| transcriptional and secretory responses of entamoeba histolytica to mucins, epithelial cells and bacteria. | invasive intestinal amebiasis, caused by entamoeba histolytica, is initiated with attachment of trophozoites to the colonic mucous layer, mucous disruption and/or depletion, and adherence to and cytolysis of host epithelial and inflammatory cells. a current working model of intestinal amebiasis suggests that the microenvironment of the host intestine, particularly intestinal mucins and the bacterial biofilm, may influence the behavior of pathogenic amebae. the invasive phenotype is dependent on ... | 2007 | 17362964 |
| [advances in research on virulence factors of entamoeba histolytica]. | the parasitic protozoan entamoeba histolytica is an important pathogen responsible for intestinal and extraintestinal amebiasis in human, claiming up to 100 000 victims every year. this parasite is characterized by its high potential for invading and destroying human tissues. invasion past the mucous barrier of the colonic epithelium leads to the development of colitis and amebic dysentery. dissemination of the trophozoite through blood stream permits the invasion of other tissues, resulting in ... | 2006 | 17366980 |
| immunohistochemical characterization of human fulminant amoebic colitis. | in cases of fulminant amoebic colitis we have determined the interactions between entamoeba histolytica trophozoites and immune cells in order to better understand the pathophysiology of amoebic colitis. eleven specimens of amoebic colitis and five specimens of colon without amoebic lesions were studied. trophozoites and immune cells were located by topographic stains, histochemistry and immunohistochemistry. trophozoites were seen in both damaged and undamaged areas of the colonic mucosa. speci ... | 2007 | 17371457 |
| [pathogenic entamoeba histolytica--a rare incidence in persons microscopically positive for cysts in faeces]. | in the czech republic, 40 to 50 cases of entamoeba histolytica/dispar infections are reported annually to the national reference laboratory for diagnostics of intestinal parasitoses. however, the actual number of patients with entamoeba histolytica infection is unknown as diagnosis relies on cyst detection in faecal samples by microscopy, the method which cannot differentiate between pathogenic e. histolytica and nonpathogenic e. dispar. the aim of this study was (1) to evaluate the proportions ... | 2007 | 17373108 |
| is this recently characterized gastrointestinal pathogen responsible for rising rates of inflammatory bowel disease (ibd) and ibd associated autism in europe and the united states in the 1990s? | in 2006, a pathogenic variant of the common intestinal organism blastocystis was discovered in patients who were experiencing chronic gastrointestinal symptoms. most species of blastocystis inhabit humans with no symptoms. the discovery of a pathogenic variant of blastocystis is significant, because blastocystis is related to entamoeba, a similar organism with pathogenic variants that kill over 100,000 people each year. recent research has shown that blastocystis infections may be undetectable u ... | 2007 | 17382484 |
| outcome of patients with arthritis and parvovirus b19 dna in synovial membranes. | to investigate the follow-up of the 17 patients during the period of 1995-2001 of the outpatient clinic for rheumatology at the university hospital of zurich with arthritis and the presence of parvovirus b19 dna demonstrated by pcr in synovial biopsies. seventeen patients of 163 with arthritis, which were routinely examined by needle arthroscopy during 1995-2001 with a positive parvovirus b19 dna by pcr of synovial biopsy were reevaluated. investigations included medical history, clinical examin ... | 2007 | 17401566 |
| waterborne transmission of protozoan parasites: a worldwide review of outbreaks and lessons learnt. | at least 325 water-associated outbreaks of parasitic protozoan disease have been reported. north american and european outbreaks accounted for 93% of all reports and nearly two-thirds of outbreaks occurred in north america. over 30% of all outbreaks were documented from europe, with the uk accounting for 24% of outbreaks, worldwide. giardia duodenalis and cryptosporidium parvum account for the majority of outbreaks (132; 40.6% and 165; 50.8%, respectively), entamoeba histolytica and cyclospora c ... | 2007 | 17402277 |
| an entamoeba sp. strain isolated from rhesus monkey is virulent but genetically different from entamoeba histolytica. | an entamoeba sp. strain, p19-061405, was isolated from a rhesus monkey in nepal and characterized genetically. the strain was initially identified as entamoeba histolytica using pcr amplification of peroxiredoxin genes. however, sequence analysis of the 18s rrna gene showed a 0.8% difference when compared to the reference e. histolytica hm-1:imss human strain. differences were also observed in the 5.8s rrna gene and the internal transcribed spacer (its) regions 1 and 2, and analysis of the serin ... | 2007 | 17403547 |
| dolichol-linked oligosaccharide selection by the oligosaccharyltransferase in protist and fungal organisms. | the dolichol-linked oligosaccharide glc3man9glcnac2-pp-dol is the in vivo donor substrate synthesized by most eukaryotes for asparagine-linked glycosylation. however, many protist organisms assemble dolichol-linked oligosaccharides that lack glucose residues. we have compared donor substrate utilization by the oligosaccharyltransferase (ost) from trypanosoma cruzi, entamoeba histolytica, trichomonas vaginalis, cryptococcus neoformans, and saccharomyces cerevisiae using structurally homogeneous d ... | 2007 | 17403929 |
| how to diagnose and manage common parasitic pneumonias. | parasitic pneumonia is being increasingly reported from many parts of the world due to globalization and travel across the continents. this review outlines the recent developments in the diagnosis and management of parasitic pneumonias. | 2007 | 17414130 |
| transcriptional upregulation of genes related to virulence activation in entamoeba histolytica. | to understand the molecular basis of virulence variability in entamoeba histolytica, this study presents results about differential gene expression induced by e. histolytica trophozoites in liver of hamsters in order to produce experimental amebic liver abscess (ala) and consequently reactivate its virulence. | 2007 | 17416282 |
| [diagnosis and treatment of acute amebian liver abscess with laparoscopic method]. | laparoscopic surgical treatment was performed at 55 patients with acute amebian liver abscess. the diagnosis of acute amebian liver abscess was verified with clinical, serological, echoscopic, x-ray and ct methods. the laparoscopic method permits to sanify the abscess cavity of any localization. all the 55 patients have been discharged in good health. laparoscopic method is maximally rational, associated with minimal surgical trauma, permits to decrease hospital stay and period of postoperative ... | 2006 | 17419485 |
