| defining the kinetics, phenotype, and function of t cells induced by mycobacterium tuberculosis: pillar of immunity to tuberculosis. | | 2017 | 28739592 |
| loculated empyema due to tuberculosis in a child. | a 9-year-old girl from black ethnic origin presented with a history of fever, cough, loss of weight and right-sided chest wall pain for 2 weeks. chest x-ray demonstrated an effusion, which was shown to be loculated on chest ct scan. she was not responding to medical treatment and at thoracotomy loculated pus was drained. mycobacterium tuberculosis (tb) was cultured from the pus. tb is a rare cause of loculated empyema with an overlapping clinical and radiological picture with pyogenic infections ... | 2017 | 28739566 |
| xpert® mtb/rif: usefulness for the diagnosis of tuberculosis and resistance to rifampicin. | the advent of the xpert® mtb/rif technique was a revolution in the diagnosis of tuberculosis, especially in areas with high incidence and low resources. it allows the detection of mycobacterium tuberculosis complex and simultaneously the most common resistance mutations to rifampicin in less than 2h. for respiratory samples the sensitivity is very high, but it decreases for extrapulmonary samples and children. although it is faster and simpler than conventional methods, it presents some limitati ... | 2017 | 28739268 |
| optimization and interpretation of serial quantiferon testing to measure acquisition of m. tuberculosis infection. | conversion from a negative to positive quantiferon-tb test is indicative of mycobacterium tuberculosis (m.tb) infection, which predisposes to tuberculosis disease. interpretation of serial tests is confounded by immunological and technical variability. | 2017 | 28737960 |
| unveiling the catalytic role of b-block histidine in the n-s acyl shift step of protein splicing. | protein splicing is a post-translational modification that involves the excision of a segment denoted as "intein" and the joining of its two flanking segments. the process is autocatalytic, making inteins appealing for many applications in biotechnology, bioengineering, or medicine. the canonical mechanism of protein splicing is composed of four sequential steps, and is initialized by an n-s or n-o acyl shift to form a linear ester. it is well-established that a histidine, the most conserved ami ... | 2017 | 28737941 |
| heterogeneity in tuberculosis. | infection with mycobacterium tuberculosis, the causative agent of tuberculosis (tb), results in a range of clinical presentations in humans. most infections manifest as a clinically asymptomatic, contained state that is termed latent tb infection (ltbi); a smaller subset of infected individuals present with symptomatic, active tb. within these two seemingly binary states, there is a spectrum of host outcomes that have varying symptoms, microbiologies, immune responses and pathologies. recently, ... | 2017 | 28736436 |
| comparative genomic analysis of mycobacterium tuberculosis beijing-like strains revealed specific genetic variations associated with virulence and drug resistance. | isolates of the mycobacterium tuberculosis lineage 2/east-asian are considered one of the most successful strains due to their increased pathogenicity, hyper-virulence associated with drug resistance, and high transmission. recent studies in colombia have shown that the beijing-like genotype is associated with multidrug-resistance and high prevalence in the southwest of the country, but the genetic basis of its success in dissemination is unknown. in contribution to this matter, we obtained the ... | 2017 | 28734764 |
| tuberculosis in children. | mycobacterium tuberculosis is the leading cause of death worldwide from a single bacterial pathogen. the world health organization estimates that annually 1 million children have tuberculosis (tb) disease and many more harbor a latent form. accurate estimates are hindered by under-recognition and challenges in diagnosis. to date, an accurate diagnostic test to confirm tb in children does not exist. treatment is lengthy but outcomes are generally favorable with timely initiation. with the end tb ... | 2017 | 28734517 |
| identification of mycobacterium tuberculosis enoyl-acyl carrier protein reductase inhibitors: a combined in-silico and in-vitro analysis. | mycobacterium tuberculosis (mtb), had developed evolutionary changes in its genome to adapt for survival and thereby generated multi-drug resistant strains. however, novel drug targets that remained unchanged for their biochemical role has impressed the research community to target such proteins. the comprehensive analysis of multiple protein targets has influenced us to make a consensus structural rule exploited by pharmacophore and other allied techniques from a large repository of protein str ... | 2017 | 28734205 |
| anti-tubercular activity of pyrazinamide is independent of trans-translation and rpsa. | pyrazinamide (pza) is a first line anti-tubercular drug for which the mechanism of action remains unresolved. recently, it was proposed that the active form of pza, pyrazinoic acid (poa), disrupts the ribosome rescue process of trans-translation in mycobacterium tuberculosis. this model suggested that poa binds within the carboxy-terminal domain of ribosomal protein s1 (rpsa) and inhibits trans-translation leading to accumulation of stalled ribosomes. here, we demonstrate that m. tuberculosis rp ... | 2017 | 28733601 |
| a computational protocol for the discovery of lead molecules targeting dna unique to pathogens. | with the rapid emergence of drug resistant pathogens, it has become imperative to develop alternative medications as well as find new drug targets to overcome this crisis. hence, this has become prime focus of several academic laboratories and pharmaceutical companies. here, we report a computational protocol for identifying unique dna sequence(s) in the pathogen which is absent in human and related non-pathogenic strains of the microbe. in order to use the unique sequence as drug target, the pr ... | 2017 | 28733089 |
| multi-analyte validation in heterogeneous solution by elisa. | enzyme linked immunosorbent assay (elisa) is a standard assay that has been used widely to validate the presence of analyte in the solution. with the advancement of elisa, different strategies have shown and became a suitable immunoassay for a wide range of analytes. herein, we attempted to provide additional evidence with elisa, to show its suitability for multi-analyte detection. to demonstrate, three clinically relevant targets have been chosen, which include 16kda protein from mycobacterium ... | 2017 | 28732727 |
| impact of diabetes mellitus on indeterminate results of the quantiferon tb gold in-tube test: a propensity score matching analysis. | the sensitivity of interferon-gamma release assays (igras) in the detection of mycobacterium tuberculosis infection could be affected by conditions of immune dysregulation. for this reason, diabetes mellitus (dm) may increase the frequency of indeterminate results of igras. however, there have been inconsistent reports of role of dm on indeterminate igra results. | 2017 | 28732078 |
