| protective role of antibodies induced by brucella melitensis b115 against b. melitensis and brucella abortus infections in mice. | it has been demonstrated that antibodies specific for o-ps antigen of brucella smooth strains are involved in the protective immunity of brucellosis. since the rough strain brucella melitensis b115 was able to protect mice against wild brucella strains brucellosis despite the lack of anti-ops antibodies, in this study we evaluated the biological significance of antibodies induced by this strain, directed to antigens other than o-ps, passively tranferred to untreated mice prior to infection with ... | 2012 | 22521283 |
| the β-scaffold of the lov domain of the brucella light-activated histidine kinase is a key element for signal transduction. | light-oxygen-voltage (lov) domains are blue-light-activated signaling modules present in a wide range of sensory proteins. among them, the histidine kinases are the largest group in prokaryotes (lov-hk). light modulates the virulence of the pathogenic bacteria brucella abortus through lov-hk. one of the striking characteristic of brucella lov-hk is the fact that the protein remains activated upon light sensing, without recovering the basal state in the darkness. in contrast, the light state of t ... | 2012 | 22504229 |
| the virb-encoded type iv secretion system is critical for establishment of infection and persistence of brucella ovis infection in mice. | brucella spp. are gram-negative intracellular bacterial pathogens that cause chronic infections. brucella virulence factors include a type iv secretion system (t4ss) and its lipopolysaccharide (lps), which are essential for persistence. however, the role of the virb-encoded t4ss has not been investigated in naturally rough brucella species such as brucella ovis. in this study, male 6-week old balbc mice were infected with b. ovis, brucella abortus, and their respective δvirb2 mutant strains. dur ... | 2012 | 22483850 |
| vertical transmission of brucella abortus causes sterility in pregnant mice. | the mechanisms of abortion and sterility induced by bacterial infection are largely unknown. in the present study, we found that brucella abortus, a causative agent of brucellosis and facultative intracellular pathogen, caused sterility in pregnant mice. we have recently established a mouse model for abortion induced by b. abortus infection and high rates of abortion are observed for bacterial infection on day 4.5 of gestation, but not for other days. infected newborn (first generation) mice sho ... | 2012 | 22481220 |
| intradermal immunization with outer membrane protein 25 protects balb/c mice from virulent b. abortus 544. | brucella abortus is a causative agent of brucellosis, a zoonosis affecting the endemic areas, which infects domestic animals as well as humans, thus, posing a potential bioterror threat. outer membrane protein 25 is conserved among the brucella species. omp25 mutant strain of brucella is shown to be attenuated in mice emphasizing on the role of omp25 in brucella virulence. moreover, omp25 has been shown to inhibit tnf-α production in human macrophages, thereby, abrogating cell mediated immunity. ... | 2012 | 22464098 |
| safety and immunogenicity of brucella abortus strain rb51 vaccine in cross bred cattle calves in india. | safety and immunogenicity of brucella abortus rb51 vaccine has been evaluated in an organised dairy farm in india. all the cattle (r = 29) vaccinated with strain rb51 'responded' to the vaccine as demonstrated by ielisa using acetone killed strain rb51 antigen. the percentage responders at day 35, 60 and 90 post vaccination were 100%, 95% and 20%, respectively. strain rb51 was able to elicit a good ifn-gamma response from vaccinated animals. the post-vaccination time point analysis indicated tha ... | 2012 | 22439440 |
| rats born to brucella abortus infected mothers become latent carriers of brucella. | rats are known to be infected with brucella. vertical transmission of brucellosis was recorded in rats. the study was performed to judge whether rats born from brucella abortus infected mothers can act as latent carriers of brucella infection. | 2012 | 22421607 |
| identification of genes contributing to the intracellular replication of brucella abortus within hela and raw 264.7 cells. | brucella abortus, the causative agent of brucellosis, can survive and replicate within host cells. understanding bacterial virulence factors and bacteria-host cell interactions is critical for controlling brucellosis, yet very little is known about the virulence strategies and signaling pathways activated in phagocytes during infection to ensure their growth and survival. b. abortus was mutagenized by mini-tn5km2 transposon mutagenesis to identify virulence genes related to the internalization a ... | 2012 | 22397928 |
