| two cysteine protease inhibitors, ehicp1 and 2, localized in distinct compartments, negatively regulate secretion in entamoeba histolytica. | the enteric protozoan parasite entamoeba histolytica uniquely possesses two isotypes of icps, a novel class of inhibitors for cysteine proteases. these two ehicps showed a remarkable difference in the ability to inhibit cysteine protease (cp) 5, a well-established virulence determinant, whereas they equally inhibited cp1 and cp2. immunofluorescence imaging and cellular fractionation showed that ehicp1 and ehicp2 are localized to distinct compartments. while ehicp1 is localized to the soluble cyt ... | 2006 | 16979632 |
| genetic diversity of glucose phosphate isomerase from entamoeba histolytica. | to investigate the molecular basis of zymodeme analysis in the enteric protozoan parasite entamoeba histolytica, genes encoding glucose phosphate isomerase (gpi) were isolated from four representative e. histolytica strains belonging to zymodeme ii, iialpha-, xiv, or xix. two alleles were obtained from each strain; six alleles with eight polymorphic nucleotide positions were identified among the four strains. two of these eight polymorphic nucleotides resulted in non-conserved amino acid substit ... | 2006 | 16979933 |
| structural basis for the observed differential magnetic anisotropic tensorial values in calcium binding proteins. | lanthanide ions (ln(3+)), which have ionic radii similar to those of ca(2+), can displace the latter in a calcium binding protein, without affecting its tertiary structure. the paramagnetic ln(3+) possesses large anisotropic magnetic susceptibilities and produce pseudocontact shifts (pcss), which have r(-3) dependence. the pcs can be seen for spins as far as 45 a from the paramagnetic ion. they aid in structure refinement of proteins by providing long-range distance constraints. besides, they ca ... | 2006 | 16981203 |
| amoebic hepatic abscesses in an hiv-positive patient. | herein we report the case of hepatic amoebic abscesses in an hiv-positive italian seaman with a history of promiscuous heterosexual intercourse. in october 2004, the patient was hospitalized because of fever and recurring abdominal pain. abdominal ultrasonography revealed six hepatic hypoechoid oval lesions with hyperechoid margins. stool samples were negative for parasites and bacteria, and serology for entamoeba histolytica was also negative. therapy with meropenem plus levofloxacin was initia ... | 2006 | 16987046 |
| a phagocytosis mutant of entamoeba histolytica is less virulent due to deficient proteinase expression and release. | cysteine proteinases are key virulence factors of entamoeba histolytica that are released during the process of invasion. we used a chemical mutant of e. histolytica strain hm-1:imss, clone l6, which is deficient in virulence, phagocytosis, and cysteine proteinase activity to help define the mechanisms of cysteine proteinase release. all cysteine proteinase genes of wild type hm-1 were present in the l6 mutant genome, but three of the major expressed proteinases, ehcp1, ehcp2, and ehcp5 were bot ... | 2007 | 16987516 |
| toward a rational design of selective multi-trypanosomatid inhibitors: a computational docking study. | compound v7, a benzothiazole which was recently found as selective inhibitor of trypanosomal tims, was docked into tims from trypanosoma cruzi, trypanosoma brucei, entamoeba histolytica, plasmodium falciparum, yeast, and human. structural analyses revealed the importance of the accessibility to the two aromatic clusters located at the dimer's interface for the selective inhibition of trypanosomal tims. thus, it was found that different accessibilities of the protein interface of tims plays an im ... | 2006 | 16997551 |
| aberrant induction of regulatory activity of cd4+cd25+ t cells by dendritic cells in hiv-infected persons with amebic liver abscess. | to know why hiv-1-infected persons are particularly susceptible to amebic liver abscess (ala), we investigated the role of cd4cd25 t cells in the susceptibility of hiv-1-infected persons to this disease. herein we show, in early stage hiv-1-infected subjects, that cd4 t-cell responses to entamoeba histolytica antigen (ehag) were selectively impaired, especially in those with ala. ehag-specific cd4 t-cell responses were normalized by depletion of cd4cd25 cells or by addition of anti-cytotoxic t l ... | 2007 | 17003690 |
| expression, purification and crystallization of l-methionine gamma-lyase 2 from entamoeba histolytica. | l-methionine gamma-lyase (mgl) is considered to be an attractive target for rational drug development because the enzyme is absent in mammalian hosts. to enable structure-based design of drugs targeting mgl, one of the two mgl isoenzymes (ehmgl2) was crystallized in the orthorhombic space group p2(1)2(1)2(1), with unit-cell parameters a = 88.89, b = 102.68, c = 169.87 a. the crystal diffracted to a resolution of 2.0 a. the presence of a tetramer in the asymmetric unit (4 x 43.1 kda) gives a matt ... | 2006 | 17012806 |
| multidrug resistance in amoebiasis patients. | amoebiasis, caused by entamoeba sp. a protozoan parasite, is a major public health problem in tropical and subtropical countries. the symptomatic patients are treated by specific chemotherapy. however, there are reports of treatment failure in some cases suggesting the possibility of drug resistance. the present study was therefore planned to assess the presence and expression of mrna of multidrug resistance (mdr) gene in clinical isolates of entamoeba histolytica and e. dispar. | 2006 | 17015933 |
| protozoan parasites that infect the gastrointestinal tract. | protozoa that infect the gastrointestinal tract include the deadly parasite entamoeba histolytica;giardia lamblia, the most common cause of waterborne disease outbreaks; and the large group of spore-forming parasites that share a green algae symbiont and a predilection for causing chronic diarrhea in immunocompromised persons. some recent advances in the epidemiology, pathogenesis, diagnosis, and treatment of the diseases caused by these protozoa are discussed in this review. | 2000 | 17024011 |
| emerging and reemerging intestinal protozoa. | the intestinal protozoa have gained importance to physicians practicing medicine in the united states, canada, and europe during recent years as a result of increasing world travel, the globalization of the world's economy, and the growing number of chronically immunosuppressed people. during the spring of 1996, cyclospora cayetanensis caused diarrhea in approximately 1500 people exposed to guatemalan raspberries. this epidemic recurred in 1997, emphasizing the risks of the global economy and fo ... | 2001 | 17031144 |
| proteomic analysis of entamoeba histolytica. | in this study, the proteome of axenically grown entamoeba histolytica parasites was explored by two-dimensional gel electrophoresis (2-de), employing a practical and effective procedure for the solubilization of e. histolytica proteins. approximately 900 protein species in the ph range between 4 and 7 were detected by coomassie blue staining. ninety-five spots were excised, trypsinated and subjected to mass spectrometry. the resultant data from peptide mass fingerprints were compared with those ... | 2007 | 17032470 |