| tuberculosis and its particularities in romania and worldwide. | tuberculosis (tb) is a global health issue, with a rising incidence since the beginning of this century. it poses a severe mortality risk and also poses a serious economic risk as it reduces the working capacity of an individual in the most productive part of life. developing countries face widespread tuberculosis infection - up to 95% of all cases and 98% of deaths, respectively. it is a highly contagious infectious disease caused by mycobacterium tuberculosis (the koch bacillus) that can be co ... | 2017 | 28730222 |
| new mycobacterium tuberculosis beijing clonal complexes in china revealed by phylogenetic and bayesian population structure analyses of 24-loci miru-vntrs. | beijing lineage of mycobacterium tuberculosis constitutes the most predominant lineage in east asia. beijing epidemiology, evolutionary history, genetics are studied in details for years revealing probable origin from china followed by worldwide expansion, partially linked to higher mutation rate, hypervirulence, drug-resistance, and association with cases of mixed infections. considering huge amount of data available for 24-loci mycobacterial interspersed repetitive units-variable number of tan ... | 2017 | 28729708 |
| high proportion of modern genotypes of m. tuberculosis and their affinity with drug resistance in northern region of india. | comparative genomics on the basis of tbd1 deletion has differentiated the members of mycobacterium tuberculosis complex (mtc) in two major genogroups. they exhibit differential distribution and virulence potential. the present study was carried out to see the proportion of these genogroups and their association with drug resistance. | 2017 | 28729202 |
| [changes and differences of dna methylation in human macrophages infected with virulent and avirulent mycobacterium tuberculosis]. | objective: to profile dna methylation and compare differentially methylated region (dmr) of macrophages infected with virulent and avirulent strains of mycobacterium tuberculosis (mtb). methods: pma(50 ng/ml) treated thp-1 macrophages were left uninfected or infected with the virulent h37rv(thp-1/rv) or avirulent h37ra(thp-1/ra). the genomic dna was then extracted and dna methylation was profiled via reduced representation bisulfite sequencing (rrbs). the dna methylation pattern and dmrs between ... | 2017 | 28728275 |
| performance of clinical prediction rules for diagnosis of pleural tuberculosis in a high-incidence setting. | diagnosis of pleural tuberculosis (pt) is still a challenge, particularly in resource-constrained settings. alternative diagnostic tools are needed. we aimed at evaluating the utility of clinical prediction rules (cprs) for diagnosis of pleural tuberculosis in peru. | 2017 | 28727272 |
| cyclic di-gmp regulates mycobacterium tuberculosis resistance to ethionamide. | tuberculosis is still on the top of infectious diseases list on both mobility and mortality, especially due to drug-resistance of mycobacterium tuberculosis (m.tb). ethionamide (eth) is one of effective second line anti-tb drugs, a synthetic compound similar to isoniazid (inh) structurally, with existing severe problem of eth resistance. eth is a prodrug, which is activated by etha inside m.tb, and etha is transcriptionally repressed by ethr. we found that c-di-gmp could bind ethr, enhanced the ... | 2017 | 28725053 |
| novel transcriptional signatures for sputum-independent diagnostics of tuberculosis in children. | pediatric tuberculosis (tb) is challenging to diagnose, confirmed by growth of mycobacterium tuberculosis at best in 40% of cases. the who has assigned high priority to the development of non-sputum diagnostic tools. we therefore sought to identify transcriptional signatures in whole blood of indian children, capable of discriminating intra-thoracic tb disease from other symptomatic illnesses. we investigated the expression of 198 genes in a training set, comprising 47 tb cases (19 definite/28 p ... | 2017 | 28724962 |
| co-expression network analysis of toxin-antitoxin loci in mycobacterium tuberculosis reveals key modulators of cellular stress. | research on toxin-antitoxin loci (ta loci) is gaining impetus due to their ubiquitous presence in bacterial genomes and their observed roles in stress survival, persistence and drug tolerance. the present study investigates the expression profile of all the seventy-nine ta loci found in mycobacterium tuberculosis. the bacterium was subjected to multiple stress conditions to identify key players of cellular stress response and elucidate a ta-coexpression network. this study provides direct experi ... | 2017 | 28724903 |
| matrixlysis, an improved sample preparation method for recovery of mycobacteria from animal tissue material. | mycobacterium caprae, a member of the mycobacterium tuberculosis complex, is the main causative agent of bovine tuberculosis in alpine regions. bacterial culture is the gold standard in bovine tuberculosis diagnostic but takes up to twelve weeks. this increases the time and costs for stocks affected with bovine tuberculosis. hence this study focused on the implementation of a fast and precise mycobacterial detection method and compared it with currently used methods. matrix lysis is a chemical l ... | 2017 | 28723969 |
| asymptomatic congenital tuberculosis: a case report. | congenital tuberculosis (tb) is described as a rare, but severe disease. in contrast to the cases with severe symptoms reported so far, we describe a child with asymptomatic congenital tb. | 2017 | 28723785 |
| molecular characterization of mycobacterium tuberculosis strains with tb-sprint. | we evaluated tuberculosis-spoligo-rifampicin-isoniazid typing (tb-sprint), a microbead-based method for spoligotyping and detection of rifampicin and isoniazid resistance in mycobacterium tuberculosis. for that, 67 m. tuberculosis complex strains were retrospectively selected. membrane-based spoligotyping, restriction fragment length polymorphism, dna sequencing/pyrosequencing of rpob, katg, and inha promoter, tb-sprint, and snp typing were performed. concordance between spoligotyping methods wa ... | 2017 | 28722603 |
| mycobacterium tuberculosis infection in close childhood contacts of adults with pulmonary tuberculosis is increased by secondhand exposure to tobacco. | tobacco use is a major risk factor for tuberculosis (tb). secondhand smoke (shs) is also a risk factor for tb and to a lesser extent, mycobacterium tuberculosis infection without disease. we investigated the added risk of m. tuberculosis infection due to shs exposure in childhood contacts of tb cases in the gambia. participants were childhood household contacts aged ≤ 14 years of newly diagnosed pulmonary tb (ptb) cases. the intensity of exposure to the case was categorized according to whether ... | 2017 | 28722570 |
| contribution of brain ct in the diagnosis of tuberculous meningitis: a case report from djibouti. | tuberculous meningitis, a serious disease with high mortality and morbidity, remains frequent in countries with endemic tuberculosis. its non-specific presentation often delays the introduction of appropriate treatment. its definitive diagnosis requires isolation of mycobacterium tuberculosis from cerebrospinal fluid, although this test may be negative without conclusively ruling out this diagnosis. a presumptive diagnosis should be reached as soon as possible through a body of clinical evidence ... | 2017 | 28721934 |
