| impact of the gen-probe amplified mtd® test on tuberculosis diagnosis in children. | to evaluate the performance of the gen-probe amplified mtd® test (amtd) for childhood tuberculosis (tb) diagnosis compared to conventional culture. | 2012 | 22230051 |
| designation of subtypes of mycobacterium tuberculosis beijing family: an issue of priority. | | 2012 | 22229272 |
| t cell responses to dosr and rpf proteins in actively and latently infected individuals from colombia. | mycobacterium tuberculosis dosr regulon-encoded proteins elicit strong immune t-cell responses in individuals with latent tuberculosis (ltbi). also, resuscitation (rpf) proteins can induce such responses. however, variations in the immunogenicity of the dosr and rpf proteins have been observed in european and african populations, and no data are published from other geographic areas. in colombian ltbi and patients with recently diagnosed ptb, we therefore studied the immune response to dosr, rpf ... | 2012 | 22226907 |
| comparison of dna extraction protocols for mycobacterium tuberculosis in diagnosis of tuberculous meningitis by real-time polymerase chain reaction. | several nucleic acid amplification techniques are available for detection of mycobacterium tuberculosis (mtb) in pulmonary and extrapulmonary samples, but insufficient data are available on the diagnostic utility of these techniques in tubercular meningitis where bacilli load is less. the success of final amplification and detection of nucleic acid depends on successful extraction of dna from the organism. | 2011 | 22223998 |
| evaluation of toll-like receptor and adaptor molecule polymorphisms for susceptibility to tuberculosis in a colombian population. | immunological studies have supported the idea that innate immunity is critical for the control of mycobacterium tuberculosis (mtb) infection in humans. despite the overwhelming evidence showing the critical role of toll-like receptors (tlrs) in the in vitro recognition of mtb, the in vivo significance of individual tlrs has been more difficult to demonstrate consistently. we were interested in examining the role of genes of tlrs and molecules involved in their signalling cascades, and a case-con ... | 2012 | 22221660 |
| tuberculous orchiepididymitis, meningoencephalitis and hydrocephalus. | tuberculous meningoencephalitis (tbm) is a rare and serious, often fatal presentation of active tuberculosis and account for about 1% of cases. early diagnosis and prompt treatment of tbm is essential to reduce morbidity and mortality. here, we report a case of tbm in 60-year-old man. tbm was considered on the basis of clinical presentation, laboratory findings (hyponatraemia), cerebrospinal fluid studies, radiological findings (hydrocephalus on multi-slice computed tomography), and history of o ... | 2011 | 22220454 |
| the mycobacterium tuberculosis fas-ii dehydratases and methyltransferases define the specificity of the mycolic acid elongation complexes. | the human pathogen mycobacterium tuberculosis (mtb) has the originality of possessing a multifunctional mega-enzyme fas-i (fatty acid synthase-i), together with a multi-protein fas-ii system, to carry out the biosynthesis of common and of specific long chain fatty acids: the mycolic acids (ma). ma are the main constituents of the external mycomembrane that represents a tight permeability barrier involved in the pathogenicity of mtb. the ma biosynthesis pathway is essential and contains targets f ... | 2011 | 22216317 |
| granulysin-expressing cd4+ t cells as candidate immune marker for tuberculosis during childhood and adolescence. | granulysin produced by cytolytic t cells directly contributes to immune defense against tuberculosis (tb). we investigated granulysin as a candidate immune marker for childhood and adolescent tb. | 2011 | 22216262 |
| [tuberculous peritonitis during etanercept therapy for rheumatoid arthritis]. | in august 2010, a 73-year-old woman with rheumatoid arthritis receiving etanercept (etn) therapy for two years, developed high-fever and abdominal fullness. though she had not been exposed to tuberculosis, isoniazid prophylaxis was administrated. antibiotics were not effective. ct images revealed the massive ascites and peritonitis, and ga scintigraphy demonstrated notable uptake in the peritoneum. ascites analysis showed an elevated adenosine deaminase activity value (104.9 iu/l) without malign ... | 2011 | 22214811 |
| [analysis of molecular mechanisms of the virulence and growth coordination of mycobacterium tuberculosis]. | | 2011 | 22214749 |
| dihalogenated sulfanilamides and benzolamides are effective inhibitors of the three β-class carbonic anhydrases from mycobacterium tuberculosis. | a series of halogenated sulfanilamides and halogenated benzolamide derivatives have been investigated as inhibitors of three β-carbonic anhydrases (cas, ec 4.2.1.1) from the bacterial pathogen mycobacterium tuberculosis, mtca 1 (rv1284), mtca 2 (rv3588c) and mtca 3 (rv3273). all three enzymes were inhibited with efficacies between the submicromolar to the micromolar one, depending on the substitution pattern at the sulfanilamide moiety/fragment of the molecule. best inhibitors were the halogenat ... | 2013 | 22214209 |
| rapid molecular detection of tuberculosis and rifampicin drug resistance: retrospective analysis of a national u.k. molecular service over the last decade. | fast and reliable detection of mycobacterium tuberculosis complex (mtbc) and drug resistance is crucial in establishing effective treatment and enforcing timely public health measures. | 2012 | 22213739 |
| gelation behavior of 5-chloro-8-hydroxyquinoline, an antituberculosis agent in aqueous alcohol solutions. | it was shown that 5-chloro-8-hydroxyquinoline, an antituberculosis agent, gels aqueous alcohol solutions efficiently. thermal stability and gel-to-sol transition temperature of 1% gel in cd₃od/d₂o (2:1) was studied by ¹h-nmr. fibrous structures of four xerogels have been characterized by scanning electron microscope. | 2012 | 27029417 |
| construction of a genome-scale kinetic model of mycobacterium tuberculosis using generic rate equations. | the study of biological systems at the genome scale helps us understand fundamental biological processes that govern the activity of living organisms and regulate their interactions with the environment. genome-scale metabolic models are usually analysed using constraint-based methods, since detailed rate equations and kinetic parameters are often missing. however, constraint-based analysis is limited in capturing the dynamics of cellular processes. in this paper, we present an approach to build ... | 2012 | 24957639 |
