| defence strategies and antibiotic resistance gene abundance in enterococci under stress by exposure to low doses of peracetic acid. | peracetic acid (paa) is an organic compound used efficiently as disinfectant in wastewater treatments. yet, at low doses it may cause selection; thus, the effect of low doses of paa on enterococcus faecium as a proxy of human-related microbial waste was evaluated. bacteria were treated with increasing doses of paa (from 0 to 25 mg l(-1) min) and incubated in regrowth experiments under non-growing, limiting conditions and under growing, favorable conditions. the changes in bacterial abundance, in ... | 2017 | 28715758 |
| first outbreak of colonization by linezolid- and glycopeptide-resistant enterococcus faecium harbouring the cfr gene in a uk nephrology unit. | to describe an outbreak of colonization by linezolid- and glycopeptide-resistant enterococcus faecium harbouring the cfr gene in a uk nephrology unit. | 2017 | 28698020 |
| characterization of high level ampicillin- and aminoglycoside-resistant enterococci isolated from non-hospital sources. | high level ampicillin- and aminoglycoside-resistant enterococci are being increasingly reported from non-hospital sources. this study was carried out to characterize these strains from non-hospital sources in nigeria. | 2017 | 28693665 |
| aging eye microbiota in dry eye syndrome in patients treated with enterococcus faecium and saccharomyces boulardii. | aging seem to have a key role in the onset and progression of ocular surface diseases. dry eye syndrome (des) is a multifactorial disease of the tears and ocular surface in which symptoms may interfere with the ability to work and carry out daily functions. | 2017 | 28676009 |
| naoh-hcl neutralized urine preparation for direct testing of uropathogens by bruker ms. | we evaluated the analytical performance of a naoh-hcl neutralization protocol for identifying uropathogens directly from urine samples by a bruker ms system (bruker daltonics, german) and sought to establish a protocol for integrating the method with conventional screening. | 2017 | 28649705 |
| enteric bacteria of food ice and their survival in alcoholic beverages and soft drinks. | this study aimed to evaluate the levels of enteric bacteria in ice cubes produced in different environments (home-made, prepared in bars and pubs with ice machines and produced in industrial plants) and to determine their survival in different alcoholic beverages and soft drinks. members of the enterobacteriaceae family were found in almost all samples analysed. all industrial and the majority of home-made samples did not contain coliforms. enterococci were not identified in domestic samples whi ... | 2017 | 28648289 |
| new and improved? a review of novel antibiotics for gram-positive bacteria. | the number of antibiotics in the pipeline targeting gram-positive pathogens has increased in recent years. | 2017 | 28642145 |
| novel vitamin b12-producing enterococcus spp. and preliminary in vitro evaluation of probiotic potentials. | vitamin b12 is an essential nutrient required for crucial metabolic processes in humans. vitamin b12-producing lactic acid bacteria (lab) have been attracting increased attentions currently because of the generally recognized as safe (gras) status. most of recent studies focused on lactobacillus, and little is known about b12-producing enterococcus. in the present study, five enterococcus strains isolated from infant feces were identified as vitamin b12 producers. among them, enterococcus faeciu ... | 2017 | 28634850 |
| time to review the gold standard for genotyping vancomycin-resistant enterococci in epidemiology: comparing whole-genome sequencing with pfge and mlst in three suspected outbreaks in sweden during 2013-2015. | vancomycin-resistant enterococci (vre) are a challenge to the health-care system regarding transmission rate and treatment of infections. vre outbreaks have to be controlled from the first cases which means that appropriate and sensitive genotyping methods are needed. the aim of this study was to investigate the applicability of whole genome sequencing based analysis compared to pulsed-field gel electrophoresis (pfge) and multi-locus sequence typing (mlst) in epidemiological investigations as we ... | 2017 | 28627467 |
| altered cytokine expression and barrier properties after in vitro infection of porcine epithelial cells with enterotoxigenic escherichia coli and probiotic enterococcus faecium. | the aim of the present study was to elucidate the effects of the probiotic feed additive enterococcus faecium ncimb 10415 (e. faecium) on porcine jejunal epithelial cells (ipec-j2) during an in vitro challenge with enterotoxigenic escherichia coli (etec). cells were incubated with e. faecium, etec, or both, and the effects on barrier function and structure and intra- and intercellular signaling were determined. coincubation with e. faecium abolished the etec-induced decrease in transepithelial r ... | 2017 | 28607532 |
