| serological survey of <i>brucella canis</i> in dogs in urban harare and selected rural communities in zimbabwe. | a cross-sectional study was conducted in order to detect antibodies for brucella canis (b. canis) in dogs from urban harare and five selected rural communities in zimbabwe. sera from randomly selected dogs were tested for antibodies to b. canis using an enzyme-linked immunosorbent assay. overall, 17.6% of sera samples tested (57/324, 95% ci: 13.5-21.7) were positive for b. canis antibodies. for rural dogs, seroprevalence varied from 11.7% - 37.9%. rural dogs recorded a higher seroprevalence (20. ... | 2014 | 28235300 |
| integrating population- and individual-level information in a movement model of yellowstone bison. | throughout the world, fragmentation of landscapes by human activities has constrained the opportunity for large herbivores to migrate. conflict between people and wildlife results when migrating animals transmit disease to livestock, damage property, and threaten human safety. mitigating this conflict requires understanding the forces that shape migration patterns. bison bos bison migrating from yellowstone national park into the state of montana during winter and spring concern ranchers on land ... | 2014 | 24689146 |
| identification of novel srnas in brucella abortus 2308. | involved in diverse biological processes, bacterial srnas are novel regulators of gene expression involved in a wide array of biological processes. to identify srnas in brucella abortus, we performed a genome-wide computational prediction with integrated sipht and napp results. in total, 129 srna candidates were identified, of which 112 were novel srna. twenty novel srna candidates were tested by rt-pcr and seven could be verified. the putative targets of these srnas were also predicted and veri ... | 2014 | 24673258 |
| [construction of brucella abortus a19-delta virb12 mutant and evaluation of its protective efficacy against 2308 strain challenge in balb/c mice]. | a19-deltavirb12 deletion mutant of brucella abortus was constructed by using homologous recombination technology. balb/c mice were vaccinated intraperitoneally with the mutant to evaluate protective efficacy against brucella abortus 2308 challenge. | 2013 | 24617263 |
| characterization of the immunogenicity and pathogenicity of malate dehydrogenase in brucella abortus. | brucella abortus is a gram-negative, facultative intracellular pathogen that causes brucellosis, a chronic zoonotic disease resulting in abortion in pregnant cattle and undulant fever in humans. malate dehydrogenase (mdh), a key enzyme in the tricarboxylic acid cycle, plays important metabolic roles in aerobic energy producing pathways and in malate shuttle. in this study, the mdh-encoding gene for malate dehydrogenase mdh of b. abortus s2308 was cloned, sequenced and expressed. western blot ana ... | 2014 | 24609497 |
| induction of immune responses by two recombinant proteins of brucella abortus, outer membrane proteins 2b porin and cu/zn superoxide dismutase, in mouse model. | the diagnosis of brucella abortus is mainly based on serological methods using antibody against lps, which has diagnostic problems. therefore, to solve this problem, we evaluated two proteins of b. abortus, cu/zn superoxide dismutase (sodc) and outer membrane proteins 2b porin (omp2b). the genes were cloned and expressed in a pmal system, and the recombinant proteins, romp2b and rsodc, were purified as fusion forms with maltosebinding protein. the identity of the proteins was confirmed by sds-pa ... | 2014 | 24608566 |
| novel influenza virus vectors expressing brucella l7/l12 or omp16 proteins in cattle induced a strong t-cell immune response, as well as high protectiveness against b. abortus infection. | this paper presents the results of a study of the immunogenicity and protectiveness of new candidate vector vaccine against brucella abortus - a bivalent vaccine formulation consisting of a mixture of recombinant influenza a subtype h5n1 or h1n1 (viral constructs vaccine formulation) viruses expressing brucella ribosomal protein l7/l12 and omp16, in cattle. to increase the effectiveness of the candidate vaccine, adjuvants such as montanide gel01 or chitosan were included in its composition. immu ... | 2014 | 24598723 |
| express immunochromatographic detection of antibodies against brucella abortus in cattle sera based on quantitative photometric registration and modulated cut-off level. | an immunochromatographic test system was developed for rapid detection of the levels of specific igg antibodies to brucella abortus lipopolysaccharide, as a tool for diagnosis of brucellosis in cattle. the pilot test strips were examined using blood sera from sick (78 samples) and healthy (35 samples) cows. the results obtained by immunochromatographic assay, using a portable optical densitometer for digital video detection, correlate well with the results obtained by immunoenzyme assay and are ... | 2015 | 24579979 |
| seroprevalence for coxiella burnetii, francisella tularensis, brucella abortus and brucella melitensis in austrian adults: a cross-sectional survey among military personnel and civilians. | the prevalence of coxiella burnetii, francisella tularensis, brucella abortus, and brucella melitensis infections in austria and the exposure risk of military personnel were assessed in an exploratory nationwide cross-sectional seroprevalence survey in 526 healthy adult individuals, 222 of which were soldiers and 304 were civilians. screening for iga/igg antibodies to c. burnetii (phase i) and igg/igm antibodies to c. burnetii (phase ii), and to f. tularensis was done with commercial enzyme-link ... | 2014 | 24572607 |
| the influence of genetic background versus commercial breeding programs on chicken immunocompetence. | immunocompetence of livestock plays an important role in farm profitability because it directly affects health maintenance. genetics significantly influences the immune system, and the genotypic structure of modern fast-growing chickens has been changed, particularly after decades of breeding for higher production. therefore, this study was designed to help determine if intensive breeding programs have adversely affected immunocompetence or whether the immune response profiles are controlled to ... | 2014 | 24570426 |
| feasibility of quarantine procedures for bison (bison bison) calves from yellowstone national park for conservation of brucellosis-free bison. | objective--to determine the feasibility of qualifying individuals or groups of yellowstone national park bison as free from brucellosis. design--cohort study. sample--serum, blood, and various samples from live bison and tissues taken at necropsy from 214 bison over 7 years. procedures--blood was collected from bison every 30 to 45 days for serologic tests and microbiological culture of blood for brucella abortus. seropositive bison were euthanized until all remaining bison had 2 consecutive neg ... | 2014 | 24548234 |
| comparative whole genome analysis of six diagnostic brucellaphages. | whole genome sequencing of six diagnostic brucellaphages, tbilisi (tb), firenze (fz), weybridge (wb), s708, berkeley (bk) and r/c, was followed with genomic comparisons including recently described genomes of the tb phage from mexico (tbm) and pr phage to elucidate genomic diversity and candidate host range determinants. comparative whole genome analysis revealed high sequence homogeneity among these brucellaphage genomes and resolved three genetic groups consistent with defined host range pheno ... | 2014 | 24530704 |