| mapping and characterization of g-quadruplexes in mycobacterium tuberculosis gene promoter regions. | mycobacterium tuberculosis is the causative agent of tuberculosis (tb), one of the top 10 causes of death worldwide in 2015. the recent emergence of strains resistant to all current drugs urges the development of compounds with new mechanisms of action. g-quadruplexes are nucleic acids secondary structures that may form in g-rich regions to epigenetically regulate cellular functions. here we implemented a computational tool to scan the presence of putative g-quadruplex forming sequences in the g ... | 2017 | 28720801 |
| predicting susceptibility to tuberculosis based on gene expression profiling in dendritic cells. | tuberculosis (tb) is a deadly infectious disease, which kills millions of people every year. the causative pathogen, mycobacterium tuberculosis (mtb), is estimated to have infected up to a third of the world's population; however, only approximately 10% of infected healthy individuals progress to active tb. despite evidence for heritability, it is not currently possible to predict who may develop tb. to explore approaches to classify susceptibility to tb, we infected with mtb dendritic cells (dc ... | 2017 | 28720766 |
| serologic responses in childhood pulmonary tuberculosis. | identification of the mycobacterium tuberculosis immunoproteome and antigens associated with serologic responses in adults has renewed interest in developing a serologic test for childhood tuberculosis (tb). we investigated igg antibody responses against m. tuberculosis antigens in children with well-characterized tb. | 2017 | 28719497 |
| case report: disseminated talaromyces (penicillium) marneffei and mycobacterium tuberculosis coinfection in a japanese patient with acquired immunodeficiency syndrome. | talaromyces marneffei is a dimorphic fungus endemic mainly in southeast and south asia. it causes severe mycosis, usually in immunocompromised individuals, such as those with human immunodeficiency virus (hiv) infection. concomitant infection with t. marneffei and other opportunistic pathogens is plausible because the majority of t. marneffei infections occur in patients with advanced hiv infection. nonetheless, coinfection in the same site has rarely been reported, and poses a considerable diag ... | 2017 | 28719322 |
| regional differences in the prevalence of major opportunistic infections among antiretroviral-naïve human immunodeficiency virus patients in japan, northern thailand, northern vietnam, and the philippines. | to identify regional differences in the distribution of opportunistic infections (ois) among human immunodeficiency virus (hiv)-infected patients in asia, the medical records of antiretroviral therapy (art)-naïve patients who attended the following tertiary hospitals from 2003 to 2011 were reviewed: nagoya medical center (nmc, nagoya, japan), lampang hospital (lph, lampang, northern thailand), bach mai hospital (bmh, hanoi, northern vietnam), and philippine general hospital (pgh, manila, philipp ... | 2017 | 28719295 |
| the human microbiome in the fight against tuberculosis. | abstractthe human microbiome is an intriguing potentially modifiable risk factor in our arsenal against mycobacterium tuberculosis, the leading infectious disease killer globally. previous studies have shown associations between the human microbiome and pulmonary disease states; however, etiological links between the microbiome and tuberculosis (tb) infection or disease remain unclear. immunomodulatory roles of the microbiome may prove to be a critical asset in the host response against tb, incl ... | 2017 | 28719264 |
| deletion of sigb causes increased sensitivity to para-aminosalicylic acid and sulfamethoxazole in mycobacterium tuberculosis. | although the de novo folate biosynthesis pathway has been well studied in bacteria, little is known about its regulation. in this study, the sigb gene in mycobacterium tuberculosis (m. tb) was deleted. subsequent drug susceptibility tests revealed that m. tb δsigb was more sensitive to para-aminosalicylic acid (pas) and sulfamethoxazole (smx). comparative transcriptional analysis was performed and down-regulation of pabb was observed in the sigb deleted strain, which was further verified by qrt- ... | 2017 | 28717039 |
| incidence of active tuberculosis in individuals with latent tuberculosis infection in rural china: follow-up results of a population-based, multicentre, prospective cohort study. | the management of latent mycobacterium tuberculosis infection is a new priority action for the who end tuberculosis (tb) strategy. however, national guidelines on latent tuberculosis infection testing and treatment have not yet been developed in china. here, we present the results from the 2-year follow-up of a study that aimed to track the development of active disease in individuals with latent tuberculosis infection, identify priority populations for latent infection management, and explore t ... | 2017 | 28716677 |
| a comparative study of phenotypic and genotypic first- and second-line drug resistance testing of mycobacterium tuberculosis. | this study aimed to evaluate the frequency of resistance to first- and second-line drugs using phenotypic and genotypic methods and its correlation with resistance-linked mutations in mycobacterium tuberculosis (m. tb) isolated in iran. three different methods, including the indirect proportion method(pm), direct and indirect nitrate reductase assay(nra), and direct sequencing were used to assess drug resistance. in this study, sensitivity, specificity, agreement, costs, and turnaround time of t ... | 2017 | 28716625 |
| rapid identification of a mycobacterium tuberculosis full genetic drug resistance profile through whole genome sequencing directly from sputum. | resistance to second-line tuberculosis drugs is common, but slow to diagnose with phenotypic drug sensitivity testing. rapid molecular tests speed up diagnosis, but can only detect limited mutations. whole genome sequencing (wgs) of culture isolates can generate a complete genetic drug resistance profile, but is delayed by the initial culture step. in the case presented here, successful wgs directly from sputum was achieved using targeted enrichment. | 2017 | 28716462 |
| the role of efflux pumps in tuberculosis treatment and their promise as a target in drug development: unraveling the black box. | insight into drug transport mechanisms is highly relevant to the efficacious treatment of tuberculosis (tb). major problems in tb treatment are related to the transport of antituberculosis (anti-tb) drugs across human and mycobacterial membranes, affecting the concentrations of these drugs systemically and locally. firstly, transporters located in the intestines, liver, and kidneys all determine the pharmacokinetics and pharmacodynamics of anti-tb drugs, with a high risk of drug-drug interaction ... | 2017 | 28715978 |
| new findings of toll-like receptors involved in mycobacterium tuberculosis infection. | tuberculosis (tb), an important issue in the present age, affects millions of people each year. the infectious agent of tb, mycobacterium tuberculosis (mtb), interacts with the immune system which prevents the development of this bacterium as much as possible. in fact, the receptors on the surface of immune cells identify the bacteria, one of which is toll-like receptors (tlrs). different tlrs including 2, 4, 9 and 8 play critical roles in tuberculosis infection. in this paper, we focused on the ... | 2017 | 28715935 |
