| non-immune-related genes and signalling pathways in spleen of vibrio parahaemolyticus-infected epinephelus fuscoguttatus (forskal). | | 2015 | 25073481 |
| prevalence of vibrio parahaemolyticus, and vibrio vulnificus in blue crabs (callinectes sapidus), seawater and sediments of the maryland coastal bays. | to determine the prevalence of total and pathogenic vibrio parahaemolyticus (vp) and v. vulnificus (vv) in blue crabs, water and sediment from the maryland coastal bays (mcbs), usa. | 2014 | 25066367 |
| simultaneous irradiation with different wavelengths of ultraviolet light has synergistic bactericidal effect on vibrio parahaemolyticus. | ultraviolet (uv) irradiation is an increasingly used method of water disinfection. uv rays can be classified by wavelength into uva (320-400 nm), uvb (280-320 nm), and uvc (<280 nm). we previously developed uva sterilization equipment with a uva light-emitting diode (led). the aim of this study was to establish a new water disinfection procedure using the combined irradiation of the uva-led and another uv wavelength. an oxidative dna product, 8-hydroxy-2'-deoxyguanosine (8-ohdg), increased after ... | 2014 | 25041035 |
| the possible use of v. parahaemolyticus - specific bacteriophages for prevention and therapy of infections caused by v. parahaemolyticus. | vibrio parahaemolyticus is the most common halophilic vibrio species causing serious gastroenteritis in humans. the main source of infection is consumption of undercooked or raw seafood or exposure to contaminated water. the monitoring conducted in 2006-2008 demonstrated that warm, subtropical climate and low- to moderate salinity of water in the black sea coastal zone provides a favorable environment for growth and spread of v. parahaemolyticus bacteria. antibiotics are commonly applied for con ... | 2014 | 25020179 |
| toll-like receptor of mud crab, scylla serrata: molecular characterisation, ontogeny and functional expression analysis following ligand exposure, and bacterial and viral infections. | toll-like receptors are sentinels of innate immune system, which recognise pathogen-associated molecular patterns, and subsequently activate production of antimicrobial peptides to contain the infection. in the present study, we cloned and characterised a toll gene from scylla serrata (sstoll) encoding 1005 amino acids with typical toll-like receptor domain topology. phylogenetic analysis revealed that it belongs to insect-type invertebrate toll family showing 100 % identity with scylla paramamo ... | 2014 | 25012916 |
| the effect of storage time on vibrio spp. and fecal indicator bacteria in an isco autosampler. | monitoring concentrations of bacterial pathogens and indicators of fecal contamination in coastal and estuarine ecosystems is critical to reduce adverse effects to public health. during storm events, particularly hurricanes, floods, nor'easters, and tropical cyclones, sampling of coastal and estuarine waters is not generally possible due to safety concerns. it is particularly important to monitor waters during these periods as it is at precisely these times that pathogenic bacteria such as vibri ... | 2014 | 25008356 |
| [difference of three standard curves of real-time reverse-transcriptase pcr in viable vibrio parahaemolyticus quantification]. | we evaluated the difference of three standard curves in quantifying viable vibrio parahaemolyticus in samples by real-time reverse-transcriptase pcr (real-time rt-pcr). | 2014 | 25007650 |
| risk factors for sporadic vibrio parahaemolyticus gastroenteritis in east china: a matched case-control study. | summary to determine risk factors for sporadic vibrio parahaemolyticus gastroenteritis, we conducted a population-based case-control study in sentinel hospital surveillance areas of shanghai and jiangsu province, china. seventy-one patients with diarrhoea and confirmed v. parahaemolyticus infections were enrolled, and they were matched with 142 controls for gender, age and residential area. from the multivariable analysis, v. parahaemolyticus infections were associated with antibiotics taken dur ... | 2015 | 24992005 |
| most-probable-number loop-mediated isothermal amplification-based procedure enhanced with k antigen-specific immunomagnetic separation for quantifying tdh(+) vibrio parahaemolyticus in molluscan shellfish. | although thermostable direct hemolysin-producing (tdh(+)) vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis, the enumeration of tdh(+) v. parahaemolyticus remains challenging due to its low densities in the environment. in this study, we developed a most-probable-number (mpn)-based procedure designated a-is(1)-lamp, in which an immunomagnetic separation (ims) technique targeting as many as 69 established k antigens and a loop-mediated isothermal amplification (lamp) a ... | 2014 | 24988012 |
| seasonal abundance of total and pathogenic vibrio parahaemolyticus isolated from american oysters harvested in the mandinga lagoon system, veracruz, mexico: implications for food safety. | the abundance of total and pathogenic vibrio parahaemolyticus (vp) strains in american oysters (crassostrea virginica) harvested in two different harvest sites from the mandinga lagoon system was evaluated monthly for 1 year (january through december 2012). frequencies of species-specific genes and pathogenic genes exhibited a seasonal distribution. the annual occurrence of vp with the species-specific tlh gene (tlh(+)) was significantly higher during the winter windy season (32.50%) and spring ... | 2014 | 24988011 |
| h-ns regulates the vibrio parahaemolyticus type vi secretion system 1. | the marine bacterium vibrio parahaemolyticus, a major cause of food-borne gastroenteritis, employs a type vi secretion system 1 (t6ss1), a recently discovered protein secretion system, to combat competing bacteria. environmental signals such as temperature, salinity, cell density and surface sensing, as well as the quorum-sensing master regulator opar, were previously reported to regulate t6ss1 activity and expression. in this work, we set out to identify additional transcription regulators that ... | 2014 | 24987102 |
| development of a colony hybridization method for the enumeration of total and potentially enteropathogenic vibrio parahaemolyticus in shellfish. | vibrio parahaemolyticus is a marine microorganism, recognized as cause of gastroenteritis outbreaks associated with seafood consumption. in this study the development and the in-house validation of a colony hybridization method for the enumeration of total and potentially pathogenic v. parahaemolyticus is reported. the method included a set of three controls (process, hybridization and detection control) for the full monitoring of the analytical procedure. four digoxigenin-labeled probes were de ... | 2014 | 24984219 |
| occurrence of vibrio parahaemolyticus and vibrio vulnificus in retail raw oysters from the eastern coast of thailand. | occurrence, population density and virulence of vibrio parahaemolyticus and v. vulnificus in 240 retail raw oysters collected monthly between march 2010 and february 2011 from ang sila coast, chon buri province, thailand were determined using most probable number (mpn) multiplex pcr. multiplex pcr detected v. parahaemolyticus in 219 raw oyster samples, of which 29 samples contained the virulence tdh. mpn values for v. parahaemolyticus and pathogenic strains in most samples ranged from 10 to 10(2 ... | 2014 | 24974651 |
