, and the pyridine nucleotide transhydrogenase gene showed original clusters of simian e. his ... | 2009 | 19471966 |
| high early mortality in patients with chronic acquired immunodeficiency syndrome diarrhea initiating antiretroviral therapy in haiti: a case-control study. | this case-control study examines whether chronic diarrhea at initiation of antiretroviral therapy (art) affects survival of human immunodefiency virus-infected patients. cases (288) were treatment-naive, non-pregnant, adults with self report of frequent loose stool for > 3 weeks at the time art was initiated. one-third of patients had an enteric pathogen identified including cryptosporidium spp., giardia spp., isospora belli, cyclospora cayetanensis, and entamoeba histolytica. control patients ( ... | 2009 | 19478276 |
| involvement of serine proteases in the excystation and metacystic development of entamoeba invadens. | although the functions of cysteine proteases involved in the pathogenicity and differentiation of entamoeba histolytica have been demonstrated, little is known about the functions of serine proteases. we examined the involvement of serine proteases in amoebic excystation and metacystic development using inhibitors specific for serine proteases. entamoeba invadens ip-1 strain was used as the model of excystation and metacystic development of e. histolytica. four serine protease inhibitors, phenyl ... | 2009 | 19479279 |
| an alternative method for dna extraction and pcr identification of entamoeba histolytica and e. dispar in fecal samples. | since it is known that entamoeba dispar is non-pathogenic and morphologically similar to e. histolytica, there are many targets used in pcr for differentiating these species. however, obtaining high quality dna from fecal samples is fundamental for pcr. most methods are laborious or use kits that make diagnosis expensive. in the present work, a new simple, fast and cheap technique of dna extraction from fecal samples was combined with a pcr for an episomal target in order to identify e. histolyt ... | 2009 | 19486545 |
| [the complicated intestinal amebiasis in emergency surgery]. | 18 patients with complicated forms of intestinal amebiasis were operated on acute appendicitis, liver abscess or total necrotic colitis. appendectomy, abscess drainage and colon resection were performed respectively. there were no postoperative deaths. features of amebic appendicitis and total necrotic amebic colitis are described using clinical cases demonstrations. recommendations for the treatment of these forms of amebiasis are given. | 2009 | 19491760 |
| phosphatidylinositol-phosphates mediate cytoskeletal reorganization during phagocytosis via a unique modular protein consisting of rhogef/dh and fyve domains in the parasitic protozoon entamoeba histolytica. | to understand the roles of phosphoinositides [ptdins] in phagocytosis of parasitic eukaryotes, we examined the interaction of phosphatidylinositol-3-phosphate [ptdins(3)p] and putative ptdins-p-binding proteins during phagocytosis in the enteric protozoan parasite entamoeba histolytica. it was previously shown that phagocytosis in e. histolytica is indispensable for virulence and is inhibited by ptdins 3-kinase inhibitors. we demonstrated by time-lapse live imaging that during the initiation of ... | 2009 | 19496789 |
| periodicity and patterns of entamoeba histolytica and e. dispar infection in hiv+/aids patients in mexico. | in a 12-month longitudinal study, a cohort of mexican hiv+/aids patients was checked several times for entamoeba infection, with the parasites identified, as e. histolytica or e. dispar, using pcr. the polymorphic region of the parasites' chitinase genes was investigated by pcr, with the variation in amplicon sizes being used as a measure of the genetic variation among the isolates. the patients found infected with entamoeba at the start of the study displayed varied patterns of infection cleara ... | 2009 | 19508748 |
| entamoeba histolytica, heterologous expression and in situ immunolocalization of ornithine decarboxylase (ehodc). | previous studies from this laboratory have dealt with the purification and biochemical characterization of ornithine decarboxylase (odc) from entamoeba histolytica. enzyme compartmentalization has been described as a major mechanism in the regulation of polyamine metabolism. however, the subcellular location of odc in the human parasite has remained unresolved. to examine this issue, we cloned the full-length gene (ehodc) encoding for the parasite enzyme, whose open reading frame encodes for a p ... | 2009 | 19520076 |
