| an attenuated mycobacterium tuberculosis clinical strain with a defect in esx-1 secretion induces minimal host immune responses and pathology. | although mycobacterium tuberculosis (m.tb) dk9897 is an attenuated strain, it was isolated from a patient with extrapulmonary tuberculosis and vaccination with a subunit vaccine (h56) induced poor protection against it. both attenuation and lack of protection are because m.tb dk9897 cannot secrete the esxa virulence factor nor induce a host response against it. genome sequencing identified a frameshift mutation in the eccca1 gene. since the encoded eccca1 protein provides energy for esx-1 secret ... | 2017 | 28436493 |
| functional, thermodynamics, structural and biological studies of in silico-identified inhibitors of mycobacterium tuberculosis enoyl-acp(coa) reductase enzyme. | novel chemotherapeutics agents are needed to kill mycobacterium tuberculosis, the main causative agent of tuberculosis (tb). the m. tuberculosis 2-trans-enoyl-acp(coa) reductase enzyme (mtinha) is the druggable bona fide target of isoniazid. new chemotypes were previously identified by two in silico approaches as potential ligands to mtinha. the inhibition mode was determined by steady-state kinetics for seven compounds that inhibited mtinha activity. dissociation constant values at different te ... | 2017 | 28436453 |
| il-10 down-regulates the expression of survival associated gene hspx of mycobacterium tuberculosis in murine macrophage. | mycobacterium tuberculosis (mtb) adopts a special survival strategy to overcome the killing mechanism(s) of host immune system. amongst the many known factors, small heat shock protein 16.3 (shsp16.3) of mtb encoded by gene hspx has been reported to be critical for the survival of mtb. in the present study, the effect of recombinant murine interferon-gamma (rmifn-γ) and recombinant murine interleukin-10 (rmil-10) on the expression of gene hspx of mtb in murine macrophage raw264.7 has been invest ... | 2017 | 28435012 |
| defining a research agenda to address the converging epidemics of tuberculosis and diabetes: part 2: underlying biologic mechanisms. | there is growing interest in the re-emerging interaction between type 2 diabetes (dm) and tb, but the underlying biologic mechanisms are poorly understood despite their possible implications in clinical management. experts in epidemiologic, public health, basic science, and clinical studies recently convened and identified research priorities for elucidating the underlying mechanisms for the co-occurrence of tb and dm. we identified gaps in current knowledge of altered immunity in patients with ... | 2017 | 28434937 |
| distant phe345 mutation compromises the stability and activity of mycobacterium tuberculosis isocitrate lyase by modulating its structural flexibility. | isocitrate lyase (icl), a potential anti-tubercular drug target, catalyzes the first step of the glyoxylate shunt. in the present investigation, we studied the conformational flexibility of mtbicl to better understand its stability and catalytic activity. our biochemical results showed that a point mutation at phe345, which is topologically distant (>10 å) to the active site signature sequence ((189)kkcgh(193)), completely abolishes the activity of the enzyme. in depth computational analyses wer ... | 2017 | 28432345 |
| mycobacterium tuberculosis lineage distribution in xinjiang and gansu provinces, china. | mycobacterium tuberculosis (m. tuberculosis) genotyping has dramatically improved the understanding of the epidemiology of tuberculosis (tb). in this study, 187 m. tuberculosis isolates from xinjiang uygur autonomous region (xinjiang) and gansu province in china were genotyped using large sequence polymorphisms (lsps) and variable number tandem repeats (vntr). ten isolates, which represent major nodes of vntr-based minimum spanning tree, were selected and subsequently subjected to multi-locus se ... | 2017 | 28432321 |
| suppressors of cytokine signaling in tuberculosis. | tuberculosis (tb), a global disease mainly infected by mycobacterium tuberculosis, remains leading public health problem worldwide. suppressors of cytokine signaling (socss) play important roles in the protection against microbial infection. however, the relationship between members of the socs family and tuberculosis infection remains unclear. using peripheral blood mononuclear cells, we investigated the mrna expression profiles of socs subfamilies among active tb, latent tuberculosis infection ... | 2017 | 28430824 |
| diagnosis of smear-negative tuberculosis is greatly improved by xpert mtb/rif. | diagnosis of pulmonary (ptb) and extra-pulmonary tuberculosis (eptb) in smear-negative patients can be difficult. we assessed retrospectively the performance of xpert mtb/rif system (xpert, cepheid) in diagnosing smear-negative tuberculosis (tb), which represents the most common form of tb in a low incidence setting. | 2017 | 28430807 |
| bedaquiline susceptibility test for totally drug-resistant tuberculosis mycobacterium tuberculosis. | this study aimed to provide information that bedaquilline is significantly effective for treatment of totally drug resistant (tdr) mycobacterium tuberculosis that shows resistant to all first- and second-line drugs-using an innovative disc agarose channel (dac) system. time-lapse images of single bacterial cells under culture conditions with different concentrations of bedaquiline were analysed by image processing software to determine minimum inhibitory concentrations (mics). bedaquiline inhibi ... | 2017 | 28429168 |
| dihydrolipoamide dehydrogenase-lpd (rv0462)-specific t cell recall responses are higher in healthy household contacts of tb: a novel immunodominant antigen from m. tuberculosis. | the partial effectiveness against pulmonary tuberculosis (ptb), displayed by the existing tuberculosis (tb) vaccine, bacillus calmette-guérin (bcg), highlights the need for novel vaccines to replace or improve bcg. in tb immunology, antigen-specific cellular immune response is frequently considered indispensable. latency-associated antigens are intriguing as targets for tb vaccine development. the mycobacterial protein, dihydrolipoamide dehydrogenase (lpd; rv0462), the third enzyme of the pyruva ... | 2017 | 28428201 |
| novel and revisited approaches in antituberculosis drug discovery. | the increasing prevalence of multidrug-resistant mycobacterium tuberculosis (mtb) necessitates the discovery and development of novel drugs against tuberculosis. in this review, we focus on two recent approaches that led to the discovery of promising antitubercular compound classes: (i) hits derived from large compound library screens are increasingly difficult to translate into clinical application; this in turn fostered the development of innovative screening methods. (ii) an alternative strat ... | 2017 | 28427007 |
| rapid detection of mycobacterium tuberculosis and rifampicin resistance in extrapulmonary tuberculosis and sputum smear-negative pulmonary suspects using xpert mtb/rif. | tuberculosis (tb) is a serious public health problem in developing countries such as pakistan. rapid diagnosis of tb and detection of drug resistance are very important for timely and appropriate management of multidrug-resistant tb (mdr-tb). | 2017 | 28425873 |