| draft genome sequence of trh+ vibrio parahaemolyticus vp-49, isolated from seafood harvested along the mangalore coast, india. | vibrio parahaemolyticus is a seafood-borne pathogen autochthonous to the marine and estuarine ecosystem, which is responsible for gastroenteritis due to the consumption of contaminated raw seafood. here, we report the draft genome sequence of v. parahaemolyticus vp-49, isolated from seafood, to identify the different virulence attributes and to study the mechanisms that enhance its environmental fitness. | 2014 | 24970827 |
| dietary effect of sargassum wightii fucoidan to enhance growth, prophenoloxidase gene expression of penaeus monodon and immune resistance to vibrio parahaemolyticus. | the polysaccharide fucoidan from brown seaweed sargassum wightii was extracted and it was incorporated with pellet diets at three concentrations (0.1, 0.2 & 0.3%). the fucoidan incorporated diets were fed to shrimp penaeus monodon for 60 days and the growth performance was assessed. the weight gain and sgr of control group was 6.83 g and 9.72%, respectively, but the weight gain and sgr of various concentrations (0.1-0.3%) of fucoidan incorporated diets fed groups of shrimp was increased from 7.3 ... | 2014 | 24925762 |
| multiplex real-time pcr assay for detection of pathogenic vibrio parahaemolyticus strains. | foodborne disease caused by pathogenic vibrio parahaemolyticus has become a serious public health problem in many countries. rapid diagnosis and the identification of pathogenic v. parahaemolyticus are very important in the context of public health. in this study, an evagreen-based multiplex real-time pcr assay was established for the detection of pathogenic v. parahaemolyticus. this assay targeted three genetic markers of v. parahaemolyticus (species-specific gene toxr and virulence genes tdh a ... | 2014 | 24924797 |
| visualized analysis of cellular fatty acid profiles of vibrio parahaemolyticus strains under cold stress. | vibrio parahaemolyticus is a common foodborne bacterial pathogen, which survives in cold environments and is sometimes difficult to culture. fatty acid analysis under cold stress was conducted for several v. parahaemolyticus strains using gas chromatography/mass spectrometry, and the results were compared with those of the controls. all the fatty acid profiles obtained were visualized by multidimensional scaling (mds) and self-organized map (som). it was observed that the fatty acid profiles of ... | 2014 | 24910303 |
| the effects of storage temperature on the growth of vibrio parahaemolyticus and organoleptic properties in oysters. | during harvesting and storage, microbial pathogens and natural spoilage flora may grow, negatively affecting the composition and texture of oysters and posing a potential health threat to susceptible consumers. a solution to these problems would mitigate associated damaging effects on the seafood industry. the purpose of this study was to investigate the effects of storage temperature on growth of vibrios as well as other microbial, sensory, and textural characteristics of post-harvest shellstoc ... | 2014 | 24904911 |
| proteome profile of a pandemic vibrio parahaemolyticus sc192 strain in the planktonic and biofilm condition. | vibrio parahaemolyticus is one of the leading causative agents of foodborne diseases in humans. in this study, the proteome profiles of the pandemic strain v. parahaemolyticus sc192 belonging to the o3:k6 serovar during the planktonic and biofilm stages were analyzed by two-dimensional liquid chromatography coupled to tandem mass spectrometry. this non-gel-based multidimensional protein identification technology approach identified 45.5% of the proteome in the reference genome v. parahaemolyticu ... | 2014 | 24856245 |
| impact of hurricane irene on vibrio vulnificus and vibrio parahaemolyticus concentrations in surface water, sediment, and cultured oysters in the chesapeake bay, md, usa. | to determine if a storm event (i.e., high winds, large volumes of precipitation) could alter concentrations of vibrio vulnificus and v. parahaemolyticus in aquacultured oysters (crassostrea virginica) and associated surface water and sediment, this study followed a sampling timeline before and after hurricane irene impacted the chesapeake bay estuary in late august 2011. aquacultured oysters were sampled from two levels in the water column: surface (0.3 m) and near-bottom (just above the sedimen ... | 2014 | 24847319 |
| a novel whole genome amplification method using type iis restriction enzymes to create overhangs with random sequences. | ligation-mediated polymerase chain reaction (lm-pcr) is a whole genome amplification (wga) method, for which genomic dna is cleaved into numerous fragments and then all of the fragments are amplified by pcr after attaching a universal end sequence. however, the self-ligation of these fragments could happen and may cause biased amplification and restriction of its application. to decrease the self-ligation probability, here we use type iis restriction enzymes to digest genomic dna into fragments ... | 2014 | 24833422 |
| suitability of the molecular subtyping methods intergenic spacer region, direct genome restriction analysis, and pulsed-field gel electrophoresis for clinical and environmental vibrio parahaemolyticus isolates. | vibrio parahaemolyticus is the leading cause of infectious illness associated with seafood consumption in the united states. molecular fingerprinting of strains has become a valuable research tool for understanding this pathogen. however, there are many subtyping methods available and little information on how they compare to one another. for this study, a collection of 67 oyster and 77 clinical v. parahaemolyticus isolates were analyzed by three subtyping methods--intergenic spacer region (isr- ... | 2014 | 24799175 |
| a quantitative risk assessment model for vibrio parahaemolyticus in raw oysters in sao paulo state, brazil. | a risk assessment of vibrio parahaemolyticus associated with raw oysters produced and consumed in são paulo state was developed. the model was built according to the united states food and drug administration framework for risk assessment. the outcome of the exposure assessment estimated the prevalence and density of pathogenic v. parahaemolyticus in raw oysters from harvest to consumption. the result of the exposure step was combined with a beta-poisson dose-response model to estimate the proba ... | 2014 | 24786920 |
| trends in the epidemiology of pandemic and non-pandemic strains of vibrio parahaemolyticus isolated from diarrheal patients in kolkata, india. | a total of 178 strains of v. parahaemolyticus isolated from 13,607 acute diarrheal patients admitted in the infectious diseases hospital, kolkata has been examined for serovar prevalence, antimicrobial susceptibility and genetic traits with reference to virulence, and clonal lineages. clinical symptoms and stool characteristics of v. parahaemolyticus infected patients were analyzed for their specific traits. the frequency of pandemic strains was 68%, as confirmed by group-specific pcr (gs-pcr). ... | 2014 | 24786538 |