| multiplex real-time pcr assay for detection of entamoeba histolytica, giardia intestinalis, and cryptosporidium spp. | entamoeba histolytica, giardia intestinalis, and cryptosporidium spp. are not only three of the most important and common diarrhea-causing parasitic protozoa, but they often have similar clinical presentations. microscopic diagnosis of these parasites is neither sensitive nor specific. recently, more specific and sensitive alternative molecular methods (polymerase chain reaction [pcr] and antigen detection tests) have been introduced for all three of these parasitic infections. the use of these ... | 2007 | 17426176 |
| antisense expression of a gene encoding a calcium-binding protein in transgenic tobacco leads to altered morphology and enhanced chlorophyll. | entamoeba histolytica contains a novel calcium-binding protein like calmodulin,which was discovered earlier,and we have reported the presence of its homologue(s)and a dependent protein kinase in plants.to understand the functions of these in plants,a cdna encoding a calcium-binding protein isolated from entamoeba histolytica (ehcabp)was cloned into vector pbi121 in antisense orientation and transgenic tobacco plants were raised.these plants showed variation in several phenotypic characters,of wh ... | 2007 | 17435317 |
| amoebiasis: current status in australia. | entamoeba histolytica is one of the most common parasitic infections worldwide, infecting about 50 million people and resulting in 40,000-100,000 deaths a year. in australia, people at risk of infection include immigrants, travellers returning from countries of high endemicity, indigenous people, and men who have sex with men. clinical manifestations range from asymptomatic carriage to invasive disease. amoebic colitis and amoebic liver abscess are the most common invasive manifestations observe ... | 2007 | 17437396 |
| identification of exocytotic membrane proteins, syntaxin-1 and snap-25, in entamoeba histolytica from hamster liver. | entamoeba histolytica is a protozoan parasite causing dysentery and in some cases liver abscesses. these effects have been attributed to cytolytic substances released by exocytosis. in this study, the presence of the proteins syntaxin-1 and snap-25, which are assumed to be involved in exocytosis, were examined by immunohistochemistry, immunoelectron microscopy and western blot analysis. syntaxin-1 and snap-25 were expressed in the vesicular, vacuolar and plasma membranes of e. histolytica tropho ... | 2007 | 17437528 |
| rab11b small gtpase regulates secretion of cysteine proteases in the enteric protozoan parasite entamoeba histolytica. | vesicular trafficking plays a pivotal role in the virulence of the enteric protozoan parasite entamoeba histolytica. in the present study, we showed that one isotype of the small gtpase rab11, ehrab11b, plays a central role in the secretion of a major virulence factor, cysteine proteases. ehrab11b did not colocalize with markers for the endoplasmic reticulum, early endosomes and lysosomes, but was partially associated with non-acidified vesicles in the endocytic pathway, likely recycling endosom ... | 2007 | 17441984 |
| synthesis, characterization and antiamoebic activity of new indole-3-carboxaldehyde thiosemicarbazones and their pd(ii) complexes. | in continuation of our research on thiosemicarbazones and their metal complexes as antiamoebic agents, a new series of indole-3-carboxaldehyde thiosemicarbazones (tsc) 1-7 were prepared by condensing indole-3-carboxaldehyde with cycloalkylaminothiocarbonyl hydrazines. their palladium(ii) complexes of the [pd(tsc)cl2] type, were synthesized upon coordination with [pd(dmso)2cl2]. the chemical structures of all the compounds were established by elemental analyses, electronic, ir, (1)h nmr and (13)c ... | 2007 | 17442460 |
| effect of intensive handwashing in the prevention of diarrhoeal illness among patients with aids: a randomized controlled study. | patients with aids frequently develop diarrhoeal illness. in this randomized, controlled study, 260 patients were screened for those who had not had diarrhoea in the preceding 3 months and who had received a stable highly active antiretroviral therapy regimen for at least 6 weeks prior to the study enrollment. a total of 148 patients met the inclusion criteria and were enrolled: 75 patients were randomly assigned to an intensive handwashing intervention (i.e. handwashing after defecation, after ... | 2007 | 17446290 |
| cryptosporidiosis among immunocompetent patients with gastroenteritis in iran: a comparison with other enteropathogenic parasites. | cryptosporidium is a protozoan parasite that reproduces within the epithelial cells of several organs of vertebrate hosts. the manifestation of the disease is either self-limiting acute diarrhea in immunocompetent patients, or fatal chronic diarrhea in immunocompromised patients. common clinical symptoms include watery diarrhea, abdominal pain, and weight loss. | 2007 | 17446964 |
| thioredoxin-linked metabolism in entamoeba histolytica. | entamoeba histolytica, an intestinal protozoan that is the causative agent of amoebiasis, is exposed to elevated amounts of highly toxic reactive oxygen species during tissue invasion. in this work, we report the molecular cloning, from e. histolytica genomic dna, of the genes ehtrxr and ehtrx41, respectively coding for thioredoxin reductase (ehtrxr) and thioredoxin (ehtrx41). the genes were expressed in escherichia coli cells, and the corresponding recombinant proteins were purified and charact ... | 2007 | 17448896 |
| a novel amoeba proteus 120 kda actin-binding protein with only 1 filamin repeat and a coiled-coil region. | a novel 120 kda actin-binding protein (apabp-f1) was found in amoeba proteus. it was distributed throughout the cytoplasm, mainly in the subplasma membrane and perinuclear-nuclear areas, enriched in actin. the full-length cdna of apabp consisted of 2672 nucleotides with an open reading frame of 878 amino acids, giving a ~95 kda protein with a theoretical pi value of 5.11. it had a novel domain organization pattern: the n terminus (residues 1-104) contained 1 calponin-homology (ch) domain, follow ... | 2007 | 17464342 |
| [hepatic amebiasis]. | | 2006 | 17465190 |
| functional characterization of spliceosomal introns and identification of u2, u4, and u5 snrnas in the deep-branching eukaryote entamoeba histolytica. | pre-mrna splicing is essential to ensure accurate expression of many genes in eukaryotic organisms. in entamoeba histolytica, a deep-branching eukaryote, approximately 30% of the annotated genes are predicted to contain introns; however, the accuracy of these predictions has not been tested. in this study, we mined an expressed sequence tag (est) library representing 7% of amoebic genes and found evidence supporting splicing of 60% of the testable intron predictions, the majority of which contai ... | 2007 | 17468393 |
| [is blastocystis hominis an opportunist agent?]. | despite its high prevalence throughout the world, major issues about blastocystis hominis remain unresolved, including fundamental areas such as taxonomy and pathogenicity. sequences of the ssurrna gene place blastocystis in the stramenophiles. analysis of the elongation factor 1-alpha gene, however, indicates similarity to entamoeba histolytica. there is considerable morphological variability and karyotype diversity, and it appears that more than one species is present in humans and animals. in ... | 2007 | 17471409 |