| high genetic diversity among iranian entamoeba dispar isolates based on the noncoding short tandem repeat locus d-a. | this study has identified and characterized the structure of locus d-a, a noncoding short tandem repeat (str) region, also known as locus 1-2, in iranian entamoeba dispar isolates. this polymorphic locus has been shown to be potentially useful in investigating the molecular epidemiology of entamoeba histolytica and e. dispar. the genetic polymorphisms in locus d-a in 28 isolates of e. dispar from three different geographic regions of iran were distinguished using pcr and sequencing, and the resu ... | 2009 | 19524081 |
| concurrent infections and socioeconomic determinants of geohelminth infection: a community study of schoolchildren in periurban guinea-bissau. | we explored the association between subclinical intestinal helminth infections and other gastrointestinal pathogens in 706 schoolchildren from a poor semirural area while adjusting for socioeconomic risk factors. the study was carried out in two neighbouring areas in the capital of guinea-bissau in west africa. children aged 4-12 years were visited and one child per mother was invited to participate in the study. among the 706 children included in the study, helminths were detected in 44.2%, ent ... | 2009 | 19524992 |
| use and endocytosis of iron-containing proteins by entamoeba histolytica trophozoites. | iron is essential for nearly all organisms; in mammals, it is part of proteins such as haemoglobin, and it is captured by transferrin and lactoferrin. transferrin is present in serum, and lactoferrin is secreted by the mucosa and by neutrophils at infection sites, as a host iron-withholding response, sequestering iron away from invading microorganisms. additionally, all cells contain ferritin, which sequesters iron when its intracellular levels are increased, detoxifying and preventing damage. l ... | 2009 | 19539057 |
| reassessment of the epidemiology of amebiasis: state of the art. | the epidemiology of amebiasis has dramatically changed since the separation of entamoeba histolytica and entamoeba dispar species, and the worldwide prevalence of these species has not been estimated until recently. the most cited data regarding prevalence, morbidity, or mortality due to amebiasis is the 1986 walsh report, in which 100,000 deaths are reported to occur worldwide each year due to medical complications of invasive amebiasis. however, the prevalence values of entamoeba histolytica i ... | 2009 | 19540361 |
| [remarkable evolution of hepatic, pleural and pulmonary amoebiasis: a case report]. | entamoeba histolytica, is a human pathogen which is endemic in tropical areas. the most common extra-intestinal locations for disease after the liver are pleural and pulmonary. although the outcome of pleural and pulmonary amoebiasis is generally favourable, it is important to note that a significant proportion of patients do develop pleural and pulmonary after-effects. we report a case of pleural and pulmonary amoebiasis where the evolution of a massive pleural effusion and 3 associated liver a ... | 2009 | 19543174 |
| [tinidazole: a classical anaerobical drug with multiple potential uses nowadays]. | tinidazole is a 5-nitroimidazole active in vitro against a wide variety of anaerobic bacteria and protozoa. tinidazole is an effective treatment against anaerobic microorganisms based on its pharmacokinetic characteristics (c(max) 51 microg/ml, t(1/2) 12.5 h) and its excellent in vitro activity. its long half-life allows once a day regimens. tinidazole is as effective as metronidazole in the treatment of infections caused by t. vaginalis, giardiasis and amebiasis and bacterial vaginosis, malaria ... | 2009 | 19544102 |
| hospital-based surveillance of enteric parasites in kolkata. | diarrhoea is the second leading cause of illness and death in developing countries and the second commonest cause of death due to infectious diseases among children under five in such countries. parasites, as well as bacterial and viral pathogens, are important causes of diarrhoea. however, parasitic infections are sometimes overlooked, leading after a period of time to an uncertain aetiology. in this paper we report the prevalence of giardia lamblia, entamoeba histolytica and cryptosporidium sp ... | 2009 | 19545355 |
| protection against intestinal amebiasis by a recombinant vaccine is transferable by t cells and mediated by gamma interferon. | we have previously shown that vaccination with purified entamoeba histolytica gal/galnac lectin or recombinant subunits can protect mice from intestinal amebiasis upon intracecal challenge. in this study, we demonstrated with adoptive-transfer experiments that this lectin vaccine protection is mediated by t cells but not serum. the cell-mediated immune (cmi) response was characterized by significant gamma interferon (ifn-gamma), interleukin 12 (il-12), il-2, il-10, and il-17 production. to move ... | 2009 | 19564375 |