| brucella abortus induces apoptosis of human t lymphocytes. | immune evasion is essential for brucella abortus to survive in the face of robust adaptive cd4+ t cell response. we have previously demonstrated that b. abortus can indirectly inhibit cd4+ t cells by down-regulating mhc-ii expression and antigen presentation on macrophages. however, whether b. abortus is able to directly interfere with t lymphocytes is not known. we report here that b. abortus induces apoptosis of human t lymphocytes, even though invasion of t lymphocytes was low and non-replica ... | 2012 | 22387699 |
| polymeric display of proteins through high affinity leucine zipper peptide adaptors. | the polymeric display of proteins is a method that could be used to increase the immunogenicity of antigens and to enhance the interaction strength of binding domains for their target ligands through an avidity effect. however, the coupling of proteins to oligomeric scaffolds is challenging. the chemical conjugation and recombinant fusion techniques have limitations that prevent their general use. in this work we describe a simple and effective method for coupling proteins to the decameric struc ... | 2012 | 22372794 |
| investigation of rifampicin resistance mechanisms in brucella abortus using ms-driven comparative proteomics. | mutations in the rpob gene have already been shown to contribute to rifampicin resistance in many bacterial strains including brucella species. resistance against this antibiotic easily occurs and resistant strains have already been detected in human samples. we here present the first research project that combines proteomic, genomic, and microbiological analysis to investigate rifampicin resistance in an in vitro developed rifampicin resistant strain of brucella abortus 2308. in silico analysis ... | 2012 | 22360387 |
| serological and bacteriological responses of water buffalo (bubalus bubalis) vaccinated with two doses of brucella abortus strain rb51 vaccine. | thirty-two water buffalo (bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to brucella abortus strain rb51 (rb51) vaccination in a dose-response study and to compare the use of two selective media for the isolation of rb51. the animals were randomly divided into three treatment groups. groups i-iii received the recommended vaccine dose (rd) twice 4 weeks apart, rd twice 18 weeks apart and saline once, respectively. lymph nodes were excised from the three group ... | 2012 | 22297422 |
| serological profile of buffalo (bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose bengal, 2-mercaptoethanol and complement fixation tests. | the serological profiles of 21 female buffaloes vaccinated between 3 and 8 months of age using brucella abortus strain 19 (s19) were evaluated by rose bengal (rbt), 2-mercaptoethanol (2me) and complement fixation (cft) tests. the serum strains were collected in day zero, 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. no animal showed reaction in day zero. in 15th day above 95% of animals revealed reaction in all tests. all the animals presented absen ... | 2012 | 22284623 |
| identification and functional analysis of the cyclopropane fatty acid synthase of brucella abortus. | the brucellae are facultative intracellular pathogens of mammals that are transmitted by contact with infected animals or contaminated materials. several major lipidic components of the brucella cell envelope are imperfectly recognized by innate immunity, thus contributing to virulence. these components carry large proportions of acyl chains of lactobacillic acid, a long chain cyclopropane fatty acid (cfa). cfas result from addition of a methylene group to unsaturated acyl chains and contribute ... | 2012 | 22262102 |
| heterophil/lymphocyte ratios predict the magnitude of humoral immune response to a novel antigen in great tits (parus major). | animals display remarkable individual variation in their capacity to mount immune responses against novel antigens. according to the life-history theory, this variation is caused by the costs of immune responses to the hosts. we studied one of such potential costs, depletion of somatic resources in wintering wild-caught captive passerines, the great tits (parus major) by immune challenging the birds with a novel antigen, killed brucella abortus (ba) suspension. we found that despite mild tempera ... | 2012 | 22245489 |
| role of tlrs in brucella mediated murine dc activation in vitro and clearance of pulmonary infection in vivo. | brucellosis is worldwide zoonoses affecting 500,000 people annually with no approved human vaccines available. live attenuated brucella abortus vaccine strain rb51 protects cattle through cd4 and cd8 t-cell mediated responses. however, limited information is known regarding how brucella stimulate innate immunity. although the most critical toll like receptors (tlrs) involved in the recognition of brucella are tlr2, tlr4 and tlr9, it is important to identify the essential tlrs that induce dc acti ... | 2012 | 22234268 |