| mycobacterium tuberculosis resistance prediction and lineage classification from genome sequencing: comparison of automated analysis tools. | whole-genome sequencing (wgs) has the potential to accelerate drug-susceptibility testing (dst) to design appropriate regimens for drug-resistant tuberculosis (tb). several recently developed automated software tools promise to standardize the analysis and interpretation of wgs data. we assessed five tools (castb, kvarq, mykrobe predictor tb, phyresse, and tbprofiler) with regards to dst and phylogenetic lineage classification, which we compared with phenotypic dst, sanger sequencing, and tradit ... | 2017 | 28425484 |
| data intensive genome level analysis for identifying novel, non-toxic drug targets for multi drug resistant mycobacterium tuberculosis. | we report the construction of a novel systems biology based virtual drug discovery model for the prediction of non-toxic metabolic targets in mycobacterium tuberculosis (mtb). this is based on a data-intensive genome level analysis and the principle of conservation of the evolutionarily important genes. in the 1623 sequenced mtb strains, 890 metabolic genes identified through a systems approach in mtb were evaluated for non-synonymous mutations. the 33 genes showed none or one variation in the e ... | 2017 | 28425478 |
| interactions between type 1 interferons and the th17 response in tuberculosis: lessons learned from autoimmune diseases. | the classical paradigm of tuberculosis (tb) immunity, with a central protective role for th1 responses and ifn-γ-stimulated cellular responses, has been challenged by unsatisfactory results of vaccine strategies aimed at enhancing th1 immunity. moreover, preclinical tb models have shown that increasing ifn-γ responses in the lungs is more damaging to the host than to the pathogen. type 1 interferon signaling and altered th17 responses have also been associated with active tb, but their functiona ... | 2017 | 28424682 |
| the within-host population dynamics of mycobacterium tuberculosis vary with treatment efficacy. | combination therapy is one of the most effective tools for limiting the emergence of drug resistance in pathogens. despite the widespread adoption of combination therapy across diseases, drug resistance rates continue to rise, leading to failing treatment regimens. the mechanisms underlying treatment failure are well studied, but the processes governing successful combination therapy are poorly understood. we address this question by studying the population dynamics of mycobacterium tuberculosis ... | 2017 | 28424085 |
| erratum to: rhodococcus erythropolis as a host for expression, secretion and glycosylation of mycobacterium tuberculosis proteins. | | 2017 | 28424079 |
| zwitterionic pyrrolidene-phosphonates: inhibitors of the glycoside hydrolase-like phosphorylase streptomyces coelicolor glgei-v279s. | we synthesized and evaluated new zwitterionic inhibitors against glycoside hydrolase-like phosphorylase streptomyces coelicolor (sco) glgei-v279s which plays a role in α-glucan biosynthesis. sco glgei-v279s serves as a model enzyme for validated anti-tuberculosis (tb) target mycobacterium tuberculosis (mtb) glge. pyrrolidine inhibitors 5 and 6 were designed based on transition state considerations and incorporate a phosphonate on the pyrrolidine moiety to expand the interaction network between t ... | 2017 | 28422240 |
| accurate and effective multidrug-resistant mycobacterium tuberculosis detection method using gap-filling ligation coupled with high-resolution capillary electrophoresis-based single strand conformation polymorphism. | tuberculosis (tb) has severely threatened public health via emerging multidrug-resistant (mdr) and extensively drug-resistant (xdr) mycobacterium tuberculosis (mtb) strains. for effective tb treatment, rapid, accurate, and multiplex detection of drug resistance is extremely important. however, conventional methods for tb diagnosis are time consuming and have a limited effect on treatment. nucleic acid-based molecular detection methods have been developed as an effective mdr/xdr-tb diagnosis tech ... | 2017 | 28422112 |
| high detection rates of urine mycobacterium tuberculosis in patients with suspected miliary tuberculosis. | objective the utility of detecting mycobacterium tuberculosis in urine samples from patients with pulmonary tuberculous with diffuse small nodular shadows (suspected miliary tuberculosis (mtb)) is still unclear in japan. a retrospective cross-sectional study was conducted to investigate the detection rates of m. tuberculosis in urine of patients with suspected mtb. methods among 687 hospitalized patients with tuberculosis, 45 with culture-confirmed suspected mtb and the data of culture and polym ... | 2017 | 28420836 |
| antibacterial, antifungal and antimycobacterial compounds from cyanobacteria. | infections from multidrug resistant (mdr) pathogenic bacteria, fungi and mycobacterium tuberculosis remain progressively intractable. the search of effective antimicrobials from other possible non-conventional sources against mdr pathogenic bacteria, fungi and mycobacteria is call of the day. this review considers 121 cyanobacterial compounds or cyano-compounds with antimicrobial activities. chemical structures of cyano-compounds were retrieved from chemspider and pubchem databases and were visu ... | 2017 | 28419973 |
| mycobacterium tuberculosis proteasomal atpase mpa has a β-grasp domain that hinders docking with the proteasome core protease. | mycobacterium tuberculosis (mtb) has a proteasome system that is essential for its ability to cause lethal infections in mice. a key component of the system is the proteasomal adenosine triphosphatase (atpase) mpa, which captures, unfolds, and translocates protein substrates into the mtb proteasome core particle for degradation. here, we report the crystal structures of near full-length hexameric mtb mpa in apo and adp-bound forms. surprisingly, the structures revealed a ubiquitin-like β-grasp d ... | 2017 | 28419599 |
| activation of tlr7 inhibition of mycobacterium tuberculosis survival by autophagy in raw 264.7 macrophages. | the aim of the study was to evaluate the effect of regulation of tlr7 on mycobacterium tuberculosis (mtb) survival in macrophages. tlr7 expression in macrophages infected by mtb was detected by rt-pcr and western blotting. regulation of tlr7 was achieved by single strand rna (ssrna) or sirna. the effects of tlr7 on mtb survival and cell viability were detected by acid fast staining and cell counting kit-8, respectively. cell ultrastructure was observed via transmission electron microscopy (tem), ... | 2017 | 28419514 |