| design, synthesis and 2d qsar study of novel pyridine and quinolone hydrazone derivatives as potential antimicrobial and antitubercular agents. | the increased development of highly resistant bacterial strains and tuberculosis, constitute a serious public health threat, highlighting the urgent need of novel antibacterial agents. in this work, two novel series of nicotinic acid hydrazone derivatives (6a-r) and quinolone hydrazide derivatives (12a-l) were synthesized and evaluated as antimicrobial and antitubercular agents. the synthesized compounds were evaluated in vitro for their antibacterial, antifungal and antimycobacterial activities ... | 2017 | 28715707 |
| antimicrobial activity of organometallic isonicotinyl and pyrazinyl ferrocenyl-derived complexes. | isonicotinyl and pyrazinyl ferrocenyl-derived complexes were prepared using various hydrazides and ferrocenyl aldehydes. three heterobimetallic complexes were also synthesized from the schiff base-derived isonicotinyl ferrocene complex using various platinum group metal dimers based on ruthenium, rhodium and iridium. all complexes were evaluated in vitro for antimycobacterial and antiparasitic activity. against mycobacterium tuberculosis h37rv, the platinum group metal complexes showed glycerol- ... | 2017 | 28713884 |
| role of interferons in the development of diagnostics, vaccines, and therapy for tuberculosis. | tuberculosis (tb) is an airborne infection caused by mycobacterium tuberculosis (mtb). about one-third of the world's population is latently infected with tb and 5-15% of them will develop active tb in their lifetime. it is estimated that each case of active tb may cause 10-20 new infections. host immune response to mtb is influenced by interferon- (ifn-) signaling pathways, particularly by type i and type ii interferons (ifns). the latter that consists of ifn-γ has been associated with the prom ... | 2017 | 28713838 |
| generation of monoclonal antibodies against ag85a antigen of mycobacterium tuberculosis and application in a competitive elisa for serodiagnosis of bovine tuberculosis. | the ag85 complex functions as the main secretory protein of mycobacterium tuberculosis (m. tuberculosis) and bcg. this complex is composed of the proteins, ag85a, ag85b, and ag85c, with ag85a thought to play the largest role within the complex. however, the lack of commercially available monoclonal antibodies (mabs) against ag85a still hinders the biological and applicative research on this protein. in this study, we developed and identified anti-ag85a mabs, and five hybridoma cells were establi ... | 2017 | 28713817 |
| first insight into a nationwide genotypic diversity of mycobacterium tuberculosis among previously treated pulmonary tuberculosis cases in benin, west africa. | molecular studies on tuberculosis (tb) are rare in low-resource countries like benin, where data on molecular study on previously treated tb cases is unavailable. | 2017 | 28713434 |
| nanoparticle-formulated curcumin prevents posttherapeutic disease reactivation and reinfection with mycobacterium tuberculosis following isoniazid therapy. | curcumin, the bioactive component of turmeric also known as "indian yellow gold," exhibits therapeutic efficacy against several chronic inflammatory and infectious diseases. even though considered as a wonder drug pertaining to a myriad of reported benefits, the translational potential of curcumin is limited by its low systemic bioavailability due to its poor intestinal absorption, rapid metabolism, and rapid systemic elimination. therefore, the translational potential of this compound is specif ... | 2017 | 28713372 |
| structural insight into the activation of pkni kinase from m. tuberculosis via dimerization of the extracellular sensor domain. | protein kinases play central roles in the survival of mycobacterium tuberculosis within host. here we report the individual high-resolution crystal structures of the sensor domain (in both monomer and dimer forms) and the kinase domain of pkni, a transmembrane protein member of the serine/threonine protein kinases (stpks) family. pkni is the first stpk identified whose sensor domain exists in a monomer-dimer equilibrium. inspection of the two structures of the sensor domain (pkni_sd) revealed co ... | 2017 | 28712808 |
| synthesis and biological evaluation of novel 1,2,3-triazole derivatives as anti-tubercular agents. | a library of seventeen novel 1,2,3-triazole derivatives were efficiently synthesized in excellent yields by the popular 'click chemistry' approach and evaluated in vitro for their anti-tubercular activity against mycobacterium tuberculosis h37ra (atcc 25177 strain). among the series, six compounds exhibited significant activity with minimum inhibitory concentration (mic) values ranging from 3.12 to 0.78μg/ml and along with no significant cytotoxicity against mbmdmqs (mouse bone marrow derived ma ... | 2017 | 28712709 |
| striking the right immunological balance prevents progression of tuberculosis. | tuberculosis (tb) caused by infection with mycobacterium tuberculosis (mtb) is a major burden for human health worldwide. current standard treatments for tb require prolonged administration of antimycobacterial drugs leading to exaggerated inflammation and tissue damage. this can result in the reactivation of latent tb culminating in tb progression. thus, there is an unmet need to develop therapies that would shorten the duration of anti-tb treatment and to induce optimal protective immune respo ... | 2017 | 28711989 |
| a multistage subunit vaccine effectively protects mice against primary progressive tuberculosis, latency and reactivation. | adult tuberculosis (tb) is the main cause of tb epidemic and death. the infection results mainly by endogenous reactivation of latent tb infection and secondarily transmitted by exogenous infection. there is no vaccine for adult tb. to this end, we first chose antigens from a potential antigenic reservoir. the antigens strongly recognized t cells from latent and active tb infections that responded to antigens expressed by mycobacterium tuberculosis cultured under different metabolic states. fusi ... | 2017 | 28711483 |
| breaking transmission with vaccines: the case of tuberculosis. | members of the mycobacterium tuberculosis complex (mtbc) have evolved causing tuberculosis (tb) in different mammalian hosts. mtbc ecotypes have adapted to diverse animal species, with m. bovis being the most common cause of tb in livestock. cattle-to-human transmission of m. bovis through ingestion of raw milk was common before introduction of the pasteurization process. tb in humans is mainly caused by m. tuberculosis. this bacterium is considered a genetically clonal pathogen that has coevolv ... | 2017 | 28710848 |
| combining molecular typing and spatial pattern analysis to identify areas of high tuberculosis transmission in a moderate-incidence county in taiwan. | in total, 303 randomly selected clinical mycobacterium tuberculosis (mtb) isolates from 303 patients (collected january to december 2012) in central taiwan were examined. the major lineages found were beijing (n = 114, 37.62%), haarlem (n = 76, 25.08%) and east african-indian (eai) (n = 42, 13.86%). notably, younger persons (≤30 years old) were 6.58 times more likely to be infected with a beijing genotype compared to older persons (>70 years) (p < 0.05). combining molecular typing methods and ge ... | 2017 | 28710410 |