| cell-free synthesis and combinatorial selective 15n-labeling of the cytotoxic protein amoebapore a from entamoeba histolytica. | amoebapore a is a pore-forming protein produced by the pathogenic parasite entamoeba histolytica, which causes human amoebic dysentery. the pore-forming activity of amoebapore a is regulated by ph-dependent dimerization, a prerequisite for membrane insertion and pore formation. understanding of these important processes has been hampered by the cytotoxicity of amoebapore a, which prevents the production of this protein in cell-based expression systems. in this study, a protocol for the cell-free ... | 2009 | 19573603 |
| intestinal amoebiasis in children and its effect on nutritional status. | to determine the frequency and clinical presentation of amoebic diarrhea in children and its effect on the nutritional status of the affected children. | 2009 | 19576153 |
| physical, structural, and functional properties of the beta1 integrin-like fibronectin receptor (beta1ehfnr) in entamoeba histolytica. | the presence in entamoeba histolytica of a fibronectin (fn) receptor, which is antigenically related to beta1 integrin-like molecules and shows 99% homology with the intermediate subunit-2 of the gal/galnac-specific lectin has been described. the e. histolytica genome has been sequenced, and its analysis shows no integrin sequences. here we provide further evidence to demonstrate that this molecule behaves as integrin-like in its physical, structural and functional properties. the purified beta1 ... | 2009 | 19577014 |
| evidence for a "wattle and daub" model of the cyst wall of entamoeba. | the cyst wall of entamoeba invadens (ei), a model for the human pathogen entamoeba histolytica, is composed of fibrils of chitin and three chitin-binding lectins called jacob, jessie3, and chitinase. here we show chitin, which was detected with wheat germ agglutinin, is made in secretory vesicles prior to its deposition on the surface of encysting ei. jacob lectins, which have tandemly arrayed chitin-binding domains (cbds), and chitinase, which has an n-terminal cbd, were each made early during ... | 2009 | 19578434 |
| a possible cluster of sexually transmitted entamoeba histolytica: genetic analysis of a highly virulent strain. | transmission of entamoeba histolytica generally occurs by fecal excretion of cysts followed by oral ingestion of contaminated food or water. however, fecal-oral transmission may occur within households and long-term care institutions, and sexual transmission occurs among men who have sex with men. epidemiologically linked clusters of e. histolytica infection are rare in industrialized countries. we report such a sexually linked cluster in canada. | 2009 | 19580413 |
| crude extracts of, and purified compounds from, pterocarpus angolensis, and the essential oil of lippia javanica: their in-vitro cytotoxicities and activities against selected bacteria and entamoeba histolytica. | in a recent study, various extracts of pterocarpus angolensis were prepared and tested against bacteria. the acetone extract was found to be the most active against all the bacteria investigated, with minimum inhibitory concentrations varying from 0.0156 mg/ml against staphylococcus aureus to 2 mg/ml against enterobacter cloacae. seven pure compounds were subsequently isolated from the ethanol extract of p. angolensis. using several chromatographic and spectroscopic methods, the structures of fi ... | 2009 | 19583913 |
| aids-associated parasitic diarrhoea. | since the advent of human immunodeficiency virus infection, with its profound and progressive effect on the cellular immune system, a group of human opportunistic pathogens has come into prominence. opportunistic parasitic infection can cause severe morbidity and mortality. because many of these infections are treatable, an early and accurate diagnosis is important. this can be accomplished by a variety of methods such as direct demonstration of parasites and by serological tests to detect antig ... | 2009 | 19584497 |
| rapid diagnosis of intestinal parasitic protozoa, with a focus on entamoeba histolytica. | entamoeba histolytica is an invasive intestinal pathogenic parasitic protozoan that causes amebiasis. it must be distinguished from entamoeba dispar and e. moshkovskii, nonpathogenic commensal parasites of the human gut lumen that are morphologically identical to e. histolytica. detection of specific e. histolytica antigens in stools is a fast, sensitive technique that should be considered as the method of choice. stool real-time pcr is a highly sensitive and specific technique but its high cost ... | 2009 | 19584941 |