| specific detection of vibrio parahaemolyticus by fluorescence quenching immunoassay based on quantum dots. | in this study, anti-vibrio parahaemolyticus polyclonal and monoclonal antibodies were prepared through intradermal injection immune and lymphocyte hybridoma technique respectively. cdte quantum dots (qds) were synthesized at ph 9.3, 98 °c for 1 h with stabilizer of 2.7:1. the fluorescence intensity was 586.499, and the yield was 62.43%. qd probes were successfully prepared under the optimized conditions of ph 7.4, 37 °c for 1 h, 250 μl of 50 mg/ml edc · hcl, 150 μl of 4 mg/ml nhs, buffer system ... | 2014 | 24756606 |
| increase in vibrio parahaemolyticus infections associated with consumption of atlantic coast shellfish--2013. | vibrio parahaemolyticus (vp) is found naturally in coastal saltwater. in the united states, vp causes an estimated 35,000 domestically acquired foodborne infections annually, of which most are attributable to consumption of raw or undercooked shellfish. illness typically consists of mild to moderate gastroenteritis, although severe infection can occur. demographic, clinical, and exposure information (including traceback information on implicated seafood) for all laboratory-confirmed illnesses ar ... | 2014 | 24739344 |
| fate of vibrio parahaemolyticus on shrimp after acidic electrolyzed water treatment. | the objective of this study was to investigate the fate of vibrio parahaemolyticus on shrimp after acidic electrolyzed water (aew) treatment during storage. shrimp, inoculated with a cocktail of four strains of v. parahaemolyticus, were stored at different temperatures (4-30 °c) after aew treatment. experimental data were fitted to modified gompertz and log-linear models. the fate of v. parahaemolyticus was determined based on the growth and survival kinetics parameters (lag time, λ; the maximum ... | 2014 | 24727382 |
| draft genome sequences of six strains of vibrio parahaemolyticus isolated from early mortality syndrome/acute hepatopancreatic necrosis disease shrimp in thailand. | some strains of vibrio parahaemolyticus cause acute hepatopancreatic necrosis disease (ahpnd) in shrimp. we sequenced 3 ahpnd and 3 non-ahpnd strains and found that all of them lacked the pathogenicity island relevant to human infection. a unique sequence encoding a type iv pilus/type iv secretion system was found in 3 ahpnd strains. | 2014 | 24723705 |
| [analysis of molecular features of clinical vibrio parahaemolyticus strains in china]. | to explore the phenetic and genetic features of clinical vibrio parahaemolyticus strains from 2007-2009 in china. | 2014 | 24713290 |
| prevalence of urease in vibrio parahaemolyticus from the mississippi sound. | vibrio parahaemolyticus is a pathogenic marine bacterium that causes food-borne gastroenteritis and, less commonly, wound infections. as is the case for many pathogens, all v. parahaemolyticus strains possess at least one erythrocyte-lysing haemolysin. in addition, many v. parahaemolyticus also possess the enzyme urease. we tested 206 environmental v. parahaemolyticus isolates from mississippi coastal waters for urease and haemolytic activity using urea agar with added salt and wagatsuma agar, r ... | 2014 | 24698333 |
| characterization of antimicrobial resistance of vibrio parahaemolyticus from cultured sea cucumbers (apostichopus japonicas). | this study was aimed to evaluate the antimicrobial resistance and molecular resistance mechanisms of 87 vibrio parahaemolyticus isolates from cultured sea cucumbers (apostichopus japonicus). the results showed that all isolates were resistant to ampicillin and cephazolin, fewer of them were resistant to streptomycin (43·7%), cefuroxime sodium (18·4%), tetracycline (4·6%), sulphamethoxazole/trimethoprim (2·3%) and four quinolones (2·3%). more than half (56·2%) of the isolates displayed multiple r ... | 2014 | 24684348 |
| pandemic vibrio parahaemolyticus, maryland, usa, 2012. | | 2014 | 24655659 |
| vibrio parahaemolyticus, southern coastal region of china, 2007-2012. | we analyzed the prevalence and characteristics of vibrio parahaemolyticus among patients with acute infectious diarrhea in the southern coastal region of china. v. parahaemolyticus was the leading cause of bacterial infectious diarrhea in this region during 2007-2012. serotype o3:k6 strains were most common, followed by serotypes o4:k8 and o3:k29. | 2014 | 24655369 |
| complete genome sequence of vibrio parahaemolyticus environmental strain ucm-v493. | vibrio parahaemolyticus is the leading bacterial cause of seafood-related gastroenteritis in the world. here, we report the complete genome sequence and annotation of an environmental strain of v. parahaemolyticus, ucm-v493, with the aim of understanding the differences between the clinical and environmental isolates of the bacteria. we also make some preliminary sequence comparisons with the clinical strain rimd2210633. | 2014 | 24625868 |
| overexpression of vmetuv encoding a multidrug efflux transporter of vibrio parahaemolyticus causes bile acid resistance. | we isolated deoxycholate-resistant mutants from vibrio parahaemolyticus rtm34, which lacks four multidrug efflux transporters belonging to the resistance nodulation cell division (rnd) family. rtm34 showed sensitivity to many antimicrobial agents such as cholate and deoxycholate. deoxycholate-resistant mutants from rtm34 have elevated resistance to not only deoxycholate, but also antibiotics, disinfectants, and dyes. rt-pcr analysis revealed that the expression of vmev, which encodes an rnd-type ... | 2014 | 24613752 |
| identification of vpa0451 as the specific chaperone for the vibrio parahaemolyticus chromosome 1 type iii-secreted effector vpa0450. | vibrio parahaemolyticus is an important cause of gastroenteritis resulting from the consumption of raw or undercooked shellfish. the v. parahaemolyticus genome revealed the presence of two type iii secretion systems (t3ss); one on each of the two chromosomes. to date, four effectors have been identified as secreted by the chromosome 1 t3ss (t3ss1). for some effectors, efficient secretion requires a cytosolic chaperone that is often encoded in close proximity to its cognate effector. in this stud ... | 2014 | 24606036 |
| draft genome sequence of vibrio parahaemolyticus strain m0605, which causes severe mortalities of shrimps in mexico. | acute hepatopancreatic necrosis disease (ahpnd), also known as early mortality syndrome (ems), causes high mortalities in cultured shrimps in asia (l. tran et al., dis. aquat. organ. 105:45-55, 2013, http://dx.doi.org/10.3354/dao02621). here, we report the draft genome sequence of one mexican strain of vibrio parahaemolyticus that causes similar clinical signs in diseased shrimps. | 2014 | 24604636 |
| antimicrobial susceptibility of vibrio vulnificus and vibrio parahaemolyticus recovered from recreational and commercial areas of chesapeake bay and maryland coastal bays. | vibrio vulnificus and v. parahaemolyticus in the estuarine-marine environment are of human health significance and may be increasing in pathogenicity and abundance. vibrio illness originating from dermal contact with vibrio laden waters or through ingestion of seafood originating from such waters can cause deleterious health effects, particularly if the strains involved are resistant to clinically important antibiotics. the purpose of this study was to evaluate antimicrobial susceptibility among ... | 2014 | 24586914 |
