| differences in protein profiles of the isolates of entamoeba histolytica and e. dispar by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (seldi-tof ms) proteinchip assays. | surface-enhanced laser desorption ionization time of flight mass spectrometry (seldi-tof ms) proteinchip assays with weak cationic exchange chips were used for protein profiling of different isolates of entamoeba histolytica and e. dispar. when seldi-tof ms spectra of cell lysates from e. histolytica strain hm-1:imss were compared with those from four other laboratory strains (200:nih, hk-9, dkb, and saw755cr) grown under the same culture conditions, different peak patterns of seldi-tof ms were ... | 2007 | 17846790 |
| viability studies of pure carbon- and nitrogen-doped nanotubes with entamoeba histolytica: from amoebicidal to biocompatible structures. | | 2007 | 17849378 |
| [a case of amebic brain abscess]. | amebic brain abscess is a rare and usually fatal complication of entamoeba histolytica infections. we successfully treated a patient with this infection accompanied by brain, liver and pulmonary abscesses. treatment consisted of administration of metronidazole through a nasogastric tube, and drainage of the brain abscess. a 51-year-old male patient presented with a two-week history of fever, severe back pain, and chest pain. liver and pulmonary abscesses were drained upon admission. biopsy of th ... | 2007 | 17867313 |
| entamoeba histolytica: fibrilar aggregates in dividing trophozoites. | entamoeba histolytica trophozoite cytokinesis is dependent upon cytoskeletal elements such as filamentous actin and myosin. here we present confocal and transmission electron microscopy studies of this process. a sequence in the formation of the contractile ring was shown with rhodamine-phalloidine staining. ultrastructural analysis revealed the presence of fibrilar aggregates in the cytoplasm of dividing trophozoites. among them two filaments of different diameter were identified. these aggrega ... | 2008 | 17870071 |
| amoebiasis: current status in australia. | | 2007 | 17874993 |
| parasitic infections among orang asli (aborigine) in the cameron highlands, malaysia. | in april 2004, an outbreak of acute diarrheal illness occurred among the orang asli (aborigine) in the cameron highlands, pahang state, peninsular malaysia, where rotavirus was later implicated as the cause. in the course of the epidemic investigation, stool samples were collected and examined for infectious agents including parasites. soil transmitted helminthes (sth), namely ascaris lumbricoides (25.7%), trichuris trichiura (31.1%) and hookworm (8.1%), and intestinal protozoa, which included g ... | 2007 | 17877212 |
| forest fragmentation, the decline of an endangered primate, and changes in host-parasite interactions relative to an unfragmented forest. | forest fragmentation may alter host-parasite interactions in ways that contribute to host population declines. we tested this prediction by examining parasite infections and the abundance of infective helminths in 20 forest fragments and in unfragmented forest in kibale national park, uganda. over 4 years, the endangered red colobus (procolobus rufomitratus) declined by 20% in fragments, whereas the black-and-white colobus (colobus guereza) in fragments and populations of both colobines in unfra ... | 2008 | 17879941 |
| entamoeba histolytica mitosomes: organelles in search of a function. | it has been more than eight years since the discovery of mitosomes (mitochondrial remnant organelles) in the intestinal human pathogen entamoeba histolytica. despite detailed knowledge about the biochemistry of this parasite and the completion of the e. histolytica genome sequencing project no physiological function has yet been unequivocally assigned to these organelles. entamoeba mitosomes seem to be the most degenerate of all endosymbiosis-derived organelles studied to date. they do not appea ... | 2008 | 17880942 |
| structural and functional analysis of the entamoeba histolytica ehrabb gene promoter. | the entamoeba histolytica ehrabb gene encodes for a rab gtpase involved in phagocytosis. it is located at a virulence locus where the ehcp112 gene is in the complementary strand at 332 bp of ehrabb start codon, suggesting a finely regulated transcription of both genes. however, the transcription regulation in this parasite is poorly understood. | 2007 | 17883848 |
| caenorhabditis elegans pgp-5 is involved in resistance to bacterial infection and heavy metal and its regulation requires tir-1 and a p38 map kinase cascade. | animals and plants respond to bacterial infections and environmental stresses by inducing overlapping repertoires of defense genes. how the signals associated with infection and abiotic stresses are differentially integrated within a whole organism remains to be fully addressed. we show that the transcription of a caenorhabditis elegans abc transporter, pgp-5 is induced by both bacterial infection and heavy metal stress, but the magnitude and tissue distribution of its expression differs, depend ... | 2007 | 17888400 |
| an epitope-specific pcr test for diagnosis of leishmania donovani infections. | the carboxy-terminal region of recombinant heat shock protein 70 of leishmania donovani has been shown to have an immunodominant epitope. we designed a pcr assay using a new set of primers encompassing the gene sequence from 457bp to 927bp, which amplified a segment of the l. donovani genome in a species-specific manner. the assay was sensitive enough to detect 0.5pg of parasite dna, which increased 10-fold (0.05pg) when an internal probe (583-609bp) was used in the southern blot. the assay was ... | 2008 | 17888473 |
| progress and prospects of gene inactivation in entamoeba histolytica. | over the last few years, numerous methods have been exploited in the attempt to study entamoeba histolytica gene functions. yet several features of e. histolytica, like their variable dna content and complex ploidity have made it difficult to perform classical genetic studies such as homologous recombination. as a result, the methods currently in use target genes at the protein or rna level. this review summarizes the experimental approaches that have been used to date and it provides an overvie ... | 2008 | 17889851 |