| synthesis, characterization, antiamoebic activity and toxicity of novel bisdioxazole derivatives. | cyclization of benzene-1,4-dicarbaldehyde dioxime 1 with different aromatic aldehydes in inert atmosphere yielded the corresponding new bisdioxazoles 2-11. the structure of 2-11 was elucidated by spectral data. in vitro antiamoebic activity was performed against hm1:imss strain of entamoeba histolytica. the results showed that the compounds 3 (ic(50)=1.22 microm), 4 (ic(50)=1.41 microm), 7 (ic(50)=1.05 microm) and 10 (ic(50)=1.01 microm) exhibited better antiamoebic activity than the standard dr ... | 2009 | 19589625 |
| dna repair mechanisms in eukaryotes: special focus in entamoeba histolytica and related protozoan parasites. | eukaryotic cell viability highly relies on genome stability and dna integrity maintenance. the cellular response to dna damage mainly consists of six biological conserved pathways known as homologous recombination repair (hrr), non-homologous end-joining (nhej), base excision repair (ber), mismatch repair (mmr), nucleotide excision repair (ner), and methyltransferase repair that operate in a concerted way to minimize genetic information loss due to a dna lesion. particularly, protozoan parasites ... | 2009 | 19591963 |
| [antigen detection methods in diagnosis of amebiasis, giardiasis and cryptosporidiosis]. | amebiasis is a significant health problem in developing countries. humans are infected by two morphologically identical species of entamoeba. entamoeba histolytica causes amebic colitis and liver abscess, and entamoeba dispar is noninvasive. giardia intestinalis infection is seen worldwide and in all age groups. but giardiasis is especially prevalent in countries with poor sanitation and unsafe water, where it's responsible for most cases of childhood diarrhea. cryptosporidium parvum, a protozoo ... | 2009 | 19598091 |
| evaluation of the antiprotozoal activity of neo-clerodane type diterpenes from salvia polystachya against entamoeba histolytica and giardia lamblia. | chia (salvia polystachya ort., lamiaceae) is frequently used in mexican traditional medicine to treat dysentery. in this study the main neo-clerodane diterpenes (polystachynes a, b and d, as well as linearolactone) were isolated from the aerial parts of chia, and their antiprotozoal activities toward entamoeba histolytica and giardia lamblia trophozoites were evaluated in vitro. linearolactone was the most potent antiamoebic and antigiardial compound with ic(50) values of 22.9 microm for e. hist ... | 2010 | 19610033 |
| a rare but fatal case of granulomatous amebic encephalitis with brain abscess: the first case reported from turkey. | the incidence of protozoal and helminthic infestations of the central nervous system (cns) is less than 1%, but these infestations tend to follow a fatal course. they are more common among children, the elderly and immunocompromised individuals. cns infections due to entamoeba histolytica have been known for a long time. in recent years, especially in developing countries, there has been an increase in cns infections due to free-living amebas (flas). acute cns infection due to naegleria fowleri, ... | 2009 | 19621290 |
| prevalence of intestinal parasitosis in a rural population in egypt, and its relation to socio-demographic characteristics. | a cross-sectional study was conducted in a village in menoufia governorate, egypt where the majority of people had individual trenches in the houses for sewage disposal with absence of public sewage system. out of 2292 stool samples 47.8% had at least a single infection. multiple infections occurred in 14.9%. entamoeba histolytica was 20%, e. coli 10%, giardia lamblia 10%, ascaris lumbricoides 27.31%, hymenolepis nana 2.96%, schistosoma mansoni 2.45% and ancylostoma duodenale 2.23%. males were s ... | 2009 | 19621655 |
| [sensibility of entamoeba histolytica trophozoites to ivermectin]. | amebiasis caused by entamoeba histolytica is a problem of public world health. the most frequent clinical presentation are the dysentery and the amebic liver abscess. fifty millions of cases and more than 100.000 deaths for this disease are reported annually worldwide. the life cycle of e. histolytica has two phases: trophozoite and cyst. trophozoites are the causal agent of disease. the effective treatment for the amebiasis includes drugs with serious collateral effects. ivermectin is a macroli ... | 2009 | 19622479 |