| mutations in streptomycin resistance genes and their relationship to streptomycin resistance and lineage of mycobacterium tuberculosis thai isolates. | streptomycin (sm) is recommended by the world health organization (who) as a part of standard regimens for retreating multidrug-resistant tuberculosis (mdr-tb) cases. the incidence of mdr-tb in retreatment cases was 19% in thailand. to date, information on sm resistance (smr) gene mutations correlated to the smr of mycobacterium tuberculosis thai isolates is limited. in this study, the mutations in rpsl, rrs, gidb, and whib7 were investigated and their association to smr and the lineage of m. tu ... | 2017 | 28416956 |
| efflux attenuates the antibacterial activity of q203 in mycobacterium tuberculosis. | new and improved treatments for tuberculosis (tb) are urgently needed. recently, it has been demonstrated that verapamil, an efflux inhibitor, can reduce bacterial drug tolerance caused by efflux pump activity when administered in combination with available antituberculosis agents. the aim of this study was to evaluate the effectiveness of verapamil in combination with the antituberculosis drug candidate q203, which has recently been developed and is currently under clinical trials as a potentia ... | 2017 | 28416541 |
| inhibition of the pi3k-akt-mtor signaling pathway in t lymphocytes in patients with active tuberculosis. | to investigate pi3k-akt-mtor signaling pathway changes and the proliferation of foxp3(+)treg cells in patients with active tuberculosis. | 2017 | 28416440 |
| structural analysis of the interaction between spiroisoxazoline smart-420 and the mycobacterium tuberculosis repressor ethr2. | inhibition of transcriptional regulators of bacterial pathogens with the aim of reprogramming their metabolism to modify their antibiotic susceptibility constitutes a promising therapeutic strategy. one example is the bio-activation of the anti-tubercular pro-drug ethionamide, which activity could be enhanced by inhibiting the transcriptional repressor ethr. recently, we discovered that inhibition of a second transcriptional repressor, ethr2, leads to the awakening of a new ethionamide bio-activ ... | 2017 | 28416386 |
| smrt genome assembly corrects reference errors, resolving the genetic basis of virulence in mycobacterium tuberculosis. | the genetic basis of virulence in mycobacterium tuberculosis has been investigated through genome comparisons of virulent (h37rv) and attenuated (h37ra) sister strains. such analysis, however, relies heavily on the accuracy of the sequences. while the h37rv reference genome has had several corrections to date, that of h37ra is unmodified since its original publication. | 2017 | 28415976 |
| antitubercular activity of zno nanoparticles prepared by solution combustion synthesis using lemon juice as bio-fuel. | in this study, we report the synthesis, structural and morphological characteristics of zinc oxide (zno) nanoparticles using solution combustion synthesis method where lemon juice was used as the fuel. in vitro anti-tubercular activity of the synthesized zno nanoparticles and their biocompatibility studies, both in vitro and in vivo were carried out. the synthesized nanoparticles showed inhibition of mycobacterium tuberculosis h37ra strain at concentrations as low as 12.5μg/ml. in vitro cytotoxi ... | 2017 | 28415385 |
| fullerene-doped polyaniline as new redox nanoprobe and catalyst in electrochemical aptasensor for ultrasensitive detection of mycobacterium tuberculosis mpt64 antigen in human serum. | tuberculosis caused by mycobacterium tuberculosis (mtb) is still a major threat to global public health. however, the existing methods for mtb detection are usually complicated and time consuming with unsatisfactory sensitivity and specificity. in this work, a relatively simple and ultrasensitive electrochemical aptasensor based on novel signal generation and amplification was constructed for the determination of mtb antigen mpt64. the coil-like fullerene (c60)-doped polyaniline (c60-pan) nanohy ... | 2017 | 28414975 |
| transcriptional networks are associated with resistance to mycobacterium tuberculosis infection. | understanding mechanisms of resistance to m. tuberculosis (m.tb) infection in humans could identify novel therapeutic strategies as it has for other infectious diseases, such as hiv. | 2017 | 28414762 |
| a m. avium complex spondylodiscitis in a middle-aged woman with diabetes. | spondylodiscitis, the inflammation of the vertebral bodies and the intervertebral disk space, is the reason for low back pain in a minority of cases. this is caused by various pathogens. mycobacterium tuberculosis is responsible for 17-39% of all the cases of spondylodiscitis. on the contrast, spondylodiscitis from non tuberculous mycobacteria is extremely rare in literature. we describe a 68 -year old diabetic woman which is the first case of bone marrow involvement by m. intracellulare (member ... | 2017 | 28413773 |
| investigation of omnigene·sputum performance in delayed tuberculosis testing by smear, culture, and xpert mtb/rif assays in uganda. | omnigene·sputum (om-s) is a sample transport reagent designed to work with all tuberculosis diagnostics while eliminating the need for cold chain. om-s-treated sputum samples were assayed in several tests after multiday holds. raw sputa from 100 patients underwent direct smear microscopy, were manually split and assigned to the om-s group [om-s added at collection (no other processing required) and tested after 0- to 5-day holds at room temperature] or standard-of-care (soc) group (naoh/n-acetyl ... | 2017 | 28413105 |
| clinical research of treating tuberculous respiratory failure by sequential mechanical ventilation. | tuberculosis is kind of common chronic infectious disease, of which the pathogenic bacterium is mainly mycobacterium tuberculosis (mtb). one who is suffering severe tuberculosis may be ended with cardio-pulmonary function damage or cardio-pulmonary failure. this disease is of higher risks of complications or even high death rate, which is seriously challenging people's health and life. when a tuberculosis patient is accompanied with respiratory failure, which means the disease has developed into ... | 2016 | 28412688 |
| structural basis for ribosome protein s1 interaction with rna in trans-translation of mycobacterium tuberculosis. | ribosomal protein s1 (rpsa), the largest 30s protein in ribosome, plays a significant role in translation and trans-translation. in mycobacterium tuberculosis, the c-terminus of rpsa is known as tuberculosis drug target of pyrazinoic acid, which inhibits the interaction between mtrpsa and tmrna in trans-translation. however, the molecular mechanism underlying the interaction of mtrpsa with tmrna remains unknown. we herein analyzed the interaction of the c-terminal domain of mtrpsa with three rna ... | 2017 | 28412369 |
| the immunosuppressive effects of a novel recombinant lipq (rv2485c) protein of mycobacterium tuberculosis on human macrophage cell lines. | mycobacterium tuberculosis (mtb), an intracellular pathogen, still represents a major global health challenge. a number of mycobacterial macromolecules have been shown to target biological processes within host macrophages; however, the exact mechanism for the majority of these host pathogen interactions is still poorly understood. moreover, the lipid metabolic pathway is one of the most important physiologic pathways that plays a vital role in the survival and infection of mycobacterium tubercu ... | 2017 | 28412202 |