| parasitic gut infections. | emerging spore-forming protozoa such as cryptosporidia and microsporidia are becoming major public health problems in developing countries as well as in the developed world. they are a new addition to an already long list of intestinal parasites, but their diagnosis is much more difficult than for well-known protozoa and helminths. fortunately, enzyme immunoassay and polymerase chain reaction techniques are becoming available not only for the detection of cryptosporidium parvum and the microspor ... | 1998 | 17033427 |
| evaluation of entamoeba histolytica antigen and antibody point-of-care tests for the rapid diagnosis of amebiasis. | the bedside diagnosis of amebiasis could improve patient care. in bangladesh and vietnam, a novel and simple-to-use entamoeba histolytica rapid antigen test had 97% sensitivity and 100% specificity compared to the results of a standard enzyme-linked immunosorbent assay antigen detection method, and a rapid antibody test had 89 to 100% sensitivity and 89 to 95% specificity. | 2006 | 17035498 |
| preliminary evidence on existence of transplasma membrane electron transport in entamoeba histolytica trophozoites: a key mechanism for maintaining optimal redox balance. | entamoeba histolytica, an amitochondriate parasitic protist, was demonstrated to be capable of reducing the oxidized form of alpha-lipoic acid, a non permeable electron acceptor outside the plasma membrane. this transmembrane reduction of non permeable electron acceptors with redox potentials ranging from -290 mv to +360 mv takes place at neutral ph. the transmembrane reduction of non permeable electron acceptors was not inhibited by mitochondrial electron transport inhibitors such as antimycin ... | 2006 | 17039394 |
| identification of insertion hot spots for non-ltr retrotransposons: computational and biochemical application to entamoeba histolytica. | the genome of the human pathogen entamoeba histolytica contains non-long terminal repeat (ltr) retrotransposons, the ehlines and ehsines, which lack targeted insertion. we investigated the importance of local dna structure, and sequence preference of the element-encoded endonuclease (en) in selecting target sites for retrotransposon insertion. pre-insertion loci were tested computationally to detect unique features based on dna structure, thermodynamic considerations and protein interaction meas ... | 2006 | 17040894 |
| use of molecular tools to distinguish entamoeba histolytica and entamoeba dispar infection among the aborigines in cameron highlands. | amoebiasis is an infectious diseased caused by parasitic one-celled protozoan called entamoeba histolytica. numerous protozoa also can inhabit the gastro-intestinal tract of human. majority of these protozoa are non-pathogenic commensals or only causes disease under certain circumstances. morphologically, e. histolytica, the invasive form, share the same characteristic with the nonpathogenic form, e. dispar. both strains can be distinguished by using dna identification. many previous researches ... | 2006 | 17041549 |
| some epidemiological aspects of intestinal parasites in women workers before going abroad. | a study was conducted in jakarta on 903 women workers before going abroad through stool examination by ritchie's technical method. of the women workers studied, 640 subjects (70.87%) were found to be infected with intestinal parasites either helminthes, protozoa or combination. out of those infected, 451 (70.47%) subjects were infected with intestinal helminthes, namely ascaris lumbricoides (38.13%), trichuris trichiura (28.13%), a combination of necator americanus and ancylostoma duodenale (13. ... | 2006 | 17041558 |
| infection of blastocystis hominis in primary schoolchildren from nakhon pathom province, thailand. | a study was conducted to evaluate the infection status of blastocystis hominis in children from four public schools in phuttamonthon district, nakhon pathom province, thailand during november to december 2004. a total of 814 faecal specimens were used for b. hominis cultivation using jones' medium. mixed infections with other intestinal parasites were also examined by formalin ethyl acetate concentration method. it was found that 13.51% (110 of 814) of the children examined were infected with b. ... | 2006 | 17041560 |
| ehrho1, a rhoa-like gtpase of entamoeba histolytica, is modified by clostridial glucosylating cytotoxins. | clostridial glucosylating cytotoxins inactivate mammalian rho gtpases by mono-o glucosylation of a conserved threonine residue located in the switch 1 region of the target protein. here we report that ehrho1, a rhoa-like gtpase from the protozoan parasite entamoeba histolytica, is glucosylated by clostridial cytotoxins. recombinant glutathione s-transferase-ehrho1 and ehrho1 from cell lysate of entamoeba histolytica were glucosylated by clostridium difficile toxin b and clostridium novyi alpha-t ... | 2006 | 17056697 |
| differentiation of entamoeba histolytica from entamoeba dispar by pcr-coupled nonisotopic sscp analysis. | entamoeba histolytica is a pathogenic protozoan parasite, which causes amoebic colitis, dysentery and liver abscesses in humans. since the cyst and small trophozoite stages of this parasite are indistinguishable by light microscopy from entamoeba dispar (which is nonpathogenic), specific diagnosis is compromised. to overcome this limitation, a pcr-coupled sscp approach, utilising a sequence difference of 4.6% in a short region ( approximately 173-174 bp) of the small subunit of nuclear ribosomal ... | 2006 | 17058306 |
| sensing dna methylation in the protozoan parasite entamoeba histolytica. | in the protozoan parasite entamoeba histolytica, 5-methylcytosine (m5c) was found predominantly in repetitive elements. its formation is catalysed by ehmeth, a dna methyltransferase that belongs to the dnmt2 subfamily. here we describe a 32 kda nuclear protein that binds in vitro with higher affinity to the methylated form of a dna encoding a reverse transcriptase of an autonomous non-long-terminal repeat retrotransposon (rt line) compared with the non-methylated rt line. this protein, named e. ... | 2006 | 17059565 |
| clinical significance of the redefinition of the agent of amoebiasis. | entamoeba histolytica is the pathogenic species of entamoeba that causes amoebic dysentery and other invasive disease. the morphologically similar species, e. dispar, is non-pathogenic and accounts for about 90% of the previously estimated 500 million e. histolytica infections world-wide. because of the recent redefinition of e. histolytica and e. dispar, and the limited number of drugs available to treat amoebiasis, a new approach to treatment of individuals carrying these parasites is necessar ... | 2001 | 17061507 |
| a single proline substitution is critical for the thermostabilization of clostridium beijerinckii alcohol dehydrogenase. | analysis of the three-dimensional structures of three closely related mesophilic, thermophilic, and hyperthermophilic alcohol dehydrogenases (adhs) from the respective microorganisms clostridium beijerinckii (cbadh), entamoeba histolytica (ehadh1), and thermoanaerobacter brockii (tbadh) suggested that a unique, strategically located proline residue (pro100) might be crucial for maintaining the thermal stability of ehadh1. to determine whether proline substitution at this position in tbadh and cb ... | 2007 | 17063493 |