| [the macrophage: chief of tuberculosis immune response]. | with nearly eight million new cases each year and two million deaths, tuberculosis (tb) remains a major health problem worldwide. the limited protection afforded by the only available vaccine, bacille calmette-guerin bcg, and the emergence of multi-resistant strains to antibiotics along with the advent of aids, are three main causes that contributed to the increase of tb incidence during the last decade. the world health organization (who) estimates between 2000 and 2020, nearly one billion peop ... | 2012 | 24834657 |
| distinguishing latent from active mycobacterium tuberculosis infection using elispot assays: looking beyond interferon-gamma. | mycobacterium tuberculosis (mtb) is a global heath epidemic, its threat amplified by hiv infection and the emergence of multidrug-resistant tuberculosis (mdr-tb). interferon (ifn)-gamma release assays (igras) have improved the accuracy of detection of mtb exposure in some subject groups as compared to the tuberculin skin test (tst). however, as ifn-gamma is produced by both fully rested and more recently activated populations of memory t cells, it is not surprising that the measurement of this c ... | 2012 | 24710416 |
| genitourinary tuberculosis: historical and basic science review: past and present. | genitourinary tuberculosis (gutb) usually results from the reactivation of old, dormant tuberculous diseases by pathogens of the mycobacterium tuberculosis complex. gutb is the second most common form of extrapulmonary tuberculosis, with more than 90% of cases occurring in developing countries. in gutb, the kidneys are the most common sites of infection and are infected through hematogenous spread of the bacilli, which then spread through the renal and urinary tract. patients with genital and ur ... | 2012 | 24578959 |
| in vitro evaluation of tetrazoles as a novel class of antimycobacterium tuberculosis agents. | we report here the antimycobacterial activity of some already synthesized tetrazole derivatives containing tetrazole against mycobacterium tuberculosis strain h37rv. | 2012 | 24312768 |
| levofloxacin-proliposomes: opportunities for use in lung tuberculosis. | levofloxacin (lev) is a relatively new-generation fluoroquinolone antibiotic that has good activity against mycobacterium tuberculosis. the aims of this study were to develop and evaluate lev-proliposomes in a dry powder aerosol form for pulmonary delivery. lev-proliposomes containing lev, soybean phosphatidylcholine, cholesterol and porous mannitol were prepared by a spray drying technique. the physicochemical properties of lev-proliposomes were determined using a cascade impactor, x-ray diffra ... | 2012 | 24300299 |
| tuberculosis in the intensive care unit: a retrospective descriptive cohort study with determination of a predictive fatality score. | despite effective treatments, tuberculosis-related mortality remains high among patients requiring admission to the intensive care unit (icu). | 2012 | 24294270 |
| the role of transport mechanisms in mycobacterium tuberculosis drug resistance and tolerance. | in the fight against tuberculosis, cell wall permeation of chemotherapeutic agents remains a critical but largely unsolved question. here we review the major mechanisms of small molecule penetration into and efflux from mycobacterium tuberculosis and other mycobacteria, and outline how these mechanisms may contribute to the development of phenotypic drug tolerance and induction of drug resistance. m. tuberculosis is intrinsically recalcitrant to small molecule permeation thanks to its thick lipi ... | 2012 | 24281307 |
| why and how the old neuroleptic thioridazine cures the xdr-tb patient. | this mini-review provides the entire experimental history of the development of the old neuroleptic thioridazine (tz) for therapy of antibiotic resistant pulmonary tuberculosis infections. tz is effective when used in combination with antibiotics to which the initial mycobacterium tuberculosis was resistant. under proper cardiac evaluation procedures, the use of tz is safe and does not produce known cardiopathy such as prolongation of qt interval. because tz is cheap, it should be considered for ... | 2012 | 24280703 |
| the chemical components of sesbania grandiflora root and their antituberculosis activity. | three isoflavanoids, isovestitol (1), medicarpin (2), and sativan (3), along with another known compound, betulinic acid (4), were isolated from the root of sesbania grandiflora. the structures of the isolated compounds were characterised by means of spectroscopic techniques (uv, ir, ms, 1h- and 13c-nmr, dept, cosy, hmqc, hmbc, and ms analysis). all the tested compounds 1-4 exhibited antituberculosis activity against mycobacterium tuberculosis h37rv, with mic values of 50 µg/ml for compounds 1-3 ... | 2012 | 24280680 |
| anti-mycobacterium tuberculosis activity of fungus phomopsis stipata. | our purpose was to determine the anti-mycobacterium tuberculosis activity of the metabolites produced by the endophitic fungus phomopsis stipata (lib.) b. sutton, (diaporthaceae), cultivated in different media. the antimycobacterial activity was assessed through the resazurin microtiter assay (rema) and the cytotoxicity test performed on macrophage cell line. the extracts derived from fungi grown on corn medium and potato dextrose broth presented the smallest values of minimum inhibitory concent ... | 2012 | 24031821 |
| pott's disease: a case of mycobacterium xenopi infection of the spine. | pott's disease is an infection of the spine with mycobacterium tuberculosis that causes destruction of the spine elements resulting in progressive kyphosis. we are describing a rare case of pott's disease where mycobacterium xenopi was the inculpated organism. | 2012 | 23882381 |
| [antituberculosis properties of methyldioxotetrahydropyrimidine sulfonisonicotinoyl hydrazide]. | antituberculosis properties of methyldioxotetrahydropyrimidine sulfonisonicotinoyl hydrazide (msh) registered in russia under the name of tubosan of the class of immunotropic agents were investigated with the use of 78 clinical isolates of mycobacterium tuberculosis (mbt). in concentration of 60 or 80 mcg/ml the drug showed significant antituberculosis activity. the effect of msh on drug susceptible mbt was bactericidal. the effect of msh on the drug resistant mbt (with the isoniazid mic of 1 mc ... | 2012 | 23700932 |
| atypical cause of stroke in a 27 year old male. | central nervous system (cns) tuberculosis (tb) meningitis is a progressive disease that can present in many forms. cns tb is encountered frequently in areas of high prevalence of tuberculosis and dissemination is common, but tb meningitis is a disease that has a relatively low prevalence in north america. | 2012 | 23569494 |