| qsar, docking studies of 1,3-thiazinan-3-yl isonicotinamide derivatives for antitubercular activity. | the enzyme - enoyl acyl carrier protein reductase (enoyl acp reductase) is a validated target for antitubercular activity. inhibition of this enzyme interferes with mycolic acid synthesis which is crucial for mycobacterium tuberculosis cell growth. in the present work 2d and 3d quantitative structure activity relationship (qsar) studies were carried out on a series of thiazinan-isoniazid pharmacophore to design newer analogues. for 2d qsar, the best statistical model was generated using sa-mlr m ... | 2017 | 28411471 |
| synthesis and immunogenicity of pg-tb1 monovalent glycoconjugate. | a pg-tb1 hapten from the west beijing strains of mycobacterium tuberculosis cell wall has been efficiently synthesized and conjugated to crm197 in a simple way as linker-equipped carbohydrate by applying squaric acid chemistry for an original neoglycoprotein, creating a potent t-dependent conjugate vaccine. the intermediate monoester can be easily purified and the degree of incorporation can be monitored by maldi-tof mass spectrometry. after administered systemically in mice without any adjuvant ... | 2017 | 28411454 |
| detection of pyrazinamide resistance of mycobacterium tuberculosis using nicotinamide as a surrogate. | despite the importance of pyrazinamide (pza) in tuberculosis treatment, pza susceptibility testing is not routinely performed because of its acid ph requirement. we evaluated the microplate alamar blue assay (maba) to detect resistance to pza using nicotinamide (nic) as a surrogate in neutral ph and identify the appropriate cutoff point for the assay. | 2017 | 28411185 |
| multifocal pure tubercular osteomyelitis: an unusual presentation in childhood. | tuberculosis is a major health problem in the developing world. one-third of children infected with mycobacterium tuberculosis have extra pulmonary involvement. skeletal tuberculosis occurs in 1-6% of them with vertebra being the commonest site. pure tubercular osteomyelitis without joint involvement occurs in only 2-3% cases of osteoarticular tuberculosis. common sites are femur, tibia, and fibula. disseminated skeletal involvement is very rare in children (7%) and calvarial osteomyelitis is ev ... | 2017 | 28410697 |
| pyridines: multidrug-resistant tuberculosis (mdr-tb) inhibitors. | mycobacterium tuberculosis (mtb) infection has become an increasing health threat due to the worldwide emergence of multidrug-resistant mtb (mdr-mtb) strain. isoniazid (pyridine) resistance problem is a complex process and is associated with mutations in several genes. however, the emergence of isoniazid (inh) resistant m. tuberculosis strains dictates the necessity for redesigning this old drug in order to create analogs effective against inh-resistant strains by using rational approach. in lig ... | 2017 | 28410694 |
| diallyl polysulfides from allium sativum as immunomodulators, hepatoprotectors, and antimycobacterial agents. | mycobacterium tuberculosis remains one of the world's deadliest killers, with an annual death rate of ∼1.5 million. the medicinal effects of garlic have been well documented, and natural products have been shown to have antimycobacterial activity. the current study evaluated the efficacy of six allium sativum l. polysulfide mixtures as antimycobacterial agents together with their cytotoxic, immunomodulatory, and hepatoprotective activities. the microtitre prestoblue assay was used to determine t ... | 2017 | 28410455 |
| treatment of latent tuberculosis infection. | there are approximately 56 million people who harbor mycobacterium tuberculosis that may progress to active tuberculosis (tb) at some point in their lives. modeling studies suggest that if only 8% of these individuals with latent tb infection (ltbi) were treated annually, overall global incidence would be 14-fold lower by 2050 compared to incidence in 2013, even in the absence of additional tb control measures. this highlights the importance of identifying and treating latently infected individu ... | 2017 | 28409555 |
| use of video directly observed therapy for treatment of latent tuberculosis infection - johnson county, kansas, 2015. | tuberculosis (tb) is caused by the bacterium mycobacterium tuberculosis and is spread from person to person through the air. tb can be spread in congregate settings, such as school environments, to varying degrees, based on factors including duration of contact and air ventilation (1); therefore, evaluating potential contacts and exposures can be challenging. in february 2015, a student at a kansas high school received a diagnosis of active pulmonary tb disease. screening of 385 (91%) school con ... | 2017 | 28406884 |
| increased tuberculosis patient mortality associated with mycobacterium tuberculosis mutations conferring resistance to second-line antituberculous drugs. | rapid molecular diagnostics have great potential to limit the spread of multidrug-resistant tuberculosis (mdr-tb) and extensively drug-resistant tuberculosis (xdr-tb) (m/xdr-tb). these technologies detect mutations in the mycobacterium tuberculosis genome that confer phenotypic drug resistance. however, there have been few data published regarding the relationships between the detected m. tuberculosis resistance mutations and m/xdr-tb treatment outcomes, limiting our current ability to exploit t ... | 2017 | 28404672 |
| frequency of rrs and rpsl mutations in streptomycin-resistant mycobacterium tuberculosis isolates from iranian patients. | streptomycin (sm) is one of the most effective drugs for the treatment of multidrug-resistant (mdr) tuberculosis. however, resistance to sm is increasingly reported, mainly due to mutations in the rpsl and rrs genes. this study was designed with the aim of determining the nature of sm resistance and the type and frequency of rpsl and rrs mutations among sm-resistant mycobacterium tuberculosis (mtb) isolates from iran. | 2017 | 28404234 |
| molecular detection of drug resistance to ofloxacin and kanamycin in mycobacterium tuberculosis by using multiplex allele-specific pcr. | drug resistance in tuberculosis (tb) is the biggest global health challenge as it hinders the tuberculosis control program and makes the disease more worsen. molecular methods interrupt the spread of drug resistance by facilitating the appropriate anti- tuberculosis therapy at correct time through rapid diagnosis of multi drug resistant (mdr) and extensively drug resistant tuberculosis (xdr-tb). in this study we standardized and evaluated the diagnostic utility of multiplex allele specific pcr ( ... | 2017 | 28404233 |
| tuberculosis among prison staff in rio grande do sul. | to evaluate the risk of infection and illness caused by mycobacterium tuberculosis among health care and security staff in prisons in two regions of rio grande do sul (rs). | 2017 | 28403282 |