| peritoneal tuberculosis as a cause of ascites in a patient with cirrhosis. | a 59-year-old portuguese caucasian man with a history of heavy alcohol intake and no significant medical history presented with ascites, weight loss and general malaise. the ascitic fluid analysis showed 921 cells/mm(3) with mononuclear predominance (93.6%), elevated total proteins and a slightly elevated serum-ascites albumin gradient. the abdominal ultrasound confirmed the presence of chronic liver disease with ascites, and additionally on ct there was evidence of peritoneal thickening. on rep ... | 2017 | 28710241 |
| bactericidal impact of ag, zno and mixed agzno colloidal nanoparticles on h37rv mycobacterium tuberculosis phagocytized by thp-1 cell lines. | the purpose of this research project was to infection of human macrophages (thp-1) cell lines by h37rv strain of mycobacterium tuberculosis (h37rvmtb) and find out the ratio/dilution of mixture silver (ag nps) and zinc oxide nanoparticles (zno nps) whose ability to eliminate phagocytized bacteria compared to rifampicin. the colloidal ag nps and zno nps were synthesized and their characteristics were evaluated. the thp-1 cell lines were infected with different concentration of h37rvmtb. next, the ... | 2017 | 28710015 |
| drug-resistant tuberculosis coexisting with invasive candidiasis in an immunocompetent 30-year-old woman: a case report. | invasive candidiasis coexisting with drug-resistant mycobacterium tuberculosis (dr-tb) in the immunocompetent patient is a rare entity. we report a 30-year-old woman, nondiabetic, who presented to us with complaints of acute onset cough, breathlessness, and fever since 20 days. on thorough investigations, she was diagnosed to be suffering from coexisting drug-resistant tuberculosis and invasive candidiasis. prompt treatment initiated at right time helped us in saving her life. the unique present ... | 2017 | 28709496 |
| band pattern analysis of mutations in rifampicin resistance strain of mycobacterium tuberculosis by line probe assay in patients from delhi, india. | the genotype mtbdrplus, a commercial line probe assay (lpa) kit from hain lifescience, germany, is endorsed by india's rntcp program for diagnosis of drtb cases among smear-positive sputum samples. although the lpa has been studied in several laboratories, there is a wide variation in existing m. tuberculosis strains across the globe, and false results can occur due to the presence of unique genetic mutations in different settings. | 2017 | 28709491 |
| endobronchial ultrasound experience in a high tuberculosis prevalence setting. | most of the published endobronchial ultrasound-guided transbronchial needle aspiration (ebus-tbna) data are from the western countries, establishing the role of ebus-tbna in the diagnosis and staging of lung cancer. the etiology of mediastinal lymphadenopathy may be different in an ethnic group with a high prevalence of tuberculosis (tb). | 2017 | 28709488 |
| diagnosis of tuberculous meningitis: current scenario from a tertiary neurocare centre in india. | tuberculous meningitis (tbm) is a condition that is caused by mycobacterium tuberculosis complex and is difficult to diagnose due to the nonspecificity of the presentations. the study analyzed the different modes of diagnosis available in a developing country set up over a period of five years to understand the diagnostic values of the current conventional and automated methods of diagnosis of tbm among the patients suspected with chronic meningitis. | 2017 | 28709486 |
| parotid tuberculosis. | parotid gland tuberculosis is an uncommon manifestation of one of the most common infections even in the developing countries, caused by mycobacterium tuberculosis. there are no specific symptoms or clinical signs of parotid tuberculosis, and such an infection most commonly presents as a slow growing painless parotid mass. because of its rarity, tuberculosis of parotid gland is often mistaken for a malignant growth, and it most commonly gets diagnosed after superficial protidectomy. complete cur ... | 2017 | 28709482 |
| rv3723/luca coordinates fatty acid and cholesterol uptake in mycobacterium tuberculosis. | pathogenic bacteria have evolved highly specialized systems to extract essential nutrients from their hosts. mycobacterium tuberculosis (mtb) scavenges lipids (cholesterol and fatty acids) to maintain infections in mammals but mechanisms and proteins responsible for the import of fatty acids in mtb were previously unknown. here, we identify and determine that the previously uncharacterized protein rv3723/luca, functions to integrate cholesterol and fatty acid uptake in mtb. rv3723/luca interacts ... | 2017 | 28708968 |
| correction: mycobacterium tuberculosis arrests host cycle at the g1/s transition to establish long term infection. | [this corrects the article doi: 10.1371/journal.ppat.1006389.]. | 2017 | 28708888 |
| rapid diagnosis of extrapulmonary tuberculosis with xpert mycobacterium tuberculosis/rifampicin assay. | xpertmycobacterium tuberculosis/rifampicin (xpert mtb/rif) assay has been endorsed by the world health organization (who) for diagnosis of extrapulmonary tuberculosis (eptb), while the sensitivity and specificity have not been fully evaluated. we aimed to evaluate the performance of xpert mtb/rif assay in the detection of different extrapulmonary specimens. | 2017 | 28708053 |
| a review of recent advancements in anti-tubercular molecular hybrids. | tuberculosis (tb) accounts for millions of deaths worldwide every year. diverse survival strategies adopted by mycobacterium tuberculosis (mtb) has substantially hindered the existing anti-tb regimen thereby leading to multidrug-resistant (mdr) and extremely drug-resistant (xdr) strains of tb. the steady decrease in current antibiotics' efficacy against these adversities is an indicator that their adequate replenishment in future is almost impossible, placing society on the precipice of a catast ... | 2017 | 28707584 |
| misdiagnosis of tuberculosis associated with some species of nontuberculous mycobacteria by genexpert mtb/rif assay. | out of 12 nontuberculous mycobacteria (ntm) species, 5 were identified as mycobacterium tuberculosis (mtb) by genexpert at a bacterial load of 10(6). notably, two species, including m. abscessus and m. smegmatis, were flagged as rif-resistant mtb due to the high c(t) value of probe e. in conclusion, our data have demonstrated that the misdiagnosis of mtb by genexpert assay is observed in five ntm species at a high bacterial load. | 2017 | 28707184 |
| host sirtuin 1 regulates mycobacterial immunopathogenesis and represents a therapeutic target against tuberculosis. | mycobacterium tuberculosis (mtb) executes a plethora of immune-evasive mechanisms, which contribute to its pathogenesis, limited efficacy of current therapy, and the emergence of drug-resistant strains. this has led to resurgence in attempts to develop new therapeutic strategies/targets against tuberculosis (tb). we show that mtb down-regulates sirtuin 1 (sirt1), a nicotinamide adenine dinucleotide (nad(+))-dependent deacetylase, in monocytes/macrophages, tb animal models, and tb patients with a ... | 2017 | 28707004 |