| comparative proteomics analysis of host cells infected with brucella abortus a19. | we carried out a proteomic analysis of thp-1-derived macrophages with and without brucella abortus a19 (b. abortus a19) infection in order to study the cellular responses to b. abortus a19. the proteins were analyzed at different time points after infection with 2de followed by maldi-tof/tof identification. comparative analysis of multiple 2de gels revealed that the majority of changes in protein abundance appeared between 48 and 96 h after infection. ms identified 44 altered proteins, including ... | 2014 | 24519676 |
| a comparative assessment of culture and serology in the diagnosis of brucellosis in dairy cattle. | to investigate the usefulness of culture for the confirmation of brucellosis in cattle, a comparison of culture and serology was undertaken on 248 animals in four dairy herds where the disease was active. paired supramammary (sm), retropharyngeal (rp), and internal iliac (il) lymph nodes were cultured, and five serological tests were deployed: the microserum agglutination test (msat), complement fixation test (cft), the indirect (ielisa) and competitive elisa, and the fluorescence polarisation a ... | 2014 | 24507882 |
| outbreak of laboratory-acquired brucella abortus in brazil: a case report. | human brucellosis is an occupational disease affecting workers in slaughterhouses, butcher shops and the milk and dairy product industry as well as individuals who work in clinical or research laboratories. we report the first outbreak of a brucella abortus infection in a brazilian laboratory and compare the data obtained with reports available in the literature. exposure was a result of damage to a biological safety cabinet and failure of the unidirectional airflow ventilation system. an epidem ... | 2014 | 24474027 |
| the use of contraception as a disease management tool in wildlife. | contraception offers potential as a tool for managing certain diseases in wildlife, most notably venereally transmitted diseases or diseases transmitted at parturition. brucellosis is an excellent example of an infectious disease present in wild populations that could potentially be managed through immunocontraception. previous studies in bison (bison bison) suggest that a single injection of gonacon (national wildlife research center, u.s. department of agriculture/animal and plant health inspe ... | 2013 | 24437094 |
| in vivo-induced argininosuccinate lyase plays a role in the replication of brucella abortus in raw264.7 cells. | brucellosis caused by brucella species is a zoonotic disease with a serious impact on public health and the livestock industry. to better understand the pathogenesis of the disease, in vivo-induced antigen technology (iviat) was used to investigate the in vivo-induced antigens of brucella abortus in this study. a genomic expression library of b. abortus was constructed and screened using pooled bovine b. abortus-positive sera by iviat. in total, 33 antigens were identified. five antigens were fu ... | 2014 | 24421405 |
| human brucellosis in northwest ecuador: typifying brucella spp., seroprevalence, and associated risk factors. | human brucellosis in ecuador is underreported and based only on passive surveillance. since 2008, brucellosis was removed from the list of communicable diseases in the country. until now, the true human brucellosis picture has not yet been determined. the aim of this study was to determine the seroprevalence of the disease, identify risk factors associated with brucellosis seropositivity in humans, and isolate circulating strains of brucella spp. in the northwestern part of ecuador. between 2006 ... | 2014 | 24410144 |
| [detection of a clonal complex with brucella abortus biovar 2 genotype as founder in b. abortus isolates from argentina]. | brucella abortus is the causative agent of bovine brucellosis, a worldwide zoonosis. up to date, eight biovars of b. abortus have been described. in argentina, biovar 1 is the most frequently isolated. however, biovar 2, which is more pathogenic than biovar 1, is also found. molecular methods for subtyping isolates are necessary for allowing epidemiological surveillance and control of eradication programs. due to the genetic homogeneity of the genus brucella, the development of molecular typing ... | 2014 | 24401776 |
| a mouse model of anemia of inflammation: complex pathogenesis with partial dependence on hepcidin. | anemia is a common complication of infections and inflammatory diseases, but the few mouse models of this condition are not well characterized. we analyzed in detail the pathogenesis of anemia induced by an injection of heat-killed brucella abortus and examined the contribution of hepcidin by comparing wild-type (wt) to iron-depleted hepcidin-1 knockout (hamp-ko) mice. b abortus-treated wt mice developed severe anemia with a hemoglobin nadir at 14 days and partial recovery by 28 days. after an e ... | 2014 | 24357728 |
| variables associated with infections of cattle by brucella abortus., leptospira spp. and neospora spp. in amazon region in brazil. | the frequency of neospora spp., leptospira spp. and brucella abortus infections in adult cattle was determined in herds of the state of pará, brazil, which is an important region for cattle production located in the amazon region. a total of 3466 adult female cattle from 176 herds were tested, leading to a frequency of seropositive animals of 14.7%, 3.7% and 65.5% and a herd positivity of 87.4%, 41.3% and 98.8% for infections caused by neospora spp., b. abortus and leptospira spp., respectively. ... | 2015 | 26302373 |
| structural basis for the inhibition of human lysozyme by plic from brucella abortus. | lysozymes are the first line of defense for a diverse range of organisms that catalyze the degradation of bacterial peptidoglycan. gram-negative bacteria produce proteinaceous lysozyme inhibitors to protect themselves from the action of lysozymes. to date, mlic or plic (membrane-bound or periplasmic inhibitor of c-type lysozyme, respectively) has been found in various gram-negative bacteria. here, we report the crystal structures of brucella abortus plic and its complex with human c-type lysozym ... | 2013 | 24308818 |
| urocanate as a potential signaling molecule for bacterial recognition of eukaryotic hosts. | host recognition is the crucial first step in infectious disease pathogenesis. recognition allows pathogenic bacteria to identify suitable niches and deploy appropriate phenotypes for successful colonization and immune evasion. however, the mechanisms underlying host recognition remain largely unknown. mounting evidence suggests that urocanate-an intermediate of the histidine degradation pathway-accumulates in tissues, such as skin, and acts as a molecule that promotes bacterial infection via mo ... | 2014 | 24305948 |
| immunogenic response induced by wzm and wzt gene deletion mutants from brucella abortus s19. | brucellosis is an infectious disease affecting humans and animals worldwide. effective methods of control include inducing immunity in animals by vaccination and elimination. brucella abortus s19 is one of the popular vaccines for control of cattle brucellosis, as it has low virulence. in this paper, allelic exchange plasmids of wzm and wzt genes were constructed and partially knocked out to evaluate the effects on the induction of immunity to brucella abortus s19 mutants. cytokine secretion in ... | 2014 | 24247358 |
| serological survey and risk factors for brucellosis in water buffaloes in the state of pará, brazil. | to evaluate the prevalence and possible risk factors for brucellosis caused by brucella abortus in water buffaloes in the state of pará, brazil, 3,917 female buffalo serum samples from pregnant and non-pregnant animals were examined: 2,809 from marajó island and 1,108 from the mainland. the buffered acidified plate antigen (bapa) screening test positively diagnosed 4.8% (188/3,917) of the animals with brucellosis, and the 2-mercaptoethanol (2-me) confirmatory test affirmed 95.7% (180/188) of the ... | 2014 | 24221991 |