| tissue is issue: not all masses are tumors. | tuberculosis can disguise itself in any form. endobronchial tuberculosis usually presents in young adults. endobronchial tumor-like presentation of tuberculosis (ebtb) is very rare and often mistaken as a malignancy. diagnosis is usually delayed, as clinical and radiological features are non-specific. direct implantation of tubercle bacilli into the bronchus, or an contiguous spread, leads to ebtb. bronchoscopic biopsy and culture are the best modality for diagnosis. care reports: clinical and r ... | 2012 | 23569487 |
| [evaluation of tb-beads assay utilizing the technique of magnetic beads--an innovative assay method for detection of acid fast bacilli]. | the centrifuge method with the use of semi-alkalin proteinase (sap) and nalc-naoh, recommended by the "2007 edition of the assay guideline for detection of mycobacterium tuberculosis," has significantly contributed to improving the sensitivities and specificities of both smear and culture tests for detection of acid fast bacilli (afb). however, this method poses some challenges in terms of its cumbersome and time-consuming assay protocol. "tb-beads (kyokuto pharmaceutical industrial co., ltd.)" ... | 2012 | 23547479 |
| high proportion of fluoroquinolone-resistant mycobacterium tuberculosis isolates with novel gyrase polymorphisms and a gyra region associated with fluoroquinolone susceptibility. | fluoroquinolone resistance in mycobacterium tuberculosis can be conferred by mutations in gyra or gyrb. the prevalence of resistance mutations outside the quinolone resistance-determining region (qrdr) of gyra or gyrb is unclear, since such regions are rarely sequenced. m. tuberculosis isolates from 1,111 patients with newly diagnosed culture-confirmed tuberculosis diagnosed in tennessee from 2002 to 2009 were screened for phenotypic ofloxacin resistance (>2 μg/ml). for each resistant isolate, t ... | 2012 | 22189117 |
| antigen-specific multifunctional t-cells in sarcoidosis patients with lofgren's syndrome. | sarcoidosis is a granulomatous disease of unknown aetiology, mainly affecting the lungs. recently, t-cell responses towards a specific mycobacterial protein, catalase-peroxidase (mkatg), were observed in sarcoidosis patients. bronchoalveolar lavage (bal) fluid and peripheral blood were obtained from a total of 23 sarcoidosis patients, of whom 13 had löfgren's syndrome and lung accumulations of t-cell receptor av2s3+ t-cells. using six-colour flow cytometry in combination with intracellular cytok ... | 2012 | 22183480 |
| immune regulatory activities of early secreted antigenic target of 6-kd protein of mycobacterium tuberculosis and implications for tuberculosis vaccine design. | although esat-6 was originally identified as a strong t cell immunogen in short-term culture filtrate of mtb, and has therefore been a candidate vaccine antigen for many years, recent work has demonstrated that esat-6 is also a virulence factor that mediates pathogenicity of mtb. the studies described in this review suggest that esat-6 secreted by mtb subverts host immunity by manipulating intracellular signaling pathways in macrophages and t cells, which are critical in protection against mtb. ... | 2011 | 22169731 |
| rapidly progressive renal failure-a rare presentation of granulomatous interstitial nephritis due to tuberculosis-case report and review of literature. | granulomatous interstitial nephritis (gin) is a rare manifestation of renal tuberculosis (tb). we report a case of rapidly progressive renal failure (rprf), granulomatous inflammation of cervical lymph node and gin as presenting manifestations of tb. aspiration cytology of cervical lymph node showed granulomatous necrotizing inflammation with acid-fast bacilli (afb). the renal biopsy and urine specimen did not show afb. urine polymerase chain reaction (pcr) for mycobacterium tuberculosis was pos ... | 2011 | 25984199 |
| synthesis and antimycobacterial activity of various 1-(8-quinolinyloxy)-3-piperazinyl(piperidinyl)-5-(4-cyano-3-trifluoromethylphenylamino)-s-triazines. | this study presents the synthesis of novel 1-(8-quinolinyloxy)-3-piperazinyl(piperidinyl)-5-(4-cyano-3-trifluoromethylphenyl amino)-s-triazines. the synthetic route to final piperazinyl s-triazines consists of two nucleophilic substitution reactions of 4-amino-2-trifluoromethylbenzonitrile and 8-hydroxyquinoline with 2,4,6-trichloro-1,3,5-triazine resulting in 2,4-disubstituted-6-chloro-1,3,5-triazine derivatives to introduce the piperazinyl or piperidinyl functionality. the structures of the co ... | 2011 | 24061132 |
| ensuring tuberculosis infection control to support greater involvement of people living with hiv in health care. | health care workers (hcws) in high tb burden countries bear significant risks of being infected with mycobacterium tuberculosis and developing tb disease through their work. in recent years, an increasing number of people living with hiv (plhiv) are taking part in delivering hiv and other health services in resource-limited settings with high tb burden. the greater involvement of plhiv in health service delivery has many beneficial consequences on individuals and health systems, however, the inv ... | 2011 | 24038493 |
| on the pathogenesis of post primary tuberculosis: the role of bronchial obstruction in the pathogenesis of cavities. | mycobacterium tuberculosis produces two distinct types of disease: primary and post primary tuberculosis. we recently reported that post primary tuberculosis begins in immunocompetent adults as an endogenous lipid pneumonia that abruptly undergoes necrosis to produce cavities. obstruction of bronchi by endobronchial tuberculosis is a consistent finding in developing post primary tuberculosis. this paper reports a case of obstructive pneumonia caused by cancer that underwent rapid necrosis to pro ... | 2011 | 22082617 |
| isoniazid mic and katg gene mutations among mycobacterium tuberculosis isolates in northwest of iran. | isoniazid (inh) is one of the main first line drugs used in treatment of tuberculosis and development of resistance against this compound can result in serious problems in treatment procedures. resistance to inh is mediated mainly by mutation in katg gene that is coded for the catalase enzyme. the proportional method for detection of inh-resistance is time consuming due to the slow growth rate of mycobacterium tuberculosis. in this study, we used pcr-rflp approach for screening of common mutatio ... | 2011 | 23493326 |
| einstein contained aerosol pulmonizer (ecap): improved biosafety for multi-drug resistant (mdr) and extensively drug resistant (xdr) mycobacterium tuberculosis aerosol infection studies. | a new apparatus enhances the biosafety of containment (biosafety level 3 [bsl-3]) and provides experimental reproducibility for aerosol infection experiments with mdr and xdr mycobacterium tuberculosis. the methods are generally applicable to the study of airborne pathogens. | 2011 | 23413363 |