| reactogenicity to major tuberculosis antigens absent in bcg is linked to improved protection against mycobacterium tuberculosis. | mtbvac is a live-attenuated mycobacterium tuberculosis vaccine, currently under clinical development, that contains the major antigens esat6 and cfp10. these antigens are absent from the current tuberculosis vaccine, bcg. here we compare the protection induced by bcg and mtbvac in several mouse strains that naturally express different mhc haplotypes differentially recognizing esat6 and cfp10. mtbvac induces improved protection in c3h mice, the only of the three tested strains reactive to both es ... | 2017 | 28706226 |
| reduced susceptibility of clinical strains of mycobacterium tuberculosis to reactive nitrogen species promotes survival in activated macrophages. | drugs such as isoniazid (inh) and pretomanid (prt), used against mycobacterium tuberculosis are active partly through generation of reactive nitrogen species (rns). the aim of this study was to explore variability in intracellular susceptibility to nitric oxide (no) in clinical strains of m. tuberculosis. | 2017 | 28704501 |
| synthesis and in vitro antimycobacterial and antibacterial activity of 8-ome ciprofloxacin-hydrozone/azole hybrids. | a series of novel 8-ome ciprofloxacin (cpfx)-hydrazone/azole hybrids were designed, synthesized, and evaluated for their in vitro biological activities. our results reveal that all of the hydrozone-containing hybrids (except for 7) show potency against mycobacterium tuberculosis (mtb) h37rv (minimum inhibitory concentration (mic): <0.5 μm), which is better than the parent drug cpfx, and comparable to moxifloxacin and isoniazid, some of the tested gram-positive strains (mic: 0.06-4 μg/ml), and mo ... | 2017 | 28703766 |
| the role of human host genetics in tuberculosis resistance. | tuberculosis (tb) remains a public health problem: the latest estimate of new incident cases per year is a staggering 10.4 million. despite this overwhelming number, the majority of the immunocompetent population can control infection with mycobacterium tuberculosis. the human genome underlies the immune response and contributes to the outcome of tb infection. areas covered: investigations of tb resistance in the general population have closely mirrored those of other infectious diseases and ini ... | 2017 | 28703045 |
| tuberculosis and pneumonia in hiv-infected children: an overview. | pneumonia remains the most common cause of hospitalization and the most important cause of death in young children. in high human immunodeficiency virus (hiv)-burden settings, hiv-infected children carry a high burden of lower respiratory tract infection from common respiratory viruses, bacteria and mycobacterium tuberculosis. in addition, pneumocystis jirovecii and cytomegalovirus are important opportunistic pathogens. as the vertical transmission risk of hiv decreases and access to antiretrovi ... | 2016 | 28702298 |
| novel vaccination approaches to prevent tuberculosis in children. | pediatric tuberculosis (tb) is an underappreciated problem and accounts for 10 % of all tb deaths worldwide. children are highly susceptible to infection with mycobacterium tuberculosis and interrupting tb spread would require the development of effective strategies to control tb transmission in pediatric populations. the current vaccine for tb, m. bovis bacille calmette-guérin (bcg), can afford some level of protection against tb meningitis and severe forms of disseminated tb in children; howev ... | 2016 | 28702297 |
| the conserved actinobacterial two-component system mtrab coordinates chloramphenicol production with sporulation in streptomyces venezuelae nrrl b-65442. | streptomyces bacteria make numerous secondary metabolites, including half of all known antibiotics. production of antibiotics is usually coordinated with the onset of sporulation but the cross regulation of these processes is not fully understood. this is important because most streptomyces antibiotics are produced at low levels or not at all under laboratory conditions and this makes large scale production of these compounds very challenging. here, we characterize the highly conserved actinobac ... | 2017 | 28702006 |
| fermentation, isolation, purification and characterization of an antitubercular antibiotic from streptomyces luridus mtcc 4402. | tuberculosis, caused by mycobacterium tuberculosis, continues to be a serious public health problem around the world, and it urges the need for development of new antitubercular drugs. an antibiotic producing strain, streptomyces luridus (mtcc 4402) was earlier isolated from soil by our group. in this work, the phylogenic status was established by 16s rrna gene sequence analysis. the strain was found to be active against clinically resistant strains. the culture was grown in shake flasks in a me ... | 2016 | 28699723 |
| cell wall associated factors of mycobacterium tuberculosis as major virulence determinants: current perspectives in drugs discovery and design. | mycobacteria genus is responsible for deadly diseases like tuberculosis and leprosy. cell wall of bacteria belonging to this genus is unique in many ways. it plays a major role in pathogenesis and intracellular survival inside the host. in intracellular pathogens, their cell wall acts as molecular shield and interacts with host cell milieu to modulate host defense responses. unique role of cell wall associated factors that play crucial roles in the biosynthesis of mycobacterial cell wall compone ... | 2017 | 28699515 |
| tuberculosis in kidney transplant recipients: a case series. | solid organ transplant recipients have an elevated risk of tuberculosis (tb) with high mortality. data about tb in this population in the united states is sparse. we present four cases of active tuberculosis in kidney transplant recipients at our center. all patients had possible tb exposure prior to transplant and all were diagnosed with active tb within the first year of transplant. disseminated tb was seen in half of the patients with extra-pulmonary tb being more common affecting lymph nodes ... | 2017 | 28698838 |
| antigens rv0310c and rv1255c are promising novel biomarkers for the diagnosis of mycobacterium tuberculosis infection. | this study aimed to identify novel immunogenic epitopes from mycobacterium tuberculosis (mtb) that could be used in tuberculosis (tb) diagnostics. to determine the diagnostic potential of mycobacterial antigens in serodiagnosis of tb, 256 patients were enrolled in a study and divided into two groups: 126 smear-positive pulmonary tb patients (sppt) and 130 smear-negative pulmonary tb patients (snpt); 152 bacillus calmette-guerin (bcg)-vaccinated healthy people were used as a control. murine resul ... | 2017 | 28698665 |
| a novel protein kinase inhibitor imb-yh-8 with anti-tuberculosis activity. | protein kinase b (pknb) is one of the mycobacterium tuberculosis serine/threonine protein kinases and has an essential role in sustaining mycobacterial growth. here, we identified and characterized a novel small molecule compound named imb-yh-8 that inhibited pknb and served as anti-mycobacteria lead compound. imb-yh-8 inhibited pknb auto-phosphorylation and the phosphorylation of gara by pknb in a dose-dependent manner. the compound did not inhibit human akt1 or other serine/threonine kinases i ... | 2017 | 28698545 |
| multidrug-resistant/extensively drug-resistant tuberculosis in greece: predominance of mycobacterium tuberculosis genotypes endemic in the former soviet union countries. | | 2017 | 28698038 |