| association of vitamin d deficiency, season of the year, and latent tuberculosis infection among household contacts. | vitamin d (vd) enhances the immune response against mycobacterium tuberculosis in vitro, and vd deficiency has been described in patients with active tuberculosis (tb). however, the role of hypovitaminosis d in the pathogenesis of early tb infection acquisition is unclear. we aimed to evaluate the association of vd deficiency, season of the year, and latent tb infection in household contacts (hhc), given that this is a potentially modifiable condition often related to nutritional deficiencies an ... | 2017 | 28403225 |
| the risk of mycobacterium tuberculosis transmission from pediatric index cases to school pupils. | this study is the largest report on mycobacterium tuberculosis transmission rate (tr) from children with pulmonary tuberculosis to school pupils. higher tr (around 21.6%) was observed in contacts of smear-positive children. tr from pediatric smear-negative index cases was around 0. if our data are confirmed, school contacts of a smear-negative index case could be screened only by clinical evaluation and tuberculin skin test, avoiding the routine use of chest radiographs in children less than 5 y ... | 2017 | 28403060 |
| bcl-xl mediates ripk3-dependent necrosis in m. tuberculosis-infected macrophages. | virulent mycobacterium tuberculosis (mtb) triggers necrosis in host mϕ, which is essential for successful pathogenesis in tuberculosis. here we demonstrate that necrosis of mtb-infected mϕ is dependent on the action of the cytosolic receptor interacting protein kinase 3 (ripk3) and the mitochondrial bcl-2 family member protein b-cell lymphoma-extra large (bcl-xl). ripk3-deficient mϕ are able to better control bacterial growth in vitro and in vivo. mechanistically, cytosolic ripk3 translocates to ... | 2017 | 28401933 |
| tuberculosis in children with sickle cell anaemia: a retrospective study in french tertiary care centres. | tuberculosis (tb) and sickle cell anaemia (sca) may affect the same population of patients, particularly in africa but also in high-tb incidence areas in developed countries. however, few data are available from children with sca who develop tb. the aim of this study was to describe the clinical features and outcome of tb diagnosed in children with sca. we conducted a retrospective, descriptive study in three referral centre of sickle cell disease in paris, france. we included 11 patients with s ... | 2017 | 28401342 |
| nontuberculous mycobacterial infection of the musculoskeletal system in immunocompetent hosts. | nontuberculous mycobacteria (ntm) were considered saprophytic organisms for many years but now are recognized as human pathogens. although humans are routinely exposed to ntm, the rate of clinical infection is low. such infections usually occur in the elderly and in patients who are immunocompromised. however, there has been an increasing incidence in recent years of infections in immunocompetent hosts. ntm infections in immunocompetent individuals are secondary to direct inoculation either cont ... | 2017 | 28400668 |
| targeting energy metabolism in mycobacterium tuberculosis, a new paradigm in antimycobacterial drug discovery. | drug-resistant mycobacterial infections are a serious global health challenge, leading to high mortality and socioeconomic burdens in developing countries worldwide. new innovative approaches, from identification of new targets to discovery of novel chemical scaffolds, are urgently needed. recently, energy metabolism in mycobacteria, in particular the oxidative phosphorylation pathway, has emerged as an object of intense microbiological investigation and as a novel target pathway in drug discove ... | 2017 | 28400527 |
| type-1 polarised dendritic cells are a potent immunogen against mycobacterium tuberculosis. | application of immunotherapy using dendritic cells (dcs) is considered an effective treatment strategy against persistent mycobacterium tuberculosis infection. with the goal of developing improved therapeutic vaccination strategies for patients with tuberculosis (tb), we tested the ability of ex vivo-generated dcs to induce an effective tb antigen-specific type-1 immune response. | 2017 | 28399967 |
| impact of euro-american sublineages of mycobacterium tuberculosis on new infections among named contacts. | the impact of demographic, clinical, and bacterial factors on new infection by euro-american lineage mycobacterium tuberculosis among contacts of patients with tuberculosis (tb) has not been evaluated. | 2017 | 28399965 |
| pooling sputum samples to improve the feasibility of xpert(®) mtb/rif in systematic screening for tuberculosis. | systematic screening for tuberculosis (tb) using xpert® mtb/rif. | 2017 | 28399964 |
| tuberculosis of the glans penis: an important differential diagnosis of genital ulcer disease. | we report a 45-year-old, apparently healthy sero-negative man, presenting with multiple ulcers on the glans penis for a duration of three months. there was no significant inguinal lymphadenopathy. he showed no improvement on systemic antibiotics and acyclovir. histopathology revealed the diagnosis of genital tuberculosis (tb), and polymerase chain reaction for mycobacterium tuberculosis tested positive. the patient responded well to category i anti-tubercular treatment with complete resolution o ... | 2017 | 28399708 |
| nicotine impairs macrophage control of mycobacterium tuberculosis. | pure nicotine impairs macrophage killing of mycobacterium tuberculosis (mtb) but it is not known whether the nicotine component in cigarette smoke (cs) plays a role. moreover, the mechanisms by which nicotine impairs macrophage immunity against mtb have not been explored. to neutralize the effects of nicotine in cs extract, we utilized a competitive inhibitor to the nicotinic acetylcholine receptor (nachr) - mecamylamine - as well as macrophages derived from mice with genetic disruption of speci ... | 2017 | 28398760 |
| drug resistance in newly presenting and previously treated tuberculosis patients in guangxi province, people's republic of china. | drug-resistant mycobacterium tuberculosis strains are a major threat to the control of tuberculosis (tb), but the prevalence of drug-resistant tb is still unknown in the southern ethnic region of china. a cluster-randomized sampling method was used to include the study population. isolates were tested for resistance to 6 antituberculosis drugs, and genotyped to identify beijing strains. overall, 11.3% (139/1229) of new cases and 33.0% (126/382) of retreated cases had drug-resistant tuberculosis. ... | 2017 | 28397531 |
| the activity of a hexameric m17 metallo-aminopeptidase is associated with survival of mycobacterium tuberculosis. | mycobacterium tuberculosis is one of the most prevalent human pathogens causing millions of deaths in the last years. moreover, tuberculosis (tb) treatment has become increasingly challenging owing to the emergence of multidrug resistant m. tuberculosis strains. thus, there is an immediate need for the development of new anti-tb drugs. proteases appear to be a promising approach and may lead to shortened and effective treatments for drug-resistant tb. although the m. tuberculosis genome predicts ... | 2017 | 28396657 |