| deciphering the structural requirements of nucleoside bisubstrate analogues for inhibition of mbta in mycobacterium tuberculosis: a fb-qsar study and combinatorial library generation for identifying potential hits. | the re-emergence of tuberculosis infections, which are resistant to conventional drug therapy, has steadily risen in the last decade. inhibitors of aryl acid adenylating enzyme known as mbta, involved in siderophore biosynthesis in mycobacterium tuberculosis, are being explored as potential antitubercular agents. the ability to identify fragments that interact with a biological target is a key step in fragment based drug design (fbdd). to expand the boundaries of quantitative structure activity ... | 2011 | 27468106 |
| optimization of structure based virtual screening protocols against thymidine monophosphate kinase inhibitors as antitubercular agents. | thymidine monophosphate kinase from mycobacterium tuberculosis (tmpkmtub ) is an established drug target against tuberculosis. the enzyme tmpkmtub is responsible for the survival of bacterium mtb and required to synthesize an essential building block of the bacterial dna which is thymidine triphosphate (ttp). there are several potent inhibitors available against the target enzyme but the majority are substrate analogues. recently, three dimensional structures of the enzyme tmpkmtub inhibitor com ... | 2011 | 27468105 |
| the use of high performance liquid chromatography to speciate and characterize the epidemiology of mycobacteria. | we evaluated high-performance liquid chromatography (hplc) for species identification of mycobacteria from various clinical specimens in an urban hospital in south korea between january 2005 and december 2009. | 2011 | 24443588 |
| synthesis and antimycobacterial activity of 2-(phenylthio) benzoylarylhydrazone derivatives. | a series of new 2-(phenylthio) benzoylarylhydrazones were synthesized by acid-catalyzed condensation of hydrazide 3 with corresponding aldehydes. the chemical structures of the compounds were elucidated by ft-ir, (1)h-nmr and mass spectra. all newly synthesized compounds were evaluated for their antimycobacterial activities against mycobacterium tuberculosis h37rv using the microplatealamar blue assay (maba). compounds 4f (5-nitro-2-furyl analogue) and 4g (5-nitro-2-thienyl analogue) showed anti ... | 2011 | 24250407 |
| construction and evaluation of an expression vector containing mtb32c (rv0125) of mycobacterium tuberculosis. | expressions of recombinant proteins for different applications are important objectives in molecular biotechnology; however, expression of some recombinant proteins is difficult. several methods have been designed for expression of these proteins. the aim of this study was to construct a vector containing mtb32c fragment of mycobacterium tuberculosis (m.tuberculosis) as a fusion partner in order to improve the expression of fused recombinant proteins. mtb32c was amplified by polymerase chain rea ... | 2011 | 23407610 |
| informatics resources for tuberculosis--towards drug discovery. | integration of biological data on gene sequence, genome annotation, gene expression, metabolic pathways, protein structure, drug target prioritization and selection, has resulted in several online bioinformatics databases and tools for mycobacterium tuberculosis. alongside there has been a growth in the list of cheminformatics databases for small molecules and tools to facilitate drug discovery. in spite of these efforts there is a noticeable lag in the drug discovery process which is an urgent ... | 2012 | 21943870 |
| choroidal biopsy of a patient with aids and systemic tuberculosis: a clinicopathologic case report. | to report a case of mycobacterium tuberculosis choroidal granuloma confirmed by the presence of acid-fast bacilli seen on subretinal biopsy of the choroidal lesion. | 2011 | 25390177 |
| case report: ampiginous chorioretinopathy associated with eales disease in a patient with presumed tuberculosis. | the purpose of this study was to present a case of ampiginous chorioretinopathy followed by eales disease in association with mycobacterium tuberculosis infection. | 2011 | 25390176 |
| rapid identification of mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of dna-streptavidin nanoparticles. | rapid detection of mycobacterium tuberculosis complex (mtbc) is a critical step in controlling tuberculosis (tb). in this study, we used is6110 as the specific identification target to develop a novel hybridization signal amplification method (hsam) for the rapid and direct detection of mtbc from clinical sputum specimens. this system consists of magnetic bead-linked capture probes for target isolation, dextran-based nanoparticles for amplifying the reporter molecule (biotinylated-fitc), and det ... | 2011 | 24031713 |
| rapid detection of isoniazid resistance in mycobacterium tuberculosis by a single multiplex allele-specific polymerase chain reaction assay. | global surveillance has shown that drug resistant (dr) tuberculosis (tb) is widespread. prompt detection of mycobacterium tuberculosis drug resistance is essential for effective control of tb. the most frequent mutations associated with isoniazid (inh) resistance in mycobacterium are substitutions at codons 315 in the katg gene and the maba-inha promoter region (-15). this survey evaluated inh resistant-associated mutations in order to determine rapid detection of tb resistance. | 2011 | 23508230 |
| revisiting the cns tuberculosis with emphasis on giant tuberculomas and introducing the "outer rim excrescence sign". | central nervous system tuberculosis is a leading cause of morbidity and mortality in developing countries with mycobacterium tuberculosis as the main etiological agent. classical imaging findings pose no problem for diagnosis of cns tuberculosis. cases of giant or solitary ring enhancing lesions are increasing which mimic other infectious and noninfectious pathologies. this is a retrospective study of 40 patients (28 male and 12 female) of either surgically or clinically proven cns tuberculosis. ... | 2011 | 24059658 |
| latent mycobacterium tuberculosis infection in liver transplant recipients-controversies in current diagnosis and management. | liver transplantation for end-stage liver disease is increasingly being undertaken in india.(1) routine tuberculin skin testing (tst) for latent mycobacterium tuberculosis infection (ltbi) and isoniazid prophylaxis in tst-positive liver-transplant recipients (ltrs) is recommended(2,3) but seldom implemented worldwide.(4-7) the role of tst-testing and isoniazid prophylaxis in ltrs remains further undefined in high prevalence areas, including india. we describe the burden of ltbi in ltrs; the epid ... | 2011 | 25755308 |