| parasitological diagnosis combining an internally controlled real-time pcr assay for the detection of four protozoa in stool samples with a testing algorithm for microscopy. | molecular detection of gastrointestinal protozoa is more sensitive and more specific than microscopy but, to date, has not routinely replaced time-consuming microscopic analysis. two internally controlled real-time pcr assays for the combined detection of entamoeba histolytica, giardia lamblia, cryptosporidium spp. and dientamoeba fragilis in single faecal samples were compared with triple faeces test (tft) microscopy results from 397 patient samples. additionally, an algorithm for complete para ... | 2009 | 19624500 |
| entamoeba dispar: genetic diversity of iranian isolates based on serine-rich entamoeba dispar protein gene. | the nucleotide sequences of serine-rich entamoeba histolytica protein (srehp) gene have already exhibited stable and significant polymorphism in the gene studies. serine-rich protein is also present and polymorphic in entamoeba dispar which called sredp. the polymorphism of the serine-rich entamoeba dispar protein (sredp) gene among 8 isolates obtained from iranian cyst carriers were analyzed by a nested pcr-rflp followed by sequencing of the pcr products. from those isolates, six distinct dna p ... | 2008 | 19630212 |
| sequence specific 1h, 13c and 15n resonance assignments of a calmodulin-like calcium-binding protein from the protozoan parasite entamoeba histolytica (ehcam). | we report almost complete sequence specific 1h, 13c and 15n nmr assignments of a 151-residue long calmodulin-like calcium-binding protein from entamoeba histolytica (ehcam). | 2008 | 19636930 |
| gonadectomy inhibits development of experimental amoebic liver abscess in hamsters through downregulation of the inflammatory immune response. | incidence of amoebic liver abscess (ala) in human males is considerably higher than in females, suggesting a role for sex hormones in this parasite infection. we describe here the effect of hamster gonadectomization on the development of ala. after monitoring the decrease of oestradiol in females and testosterone in males to undetectable levels by elisa and radio immuno assay (ria) in serum, hamsters were intraportally infected with entamoeba histolytica trophozoites and killed 7 days later. ala ... | 2009 | 19646209 |
| entamoeba invadens: cloning and molecular characterization of chitinases. | entamoeba histolytica, the causative agent of amebiasis infects through its cyst form and this transmission may be blocked using encystation specific protein as drug target. in this study, we have characterized the enzyme chitinase which express specifically during encystation. the reptilian parasite entamoeba invadens, used as a model for encystation study contain three chitinases. we report the molecular cloning, over-expression and biochemical characterization of all three e. invadens chitina ... | 2009 | 19646441 |
| comparison of two genetically related entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties. | entamoeba histolytica is known for its extraordinary capacity to destroy human tissues, leading to invasive diseases such as ulcerative colitis or extra-intestinal abscesses. in order to identify the virulence factors of this parasite phenotypes and proteomes of two recently identified genetically related cell lines (a and b), derived from the laboratory e. histolytica isolate hm-1:imss, were compared. both cell lines are indistinguishable on the basis of highly polymorphic tandem repeat dna seq ... | 2009 | 19688750 |
| in vitro activity of pyrvinium pamoate against entamoeba histolytica and giardia intestinalis using radiolabelled thymidine incorporation and an sybr green i-based fluorescence assay. | to assess the in vitro activity of the fda-approved antihelminthic drug pyrvinium pamoate against entamoeba histolytica and giardia intestinalis. | 2009 | 19689977 |
| opportunistic parasitic infections in hiv/aids patients presenting with diarrhoea by the level of immunesuppression. | enteric parasites are major cause of diarrhoea in hiv infected individuals. the present study was undertaken to detect enteric parasites in hiv infected patients with diarrhoea at different levels of immunity. | 2009 | 19700803 |