| relationship between free radicals produced by entamoeba histolytica and its proteases complex activity. | entamoeba histolytica is a parasite which causes health problems and there has been many approaches to know what is the factor causing its pathogenicity. in the present work, we assayed if the production of free radicals by the amoeba, has a relationship with the proteases activity. when we test the dmso action (free radicals quenching activity) the specific activity of the proteases complex of the parasite were affected also. at 33.3% (v/v) concentration of dmso it was present a maximal decreas ... | 2002 | 17063776 |
| irritable bowel syndrome: a review on the role of intestinal protozoa and the importance of their detection and diagnosis. | irritable bowel syndrome (ibs) is a functional gastrointestinal disorder in which abdominal pain is associated with a defect or a change in bowel habits. gut inflammation is one of the proposed mechanisms of pathogenesis. recent studies have described a possible role for protozoan parasites, such as blastocystis hominis and dientamoeba fragilis, in the etiology of ibs. dientamoeba fragilis is known to cause ibs-like symptoms and has a propensity to cause chronic infections but its diagnosis reli ... | 2007 | 17070814 |
| molecular modeling of some 1h-benzimidazole derivatives with biological activity against entamoeba histolytica: a comparative molecular field analysis study. | comparative molecular field analysis (comfa) was performed on a set of 1h-benzimidazole derivatives. molecular modeling and 3d-qsar were employed to determine the tautomeric form that would probably fit a target receptor in entamoeba histolytica. comfa results suggest that the antiamoebic activity is favored with steric bulk at position 5 of the benzimidazole ring and low electron density on the group at position 2. to the best of our knowledge this is the first 3d-qsar study performed for benzi ... | 2007 | 17074492 |
| subcellular distribution of the entamoeba histolytica 140 kda fn-binding molecule during host-parasite interaction. | entamoeba histolytica trophozoites recovered from the host-parasite interface during abscess development obtain different stimuli compared with long-term cultured cells. in order to have a better understanding about the mechanisms in which the 140 kda fibronectin (fn)-binding molecule (ehfnr) is involved during the invasive process, we decided to compare the regulation process of this molecule among long-term cultured trophozoites, fn-stimulated trophozoites, and trophozoites recently recovered ... | 2007 | 17076927 |
| pathogenicity of entamoeba dispar under xenic and monoxenic cultivation compared to a virulent e. histolytica. | two xenic isolates and cloned cultures of entamoeba dispar were submitted to monoxenization using crithidia fasciculata as the associated organism. growth in monoxenic cultivation and ability of xenic and monoxenic trophozoites to destroy vero cells and produce lesions in hamster livers were compared to those of a virulent e. histolytica. parental and cloned e. dispar under monoxenic cultivation showed a remarkable lower growth than the monoxenic e. histolytica and were avirulent in both in vivo ... | 2006 | 17086310 |
| additional antiprotozoal flavonol glycosides of the aerial parts of helianthemum glomeratum. | bioassay-guided fractionation of the methanol extract of aerial parts from helianthemum glomeratum afforded five antiprotozoal flavonol glycosides: tiliroside, kaempferol-3-o-(3'',6''di-o-e-p-coumaroyl)-betad-glucopyranoside, astragalin, quercitrin and isoquercitrin. the in vitro antiprotozoal assay showed that tiliroside was the most potent antiamoebic and antigiardial compound with ic(50) values of 17.5 microg/ml for entamoeba histolytica and 17.4 microg/ml for g. lamblia. isoquercitrin showed ... | 2007 | 17094176 |
| interaction of vibrio cholerae o139 with an intestinal parasite, entamoeba histolytica. | | 2006 | 17108284 |
| [intestinal parasitosis in french west indies: endemic evolution from 1991 to 2003 in the university hospital of pointe-a-pitre, guadeloupe]. | guadeloupe is one of the french west indies, where, until 1960, intestinal parasitic infections were endemic. in the microbiological laboratory of the university hospital of the island, we carried out a retrospective study of the 17,660 stool examinations received from 1991 to 2003. all the stool specimens were examined using at least a wet mount preparation and a concentration method (bailenger). specific techniques such as ziehl-neelsen modified acid fast stain, chromotrope staining procedure ... | 2006 | 17111973 |
| entamoeba histolytica: an ecto-phosphatase activity regulated by oxidation-reduction reactions. | in this work, an ecto-phosphatase activity of entamoeba histolytica was characterized using intact cells. this activity presented the following biochemical characteristics: (i) it hydrolyzes p-npp with v(max) of 8.00+/-0.22 nmol p-np x h(-1) x 10(-5) cells and k(m) of 2.68+/-0.25 mm; (ii) it is inhibited by acid phosphatase inhibitors, such as sodium molybdate (k(i)=1.70+/-0.24 microm) and sodium fluoride (k(i)=0.25+/-0.02 mm); (iii) it also showed high sensitivity to phosphotyrosine phosphatase ... | 2007 | 17113080 |
| mass treatment for intestinal helminthiasis control in an amazonian endemic area in brazil. | the objective of the present study was to estimate the prevalence of soil-transmitted helminthiasis and evaluate the sanitary conditions and the role of a mass treatment campaign for control of these infections in santa isabel do rio negro. a cross-sectional survey was carried out in 2002, to obtain data related to the sanitary conditions of the population and fecal samples for parasitological examination in 308 individuals, followed by a mass treatment with albendazole or mebendazole with cover ... | 2006 | 17119673 |
| [occurrence of entamoeba histolytica/entamoeba dispar in ambulatory patients of recife, pe]. | the objective this study was to determine the occurrence of the species entamoeba histolytica/entamoeba díspar in clinical samples of ambulatory patients in pernambuco. a specific assay for entamoeba histolytica was used in this study, which identified no positive fecal samples among the 213 patients. these results confirm that e. dispar is the dominant species in pernambuco state. | 2006 | 17119758 |
| evidence for a link between parasite genotype and outcome of infection with entamoeba histolytica. | the factors determining whether a person infected with entamoeba histolytica develops disease remain obscure. to investigate whether the parasite genome contributes to the outcome, we have investigated the distribution of parasite genotypes among e. histolytica-infected individuals in bangladesh. samples were obtained from individuals who either were asymptomatic, had diarrhea/dysentery, or had developed a liver abscess. genotypes were determined by using six trna-linked polymorphic markers, and ... | 2007 | 17122021 |
| infection by human immunodeficiency virus-1 is not a risk factor for amebiasis. | | 2006 | 17123964 |
| changes in bacterial profile during amebiasis: demonstration of anaerobic bacteria in ala pus samples. | little is known about the changes in gut resident flora during amebic colitis and amebic liver abscess (ala) caused by entamoeba histolytica infection. fecal samples from ala patients, from healthy e. histolytica negative and positive (asymptomatic) individuals, and from pre- and post-metronidazole-treated healthy volunteers and pus samples from ala patients were tested for the presence of various bacterial genera using 16s rrna-based primers. statistically significant reduction in lactobacillus ... | 2006 | 17123981 |
