| heterologous expression of an entamoeba histolytica chitin synthase in saccharomyces cerevisiae. | chitin in the cyst wall of entamoeba histolytica is made by two chitin synthases (chs), one of which is unique (ehchs-1) and one of which resembles those of insects and nematodes (ehchs-2). ehchs-1 is deposited chitin in the lateral wall of transformed saccharomyces cerevisiae chs mutants, independent of accessory proteins (chs4p to chs7p) required by yeast chs3p. | 2006 | 16400183 |
| blackhead disease (histomoniasis) in poultry: a critical review. | after its discovery in 1893 in rhode island, blackhead disease was reported across the continent and soon in many other countries. it decimated the turkey industry in new england and followed production like a faithful shadow. blackhead disease causes high mortality in turkeys, sometimes approaching 100% of a flock. in chickens, the mortality may be 10%-20% with high morbidity, although many outbreaks pass unnoticed. early workers identified histomonas meleagridis, a protozoan related to entamoe ... | 2005 | 16404985 |
| signaling through the phosphatidylinositol 3-kinase regulates mechanotaxis induced by local low magnetic forces in entamoeba histolytica. | in micro-organisms, as well as in metazoan cells, cellular polarization and directed migration are finely regulated by external stimuli, including mechanical stresses. the mechanisms sustaining the transduction of such external stresses into intracellular biochemical signals remain mainly unknown. using an external magnetic tip, we generated a magnetic field gradient that allows migration analysis of cells submitted to local low-intensity magnetic forces (50 pn). we applied our system to the amo ... | 2007 | 16406381 |
| rho signaling in entamoeba histolytica modulates actomyosin-dependent activities stimulated during invasive behavior. | interaction of entamoeba histolytica trophozoites with target cells and substrates activates signaling pathways in the parasite. phosphorylation cascades triggered by phospho-inositide and adenyl-cyclase-dependent pathways modulate reorganization of the actin cytoskeleton to form structures that facilitate adhesion. in contrast, little is known about participation of rho proteins and rho signaling in actin rearrangements. we report here the in vivo expression of at least one rho protein in troph ... | 2006 | 16421927 |
| entamoeba histolytica infection in children and protection from subsequent amebiasis. | the contribution of amebiasis to the burden of diarrheal disease in children and the degree to which immunity is acquired from natural infection were assessed in a 4-year prospective observational study of 289 preschool children in an urban slum in dhaka, bangladesh. entamoeba histolytica infection was detected at least once in 80%, and repeat infection in 53%, of the children who completed 4 years of observation. annually there were 0.09 episodes/child of e. histolytica-associated diarrhea and ... | 2006 | 16428733 |
| human tumor necrosis factor is a chemoattractant for the parasite entamoeba histolytica. | in an analysis of the molecular factors triggering amebiasis, we investigated the chemotaxis of entamoeba histolytica toward tumor necrosis factor (tnf) in vitro, using quantitative imaging techniques. our findings enabled us to propose a hitherto unknown role for tnf as a chemokinetic and chemoattractant agent for this parasite. | 2006 | 16428794 |
| identification and functional characterization of ehclc-a, an entamoeba histolytica clc chloride channel located at plasma membrane. | clc chloride channels perform a wide variety of physiological functions and they had been characterized in animals, yeast, plants and bacteria but not in protozoa. by blast search we found in entamoeba histolytica, the protozoan responsible for human amoebiasis, two genes (ehclc-a and ehclc-b) encoding for putative polypeptides with 25-30% identity to clc chloride channels of several organisms. reverse transcription polymerase chain reaction (rt-pcr) experiments showed that both genes are transc ... | 2006 | 16430698 |
| prevalence of blastocystis hominis infection in asymptomatic individuals from bangkok, thailand. | fresh stool examination was performed from 2,230 participants who enrolled in annual check-up programs of the faculty of medical technology, mahidol university in 1999-2000 and 2004. in this study, blastocystis hominis infection was diagnosed by culturing in jones' media. a total of 21% of fecal specimens (in 1999-2000) and 13.7% (in 2004) were positive for b. hominis. the vacuolated form was the predominant form found in culture solution after 48 hours of incubation. the distribution of infecti ... | 2005 | 16438173 |
| current infection rate of giardia lamblia in two provinces of thailand. | the aim of this study was to survey for the current rate of giardia lamblia infection in three different districts in two provinces of thailand, surin and samut sakhon, in march 2002, october 2003, and march 2004. two districts are rural areas and another is sub-urban. volunteers of any age from surin province and children aged between 5 to 7 years old from three secondary schools in samut sakhon were enrolled for the stool examination. the method used was stool examination by both simple smear ... | 2005 | 16438174 |
| dioxo- and oxovanadium(v) complexes of thiohydrazone ons donor ligands: synthesis, characterization, reactivity, and antiamoebic activity. | as a contribution to the development of novel vanadium complexes with pharmacologically interesting properties, two neutral dioxovanadium(v) complexes [vo2(hpydx-sbdt)] (1) and [vo2(hpydx-smdt)] (3) [h2pydx-sbdt (i) and h2pydx-smdt (ii) are the schiff bases derived from pyridoxal and s-benzyl- or s-methyldithiocarbazate] have been synthesized by the reaction of [vo(acac)2] and the potassium salts of the ligands in methanol followed by aerial oxidation. heating of the methanolic solutions of thes ... | 2006 | 16441138 |