| molecular diversity and predictability of vibrio parahaemolyticus along the georgian coastal zone of the black sea. | vibrio parahaemolyticus is a leading cause of seafood-related gastroenteritis and is also an autochthonous member of marine and estuarine environments worldwide. one-hundred seventy strains of v. parahaemolyticus were isolated from water and plankton samples collected along the georgian coast of the black sea during 28 months of sample collection. all isolated strains were tested for presence of tlh, trh, and tdh. a subset of strains were serotyped and tested for additional factors and markers o ... | 2014 | 24575085 |
| occurrence and distribution of vibrio vulnificus and vibrio parahaemolyticus--potential roles for fish, oyster, sediment and water. | vibrio vulnificus and vibrio parahaemolyticus are gram-negative marine bacteria naturally found in estuaries such as the gulf of mexico and can be pathogenic to humans. we quantified both of these organisms in fish, oyster, sediment, and water using culture-independent (quantitative pcr; qpcr) and culture-dependent (direct plating-colony hybridization; dp-ch) techniques during the transition period between winter and spring. we correlated these levels to environmental conditions and to abundance ... | 2014 | 24571291 |
| bacteriophage therapy of a vibrio parahaemolyticus infection caused by a multiple-antibiotic-resistant o3:k6 pandemic clinical strain. | recently isolated vibrio parahaemolyticus strains have displayed multiple antibiotic resistance. alternatives to conventional antibiotics are needed, especially for the multiple-antibiotic-resistant v. parahaemolyticus pandemic strain. | 2014 | 24558119 |
| genotype to phenotype: identification of diagnostic vibrio phenotypes using whole genome sequences. | vibrios are ubiquitous in the aquatic environment and can be found in association with animal or plant hosts. the range of ecological relationships includes pathogenic and mutualistic associations. to gain a better understanding of the ecology of these microbes, it is important to determine their phenotypic features. however, the traditional phenotypic characterization of vibrios has been expensive, time-consuming and restricted in scope to a limited number of features. in addition, most of the ... | 2014 | 24505074 |
| isolation and identification of vibrio parahaemolyticus from seawater and sediment samples in the southern coast of the caspian sea. | the objectives of this study were to investigate the occurrence of vibrio parahaemolyticus in the seawater and its sediment by molecular techniques and conventional microbiological methods. of 300 samples analyzed, 20.3 % was recorded positive for v. parahaemolyticus. of the 62 strains isolated, 26 (8.3 %) were obtained from the seawater samples, and 36 (12 %); from sediments. only three strains (4.83 %) showed hemolytic activity in wagatsuma agar. the results of this study demonstrated the pres ... | 2014 | 24482636 |
| rapid detection of vibrio parahaemolyticus in raw oysters using immunomagnetic separation combined with loop-mediated isothermal amplification. | the objective of this study was to develop a method that combined nanoparticle-based immunomagnetic separation (ims) with real-time loop-mediated isothermal amplification (lamp) for the rapid detection of vibrio parahaemolyticus. magnetic nanoparticles were functionalized with monoclonal antibodies that were produced against flagella from v. parahaemolyticus to capture and separate the target cells from raw oysters. after optimization, the immunomagnetic nanoparticles (imnps) presented a capture ... | 2014 | 24480190 |
| the trend of vibrio parahaemolyticus infections in southern thailand from 2006 to 2010. | the bacterium, vibrio parahaemolyticus was isolated from 776 patients at hat yai hospital in southern thailand from 2006 to 2010. 51.3-73.6% of the isolates were tdh (+) trh (-) and group-specific pcr positive pandemic strains. a comparison of the number of v. parahaemolyticus isolates in this study and that from the same hospital in 2000-2005 indicates that this region of thailandis endemic for v. parahaemolyticus. | 2013 | 24478592 |
| loss of sigma factor rpon increases intestinal colonization of vibrio parahaemolyticus in an adult mouse model. | vibrio parahaemolyticus is the leading cause of bacterial seafood-borne gastroenteritis worldwide, yet little is known about how this pathogen colonizes the human intestine. the alternative sigma factor rpon/sigma-54 is a global regulator that controls flagellar synthesis, as well as a wide range of nonflagellar genes. we constructed an in-frame deletion mutation in rpon (vp2670) in v. parahaemolyticus rimd2210633, a clinical serogroup o3:k6 isolate, and examined the effects in vivo using a stre ... | 2014 | 24478070 |
| proteomic identification of responsive proteins of vibrio parahaemolyticus under high hydrostatic pressure. | high hydrostatic pressure (hhp) processing is currently being used as a treatment for certain foods to inhibit spoilage organisms and control the presence of foodborne pathogens. in this study proteome profiles were performed by two-dimensional gel electrophoresis (2-de) coupled with maldi-tof/tof identification to determine the effects of hhp (50, 100, 150 and 200 mpa, each for 10 min) on vibrio parahaemolyticus atcc 17802 (∼8 log cfu ml⁻¹) in order to understand how it responds to mechanical s ... | 2014 | 24473993 |
| [macrophage migration inhibitory factor in mud crab scylla paramamosain: molecular cloning, expression profiles in various tissues and under vibrio challenge]. | as one of the first found cytokines, macrophage migration inhibitory factor (mif) plays an important role in several physiological processes in crabs. in this study, a full-length mif cdna (genbank accession number: jx131610) from mud crab scylla paramamosain (sp) was cloned based on a sequence of s. paramamosain cdna library. the full length of spmif was 734 bp consisting of a 363 bp open reading frame encoding the spmif, a 120 amino acid peptide chain. the molecular weight of spmif was 13.46 k ... | 2014 | 24466752 |
| [establishment of a method for gene complementation in vibrio parahaemolyticus]. | to establish a method for gene complementation in vibrio parahaemolyticus using the plasmid pbad33. | 2014 | 24463120 |
| vibrio parahaemolyticus induced necrotizing fasciitis: an atypical organism causing an unusual presentation. | background necrotizing fasciitis (nf) represents a life-threatening bacterial infection characterized by a rapid necrosis of deep subcutaneous tissue and facia underlying the skin. despite its lethal nature, nf occurs infrequently, leaving many physicians unfamiliar with the disease process, common pathogens, and treatment strategies. here we present a case of nf caused by an unlikely organism, vibrio parahaemolyticus. we highlight the innocuous nature of initial presentation and the potentially ... | 2013 | 24455339 |