| [parasitic fauna and the frequency of entamoeba histolytica/entamoeba dispar detected by elisa in stool samples in sanliurfa, turkey]. | amoebiasis is a significant health problem in developing countries. humans are infected by two morphologically identical species of entamoeba. entamoeba histolytica causes amebic colitis and liver abscess, and entamoeba dispar is noninvasive. the aim of this study was to determine the prevalence of the e. histolytica/e. dispar complex using the elisa method on stools of patients. a total of 1600 stool specimens were examined using lugol preparations and the modified ritchie method. a total of 58 ... | 2006 | 17124656 |
| [distribution of intestinal parasites in children aged between 0 and 14 presenting at the laboratory of parasitology of the mustafa kemal university medical school.] | the aim of this study was to determine the parasite frequency in children from 0-14 years old who had presented at the parasitology laboratory of the mustafa kemal university medicine school complaining of gastrointestinal and anal pruritus. during the laboratory investigation, 561 fecal specimens and 534 cellophane tapes obtained from the patients were examined. after the microscope examination of 561 feces samples, it was found that 104 of these samples (from 50 boys and 54 girls) contained on ... | 2005 | 17124682 |
| [distribution of intestinal parasites among patients who presented at the department of parasitology of the erciyes university medical school.] | in this study carried out from 2000-2004, a total of 34,883 stool samples were examined using native-lugol and flotation/sedimentation methods and 9,879 cellophane tape preparations were examined directly. intestinal parasites were found in 9,704 (27.8%) of the specimens. the parasites that were found and their prevalence is as follows: blastocystis hominis, 6,723 (19.3%); entamoeba coli, 1,007 (2.9%); giardia intestinalis, 892 (2.6%); entamoeba histolytica/e. dispar, 798 (2.3%); endolimax nana, ... | 2005 | 17124684 |
| ehgef2, a dbl-rhogef from entamoeba histolytica has atypical biochemical properties and participates in essential cellular processes. | dbl proteins are a family of factors that exchange the guanine nucleotide which promote the activation of rho small gtpases. this paper reports the molecular, structural, biochemical and functional characterization of ehgef2, a new member of the dbl family. ehgef2 is the second gef studied in parasites and in the protozoan entamoeba histolytica, and it is also the first member of the dbl family that was found to have arm repeats. the catalytic domain (dh) of ehgef2 has the conserved residues t42 ... | 2007 | 17125853 |
| comparison of the proteome profiles of entamoeba histolytica and its close but non-pathogenic relative entamoeba dispar. | the microaerophilic protist entamoeba histolytica is a source of considerable morbidity and mortality in many countries of the world by acting as a causative agent of amoebic dysentery and liver abscess. its close relative, entamoeba dispar, also colonises the human colon but is non-pathogenic. in the present study, we wanted to assess if the close relatedness of these two organisms could be used to identify virulence factors in e. histolytica through a comparison of the protein expression profi ... | 2006 | 17131239 |
| entamoeba histolytica cell movement: a central role for self-generated chemokines and chemorepellents. | entamoeba histolytica cells, the cause of amoebic dysentery, are highly motile, and this motility is an essential feature of the pathogenesis and morbidity of amoebiasis. however, the control of e. histolytica motility within the gut and during invasion is poorly understood. we have used an improved chemotaxis assay to identify the key extracellular signals mediating entamoeba chemotaxis. the dominant responses we observe are caused by factors generated by e. histolytica cells themselves. medium ... | 2006 | 17132728 |
| locally acquired infection with entamoeba histolytica in men who have sex with men in australia. | | 2006 | 17137428 |
| oral fecal parasites and personal hygiene of food handlers in abeokuta, nigeria. | ingestion of infective eggs and cysts of faecal orally transmissible parasites has been linked with the level of environmental and personal hygiene. the possibility of contamination of food with eggs and cysts by infected food vendors has also been recorded. | 2006 | 17140338 |
| transcriptomic comparison of two entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin. | the availability of rahman, and the virulent hm-1:imss strain of e. histolytica, provides a powerful tool for identifying virulence factors of e. histolytica. here we report an attempt to identify potential virulence factors of e. histolytica by comparing the transcriptome of e. histolytica hm-1:imss and e. histolytica rahman. with phenotypically defined strains, we compared the transcriptome of rahman and hm-1:imss using a custom 70mer oligonucleotide based microarray that has essentially full ... | 2007 | 17141337 |
| decreased chemiluminescence in leukocyte adhesion deficiency presenting with recurrent sepsis, amoebiasis and candida albicans urinary tract infection. | leukocyte adhesion deficiency (lad) is a rare disorder of cellular immunity, generally due to various mutations producing reduced or altered expression of membrane integrins. the authors report a case of lad due to integrins expression imbalance. lad was suspected after recurrent sepsis, fungal infection and amoebiasis with persistent leukocytosis. neutrophils were studied with chemiluminescence showing decreased functional activity: up to now, this seems the first chemiluminescence study of neu ... | 2006 | 17146424 |
| intestinal parasites in dakahlia governorate, with different techniques in diagnosing protozoa. | a total of 3180 patients attending mansoura university hospitals' clinics, were subjected to stool examination by direct wet smear, formol-ether concentration, original formol-tween concentration, modified formol-tween concentration, modified sheather's sugar floatation, potassium hydroxide concentration and gomori's trichrome stain, and modified kinyoun's acid-fast stain, and ryan's trichrome blue stain for microsporidia. the intestinal helminthes in a descending order of abundance were: s. man ... | 2006 | 17153711 |
| syntheses, characterization and in vitro antiamoebic activity of new pd(ii) complexes with 1-n-substituted thiocarbamoyl-3,5-diphenyl-2-pyrazoline derivatives. | reaction of neutral ns bidentate ligands, 1-n-substituted thiocarbamoyl-3,5-diphenyl-2-pyrazolines, isolated by cyclization of chalcone with n-4-substituted thiosemicarbazide of aromatic amines (1-8), with [pd(dmso)(2)cl(2)] (dmso=dimethylsulfoxide) leads to the formation of new complexes of the type [pd(l)cl(2)] (1a-8a). the structures of the compounds were elucidated by uv, ir, (1)h nmr, (13)c nmr and esi-ms spectral data and thermogravimetric analysis and their purities were confirmed by elem ... | 2007 | 17156895 |
| antiparasitic drug nitazoxanide inhibits the pyruvate oxidoreductases of helicobacter pylori, selected anaerobic bacteria and parasites, and campylobacter jejuni. | nitazoxanide (ntz) exhibits broad-spectrum activity against anaerobic bacteria and parasites and the ulcer-causing pathogen helicobacter pylori. here we show that ntz is a noncompetitive inhibitor (k(i), 2 to 10 microm) of the pyruvate:ferredoxin/flavodoxin oxidoreductases (pfors) of trichomonas vaginalis, entamoeba histolytica, giardia intestinalis, clostridium difficile, clostridium perfringens, h. pylori, and campylobacter jejuni and is weakly active against the pyruvate dehydrogenase of esch ... | 2007 | 17158936 |