| mouse cytokine profile skewed towards th2 in pregnancy during infection with brucella abortus s19 strain. | the two classes of cytokines th1 and th2 determine the type of immune response elicited. the th2 immune response is associated with successful pregnancy. brucellosis is an intracellular bacterium that elicits the th1 response and is known to cause spontaneous abortion in mammalian species. this study sought to determine if brucella infection causes spontaneous abortion by causing the circulating cytokine profile be th1 dominant during pregnancy. forty-eight swiss white mice were used in this mur ... | 2011 | 25566611 |
| a case of brucellosis presenting with severe thrombocytopenia. | thrombocytopenia is one of the rare hematologic complication of brucellosis. herein a case of brucellosis with severe thrombocytopenia was reported. the patient was admitted to hematology service with epistaxis, ecchymoses, fever, thrombocytopenia (0.6 x 103/μl) and anemia. his initial diagnosis was idiopathic thrombocytopenic purpura. corticosteroid treatment was started and erythrocyte and platelet suspensions were transfused. despite this treatment clinical symptoms and laboratory disorders w ... | 2003 | 27265335 |
| enhanced uptake of liposomes by bovine macrophages after opsonization with antibodies to brucella abortus. | phosphatidylcholine:cholesterol liposomes containing 4(5) carboxyfluorescein were modified by incorporation of brucella abortus lipopolysaccharide. these vesicles were opsonized with bovine anti-b abortus or anti-b abortus plus complement. the fluorescent marker entrapped in antibody-opsonized liposomes was taken up by cultured bovine macrophages more effectively than was the same compound which had been loaded into nonopsonized liposomes. the addition of bovine complement to antibody-opsonized ... | 1984 | 24049908 |
| secondary infection of a total hip replacement with brucella abortus. | a 54-year-old dairy farmer developed systemic manifestations of brucellosis five months following total hip replacement. initial medical treatment with tetracycline relieved his systemic symptoms; however, hip symptoms persisted up to two years later. at surgery, a culture of the hip grew brucella abortus. subsequent treatment with parenteral antibiotics and a one-stage total hip revision utilizing streptomycin-impregnated cement was performed. the patient has remained symptom free at 36 months ... | 1983 | 24823202 |
| survey of colorado's wild ruminants for serologic titers to brucellosis and leptospirosis. | a 10-year survey for serologic titers to brucellosis and leptospirosis in mule deer ( odocoileus hemionus ), elk ( cervus canadensis ), and antelope ( antilocapra americana ) in colorado is summarized. over 10,000 blood samples were tested against brucella abortus and 4,747 samples were tested against leptospira pomona . a total of 1,761 blood samples were tested against l. canicola , l. grippotyphosa , l. hardjo , l. icterohemorrhagiae . all results were considered negative. | 1977 | 24228967 |
| a plate method of antibiotic assay with mycobacterium tuberculosis 607 and brucella abortus. | | 1951 | 24540608 |
| [relations between experimental infection with brucella abortus and avitaminosis b1 and c]. | | 2014 | 24537704 |
| [undulant fever in human and brucella abortus in domestic animals in bulgaria]. | | 1949 | 24537554 |
| brucella melitensis methionyl-trna-synthetase (metrs), a potential drug target for brucellosis. | we investigated brucella melitensis methionyl-trna-synthetase (bmmetrs) with molecular, structural and phenotypic methods to learn if bmmetrs is a promising target for brucellosis drug development. recombinant bmmetrs was expressed, purified from wild type brucella melitensis biovar abortus 2308 strain atcc/crp #dd-156 and screened by a thermal melt assay against a focused library of one hundred previously classified methionyl-trna-synthetase inhibitors of the blood stage form of trypanosoma bru ... | 2016 | 27500735 |
| serology and protein electrophoresis for evidence of exposure to 12 mink pathogens in free-ranging american mink (neovison vison) in argentina. | basic pathologic characteristics for farmed minks were previously reported worldwide. however, its status in the wild has not been studied in detail. | 2017 | 28635524 |