| phenotypic and genotypic characterization of canadian clinical isolates of vibrio parahaemolyticus collected from 2000 to 2009. | vibrio parahaemolyticus is the leading bacterial cause of food-borne illness due to the consumption of contaminated seafood. the aim of the present study was to determine the population of its subtypes and establish a better understanding of the various types of v. parahaemolyticus strains that are causing human illness in canada. the subtypes for 100 human clinical isolates of v. parahaemolyticus collected between 2000 and 2009 were determined by performing serotyping, ribotyping, pulsed-field ... | 2014 | 24452166 |
| application of the vpp1 bacteriophage combined with a coupled enzyme system in the rapid detection of vibrio parahaemolyticus. | for rapid and quantitative detection of vibrio parahaemolyticus, a method combining the specific lysis of bacteriophages with a bacterial luciferase-flavin mononucleotide:nicotinamide adenine dinucleotide oxidoreductase bioluminescent system in vitro was developed. a v. parahaemolyticus detection system was established by optimizing three main influencing factors: bacteriophage titer, volume ratio of the bacteriophage to its host bacterium, and lysis time. a standard curve between the number of ... | 2014 | 24440165 |
| pandemic vibrio parahaemolyticus o3:k6 on the american continent. | vibrio parahaemolyticus is one of the most important seafood-borne bacterial in recent years and is the leading causal agent of human acute gastroenteritis, primarily following the consumption of raw, undercooked or mishandled marine products. until 1996, infections caused by v. parahaemolyticus were generally associated with diverse serovars. however, in february 1996, a unique serovar (o3:k6) of v. parahaemolyticus with specific genetic markers (tdh, toxrs/new and/or orf8) appeared abruptly in ... | 2014 | 24427744 |
| effect of acid adaptation on the environmental stress tolerance of three strains of vibrio parahaemolyticus. | three strains of vibrio parahaemolyticus (690, bcrc 13023, and bcrc 13025), involved in foodborne outbreaks in taiwan, were subjected to acid adaptation at ph 5.5 for 90 min. the effects of acid adaptation on the tolerance of v. parahaemolyticus to various environmental stresses, including heat (47°c), cold (4°c and -20°c), ethanol (8%), high salt (20% nacl), and hydrogen peroxide (20 ppm) were examined. results showed that acid adaptation increased the thermal tolerance of the three test strain ... | 2014 | 24410096 |
| multiple enzymatic profiles of vibrio parahaemolyticus strains isolated from oysters. | the enzymatic characterization of vibrios has been used as a virulence indicator of sanitary interest. the objective of this study was to determine the enzymatic profile of vibrio parahaemolyticus strains (n=70) isolated from crassostrea rhizophorae oysters. the strains were examined for the presence of gelatinase (gel), caseinase (cas), elastase (elas), phospholipase (phos), lipase (lip), amilase (aml) and dnase. all enzymes, except elastase, were detected in more than 60% of the strains. the m ... | 2014 | 24401782 |
| genetic characterization of trh positive vibrio spp. isolated from norway. | the thermostable direct hemolysin (tdh) and/or tdh-related hemolysin (trh) genes are carried by most virulent vibrio parahaemolyticus serovars. in norway, trh+ v. parahaemolyticus constitute 4.4 and 4.5% of the total number of v. parahaemolyticus isolated from blue mussel (mytilus edulis) and water, respectively. the trh gene is located in a region close to the gene cluster for urease production (ure). this region was characterized in v. parahaemolyticus strain th3996 and it was found that a nic ... | 2013 | 24400227 |
| experimental infection of brown-marbled grouper, epinephelus fuscoguttatus (forskal), with vibrio parahaemolyticus identifies parvalbumin beta-2 subunit i, alpha-2-macroglobulin, nattectin and immunoglobulin light chain, differentially expressed in resistant grouper. | the mechanisms through which brown-marbled grouper accomplishes resistance to infection, particularly against vibrios, are not yet fully understood. in this study, brown-marbled grouper fingerlings were experimentally infected with vibrio parahaemolyticus, to identify disease resistance grouper, and the serum proteome profiles were compared between resistant and susceptible candidates, via two-dimensional gel electrophoresis (2-de). the results showed that putative parvalbumin beta-2 subunit i, ... | 2015 | 24397626 |
| distribution and dynamics of epidemic and pandemic vibrio parahaemolyticus virulence factors. | vibrio parahaemolyticus, autochthonous to estuarine, marine, and coastal environments throughout the world, is the causative agent of food-borne gastroenteritis. more than 80 serotypes have been described worldwide, based on antigenic properties of the somatic (o) and capsular (k) antigens. serovar o3:k6 emerged in india in 1996 and subsequently was isolated worldwide, leading to the conclusion that the first v. parahaemolyticus pandemic had taken place. most strains of v. parahaemolyticus isola ... | 2013 | 24377090 |
| presence of t3ss2β genes in trh⁺ vibrio parahaemolyticus isolated from seafood harvested along mangalore coast, india. | vibrio parahaemolyticus is a seafood-borne pathogen autochthonous to the marine and estuarine ecosystem, responsible for gastroenteritis when contaminated raw seafood is consumed. the pathogenicity has been associated with thermostable direct haemolysin (tdh) and tdh-related haemolysin (trh). of late, the presence of t3ss2α and t3ss2β gene clusters has been well documented in clinical isolates of vibrio parahaemolyticus and known to play an essential role in pathogenesis. however, reports on the ... | 2014 | 24372411 |
| [antimicrobial resistance and virulence characteristics of vibrio parahaemolyticus]. | to explore drug resistance and virulence characteristics of clinically isolated vibrio parahaemolyticus (vp). | 2013 | 24360109 |
| the vibrio parahaemolyticus effector vopc mediates cdc42-dependent invasion of cultured cells but is not required for pathogenicity in an animal model of infection. | vibrio parahaemolyticus is a gram-negative marine bacterium that causes acute gastroenteritis in humans. the virulence of v. parahaemolyticus is dependent upon a type iii secretion system (t3ss2). one effector for t3ss2, vopc, is a homologue of the catalytic domain of cytotoxic necrotizing factor (cnf), and was recently reported to be a rho family gtpase activator and to be linked to internalization of v. parahaemolyticus by non-phagocytic cultured cells. here, we provide direct evidence that vo ... | 2014 | 24345190 |
| genome anatomy of the gastrointestinal pathogen, vibrio parahaemolyticus of crustacean origin. | vibrio parahaemolyticus, an important human pathogen, is associated with gastroenteritis and transmitted through partially cooked seafood. it has become a major concern in the production and trade of marine food products. the prevalence of potentially virulent and pathogenic v. parahaemolyticus in raw seafood is of public health significance. here we describe the genome sequence of a v. parahaemolyticus isolate of crustacean origin which was cultured from prawns in 2008 in selangor, malaysia (is ... | 2013 | 24330647 |