| pyrazinamide resistance-conferring mutations in pnca and the transmission of multidrug resistant tb in georgia. | the ongoing epidemic of multidrug-resistant tuberculosis (mdr-tb) in georgia highlights the need for more effective control strategies. a new regimen to treat mdr-tb that includes pyrazinamide (pza) is currently being evaluated and pza resistance status will largely influence the success of current and future treatment strategies. pza susceptibility testing was not routinely performed at the national reference laboratory (nrl) in tbilisi between 2010 and september 2015. we here provide a first i ... | 2017 | 28697808 |
| plumbago auriculata leaf extract-mediated agnps and its activities as antioxidant, anti-tb and dye degrading agents. | in the present work, silver nanoparticles have been biosynthesized by utilizing the alcoholic extract of plumbago auriculata. the optimization of reaction conditions was carried out by monitoring the reactions with the help of uv-visible absorption spectroscopy. the characterization of agnp was carried out by infrared spectroscopy, transmission electron microscopy and x-ray diffraction (xrd) studies. the biogenic agnps were tested against mycobacterium tuberculosis using microplate almar blue as ... | 2017 | 28697688 |
| ethambutol partitioning in tuberculous pulmonary lesions explains its clinical efficacy. | clinical trials and practice have shown that ethambutol is an important component of the first-line tuberculosis (tb) regime. this contrasts the drug's rather modest potency and lack of activity against nongrowing persister mycobacteria. the standard plasma-based pharmacokinetic-pharmacodynamic profile of ethambutol suggests that the drug may be of limited clinical value. here, we hypothesized that this apparent contradiction may be explained by favorable penetration of the drug into tb lesions. ... | 2017 | 28696241 |
| sterilizing effect of ertapenem-clavulanate in a hollow-fiber model of tuberculosis and implications on clinical dosing. | carbapenems are now being explored for treatment of multidrug-resistant tuberculosis (mdr-tb), especially in conjunction with clavulanate. clinical use is constrained by the need for multiple parenteral doses per day and the lack of knowledge of the optimal dose for sterilizing effect. our objective was to identify the ertapenem exposure associated with optimal sterilizing effect and then design a once-a-day dose for clinical use. we utilized the hollow-fiber system model of tuberculosis in a 28 ... | 2017 | 28696238 |
| innate scavenger receptor-a regulates adaptive t helper cell responses to pathogen infection. | the pattern recognition receptor (prr) scavenger receptor class a (sr-a) has an important function in the pathogenesis of non-infectious diseases and in innate immune responses to pathogen infections. however, little is known about the role of sr-a in the host adaptive immune responses to pathogen infection. here we show with mouse models of helminth schistosoma japonicum infection and heat-inactivated mycobacterium tuberculosis stimulation that sr-a is regulated by pathogens and suppresses irf5 ... | 2017 | 28695899 |
| the cording phenotype of mycobacterium tuberculosis induces the formation of extracellular traps in human macrophages. | the causative agent of tuberculosis, mycobacterium tuberculosis, shares several characteristics with organisms that produce biofilms during infections. one of these is the ability to form tight bundles also known as cords. however, little is known of the physiological relevance of the cording phenotype. in this study, we investigated whether cord-forming m. tuberculosis induce the formation of macrophage extracellular traps (mets) in human monocyte-derived macrophages. macrophages have previousl ... | 2017 | 28695112 |
| abdominal tuberculosis after removal of an adjustable gastric band - report of an unusual case. | laparoscopic adjustable gastric banding (lagb) is the third most popular bariatric procedure. despite its reversibility and minimal invasiveness, band infection affects 1.2% of patients. we present a case of a 25-year-old, obese woman who was experiencing malaise and feverishness 3 years after gastric band placement. due to port site infection the port was removed, which did not improve the patient's condition. after 2 years the band was removed via laparotomy with a minor surgical site infectio ... | 2017 | 28694906 |
| an uncommon cause of longitudinally extensive transverse myelitis. | considering that currently romania has the highest tuberculosis incidence in europe, the recognition of the infection is an acknowledged health issue. central nervous system tuberculosis accounts for approximately 1% of all cases of tuberculosis. longitudinally extensive transverse myelitis is a contiguous infl ammatory lesion of the spinal cord which involves three or more spinal segments. in tuberculosis, spinal cord involvement is rare and is usually rather due to radiculomyelitis or spinal t ... | 2016 | 28694861 |
| mycobacterium tuberculosis dosr regulon gene rv2004c encodes a novel antigen with pro-inflammatory functions and potential diagnostic application for detection of latent tuberculosis. | approximately 1.7 billion people in the world harbor latent mycobacterium tuberculosis (mtb) with a substantial risk of progression to clinical outcome. containment of these seed beds of mtb is essential to eliminate tuberculosis completely in high burden settings such as india. hence, there is an urgent need for the identification of new serological markers for detection or vaccine candidates to prevent latent tuberculosis infection (ltbi). dosr regulon antigens of mtb might serve as attractive ... | 2017 | 28694808 |
| medicinal plants used in the treatment of tuberculosis - ethnobotanical and ethnopharmacological approaches. | tuberculosis is a highly infectious disease declared a global health emergency by the world health organization, with approximately one third of the world's population being latently infected with mycobacterium tuberculosis. tuberculosis treatment consists in an intensive phase and a continuation phase. unfortunately, the appearance of multi drug-resistant tuberculosis, mainly due to low adherence to prescribed therapies or inefficient healthcare structures, requires at least 20months of treatme ... | 2017 | 28694178 |
| systematic review and meta-analysis of the nitrate reductase assay for drug susceptibility testing of mycobacterium tuberculosis and the detection limits in liquid medium. | recently, the need for rapid, reliable, and low-cost drug susceptibility testing (dst) methods has increased due to the emergence of multidrug-resistant mycobacterium tuberculosis. colorimetric methods of dst provide results more quickly than standard culture methods and are inexpensive than molecular methods. thus, colorimetric methods, such as the nitrate reductase assay (nra), are being recommended. we searched medline pubmed for reports on the nra for dst of m. tuberculosis written in englis ... | 2017 | 28694139 |
| hydrophilic interaction liquid chromatography-mass spectrometry as a new tool for the characterization of intact semi-synthetic glycoproteins. | improved methods for detailed characterization of complex glycoproteins are required in the growing sector of biopharmaceuticals. hydrophilic interaction liquid chromatography (hilic) coupled to high resolution (hr) time-of-flight mass spectrometric (tof-ms) detection was examined for the characterization of intact neo-glycoproteins prepared by chemical conjugation of synthetic saccharides to the lysine residues of selected recombinant proteins. the separation performances of three different ami ... | 2017 | 28693734 |