| [evaluation of parasitological laboratory results of a group of people older than 15 years of age in kutahya]. | this research has been based on microscopic examination of samples of stool specimens taken from 675 persons older than 15 years of age. the examinations were conducted in the parasitological laboratory of the dumlupinar university hospital. laboratory examinations of stools were done with native and formalin-ethyl acetate sedimentation methods. different intestinal parasites were found in 52 (7.7%) of the stool specimens. at least one parasite species was found in 3.11% of women and 4.59% of me ... | 2007 | 17471410 |
| effectiveness of dried carica papaya seeds against human intestinal parasitosis: a pilot study. | the tropical fruit carica papaya and its seeds have proven antihelminthic and anti-amoebic activities. to determine the effectiveness of air-dried c. papaya seeds on human intestinal parasitosis, 60 asymptomatic nigerian children with stool microscopic evidence of intestinal parasites received immediate doses (20 ml) of either an elixir composed with air-dried c. papaya seeds and honey (cph) or honey alone (placebo) in two randomized treatment groups. repeat stool microscopic examinations were c ... | 2007 | 17472487 |
| antiprotozoal activity of senna racemosa. | methanol extracts of leaves, roots and bark of senna racemosa (mill.) h.s. irwin & barneby (syn. cassia racemosa mill.) were tested for antiprotozooal activity against giardia intestinalis and entamoeba histolytica. all of the tested extracts showed good activity against both protozoa species. extracts from stem bark and leaves were most active, with an ic(50) of 2.10 microg/ml for giardia intestinalis and 3.87 microg/ml for entamoeba histolytica. of the previously isolated compounds from senna ... | 2007 | 17481835 |
| [pathogenesis and virulence factors associated with intestinal protozoan parasites]. | | 2007 | 17491420 |
| static fields: biological effects and mechanisms relevant to exposure limits. | recently, the international emf project of the world health organization (who) published an environmental health criteria monograph on static electric and magnetic fields. in the present paper a short overview is given of the biological and health effects discussed in this document. the main conclusions are that no acute effects other than transient phenomena such as vertigo and nausea have been observed with exposure to static magnetic flux densities up to 8 t. there are no reports of long term ... | 2007 | 17495659 |
| calpain-dependent calpastatin cleavage regulates caspase-3 activation during apoptosis of jurkat t cells induced by entamoeba histolytica. | in this study, we investigated whether there is a signalling interaction between calpain and caspase-3 during apoptosis in jurkat t cells by entamoeba histolytica. when jurkat cells were co-incubated with e. histolytica, phosphatidylserine externalisation and dna fragmentation markedly increased compared with results for cells incubated with medium alone. in addition, e. histolytica strongly induced cleavage of caspases-3, -6, -7 and poly(adp-ribose) polymerase. a rise in intracellular calcium l ... | 2007 | 17498717 |
| etiologic agents of diarrhea in solid organ recipients. | after transplantation, diarrhea may be caused by infectious agents, drug-specific effects, metabolic conditions, or mechanical complications of surgery. determining the cause helps to determine whether to initiate antimicrobial therapy and the duration of treatment. in this study we aimed to determine the causes of diarrhea in kidney or liver recipients. fifty-two diarrhea episodes among 43 solid organ recipients were evaluated. the cause of diarrhea was detected in 43 patients (82.6%). infectio ... | 2007 | 17511817 |
| detection of excretory entamoeba histolytica dna in the urine, and detection of e. histolytica dna and lectin antigen in the liver abscess pus for the diagnosis of amoebic liver abscess. | amoebic liver abscess (ala) and pyogenic liver abscesses (pla) appear identical by ultrasound and other imaging techniques. collection of blood or liver abscess pus for diagnosis of liver abscesses is an invasive procedure, and the procedure requires technical expertise and disposable syringes. collection of urine is a noninvasive procedure. therefore, there has been much interest shown towards the use of urine as an alternative clinical specimen for the diagnosis of some parasitic infections. h ... | 2007 | 17511859 |
| novel pd(ii) complexes of 1-n-substituted 3-phenyl-2-pyrazoline derivatives and evaluation of antiamoebic activity. | cyclization of mannich base with n(4)-substituted thiosemicarbazides by different aliphatic, aromatic and cyclic amines afforded a series of new 1-n-substituted cyclised pyrazoline analogues of thiosemicarbazones (pyz-tsc) 1-10. reaction of [pd(dmso)(2)cl(2)] with pyrazoline derivatives led to new palladium(ii) complexes [pd(pyz-tsc)cl(2)] 1a-10a. the structures of all the compounds were characterized by spectroscopic methods. it was concluded that the pyrazoline thiosemicarbazone derivatives ha ... | 2008 | 17513021 |
| use of recombinant entamoeba histolytica cysteine proteinase 1 to identify a potent inhibitor of amebic invasion in a human colonic model. | cysteine proteinases are key virulence factors of the protozoan parasite entamoeba histolytica. we have shown that cysteine proteinases play a central role in tissue invasion and disruption of host defenses by digesting components of the extracellular matrix, immunoglobulins, complement, and cytokines. analysis of the e. histolytica genome project has revealed more than 40 genes encoding cysteine proteinases. we have focused on e. histolytica cysteine proteinase 1 (ehcp1) because it is one of tw ... | 2007 | 17513563 |
| eradication of amebiasis in a large institution for adults with mental retardation in taiwan. | investigation of a program to eradicate amebiasis using consecutive intensive mass screenings followed by medication in a large institute for adults with mental retardation in taiwan. | 2007 | 17520540 |
| a novel nested multiplex polymerase chain reaction (pcr) assay for differential detection of entamoeba histolytica, e. moshkovskii and e. dispar dna in stool samples. | e. histolytica, a pathogenic amoeba, is indistinguishable in its cyst and trophozoite stages from those of non-pathogenic e. moshkovskii and e. dispar by light microscopy. we have developed a nested multiplex pcr targeting a 16s-like rrna gene for differential detection of all the three morphologically similar forms of e. histolytica, e. moshkovskii and e. dispar simultaneously in stool samples. | 2007 | 17524135 |