| a brucella type iv effector targets the cog tethering complex to remodel host secretory traffic and promote intracellular replication. | many intracellular pathogens exploit host secretory trafficking to support their intracellular cycle, but knowledge of these pathogenic processes is limited. the bacterium brucella abortus uses a type iv secretion system (virb t4ss) to generate a replication-permissive brucella-containing vacuole (rbcv) derived from the host er, a process that requires host early secretory trafficking. here we show that the virb t4ss effector bspb contributes to rbcv biogenesis and brucella replication by intera ... | 2017 | 28844886 |
| trypanosoma infection favors brucella elimination via il-12/ifnγ-dependent pathways. | this study develops an original co-infection model in mice using brucella melitensis, the most frequent cause of human brucellosis, and trypanosoma brucei, the agent of african trypanosomiasis. although the immunosuppressive effects of t. brucei in natural hosts and mice models are well established, we observed that the injection of t. brucei in mice chronically infected with b. melitensis induces a drastic reduction in the number of b. melitensis in the spleen, the main reservoir of the infecti ... | 2017 | 28824630 |
| the bactericidal effect of high temperature is an essential resistance mechanism of chicken macrophage against brucella abortus infection. | knowledge of avian host responses to brucellosis is critical to understanding how avian resists this infection however this mechanism is not well established. on the other hand, temperature has a major involvement in the physiology of living organisms and cell death induced by heat is attributed to protein denaturation. this study demonstrates the direct bactericidal effect of a higher temperature (41ºc) to brucella (b.) abortus that resulted in the gradual reduction of intracellular bacteria th ... | 2017 | 28813778 |
| safety of vaccination against brucellosis with the rough strain in pregnant cattle. | brucellosis is an infectious and contagious disease that profoundly impacts public health. however, in many countries, disease prevention is restricted to the vaccination of calves, and there is no prophylactic strategy for pregnant heifers and cows. the aim of this study was to evaluate the safety of the rough strain vaccine against brucellosis in pregnant cattle. crossbred cows (n = 96) at three gestational periods (early, mid, or late pregnancy) were randomly allocated into the vaccine treatm ... | 2017 | 28812192 |
| epidemiology of bovine brucellosis in costa rica: lessons learned from failures in the control of the disease. | brucellosis, caused by brucella abortus is a major disease of cattle and a zoonosis. in order to estimate the bovine brucellosis prevalence in costa rica (cr), a total 765 herds (13078 bovines) from six regions of cr were randomly sampled during 2012-2013. a non-random sample of 7907 herds (532199 bovines) of the six regions, arriving for diagnoses during 2014-2016 to the costa rican animal health service was also studied. the prevalence estimated by rose bengal test (rbt) ranged from 10.5%-11.4 ... | 2017 | 28797045 |
| b. abortus rna is the component involved in the down-modulation of mhc-i expression on human monocytes via tlr8 and the egfr pathway. | despite eliciting a potent cd8+ t cell response, brucella abortus is able to persist and establish a chronic infection inside its host. we have previously reported that the infection of human monocytes/macrophages with b. abortus inhibits the ifn-γ-induced mhc-i cell surface expression down-modulating cytotoxic cd8+ t cell responses. mhc-i down-modulation depends on bacterial viability and results from the capacity of b. abortus to retain the mhc-i molecules within the golgi apparatus. furthermo ... | 2017 | 28767704 |
| using real-time polymerase chain reaction as an alternative rapid method for enumeration of colony count in live brucella vaccines. | brucellosis is a major bacterial zoonosis of global importance affecting a range of animal species and man worldwide. it has economic, public health, and bio-risk importance. control and prevention of animal brucellosis mainly depend on accurate diagnostic tools and implementation of effective and safe animal vaccination program. there are three types of animal brucella live vaccines - brucella melitensis rev-1 vaccine, brucella abortus s19, and b. abortus rb51. evaluation of these vaccines depe ... | 2017 | 28717311 |
| brucella infection in asian sea otters (enhydra lutris lutris) on bering island, russia. | infection with brucella spp., long known as a cause of abortion, infertility, and reproductive loss in domestic livestock, has increasingly been documented in marine mammals over the past two decades. we report molecular evidence of brucella infection in asian sea otters (enhydra lutris lutris). brucella dna was detected in 3 of 78 (4%) rectal swab samples collected between 2004 and 2006 on bering island, russia. these 78 animals had previously been documented to have a brucella seroprevalence o ... | 2017 | 28715292 |