| [evaluation of the distribution of intestinal parasites in the uludag university medical faculty during a period of eight years.]. | the purpose of the present study was to investigate retrospectively the distribution and prevalence of intestinal parasites in patients who presented at the routine parasitology laboratory of the uludag university medical faculty, bursa, during the 8 years from january 1993-december 2000. a total of 32,346 stool specimens were examined for intestinal parasites using native- lugol and formol ethyl ether methods. also, 10,897 cellophane- tape preparations were studied directly. parasites were foun ... | 2005 | 17160822 |
| [investigation of intestinal parasites in residents of the kayseri karpuzsekisi basin]. | in this study, we collected stool and cellophane tape samples from residents in four villages located in the kayseri-karpuzsekisi basin in order to investigate the distribution of intestinal parasites. a total of 240 samples collected from hanyeri (40), sariküklü (20), dokuzpinar (80) and karpuzsekisi (120) were investigated. the parasites detected were as follows: blastocystis hominis, 82 (34.16%); enterobius vermicularis, 37 (15.41%); entamoeba coli 26, (10.83%); entamoeba hartmanni, 10 (4.16% ... | 2006 | 17160846 |
| [incidence of intestinal parasites in municipal sanitary workers in malatya]. | the incidence of intestinal parasites is closely related to such factors as the socio-economic level of the society, nutritional and hygienic habits, climate, environmental conditions, infrastructure and degree of literacy. in this study, the municipal sanitary workers who are regarded as a high risk group in malatya were examined for intestinal parasites. cellophane slides and fecal samples from 241 workers were examined and intestinal parasites were found in 93 (39.0%). the most common parasit ... | 2006 | 17160847 |
| cryptosporidiosis and other intestinal protozoan infections in children less than one year of age in mexico city. | parasitic diseases are very important in mexico because of their economic impact and adverse effects on normal growth in children. cryptosporidiosis has been associated with acute diarrhea in immune competent and incompetent human hosts, fecal contamination of drinking water sources, and handling of animals. due to the lack of reports on cryptosporidiosis in mexico, we conducted a parasitologic study in children with diarrhea and other clinical symptoms. the main objectives were 1) to determine ... | 2006 | 17172373 |
| isolation of pure compound r/j/3 from pluchea indica (l.) less. and its anti-amoebic activities against entamoeba histolytica. | the plant pluchea indica is known for its anti-inflammatory, anti-ulcer, anti-pyretic, hypoglycemic, diuretic and anti-microbial activities besides many other pharmacological activities. we have isolated and purified seven compounds from the methanolic root extract of this plant by column chromatography. the compounds were identified by spectroscopic analyses. the anti-amoebic activities of the pure compound r/j/3 was investigated against the hm1 strain of entamoeba histolytica. the compound, r/ ... | 2007 | 17174538 |
| sine elements of entamoeba dispar. | entamoeba histolytica and e. dispar are closely related protozoan parasites; the former causes clinical amoebiasis in humans while the latter appears to be non-pathogenic. the molecular biology of e. histolytica shows a number of unusual features, one of which is the abundance of polyadenylated but apparently untranslatable mrnas produced; many of these are the product of at least three families of sines (ehsine1-3). here we show that the genome of e. dispar contains numerous copies of a sine el ... | 2007 | 17178164 |
| incidence of cyclosporiasis in patients with gastrointestinal symptoms in western turkey. | this study was designed to investigate the distribution of cyclosporiasis between october 2003 and october 2004 and the relationship between cyclospora infection and seasonal as well as patient factors in western turkey. | 2007 | 17179908 |
| innate resistance to entamoeba histolytica in murine models. | hamano et al. have recently reported that in murine models of amebiasis, innate resistance is associated with nonhemopoietic cells and depends on the activity of interleukin-10. this article raises the issue of the importance of predisposition to amebic colitis and discusses which host factors have a role in innate resistance to invasive disease. resistance studies that elucidate the mechanisms involved in parasite clearance or parasite invasion are essential to understanding the incidence of am ... | 2007 | 17185037 |
| initiation of inflammation and cell death during liver abscess formation by entamoeba histolytica depends on activity of the galactose/n-acetyl-d-galactosamine lectin. | the parasite entamoeba histolytica colonizes the human intestine causing amoebic colitis and disseminates through the vascular route to form liver abscesses. the gal/galnac lectin is an adhesion protein complex which sustains tissue invasion by e. histolytica. disruption of the gal/galnac lectin function in engineered parasites (hgl-2 trophozoites) changed the pathophysiology of hamster liver abscess formation. hgl-2 trophozoites produced numerous small inflammatory foci located in the vicinity ... | 2007 | 17188278 |
| molecular methods for diagnosis of entamoeba histolytica in a clinical setting: an overview. | the range of clinical outcomes following entamoeba histolytica infection is likely to be influenced by the different strains of the parasite already existing in our population. there is a need for developing faster, reliable and reproducible methods for identifying the different strains of e. histolytica. this would have a major impact on the subsequent course of treatment given to patients. in the post-genomic era, different loci of the entamoeba genome have been targeted for developing suitabl ... | 2007 | 17189632 |
| synthesis and anti-amoebic activity of gold(i), ruthenium(ii), and copper(ii) complexes of metronidazole. | a series of au, ru, and cu complexes of metronidazole (= [1-(2-hydroxyethyl)-2-methyl-5-nitro-1h-imidazole; 1) were prepared as highly potent anti-amoebic drugs. the complexes [au(pph3)(1)]pf6 (2), [ru(1)2(cl)2(h2o)2] (3), and [cu(1)2(mu-cl)(h2o)]2cl2 (4) were readily synthesized from [au(pph3)cl], rucl3 x 3 h2o, and cucl2 x 2 h2o, respectively. all complexes were thoroughly characterized by ir, uv/vis, 1h-nmr, fab-ms, elemental and thermogravimetric analyses, and, in the case of 4, also by x-ra ... | 2005 | 17191933 |
| colonic amoebiasis and spirochetosis: morphological, ultrastructural and microbiological evaluation. | the present study reports on a prompt diagnosis of colonic amoebiasis with colonic spirochetosis by brachyspira aalborgi and b. pilosicoli; such diagnosis allowed exclusion of other diseases and resolution of the case after specific treatment. | 2007 | 17201883 |