| adherence-inhibitory intestinal immunoglobulin a antibody response in baboons elicited by use of a synthetic intranasal lectin-based amebiasis subunit vaccine. | we designed an amebiasis subunit vaccine that is constructed by using four peptide epitopes of the galactose-inhibitable lectin heavy subunit that were recognized by intestinal secretory immunoglobulin a (iga) antibodies from immune human subjects. these epitopes are contained in the region encompassing amino acids 758 to 1134 of the lectin heavy subunit, designated lc3. baboons (papio anubis) are natural hosts for entamoeba histolytica; naturally infected baboons raised in captivity possess ser ... | 2007 | 17526742 |
| characterization of entamoeba histolytica alpha-actinin2. | we have cloned and characterized a second alpha-actinin isoform in entamoeba histolytica. this protein, alpha-actinin2, has a n-terminal actin-binding domain, a c-terminal calcium-binding domain and an intervening rod domain containing two spectrin repeats. the protein binds and cross-links actin filaments in a calcium-dependent manner. therefore alpha-actinin2 is a genuine alpha-actinin except for the shorter rod domain compared to the rod domain of isoforms of higher organisms. a alpha-actinin ... | 2007 | 17537529 |
| the potential usefulness of the modified kato thick smear technique in the detection of intestinal sarcocystosis during field surveys. | a total of 479 stool specimens were collected from rural communities of ubon ratchathani province, thailand and examined by two techniques: the modified kato thick smear and the direct smear. the prevalence of opisthorchis viverrini (14.8%), hookworm (10.2%), sarcocystis spp (4.6%), taenia spp (2.9%), strongyloides stercoralis (2.1%), giardia lamblia (1.2%), echinostoma spp (0.6%), ascaris lumbricoides (0.4%), entamoeba histolytica (0.2%), chilomastix mesnili (0.2%) and endolimax nana (0.2%) wer ... | 2007 | 17539271 |
| amebic liver abscess in hiv-infected patients, republic of korea. | | 2007 | 17552123 |
| replacement of the essential dictyostelium arp2 gene by its entamoeba homologue using parasexual genetics. | cell motility is an essential feature of the pathogenesis and morbidity of amoebiasis caused by entamoeba histolytica. as motility depends on cytoskeletal organisation and regulation, a study of the molecular components involved is key to a better understanding of amoebic pathogenesis. however, little is known about the physiological roles, interactions and regulation of the proteins of the entamoeba cytoskeleton. | 2007 | 17553170 |
| crystallization and preliminary crystallographic analysis of cysteine synthase from entamoeba histolytica. | entamoeba histolytica, the causative agent of human amoebiasis, is essentially anaerobic, requiring a small amount of oxygen for growth. it cannot tolerate the higher concentration of oxygen present in human tissues or blood. however, during tissue invasion it is exposed to a higher level of oxygen, leading to oxygen stress. cysteine, which is a vital thiol in e. histolytica, plays an essential role in its oxygen-defence mechanisms. the major route of cysteine biosynthesis in this parasite is th ... | 2007 | 17554175 |
| crystal structure of calcium binding protein-1 from entamoeba histolytica: a novel arrangement of ef hand motifs. | calcium plays a pivotal role in the pathogenesis of amoebiasis, a major disease caused by entamoeba histolytica. several ef-hand containing calcium-binding proteins (cabps) have been identified from e. histolytica. even though these proteins have very high sequence similarity, they bind to different target proteins in a ca2+ dependent manner, leading to different functional pathways (yadava et al., mol biochem parasito 1997;84:69-82; chakrabarty et al., j biol chem 2004;279:12898-12908) the crys ... | 2007 | 17554780 |
| the entamoeba histolytica genome: primary structure and expression of proteolytic enzymes. | a number of studies have shown that peptidases and in particular cysteine peptidases constitute major pathogenicity factors in entamoeba histolytica. recent studies have suggested that a considerable number of genes coding for proteolytic enzymes are present within the e. histolytica genome and questions remain about the mode of expression of the various molecules. | 2007 | 17567921 |
| prevalence of intestinal protozoa in an aborigine community in pahang, malaysia. | the objective was to estimate the prevalence of intestinal protozoa among the aborigines and to determine the problems regarding the infection. the study was carried out in january 2006 in pos senderut, pahang, malaysia. samples of faeces were collected from children and adults and these were fixed in pva and trichrome staining was carried out. from the 130 individuals studied, 94 (72.3%) were positive with at least one intestinal protozoa. nine intestinal protozoa namely blastocystis hominis, g ... | 2007 | 17568378 |
| morbidity due to schistosoma mansoni--entamoeba histolytica coinfection in hamsters (mesocricetus auratus). | data on schistosoma mansoni-entamoeba histolytica coinfection are scarce in the literature. in the present study, hamsters that had been infected for 70 days with schistosoma mansoni (le strain) were inoculated via the portal vein with two strains of trophozoites of entamoeba histolytica: icb-egg (highly virulent) and icb-rps (non-virulent). the most evident result of coinfection was increased morbidity and mortality, in comparison with either of the infections alone. histologically, there were ... | 2007 | 17568883 |
| [urticaria and parasites: case report and general view over the most common pathogens of chronic urticaria]. | chronic urticaria is a disease with numerous aetiologies. in case of blood hypereosinophilia a parasitosis is probable an should be mentioned. infections with echinococcus and helminths can cause ige mediated release of histamine. different cytokines are involved and lead to chronic urticaria as well. there are only a few case repoorts about chronic urticaria caused by amoebiasis. the transmission of amoebas is faeco oral and the pathogen can be detected with stool examinations or with testing o ... | 2007 | 17569439 |
| entamoeba histolytica: ouabain-insensitive na(+)-atpase activity. | our aim was to determine the presence of sodium pumps in entamoeba histolytica. it is shown through the measurement of ouabain-sensitive atpase activity and immunoblotting that e. histolytica does not express (na(+)+k(+))atpase. on the other hand, we observed a na(+)-atpase with the following characteristics: (1) stimulated by na(+) or k(+), but these effects are not addictive; (2) the apparent affinity is similar for na(+) and k(+) (k(0.5) = 13.3 +/- 3.7 and 15.4 +/- 3.1mm, respectively), as we ... | 2007 | 17574551 |
| the role of the secretory immune response in the infection by entamoeba histolytica. | intestinal infection with the protozoan parasite entamoeba histolytica elicits a local immune response with rising of specific secretory iga (siga) antibodies detectable in several compartments associated to mucosa. anti-amoebic siga antibodies have been reported in faeces, saliva, bile and breast milk from dysenteric patients and research trying to elucidate their role in protection has recently intensified. iga antibodies inhibit the in vitro adherence of e. histolytica trophozoites to epithel ... | 2007 | 17576362 |