| characterization of betaine aldehyde dehydrogenase (betb) as an essential virulence factor of brucella abortus. | the pathogenic mechanisms of brucellosis used to adapt to the harsh intracellular environment of the host cell are not fully understood. the present study investigated the in vitro and in vivo characteristics of b. abortus betaine aldehyde dehydrogenase (betb) (gene bank id: 006932) using a betb deletion mutant constructed from virulent b. abortus 544. in test under stress conditions, including osmotic- and acid stress-resistance, the betb mutant had a lower osmotic-resistance than b. abortus wi ... | 2014 | 24210811 |
| infection of c57bl/6 mice by trypanosoma musculi modulates host immune responses during brucella abortus cocolonization. | brucellosis, which results in fetal abortions in domestic and wildlife animal populations, is of major concern in the us and throughout much of the world. the disease, caused by brucella abortus, poses an economic threat to agriculture-based communities. a moderately efficacious live attenuated vaccine (b. abortus strain rb51) exists. however, even with vaccine use, outbreaks occur. evidence suggests that elk (cervus canadensis), a wild host reservoir, are the source of recent outbreaks in domes ... | 2014 | 24171573 |
| detection of volatile organic compounds in brucella abortus-seropositive bison. | brucellosis is of great public health and economic importance worldwide. detection of brucellosis currently relies on serologic testing of an antibody response to brucella infection, which suffers from cross-sensitivities to other antibody responses. here we present a new method for identifying brucella exposure that is based on profiling volatile organic compounds (vocs) in exhaled breath. breath samples from brucella-seropositive bison and controls were chemically analyzed and demonstrated sta ... | 2013 | 24156543 |
| brucella abortus induces collagen deposition and mmp-9 down-modulation in hepatic stellate cells via tgf-β1 production. | in patients with active brucellosis, the liver is frequently affected by histopathologic lesions, such as granulomas, inflammatory infiltrations, and parenchymal necrosis. herein, we examine some potential mechanisms of liver damage in brucellosis. we demonstrate that brucella abortus infection inhibits matrix metalloproteinase-9 (mmp-9) secretion and induces collagen deposition and tissue inhibitor of matrix metalloproteinase-1 secretion induced by hepatic stellate cells (lx-2). these phenomena ... | 2013 | 24113459 |
| serologic detection of antibodies to brucella spp. using a commercial elisa in cattle in grenada, west indies. | bovine brucellosis, caused mainly by brucella abortus, a zoonotic bacterium, has been reported from many areas of the world, including central and south america, and the caribbean island state of trinidad and tobago. although brucellosis has been eradicated from domestic cattle in canada it still exists in one or two herds in the united states. serological tests are important in estimating prevalence of brucella exposure in order to target eradication programmes. in this study, serum samples fro ... | 2013 | 23959493 |
| fixl-like sensor flbs of brucella abortus binds haem and is necessary for survival within eukaryotic cells. | replication of brucella inside eukaryotic cells is essential for pathogenesis, and successful infection requires rapid adaptation to the intracellular milieu. close relatives of brucella use the two-component system fixlj to survive inside the host. we aimed to identify a homologous sensor in brucella abortus. a predicted protein with transmembrane and conserved histidine kinase domains was identified as the fix-like brucella sensor, flbs. although it lacks the pas domain, recombinant flbs binds ... | 2013 | 23954290 |
| abortion and premature birth in cattle following vaccination with brucella abortus strain rb51. | brucella abortus rb51 is the vaccine strain currently licensed for immunizing cattle against brucellosis in the united states. most cattle are vaccinated as heifer calves at 4-12 months of age. adult cattle may be vaccinated in selected high-risk situations. two herds of pregnant adult cattle in the brucellosis-endemic area of wyoming were vaccinated with a standard label dose (1.0-3.4 × 10(10) organisms) of rb51. reproductive losses in the vaccinated herds were 5.3% (herd a) and 0.6% (herd b) a ... | 2013 | 23942901 |
| genetic stability of brucella abortus s19 and rb51 vaccine strains by multiple locus variable number tandem repeat analysis (mlva16). | the aims of the present study were (i) to assess the in vitro genetic stability of s19 and rb51 brucella abortus vaccines strains and (ii) to evaluate the ability of multiple-locus variable-number tandem repeat (vntr) analysis (mlva) as a tool to be used in the quality control of live vaccines against brucellosis. sixty-three batches of commercial s19 (n=53) and rb51 (n=10) vaccines, produced between 2006 and 2009, were used in this study. s19 and rb51 vaccines were obtained from, respectively, ... | 2013 | 23933375 |
| comparative brucella abortus antibody prevalence in cattle under contrasting husbandry practices in uganda. | a study was conducted in the luwero and nakasongola districts in central uganda to determine and compare the prevalence and distribution of antibodies against brucella abortus in cattle under contrasting husbandry practices, using two serological tests. three hundred and fifteen serum samples were systematically sampled from 29 farms and subsequently tested using the rose bengal plate test (rbpt) and indirect antibody enzyme linked immunosorbent assay (i-elisa). the overall prevalence of antibod ... | 2013 | 23905210 |
| isolation and identification of bovine brucella isolates from pakistan by biochemical tests and pcr. | brucellosis is endemic in bovines in pakistan. the brucella species and biovars involved, however, are unknown. the objectives of the present study were to isolate and characterize brucellae from seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes which had recently aborted. the seropositive milk samples, aborted fetuses, and vaginal swabs of cattle and buffaloes were collected from the potohar plateau, pakistan. isolation of brucellae was done on modified farre ... | 2014 | 23868548 |
| protection to respiratory challenge of brucella abortus strain 2308 in the lung. | brucella is amongst the top 5 causes of zoonotic disease worldwide. infection is through ingestion, inhalation or contact exposure. brucella is characterized as a class b pathogen by centers of disease control and prevention (cdc). currently, there are no efficacious vaccines available in people. currently available usda approved vaccines for animals include b. abortus strain rb51 and b. melitensis rev1. protection is mediated by a strong innate and cd4 th1, cd8 tc1 immune response. if protectiv ... | 2013 | 23845817 |
| hidden behind autophagy: the unconventional roles of atg proteins. | macroautophagy (hereafter referred to as autophagy) is an evolutionarily conserved intracellular catabolic transport route that generally allows the lysosomal degradation of cytoplasmic components, including bulk cytosol, protein aggregates, damaged or superfluous organelles and invading microbes. target structures are sequestered by double-membrane vesicles called autophagosomes, which are formed through the concerted action of the autophagy (atg)-related proteins. until recently it was assumed ... | 2013 | 23837619 |