| new synthesis and antiparasitic activity of model 5-aryl-1-methyl-4-nitroimidazoles. | a number of 5-aryl-1-methyl-4-nitroimidazoles 5a-f have been synthesized in good yields by the suzuki coupling reaction between 5-chloro-1-methyl-4-nitroimidazole (3) and arylboronic acids 4a-f, aided by dichlorobis-(triphenylphosphine)palladium(ii), k(2)co(3, )and tetrabutylammonium bromide in water at 70-80 degrees c. compounds 5a-f were characterized by elemental analysis, nmr and ms spectral data. on the basis of in vitro screening data, 5-(3-chlorophenyl)-1-methyl-4-nitro-1h-imidazole (5f) ... | 2009 | 19701122 |
| endoplasmic reticulum continuity in the protozoan parasite entamoeba histolytica: evolutionary implications and a cautionary note. | entamoeba histolytica has been described as an early branching eukaryotic parasite based on the lack of organelles such as mitochondria and peroxisomes, and on morphologic studies that concluded it possesses a vesicular endoplasmic reticulum (er) and golgi complex. however, a recent study from our laboratory showed that the e. histolytica er is continuous by using an er-targeted green fluorescent protein fusion protein and photobleaching experiments. we proposed that the vesicular er seen earlie ... | 2008 | 19704884 |
| presence and diagnosis of amebic infestation in turkish patients with active ulcerative colitis. | it is difficult to definitively diagnose acute amebiasis, particularly when this condition is superimposed on inflammatory bowel disease. our goals in this study were to determine the prevalence of amebiasis in individuals with active ulcerative colitis, and to identify clinical and laboratory parameters that are of value for diagnosing amebiasis in this patient group. | 2009 | 19712863 |
| intestinal parasitic infections among inhabitants of karaj city, tehran province, iran in 2006-2008. | karaj is an area with large influx of refugee people in iran. to increase knowledge about parasitic infections, we carried out this research during 2006-2008. we recorded the stool examination results and some of their personal characteristics. a total of 13,915 human stools were examined, and 649 (4.7%) were positive for intestinal parasites. among them, 13 (0.09%) had worm and 636 (4.6%) had protozoan infections. maximum infections belonged to giardia intestinalis, and 534 (3.8%) samples had t ... | 2009 | 19724700 |
| a survey of intestinal protozoan infections among gastroenteritis patients during a 3-year period (2004-2006) in gyeonggi-do (province), south korea. | the incidence and etiology of parasite-associated gastroenteritis during 2004-2006 in gyeonggi-do (province), south korea was determined by means of antigen detection elisa on 6,071 stool specimens collected from 6 general hospitals. at least 1 parasitic agent was detected in 3.4% (208/6,071) of the stool samples. among these, giardia lamblia was the most numerous (152 cases; 2.5%), followed by entamoeba histolytica (25 cases; 0.4%), cryptosporidium parvum (23 cases; 0.4%), and mixed infections ... | 2009 | 19724707 |
| malnutrition and intestinal parasitic infections in school children of gondar, north west ethiopia. | in developing countries, malnutrition is a considerable health problem with prevalence ranges of 4-46%, with 1-10% severely malnourished. | 2009 | 19743775 |
| a case report of pulmonary amoebiasis with entamoeba histolytica diagnosed in western romania. | among parasitic diseases, amoebiasis is the third most frequent cause of mortality after malaria and schistosomiasis. pulmonary amoebiasis, the second most common extraintestinal manifestation of infection, occurs in 2%-3% of patients with invasive amoebiasis. the present clinical report aims to present a single case of a rare diagnosed parasitic disease in romania and to emphasize the difficulties encounted while attempting to establish the correct diagnosis and to completely cure the patient. | 2008 | 19745512 |
| prevalence of multi-gastrointestinal infections with helminth, protozoan and campylobacter spp. in guatemalan children. | the prevalence of multi-infections with helminthes, protozoans and campylobacter spp. in guatemalan children is a reflection of differences in the risk factors related to pathogen transmission. | 2009 | 19759480 |
| [prevalence and polyparasitism of intestinal protozoa and spatial distribution of entamoeba histolytica, e. dispar and giardia intestinalis from pupils in the rural zone of man in côte d'ivoire]. | diseases caused by environmental contamination by micro-organisms, including intestinal helminths and protozoa, are prevalent in developing countries. according to some authors, their strong expansion in some zones of these countries is due primarily to favourable climatic conditions, combined with inadequate hygiene measures and cleaning and the generally low socio-economic level. progress in disease control has resulted from new studies that improve our understanding of the epidemiology of hel ... | 2008 | 19810617 |