| seasonal effects of heat shock on bacterial populations, including artificial vibrio parahaemolyticus exposure, in the pacific oyster, crassostrea gigas. | during the warmer summer months, oysters are conditioned to spawn, resulting in massive physiological efforts for gamete production. moreover, the higher temperatures during the summer typically result in increased bacteria populations in oysters. we hypothesized that these animals are under multiple stresses that lead to possible immune system impairments during the summer months that can possibly lead to death. here we show that in the summer and the fall animals exposed to a short heat stress ... | 2014 | 24290632 |
| molecular characterization, phylogeny and expression of a hepcidin gene in the blotched snakehead channa maculata. | a hepcidin-like gene (cmhep) was cloned and characterized from the liver of the blotched snakehead channa maculata. the complete cmhep cdna was 756 bp in length, containing an open reading frame of 270 bp (encoding 89 amino acids), flanked by 210 bp and 276 bp of 5' and 3' untranslated regions, respectively. the deduced peptide of 89 amino acids consisted of 24 aa, 40 aa and 25 aa for signal peptide, prodomain and mature peptide, respectively. the mature peptide had eight cysteines at the identi ... | 2014 | 24287270 |
| a dual-color flow cytometry protocol for the simultaneous detection of vibrio parahaemolyticus and salmonella typhimurium using aptamer conjugated quantum dots as labels. | a sensitive, specific method for the collection and detection of pathogenic bacteria was demonstrated using quantum dots (qds) as a fluorescence marker coupled with aptamers as the molecular recognition element by flow cytometry. the aptamer sequences were selected using a bacterium-based selex strategy in our laboratory for vibrio parahaemolyticus and salmonella typhimurium that, when applied in this method, allows for the specific recognition of the bacteria from complex mixtures including shr ... | 2013 | 24267076 |
| vibrio vulnificus bacteriophage ssp002 as a possible biocontrol agent. | a novel vibrio vulnificus-infecting bacteriophage, ssp002, belonging to the siphoviridae family, was isolated from the coastal area of the yellow sea of south korea. host range analysis revealed that the growth inhibition of phage ssp002 is relatively specific to v. vulnificus strains from both clinical and environmental samples. in addition, a one-step growth curve analysis and a bacteriophage stability test revealed a latent period of 65 min, a burst size of 23 ± 2 pfu, as well as broad temper ... | 2014 | 24212569 |
| translocation of vibrio parahaemolyticus across an in vitro m cell model. | consumption of vibrio parahaemolyticus via contaminated shellfish results in inflammatory gastroenteritis characterised by severe diarrhoea, nausea and stomach cramps. this study investigated the translocation of v. parahaemolyticus across a peyer's patch m cell-like caco-2/raji b co-culture model system, as m cells represent a primary site of infection for many pathogenic bacteria. vibrio parahaemolyticus translocated across co-culture monolayers in higher numbers as compared to caco-2 monolaye ... | 2014 | 24188736 |
| identification of a molecular signature unique to metal-reducing gammaproteobacteria. | functional genes required for microbial (dissimilatory) metal reduction display high sequence divergence, which limits their utility as molecular biomarkers for tracking the presence and activity of metal-reducing bacteria in natural and engineered systems. in the present study, homologs of the outer membrane beta-barrel protein mtrb of metal-reducing gammaproteobacteria were found to contain a unique n-terminal cxxc motif that was missing from mtrb homologs of nonmetal-reducing gammaproteobacte ... | 2014 | 24188569 |
| defining the niche of vibrio parahaemolyticus during pre- and post-monsoon seasons in the coastal arabian sea. | the bacteria vibrio parahaemolyticus is an important component of coastal ecosystems worldwide, and in recent years, v. parahaemolyticus has caused several cases of food-borne gastroenteritis. however, research investigating which parameters are important in regulating v. parahaemolyticus abundance in tropical areas with relatively stable temperatures and salinity are largely lacking. the objective here was to investigate which environmental forces are driving elevated abundances of v. parahaemo ... | 2014 | 24158690 |
| contamination by vibrio parahaemolyticus and its virulent strains in seafood marketed in thailand, vietnam, malaysia, and indonesia. | infections by virulent strains of vibrio parahaemolyticus are frequently reported in southeast asia. this is due to the frequent seafood contamination by virulent strains. in this study conducted from 2008 to 2011, seafood like fish, shrimp, squid, crab, and molluscan shellfish were purchased from provinces in thailand and three southeast asian countries and examined for the prevalence of three genetic markers of v. parahaemolyticus (species-specific gene: toxr gene, virulence genes: tdh and trh ... | 2013 | 24155650 |
| molecular characterisation of a multidrug resistance conjugative plasmid from vibrio parahaemolyticus. | vibrio parahaemolyticus is a major causative agent of gastroenteritis and is the leading cause of food-borne illness in hong kong. recent studies of resistance to extended-spectrum β-lactams and fluoroquinolones in v. parahaemolyticus have caused huge concern. this work reports the characterisation of a multidrug resistance conjugative plasmid in v. parahaemolyticus isolated from shrimp samples from hong kong. the plasmid is ca. 200 kb and carries multidrug resistance genes, including a novel pl ... | 2013 | 24139885 |
| epidemiology and etiology characteristics of foodborne outbreaks caused by vibrio parahaemolyticus during 2008-2010 in guangdong province, china. | vibrio parahaemolyticus infection has been considered the leading cause of bacterial illnesses mainly associated with seafood consumption in guangdong province in china. in this study, epidemiological and etiological characteristics of 36 v. parahaemolyticus outbreaks that occurred from 2008 to 2010 in guangdong province were analyzed; 284 strains involved were characterized by serotyping; virulence genes and 66 strains from four outbreaks therein were subtyped by pulsed-field gel electrophoresi ... | 2014 | 24138080 |
| a predictive model for assessment of decontamination effects of lactic acid and chitosan used in combination on vibrio parahaemolyticus in shrimps. | vibrio parahaemolyticus is a major causative agent of human gastroenteritis in seafood products including shrimps. lactic acid and chitosan are natural antimicrobials for food decontamination in the washing process of seafood. in this research, a 4-factor response surface model based on the box-behnken experimental design was developed to evaluate the effects of lactic acid (1%, 2%, and 3%, v/v), chitosan (0.4%, 1%, and 1.6%, w/v), rotational rate (90, 110, and 130 rpm) and washing time (10, 20, ... | 2013 | 24135668 |
| spread of pacific northwest vibrio parahaemolyticus strain. | | 2013 | 24131194 |