| exploring the diversity of field strains of brucella abortus biovar 3 isolated in west africa. | brucellosis is one of the most widespread bacterial zoonotic diseases in the world, affecting both humans and domestic and wild animals. identification and biotyping of field strains of brucella are of key importance for a better knowledge of the epidemiology of brucellosis, for identifying appropriate antigens, for managing disease outbreaks and for setting up efficient preventive and control programmes. such data are required both at national and regional level to assess potential threats for ... | 2017 | 28713359 |
| tannic acid-mediated immune activation attenuates brucella abortus infection in mice. | brucellosis is an emerging infectious disease affecting humans and animals. in this study, we investigated the in vitro and in vivo effects of tannic acid (ta) against brucella (b.) abortus infection. reduced f-actin polymerization and mapks (erk 1/2 and p38α) phosphorylation were observed in ta-treated cells as compared with control cells after infection. the mice were infected via intraperitoneal route and were orally given with ta or pbs for 14 days. the weights of the spleens from ta-treated ... | 2017 | 28693306 |
| proline utilization system is required for infection by the pathogenic α-proteobacterium brucella abortus. | proline utilization (put) systems have been described in a number of bacteria; however, the importance and functionality of the put system in the intracellular pathogen brucellaabortus has not been explored. generally, bacterial put systems are composed of the bifunctional enzyme proline dehydrogenase puta and its transcriptional activator putr. here, we demonstrate that the genes puta (bab2_0518) and putr (bab2_0517) are critical for the chronic infection of mice by b. abortus, but puta and put ... | 2017 | 28691659 |
| serological survey of toxoplasmosis, neosporosis and brucellosis among cattle herds in oyo state, south-western nigeria. | several zoonotic diseases are known to constitute great impediment to livestock management and production worldwide, especially in developing countries where control measures are largely non-existent. this study sets out to investigate the occurrence of toxoplasmosis, neosporosis and brucellosis among cattle herds in oyo state, southwest nigeria. | 2017 | 28670646 |
| occurrence of oxidative stress in dairy cows seropositives for brucella abortus. | bovine brucellosis is an important zoonotic disease caused by the bacterium brucella abortus that leads to economic losses due to animal discard and commercial restrictions. since positive animals for brucellosis are culled, little is known about the pathogenesis of this disease. therefore, the aims of this study were to evaluate possible changes in the activity of deaminase adenosine (ada) and the oxidative stress in cows seropositives for brucellosis (experiment i), and to evaluate the seropre ... | 2017 | 28669636 |
| performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of brucella abortus in semen and serum of bovine bulls. | brucellosis remains as a major infectious disease of domestic animals and is considered a re-emerging zoonosis in several countries. b. abortus infections in bulls are related to reproductive tract infections, although infected animals show transient serological titers or nonreactor status. thus, diagnosis of bovine brucellosis based exclusively on serological tests probably underestimates b. abortus infections in bulls. in this scenario, three hundred thirty-five serum samples from reproductive ... | 2017 | 28666718 |
| development of a forecasting model for brucellosis spreading in the italian cattle trade network aimed to prioritise the field interventions. | brucellosis caused by brucella abortus is an important zoonosis that constitutes a serious hazard to public health. prevention of human brucellosis depends on the control of the disease in animals. livestock movement data represent a valuable source of information to understand the pattern of contacts between holdings, which may determine the inter-herds and intra-herd spread of the disease. the manuscript addresses the use of computational epidemic models rooted in the knowledge of cattle trade ... | 2017 | 28654703 |
| th2-related immune responses by the brucella abortus cellular antigens, malate dehydrogenase, elongation factor, and arginase. | brucellosis is an important zoonotic disease caused by brucella species. the disease is difficult to control due to the intracellular survival of the bacterium and the lack of precise understanding of pathogenesis. despite of continuous researches on the pathogenesis of brucella spp. infection, there is still question on the pathogenesis, especially earlier immune response in the bacterial infection. malate dehydrogenase (mdh), elongation factor (tsf), and arginase (rocf), which showed serologic ... | 2017 | 28629726 |