| rapid detection of viable m. tuberculosis directly from sputum by reverse transcriptase pcr targeting 85b gene. | this study was carried out to standardize reverse transcriptase pcr (rt-pcr) targeting 85b gene for the rapid detection of viable mycobacterium tuberculosis (m tuberculosis) from sputum specimens. 100 sputum samples from clinically suspected tuberculosis patients were included in the study. the sputum samples were collected in sterile diethyl pyrocarbonate (depc) treated containers and transported in ice to the laboratory within 2 hours to prevent degradation of rna. the following microbiologica ... | 2011 | 23785865 |
| identification of distant co-evolving residues in antigen 85c from mycobacterium tuberculosis using statistical coupling analysis of the esterase family proteins. | a fundamental goal in cellular signaling is to understand allosteric communication, the process by which signals originating at one site in a protein propagate reliably to affect distant functional sites. the general principles of protein structure that underlie this process remain unknown. statistical coupling analysis (sca) is a statistical technique that uses evolutionary data of a protein family to measure correlation between distant functional sites and suggests allosteric communication. in ... | 2011 | 23554685 |
| in vitro antituberculous activity of thymoquinone, an active principle of nigella sativa. | nigella sativa seed has been used in folk medicine against many ailments including infections. the present study was aimed to investigate activity of thymoquinone, in vitro, against clinical isolates of mycobacterium tuberculosis. | 2014 | 24800349 |
| assessment of the quality of dna extracted by two techniques from mycobacterium tuberculosis for fast molecular identification and genotyping. | we report a comparative study of two dna extraction techniques, thermolysis and chemical lysis (ctab), for molecular identification and genotyping of m. tuberculosis. forty dna samples were subjected to pcr and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping. | 2011 | 24031692 |
| diagnosis of mycobacterium tuberculosis using molecular biology technology. | to present an integrated molecular biology dedicated system for tuberculosis diagnosis. | 2011 | 23569734 |
| incorporating user input in template-based segmentation. | we present a simple and elegant method to incorporate user input in a template-based segmentation method for diseased organs. the user provides a partial segmentation of the organ of interest, which is used to guide the template towards its target. the user also highlights some elements of the background that should be excluded from the final segmentation. we derive by likelihood maximization a registration algorithm from a simple statistical image model in which the user labels are modeled as b ... | 2017 | 26146532 |
| evaluation of nucleic acid probe for rapid identification of mycobacterium tuberculosis complex in extra-pulmonary culture isolates. | patients infected with non-tuberculous mycobacteria (ntm) usually do not respond to conventional anti-tubercular treatment and are misdiagnosed as infection with multi-drug resistant strains of mycobacterium tuberculosis (m.tb) due to lack of correct species identification, particularly in the developing countries like india. one of the challenges faced by clinicians in the treatment of tuberculosis is the absence of an easy, reliable and rapid identification tool that can accurately differentia ... | 2011 | 23785877 |
| tuberculin reactivity in school age children; five-year follow-up in iran. | tuberculosis (tb) is an important infectious disease worldwide. tuberculin skin test (tst) is the standard test for diagnosis of tuberculosis infection; bacillus calmette-guerin (bcg) vaccination at birth has effects on this test. the aim of this study was to determine the prevalence of positive tst cases among 7- to 11-year-old primary school children and also to follow test-positive individuals for a five-year period. | 2011 | 23056762 |
| synthesis, structure, and tuberculostatic activity of dimethyl benzoylcarbonohydrazonodithioates. | new dimethyl benzoylcarbonohydrazonodithioates were obtained by cs2 addition to arylcarboxylic acid hydrazides and methylation of the formed adduct. the new derivatives were tested for their activity against mycobacterium tuberculosis. some compounds exhibited high activity toward sensitive and resistant strains. | 2017 | 26166854 |
| detection of antibodies against 6, 16 and 38 kda antigens of mycobacterium tuberculosis as a rapid test for diagnosis of tuberculosis. | serological assays for diagnosis of tuberculosis (tb) are very attractive because they are inexpensive, non invasive and simple. present study was conducted to evaluate the tuberculosis rapid test device in iran. | 2011 | 25191383 |
| respiratory complications in iranian hospitalized patients with hiv/aids. | the respiratory tract has been the most commonly affected site of illness in hiv-infected patients. the current study was done to identify the frequency of respiratory complications in a consecutive case series of hiv-positive patients in iran. | 2011 | 25191376 |
| evaluation of polymerase chain reaction for diagnosis of "tuberculous pleurisy". | differential diagnosis between tuberculous pleurisy (tbp) and non- tuberculosis pleural effusion represents a critically important clinical problem. in recent years, several noninvasive methods have been found for diagnosis of tuberculous pleurisy. this study aimed to evaluate the value of detection of the genome of mycobacterium tuberculosis (mtb) by polymerase chain reaction (pcr) method for the diagnosis of tuberculous pleurisy and compare the results with those of conventional methods. | 2011 | 25191345 |
| evaluation of a nested-pcr for mycobacterium tuberculosis detection in blood and urine samples. | the polymerase chain reaction (pcr) and its variations, such as the nested-pcr, have been described as promising techniques for rapid diagnosis of tuberculosis (tb). with the aim of evaluating the usefulness of a nested-pcr method on samples of blood and urine of patients suspected of tuberculosis we analyzed 192 clinical samples, using as a molecular target the insertion element is6110 specific of m. tuberculosis genome. nested-pcr method showed higher sensitivity in patients with extrapulmonar ... | 2011 | 24031638 |
| pcr-based evaluation of tuberculous endometritis in infertile women of north india. | tuberculous endometritis is often a diagnostic dilemma for gynecologists in the evaluation of infertility. this study evaluated and compared different diagnostic methods in tuberculous endometritis. | 2011 | 23926494 |
| [tuberculosis]. | tuberculosis (tb) continues to be an important public health problem. currently, 2,100 million people--one third of the world population--are infected by mycobacterium tuberculosis, with an estimated annual rate of 9.4 million new cases, and 440,000 cases of multidrug-resistant (mdr) tb in 2008; furthermore, cases of extensively-resistant (xdr) tb have been detected in 57 countries. while tb cases are constantly declining in industrialized countries, the rates and mortality due to this infection ... | 2011 | 23351514 |