| drug targets from human pathogenic amoebas: entamoeba histolytica, acanthamoeba polyphaga and naegleria fowleri. | in this review we present our search for the presence of drug targets in several species of human pathogenic parasites, mainly the amoebas entamoeba histolytica, acanthamoeba polyphaga and naegleria fowleri. we started with an analysis of the concepts of essentiality and validity of the targets and continue with a description of the main characteristics of pathogenicity of these amoebas. we then proceed to evaluate these targets arranged mainly in seven groups corresponding to: a) enzymes which ... | 2007 | 17897063 |
| involvement of beta 2-integrin in ros-mediated neutrophil apoptosis induced by entamoeba histolytica. | cellular adhesion through beta 2-integrin (cd18) is an important step in signal transduction leading to apoptosis of human neutrophils, and nadph oxidase-derived reactive oxygen species (ros) are essential for neutrophil apoptosis induced by entamoeba histolytica. therefore, we investigated the role of beta 2-integrin-mediated signals in ros-dependent neutrophil apoptosis induced by e. histolytica. entamoeba-induced apoptosis was inhibited by pre-incubation of cells with mab to cd18, but not cd2 ... | 2007 | 17900957 |
| attenuated recombinant yersinia as live oral vaccine carrier to protect against amoebiasis. | various attenuated yersinia enterocolitica strains expressing different sections of the entamoeba histolytica surface lectin via the type iii protein secretion system (t3ss) were assessed for their use to orally vaccinate rodents against invasive amoebiasis. the t3ss was found to efficiently express and secrete or translocate subfragments as well as the entire heavy subunit of the lectin. oral vaccination with recombinant yersinia conferred significant protection against amoebic liver abscess fo ... | 2008 | 17900982 |
| effect of vitamin a and zinc supplementation on gastrointestinal parasitic infections among mexican children. | gastrointestinal parasites continue to be an important cause of morbidity and stunting among children in developing countries. we evaluated the effect of vitamin a and zinc supplementation on infections by giardia lamblia, ascaris lumbricoides, and entamoeba histolytica. | 2007 | 17908741 |
| differential native state ruggedness of the two ca2+-binding domains in a ca2+ sensor protein. | characterization of near-native excited states of a protein provides insights into various biological functions such as co-operativity, protein-ligand, and protein-protein interactions. in the present study, we investigated the ruggedness of the native state of ehcabp using nonlinear temperature dependence of backbone amide-proton chemical shifts. ehcabp is a two-domain ef-hand calcium sensor protein consisting of two ef-hands in each domain and binds four ca2+ ions. it has been observed that ap ... | 2008 | 17912755 |
| invasive amebiasis: an update on diagnosis and management. | in its invasive form, the trophozoite is responsible for clinical syndromes, ranging from classical dysentery to extraintestinal disease with emphasis on hepatic amebiasis. abdominal pain, tenderness and diarrhea of watery stool, sometimes with blood, are the predominant symptoms of amebic colitis. besides the microscopic identification of entamoeba histolytica, diagnosis should be based on the detection of specific antigens in the stool or pcr associated with the occult blood in the stool. ameb ... | 2007 | 17914922 |
| [the evaluation of the techniques used for diagnosis of entamoeba histolytica in stool specimens]. | in this study, stool samples of 9378 patients from different clinics, who presented at the laboratory of the department of parasitology of the dokuz eylul university, faculty of medicine with several gastrointestinal complaints from january 2004 to may 2006, were examined. all stool samples were examined with the saline-lugol method and, in suspicious cases, by trichrome staining, cultivation in robinson's medium and/or antigen detection in stool with the entamoeba celisa path kit. forty-one cas ... | 2007 | 17918056 |
| [a retrospective analysis of the results of a seven-year parasitological examination of stools from malatya state hospital]. | the aim of this study was to determine the prevalence of intestinal parasites and to evaluate changes in the annual rates by retrospective screening of the data from our hospital's parasitology laboratory between the period of 2000 and 2006. parasitological examinations were done with native-lugol, cellophane-tape methods and trichrome staining in ambiguous cases, in a total of 67539 samples. the prevalence of the intestinal parasitosis during the years 2000 and 2006 years were found to be 7.2% ... | 2007 | 17918059 |
| autophagy during proliferation and encystation in the protozoan parasite entamoeba invadens. | autophagy is one of the three systems responsible for the degradation of cytosolic proteins and organelles. autophagy has been implicated in the stress response to starvation, antigen cross-presentation, the defense against invading bacteria and viruses, differentiation, and development. saccharomyces cerevisiae atg8 and its mammalian ortholog, lc3, play an essential role in autophagy. the intestinal protozoan parasite entamoeba histolytica and a related reptilian species, entamoeba invadens, po ... | 2008 | 17923513 |
| profiles of a pathogenic entamoeba histolytica-like variant with variations in the nucleotide sequence of the small subunit ribosomal rna isolated from a primate (de brazza's guenon). | a pathogenic entamoeba histolytica-like variant (jsk2004) with genetic variations and a novel isoenzyme pattern was isolated from a de brazza's guenon. a homology of 99.1% was found between the clones of e. histolytica (hm-1:imss) and jsk2004 in the 1,893 nucleotide bases of the small subunit rrna (ssu-rrna) gene. the dna of the pathogenic amoeba species was also extracted from two sterile liver abscesses during the autopsies of an abyssinian colobus and a geoffroy's spider monkey occurring in t ... | 2007 | 17939358 |
| anti-amebic antibody activity in patients, determined with antigens prepared from virulent parasites (indirect hemagglutination assay and enzyme-linked immunosorbent assay). | the serology of amebiasis is affected by low sensitivity and specificity. | 2007 | 17939629 |