| integrating ecology with management to control wildlife brucellosis. | bison (bison bison) and elk (cervus elaphus) in the greater yellowstone ecosystem have long been infected with brucella abortus. the continued culling of large numbers of yellowstone bison to reduce the risk of brucellosis transmission to cattle could negatively affect long-term conservation. a desirable management objective is to reduce the level of b. abortus infection while conserving wildlife populations. identifying the ecological factors that influence immune suppression and vulnerabilityt ... | 2013 | 23837381 |
| the russian experience in brucellosis veterinary public health. | russia has extensive experience in controlling brucellosis in large and small animals, based on systematic animal health and disease control measures, combined with the use of specific prophylaxis. widespread application of the live brucellosis vaccine brucella abortus strain 82 has led to a dramatic reduction in the prevalence of brucellosis. among the distinctive properties of this vaccine are weak agglutinogenicity and high anti-epizootic efficacy, which allow differentiation between infected ... | 2013 | 23837380 |
| pathogenesis and pathobiology of zoonotic brucellosis in humans. | although human brucellosis has protean clinical manifestations, affected tissues usually exhibit signs of inflammation. the cellular and molecular bases of some immunopathological phenomena probably involved in the pathogenesis of infection with brucellae have been elucidated recently. human osteoblasts and fibroblast-like synoviocytes produce cytokines, chemokines and matrix metalloproteinases in response to infection with brucellae and/or to stimulation by brucellae-infected monocytes. in turn ... | 2013 | 23837370 |
| an ecological perspective on brucella abortus in the western united states. | after a hiatus during the 1990s, outbreaks of brucella abortus in cattle are occurring more frequently in some of the western states of the united states, namely, montana, wyoming and idaho. this increase is coincidentwith increasing brucellosis seroprevalence in elk (cervus elaphus), which is correlated with elk density. vaccines are a seductive solution, but their use in wildlife systems remains limited by logistical, financial, and scientific constraints. cattle vaccination is ongoing in the ... | 2013 | 23837367 |
| risks of brucella abortus spillover in the greater yellowstone area. | recurrent spillover of brucella abortus from wildlife reservoirs to domestic cattle in the greater yellowstone area (gya) has prevented the united states from completely eradicating bovine brucellosis. risks to cattle are a function of the size and location of wildlife and livestock populations, the degree and nature of spatio-temporal interactions between the various hosts, the level of disease in wildlife, and the susceptibility of livestock herds. while the brucellosis prevalence in wild, fre ... | 2013 | 23837366 |
| epidemiology of brucellosis in domestic animals caused by brucella melitensis, brucella suis and brucella abortus. | brucellosis is a disease that causes severe economic losses for livestock farms worldwide. brucella melitensis, b. abortus and b. suis, which are transmitted between animals both vertically and horizontally, cause abortion and infertility in their primary natural hosts - goats and sheep (b. melitensis), cows (b. abortus) and sows (b. suis). brucella spp. infect not only their preferred hosts but also other domestic and wild animal species, which in turn can act as reservoirs of the disease for o ... | 2013 | 23837364 |
| ¹³c, ¹⁵n and ¹h backbone and side chain chemical shift assignment of acid-stress bacterial chaperone hdea at ph 6. | hdea is a small chaperone found in the periplasm of several common pathogenic bacteria (escherichia coli, shigella flexneri and brucella abortus) which are the leading causes of dysentery worldwide, especially in developing countries. its job is to protect other periplasmic proteins from aggregating as the bacteria pass through the low ph environment of the human stomach on their way to infect the intestines. hdea is an inactive folded dimer at neutral ph, but becomes a disordered active monomer ... | 2014 | 23835624 |
| protective effect of a dna vaccine containing an open reading frame with homology to an abc-type transporter present in the genomic island 3 of brucella abortus in balb/c mice. | the immunogenicity of a dna vaccine containing an open reading frame (orf) of genomic island 3 (gi-3), specific for brucella abortus and brucella melitensis, has been examined. intramuscular injection of plasmid dna carrying the open reading frame with homology to an abc-type transporter (pv278a) into balb/c mice elicited both humoral and cellular immune responses. mice injected with pv278a had a dominant immunoglobulin g2a (igg2a) response. this dna vaccine elicited a t-cell-proliferative respo ... | 2013 | 23834811 |
| investigating genetic diversity of brucella abortus and brucella melitensis in italy with mlva-16. | despite the eradication of brucellosis from most of europe, the disease remains relatively common in a variety of livestock in southern european countries. it is therefore surprising that with such high prevalence rates, there have been few genetic characterizations of brucellosis outbreaks in this region. we conducted a genetic assessment of 206 isolates of brucella abortus and b. melitensis from italy using variable number tandem repeats (vntrs). we determined genetic diversity and geographic ... | 2013 | 23831636 |
| evaluation of bison (bison bison) semen from yellowstone national park, montana, usa, bulls for brucella abortus shedding. | to determine if bison (bison bison) bulls from yellowstone national park (ynp), montana, usa, shed an infective dose of brucella abortus in semen, 50 ynp bulls were captured on public lands in montana during the winter and early spring (april-may) of 2010 and 2011. the bulls were immobilized, and blood and semen samples were collected for serology and brucella culture. thirty-five bulls (70%) were antibody-positive, and b. abortus was cultured from semen in three (9%) of the 35 antibody-positive ... | 2013 | 23778628 |
| evaluation of transmission of brucella abortus strain 19 in bison by intravaginal, intrauterine, and intraconjunctival inoculation. | bovine brucellosis, caused by the bacterium brucella abortus, is endemic in bison (bison bison) and elk (cervus elaphus nelsoni) populations in the area of yellowstone national park, usa. two strategies have been proposed to reduce the risk of transmission of disease in bison: remote vaccination with the vaccine rb51, and the use of immunocontraception of bison to decrease shedding of organisms from infected females. the frequent occurrence of venereal transmission in bison would complicate eith ... | 2013 | 23778600 |
| brucellosis in humans--etiology, diagnostics, clinical forms. | brucellosis in humans is a zoonosis of greatly varied clinical image. it occurs on all inhabited continents. the course of the disease may be acute, sub-acute or chronic. the etiologic factors of brucellosis are small, aerobic gram-negative rods of the genus brucella, which currently contains ten species: b. abortus, b. suis, b. ovis, b. melitensis, b. canis, b. neotomae, b. pinnipedialis, b. ceti, b. microti and b. inopinata. in humans, the disease is caused mainly by: b. melitensis as the most ... | 2013 | 23772567 |