| brucella lipopolysaccharide reinforced salmonella delivering brucella immunogens protects mice against virulent challenge. | intracellular pathogen salmonella exhibits natural infection broadly analogous to brucella, this phenomenon makes salmonella a pragmatic choice for an anti-brucella vaccine delivery platform. in this study we developed and formulated a combination of four attenuated salmonella typhimurium live vector strains delivering heterologous brucella antigens (rbs), namely lumazine synthase, proline racemase subunit a, lipoprotein outer membrane protein-19, and cu-zn superoxide dismutase. with an aim to d ... | 2017 | 28622869 |
| shifting brucellosis risk in livestock coincides with spreading seroprevalence in elk. | tracking and preventing the spillover of disease from wildlife to livestock can be difficult when rare outbreaks occur across large landscapes. in these cases, broad scale ecological studies could help identify risk factors and patterns of risk to inform management and reduce incidence of disease. between 2002 and 2014, 21 livestock herds in the greater yellowstone area (gya) were affected by brucellosis, a bacterial disease caused by brucella abortus, while no affected herds were detected betwe ... | 2017 | 28609437 |
| highly sensitive bacteriophage-based detection of brucella abortus in mixed culture and spiked blood. | for decades, bacteriophages (phages) have been used for brucella species identification in the diagnosis and epidemiology of brucellosis. traditional brucella phage typing is a multi-day procedure including the isolation of a pure culture, a step that can take up to three weeks. in this study, we focused on the use of brucellaphages for sensitive detection of the pathogen in clinical and other complex samples, and developed an indirect method of brucella detection using real-time quantitative pc ... | 2017 | 28604602 |
| a 6-nucleotide regulatory motif within the abcr small rnas of brucella abortus mediates host-pathogen interactions. | in brucella abortus, two small rnas (srnas), abcr1 and abcr2, are responsible for regulating transcripts encoding abc-type transport systems. abcr1 and abcr2 are required for brucella virulence, as a double chromosomal deletion of both srnas results in attenuation in mice. although these srnas are responsible for targeting transcripts for degradation, the mechanism utilized by the abcr srnas to regulate mrna in brucella has not been described. here, two motifs (m1 and m2) were identified in abcr ... | 2017 | 28588127 |
| brucella abortus induces a warburg shift in host metabolism that is linked to enhanced intracellular survival of the pathogen. | intracellular bacterial pathogens exploit host cell resources to replicate and survive inside the host. targeting these host systems is one promising approach to developing novel antimicrobials to treat intracellular infections. we show that human macrophage-like cells infected with brucella abortus undergo a metabolic shift characterized by attenuated tricarboxylic acid cycle metabolism, reduced amino acid consumption, altered mitochondrial localization, and increased lactate production. this s ... | 2017 | 28559292 |
| a comprehensive proteogenomic study of the human brucella vaccine strain 104 m. | brucella spp. are gram-negative, facultative intracellular pathogens that cause brucellosis in both humans and animals. the b. abortus vaccine strain 104 m is the only vaccine available in china for the prevention of brucellosis in humans. although the b. abortus 104 m genome has been fully sequenced, the current genome annotations are not yet complete. in addition, the main mechanisms underpinning its residual toxicity and vaccine-induced immune protection have yet to be elucidated. mapping the ... | 2017 | 28535754 |
| evaluation of plasma sphingosine 1-phosphate, hepcidin and cardiovascular damage biomarkers (cardiac troponin i and homocysteine) in rats infected with brucellosis and vaccinated (rev-1, rb-51). | brucellosis is known as one of important zoonosis. studying the histological and biochemical effects of the disease could help to increase our knowledge about it. the aim of the present study was to evaluate changes of plasma parameters after intraperitoneal injection of two species of brucella (brucella melitensis and brucella abortus) and two vaccines (rev-1, rb-51) in the rat. forty male rats were divided into five groups (n = 8 in each group). two groups received suspensions of brucella abor ... | 2017 | 28533142 |