| species distribution, antibiotic resistance and virulence traits in canine and feline enterococci in tunisia. | in order to investigate the possible role of dogs and cats in the carriage and potential dissemination of resistant enterococci, seventy faecal samples from dogs and cats were tested for enterococci. fifty-eight enterococci were recovered. isolates were identified as enterococcus faecium (n = 31) and e. faecalis (n = 14) e. durans (n = 6), e. casseliflavus (n = 2), e. hirae and e. gallinarum (2 isolates each). enterococcal isolates showed resistance to ciprofloxacin (n = 35), erythromycin (n = 3 ... | 2017 | 28605961 |
| lactic acid bacteria involved in cocoa beans fermentation from ivory coast: species diversity and citrate lyase production. | microbial fermentation is an indispensable process for high quality chocolate from cocoa bean raw material. lactic acid bacteria (lab) are among the major microorganisms responsible for cocoa fermentation but their exact role remains to be elucidated. in this study, we analyzed the diversity of lab in six cocoa producing regions of ivory coast. ribosomal 16s gene sequence analysis showed that lactobacillus plantarum and leuconostoc mesenteroides are the dominant lab species in these six regions. ... | 2017 | 28578265 |
| testing commercial biopreservative against spoilage microorganisms in map packed ricotta fresca cheese. | ricotta fresca cheese is susceptible to secondary contamination and is able to support the growth of pathogens or spoilage psychotrophic bacteria during storage. the aim of the present study was to evaluate which among three commercial biopreservatives was suitable to be used to control the growth of spoilage microorganisms in sheep's milk map ricotta fresca cheese. 144 ricotta fresca cheese samples were inoculated either with the bioprotective culture lyofast fpr 2 (including enterococcus faeci ... | 2017 | 28576375 |
| bacteriocin-producing enterococcus faecium lcw 44: a high potential probiotic candidate from raw camel milk. | bacterial isolates from raw camel milk were screened for antibacterial activity using the agar diffusion assay. ten isolates selected for their inhibition of gram-positive bacteria were identified by 16s sequencing as enterococcus faecium or durans. an isolate named e. faecium lcw 44 exhibited the broadest antibacterial spectrum with an inhibitory activity against several gram-positive strains belonging to the genera clostridium, listeria, staphylococcus, and lactobacillus. e. faecium lcw 44 was ... | 2017 | 28572793 |
| effects of enterococcus faecium slb 120 on growth performance, blood parameters, relative organ weight, breast muscle meat quality, excreta microbiota shedding, and noxious gas emission in broilers. | this 5-week study was conducted to determine the effects of enterococcus faecium (slb 120) on growth performance, blood parameters, relative organ weight, breast muscle meat quality, excreta microbiota shedding, and noxious gas emission in broilers. a total of 816 one-day-old male broilers were allocated to 4 groups with 12 replications (17 broilers/pen) according to body weight (43.2 ± 0.32 g). dietary treatment groups were: (1) con, basal diet, (2) t1, con + 0.05% e. faecium, (3) t2, con + 0.1 ... | 2017 | 28549196 |
| patients with community-acquired bacteremia of unknown origin: clinical characteristics and usefulness of microbiological results for therapeutic issues: a single-center cohort study. | bacteremia of unknown origin (buo) are associated with increased mortality compared to those with identified sources. microbiological data of those patients could help to characterize an appropriate empirical antibiotic treatment before bloodcultures results are available during sepsis of unknown origin. based on the dashboard of our ward that prospectively records several parameters from each hospitalization, we report 101 community-acquired buo selected among 1989 bacteremic patients from july ... | 2017 | 28526094 |
| the leaderless bacteriocin enterocin k1 is highly potent against enterococcus faecium: a study on structure, target spectrum and receptor. | enterocin k1 (entk1), enterocin ej97 (entej97), and lsbb are three sequence related leaderless bacteriocins. yet lsbb kills only lactococci while entk1 and entej97 target wider spectra with entk1 being particularly active against enterococcus faecium, including nosocomial multidrug resistant isolates. nmr study of entk1 showed that it had a structure very similar to lsbb - both having an amphiphilic n-terminal α-helix and an unstructured c-terminus. the α-helix in entk1 is, however, about 3-4 re ... | 2017 | 28515717 |