| disseminated tuberculosis mimicking ankylosing spondylitis. | ankylosing spondylitis is a chronic inflammatory disorder affecting mainly the axial skeleton. here we report a case of a man with a clinical suspicion of ankylosing spondylitis but with a persistence of increased inflammatory markers. in this case, (18)f-fdg-pet/ct revealed multiple hypermetabolic lesions in axial skeleton, lymph nodes, and the lung, suggestive of either disseminated tuberculosis or lymphoma. histological analysis of the pulmonary lesion revealed mycobacterium tuberculosis. thi ... | 2011 | 22937440 |
| importance of polymerase chain reaction in diagnosis of pulmonary and extra-pulmonary tuberculosis. | pakistan ranks 8th on the list of 22 high-burden tuberculosis (tb) countries in the world according to the world health organization's (who) global tuberculosis control 2009. including other reasons the main cause is improper and late diagnosis of the disease. pcr may play an important role to control the disease with its rapid, sensitive and specific diagnosis. but in pakistan due to lake of knowledge about this latest technique we are not using this technique appropriately. clinicians still tr ... | 2012 | 22830152 |
| shoulder pain and dysphagia with an unexpected cause. | a 31-year-old female pharmacist of nigerian origin, now resident in london, described 4 months of worsening left-sided subscapular pain despite the use of increasingly potent analgesia. she also described progressive dysphagia, first to solids and later to liquid foods. she remained systemically well, with no associated symptoms and normal physiological observations. in light of raised plasma inflammatory markers and chest radiography demonstrating a widened paraspinal stripe, the patient underw ... | 2011 | 22714602 |
| therapeutic pneumoperitoneum in a patient with pulmonary tuberculosis and persistent fever. | artificial pneumoperitoneum represents a therapeutic technique first applied in the treatment of pulmonary tuberculosis (tb) in prechemotherapy antimycobacterial era. a 25-year-old patient presented with pulmonary tb diagnosed during the 8th month of her pregnancy. she was febrile and in severe clinical condition. an antituberculous regimen of four primary drugs was initiated immediately after the caesarean section. there was no clinical improvement after 3 months despite full drug sensitivity o ... | 2011 | 22714597 |
| primary inoculation skin tuberculosis by accidental needle stick. | a 42-year-old female laboratory worker presented with a left index finger skin lesion after an accidental prick while handling samples of a cultural exam of mycobacterium tuberculosis. surgical excision was performed and pathology analysis revealed a dermic chronic inflammatory process with no granulomas. later, a non-painful lymphadenopathy appeared in the left axilla as well as brownish indurated skin lesions in the lower limbs consistent with erythema nodosum. fine needle aspiration biopsy of ... | 2011 | 22691946 |
| mycobacterium tuberculosis: sometimes, a hidden culprit. | | 2011 | 22689730 |
| primary sjogren's syndrome with tuberculous arthritis of left knee. | a 56-year-old woman presented with recurrent pain and swelling of left knee, raynaud's phenomenon and dry mouth. she was initially diagnosed with primary sjögren's syndrome and was put on prednisone, which substantially relieved her complaints. but 8 months later, pain and swelling of left knee recurred with spiking fever, chills and shortness of breath. escalation of prednisone did not improve the pain and swelling of left knee. ct of chest revealed pulmonary interstitial changes with coexistin ... | 2011 | 22679320 |
| katg (ser 315 thr) gene mutation in isoniazid resistant mycobacterium tuberculosis. | background isoniazid (inh) together with rifampicin (rfp) forms the cornerstone of a short chemotherapy course for tuberculosis (tb) treatment. mutation at codon 315 of katg gene is most prevalent in isoniazid resistant mycobacterium tuberculosis (mtb) and is high in area with high tb incidence. polymerase chain reaction restriction fragment length polymorphism (pcr-rflp) has been found to be a reliable and effective tool for the identification of the specific gene alteration. | 2013 | 22610803 |
| mycobacterium tuberculosis complex genotype diversity and drug resistance profiles in a pediatric population in mexico. | the aim of this study was to determine the frequency of drug resistance and the clonality of genotype patterns in m. tuberculosis clinical isolates from pediatric patients in mexico (n = 90 patients from 19 states; time period-january 2002 to december 2003). pulmonary disease was the most frequent clinical manifestation (71%). children with systemic tuberculosis (tb) were significantly younger compared to patients with localized tb infections (mean 7.7 ± 6.2 years versus 15 ± 3.4 years p = 0.001 ... | 2011 | 22567263 |
| [duties of institutions and heads of health care centers in the area of infection control, information, assessment, registration and financing of benefits provided to tb patients]. | the act on preventing and counteracting infections and infectious diseases in humans effective in poland provides for the duty of the heads of health care outlets and institutions to counteract spreading of tb in units under their management. they are, by all means, responsible for monitoring infections in their respective units, involving development, implementation and monitoring of practical implementation of procedures aiming at limiting dissemination of tb in hospitals and outpatient clinic ... | 2011 | 22533149 |
| high initial bacillary load in patients with pulmonary tuberculosis: an indicator of drug resistant tuberculosis. | this study conducted to evaluate the role of pretreatment bacillary load in predicting multi drug resistance tuberculosis (mdr-tb). sputum smear positive cases of all pulmonary tuberculosis, who received standard short course chemotherapy under direct observation, were studied with pretreatment absolute acid fast bacilli (afb) counts per 100 oil immersion fields and culture-sensitivity assay for mycobacterium tuberculosis. pretreatment count of > or = 850 bacilli per oil immersion field in categ ... | 2010 | 22471192 |
| phospholipase c in beijing strains of mycobacterium tuberculosis. | phospholipase of mycobacterium tuberculosis plays an important role in pathogenesis through breaking up phospholipids and production of diacylglycerol. in this study, we examined the beijing strains of mycobacterium tuberculosis isolated from iranian patients for the genes encoding this enzyme. | 2010 | 22347572 |