| genomic dna k-mer spectra: models and modalities. | the empirical frequencies of dna k-mers in whole genome sequences provide an interesting perspective on genomic complexity, and the availability of large segments of genomic sequence from many organisms means that analysis of k-mers with non-trivial lengths is now possible. | 2009 | 19814784 |
| intestinal parasitic infection and associated risk factors, among children presenting at outpatient clinics in manaus, amazonas state, brazil. | in a cross-sectional study, the prevalences of intestinal parasitic infection and the associated risk factors were explored among children aged 0-10 years attending outpatient clinics in manaus, the capital city of the north brazilian state of amazonas. data indicating socio-economic level and demographic factors were collected in interviews with the accompanying parent or guardian of each child. parasitic infections were detected by the microscopical examination of faecal samples, while nutriti ... | 2009 | 19825280 |
| deficient serum mannose-binding lectin levels and mbl2 polymorphisms increase the risk of single and recurrent cryptosporidium infections in young children. | mannose-binding lectin (mbl) is an evolutionarily conserved protein that functions in human innate immunity by binding to microbial surfaces and promoting opsonophagocytosis. mbl has been shown to bind to cryptosporidium sporozoites, and earlier work has suggested that the protective role of mbl may be most important in childhood. we evaluated the association between polymorphisms in the mbl gene (mbl2), serum mbl deficiency, and infection with cryptosporidium, entamoeba histolytica, and giardia ... | 2009 | 19827946 |
| identification of entamoeba histolytica and entamoeba dispar by pcr assay of fecal specimens obtained from thai/myanmar border region. | due to the indistinguishable morphology between entamoeba histolytica (pathogenic) and entamoeba dispar (non pathogenic), pcr-based assays were conducted. based on microscopy, suspected entamoeba cells were detected in 30 out of 455 fecal samples obtained from individuals residing at thai/myanmar border region. the target genes for pcr amplification included genes encoding small subunit rrna (ssu-rrna), chitinase and serine rich entamoeba protein. pcr primers derived from ssu-rrna gene amplified ... | 2009 | 19842426 |
| pathema: a clade-specific bioinformatics resource center for pathogen research. | pathema (http://pathema.jcvi.org) is one of the eight bioinformatics resource centers (brcs) funded by the national institute of allergy and infectious disease (niaid) designed to serve as a core resource for the bio-defense and infectious disease research community. pathema strives to support basic research and accelerate scientific progress for understanding, detecting, diagnosing and treating an established set of six target niaid category a-c pathogens: category a priority pathogens; bacillu ... | 2010 | 19843611 |
| epigenetic transcriptional gene silencing in entamoeba histolytica: insight into histone and chromatin modifications. | we have previously discovered a unique mechanism of epigenetic transcriptional gene silencing in the entamoeba histolytica trophozoites of strain hm-1:imss that resulted in the persistent downregulation of the amoebapore a (ap-a) gene, and that could be successfully applied to silence other virulence genes (cpa5, lgl1). in order to understand how the silencing is maintained throughout generations, we analysed whether modifications occurred at the chromatin level. chromatin immunoprecipitation as ... | 2010 | 19849886 |
| serum leptin concentrations in patients with intestinal parasites. | leptin is a protein hormone controlling food intake and energy expenditure. in all infections including parasitic infections there is loss of appetite and anorexia. the aim of the present study was to clarify the relationship between intestinal parasites and serum leptin concentrations in children and adults. forty patients with intestinal parasites and 34 healthy subjects took part in this study. body weight, height and body mass index (bmi) were measured for all patients and controls. patients ... | 2009 | 19851965 |
| [epidemiological investigation on an outbreak of amoebic dysentery in jiangshan city of zhejiang province]. | the investigation was carried out from 15 may to 10 june 2006 among diarrhea patients of two schools and periphery residents in hecun town, jiangshan city. stool samples were examined for entamoeba histolytica. water samples were taken for microbial analysis. 31 cases with e. histolytica were found,with 74.2% (23 cases) of students and preschool children. 9 cases were found in liuyi kindergarten and 8 cases in hecun central primary school with a prevalence of 7.4% and 0.65%, respectively. among ... | 2009 | 19856515 |