| [the process of n-glycosylation of proteins in entamoeba histolytica]. | | 2006 | 17578075 |
| effect of solar disinfection on viability of intestinal protozoa in drinking water. | the effect of solar disinfection on the viability of intestinal protozoa giardia lamblia, microsporidia sp., cryptosporidium parvum, cyclospora cyatenensis and entamoeba histolytica in drinking water was studied as compared to chlorine disinfection. the protozoa were collected from stool samples, to infect to the distilled water. chlorinated water samples were prepared at concentration of 4 ppm, and the parasites were incubated overnight at room temperature with the treated water. sun treatment ... | 2007 | 17580569 |
| detection of entamoeba histolytica/dispar in stool specimens by using enzyme-linked immunosorbent assay in the population of jeddah city, saudi arabia. | this study determined the prevalence of intestinal parasites, particularly pathogenic entamoeba sp. (e. histolytica), in patients attending three hospitals in jeddah city, king abdulaziz university hospital, king abdulaziz hospital and king fahad hospital for gastro-intestinal troubles. 186 stool specimens were examined microscopically for parasites and by elisa kit (e. histolytica ii) for true e. histolytica. 83 samples (44.6%) were positive by microscopy for at least one parasite. of which, 23 ... | 2007 | 17580574 |
| prevalence of intestinal parasites among primary schoolchildren in derna district, libya. | the prevalence of intestinal parasites and some hygienic factors was evaluated in primary schoolchildren in derna district. a total of 1039 stool specimens were examined by direct smear and formaline-ether concentration methods. the results showed that 31% of the children were infected with at least one or two parasites. these parasites were giardia lamblia (12.7%), blastocystis hominis (6.7%), entamoeba histolytica/ entamoeba dispar (6.6%), entamoeba coli (3.2%), e. hartmanni (1.0%), enterobius ... | 2007 | 17580578 |
| detection and differentiation of entamoeba histolytica and entamoeba dispar isolates in clinical samples by pcr. | a total of 140 out of 180 outpatients attended misr university for science and technology hospital complained of abdominal pain, diarrhoea and/or dysentery. stool examination showed 47 (33.6%) had entamoeba sp., 36 (25.7%) had cysts and 11 (7.9%) had trophozoites. of 40 asymptomatic ones, 4 (10%) had cysts. a total of 51 positive stool samples for entamoeba sp. (40 cysts & 11 trophozoites) were tested by ne-sted polymerase chain reaction (n-pcr) and restriction enzyme digestion (red) to clarify ... | 2007 | 17580582 |
| entamoeba histolytica: adp-ribosylation of secreted glyceraldehyde-3-phosphate dehydrogenase. | in addition to its classic glycolytic role, glyceraldehyde-3-phosphate dehydrogenase (gapdh) has been implicated in many activities unrelated to glycolysis, such as membrane fusion, binding to host proteins and signal transduction. gapdh can be the target of several modifications that allow incorporation to membranes and possible regulation of its activity; among these modifications is mono-adp-ribosylation. this post-translational modification is important for the regulation of many cellular pr ... | 2007 | 17586498 |
| effect of entamoeba histolytica toxins on isolated human polymorphonuclear leukocytes' phagocytic function. | this study was undertaken to investigate the effect of entamoeba histolytica toxin (ehp/t) obtained from hm1:imss strain on human polymorphonuclear leukocytes (pmns) phagocytic function. a less virulent strain, i.e., nih:200 was also used in this study for comparison. the results revealed that the toxin obtained from the strain hm1:imss inhibited the phagocytic activity of pmns as measured by yeast uptake or chemiluminescence response while the toxin of strain nih:200 did not show any significan ... | 1992 | 17586996 |
| prevalence of intestinal parasites among food handlers in al-medinah. | fecal specimens from 13.216 food handlers (male) adults, representing 21 different nationalities, were analyzed for the presence of intestinal parasites at king abdul azeez hospital, al-medinah. approximately 14% of these individuals harbored potentially pathogenic parasites, the most common organism being giardia lamblia (33%), followed by entamoeba histolytica (23%), trichuris trichiura (12.3%), ascaris lumbricoides (11.8%) and others. the highest incidence of parasite load was found in sri la ... | 1992 | 17589131 |
| prevalence of pathogenic intestinal parasites among preschool children in al-medina district, saudi arabia. | the prevalence of pathogenic intestinal parasites among preschool children in al-medina district, saudi arabia, was determined through a randomized multistage sampling of 8000 preschool children. duplicate specimens from each child were examined using a simple sedimentation technique. the overall prevalence of intestinal parasites among children screened was 18.4%. the most common parasite isolated was giardia lamblia (14.5%). this was followed by entamoeba histolytica (2.7%), enterobius vermicu ... | 1993 | 17590673 |
| genome re-duplication and irregular segregation occur during the cell cycle of entamoeba histolytica. | heterogeneity of genome content is commonly observed in axenic cultures of entamoeba histolytica. cells with multiple nuclei and nuclei with heterogenous genome contents suggest that regulatory mechanisms that ensure alternation of dna synthesis and mitosis are absent in this organism. therefore, several endo-reduplicative cycles may occur without mitosis. the data also shows that unlike other endo-reduplicating organisms, e.histolytica does not undergo a precise number of endo-reduplicative cyc ... | 2007 | 17592766 |
| [nitric oxide participation during amoebic liver abscess development]. | nitric oxide participates in both physiological and pathophysiological functions, and it plays an important role in the mammalian immune system in killing or inhibiting the growth of many pathogens, including parasites, viruses and bacteria. entamoeba histolytica is a protozoan parasite that causes amoebiasis, which is characterized by intestinal damage and amoebic liver abscess development. the development of amoebic liver abscess in hamsters is similar to that in humans, whereas mice are resis ... | 2007 | 17593605 |
| [evaluation of parasitology laboratory results of a group of people older than 15 years of age in kutahya]. | this research has been based on microscopic examination of stool specimens taken from 675 persons older than 15 years of age. the examinations were conducted in parasitology laboratory of the dumlupinar university hospital. laboratory examinations of stools were done with native and formalin-ethyl acetate sedimentation methods. different intestinal parasites were found in 52 (7.7%) of the stool specimens. at least one parasite species was found in 3.11% of women and 4.59% of men. the prevalence ... | 2007 | 17594654 |