| enhanced respiration prevents drug tolerance and drug resistance in mycobacterium tuberculosis. | persistence, manifested as drug tolerance, represents a significant obstacle to global tuberculosis control. the bactericidal drugs isoniazid and rifampicin kill greater than 99% of exponentially growing mycobacterium tuberculosis (mtb) cells, but the remaining cells are persisters, cells with decreased metabolic rate, refractory to killing by these drugs, and able to generate drug-resistant mutants. we discovered that the combination of cysteine or other small thiols with either isoniazid or ri ... | 2017 | 28396391 |
| [application of the bacspreader™ microbe dispersion counter in drug susceptibility test on mycobacterium tuberculosis]. | objective: to evaluate the application of the bacspreader™ microbe dispersion counter in drug susceptibility test (dst) on mycobacterium tuberculosis (mtb). methods: the mtb strains were dispersed and diluted to 1.0 mcfarland standard turbidity, by means of bacspreader™ microbe dispersion counter and manual grinding method, respectively. the bacterial dispersion effect and bacterial activity were tested by microscope and colony counting method. during jan. 2015 to june 2015, a total of 726 isola ... | 2017 | 28395409 |
| an opposing view on including high school students in a latent tuberculosis infection control program in korea. | the korea centers for diseases control and prevention (kcdc) has announced a control program against latent mycobacterium tuberculosis infection (ltbi), for a "tb-safe country" this year with the goal of a "tb-free korea" by 2025. the program includes high school students as one target group; however, some school health teachers and parents have expressed their opposition to this. the 2015 world health organization guidelines do not recommend inclusion of asymptomatic high school students in ltb ... | 2017 | 28395400 |
| genomic analyses of the ancestral manila family of mycobacterium tuberculosis. | with its airborne transmission and prolonged latency period, mycobacterium tuberculosis spreads worldwide as one of the most successful bacterial pathogens and continues to kill millions of people every year. m. tuberculosis lineage 1 is inferred to originate ancestrally based on the presence of the 52-bp tbd1 sequence and analysis of single nucleotide polymorphisms. previously, we briefly reported the complete genome sequencing of m. tuberculosis strains 96121 and 96075, which belong to the anc ... | 2017 | 28394899 |
| 18f-fdg pet/ct of tuberculosis meningitis and carotid pseudoaneurysm. | a 26-year-old woman presented with a headache, left-sided weakness of the body, and pulsatile swelling above the sternal notch. she was treated for abdominal tuberculosis in the past, two years later she developed miliary tuberculosis and was put on modified regimen of antitubercular drugs in view of drug induced hepatitis. ultrasound neck revealed saccular aneurysm measuring 4 x 3 cm, located in between common carotid arteries with an eccentric thrombus. mri brain revealed multiple tuberculoma ... | 2017 | 28394836 |
| structure of the mycobacterial esx-5 type vii secretion system membrane complex by single-particle analysis. | mycobacteria are characterized by their impermeable outer membrane, which is rich in mycolic acids(1). to transport substrates across this complex cell envelope, mycobacteria rely on type vii (also known as esx) secretion systems(2). in mycobacterium tuberculosis, these esx systems are essential for growth and full virulence and therefore represent an attractive target for anti-tuberculosis drugs(3). however, the molecular details underlying type vii secretion are largely unknown, due to a lack ... | 2017 | 28394313 |
| association of c-type lectin mincle with fcεriβγ subunits leads to functional activation of rbl-2h3 cells through syk. | macrophage-inducible c-type lectin (mincle) interacts with the γ-subunit of high-affinity ige receptor (fcεriγ) and activates syk by recognizing its specific ligand, trehalose-6,6'-dimycolate, a glycolipid produced by mycobacterium tuberculosis. it has been suggested that mast cells participate in the immune defense against pathogenic microbes including m. tuberculosis, although the functions are still uncertain. in this study, we examined the mincle-mediated signaling pathway and cellular respo ... | 2017 | 28393919 |
| improving basic and membrane protein ms detection of the culture filtrate proteins from mycobacterium tuberculosis h37rv by biomimetic affinity pre-fractionation. | the culture filtrate proteins (cfps) from mycobacterium tuberculosis (mtb) have been shown to induce protective immune responses in human and animal models, making them a promising source of candidate targets for tuberculosis drugs, vaccines and diagnostics. the constituents of the mtb cfp proteome are complex and vary with growth conditions. to effectively profile cfps, gel-based pre-fractionation is usually performed before ms analysis. in this study, we describe a novel pre-fractionation appr ... | 2017 | 28393466 |
| early diagnosis of tuberculosis-associated iga nephropathy with esat-6. | iga nephropathy (igan) is the most common cause of primary renal diseases worldwide, and the early secreted antigenic target of 6 (esat-6) which was secreted by mycobacterium tuberculosis (mtb) may be involved in the development and progression of igan. this study aimed to investigate the role of esat-6 for early diagnosis of igan caused by mtb infection. from 2011 to 2014, 21 patients with renal tuberculosis (rtb), 25 with igan, and 46 with igan infected with mtb (igan/mtb) were enrolled. serum ... | 2017 | 28392505 |
| anti-inflammatory, antimycobacterial and genotoxic evaluation of doliocarpus dentatus. | doliocarpus dentatus is a medicinal plant widely used in mato grosso do sul state for removing the swelling pain caused by the inflammation process and for treating urine retention. | 2017 | 28392458 |
| prevalence of latent mycobacterium tuberculosis infection (ltbi) in saudi arabia; population based survey. | the annual risk of tuberculosis infection (arti) data in saudi arabia has not been updated since 1993. | 2017 | 28392316 |
| hybrid mass spectrometry approaches to determine how l-histidine feedback regulates the enzyzme mtatp-phosphoribosyltransferase. | mtatp-phosphoribosyltransferase (mtatp-prt) is an enzyme catalyzing the first step of the biosynthesis of l-histidine in mycobacterium tuberculosis, and proposed to be regulated via an allosteric mechanism. native mass spectrometry (ms) reveals mtatp-prt to exist as a hexamer. conformational changes induced by l-histidine binding and the influence of buffer ph are determined with ion mobility ms, hydrogen deuterium exchange (hdx) ms, and analytical ultracentrifugation. the experimental collision ... | 2017 | 28392260 |