| detection of antibodies against brucella abortus, leptospira spp., and apicomplexa protozoa in water buffaloes in the northeast of argentina. | water buffalo industry has become a profitable activity worldwide, including the northeast of argentina (nea). however, research on diseases affecting this species is scarce. the aim of the present study was to detect antibodies against brucella abortus, leptospira spp., neospora caninum, toxoplasma gondii, and sarcocystis spp. in 500 water buffalo cows from five ranches (100 animals each) in the nea. serum samples were tested for b. abortus by fluorescence polarization assay, leptospira spp. by ... | 2013 | 23765549 |
| a duplex pcr for rapid and simultaneous detection of brucella spp. in human blood samples. | to design a duplex pcr for rapid and simultaneous detection of brucella species. in human blood samples. | 2013 | 23711705 |
| functional analysis of bovine nramp1 and production of transgenic cloned embryos in vitro. | natural resistance-associated macrophage protein 1 (nramp1) plays an important role in restraining the growth of intracellular pathogens within macrophages. in this study, nramp1 cdna was cloned from qinchuan cattle and its anti-bacterial activity was demonstrated as being able to significantly inhibit the growth of salmonella abortusovis and brucella abortus in macrophages. calf fibroblasts stably transfected with psp-nramp1-ha vector were used to reconstruct bovine embryos by somatic cell nucl ... | 2015 | 23683995 |
| evaluation of brucella abortus s19 vaccines commercialized in brazil: immunogenicity, residual virulence and mlva15 genotyping. | live attenuated brucella abortus s19 is the most effective vaccine against brucellosis in cattle. the assessment of the immunological parameters is essential to guarantee the biological quality of live anti-bacteria vaccines. the evaluation of genetic stability of live bacterial vaccines is also important in quality control. the aims of the present study were to compare (i) the immunogenicity and residual virulence, and (ii) the genotypic profile (mlva15) of the eight s19 vaccines commercialized ... | 2013 | 23664986 |
| male rats transmit brucella abortus biotype 1 through sexual intercourse. | the aim of this study was to evaluate transmission of brucella abortus biotype 1 via sexual intercourse in rats. male and female virgin sprague-dawley (sd) rats were experimentally infected intraperitoneally with 1×10(9)colony forming units (cfu) of b. abortus biotype 1, a korean bovine isolate. at 14 days after infection, infected male rats (n=10) were housed with uninfected female rats (n=10) and infected female rats (n=10) were housed with uninfected male rats (n=10) for a period of one month ... | 2013 | 23664185 |
| the ferrous iron transporter ftrabcd is required for the virulence of brucella abortus 2308 in mice. | iron transport has been linked to the virulence of brucella strains in both natural and experimental hosts. the genes designated bab2_0837-0840 in the brucella abortus 2308 genome sequence are predicted to encode a cupii-type ferrous iron transporter homologous to the ftrabcd transporter recently described in bordetella. to study the role of the brucella ftrabcd in iron transport, an isogenic ftra mutant was constructed from b. abortus 2308. compared with the parental strain, the b. abortus ftra ... | 2013 | 23647104 |
| [establishment and preliminary application of a quantitative real-time pcr method for detecting the transcriptions of th1/th2 cytokines in raw264.7 cells]. | to establish a quantitative real-time pcr method to detect the transcriptions of th1/th2 cytokines in raw264.7 cells. | 2013 | 23643175 |
| molecular strain typing of brucella abortus isolates from italy by two vntr allele sizing technologies. | brucellosis, one of the most important re-emerging zoonoses in many countries, is caused by bacteria belonging to the genus brucella. furthermore these bacteria represent potential biological warfare agents and the identification of species and biovars of field strains may be crucial for tracing back source of infection, allowing to discriminate naturally occurring outbreaks instead of bioterrorist events. in the last years, multiple-locus variable-number tandem repeat analysis (mlva) has been p ... | 2013 | 23585050 |
| epidemiologic relatedness between brucella abortus isolates from livestock and wildlife in south korea. | to investigate the epidemiologic relatedness of brucella abortus isolates from chinese water deer (hydropotes inermis) and goral (naemorhedus goral raddeanus) in 2010-2011, 22l isolates from livestock (including domestic elk, cervus canadensis) were analyzed using the multilocus variable-number tandem repeats analysis. in the clustering analysis, korean b. abortus isolates were divided into 40 genotypes by 18 markers, and 2 b. abortus isolates from wildlife were clustered with those of domestic ... | 2013 | 23568927 |
| use of serology and bacterial culture to determine prevalence of brucella spp. in feral swine (sus scrofa) in proximity to a beef cattle herd positive for brucella suis and brucella abortus. | using serology and bacterial culture, we determined the prevalence of brucella spp. and the antibody to brucella spp. in a feral swine (sus scrofa) population in proximity to a cattle herd that was culture positive for brucella abortus and brucella suis in north-central texas, usa. during a prospective cross-sectional quantitative study in april 2005, we collected blood and tissue samples from 40 feral swine within a 30-km radius of the infected herd. serum samples were tested by the rose bengal ... | 2013 | 23568896 |
| seroprevalence and risk factors associated with brucellosis as a professional hazard in pakistan. | the present study was conducted to determine the seroprevalence and identify risk factors associated with brucellosis in humans at high risk in the potohar plateau of northeastern pakistan. a total of 262 serum samples were collected from persons of different occupational groups: veterinary personnel, milkers, abattoir workers, livestock farmers, and others (drivers, security guards, housewives). data related to gender, age, occupation, contact with animals, brucellosis-related symptoms, consump ... | 2013 | 23560424 |
| the two-component systems prrba and ntryx co-ordinately regulate the adaptation of brucella abortus to an oxygen-limited environment. | brucella is the causative agent of the zoonotic disease brucellosis, which is endemic in many parts of the world. the success of brucella as pathogen relies in its ability to adapt to the harsh environmental conditions found in mammalian hosts. one of its main adaptations is the induction of the expression of different genes involved in respiration at low oxygen tension. in this report we describe a regulatory network involved in this adaptation. we show that brucella abortus prrba is a function ... | 2013 | 23527685 |
| inactivation of the abc transporter atpase gene in brucella abortus strain 2308 attenuated the virulence of the bacteria. | brucella abortus is a gram-negative, facultative intracellular bacterial pathogen of human and other animals. brucella lipopolysaccharide has been identified as an important virulence factor. in this study, the abc transporter atpase gene (bab1_0542) of b. abortus strain s2308 was inactivated by deleting a 446-bp fragment from the gene, thereby generating the mutant strain, s2308δatp. real time pcr analysis confirmed the inactivation of this gene with no polar effect on the transcription of adja ... | 2013 | 23507429 |