| parasitic etiology of childhood diarrhea. | the objective was to determine the prevalence of parasitic agents among under-five children with diarrhea in ilesa, nigeria and the clinical correlates of diarrhea associated with parasitic infestation. all under-five children presenting with diarrhea in the hospital had stool microscopic examination. children with parasites in diarrheic stools (cases) were compared with those without (controls) for clinical features. out of 300 under-five children with diarrhea, 70 (23.3%) had parasites. there ... | 2006 | 17202634 |
| growth of the protozoan parasite entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression. | in higher eukaryotes dna methylation regulates important biological functions including silencing of gene expression and protection from adverse effects of retrotransposons. in the protozoan parasite entamoeba histolytica, a dna methyltransferase has been identified and treatment with 5-azacytidine (5-azac), a potent inhibitor of dna methyltransferase, has been reported to attenuate parasite virulence. however, the overall extent of dna methylation and its subsequent effects on global gene expre ... | 2007 | 17207281 |
| association of parasites and diarrhoea among children less than five years of age in a rural area in egypt. | a case control study was conducted in kafr el-sheikh governorate, egypt, a rural community, to assess the relation of parasites and diarrhoea among children <5 years. a sample of 196 cases with diarrhoea and 83 non-diarrhoeic controls were included in the study. a questionnaire was completed for every child and weight and height were recorded. a stool sample was obtained and examined for parasites present. the results revealed that 70.3% of cases had parasites compared to 59% of controls. the mo ... | 1996 | 17214190 |
| activation of dendritic cells by the gal-lectin of entamoeba histolytica drives th1 responses in vitro and in vivo. | amebiasis is a human disease caused by the protozoan intestinal parasite entamoeba histolytica. vaccine development has focused on the parasite's surface galactose-n-acetyl-d-galactosamine inhibitable lectin (gal-lectin) as a protective antigen. the gal-lectin is immunogenic and has been shown to induce th1 cytokines in vitro and in vivo. the immunological basis of the protective immune response elicited by the gal-lectin is unknown. in this study, we investigated the response of balb/c bone mar ... | 2007 | 17219364 |
| current therapeutics, their problems, and sulfur-containing-amino-acid metabolism as a novel target against infections by "amitochondriate" protozoan parasites. | the "amitochondriate" protozoan parasites of humans entamoeba histolytica, giardia intestinalis, and trichomonas vaginalis share many biochemical features, e.g., energy and amino acid metabolism, a spectrum of drugs for their treatment, and the occurrence of drug resistance. these parasites possess metabolic pathways that are divergent from those of their mammalian hosts and are often considered to be good targets for drug development. sulfur-containing-amino-acid metabolism represents one such ... | 2007 | 17223627 |
| pcr detection of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii in stool samples from sydney, australia. | this study investigated the presence of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii in stool samples from a patient population in sydney, australia. stool samples were tested by microscopy and pcr. five patients were found with e. histolytica infections, while e. dispar and e. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by pcr. this is the first study in australia using molecular techniques to determine the presence of e. histolytica, e. ... | 2007 | 17229864 |
| fulminant amoebic colitis following loperamide use. | toxic megacolon is a rare consequence of infection with entamoeba histolytica. we present such a patient in whom the course of disease may have been influenced by heavy loperamide use. loperamide and other anti-motility agents have been implicated previously in the pathogenesis of toxic megacolon in patients with infectious gastroenteritis. | 2007 | 17241255 |
| identification of developmentally regulated genes in entamoeba histolytica: insights into mechanisms of stage conversion in a protozoan parasite. | developmental switching between life-cycle stages is a common feature among many pathogenic organisms. the protozoan parasite entamoeba histolytica converts between cysts (essential for disease transmission) and trophozoites (responsible for tissue invasion). identification of genes involved in the developmental pathway has been severely hindered by the inability to generate e. histolytica cysts in vitro. using parasite strains derived from recent human infections and whole-genome transcriptiona ... | 2007 | 17250591 |
| luminal host-defense mechanisms against invasive amebiasis. | most humans infected with the virulent protozoan parasite entamoeba histolytica do not develop invasive disease. available evidence indicates that beneficial bacteria and the mucus gel layer in the colon lumen protect the host mucosa. glycosidases produced by some normal colonic bacteria and luminal proteases degrade the key adherence lectin on e. histolytica trophozoites and decrease their adherence to epithelial cells. the mucus gel layer prevents those trophozoites that escape the hydrolases ... | 2007 | 17258936 |
| anemia, parasitic infections and some risk factors among physical education female students in alexandria. | the present study was a cross-sectional one, carried out to estimate the percentages of anemia and parasites among students of faculty of physical education for girls and some of their related factors. a predesigned questionnaire was completed by the researchers through face-to-face meetings to collect data concerning age, menstruation history and some dietary habits. blood samples were taken by finger pricks to estimate hemoglobin (hb) concentration level (gm/dl). stool samples were collected a ... | 2003 | 17265613 |
| vaccines for amoebiasis: barriers and opportunities. | amoebiasis, infection by the protozoan parasite entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. while improved sanitation could lead to the eradication of this disease, it is unlikely that this will occur worldwide in the foreseeable future; thus alternative measures must be pursued. one approach is to develop a vaccine to prevent this deadly disease. clinical studies indicate that mucosal immunity may provide some protection against recur ... | 2006 | 17274850 |
| invasive amebiasis. | digestive amoebiasis with his invasive form is an unusual pathology encountered in the temperate zone. this could lead to a life threatening complication: systemic amoebiasis. a 55-year-old male was treated successfully of systemic amoebiasis in a third referral hospital. the diagnosis was established based on epidemiology data and microscopical identification of trophozoites of entamoeba histolytica. the amoebicidal, antibiotic and supportive treatments was firstly administrated. the clinical p ... | 2006 | 17278650 |
| induction of monocyte chemotactic protein 1 in colonic epithelial cells by entamoeba histolytica is mediated via the phosphatidylinositol 3-kinase/p65 pathway. | the role intestinal epithelial cells play in the pathogenesis of amebic colitis is poorly understood. herein, we demonstrate that secreted and soluble ameba (entamoeba histolytica) proteins (sap) induce expression of the chemoattractant monocyte chemotactic protein (mcp) in the colonic epithelial cell lines caco-2, t84, and ls174t. mcp-1 mrna induction was both dose and time dependent, with peak induction occurring at 8 h and with 100 mug/ml of sap. significant increase in mcp-1 protein expressi ... | 2007 | 17283105 |