| [infectious aetiologies of travelers' diarrhoea]. | traveler's diarrhoea (td) occurs in 20 to 60% of european or north-american travelers in intertropical areas. following return from endemic zone, malaria must always be evocated in front of febrile diarrhoea. many causative infectious agents are involved in td and their frequency may vary according to destination and seasons. the main agents involved in td are escherichia coli pathovars (especially enterotoxigenic and enteroaggregative e. coli) followed by enteroinvasive bacteria (campylobacter ... | 2007 | 17942257 |
| invasive amebiasis and ameboma formation presenting as a rectal mass: an uncommon case of malignant masquerade at a western medical center. | a 54-year-old man presented with rectal pain and bleeding secondary to ulcerated, necrotic rectal and cecal masses that resembled colorectal carcinoma upon colonoscopy. these masses were later determined to be benign amebomas caused by invasive entamoeba histolytica, which regressed completely with medical therapy. in western countries, the occurrence of invasive protozoan infection with formation of amebomas is very rare and can mistakenly masquerade as a neoplasm. not surprisingly, there have ... | 2007 | 17948943 |
| comparative genomics of transcription factors and chromatin proteins in parasitic protists and other eukaryotes. | comparative genomics of parasitic protists and their free-living relatives are profoundly impacting our understanding of the regulatory systems involved in transcription and chromatin dynamics. while some parts of these systems are highly conserved, other parts are rapidly evolving, thereby providing the molecular basis for the variety in the regulatory adaptations of eukaryotes. the gross number of specific transcription factors and chromatin proteins are positively correlated with proteome siz ... | 2008 | 17949725 |
| a mitosome purification protocol based on percoll density gradients and its use in validating the mitosomal nature of entamoeba histolytica mitochondrial hsp70. | mitochondria are indispensable for aerobic respiration, but many microbial eukaryotes have lost this function through reductive evolution. their modified mitochondria are known as hydrogenosomes or mitosomes depending on whether or not they produce molecular hydrogen. the intestinal parasite entamoeba histolytica contains mitosomes whose role in cellular metabolism is unclear. only three proteins have been shown thus far to reside in these organelles: the molecular chaperones hsp10 and hsp60 and ... | 2007 | 17951687 |
| entamoeba moshkovskii infections in sydney, australia. | entamoeba moshkovskii is considered to be a free-living ameba, which is morphologically similar, but biochemically and genetically different, to entamoeba histolytica and entamoeba dispar. however, recent studies have suggested that e. moshkovskii may be a "potential" pathogen, with infections giving rise to diarrhea and other intestinal disorders. microscopy and polymerase chain reaction (pcr) targeting the 18s ribosomal (r) dna was performed on fecal samples collected from patients presenting ... | 2008 | 17957394 |
| patterns of evolution in the unique trna gene arrays of the genus entamoeba. | genome sequencing of the protistan parasite entamoeba histolytica hm-1:imss revealed that almost all the trna genes are organized into tandem arrays that make up over 10% of the genome. the 25 distinct array units contain up to 5 trna genes each and some also encode the 5s rna. between adjacent genes in array units are complex short tandem repeats (strs) resembling microsatellites. to investigate the origins and evolution of this unique gene organization, we have undertaken a genome survey to de ... | 2008 | 17974548 |
| programmed cell death in entamoeba histolytica induced by the aminoglycoside g418. | this study presents morphological and biochemical evidence of programmed cell death (pcd) in entamoeba histolytica induced by exposure of trophozoites to the aminoglycoside antibiotic g418. morphological characteristics of pcd, including cell shrinkage, reduced cellular volume, nuclear condensation, dna fragmentation and vacuolization were observed, with preservation of trophozoite membrane integrity. pcd is orchestrated biochemically by alterations in intracellular ion fluxes. in g418-treated t ... | 2007 | 17975094 |
| in silico identification of the protein disulfide isomerase family from a protozoan parasite. | protein disulfide isomerase (pdi) enzymes are eukaryotic oxidoreductases that catalyze the correct formation of disulfide bonds during protein folding. structurally they are characterized by the presence of functional thioredoxin-like (trx) domains. for the protozoan parasite causative of the human amebiasis (entamoeba histolytica), the correct formation of disulfide bonds is important for an accurate folding of its proteins, including some virulence factors. however, little is known about the e ... | 2008 | 17981509 |
| protozoadb: dynamic visualization and exploration of protozoan genomes. | protozoadb (http://www.biowebdb.org/protozoadb) is being developed to initially host both genomics and post-genomics data from plasmodium falciparum, entamoeba histolytica, trypanosoma brucei, t. cruzi and leishmania major, but will hopefully host other protozoan species as more genomes are sequenced. it is based on the genomics unified schema and offers a modern web-based interface for user-friendly data visualization and exploration. this database is not intended to duplicate other similar eff ... | 2008 | 17981844 |
| toll-like receptor 9-dependent macrophage activation by entamoeba histolytica dna. | activation of the innate immune system by bacterial dna and dna of other invertebrates represents a pathogen recognition mechanism. in this study we investigated macrophage responses to dna from the intestinal protozoan parasite entamoeba histolytica. e. histolytica genomic dna was purified from log-phase trophozoites and tested with the mouse macrophage cell line raw 264.7. raw cells treated with e. histolytica dna demonstrated an increase in levels of tumor necrosis factor alpha (tnf-alpha) mr ... | 2008 | 17984204 |