| [molecular characterization of vibrio parahaemolyticus collected from human infections in shenzhen, between 2002 and 2008]. | to determine the occurrence and distribution of specific clones of pathogenic vibrio parahaemolyticus(vp)isolated in shenzhen and to assess the relationship between serotype o3:k6 and the globally distributed pandemic clone. | 2013 | 24125615 |
| the bacterial effector vopl organizes actin into filament-like structures. | vopl is an effector protein from vibrio parahaemolyticus that nucleates actin filaments. vopl consists of a vopl c-terminal domain (vcd) and an array of three wasp homology 2 (wh2) motifs. here, we report the crystal structure of the vcd dimer bound to actin. the vcd organizes three actin monomers in a spatial arrangement close to that found in the canonical actin filament. in this arrangement, wh2 motifs can be modeled into the binding site of each actin without steric clashes. the data suggest ... | 2013 | 24120140 |
| fatty acid profiles of vibrio parahaemolyticus and its changes with environment. | the fatty acid (fa) profiles of 15 strains representing four genera (vibrio, pseudomonas, aeromonas, and shewanella) and seven species were compared by capillary gas chromatography with flame ionization detection. fa fingerprints of vibrio parahaemolyticus were established by similarity calculation and principle component analysis. this provided a simple measure for distinguishing v. parahaemolyticus from other bacteria. the similarity scores indicated by correlation coefficient and vector cosin ... | 2015 | 24114713 |
| modeling the thermoultrasound inactivation of vibrio parahaemolyticus in raw peeled shrimps. | vibrio parahaemolyticus has been identified as a causative agent for seafoodborne diseases worldwide. the effect of thermoultrasound treatment on the survival of v. parahaemolyticus in raw peeled shrimps was investigated in this study as an alternative bacterial inactivation method in seafood as part of the postharvest washing process. raw peeled shrimps inoculated with v. parahaemolyticus were treated with mild heat (47, 50, and 53°c) combined with ultrasound (0, 96, 150, and 204 w) based on a ... | 2013 | 24112570 |
| characteristic and functional analysis of toll-like receptors (tlrs) in the lophotrocozoan, crassostrea gigas, reveals ancient origin of tlr-mediated innate immunity. | the evolution of tlr-mediated innate immunity is a fundamental question in immunology. here, we report the characterization and functional analysis of four tlr members in the lophotrochozoans crassostreagigas (cgtlrs). all cgtlrs bear a conserved domain organization and have a close relationship with tlrs in ancient non-vertebrate chordates. in hek293 cells, every cgtlr could constitutively activate nf-κb responsive reporter, but none of the pamps tested could stimulate cgtlr-activated nf-κb ind ... | 2013 | 24098508 |
| the syp enhancer sequence plays a key role in transcriptional activation by the σ54-dependent response regulator sypg and in biofilm formation and host colonization by vibrio fischeri. | biofilm formation by vibrio fischeri is a complex process that requires multiple regulators. one such regulator, the ntrc-like response regulator sypg, controls biofilm formation and host colonization by v. fischeri via its impact on transcription of the symbiosis polysaccharide (syp) locus. sypg is predicted to activate syp transcription by binding to the syp enhancer (se), a conserved sequence located upstream of four syp promoters. in this study, we performed an in-depth analysis of the seque ... | 2013 | 24097942 |
| potential use of chitosan nanoparticles for oral delivery of dna vaccine in black seabream acanthopagrus schlegelii bleeker to protect from vibrio parahaemolyticus. | to develop an effective and easy-to-administer vaccine against vibriosis of fish, the chitosan nanoparticles-loaded dna vaccine against vibrio parahaemolyticus was studied. a dna vaccine was constructed using the outer membrane protein k (ompk) gene of v. parahaemolyticus strain (os4) and pegfp-n2 , a eukaryotic expression vector, and the construct was named pegfp-n2 -ompk (pdna). the pdna was encapsulated in chitosan particles (chitosan/pdna). the effective diameter, mean diameter and polydispe ... | 2013 | 24093149 |
| antibiotic resistance and molecular typing among cockle (anadara granosa) strains of vibrio parahaemolyticus by polymerase chain reaction (pcr)-based analysis. | genomic dna of vibrio parahaemolyticus were characterized by antibiotic resistance, enterobacterial repetitive intergenic consensus-polymerase chain reaction (eric-pcr) and random amplified polymorphic dna-polymerase chain reaction (rapd-pcr) analysis. these isolates originated from 3 distantly locations of selangor, negeri sembilan and melaka (east coastal areas), malaysia. a total of 44 (n = 44) of tentatively v. parahaemolyticus were also examined for the presence of toxr, tdh and trh gene. o ... | 2014 | 24068534 |
| cell-free synthesis of functional thermostable direct hemolysins of vibrio parahaemolyticus. | vibrio parahaemolyticus is a recognized enteropathogen causing diarrhea in humans and is one of the major causes of seafoodborne gastroenteritis. an important virulence factor is thermostable direct hemolysin (tdh), a pore-forming toxin, which is able to lyse eukaryotic cells. the active toxin is a tetramer of four identical protein subunits, which is secreted by the pathogen after cleavage of a signal peptide. to establish diagnostic detection systems for tdh we expressed the hemolysin with and ... | 2013 | 24060377 |
| zebrafish as a model for vibrio parahaemolyticus virulence. | vibrio parahaemolyticus is a gram-negative, naturally occurring marine bacterium. subpopulations of strains belonging to this species cause an acute self-limiting gastroenteritis in humans and, less commonly, wound infections. in vivo models to differentiate avirulent and virulent strains and evaluate the pathogenic potential of strains of this species have been largely focused on the presence of known virulence factors such as the thermostable direct haemolysin (tdh), the tdh-related haemolysin ... | 2013 | 24056807 |
| association of a d-alanyl-d-alanine carboxypeptidase gene with the formation of aberrantly shaped cells during the induction of viable but nonculturable vibrio parahaemolyticus. | vibrio parahaemolyticus is a halophilic gram-negative bacterium that causes human gastroenteritis. when the viable but nonculturable (vbnc) state of this bacterium was induced by incubation at 4°c in morita minimal salt solution containing 0.5% nacl, the rod-shaped cells became coccoid, and various aberrantly shaped intermediates were formed in the initial stage. this study examined the factors that influence the formation of these aberrantly shaped cells. the proportion of aberrantly shaped cel ... | 2013 | 24056454 |
| quorum sensing modulates transcription of cpsq-mfpabc and mfpabc in vibrio parahaemolyticus. | vibrio parahaemolyticus apha and opar are the two master regulators of quorum sensing (qs) that are abundantly produced and operate at low cell density (lcd) and high cell density (hcd), respectively, with an outcome of reciprocally gradient production of these two proteins with transition between lcd and hcd. the cpsq-mfpabc gene cluster is transcribed as two operons cpsq-mfpabc and mfpabc in v. parahaemolyticus. mfpabc is a putative membrane fusion transporter that contributes to biofilm devel ... | 2013 | 24036587 |