| [investigation of intestinal parasites in food workers in hospitals in aydin, turkey]. | food workers are an important risk group for intestinal parasite contamination and dissemination. in the present study food workers, working in food preparation and distribution in the adnan menderes university hospital, aydin state hospital and 82. yil state hospital, were screened for the presence of intestinal parasites. out of 58 food workers 22 were females and 36 were males, and the age of workers ranged from 20 to 56. all workers included in the study answered a questionnaire concerned wi ... | 2007 | 17594656 |
| [distribution of intestinal parasites detected in camlihemşin healthcare center during the period from july 2005 to january 2007]. | in the present study, 142 persons admitted to the camlihemşin healthcare center between july 2005 and january 2007, carriage of intestinal parasites was investigated in 93 male and 49 female patients. all of the stool samples were examined by using native-lugol technique. cellophane tape method was applied to the 0-19 age group. parasite cysts and/or eggs were found in total of 17,6 % of the patients. of the patients carrying parasites, 64% (16/25) was female, and 36% (9/25) was male. giardia in ... | 2007 | 17594658 |
| intestinal parasitism and vibrio cholerae infection among diarrhoeal patients in kolkata, india. | in this study, we have analysed the epidemiological significance of the concurrent infections caused by vibrio cholerae and intestinal parasites among different age groups of hospitalized diarrhoeal patients in kolkata. a total of 3556 stool samples collected during 1996-2004 were screened for vibrios and parasites. the seasonality of v. cholerae and parasitic infections were studied in detail. the detection rates for ascaris lumbricoides and giardia lamblia infection were more than for the hook ... | 2008 | 17594735 |
| pressure and denaturants in the unfolding of triosephosphate isomerase: the monomeric intermediates of the enzymes from saccharomyces cerevisiae and entamoeba histolytica. | triosephosphate isomerase (tim) is a dimeric enzyme formed by two identical (beta/alpha)8 barrels. in this work, we compare the unfolding and refolding of the tims from entamoeba histolytica (ehtim) and baker's yeast (ytim). a monomeric intermediate was detected in the gdnhcl-induced unfolding of ehtim. the thermodynamic, spectroscopic, catalytic, and hydrodynamic properties of this intermediate were found to be very similar to those previously described for a monomeric intermediate of ytim obse ... | 2007 | 17595057 |
| the evolution of n-glycan-dependent endoplasmic reticulum quality control factors for glycoprotein folding and degradation. | asn-linked glycans (n-glycans) play important roles in the quality control (qc) of glycoprotein folding in the endoplasmic reticulum (er) lumen and in er-associated degradation (erad) of proteins by cytosolic proteasomes. a udp-glc:glycoprotein glucosyltransferase glucosylates n-glycans of misfolded proteins, which are then bound and refolded by calreticulin and/or calnexin in association with a protein disulfide isomerase. alternatively, an alpha-1,2-mannosidase (mns1) and mannosidase-like prot ... | 2007 | 17606910 |
| trichostatin a effects on gene expression in the protozoan parasite entamoeba histolytica. | histone modification regulates chromatin structure and influences gene expression associated with diverse biological functions including cellular differentiation, cancer, maintenance of genome architecture, and pathogen virulence. in entamoeba, a deep-branching eukaryote, short chain fatty acids (scfa) affect histone acetylation and parasite development. additionally, a number of active histone modifying enzymes have been identified in the parasite genome. however, the overall extent of gene reg ... | 2007 | 17612405 |
| intron length distributions and gene prediction. | accurate gene prediction in eukaryotes is a difficult and subtle problem. here we point out a useful feature of expected distributions of spliceosomal intron lengths. since introns are removed from transcripts prior to translation, intron lengths are not expected to respect coding frame, thus the number of genomic introns that are a multiple of three bases ('3n introns') should be similar to the number that are a multiple of three plus one bases (or plus two bases). skewed predicted intron lengt ... | 2007 | 17617639 |
| intranasal immunization with gal-inhibitable lectin plus an adjuvant of cpg oligodeoxynucleotides protects against entamoeba histolytica challenge. | the development of an effective amebiasis vaccine could improve child health in the developing world, reducing cases of amebic colitis and liver abscess. an ideal vaccine would be comprised of a well-characterized parasite antigen and an adjuvant, which would have high potency while driving the immune response in a th1 direction. this study describes a mucosal vaccine composed of the entamoeba histolytica galactose/n-acetyl-d-galactosamine-inhibitable lectin (gal-lectin) and cpg oligodeoxynucleo ... | 2007 | 17620349 |
| the use of the antifungal agent miconazole as an inhibitor of blastocystis hominis growth in entamoeba histolytica/e. dispar cultures. | in regions with high prevalence, blastocystis hominis is frequently found in association with entamoeba histolytica/e. dispar in xenic cultures. its exacerbated growth is often superimposed on the growth of amebas, thus impeding the continuation of the amebas in the culture, within a few generations. the present study reports on the excellent efficacy (100%) of the antifungal agent miconazole in eliminating b. hominis from cultures of e. histolytica/e. dispar, thereby maintaining the integrity o ... | 2007 | 17625701 |
| ehlima, a novel lim protein, localizes to the plasma membrane in entamoeba histolytica. | the parasitic protozoan entamoeba histolytica relies on a very dynamic cytoskeleton in order to invade and survive in host tissues. identification of cytoskeletal elements is key to understanding these processes. here we present the characterization of ehlima, the first lim protein of e. histolytica. ehlima consists of a single lim domain at its n terminus and exhibits the highest degree of homology with ddlime from dictyostelium discoideum. immunofluorescence localization of ehlima using anti-e ... | 2007 | 17630327 |
| laboratory diagnostic techniques for entamoeba species. | the genus entamoeba contains many species, six of which (entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, entamoeba polecki, entamoeba coli, and entamoeba hartmanni) reside in the human intestinal lumen. entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. although recent studies highlight the recovery of e. dispar and e. moshkovskii from patients with gastrointestinal symptoms, there is still no convin ... | 2007 | 17630338 |
| diarrhoea-associated parasites in hiv-1 seropositive and sero-negative patients in a teaching hospital, northwest ethiopia. | to determine the prevalence and type of intestinal parasites in hiv infected and uninfected patients with diarrhea. | 2007 | 17642173 |