| chest radiographic findings in primary pulmonary tuberculosis: observations from high school outbreaks. | to describe the radiographic findings of primary pulmonary tuberculosis (tb) in previously healthy adolescent patients. | 2016 | 21076586 |
| ethambutol resistance of indigenous mycobacterium tuberculosis isolated from human patients. | the present study was conducted to find out the ethambutol resistance pattern of indigenous isolates of mycobacterium tuberculosis from tuberculosis diagnosed human patients. a total of 172 specimens were collected from six different sources and comprised of 84.9% sputum, 10.5% pus and 4.7% bronchial washings. there were 70.9% males and 29.1% females with 84.30% pulmonary and 15.69% extra-pulmonary tuberculosis. the mycobacterium tuberculosis isolates collected from primary culture were further ... | 2010 | 24031587 |
| transmission of multidrug-resistant tuberculosis confirmed by endobronchial ultrasound biopsy of a mediastinal lymph node and dna fingerprinting analysis. | endobronchial ultrasound-guided transbronchial needle aspiration (ebus-tbna) was recently introduced for sampling of mediastinal masses and nodes, for staging of lung cancer, and for diagnosing other benign diseases including tuberculosis (tb). however, ebus-tbna has not been used to date to confirm the presence of multidrug-resistant tb (mdr-tb). we here describe the diagnosis of mdr-tb of a mediastinal lymph node by ebus biopsy. mdr-tb had been transmitted to our patient from her son who had p ... | 2010 | 23168959 |
| percentage of cd4+ and cd8+ t-lymphocytes in blood of tuberculosis patients. | tuberculosis is a fatal infectious disease, mainly caused by mycobacterium tuberculosis. spread of tb is controlled by cell-mediated immunity. purpose of this study was to determine cd4+ and cd8+ t cell percentages in tb patients. | 2012 | 22455293 |
| ranking of qsar models to predict minimal inhibitory concentrations toward mycobacterium tuberculosis for a set of fluoroquinolones. | cp-ann technique was used to build 54 different qsar models. the models were built for three sets (assays) of fluoroquinolones considering their antituberculosis activity and using different technical parameters (dimension of network and number of learning epochs). the models served as a reliable basis for ranking by a new powerful method based on sum of ranking differences (srd). with the applied srd procedure we can find the optimal ones. the best model can be selected easily for the first ass ... | 2010 | 24061804 |
| diagnostic efficacy of adenosine deaminase levels in cerebrospinal fluid in patients of tubercular meningitis: a comparison with pcr for mycobacterium tuberculosis. | the rapid diagnosis of tubercular meningitis (tbm) is fundamental to clinical outcome. the key to diagnosis lies in cerebrospinal fluid (csf) analysis and radiological investigations. there are numerous lacunae in the confirmation of diagnosis of tbm from csf. | 2010 | 25205888 |
| synchronous comparison of mycobacterium tuberculosis epidemiology strains by "miru-vntr" and "miru-vntr and spoligotyping" technique. | molecular epidemiology analyses are frequently used in determining epidemiology of tuberculosis. recently, mycobacterial interspersed repetitive unit variable number tandem repeat (miru-vntr) and spoligotyping has become an important method, as it allows high-through put, discriminatory and reproducible analysis of clinical isolate. the purpose of this study is to compare techniques of "miru-vntr" versus "miru-vntr and spoligotyping" together for study of genetic pattern of mycobacterium tubercu ... | 2010 | 23408229 |
| synthesis and biological evaluation of some new imidazo[1,2-a]pyridines. | a series of new 1-[(2,8-dimethylimidazo[1,2-a]pyridine-3-yl)carbonyl]-4-alkyl/arylthiosemicarbazides, 2-[(2,8-dimethylimidazo[1,2-a]pyridine-3-yl)carbonyl]hydrazono-3-alkyl thiazolidin-4-ones, 2-(2,8-dimethylimidazo[1,2-a]pyridine-3-yl)-5-arylamino-1,3,4-oxadiazoles and 4-alkyl/aryl-2,4-dihydro-5-(2,8-dimethylimidazo[1,2-a]pyridine-3-yl)-3h-1,2,4-triazole-3-thiones were synthesized. the structures of the compounds have been elucidated by ir, 1h nmr, ei mass spectra and elemental analysis. antiba ... | 2010 | 24061731 |
| study of different methods of susceptibility testing of m. tuberculosis. | this communication is on a multi-centre collaborative study carried out by new delhi tuberculosis (ndtb) centre, delhi, to compare the different methods of drug susceptibility testing (dst) of mycobacterium tuberculosis for streptomycin, rifampicin, isoniazid and ethambutol in various accredited laboratories 40 samples (selected by simple random sampling technique) from sputum positive out-patients presenting between december 2004 and january 2005 were collected at new delhi tuberculosis centre ... | 2010 | 22471170 |
| growth kinetics of mycobacterium tuberculosis measured by quantitative resazurin reduction assay: a tool for fitness studies. | we standardized a method to evaluate the growth kinetics of mycobacterium tuberculosis by measuring quantitatively the reduction of resazurin by spectrophotometry. growth curves and the rate of growth of twenty-one m. tuberculosis clinical isolates were determined. the method showed technical simplicity and is inexpensive to assess the fitness of each isolate. | 2010 | 24031495 |
| antimycobacterial susceptibility testing methods for natural products research. | the emergence of multidrug-resistant strains of mycobacterium tuberculosis underscores the need of continuous developments on new and efficient methods to determine the susceptibility of isolates of m. tuberculosis in the search for novel antimicrobial agents. natural products constitute an important source of new drugs, but design and implementation of antimycobacterial susceptibility testing methods are necessary for evaluate the different extracts and compounds. a number of biological assay m ... | 2010 | 24031490 |
| diagnosis of gastrointestinal tuberculosis: using cytomorphological, microbiological, immunological and molecular techniques - a study from central india. | the present study included three groups: (a) age and gender matched control (n=24) with no previous signs of m. tuberculosis complex (mtbc) infection, (b) patients (n=28) diagnosed with gastro-intestinal tb (gitb), (c) patients (n=50) with clinical and histo-pathological signs of gitb, but were culture and afb negative. real time assay performed using fluorescence resonance energy transfer hybridization probes showed a positivity index of 36 % in group c, i.e. 18 were found reactive from the tot ... | 2010 | 23105903 |