| evaluation of the relationship between marco and cd36 single-nucleotide polymorphisms and susceptibility to pulmonary tuberculosis in a chinese han population. | gene polymorphisms impact greatly on a person's susceptibility to pulmonary tuberculosis (ptb). macrophage receptor with collagenous structure (marco) and cd36 are two scavenger receptors (srs) that can recognize mycobacterium tuberculosis (mtb) and play a key role in tuberculosis infection. gene polymorphisms of marco and cd36 may contribute to tuberculosis risk. | 2017 | 28693442 |
| whole genome sequencing of mycobacterium tuberculosis isolates from extrapulmonary sites. | tuberculosis (tb) remains one of the leading causes of morbidity and mortality worldwide. extrapulmonary tuberculosis (eptb) constitutes around 15-20% of tb cases in immunocompetent individuals. extrapulmonary sites that are affected by tb include bones, lymph nodes, meningitis, pleura, and genitourinary tract. whole genome sequencing has emerged as a powerful tool to map genetic diversity among mycobacterium tuberculosis (mtb) isolates and identify the genomic signatures associated with drug re ... | 2017 | 28692415 |
| structural and biophysical characterization of the mycobacterium tuberculosis protein rv0577, a protein associated with neutral red staining of virulent tuberculosis strains and homologue of the streptomyces coelicolor protein kbpa. | mycobacterium tuberculosis protein rv0577 is a prominent antigen in tuberculosis patients, the component responsible for neutral red staining of virulent strains of m. tuberculosis, a putative component in a methylglyoxal detoxification pathway, and an agonist of toll-like receptor 2. it also has an amino acid sequence that is 36% identical to that of streptomyces coelicolor afsk-binding protein a (kbpa), a component in the complex secondary metabolite pathways in the streptomyces genus. to gain ... | 2017 | 28692281 |
| the emergence of phenolic glycans as virulence factors in mycobacterium tuberculosis. | tuberculosis is the leading infectious cause of mortality worldwide. the global epidemic, caused by mycobacterium tuberculosis, has prompted renewed interest in the development of novel vaccines for disease prevention and control. the cell envelope of m. tuberculosis is decorated with an assortment of glycan structures, including glycolipids, that are involved in disease pathogenesis. phenolic glycolipids and the structurally related para-hydroxybenzoic acid derivatives display potent immunomodu ... | 2017 | 28692249 |
| case report of a lupus patient with a severe flare and miliary tuberculosis: need for proper guidelines for management. | systemic lupus erythematosus (sle) is an autoimmune disease that is frequently treated with high doses of corticosteroids and other immunosuppressive drugs. thus patients with sle are at increased risk for infections with several pathogens including mycobacterium tuberculosis. there are no established guidelines available for treatment of tuberculosis in sle patients with high disease activity due to lack of relevant studies and management based more on physician expertise. we report a case of a ... | 2017 | 28690859 |
| identification of inhibitors against α-isopropylmalate synthase of mycobacterium tuberculosis using docking-mm/pbsa hybrid approach. | α-isopropylmalate synthase (α-ipms) encoded by leua in mycobacterium tuberculosis (m.tb) is involved in the leucine biosynthesis pathway and is extremely critical for the synthesis of branched-chain amino acids (leucine, isoleucine and valine). α-ipms activity is required not only for the proliferation of m.tb but is also indispensable for its survival during the latent phase of infection. it is absent in humans and is widely regarded as one of the validated drug targets against tuberculosis (tb ... | 2017 | 28690380 |
| [diagnostic value of different detection methods for mycobacterium tuberculosis in bronchoalveolar lavage fluid from pulmonary tuberculosis patients]. | to investigate the diagnostic value of different detection methods for mycobacterium tuberculosis in bronchoalveolar lavage fluid (balf) from patients with pulmonary tuberculosis.
methods: balf from100 patients in changsha central hospital from january 2013 to december 2015 was collected. among 100 patients, 65 cases were clinically diagnosed as tuberculosis, and 35 cases served as control. balf smear method, polymerase chain reaction (pcr) and membrane reverse dot blot (rdb) were used for sync ... | 2017 | 28690221 |
| combination of commercially available molecular assays and culture based methods in diagnosis of tuberculosis and drug resistant tuberculosis. | early diagnosis of tuberculosis is of major clinical importance. among 4733 clinical specimens collected from 3363 patients and subjected to ziehl-neelsen microscopy, 4109 were inoculated onto löwenstein-jensen slants and 3139 in bactec/9000mb. polymerase chain reaction (pcr) was performed in 3139 specimens, whereas, a genotypic assay was directly applied in 93 mycobacterium tuberculosis complex pcr-positive for isoniazid and rifampicin resistance detection specimens (genotype mtbdrplus). recove ... | 2017 | 28689813 |
| invasive mucinous adenocarcinoma with lepidic-predominant pattern coexisted with tuberculosis: a case report. | we observed a rare case of invasive mucinous adenocarcinoma (ima) with a lepidic-predominant pattern accompanied by pulmonary tuberculosis. an 85-year-old man with repeated cough and sputum was admitted to xinhua hospital. t-spot test result was 212 pg/ml (reference value of negative is < 14 pg/ml), mycobacterium tuberculosis culture was positive, and tuberculin skin test (ppd) was negative (skin induration < 5 mm). the patient was treated with several courses of antibiotics and anti-tuberculosi ... | 2017 | 28687977 |
| a review of eleven cases of tuberculosis presenting as sternal wound abscess after open heart surgery. | sternal wound infection with mycobacterium tuberculosis is an uncommon yet highly challenging disease that can be quite insidious with various presentations. we hereby provide a review of 10 cases in current literature and describe an additional case which illustrates the difficulties associated with diagnosis. | 2017 | 28687049 |
| proteomic profile of mycobacterium tuberculosis after eupomatenoid-5 induction reveals potential drug targets. | we investigated a proteome profile, protein-protein interaction and morphological changes of mycobacterium tuberculosis after different times of eupomatenoid-5 (eup-5) induction to evaluate the cellular response to the drug-induced damages. | 2017 | 28686056 |
| human genetic factors in tuberculosis: an update. | tuberculosis (tb) is a major threat to human health, especially in many developing countries. human genetic variability has been recognised to be of great relevance in host responses to mycobacterium tuberculosis infection and in regulating both the establishment and the progression of the disease. an increasing number of candidate gene and genome-wide association studies (gwas) have focused on human genetic factors contributing to susceptibility or resistance to tb. to update previous reviews o ... | 2017 | 28685916 |