| seroprevalence of entamoeba histolytica infection in hiv-infected patients in china. | seroprevalence of entamoeba histolytica infection in hiv-infected individuals from shanghai city, anhui province, and henan province, china, was examined by enzyme-linked immunosorbent assay using crude antigen and a recombinant surface antigen, c-igl, of the parasite. in 215 hiv-infected individuals, the positive rates for these antigens were 12.1% and 7.9%, respectively; these rates were significantly higher than the rates of 3.1% and 0.5%, respectively, in 191 patients with gastrointestinal s ... | 2007 | 17984335 |
| amebiasis presenting as acute appendicitis. | amebiasis presenting as acute appendicitis is extremely rare. the case of a 38-year-old hispanic man who presented to the hospital with symptoms and signs suggestive of acute appendicitis is reported. he underwent laparoscopic appendectomy and the pathologic examination of the appendix revealed multiple trophozoites of entamoeba histolytica. the patient was treated postoperatively with metronidazole for amebiasis, and follow-up stool studies showed no sign of residual infection. the patient has ... | 2007 | 17984748 |
| intestinal parasitic infection among children and neonatus admitted to ibn-sina hospital, sirt, libya. | a total of 350 stool samples from 196 males and 154 female children and neonatus admitted in ibn-sina hospital, sirt, were examined from june 2001 to may 2002, to determine the prevalence of intestinal parasites. intestinal parasitic infections were identified in 196 (56%) of children and neonates. no intestinal helminthic parasites were detected but 13 intestinal protozoan parasites were detected. the most prevalent protozoan was entamoeba histolytica /e. dispar (36.57%); blastocystis hominis ( ... | 2007 | 17985574 |
| development of a pharmacodynamic screening model with entamoeba histolytica. | human amoebiasis caused by entamoeba histolytica is widely distributed in the tropics and subtropics, but also occurring in neighbouring parts of the temperate zones. invasive amoebiasis causes dysentery and, by haematogenous spread, also extra-intestinal hepatic, pulmonary or cerebral abscesses, not rarely fatal conditions. the available anti-amoebic drugs have shortcomings regarding tolerability and efficacy. to facilitate the screening of candidate material, an in vitro system has been develo ... | 2007 | 17987365 |
| amebic colitis: new insights into pathogenesis and treatment. | amebiasis, caused by the protozoan parasite entamoeba histolytica, affects more than 50 million people worldwide, with over 100,000 deaths annually. the majority of cases are asymptomatic; however, significant morbidity and mortality are associated with illness in the remaining 10% of cases. recent advances in the understanding of the mechanism of infection by e. histolytica, the role of the innate immune system, and the role of genetic disposition to infection will allow the development of nove ... | 2007 | 17991346 |
| inhibitors of escherichia coli serine acetyltransferase block proliferation of entamoeba histolytica trophozoites. | the protozoan parasite entamoeba histolytica is the etiologic agent of amebiasis, a major global public health problem, particularly in developing countries. there is an effective anti-amoebic drug available, however its long term use produces undesirable side effects. as e. histolytica is a micro-aerophilic organism, it is sensitive to high levels of oxygen and the enzymes that are involved in protecting against oxygen-stress are crucial for its survival. therefore serine acetyltransferase, an ... | 2008 | 17991467 |
| analysis of the protein kinome of entamoeba histolytica. | protein kinases play important roles in almost all major signaling and regulatory pathways of eukaryotic organisms. members in the family of protein kinases make up a substantial fraction of eukaryotic proteome. analysis of the protein kinase repertoire (kinome) would help in the better understanding of the regulatory processes. in this article, we report the identification and analysis of the repertoire of protein kinases in the intracellular parasite entamoeba histolytica. using a combination ... | 2008 | 18004777 |
| epidemiology and aetiology of diarrhoeal diseases in adults engaged in wastewater-fed agriculture and aquaculture in hanoi, vietnam. | to determine the occurrence of and risk factors for diarrhoea in an adult population exposed to wastewater used for agricultural and aquacultural productions. | 2007 | 18005312 |
| destruction of water-borne cysts of entamoeba histolytica by synthetic detergents. | | 1945 | 18016130 |
| hepatic and inferior vena caval thrombosis: vascular complication of amebic liver abscess. | although amebic liver abscess is a common disease, it is a rare cause of venous thrombosis. computed tomography (ct) scan is an ideal tool for diagnosing the various complications associated with liver abscess. we describe a case of amebic liver abscess in a patient who developed a rare vascular complication of hepatic vein and inferior vena cava thrombosis. | 2008 | 18022781 |
| entamoeba histolytica: genetic diversity of african strains based on the polymorphism of the serine-rich protein gene. | the polymorphism of the serine-rich entamoeba histolytica protein (srehp) among isolates obtained from different geographic regions was analyzed by a nested pcr followed by restriction analysis. thirteen different profiles were generated from 23 e. histolytica isolates from cameroon, zimbabwe and south africa while 20 others were generated from 38 e. histolytica pcr positive stool samples from south africa. one of the profiles was common to isolates from cameroon, zimbabwe and south africa and c ... | 2008 | 18028911 |
| regulation of the inflammatory immune response by the cytokine/chemokine network in amoebiasis. | amoebiasis is caused by the protozoa entamoeba histolytica and persists as one of the leading parasitic diseases affecting millions worldwide. this parasite invades the intestinal mucosa, causing amoebic colitis and ulcers. it may also spread to other organs, mainly the liver, causing amoebic liver abscess (ala). current research efforts have focused on the development of specific diagnostic tests and animal models searching for a better understanding of the complex physiopathology of this disea ... | 2007 | 18042174 |