| diarrhoeagenic escherichia coli and other causes of childhood diarrhoea: a case-control study in children living in a wastewater-use area in hanoi, vietnam. | a case-control study was conducted to identify the aetiology of diarrhoeal diseases in pre-school children in a suburban area of hanoi where the use of untreated wastewater in agriculture and aquaculture is a common practice. stool specimens and clinical information were collected from 111 pairs of children with diarrhoea and healthy controls. a total of 73 cases (66 %) and 41 controls (36 %) had an enteric pathogen. the pathogens most often associated with diarrhoea were rotavirus (17 % of case ... | 2007 | 17644717 |
| detection of cryptosporidium spp and other intestinal parasites in children with acute diarrhea and severe dehydration in rio de janeiro. | the objective of the present study was to estimate the frequency of infection by cryptosporidium spp and other intestinal parasites in dehydrated children with gastroenteritis who were admitted to a pediatric hospital. stool examinations from 218 children were performed. cryptosporidium spp was identified in eighteen out of 193 stool samples (9.3%) subjected to safranin-methylene blue staining. giardia lamblia was detected in ten out of 213 (4.7%) samples examined via the direct or ritchie metho ... | 2007 | 17653475 |
| non-biting cyclorrhaphan flies (diptera) as carriers of intestinal human parasites in slum areas of addis ababa, ethiopia. | a study was conducted to determine the role of non-biting cyclorrhaphan flies as carriers of intestinal parasites in slum areas of addis ababa from january 2004 to june 2004. a total of 9550 flies, comprising of at least seven species were collected from four selected sites and examined for human intestinal parasites using the formol-ether concentration method. the dominant fly species was chrysomya rufifacies (34.9%) followed by musca domestica (31%), musca sorbens (20.5.%), lucina cuprina (6.8 ... | 2007 | 17658447 |
| nitazoxanide in the treatment of amoebiasis. | amoebiasis is a significant cause of morbidity worldwide and is the third leading cause of death from parasitic diseases. this study evaluated nitazoxanide, a thiazolide anti-infective, in the treatment of intestinal and hepatic amoebiasis. prospective, randomised, double-blind, placebo-controlled studies were conducted in outpatients with intestinal amoebiasis from the nile delta of egypt. nitazoxanide was administered twice daily for 3 days at doses of 500mg (age > or =12 years), 200mg (age 4- ... | 2007 | 17658567 |
| [confirmation by pcr of the existence in tunisia of entamoeba histolytica and entamoeba dispar]. | amibias are illness in tunisia diagnosed until now on the sole basis of the morphological aspects of the parasite. our aim is to report the first tunisian results concerning the molecular identification of e. histolytica/e. dispar, | 2007 | 17668576 |
| computational study of glyceraldehyde-3-phosphate dehydrogenase of entamoeba histolytica: implications for structure-based drug design. | glyceraldehyde-3-phosphate dehydrogenase (gapdh) of the pathogenic protozoa entamoeba histolytica (eh) is a major glycolytic enzyme and an attractive drug target since this parasite lacks a functional citric acid cycle and is dependent solely on glycolysis for its energy requirements. the three-dimensional structure of dimeric ehgapdh in complex with cofactor nad(+) has been generated by homology modeling based on the crystal structure of human liver gapdh. our refined model indicates the presen ... | 2007 | 17676935 |
| nitroimidazole action in entamoeba histolytica: a central role for thioredoxin reductase. | metronidazole, a 5-nitroimidazole drug, has been the gold standard for several decades in the treatment of infections with microaerophilic protist parasites, including entamoeba histolytica. for activation, the drug must be chemically reduced, but little is known about the targets of the active metabolites. applying two-dimensional gel electrophoresis and mass spectrometry, we searched for protein targets in e. histolytica. of all proteins visualized, only five were found to form adducts with me ... | 2007 | 17676992 |
| comparison of multiplex-pcr and antigen detection for differential diagnosis of entamoeba histolytica. | amebiasis is an infection caused by entamoeba histolytica. however, differentiation between e. histolytica and entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. the purpose of the present study was to evaluate a multiplex -pcr for detection and differentiation of e. histolytica from e. dispar from fresh stool samples in comparison with the coproantigen commercial elisa. microscopic examination ... | 2007 | 17684641 |
| energetics of the native energy landscape of a two-domain calcium sensor protein: distinct folding features of the two domains. | the protein folding energy landscape allows a thorough understanding of the protein folding problem which in turn helps in understanding various aspects of biological functions. characterizing the cooperative unfolding units and the intermediates along the folding funnel of a protein is a challenging task. in this paper, we investigated the native energy landscape of ehcabp, a calcium sensor, belonging to the same ef-hand superfamily as calmodulin. ehcabp is a two-domain ef-hand protein consisti ... | 2007 | 17685557 |
| [autochthonous amebic liver abscess: microbiological diagnosis by pcr]. | | 2007 | 17692221 |
| diagnosis of intestinal amoebiasis by using nested polymerase chain reaction-restriction fragment length polymorphism assay. | microscopy is unreliable to distinguish the pathogenic entamoeba histolytica from the nonpathogenic entamoeba dispar or entamoeba moshkovskii in stool specimens. | 2007 | 17701126 |
| crosstalk at the initial encounter: interplay between host defense and ameba survival strategies. | the host-parasite relationship is based on a series of interplays between host defense mechanisms and parasite survival strategies. progress has been made in understanding the role of host immune response in amebiasis. while host cells elaborate diverse mechanisms for pathogen expulsion, amebae have also developed complex strategies to modulate host immune response and facilitate their own survival. this paper will give an overview of current research on the mutual interactions between host and ... | 2007 | 17702556 |
| novel structural and functional findings of the ehfln protein from entamoeba histolytica. | the ehfln protein (previously known as ehabp-120) is the first filamin to be identified in the parasitic protozoan entamoeba histolytica. filamins are a family of cross-linking actin-binding proteins that organize filamentous actin in networks and stress fibers. it has been reported that filamins of different organisms directly interact with more than 30 cellular proteins and some ppis. the biochemical consequences of such interactions may have either positive or negative effects on the cross-li ... | 2007 | 17705278 |
| acetylation of the entamoeba histone h4 n-terminal domain is influenced by short-chain fatty acids that enter trophozoites in a ph-dependent manner. | treatment of higher eukaryotic cells with short-chain fatty acids (scfa) such as butyrate causes decreased levels of histone deacetylase (hdac) activity and hyperacetylation of histones, and thereby affects gene expression, cell growth and differentiation. entamoeba parasites encounter high levels of scfa in the host colon, and in vitro these compounds allow trophozoite stage parasites to multiply but prevent their differentiation into infectious cysts. the entamoeba invadens ip-1 histone h4 pro ... | 2008 | 17706222 |