| correlation of interferon-gamma production by peripheral blood mononuclear cells with childhood malnutrition and susceptibility to amebiasis. | the contribution of interferon-gamma (ifn-gamma) to immunity from amebiasis was assessed in a three-year prospective study of children 2-5 years of age in an urban slum of dhaka, bangladesh. ifn-gamma produced by peripheral blood mononuclear cells stimulated with soluble amebic antigen was measured upon enrollment. thirty-one of the 209 enrolled children had entamoeba histolytica-associated diarrhea. children who produced higher than the median level of ifn-gamma (median = 580 pg/ml) had longer ... | 2007 | 17297046 |
| onchocerca volvulus-specific antibody and cytokine responses in onchocerciasis patients after 16 years of repeated ivermectin therapy. | the recommended control option against onchocerciasis is repeated ivermectin treatment, which will need to be implemented for decades, and it remains unknown how repeated ivermectin therapy might affect immunity against onchocerca volvulus in the long term. o. volvulus-specific antibody reactivity and cellular cytokine production were investigated in onchocerciasis patients receiving ivermectin (150 microg/kg) annually for 16 years. in treated patients, the t helper type 2 (th2) cytokine interle ... | 2007 | 17302900 |
| the 29-kilodalton thiol-dependent peroxidase of entamoeba histolytica is a factor involved in pathogenesis and survival of the parasite during oxidative stress. | the 29-kda surface antigen (thiol-dependent peroxidase; eh29) of entamoeba histolytica exhibits peroxidative and protective antioxidant activities. during tissue invasion, the trophozoites are exposed to oxidative stress and need to deal with highly toxic reactive oxygen species (ros). in this investigation, attempts have been made to understand the role of the 29-kda peroxidase gene in parasite survival and pathogenesis. inhibition of eh29 gene expression by antisense rna technology has shown a ... | 2007 | 17307964 |
| parasitism in kansas in the 1800s: a glimpse to the past through the analysis of grave sediments from meadowlark cemetery. | during the excavations of the xix century meadowlark cemetery (manhattan, kansas, us), samples of sediments were taken from around five skeletons, and analyzed to detect intestinal parasites. no helminth eggs were found, but immunological elisa tests for entamoeba histolytica were positive in three samples. the immunological techniques have been successfully used in paleoparasitology to detect protozoan infections. amoebiasis could have been a severe disease in the past, especially where poor sa ... | 2006 | 17308809 |
| [the distribution of patients with intestinal parasites presenting at the parasitology laboratory of the mustafa kemal university medical faculty]. | in this study, fecal specimens of 3,679 patients presenting at the parasitology laboratory of the medical faculty of the mustafa kemal university between september and may 2005 were examined for intestinal parasites. a total 3,679 stool specimens from 1,260 (34.3%) males and 2,419 (65.7%) females for intestinal parasites using the native-lugol method were examined. intestinal parasites were found in 265 (34.23%) male and 509 (65.76%) females. one or more intestinal parasites were detected in 774 ... | 2006 | 17309033 |
| [comparison of the results of examination of fecal samples from students at six months intervals in the alahaci village primary school in sivas]. | the aim of this study was to determine the distribution of intestinal parasites in primary school children in the alahaci central village in the sivas province, but owing to high prevalence of parasites in soil, new stool specimens were taken from the same subject group after 6 months. in the first stage of investigation, stool samples from a total of 189 children were taken and examined. it was found that the 110 (58.2%) out of 189 subjects were infected with parasites. the distribution of para ... | 2006 | 17309034 |
| [the distribution of intestinal parasites detected in the dokuz eylul university medical faculty hospital between 2003 and 2004]. | a retrospective evaluation of the data from 7,712 patients with gastrointestinal complaints who presented at the parasitology laboratory of the dokuz eylul university medical faculty hospital between january 2003 and december 2004 was carried out. fecal samples of all patients were examined using native-lugol and the trichrome staining method after sedimentation by the fecal concentration tube. one or more parasites were detected in 495 (6.41%) of the patients. the distribution of the intestinal ... | 2006 | 17309035 |
| stable expression of gal/galnac lectin of entamoeba histolytica in transgenic chloroplasts and immunogenicity in mice towards vaccine development for amoebiasis. | chloroplast genetic engineering offers several advantages, including high levels of transgene expression, transgene containment via maternal inheritance and multigene engineering in a single transformation event. entamoeba histolytica infects 50 million people, causing about 100,000 deaths annually, but there is no approved vaccine against this pathogen. leca, a potential target for blocking amoebiasis, was expressed for the first time in transgenic plants. stable transgene integration into chlo ... | 2007 | 17309678 |
| prevalence of intestinal parasites in the area of parma during the year 2005. | intestinal parasitosis represent a relevant clinical problem, especially in developing countries, where they are responsible for morbidity and mortality in adults and children and many epidemiological data are available for these areas. the actual situation of intestinal parasitosis in europe is not yet well investigated since they are usually not notified. we describe the occurrence of intestinal parasitosis in our laboratory from january to december 2005. | 2006 | 17312984 |
| magnesium promotes structural integrity and conformational switching action of a calcium sensor protein. | calcium binding proteins carry out various signal transduction processes upon binding to ca2+. in general, these proteins perform their functions in a high background of mg2+. here, we report the role of mg2+ on a calcium sensor protein from entamoeba histolytica (ehcabp), containing four ca2+-binding sites. mg2+-bound ehcabp exists as a monomer with a conformation different from that of the holo- and apo-ehcabp. nmr and biophysical data on ehcabp demonstrate that mg2+ stabilizes the closed conf ... | 2007 | 17315953 |
| importance of the detection of amoebic antigens in stool samples for the diagnosis of entamoeba histolytica infection, among children in southern turkey. | entamoeba histolytica is the predominant causative agent of human amoebiasis, a significant and common diarrhoeal disease among children of developing countries. diagnosis of this illness by the microscopical detection of the parasites in stool samples is insensitive and often incapable of differentiating the pathogenic entamoeba histolytica from the commensal parasite e. dispar. in this study, the results of testing stool samples in an elisa, based on a monoclonal antibody, that detects e. hist ... | 2007 | 17316500 |