| novel mitochondrion-related organelles in the anaerobic amoeba mastigamoeba balamuthi. | unicellular eukaryotes that lack mitochondria typically contain related organelles such as hydrogenosomes or mitosomes. to characterize the evolutionary diversity of these organelles, we conducted an expressed sequence tag (est) survey on the free-living amoeba mastigamoeba balamuthi, a relative of the human parasite entamoeba histolytica. from 19 182 ests, we identified 21 putative mitochondrial proteins implicated in protein import, amino acid interconversion and carbohydrate metabolism, two c ... | 2007 | 18045382 |
| colitis amebiasis with symptom of occasional dripped anal bleeding. | colitis amebiasis is usually characterized by bloody and mucous diarrhea, abdominal pain and anal discomfort. however, there is unusual manifestation of colitis amebiasis, such as occasional dripped anal bleeding, which sometimes spouted. therefore, we often do not suspect such symptoms for colitis amebiasis. laboratory examination includes complete laboratory test, coagulation and hematologic test, ict tbc and colonoscopy. the pathology anatomy examination reveals positive results of trophozoit ... | 2007 | 18046065 |
| [elisa test for the detection of entamoeba histolytica in stool specimens]. | | 2007 | 18053480 |
| structure and content of the entamoeba histolytica genome. | the intestinal parasite entamoeba histolytica is one of the first protists for which a draft genome sequence has been published. although the genome is still incomplete, it is unlikely that many genes are missing from the list of those already identified. in this chapter we summarise the features of the genome as they are currently understood and provide previously unpublished analyses of many of the genes. | 2007 | 18063096 |
| syntheses and evaluation of 3-(3-bromo phenyl)-5-phenyl-1-(thiazolo [4,5-b] quinoxaline-2-yl)-2-pyrazoline derivatives. | a variety of 3-(3-bromo phenyl)-5-phenyl-1-(thiazolo [4,5-b] quinoxaline-2-yl)-2-pyrazoline were obtained by the refluxing of 1-n-thiocarbamoyl 3,5-diphenyl-2-pyrazoline with 2,3-dichloroquinoxaline. the chemical structures of the compounds were elucidated by uv, ir, (1)h nmr, and (13)c nmr spectroscopy. the purity of the compounds was confirmed by their elemental analysis. the antiamoebic activity of these compounds was evaluated by microdilution method against hmi:imss strain of entamoeba hist ... | 2008 | 18068273 |
| synthesis, spectral studies and antiamoebic activity of new 1-n-substituted thiocarbamoyl-3-phenyl-2-pyrazolines. | thirty new pyrazoline derivatives were synthesized by cyclization of mannich bases with thiosemicarbazides being substituted by different cyclic and aromatic amines. the structures of the compounds were elucidated by elemental analyses, uv, ir, (1)h and (13)c nmr and esi-ms spectral data. the in vitro antiamoebic activity was evaluated against entamoeba histolytica in comparison with metronidazole used as reference substance. out of the 30 compounds screened for antiamoebic activity, 10 (5, 6, 1 ... | 2009 | 18068873 |
| entamoeba histolytica: cyst-like structures in vitro induction. | the cyst of entamoeba histolytica is responsible for amebiasis infection. however, no axenic in vitro system exists that promotes mass encystation for studying this process of this human-infecting parasite. cyst-like structures of e. histolytica obtained in this work were induced using tyi-s-33 media in combination with enterobacterias escherichia coli and enterococcus faecalis conditioned media, high co2 tension and histamine. cyst-like structures showed the same characteristics of a typical e. ... | 2008 | 18083165 |
| participation of the serine-rich entamoeba histolytica protein in amebic phagocytosis of apoptotic host cells. | entamoeba histolytica is an intestinal ameba that causes dysentery and liver abscesses. cytotoxicity and phagocytosis of host cells characterize invasive e. histolytica infection. prior to phagocytosis of host cells, e. histolytica induces apoptotic host cell death, using a mechanism that requires contact via an amebic galactose-specific lectin. however, lectin inhibition only partially blocks phagocytosis of already dead cells, implicating at least one additional receptor in phagocytosis. to id ... | 2008 | 18086807 |
| antimicrobial susceptibility of shigella flexneri and s. dysenteriae isolated from stool specimens of patients with bloody diarrhoea in mwanza, tanzania. | this study was conducted to determine frequency and pattern of antimicrobial susceptibility of shigella species isolated from stool specimens collected from patients presenting with bloody diarrhoea in mwanza city, tanzania. the study was carried out from october 2004 to october 2005 and involved patients attending sekou toure regional hospital and butimba health centre. bacteriological cultures were done at the national institute for medical research laboratory. a total of 489 patients (median ... | 2007 | 18087897 |
| [probable amebic brain abscess in a homosexual man with an entamoeba histolytica liver abscess]. | a 51-year-old japanese-brazilian homosexual man was admitted to a hospital because of fever, headache and right epigastralgia. he had been homosexual for 20 years. an abdominal ct revealed a liver abscess and microscopic examination of the pus of the drainage revealed cystic forms of entamoeba histolytica. oral administration of metronidazole 2,250 mg/day was started for amebic liver abscess. he complained of severe throbbing headache, and magnetic resonance imaging (mri) of the brain showed a b ... | 2007 | 18095503 |
| the use of streptomycin in the cultivation of endameba histolytica from stools. | | 1948 | 18107289 |
| the synergistic action of penicillin and streptomycin on endameba histolytica cultures. | | 1949 | 18110330 |
| studies on the growth requirements of endamoeba histolytica; further observations on the cultivation of e. histolytica and other intestinal protozoa in a clear medium without demonstrable bacterial multiplication; some modifications and simplifications of the medium. | | 1949 | 18113213 |