| getting "inside" type i ifns: type i ifns in intracellular bacterial infections. | type i interferons represent a unique and complex group of cytokines, serving many purposes during innate and adaptive immunity. discovered in the context of viral infections, type i ifns are now known to have myriad effects in infectious and autoimmune disease settings. type i ifn signaling during bacterial infections is dependent on many factors including whether the infecting bacterium is intracellular or extracellular, as different signaling pathways are activated. as such, the repercussions ... | 2017 | 28529959 |
| estimating loss of brucella abortus antibodies from age-specific serological data in elk. | serological data are one of the primary sources of information for disease monitoring in wildlife. however, the duration of the seropositive status of exposed individuals is almost always unknown for many free-ranging host species. directly estimating rates of antibody loss typically requires difficult longitudinal sampling of individuals following seroconversion. instead, we propose a bayesian statistical approach linking age and serological data to a mechanistic epidemiological model to infer ... | 2017 | 28508154 |
| molecular investigation of virulence factors of brucella melitensis and brucella abortus strains isolated from clinical and non-clinical samples. | brucella is zoonotic pathogen that induces abortion and sterility in domestic mammals and chronic infections in humans called malta fever. it is a facultative intracellular potential pathogen with high infectivity. the virulence of brucella is dependent upon its potential virulence factors such as enzymes and cell envelope associated virulence genes. the aim of this study was to investigate the brucella virulence factors among strains isolated from humans and animals in different parts of iran. ... | 2017 | 28506887 |
| incidence and knowledge of bovine brucellosis in kahuro district, murang'a county, kenya. | brucellosis in cattle is a zoonosis mainly caused by brucella abortus. in kenya, the disease is widespread, but its prevalence is largely unknown. the objective of this study was to investigate incidence rates of brucellosis and farmers' knowledge on the disease in kahuro district, murang'a county. in this study, 150 pooled milk samples were collected from 75 milk collection centers and tested. subsequently, 230 milk samples were collected from farmers in 16 collection centers in wangu and mugoi ... | 2017 | 28466240 |
| in a mouse model of sepsis, hepcidin ablation ameliorates anemia more effectively than iron and erythropoietin treatment. | intensive care unit (icu) anemia is an extreme version of anemia of inflammation (ai) that occurs commonly in critically ill patients and is associated with increased morbidity and mortality. currently available therapies for icu anemia have shown inconsistent efficacies in clinical trials. we conducted a systematic study of the effects of early versus delayed iron (fe) and/or erythropoietin (epo) therapy in our previously characterized mouse model of icu anemia based on an injection of heat-kil ... | 2017 | 28452907 |
| a novel pcr assay for detecting brucella abortus and brucella melitensis. | brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. the classical bacteriological detection process used to identify brucella spp. is difficult and time-consuming. this study aimed to develop a novel molecular assay for detecting brucellosis. | 2017 | 28443226 |
| brucella abortus-activated microglia induce neuronal death through primary phagocytosis. | inflammation has long been implicated as a contributor to pathogenesis in neurobrucellosis. many of the associated neurocognitive symptoms of neurobrucellosis may be the result of neuronal dysfunction resulting from the inflammatory response induced by brucella abortus infection in the central nervous system. in this manuscript, we describe an immune mechanism for inflammatory activation of microglia that leads to neuronal death upon b. abortus infection. b. abortus was unable to infect or harm ... | 2017 | 28398652 |
| protein/protein, dna/dna and dna/protein based vaccination strategies using truncated omp2b against brucella infection in balb/c mice. | the purpose of the present study was to evaluate the immunogenicity and protective efficacy of the truncated form of outer membrane protein 2b (tomp2b) from brucella abortus in balb/c mice. three immunization regimens protein/protein, dna/dna and dna/protein were used. immunization of mice with all vaccine strategies elicited a strong specific igg responses (igg2a titers over igg1) and provided t helper1 (th1) oriented immune responses. furthermore, protein/protein (pro/pro-) and dna/pro- vaccin ... | 2017 | 28391992 |