| new 5-modified pyrimidine nucleoside inhibitors of mycobacterial growth. | the who has declared tuberculosis (tb) a global health emergency. therefore, there is an urgent need to discover and develop new anti-tb drugs. here we report on a new category of 5-substituted pyrimidine nucleosides as potent inhibitors of myco-bacterium tuberculosis growth in vitro. a series of 2'-deoxy-, 3'-azido-2',3'-dideoxy-, and 3'-amino-2',3'-dideoxypyrimidine nucleoside analogues bearing lengthy flexible alkyloxymethyl substituents exhibited marked inhibitory activity against m. tubercu ... | 2010 | 22649636 |
| novel drug delivery systems: desired feat for tuberculosis. | tuberculosis has claimed its victims throughout much of known human history and is currently the most devastating human bacterial disease. the ability to infect human population on a global scale, combined with the widespread emergence of multi-drug resistant strains, has led to the placement of mycobacterium tuberculosis on the national institute of allergy and infectious diseases (niaid) list of biodefence and emerging infectious disease threats agents. the resurgence of interest in tuberculos ... | 2010 | 22247841 |
| drug susceptibility profiles of mycobacterium tuberculosis isolates at mangalore. | tuberculosis (tb) remains one of the major causes of death from a single infectious agent worldwide. its resurgence in 1990s is primarily due to co-infection with hiv and the emergence of multi-drug-resistant (mdr) strains. our objectives in this study were demonstration and grading of acid-fast bacilli in smears from sputum specimens of clinically newly diagnosed pulmonary tb patients, isolation of the organism, speciation and drug susceptibility testing of mycobacterium tuberculosis isolates t ... | 2010 | 22569321 |
| [low-intensity laser therapy in the prophylactic treatment of tuberculin hyperergic children]. | the results of sanatorium treatment were analyzed in 156 children and adolescents aged 11-17 years at high risk for tuberculosis. all the patients had been long infected with mycobacterium tuberculosis with hyperergic tuberculin sensitivity; they had comorbidity and were susceptible to frequent respiratory diseases. a course of percutaneous low-intensity laser irradiation was incorporated as a new method into a complex of prophylactic treatment. the time course of changes in tuberculin sensitivi ... | 2010 | 27534057 |
| [the current aspects of diagnosis and differential diagnosis of complications after bcg vaccination]. | whether the diagnosis of bcg complications might be verified by molecular genetic methods, such as polymerase chain reaction (pcr) and sequencing, was studied. pcr could identify mycobacterium tuberculosis as m. bovis strain bcg and increase the detection rate of m. tuberculosis by 38% as compared with traditional microbiological assays, which provides a means of recommending the method to verify the diagnosis of bcg complications. | 2010 | 27534052 |
| [simulation of chronic tuberculosis infection in mice by intraperitoneal inoculation and the testing of aminoguanidine-induced activation of rpf gene/deletion mutants of the h37rv strain]. | intraperitoneal inoculation of mice with the virulent strain h37rv of mycobacterium tuberculosis led to the development of chronic tuberculosis for a long time. the developed model of chronic tuberculosis was used to study tuberculous infection caused by rpf gene knockout mutants of the h37rv strain. these mutant strains were unable to produce proteins that determined the transition of quies- cent to active forms of the pathogen of tuberculosis in vitro. unlike the wild-type m. tuberculosis h37r ... | 2010 | 27534027 |
| [the sensitivity and specificity of the molecular genotype mtbdrpl us assay (hain lifescience gmbh, germany) for rapid diagnosis of drug susceptibility in mycobacterium tuberculosis on sputum specimens]. | the efficiency of the molecular genotype mtbdrplus (hain lifescience gmbh, germany) assay was evaluated for the rapid diagnosis of rifampicin and isoniazid susceptibility in mycobacterium tuberculosis (mbt) on 168 sputum specimens from patients with pulmonary tuberculosis in the samara region. the interpretable results from the molecular genetic assay were obtained for 91.7% of the specimens with the rates significantly higher in the sputum specimens graded 2+ and 3+ than in those graded 1+. the ... | 2010 | 27534023 |
| [effect of liquid probiotics on the growth of mycobacterium tuberculosis in vitro]. | the mycobacterium tuberculosis (mbt) culture isolated from the sputum of 25 patients was experimentally studied. the probiotics were tested for their bacterium-neutralizing effect on a dense luwenstein-jensen medium, by using the absolute concentration method. the nature of their bactericidal and bacteriostatic effects was assessed from the presence and intensity of mbt growth on day 21 after inoculation. in most cases, the probiotics exerted an inhibitory effect on mbt growth. the latter was co ... | 2010 | 27534022 |
| [clinical and x-ray changes in patients with new-onset multidrug-resistant mycobacterium pulmonary tuberculosis in the early use of a reserve chemotherapy regimen according to the tb-biochip test system data]. | clinical and x-ray changes were comparatively studied in new-onset tuberculosis patients excreting susceptible mycobacterium tuberculosis (mbt) and mutidrug resistant (mdr) mbt. eighty tuberculosis patients who had not received antituberculous drugs were followed up. before chemotherapy, sputum was tested in all cases, by using the tb-biochip system, to determine drug susceptibility of mbt. according to the results of microchip diagnosis, the patients were divided into 2 groups: 1) patients with ... | 2010 | 27534019 |
| [immediate results of surgical treatment in destructive pulmonary tuberculosis patients excreting extreme drug-resistant mbt]. | the authors analyzed the immediate results of surgical treatment in 110 patients with destructive pulmonary tuberculosis with extreme drug resistance in mycobacterium tuberculosis. out of the 110 patients, 118 were discharged from hospital and 2 continued treatment at the study. a complete clinical effect (abacillation and no decay cavities) was achieved in 96 (88.9%) patients; improvement (abacillation with the decay cavity being preserved in the contralateral lung) was seen in 5 (4.6%) patient ... | 2010 | 27529953 |
| [circulation of multidrug-resistant tuberculosis pathogen strains in the republic of karelia]. | one hundred and sixteen drug-resistant (dr) mycobacterium tuberculosis (mbt) strains and 127 multidrug-resistant (mdr) mbt ones isolated from first detected patients with tuberculosis were studied. molecular genetic methods were used to study 119 strains (54 mdr, 40 dr, and 25 polyresistant (pr) strains. among the mdr strains, the high in vitro growth rate and intensity was seen 10 times more frequently than those among the strains with preserved drug resistance. the absolute majority (90.9%) of ... | 2010 | 27529945 |