| the viability of e. histolytica cysts in soil. | | 1949 | 18116846 |
| the effect of acetic acid on the viability of endamoeba histolytica cysts. | | 1949 | 18116847 |
| experimental studies on the role of filth flies in the transmission of endamoeba histolytica. | | 1949 | 18121206 |
| a preliminary note on routine culture methods for endamoeba histolytica. | | 1949 | 18124446 |
| nature of the action of emetine upon entamoeba histolytica. | | 1949 | 18129706 |
| [not available]. | | 1949 | 18132393 |
| the food habits of entamoeba histolytica. | | 1949 | 18139101 |
| susceptibility of the guinea pig to endamoeba histolytica of human origin. | | 1949 | 18141384 |
| routine culture methods in diagnosing endamoeba histolytica. | | 1949 | 18148876 |
| [not available]. | | 1949 | 18149819 |
| everyday and exotic foodborne parasites. | everyday foodborne parasites, which are endemic in canada, include the protozoans entamoeba histolytica, giardia lamblia and cryptosporidium parvum. however, these parasites are most frequently acquired through unfiltered drinking water, homosexual activity or close personal contact such as in daycare centres and occasionally via a food vehicle. it is likely that many foodborne outbreaks from these protozoa go undetected. transmission of helminth infections, such as tapeworms, is rare in canada ... | 2000 | 18159283 |
| a cross-sectional study of intestinal parasitic infections in a rural district of west china. | parasitic infections are widespread in rural areas of west china. the remote and humid environment, traditional ways of life, contaminated potable water and limited health services all contribute to the transmission and persistence of fecal parasites. | 2003 | 18159450 |
| the endoplasmic reticulum chaperone calreticulin is recruited to the uropod during capping of surface receptors in entamoeba histolytica. | calreticulin (crt), an intracellular chaperone protein, is crucial for proper folding and transport of proteins through the endoplasmic reticulum (er). it has recently been identified as a critical regulator of some several different cellular functions such as migration, phagocytosis of apoptotic cells and cytotoxic t lymphocyte- or natural killer cell-mediated lysis. characterization of crt isolated from parasites may thus help to decipher the contribution of this protein in the parasites' biol ... | 2008 | 18160113 |
| two or more enteropathogens are associated with diarrhoea in mexican children. | diarrhoeal diseases constitute a major public health problem, particularly in the developing world, where the rate of mortality and morbidity is very high. the purpose of this study was to conduct a 2 years and 3 months study in order to determine the prevalence of five enteropathogen diarrheogenic agents in mexico city. | 2007 | 18162140 |
| the cys-asn-ser carboxyl-terminal end group is the pharmacophore of the amebic anti-inflammatory monocyte locomotion inhibitory factor (mlif). | the monocyte locomotion inhibitory factor (mlif) is an anti-inflammatory oligopeptide produced by entamoeba histolytica. among its different effects, it inhibits locomotion of human monocytes, hence its original name. previous experimental studies have shown that the anti-inflammatory properties of mlif (met-gln-cys-asn-ser) remained when aminoacid glutamine was substituted by a proline in the second position (pmlif: met-pro-cys-asn-ser). by changing the order of mlif amino acids, the resulting ... | 2008 | 18180049 |
| shouldering a burden. | | 2008 | 18187066 |
| bis-pyrazolines: synthesis, characterization and antiamoebic activity as inhibitors of growth of entamoeba histolytica. | the cyclization of chalcone with n-4 substituted thiosemicarbazides under basic condition led to the formation of new compounds, thiocarbamoyl bis-pyrazoline derivatives. the structure of the compounds were elucidated by uv, ir, (1)h nmr, (13)c nmr and esi-ms spectral data and thermogravimetric analysis, and their purities were confirmed by elemental analyses. the antiamoebic activity of these complexes was evaluated by microdilution method against hm1:imss strain of entamoeba histolytica and th ... | 2009 | 18187238 |
| trichostatin a regulates peroxiredoxin expression and virulence of the parasite entamoeba histolytica. | histone deacetylation is associated with a repressed chromatin state, and histone acetylase and deacetylase activities have been previously described in entamoeba histolytica. to investigate their roles in the control of entamoeba gene expression, the parasite was grown in 50 nm trichostatin a (tsa), an inhibitor of histone deacetylase. tsa enhanced the cytopathic and hemolytic activity of the parasite and its resistance to oxidative stress. we first focused our attention on peroxiredoxin, a pro ... | 2008 | 18191469 |
| evaluation of faecal occult blood test and lactoferrin latex agglutination test in screening hospitalized patients for diagnosing inflammatory and non-inflammatory diarrhoea in dhaka, bangladesh. | inflammatory diarrhoea (id) resulting from shigella, salmonella, campylobacter and entamoeba histolytica requires specific diagnosis for therapy. differentiation between id and non-inflammatory diarrhoea (nid) is often not clinically possible. a faecal occult blood test (fobt) correlates with faecal leucocytes. lactoferrin indicates an inflammatory process as a marker for faecal leucocytes. we evaluated diagnostic values of lactoferrin latex agglutination test (lt) either alone or in combination ... | 2008 | 18196900 |
| kinetic characterization of methionine gamma-lyases from the enteric protozoan parasite entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis. | methionine gamma-lyase (mgl) (ec 4.4.1.11), which is present in certain lineages of bacteria, plants, and protozoa but missing in mammals, catalyzes the single-step degradation of sulfur-containing amino acids (saas) to alpha-keto acids, ammonia, and thiol compounds. in contrast to other organisms possessing mgl, anaerobic parasitic protists, namely entamoeba histolytica and trichomonas vaginalis, harbor a pair of mgl isozymes. the enteric protozoon en. histolytica shows various unique aspects i ... | 2008 | 18199285 |