| molecular modeling on pyruvate phosphate dikinase of entamoeba histolytica and in silico virtual screening for novel inhibitors. | pyruvate phosphate dikinase (ppdk) is the key enzyme essential for the glycolytic pathway in most common and perilous parasite entamoeba histolytica. inhibiting the function of this enzyme could control the wide spread of intestinal infections caused by entamoeba histolytica in humans. with this objective, we modeled the three dimensional structure of the ppdk protein. we used templates with 51% identity and 67% similarity to employ homology-modeling approach. stereo chemical quality of protein ... | 2008 | 17710553 |
| the lysine- and glutamic acid-rich protein kerp1 plays a role in entamoeba histolytica liver abscess pathogenesis. | the parasite entamoeba histolytica colonizes the large bowel where it may persist as an asymptomatic luminal gut infection, which changes to virulence. parasite invasion of the intestine leads to dysentery and spreads to the liver, where amoebae form abscesses. we took advantage of changes in virulence that occurs after long-term in vitro culture of e. histolytica strains. using microarrays, we concluded that virulence correlates with upregulation of key genes involved in stress response, includ ... | 2008 | 17711481 |
| detection of diarrhoea-causing protozoa in general practice patients in the netherlands by multiplex real-time pcr. | the diagnostic value of a multiplex real-time pcr for the detection of entamoeba histolytica, giardia lamblia and cryptosporidium parvum/cryptosporidium hominis was evaluated by comparing the pcr results obtained with those of routinely performed microscopy of faecal samples from patients consulting their general practitioner (gp) because of gastrointestinal complaints. analysis of 722 faecal dna samples revealed that the prevalence of g. lamblia was 9.3% according to pcr, as compared to 5.7% by ... | 2007 | 17714523 |
| epidemiology of amoebic liver abscess in mexico: the case of sonora. | | 2007 | 17716437 |
| lipopopeptidephosphoglycan from entamoeba histolytica activates human macrophages and dendritic cells and reaches their late endosomes. | lipopopeptidephosphoglycan (lppg) is a complex macromolecule from the surface of entamoeba histolytica trophozoites. we analysed the interaction between lppg and human macrophages and dendritic cells (dcs) and found that lppg is internalized by these cells and activates them. the internalization process involves intracellular traffic from the cell membrane to late endosomes, as shown by co-localization of lppg with late endosomes marked with fitc-dextran and lamp-1. lppg-activated dcs have incre ... | 2007 | 17727570 |
| molecular and functional characterization of ehpak3, a p21 activated kinase from entamoeba histolytica. | p21-activated kinases (paks) are a family of serine/threonine kinases whose activity is regulated by the binding of the small rho family gtpases as well as by rhogtpase independent mechanisms. paks have wide-ranging functions which include cytoskeletal organisation, cell motility, cell proliferation and survival. we have identified a pak from entamoeba histolytica - ehpak3 that is distributed in the cytoplasm of unstimulated cells and localizes to the caps after induction of capping with concana ... | 2007 | 17761392 |
| identification and biochemical characterization of unique secretory nucleases of the human enteric pathogen, entamoeba histolytica. | the ancient eukaryotic human pathogen, entamoeba histolytica, is a nucleo-base auxotroph (i.e. lacks the ability to synthesize purines or pyrimidines de novo) and therefore is totally dependent upon its host for the supply of these essential nutrients. in this study, we identified two unique 28-kda, dithiothreitol-sensitive nucleases and showed that they are constitutively released/secreted by parasites during axenic culture. using several different molecular approaches, we identified and charac ... | 2007 | 17766245 |
| transcriptional gene silencing reveals two distinct groups of entamoeba histolytica gal/galnac-lectin light subunits. | the entamoeba histolytica cell surface gal/galnac-inhibitable lectin is a heterodimer between a heavy (170 kda) subunit linked via a disulfide bond to a light (31 to 35 kda) subunit. five light subunit genes with high homology have been identified (ehlgl1 to -5). we have previously shown that silencing of the expression of ehlgl1, in the g3 trophozoites which had already been silenced in the amoebapore gene (ehap-a), also suppressed the transcription of ehlgl2 and -3 (strain rbv). the total abse ... | 2007 | 17766462 |
| [transmission of enteropasitosis through currency notes]. | there are several ways to propagation of parasitary diseases and how there aren't many bibliographic work about this theme, it was realized a study near to the natal-rn population, to verify the enteroparasites transmission through the paper money. | 2005 | 17768817 |
| primary structure, expression and localization of two intermediate subunit lectins of entamoeba dispar that contain multiple cxxc motifs. | we have recently identified 2 surface proteins in entamoeba histolytica as intermediate subunits of galactose- and n-acetyl-d-galactosamine-inhibitable lectin (ehigl1 and ehigl2); these proteins both contain multiple cxxc motifs. here, we report the molecular characterization of the corresponding proteins in entamoeba dispar, which is neither pathogenic nor invasive. two igl genes encoding 1110 and 1106 amino acids (edigl1 and edigl2) were cloned from 2 strains of e. dispar. the amino acid seque ... | 2007 | 17803840 |
| kinetic modeling can describe in vivo glycolysis in entamoeba histolytica. | glycolysis in the human parasite entamoeba histolytica is characterized by the absence of cooperative modulation and the prevalence of pyrophosphate-dependent (over atp-dependent) enzymes. to determine the flux-control distribution of glycolysis and understand its underlying control mechanisms, a kinetic model of the pathway was constructed by using the software gepasi. the model was based on the kinetic parameters determined in the purified recombinant enzymes, and the enzyme activities, and st ... | 2007 | 17824961 |
| identification of an entamoeba histolytica serine-, threonine-, and isoleucine-rich protein with roles in adhesion and cytotoxicity. | entamoeba histolytica is a leading cause of parasitic death globally. however, the molecular framework regulating pathogenesis is poorly understood. we have previously used expression profiling to identify entamoeba genes whose expressions were strictly associated with virulent strains (r. c. macfarlane and u. singh, infect. immun. 74:340-351, 2006). one gene, which we have named ehstirp (entamoeba histolytica serine-, threonine-, and isoleucine-rich protein), was exclusively expressed in virule ... | 2007 | 17827347 |