| differentiation of entamoeba histolytica and entamoeba dispar by pcr: a preliminary study in izmir, turkey. | the causative agent of amoebiasis is currently attributed to two distinct species (e. histolytica and e. dispar). the aim of this study was to differentiate these species by pcr in stool samples. isolated genomic dna was amplified by pcr and band products of 101 bp (e. dispar) were obtained. all seven stool samples were found to be e. dispar, not e. histolytica. our results demonstrated the significance of e. histolytica/dispar differentiation in the diagnosis of amoebiasis. this study is prelim ... | 2007 | 17319599 |
| ehgef3, a novel dbl family member, regulates ehraca activation during chemotaxis and capping in entamoeba histolytica. | rho gtpases are critical elements involved in the regulation of signal transduction cascades from extracellular stimuli to cytoskeleton. the rho guanine nucleotide exchange factors (rhogefs) have been implicated in direct activation of these gtpases. here, we describe a novel rhogef, denominated ehgef3 from the parasite entamoeba histolytica, which encodes a 110 kda protein containing the domain arrangement of a dbl homology domain in tandem with a pleckstrin homology domain, the dh domain of eh ... | 2007 | 17323375 |
| frequency of giardia lamblia among children in dohuk, northern iraq. | out of 1261 stool specimens collected from children in dohuk city, northern iraq, the prevalence of giardia lamblia infection was 38.5%. the highest rate of infection was in orphan care centres (48.1%) and the lowest in the paediatric hospital (31.3%). the age group 10-12 years had the highest rate (81.2%) and 7-9 years the lowest (22.9%); boys had a higher rate than girls. some infected samples (70/486) showed double or triple infections and g. lamblia was combined with hymenolepis nana, blasto ... | 2006 | 17333793 |
| enteroparasitic occurrence in stools from residents in southwestern region of saudi arabia before and during umrah season. | study of the prevalence of human gastro-intestinal parasitic infections among patients living in makkah al-mukkarmah city before and during umrah season. | 2007 | 17334465 |
| entamoeba histolytica: apoptosis induced in vitro by nitric oxide species. | apoptosis has been described in some parasites like leishmania, trypanosoma, and trichomonas. this phenomenon has not been observed yet in entamoeba histolytica. this work analyzed the in vitro effect of sodium nitroprusside, sodium nitrite and sodium nitrate (nos) on e. histolytica apoptosis. parasites incubated for 1h with nos revealed apoptosis 6h later (95% viability), demonstrated by yopro-1, tunel, dna fragmentation and low atp levels. the caspase inhibitor z-vad-fmk inhibited total intrac ... | 2007 | 17336295 |
| nitric oxide production and nitric oxide synthase immunoreactivity in naegleria fowleri. | free-living ameba naegleria fowleri produces an acute and fatal infectious disease called primary amebic meningoencephalitis (pam), whose pathophysiological mechanism is largely unknown. the aim of this study was to investigate the role of nitric oxide (no) in pam. although no has a cytotoxic effect on various parasites, it is produced by others as part of the pathology, as is the case with entamoeba histolytica. to test for the production of no, we analyzed whether antibodies against mammalian ... | 2007 | 17340143 |
| [study on genetic approach in the detection of cryptosporidium parvum and giardia lamblia in acquired immunodeficiency syndrome patients]. | to establish genetic method in detecting cryptosporidium parvum and giardia lamblia which often coinfected with aids patients. | 2006 | 17343184 |
| identification of putative transcriptional regulatory networks in entamoeba histolytica using bayesian inference. | few transcriptional regulatory networks have been described in non-model organisms. in entamoeba histolytica seminal aspects of pathogenesis are transcriptionally controlled, however, little is known about transcriptional regulatory networks that effect gene expression in this parasite. we used expression data from two microarray experiments, cis-regulatory motif elucidation, and a naïve bayesian classifier to identify genome-wide transcriptional regulatory patterns in e. histolytica. our algori ... | 2007 | 17355990 |
| comparison of entamoeba histolytica dna isolated from a cynomolgus monkey with human isolates. | three protein-coding loci in dna of an entamoeba histolytica strain (ehmfas1) isolated from cynomolgus monkey (macaca fascicularis) were sequenced; these loci corresponded to the genes for chitinase, the serine-rich e. histolytica protein (srehp), and the 16 s-like small subunit ribosomal rna (16s-like ssurrna). the nucleotide and deduced amino-acid sequences of chitinase and srehp were compared with sequences from human isolates. ehmfas1 had several specific mutations in units in the polymorphi ... | 2007 | 17356893 |
| intestinal parasitosis among the elderly people in kathmandu valley. | present study was carried out among the elderly people (60+ years of age) from august 2005 to july 2006 in kathmandu valley to assess the prevalence of intestinal parasitosis in them. stool samples were collected from 235 elderly people (122 from government elderly home, 66 from private elderly home and 47 from the households in a rural community). the samples were examined by formal ether sedimentation and sheather's sucrose floatation followed by kinyoun's modified ziehl-neelsen staining. the ... | 2006 | 17357641 |
| two rab7 isotypes, ehrab7a and ehrab7b, play distinct roles in biogenesis of lysosomes and phagosomes in the enteric protozoan parasite entamoeba histolytica. | rab7 small gtpase plays a crucial role in the regulation of trafficking to late endosomes, lysosomes and phagosomes. while most eukaryotes encode a single rab7, the parasitic protist entamoeba histolytica possesses nine rab7. in this study, to understand the significance of the presence of multiple rab7 isotypes, a role of two representative rab7 isotypes, ehrab7a and ehrab7b, was investigated. ehrab7b was exclusively localized to acidic vacuoles containing lysosomal proteins, e.g. amoebapore-a ... | 2007 | 17359234 |
| prevalence of enteric protozoa in human immunodeficiency virus (hiv)-positive and hiv-negative men who have sex with men from sydney, australia. | a prospective, comparative study of the prevalence of enteric protozoa was determined among human immunodeficiency virus (hiv)- positive and hiv-negative men who have sex with men (msm) in sydney, australia. a total of 1,868 patients submitted stool specimens; 1,246 were from msm (628 hiv positive and 618 hiv positive) and 622 from non-msm were examined over a 36-month period. a total of 651 (52.2%) stool specimens from msm were positive for protozoa compared with 85 (13%) from non-msm. there wa ... | 2007 | 17360882 |
| late experimental amebic liver abscess in hamster is inhibited by cyclosporine and n-acetylcysteine. | during early experimental amebic liver abscess in hamsters (ealah), acute inflammation is primarily responsible for tissue damage. however, during the late stages of this process, the relative contribution to tissue destruction of both parasite factors and host response is unknown. in the present work, the role of the cellular immune response in tissue damage during ealah is explored by using the immunosuppressor drug cyclosporine a (csa). csa treatment inhibits tissue damage after 72 h (but not ... | 2007 | 17362925 |