| structural basis of mycobacterium tuberculosis transcription and transcription inhibition. | mycobacterium tuberculosis (mtb) is the causative agent of tuberculosis, which kills 1.8 million annually. mtb rna polymerase (rnap) is the target of the first-line antituberculosis drug rifampin (rif). we report crystal structures of mtb rnap, alone and in complex with rif, at 3.8-4.4 å resolution. the results identify an mtb-specific structural module of mtb rnap and establish that rif functions by a steric-occlusion mechanism that prevents extension of rna. we also report non-rif-related comp ... | 2017 | 28392175 |
| lung tissue concentrations of pyrazinamide among patients with drug-resistant pulmonary tuberculosis. | improved knowledge regarding the tissue penetration of antituberculosis drugs may help optimize drug management. patients with drug-resistant pulmonary tuberculosis undergoing adjunctive surgery were enrolled. serial serum samples were collected, and microdialysis was performed using ex vivo lung tissue to measure pyrazinamide concentrations. among 10 patients, the median pyrazinamide dose was 24.7 mg/kg of body weight. imaging revealed predominant lung lesions as cavitary (n = 6 patients), mass ... | 2017 | 28373198 |
| stratification of latent mycobacterium tuberculosis infection by cellular immune profiling. | recently acquired and remotely acquired latent mycobacterium tuberculosis infection (ltbi) are clinically indistinguishable, yet recent acquisition of infection is the greatest risk factor for progression to tuberculosis in immunocompetent individuals. we aimed to evaluate the ability of cellular immune signatures that differ between active tuberculosis and ltbi to distinguish recently from remotely acquired ltbi. | 2017 | 28329119 |
| evaluation of quantiferon-tb gold-plus in health care workers in a low-incidence setting. | although launched in 2015, little is known about the accuracy of quantiferon-tb gold-plus (qft-plus) for diagnosis of latent m. tuberculosis infection (ltbi). unlike its predecessor, qft-plus utilizes two antigen tubes to elicit an immune response from cd4(+) and cd8(+) t lymphocytes. we conducted a cross-sectional study in low-risk health care workers (hcws) at a single u.s. center to compare qft-plus to quantiferon-tb gold in-tube (qft). a total of 989 hcws were tested with both qft and qft-pl ... | 2017 | 28298455 |
| concentration-dependent antagonism and culture conversion in pulmonary tuberculosis. | there is scant evidence to support target drug exposures for optimal tuberculosis outcomes. we therefore assessed whether pharmacokinetic/pharmacodynamic (pk/pd) parameters could predict 2-month culture conversion. | 2017 | 28205671 |
| oxidative stress and tb outcomes in patients with diabetes mellitus? | in patients with diabetes mellitus, tb treatment outcomes are poorer. most parameters, when measured, reflect the slower bacteriological conversion from positivity to negativity and higher risks of disease relapse and mortality, as well as a greater propensity to develop drug-resistant tb. aside from the well-known immunological dysfunction inherent to patients with diabetes mellitus, oxidative stress is likely a major underlying mechanism adversely impacting their tb treatment outcomes. mycobac ... | 2017 | 28204508 |
| interaction of rifampin and darunavir-ritonavir or darunavir-cobicistat in vitro. | treatment of hiv-infected patients coinfected with mycobacterium tuberculosis is challenging due to drug-drug interactions (ddis) between antiretrovirals (arvs) and antituberculosis (anti-tb) drugs. the aim of this study was to quantify the effect of cobicistat (cobi) or ritonavir (rtv) in modulating ddis between darunavir (drv) and rifampin (rif) in a human hepatocyte-based in vitro model. human primary hepatocyte cultures were incubated with rif alone or in combination with either cobi or rtv ... | 2017 | 28193650 |
| clinical impact and cost-effectiveness of xpert mtb/rif testing in hospitalized patients with presumptive pulmonary tuberculosis in the united states. | microscopic examination of acid-fast-stained sputum smears is the current standard of care in the united states to determine airborne infection isolation (aii) of inpatients with presumptive pulmonary tuberculosis (ptb). however, nucleic acid amplification testing (naat) with the xpert mtb/rif assay (xpert) may be more efficient and less costly. | 2017 | 28172666 |
| profile of tuberculous cervical lymphadenopathy in children. | analyze clinical profile of tuberculous cervical lymphadenopathy in children. | 2017 | 28158382 |
| effect of pregnancy on interferon gamma release assay and tuberculin skin test detection of latent tb infection among hiv-infected women in a high burden setting. | peripartum immunologic changes may affect latent tuberculosis infection (ltbi) diagnostic performance among hiv-infected women. | 2017 | 28141782 |
| tackling drug-resistant tuberculosis: current trends and approaches. | tuberculosis is very much rampant in our society and accounts for a large number of deaths annually. in spite of consistent efforts being made, the disease has not been curtailed yet. the emergence of mdr and xdr strains in the society along with an increase in the number of hiv cases and that of latent tb, have further aggravated the problem making the disease very much persistent. the current situation clearly manifests the need to discover and develop new potent molecules/approaches that coul ... | 2017 | 27280980 |
| drug resistance profiles of new- and previously treated patients with pulmonary tuberculosis in ibadan, nigeria. | information on tb drug resistance profiles and its' associated risk factors are scarce in nigeria despite the large burden of disease in the country. the study was designed to report drug resistance profiles of new- and previously treated patients with pulmonary tuberculosis (tb) in ibadan, nigeria. | 2016 | 28686829 |
| collision of three pandemics: the coexistence of cervical cancer, hiv infection, and prior tuberculosis in the sub-saharan country of botswana. | cervical cancer is the leading cause of cancer-related mortality in the developing world, where hiv and mycobacterium tuberculosis (tb) infection are also endemic. hiv infection is independently associated with increased morbidity and mortality among women with cervical cancer. tb is believed to increase the risk of malignancies and could cause chronic inflammation in the gynecologic tract. however, the relationship between cervical cancer and tb in settings hyperendemic for hiv is unknown. we f ... | 2016 | 28717682 |
| a genomics-led approach to deciphering the mechanism of thiotetronate antibiotic biosynthesis. | thiolactomycin (tlm) is a thiotetronate antibiotic that selectively targets bacterial fatty acid biosynthesis through inhibition of the β-ketoacyl-acyl carrier protein synthases (kasi/ii) that catalyse chain elongation on the type ii (dissociated) fatty acid synthase. it has proved effective in in vivo infection models of mycobacterium tuberculosis and continues to attract interest as a template for drug discovery. we have used a comparative genomics approach to uncover the (hitherto elusive) bi ... | 2016 | 28791099 |