| contribution of type ii topoisomerase mutations to fluoroquinolone resistance in enterococcus faecium from japanese clinical setting. | high-level fluoroquinolone resistance is conferred by the mutation of conserved serine and acidic amino acids in the quinolone resistance-determining region (qrdr) of the a subunits of the type ii topoisomerases, dna gyrase (gyra) and topoisomerase iv (parc). in japan, fluoroquinolone-resistant enterococcus faecium continues to emerge in clinical settings. we analyzed 131 japanese e. faecium clinical isolates for susceptibility to levofloxacin (lvfx), and qrdr mutational status. the bacterial co ... | 2017 | 28504916 |
| activity of fosfomycin- and daptomycin-containing bone cement on selected bacterial species being associated with orthopedic infections. | the purpose of this study was to determine activity of fosfomycin/gentamicin and daptomycin/gentamicin-containing pmma bone-cement against staphylococcus aureus (mrsa, mssa), staphylococcus epidermidis, enterococcus faecium (vre), and e. coli (esbl; only fosfomycin). test specimens of the bone cement were formed and bacteria in two concentrations were added one time or repeatedly up to 96 h. all fosfomycin-containing cement killed ultimately all mssa, staphylococcus epidermidis, and e. coli with ... | 2017 | 28484708 |
| whole genome characterization of a naturally occurring vancomycin-dependent enterococcus faecium from a patient with bacteremia. | vancomycin-dependent enterococci are a relatively uncommon phenotype recovered in the clinical laboratory. recognition and recovery of these isolates are important, to provide accurate identification and susceptibility information to treating physicians. herein, we describe the recovery of a vancomycin-dependent and revertant e. faecium isolates harboring vanb operon from a patient with bacteremia. using whole genome sequencing, we found a unique single nucleotide polymorphism (s186n) in the d-a ... | 2017 | 28479127 |
| impact of faecal microbiota transplantation on mouse digestive colonization with two extensively resistant bacteria. | | 2017 | 28461230 |
| liver retransplantation for hepatic abscess due to hepatic artery thrombosis: a case report. | hepatic artery thrombosis (hat) is a well-recognized complication of liver transplantation (lt). hat is an important risk factor for infectious, in particular hepatic abscess, which can cause graft loss and increasing morbidity and mortality. | 2017 | 28457384 |
| [pathogen spectrum of complex wounds in germany from war zones]. | background treatment of the injured from war zones with wounds with multi-resistant pathogens is becoming more common in germany, especially in german armed forces hospitals. in most cases, the pre-treatment of these patients has been inadequate and they have a high load of rare pathogens. in germany, hospitals have to evaluate these pathogens by law. the aim of this study was to review current german guidelines and data on the array of pathogens of complex trauma wounds in germany and to compar ... | 2017 | 28423435 |
| an unusual location of gouty panniculitis: a case report. | gouty panniculitis, characterised by the deposition of monosodium urate crystals in subcutaneous tissue, is a rare clinical manifestation of gout. | 2017 | 28422890 |
| effects of dietary supplementation with a probiotic (enterococcus faecium dsm 7134) on growth performance, nutrient digestibility, and gut health status in weaning pigs. | probiotics have been suggested as desirable antibiotic alternatives to pigs by improving gut health and growth performance. however, limited studies of the effectiveness of e. faecium in weaning pigs, the objective of this study was to investigate the effects of e. faecium dsm 7134 supplementation on growth performance, nutrient digestibility, antioxidant parameters, volatile fatty acids concentration, intestinal histomorphology, and diarrhea score in weaning pig. | 2017 | 28403547 |
| ultraviolet (uv-c) inactivation of enterococcus faecium, salmonella choleraesuis and salmonella typhimurium in porcine plasma. | the objective of this study was to assess the effectiveness of an ultraviolet (uv-c, 254 nm) irradiation system on reducing the load of salmonella typhimurium (s. typhimurium), salmonella choleraesuis (s. choleraesuis) resistant to streptomycin and enterococcus faecium (e. faecium) inoculated in sterile porcine plasma and then subjected to different uv-c irradiation doses (750, 1500, 3000, 6000 and 9000 j/l) using a pilot plant uv-c device working under turbulent flow. results indicated that uv- ... | 2017 | 28399166 |