| methionine gamma-lyase: the unique reaction mechanism, physiological roles, and therapeutic applications against infectious diseases and cancers. | sulfur-containing amino acids (saas) are essential components in many biological processes and ubiquitously distributed to all organisms. both biosynthetic and catabolic pathways of saas are heterogeneous among organisms and between developmental stages, and regulated by the environmental changes. limited lineage of organisms ranging from archaea to plants, but not human, possess a unique enzyme methionine gamma-lyase (mgl, ec 4.4.1.11) to directly degrade saa to alpha-keto acids, ammonia, and v ... | 2009 | 19859976 |
| entamoeba histolytica and e. dispar infections in captive macaques (macaca fascicularis) in the philippines. | entamoeba histolytica is a protozoan parasite that infects man and animals. this parasite has a global distribution and the disease it causes is usually characterized by diarrhea. in order to detect the parasite, it is necessary to differentiate it from entamoeba dispar. e. dispar appears morphologically similar to e. histolytica but does not cause disease and tissue invasion. this study reports on the prevalence of e. histolytica and e. dispar among captive macaques in a primate facility in the ... | 2010 | 19862480 |
| the encystment of dysentery amebae in vitro. | dysentery amebae may live for 72 hours in vitro. the transition of the large vegetative forms into the cyst may be observed in vitro. the large vegetative ameba always passes into an intermediate stage before assuming the small vegetative form. the atypical forms are dysentery amebas with abnormal nuclei and cytoplasm. when circumstances are unfavorable encystment takes place. under favorable conditions, on the other hand, they may again change into typical forms. the majority of the "degenerati ... | 1918 | 19868266 |
| crystal structure of the aspartyl-trna synthetase from entamoeba histolytica. | the crystal structure of the aspartyl-trna synthetase from the eukaryotic parasite entamoeba histolytica has been determined at 2.8aresolution. relative to homologous sequences, the e. histolytica protein contains a 43-residue insertion between the n-terminal anticodon binding domain and the c-terminal catalytic domain. the present structure reveals that this insertion extends an arm of the hinge region that has previously been shown to mediate interaction of aspartyl-trna synthetase with the co ... | 2010 | 19874856 |
| characterization of the restriction enzyme-like endonuclease encoded by the entamoeba histolytica non-long terminal repeat retrotransposon ehline1. | the genome of the human pathogen entamoeba histolytica, a primitive protist, contains non-long terminal repeat retrotransposable elements called ehlines. these encode reverse transcriptase and endonuclease required for retrotransposition. the endonuclease shows sequence similarity with bacterial restriction endonucleases. here we report the salient enzymatic features of one such endonuclease. the kinetics of an ehline1-encoded endonuclease catalyzed reaction, determined under steady-state and si ... | 2009 | 19878305 |
| [pleuro-pulmonary manifestations disclosing hepatic amoebiasis]. | during a mission in ex-yugoslavia between 2001 and 2004, three french soldiers were sent home because of right pneumopathy, right pleurisy after appendicectomy, haemoptysis and liver haematoma, respectively. they previously were stationed in africa and/or south america. the initial diagnosis was quickly modified: pleuropulmonary manifestations of amoebic hepatic abscess in two cases, and pleuropulmonary amoebiasis in the last case. the outcome was favourable with standard anti-amoebic treatment. ... | 2009 | 19878805 |
| bacterial-type oxygen detoxification and iron-sulfur cluster assembly in amoebal relict mitochondria. | the assembly of vital reactive iron-sulfur (fe-s) cofactors in eukaryotes is mediated by proteins inherited from the original mitochondrial endosymbiont. uniquely among eukaryotes, however, entamoeba and mastigamoeba lack such mitochondrial-type fe-s cluster assembly proteins and possess instead an analogous bacterial-type system acquired by lateral gene transfer. here we demonstrate, using immunomicroscopy and biochemical methods, that beyond their predicted cytosolic distribution the bacterial ... | 2010 | 19888992 |