| serologic survey of infectious diseases in populations of maned wolf (chrysocyon brachyurus) and crab-eating fox (cerdocyon thous) from aguas emendadas ecological station, brazil. | domestic dogs are reservoirs for many infectious diseases and may represent a potential source of infection for wild canid populations. a serologic investigation of antibodies to toxoplasma gondii, neospora caninum, brucella abortus, and leptospira spp. was conducted on three maned wolves (chrysocyon brachyurus) and seven crab-eating foxes (cerdocyon thous), all free-living, at the aguas emendadas ecological station (esecae), federal district, brazil, between february and october 2006. out of th ... | 2013 | 23505716 |
| importance of identification and typing of brucellae from west african cattle: a review. | bovine brucellosis is an endemic infectious disease which can impact cattle productivity and welfare negatively, as well as human health. sufficient knowledge on its epidemiology, particularly on species and biotypes of brucella at national and/or regional scale are important to set up and implement efficient control measures against brucellosis in a "one health" perspective. the main objective of this review was to investigate available literature on strains of brucella in order to provide a st ... | 2013 | 23499188 |
| critical role of asc inflammasomes and bacterial type iv secretion system in caspase-1 activation and host innate resistance to brucella abortus infection. | pathogens are detected by innate immune receptors that, upon activation, orchestrate an appropriate immune response. recent studies revealed the intracellular signaling cascades involved in the tlr-initiated immune response to brucella abortus infection. however, no report has elucidated the role of inflammasome receptors in brucella recognition. therefore, we decided to investigate the function of nlrc4, nlrp3, and aim2 in sensing brucella. in this study, we showed that nlrc4 is not required to ... | 2013 | 23460746 |
| evaluation of four dna extraction protocols for brucella abortus detection by pcr in tissues from experimentally infected cows with the 2308 strain. | this study compared 4 protocols for dna extraction from homogenates of 6 different organs of cows infected with the brucella abortus 2308 strain. the extraction protocols compared were as follows: gt (guanidine isothiocyanate lysis), boom (gt lysis with the carrying suspension diatomaceous earth), pk (proteinase k lysis), and santos (lysis by boiling and freezing with liquid nitrogen). positive and negative gold standard reference groups were generated by classical bacteriological methods. all s ... | 2013 | 23421881 |
| natural is711 insertion causing omp31 gene inactivation in brucella ovis. | the present report describes an atypical brucella ovis strain (bo10) isolated from the epididymis and testis of an infected ram. macroscopic and microscopic lesions characteristic for the infection, including positive brucella immunostaining, were observed within lesions in the genital organs. compared to other isolates, strain bo10 required an additional day (a total of 96 hr) of incubation to form visible colonies, showed a distinct carbon source utilization profile, agglutinated only weakly w ... | 2013 | 23404477 |
| sequential real-time pcr assays applied to identification of genomic signatures in formalin-fixed paraffin-embedded tissues: a case report about brucella-induced osteomyelitis. | brucellosis is a zoonotic infection transmitted from animals to human by ingestion of infected food products, direct contact with an infected animal, or inhalation of aerosols. brucella infection-induced osteomyelitis may present only with nonspecific clinical and radiographic findings, mild elevations in serum inflammatory markers, as well as nonspecific histological changes. we studied a case of an iraqi war veteran with multifocal vertebral body and left iliac bone lesions on radio nucleotide ... | 2013 | 23356125 |
| tandem repeats of the extracellular domain of matrix 2 influenza protein exposed in brucella lumazine synthase decameric carrier molecule induce protection in mice. | the antigenic variation of influenza virus represents a major prevention problem. however, the ectodomain of the protein matrix 2 (m2e) is nearly invariant in all human influenza a strains and has been considered as a promising candidate for a broadly protective vaccine because antibodies to m2e are protective in animal models. in this work we evaluated the possible use of brucella abortus lumazine synthase protein (bls), a highly immunogenic decameric protein, as a carrier of the m2e peptide. c ... | 2013 | 23246552 |
| lipopolysaccharide as a target for brucellosis vaccine design. | the gram-negative bacteria of the genus brucella are facultative intracellular parasites that cause brucellosis, a world wide-distributed zoonotic disease that represents a serious problem for animal and human health. there is no human-to-human contagion and, since there is no human vaccine, animal vaccination is essential to control brucellosis. however, current vaccines (all developed empirically) do not provide 100% protection and are infectious in humans. attempts to generate new vaccines by ... | 2013 | 23219811 |
| renal cyst infection caused by brucella abortus. | a 49-year-old man with a medical history of polycystic kidney disease was presented to the emergency department with fever and left flank pain. abdominal examination revealed an enlarged and painful left kidney. the c-reactive protein level was significantly high and the magnetic resonance imaging revealed areas of abnormal intensity and fluid-fluid levels in renal cysts. brucella abortus was yielded from both blood and cyst fluid culture. standard therapy (rifampicin plus doxycycline) of brucel ... | 2013 | 23176669 |
| small gtpases and brucella entry into the endoplasmic reticulum. | a key determinant for intracellular pathogenic bacteria to ensure their virulence within host cells is their ability to bypass the endocytic pathway and to reach a safe niche of replication. in the case of brucella, the bacterium targets the er (endoplasmic reticulum) to create a replicating niche called the bcv (brucella-containing vacuole). the er is a suitable strategic place for pathogenic brucella. indeed, bacteria can be hidden from host cell defences to persist within the host, and they c ... | 2012 | 23176479 |
| phellinus baumii extract influences pathogenesis of brucella abortus in phagocyte by disrupting the phagocytic and intracellular trafficking pathway. | to clarify the effects of phellinus baumii ethanol extract (pbe) on brucella abortus pathogenesis in phagocytes focusing on the phagocytic and intracellular trafficking pathway. | 2013 | 23163237 |
| retraction: incidence study of brucella abortus and brucella melitensis in bovine and buffalo semen samples by real-time pcr assay in iran. | this article released online on august 3, 2012 as advance publication was withdrawn from consideration for publication in the journal of veterinary medical science at author's request. | 2012 | 23123884 |
| redundant effects of ketamine on the pathogenesis and severity of brucella abortus infection. | brucella abortus is an intracellular bacterium and leading to a serious debilitating disease known as brucellosis. ketamine is an anesthetic and a sedative that affects the immunomodulatory activities of various immune cells. the current study was to elucidate the role of ketamine in b. abortus infection, focusing on the phagocytic activity and immune response of macrophages. following incubation of murine macrophages with ketamine, the phagocytosis of b. abortus was markedly reduced compared wi ... | 2013 | 23107514 |