| intestinal protozoa and helminths among terena indians in the state of mato grosso do sul: high prevalence of blastocystis hominis. | a parasitological survey was carried out among terena indians living in the tereré settlement in the municipality of sidrolândia, state of mato grosso do sul, brazil. single samples of feces from 313 indians were processed by means of the spontaneous sedimentation method. in the population studied, 73.5% were infected with at least one intestinal parasite or commensal. protozoa predominated. blastocystis hominis (40.9%), entamoeba coli (33.2%) and entamoeba histolytica/entamoeba dispar (31.6%) w ... | 2007 | 18200414 |
| entamoeba histolytica phagocytosis of human erythrocytes involves patmk, a member of the transmembrane kinase family. | entamoeba histolytica is the cause of amebic colitis and liver abscess. this parasite induces apoptosis in host cells and utilizes exposed ligands such as phosphatidylserine to ingest the apoptotic corpses and invade deeper into host tissue. the purpose of this work was to identify amebic proteins involved in the recognition and ingestion of dead cells. a member of the transmembrane kinase family, phagosome-associated tmk96 (patmk), was identified in a proteomic screen for early phagosomal prote ... | 2008 | 18208324 |
| intestinal parasitic infections among school children in thailand. | a study was conducted to determine the prevalence of intestinal parasites in children from eight schools located in phuttamonthon district, nakhon prathom province during november 2004 to december 2004. stool samples were collected from 1920 students; age range from 7 to 12 years old, and examined for intestinal parasites by using formalin-ethyl acetate concentration technique. of these subjects, 242 (12.6%) were infected with one or more of 10 intestinal parasitic species. in these infected sub ... | 2007 | 18209713 |
| the cultivation of the small race of endamoeba histolytica with a single species of bacteria. | | 1949 | 18213854 |
| drug effects on drug targets: inhibition of enzymes by neuroleptics, antimycotics, antibiotics and other drugs on human pathogenic amoebas and their anti-proliferative effects. | this paper reviews the inhibition of various enzymes by neuroleptics, anti-mycotics, antibiotics and other drugs on three species of human pathogenic amoebas, mainly entamoeba histolytica, acanthamoeba polyphaga and naegleria fowleri, and their antiproliferative effects. a recent patent registered by philip relates to the combination of an antibacterial formulation and antifungal agent for producing a therapeutically effective quantity of an antimicrobial that is suitable for suppressing or trea ... | 2007 | 18221178 |
| new pd(ii) complexes of the synthesized 1-n-substituted thiosemicarbazones of 3-indole carboxaldehyde: characterization and antiamoebic assessment against e. histolytica. | reaction of 3-indole carboxaldehyde with aminothiocarbonyl hydrazines resulted in the formation of 3-indole carboxaldehyde thiosemicarbazones (tscs) 1-13. the synthesized thiosemicarbazones were used as ligands in the formation of [pd(tsc)cl2] complexes with palladium(ii) metal ion precursor, [pd(dmso)2cl2]. the chemical structures of all the compounds were established by electronic, ir, 1h nmr and 13c nmr spectral data. the structure of the complexes was further established by fabms and dta. it ... | 2008 | 18222017 |
| [evaluation of intestinal parasites in a period of four years in the coprology laboratory of a training hospital]. | parasitological examination of stool samples of 9867 individuals, submitted from both inpatient and outpatient departments to the microbiology department of an 800-bed training hospital between 1.1.2003 and 31.12.2006, revealed that 582 individuals (5.9%) were infected with intestinal parasites. different diagnostic methods were employed in two different periods, and blastocystis hominis, giardia intestinalis and entamoeba histolytica/dispar were found to be the most common parasites, respective ... | 2007 | 18224623 |
| [prevalence of intestinal parasites among workers in food sector in van region]. | stool and cellophane tape specimens were taken for parasitological examination from 739 people who work in food sector and applied to the public health lab of the van health administration for porter examination. parasites were determined at 131 people (17.71%) of 739 worker whom samples were investigated. ninety-five people had one, 30 people had two, 5 people had three and one person had four parasite species. parasites determined in the study were 19.08% helminthes and 80.91% protozoon. in th ... | 2007 | 18224624 |
| morphometric study of the hepatic lesions experimentally induced in hamsters by entamoeba dispar and e. histolytica. | evolution of experimental hepatic lesions produced in hamsters with entamoeba histolytica and e. dispar was evaluated quantitatively and qualitatively through morphometry and immunohistochemistry. animals infected with e. dispar developed hepatic lesions quantitatively and qualitatively similar to those produced by e. histolytica on the first three days of infection. on the 6th and 8th days of infection, e. histolytica produced larger tissue damage than e. dispar. a gradual decrease was observed ... | 2007 | 18225422 |
| characterization of the entamoeba histolytica ornithine decarboxylase-like enzyme. | the polyamines putrescine, spermidine, and spermine are organic cations that are required for cell growth and differentiation. ornithine decarboxylase (odc), the first and rate-limiting enzyme in the polyamine biosynthetic pathway, is a highly regulated enzyme. | 2008 | 18235846 |
| intestinal parasitism, potable water availability and methods of sewage disposal in three communities in benue state, nigeria: a survey. | to assess the level of parasite burden in a village community and the predisposing factors. | 2007 | 18240486 |
| synthesis and antiamoebic activity of metronidazole thiosemicarbazone analogues. | repeated treatment of entamoeba histolytica infection with commonly used antiamoebic drugs results in not only increasing the toxicity potential but also leads to the development of clinical resistance. thus new effective agents with less toxicity against amoebiasis are urgently required. with this view, metronidazole thiosemicarbazone analogues 1-11 were synthesized wherein thioamide moiety was substituted by different cyclic and aromatic amines. these compounds were screened against hm1:imss s ... | 2008 | 18242785 |