| [study on the serotype, virulence genes and pulsed field gel electrophoresis molecular typing of vibrio parahaemolyticus isolated from clinical patients]. | to investigate the serotype virulence genes and molecular typing characteristics of vibrio parahaemolyticus isolated from diarrhea samples among 2007 -2012. | 2013 | 24024376 |
| evidences of snps in the variable region of hemocyanin ig-like domain in shrimp litopenaeus vannamei. | single nucleotide polymorphisms (snps) are the commonest mode of genetic variation in invertebrate immune-related genes. hemocyanin presents in the hemolymph of both mollusks and arthropods and functions as an important antigen non-specific immune protein. but people know very little about its gene polymorphism so far. in current study, bioinformatics, molecular biology and environmental challenge approaches were used to identify the snps within hemocyanin ig-like domain in shrimp litopenaeus va ... | 2013 | 24012752 |
| the sodium/iodide symporter: state of the art of its molecular characterization. | the sodium/iodide symporter (nis or slc5a5) is an intrinsic membrane protein implicated in iodide uptake into thyroid follicular cells. it plays a crucial role in iodine metabolism and thyroid regulation and its function is widely exploited in the diagnosis and treatment of benign and malignant thyroid diseases. a great effort is currently being made to develop a nis-based gene therapy also allowing the radiotreatment of nonthyroidal tumors. nis is also expressed in other tissues, such as saliva ... | 2014 | 23988430 |
| serotype, virulence, and genetic traits of foodborne and clinical vibrio parahaemolyticus isolates in shanghai, china. | vibrio parahaemolyticus is a major foodborne pathogen in china and other countries. in this study, a total of 578 clinical v. parahaemolyticus strains and 51 foodborne strains were isolated during the period from 2009 to 2011 in the eastern coastal city of shanghai, china. their serotypes, virulence genes, pandemic traits, and genotyping were investigated. a total of nine o groups and 20 k types were identified by serological analysis of all isolates. six different o groups and 14 different k ty ... | 2013 | 23988077 |
| in situ and in vitro impacts of the deepwater horizon oil spill on vibrio parahaemolyticus. | most established virulence genes in vibrio parahaemolyticus (vp), e.g., thermostable direct hemolysin (tdh), tdh-related hemolysin (trh), and type three secretion system 2 (ttss2), are on the chromosome 2 pathogenicity island, which also possesses numerous uncharacterized genes. we hypothesized the 2010 deepwater horizon (dh) oil spill would cause an increase in populations of vibrio parahaemolyticus carrying environmental adaptation genes. vp isolated pre- and post-spill were analyzed for ttss2 ... | 2013 | 23987095 |
| the msha pilus of vibrio parahaemolyticus has lectin functionality and enables ttss-mediated pathogenicity. | vibrio parahaemolyticus is a seafood-borne pathogen which causes acute inflammatory gastroenteritis--a process which is mediated by the translocation of type three secretion system effector proteins. the molecular interactions governing colonization of the intestinal epithelium by this pathogen remain poorly understood. the mannose-sensitive haemagglutinin (msha) pilus was identified in this study as a significant factor in bacterial-host cell adherence and subsequent pathogenesis towards caco-2 ... | 2013 | 23981476 |
| cell density- and quorum sensing-dependent expression of type vi secretion system 2 in vibrio parahaemolyticus. | vibrio parahaemolyticus apha and opar are the two master quorum sensing (qs) regulators that are abundantly expressed at low cell density (lcd) and high cell density (hcd), respectively, with a feature of reciprocally gradient production of them with transition between lcd and hcd. the type vi secretion system 2 (t6ss2) gene cluster can be assigned into three putative operons, namely vpa1027-1024, vpa1043-1028, and vpa1044-1046. t6ss2 contributes to adhesion of v. parahaemolyticus to host cells. | 2013 | 23977385 |
| [construction of biofilm formation related mutants in vibrio parahaemolyticus]. | to construct the mutants of biofilm related genes in vibrio parahaemolyticus and confirm the mutants. | 2013 | 23958128 |
| differences in the stress tolerances of vibrio parahaemolyticus strains due to their source and harboring of virulence genes. | to investigate the diversity of stress tolerance levels in vibrio parahaemolyticus, 200 v. parahaemolyticus strains isolated from various coastal environments, seafood, and human clinical cases were exposed to acid, low-osmolality, freezing-thawing, and heat stresses. tolerance against acid stress was higher in the virulent (tdh- and/or trh-positive) strains than in the avirulent (tdh- and trh-negative) strains. tolerance against low-osmolality, freezing-thawing, and heat stresses was higher in ... | 2013 | 23905806 |
| [preparation of monoclonal antibodies against flagellin core protein of vibrio parahaemolyticus and its activity analysis]. | to prepare monoclonal antibodies (mabs) against flagellin core protein of vibrio (v.) parahaemolyticus and establish the double-sandwich elisa for testing v.parahaemolyticus from food products. | 2013 | 23837985 |
| preliminary stochastic model for managing vibrio parahaemolyticus and total viable bacterial counts in a pacific oyster (crassostrea gigas) supply chain. | vibrio parahaemolyticus can accumulate and grow in oysters stored without refrigeration, representing a potential food safety risk. high temperatures during oyster storage can lead to an increase in total viable bacteria counts, decreasing product shelf life. therefore, a predictive tool that allows the estimation of both v. parahaemolyticus populations and total viable bacteria counts in parallel is needed. a stochastic model was developed to quantitatively assess the populations of v. parahaem ... | 2013 | 23834791 |
| [the development of complex technique of evaluation of virulence of parahemolytic vibrio]. | the article deals with results of studying parahemolytic vibrio separatedfrom different sources according their phenotype and genotype attributes associated with virulence. in certain cases the mismatch of results of kanagava tests and polymerase chain reaction test of gene tdh was established. the need in virulence complex evaluation is substantiated. this complex has to include detection of hemolytic activity in kanagava test and urease activity on the kristensen medium and polymerase chain re ... | 2013 | 23808010 |
| extensive investigation of antimicrobial resistance in vibrio parahaemolyticus from shellfish and clinical sources, italy. | | 2013 | 23796895 |
| draft genome sequence of vibrio parahaemolyticus v110, isolated from shrimp in hong kong. | we report the whole-genome sequence of a tdh- and trh-negative vibrio parahaemolyticus strain, v110, from shrimp. the major difference of v110 from clinical strains was its lack of the type iii secretion system t3ss2, a key component of virulence. further sequence comparison can shed light on the pathogenesis of v. parahaemolyticus. | 2013 | 23788537 |