| brucella abortus induces intracellular retention of mhc-i molecules in human macrophages down-modulating cytotoxic cd8(+) t cell responses. | brucella abortus elicits a vigorous th1 immune response which activates cytotoxic t lymphocytes. however, b. abortus persists in its hosts in the presence of cd8(+) t cells, establishing a chronic infection. here, we report that b. abortus infection of human monocytes/macrophages inhibited the ifn-γ-induced mhc-i cell surface expression. this phenomenon was dependent on metabolically active viable bacteria. mhc-i down-modulation correlated with the development of diminished cd8(+) cytotoxic t ce ... | 2013 | 23107169 |
| genetic analysis of the 3' untranslated region of the bovine slc11a1 gene reveals novel polymorphisms. | polymorphisms in microsatellites at the 3' untranslated region (3'utr) of the slc11a1 (solute carrier family 11 member a1) gene have been associated with natural resistance to brucella abortus and mycobacterium bovis infection in livestock species. here, we carried out an individual genetic analysis of the two microsatellites present at the 3'utr slc11a1 gene in 254 bos taurus purebred, 125 b. indicus purebred and 54 b. taurus × b. indicus crossbred cattle. the genotyping by capillary electropho ... | 2013 | 23065223 |
| bovine brucellosis in argentina and bordering countries: update. | bovine brucellosis is a zoonotic disease spread worldwide. the infection in cattle is predominantly caused by brucella abortus and is usually detected in pregnant females through abortions. the disease is endemic in argentina; however, infection in humans is underestimated and often not reported. the prevalence of bovine brucellosis in countries bordering argentina is quite variable: 0.04% in uruguay, 10.20% in the north and 0.06% in the south of brazil, 0.2% in chile, 3.15% in paraguay and 2.27 ... | 2014 | 23046031 |
| evaluation of protective effect of dna vaccines encoding the bab1_0263 and bab1_0278 open reading frames of brucella abortus in balb/c mice. | the immunogenicity of two dna vaccines encoding open reading frames (orfs) of genomic island 3 (gi-3), specific for brucella abortus and brucella melitensis, has been examined. intramuscular injection of plasmid dna carrying the bab1_0263 and bab1_0278 genes (pvf263 and pvf278, respectively) into balb/c mice elicited both humoral and cellular immune responses. mice injected with pvf263 or pvf278 had a dominant immunoglobulin g2a (igg2a) response. in addition, both dna vaccines elicited a t-cell- ... | 2012 | 23026687 |
| the role of a brucella abortus lipoprotein in intracellular replication and pathogenicity in experimentally infected mice. | brucella abortus, the causative agent of brucellosis, can survive and replicate within host cells. understanding bacterial virulence factors and bacteria-host cell interactions is critical for controlling brucellosis. however, little is known regarding the pathogenic mechanisms of brucellosis. a lipoprotein mutant (gene bank id: 3339351) of b. abortus showed a lower rate of intracellular replication than did the wild-type strain in hela cells and raw 264.7 macrophages. the adherent activity of t ... | 2013 | 23006628 |
| molecular insights into bacteroid development during rhizobium-legume symbiosis. | rhizobial soil bacteria can form a symbiosis with legumes in which the bacteria fix atmospheric nitrogen into ammonia that can be utilized by the host. the plant, in turn, supplies the rhizobia with a carbon source. after infecting the host cell, the bacteria differentiate into a distinct bacteroid form, which is able to fix nitrogen. the bacterial baca protein is essential for bacteroid differentiation in legumes producing nodule-specific cysteine-rich peptides (ncrs), which induce the terminal ... | 2013 | 22998605 |
| isolation of a field strain of brucella abortus from rb51-vaccinated- and brucellosis-seronegative bovine yearlings that calved normally. | a study was carried out in pichucalco, chiapas (mexico) to determine whether recently calved cows or those that aborted shed brucella. serological diagnosis of brucellosis was made in all animals (209). six of the cows that calved normally and two that aborted underwent a bacteriological study of milk and vaginal exudate. brucella abortus was isolated from vaginal exudate samples in two 3- to 4-year-old seronegative first-birth cows that had calved normally. this was confirmed through bacteriolo ... | 2013 | 22956439 |
| short communication: evaluation of brucella infection of cows by pcr detection of brucella dna in raw milk. | the collection of serum samples from cows is frequently difficult to implement in large-scale surveys, and may involve a high risk of occupational infection. this study assessed the potential of using raw milk sampling as a suitable method for use in a pilot survey of brucella abortus infection status in cattle. raw milk samples from 816 cows were examined. polymerase chain reaction assays of raw milk, with primers derived from the is711 element of the brucella genome, were used. of the cows, 55 ... | 2012 | 22916890 |
| brucella abortus-infected macrophages modulate t lymphocytes to promote osteoclastogenesis via il-17. | the pathogenic mechanisms of bone loss caused by brucella species have not been completely deciphered. although t lymphocytes (lts) are considered important to control infection, the mechanism of brucella-induced t-cell responses to immunopathological features is not known. we present in vitro and in vivo evidence showing that brucella abortus-induced inflammatory response leads to the activation of lts, which further promote osteoclastogenesis. pre-activated murine lts treated with culture supe ... | 2012 | 22901753 |
| characterization of brucella abortus infection of bovine monocyte-derived dendritic cells. | brucella abortus is a gram negative facultative intracellular pathogen of cattle, and an important zoonosis in humans worldwide. previous studies have shown that dendritic cells (dc) from humans and mice are highly permissive for brucella survival and proliferation. impairment of dc activation and maturation by brucella infection has also been reported in these two species. the aim of this study was to characterize infection of bovine dc with b. abortus. monocyte-derived dc (mddc) were cultured ... | 2012 | 22884262 |
| the narrow active-site cleft of o-acetylserine sulfhydrylase from leishmania donovani allows complex formation with serine acetyltransferases with a range of c-terminal sequences. | cysteine is a crucial substrate for the synthesis of glutathione and trypanothione, which in turn maintain intracellular redox homeostasis and defend against oxidative stress in the pathogen leishmania donovani. here, the identification, sequencing, characterization and crystal structure at 1.79 å resolution of o-acetylserine sulfhydrylase (oass), a cysteine-biosynthetic pathway enzyme from l. donovani (ldoass), are reported. it shows binding to the serine acetyltransferase (sat) c-terminal pept ... | 2012 | 22868756 |
| withdrawn: incidence study of brucella abortus and brucella melitensis in bovine and buffalo semen samples by real-time pcr assay in iran. | ahead of print article withdrawn by publisher. | 2012 | 22864410 |
| vaccination with recombinant flagellar proteins flgj and flin induce protection against brucella abortus 544 infection in balb/c mice. | brucella has been considered as a non-motile, facultative intracellular pathogenic bacterium. however, the genome sequences of different brucella species reveal the presence of the flagellar genes needed for the construction of a functional flagellum. due to its roles in the interaction between pathogen and host, we hypothesized that some of the flagellar proteins might induce protective immune responses and these proteins will be good subunit vaccine candidates. this study was conducted to scre ... | 2012 | 22854331 |