| detection of entamoeba histolytica dna from liver abscess aspirate using polymerase chain reaction (pcr): a diagnostic tool for amoebic liver abscess. | amoebic liver abscess is common in bangladesh. it is usually diagnosed by suggestive clinical features, ultrasound findings and positive serology. however, none of these are definitive and the picture overlaps with pyogenic liver abscess. it is critical to differentiate amoebic liver abscess from pyogenic liver abscess as the treatment are different. this study was designed to evaluate the feasibility of using polymerase chain reaction (pcr) for detection of entamoeba histolytica (e.histolytica) ... | 2007 | 18246730 |
| antibody and cytokine responses in dracunculus medinensis patients at distinct states of infection. | dracunculiasis is a promising candidate for eradication, but transmission of dracunculus medinensis and recrudescence of the disease have been observed repeatedly. in the present investigation, the d. medinensis-specific cellular cytokine response profiles and the parasite-specific antibody subclass reactivity were evaluated in dracunculiasis patients at distinct states of infection. analysis of the cellular cytokine response in dracunculiasis patients disclosed a d. medinensis antigen-specific ... | 2008 | 18258273 |
| thermal stabilization of the protozoan entamoeba histolytica alcohol dehydrogenase by a single proline substitution. | analysis of the three-dimensional structures of two closely related thermophilic and hyperthermophilic alcohol dehydrogenases (adhs) from the respective microorganisms entamoeba histolytica (ehadh1) and thermoanaerobacter brockii (tbadh) suggested that a unique, strategically located proline residue (pro275) at the center of the dimerization interface might be crucial for maintaining the thermal stability of tbadh. to assess the contribution of pro275 to the thermal stability of the adhs, we app ... | 2008 | 18260103 |
| bioinformatics and functional analysis of an entamoeba histolytica mannosyltransferase necessary for parasite complement resistance and hepatical infection. | the glycosylphosphatidylinositol (gpi) moiety is one of the ways by which many cell surface proteins, such as gal/galnac lectin and proteophosphoglycans (ppgs) attach to the surface of entamoeba histolytica, the agent of human amoebiasis. it is believed that these gpi-anchored molecules are involved in parasite adhesion to cells, mucus and the extracellular matrix. we identified an e. histolytica homolog of pig-m, which is a mannosyltransferase required for synthesis of gpi. the sequence and str ... | 2008 | 18270556 |
| sampling gene diversity across the supergroup amoebozoa: large est data sets from acanthamoeba castellanii, hartmannella vermiformis, physarum polycephalum, hyperamoeba dachnaya and hyperamoeba sp. | from comparative analysis of est data for five taxa within the eukaryotic supergroup amoebozoa, including two free-living amoebae (acanthamoeba castellanii, hartmannella vermiformis) and three slime molds (physarum polycephalum, hyperamoeba dachnaya and hyperamoeba sp.), we obtained new broad-range perspectives on the evolution and biosynthetic capacity of this assemblage. together with genome sequences for the amoebozoans dictyostelium discoideum and entamoeba histolytica, and including partial ... | 2008 | 18276190 |
| evidence of a continuous endoplasmic reticulum in the protozoan parasite entamoeba histolytica. | entamoeba histolytica, the cause of amebiasis, is believed to have no continuous endoplasmic reticulum (er), with er functions occurring in vesicles. here, using an er-targeted green fluorescent protein fusion protein and fluorescence loss in photobleaching, we have unambiguously demonstrated the presence of a continuous er compartment in living e. histolytica trophozoites. | 2008 | 18281599 |
| increased risk for entamoeba histolytica infection and invasive amebiasis in hiv seropositive men who have sex with men in taiwan. | incidence of entamoeba histolytica infection and clinical manifestations and treatment response of invasive amebiasis (ia) in hiv-infected patients have rarely been investigated before. | 2008 | 18301730 |
| il-6 ko mice develop experimental amoebic liver infection with eosinophilia. | interleukin 6 (il-6) is a multifunctional cytokine that regulates various aspects of the immune response, such as acute phase reaction and hematopoiesis, and is an important signal that coordinates activities of liver cells, macrophages, and lymphocytes. amoebic liver lesions have been studied, usually in hamsters, due to the problem of abscess development in mice. we report here the development of an experimental amoebic liver abscess (ala) model in mice deficient in il-6. axenically grown amoe ... | 2007 | 18314689 |
| calcium-binding protein 1 of entamoeba histolytica transiently associates with phagocytic cups in a calcium-independent manner. | ehcabp1, a calcium-binding protein of the parasite entamoeba histolytica, is known to participate in cellular processes involving actin filaments. this may be due to its direct interaction with actin. in order to understand the kinetics of ehcabp1 in such processes, its movement was studied in living cells expressing gfp-ehcabp1. the results showed that ehcabp1 accumulated at phagocytic cups and pseudopods transiently. the time taken for appearance and disappearance of ehcabp1 was found to be ar ... | 2008 | 18341598 |
| communicable diseases in the immigrant population attended to in a tropical medicine unit: epidemiological aspects and public health issues. | for geographical and historical reasons, spain is receiving an increasing number of immigrants. the aim of this study was to evaluate some epidemiological aspects and the main public health issues of communicable diseases in barcelona's immigrant population. from 2001 to 2004, a population of immigrants from tropical, subtropical regions and eastern europe was attended to in our centre. each patient was offered a complete screening for tropical and common diseases. the prevalence and demographic ... | 2008 | 18342267 |