| [tuberculosis in general practice in belgium : what's new?] | tuberculosis is still an important disease in belgium, mainly in big agglomerations. for this reason, knowledge of this entity by a general practitioner is very important. genetic tests able to identify the species of mycobacterium tuberculosis have been developed in recent years, but also identification of resistance to 1st and 2nd line treatment through genetic methods has been developed. the quick availability of results of this tests compared to the standard tests is a big advantage. treatme ... | 2016 | 28525233 |
| surface expression and genetic variants of toll-like receptor 2 in pulmonary tuberculosis patients. | innate immunity plays an important role in the host defense against mycobacterium tuberculosis (m. tuberculosis). toll-like receptor-2 (tlr2) is the main receptor and one of central components in initiation of innate immunity against m. tuberculosis. the study aimed to evaluate tlr2 surface expression on peripheral blood monocytes and study its association with variants in tlr2 597t/c single nucleotide polymorphisms (snps) in patients with pulmonary tuberculosis. fifty pulmonary tuberculosis pat ... | 2016 | 28502147 |
| eosinophilic cystitis and cholangitis - systemic disease triggered by mycobacterium tuberculosis? | eosinophilic cystitis (ec) is a rare inflammatory disorder of the urinary tract characterized by infiltration of bladder with eosinophils. the cause remains unclear, immunological mechanisms have been implicated in pathogenesis. potential etiological factors include: tumors, allergy, parasitic infections, trauma. the disease may have a variable course, from a mild self-limiting, through common symptoms like: dysuria, hematuria, abdominal pain, tumor, to severe renal failure, with eosinophilic in ... | 2016 | 28478431 |
| prevalence of multidrug-resistant tuberculosis cases among hiv-positive and hiv-negative patients eligible for retreatment regimen in togo using genexpert mtb/rif. | drug-resistant tuberculosis (tb) is emerging as an important health problem in togo. from sputum of previously treated tb patients, multidrug-resistant (mdr) tb was diagnosed in 24% (10/42) patients via genexpert mtb/rif compared to 25% (6/24) patients via conventional drug susceptibility testing (bactec mgit 960 system). the agreement between these two methods to detect mdr-tb is excellent. however, genexpert mtb/rif offers the advantage of rapidly detecting mycobacterium tuberculosis complex i ... | 2015 | 28626586 |
| [etiology of bacterial infections and incidence of comorbidities in patients with tuberculosis, treated in mazovian treatment centre of tuberculosis and lung diseases during years 2012-2014]. | tuberculosis (tb) is an infectious disease caused by the mycobacterium tuberculosis. infection occurs mostly via inhalation, while the immune system is weakened. tb can take a pulmonary or extrapulmonary form. treatment involves an intensive, long-term antimycobacterial multidrug therapy. tb cases are recorded on the worldwide scale. the morbidity in poland varies territorially. | 2015 | 28501834 |
| vitamin d, serum 25(oh)d, ll-37 and polymorphisms in a canadian first nation population with endemic tuberculosis. | background canadian first nation populations have experienced endemic and epidemic tuberculosis (tb) for decades. vitamin d-mediated induction of the host defence peptide ll-37 is known to enhance control of pathogens such as mycobacterium tuberculosis. objective evaluate associations between serum levels of 25-hydroxy vitamin d (25(oh)d) and ll-37, in adult dene first nation participants (n = 34) and assess correlations with single nucleotide polymorphisms (snps) in the vitamin d receptor (vdr) ... | 2015 | 28417792 |
| worms and humans: a happy divorce? | chronic asymptomatic worm infection, often in combination with tuberculosis (tb), is common in low-income countries. indeed, a life without worm infestation, as is now the case in most high-income countries, is a recent condition for humankind. worms and mycobacterium tuberculosis give rise to different immune response patterns (th2 vs. th1 driven), and we have studied whether chronic worm infection affects the susceptibility to and control of tb in the low income country of ethiopia. our result ... | 2015 | 28392966 |
| a case of disseminated tuberculosis after the initiation of hemodialysis. | a 67-year-old man with diabetes mellitus, chronic kidney disease, chronic heart failure, and amputation of the left lower limb was admitted to our hospital with decreasing renal function. on admission, he was started on hemodialysis. he simultaneously developed a urinary tract infection, infected necrotic wound of the right lower limb, and pneumonia, and was treated with broad-spectrum antibiotics. although his general condition improved, fever and anorexia persisted and jaundice worsened. he di ... | 2013 | 28509302 |
| interferon γ-release assays for detecting latent tuberculosis infection in patients scheduled for anti-tnfα therapy. | screening for, and treatment of, latent tuberculosis infection (ltbi) before anti- tumour necrosis factor α therapy has been shown to decrease the incidence of active tuberculosis by more than 80% and is recommended before initiation of treatment. in the absence of a 'gold standard' test for ltbi, current screening involves taking a clinical history of risk factors, chest radiograph and tuberculin skin test. alternative cellular immune-based screening tests have been developed to detect mycobact ... | 2011 | 28839578 |
| recent advances in tuberculosis diagnostic techniques. | tuberculosis is re-emerging as an important cause of morbidity and mortality in man. this article outlines current strategies available for the diagnosis of tuberculosis, and its applicability. fluorescent staming, modified culture methods, antigen detection, elisa based assays against various antigen preparation and recent advances in molecular techniques have been outlined. present strategies being developed at armed forces medical college for the early diagnosis, speciation, antibiotic sensit ... | 2000 | 28790679 |
| multidrug resistant tuberculosis - biomechanism, epidemiology and management strategies. | muitidrug resistant tuberculosis has shown an alarming increase and this assumes added importance in view of the increasing number of hiv infected patients. this article reviews the biomechanism of resistance and discusses the present stategies that are available and recommended to tackle the rising incidence of tuberculosis due to resistant mycobacteria. | 1997 | 28769486 |
| the role of rapid slide culture in the diagnosis and management of pulmonary tuberculosis. | rapid slide culture method using human blood medium was utilized for the primary culture of mycobacterium tuberculosis and the results obtained were compared with results of smear examination of sputum specimens from fresh cases of pulmonary tuberculosis. smear and rapid slide culture results of a total of 320 patients were analyzed. slide culture was positive in 104 cases whereas smears were positive in 90 cases. early culture confirmation in 7 days coupled with positivity better than smear exa ... | 1995 | 28769283 |
| storage and retrieval of mycobacterium tuberculosis on filter paper strips. | | 1994 | 28769224 |