| a brucella abortus csta mutant is defective for association with endoplasmic reticulum exit sites and displays altered trafficking in hela cells. | members of the genus brucella are facultative intracellular pathogenic bacteria able to control maturation of their vacuoles. in several cell types, brucella is able to reach a proliferation compartment derived from the endoplasmic reticulum (er). since er exit site (eres) functions are required for brucella proliferation, we performed a yeast two-hybrid screen between human eres-associated proteins and the predicted brucella proteome. this screening led to the identification of csta, a conserve ... | 2012 | 22820839 |
| immunogenicity and protective potential of a bacterially expressed recombinant dihydrolipoamide succinyltransferase (re2o) of brucella abortus in balb/c mice. | brucellosis is one of the world's major zoonoses. no vaccine is available for the prevention of brucellosis in human. efforts are needed to develop an effective, safe, stable, vaccine with long lasting immunity against human brucellosis. here, we cloned and expressed recombinant dihydrolipoamide succinyltransferase (re2o) of brucella abortus in escherichia coli and purified up to homogeneity by metal affinity chromatography. the purified re2o is immunoreactive with brucellosis positive cattle se ... | 2012 | 22806154 |
| effect of exogenous erythritol on growth and survival of brucella. | erythritol has been considered as an important factor for the pathogenesis of brucella abortus 2308 and its ability to cause abortion in ruminants. there is a lack of laboratory models to study the brucella-erythritol relationship, as commonly used murine models do not have erythritol. we tested the effect of exogenous erythritol on the growth of brucella in iron minimal medium (imm), in infected macrophage culture and in infected mice to determine if these models can be used to study the relati ... | 2012 | 22784921 |
| serologic survey of brucellosis in captive neotropical wild carnivores in northeast brazil. | abstract. this study reports the detection of antibodies against brucella abortus and b. canis in wild neotropical carnivores kept in captivity in three zoos in northeastern brazil. a total of 42 serum samples were examined, 17 from coatis (nasua nasua), eight from crab-eating raccoons (procyon cancrivorus), three from crab-eating foxes (cerdocyon thous), three from hoary foxes (lycalopex vetulus), two from little spotted cats (leopardus tigrinus), five from tayras (eira barbara), two from great ... | 2012 | 22779245 |
| cutting edge: brucella abortus exploits a cellular prion protein on intestinal m cells as an invasive receptor. | brucella abortus is a gram-negative bacterium causing brucellosis. although b. abortus is known to infect via the oral route, the entry site in the gastrointestinal tract has been unclear. we found that b. abortus was selectively internalized by microfold cells (m cells), a subset of epithelial cells specialized for mucosal ag uptake. during this process, colocalization of cellular prion protein (prp(c)) and b. abortus was evident on the apical surface as well as in subapical vacuolar structures ... | 2012 | 22772447 |
| yersinia enterocolitica: an unlikely cause of positive brucellosis tests in greater yellowstone ecosystem bison (bison bison). | yersinia enterocolitica serotype o:9 has identical o-antigens to those of brucella abortus and has apparently caused false-positive reactions in numerous brucellosis serologic tests in elk (cervus canadensis) from southwest montana. we investigated whether a similar phenomenon was occurring in brucellosis antibody-positive bison (bison bison) using y. enterocolitica culturing techniques and multiplex pcr of four diagnostic loci. feces from 53 yellowstone bison culled from the population and 113 ... | 2012 | 22740518 |
| recombinant virb5 protein as a potential serological marker for the diagnosis of bovine brucellosis. | the molecular tag vaccine against brucella abortus and serological testing are the main methods of prevention of brucellosis used currently. they can discriminate vaccinated animals and humans from those naturally infected. in this study, we constructed a gene deletion mutant strain, b. abortus s19 virb5 with a molecular tag. recombinant virb5 was expressed and purified for evaluation as a diagnostic reagent for bovine brucellosis. in total, 400 sera samples were tested using a virb5 antigen-bas ... | 2012 | 22662340 |
| redox-responsive regulation of denitrification genes in brucella. | brucella strains encounter oxygen deprivation during their intracellular replication in host cells, and the capacity of these bacteria to utilize no(3) as an alternative electron acceptor for respiration plays an important role in their successful adaption to their intracellular niche. in this issue of molecular microbiology, carrica et al (2012). report that ntry and ntrx comprise a redox-responsive two-component regulator in brucella abortus 2308 that responds to decreasing levels of o(2) and ... | 2012 | 22582958 |
| the ntry/x two-component system of brucella spp. acts as a redox sensor and regulates the expression of nitrogen respiration enzymes. | brucella spp. are facultative intracellular bacteria pathogenic for many mammalian species including humans, causing a disease called brucellosis. learning how brucella adapts to its intracellular niche is crucial for understanding its pathogenesis mechanism, allowing for the development of new and more effective vaccines and treatments against brucellosis. brucella pathogenesis resides mostly in its ability to adapt to the harsh environmental conditions encountered during host infection such as ... | 2012 | 22582926 |
| immunoproteomics of brucella abortus reveals differential antibody profiles between s19-vaccinated and naturally infected cattle. | brucella abortus is a gram-negative intracellular bacterium that causes infectious abortion in food-producing animals and chronic infection in humans. this study aimed to characterize a b. abortus s19 antigen preparation obtained by triton x-114 (tx-114) extraction through immunoproteomics to differentiate infected from vaccinated cattle. three groups of bovine sera were studied: gi, 30 naturally infected cows; gii, 30 s19-vaccinated heifers; and giii, 30 nonvaccinated seronegative cows. one-dim ... | 2012 | 22539433 |
| in vitro activities of antimicrobials against brucella abortus isolates from cattle in korea during 1998-2006. | in vitro activities of 13 antibiotics were assessed against 85 brucella abortus isolates from naturally infected cattle in the republic of korea during 1998-2006, using broth microdilution test. tetracyclines showed the most excellent activity against b. abortus, displaying mic values of 0.5 μg/ml or below. in particular, minocycline showed the lowest mic₅₀/₉₀ values (0.125/0.125 μg/ml) in this study. among four fluoroquinolones tested, ciprofloxacin (mic₅₀/₉₀, 0.5/1 μg/ml) and norfloxacin (mic₅ ... | 2012 | 22534307 |
| the mgla gene and its flanking regions in brucella: the role of mgla in tolerance to hostile environments, fe-metabolism and in vivo persistence. | we previously demonstrated that a spontaneous smooth small-colony variant of brucella abortus s19 is characterized by increased in vivo persistence and the differential expression of a gene predicted to encode a galactoside transport atp binding protein (mgla). in order to further investigate the role of this gene in the context of its flanking regions, we analyzed the respective dna sequences from the formerly described b. abortus s19 as well as from avirulent b. neotomae 5k33 and compared thes ... | 2012 | 22534190 |