| kikuchi fujimoto disease secondary to entamoeba histolytica: case report. | kikuchi fujimoto disease (kfd) is a rare histiocytic necrotizing lymphadenitis which has a benign self-limiting clinical course. its origin is unknown, but an abnormal autoimmune reaction has been suggested and infection is often considered to be an inciting agent. a 50-year-old man presented with fever, malaise, fatigue and sweat of 7 days duration, and diarrhea for 2 days. physical examination revealed five mobile and painless cervical adenopathies. entamoeba histolytica trophozoites and cysts ... | 2006 | 16442163 |
| identification and characterization of ehabc a1, an entamoeba histolytica group a abc transporter with similarity to ced-7. | | 2006 | 16442643 |
| the anti-amoebic activity of some medicinal plants used by aids patients in southern thailand. | the anti-amoebic activities of chloroform, methanol and water extracts from 12 thai medicinal plants (39 extracts) commonly used by aids patients in southern thailand were screened, at a concentration of 1,000 microg/ml, against entamoeba histolytica strain hth-56:mutm and strain hm1:imss growing in vitro. the extracts were incubated with 2x10(5) e. histolytica trophozoites/ml of medium at 37 degrees c under anaerobic conditions for 24 h. the cultures were examined with an inverted microscope an ... | 2006 | 16447069 |
| malaria and intestinal parasitosis among children presenting to the paediatric centre in sana'a, yemen. | we studied the profile of malaria and intestinal parasitosis among children presenting to the paediatric health centre in sana'a from january 1998 to december 2000. in stool samples from 9014 children, ascaris lumbricoides, entamoeba histolytica, giardia lamblia and trichuris trichiura were the most common. infection with parasites of direct life-cycle were similar in boys and girls. schistosome infection was significantly higher in boys than girls, but girls were more infected with ascariasis. ... | 2003 | 16450536 |
| the frequency of sister chromatid exchanges in cultured human peripheral blood lymphocyte treated with metronidazole in vitro. | 5-nitroimidazoles are a group of antiprotozoal and antibacterial agents. thanks to their antimicrobial activity, these chemotherapeutic agents inhibit the growth of both anaerobic bacteria and certain anaerobic protozoa. one of the useful drugs used in the treatment of infections caused by trichomonas vaginalis, entamoeba histolytica, and giardia lamblia is metronidazole (mtz). the mutagenicity of metronidazole [1-(hydroxyethyl)-2-methyl-5-nitroimidazole] has been previously shown in different p ... | 2006 | 16455592 |
| entamoeba histolytica: comparison of the role of receptors and filamentous actin among various endocytic processes. | entamoeba histolytica is the causative agent of amoebic dysentery. uptake of iron is critical for e. histolytica growth and iron-bound human transferrin (holo-transferrin) has been shown to serve as an iron source in vitro. although a transferrin-binding protein has been identified in e. histolytica, the mechanism by which this iron source is taken up by this pathogen is not well understood. to gain insight into this process, the uptake of fluorescent-dextran, -holo-transferrin, and human red bl ... | 2006 | 16458294 |
| synthesis, characterisation, reactivity and in vitro antiamoebic activity of hydrazone based oxovanadium(iv), oxovanadium(v) and mu-bis(oxo)bis{oxovanadium(v)} complexes. | binuclear, mu-bis(oxo)bis{oxovanadium(v)} complexes [(vol)2(mu-o)2](2 and 7)(where hl are the hydrazones hacpy-nah i or hacpy-fah ii; acpy = 2-acetylpyridine, nah = nicotinic acid hydrazide and fah = 2-furoic acid hydrazide) were prepared by the reaction of [vo(acac)2] and the ligands in methanol followed by aerial oxidation. the paramagnetic intermediate complexes [vo(acac)(acpy-nah)](1) and [vo(acac)(acpy-fah)](6) have also been isolated. treatment of [vo(acac)(acpy-nah)] and [vo(acac)(acpy-fa ... | 2006 | 16462954 |
| a new protein superfamily includes two novel 3-methyladenine dna glycosylases from bacillus cereus, alkc and alkd. | soil bacteria are heavily exposed to environmental methylating agents such as methylchloride and may have special requirements for repair of alkylation damage on dna. we have used functional complementation of an escherichia coli tag alka mutant to screen for 3-methyladenine dna glycosylase genes in genomic libraries of the soil bacterium bacillus cereus. three genes were recovered: alkc, alkd and alke. the amino acid sequence of alke is homologous to the e. coli alka sequence. alkc and alkd rep ... | 2006 | 16468998 |
| co-ordinate but disproportionate activation of apoptotic, regenerative and inflammatory pathways characterizes the liver response to acute amebic infection. | the liver has the remarkable ability to respond to injury with repair and regeneration. the protozoan parasite entamoeba histolytica is the major cause of liver abscess worldwide. we report a transcriptional analysis of the response of mouse liver to e. histolytica infection, the first study looking at acute liver infection by a non-viral pathogen. focusing on early time points, we identified 764 genes with altered transcriptional levels in amebic liver abscess. the response to infection is rapi ... | 2006 | 16469061 |
| phylogenetic positions of several amitochondriate protozoa--evidence from phylogenetic analysis of dna topoisomerase ii. | several groups of parasitic protozoa, as represented by giardia, trichomonas, entamoeba and microsporida, were once widely considered to be the most primitive extant eukaryotic group--archezoa. the main evidence for this is their 'lacking mitochondria' and possessing some other primitive features between prokaryotes and eukaryotes, and being basal to all eukaryotes with mitochondria in phylogenies inferred from many molecules. some authors even proposed that these organisms diverged before the e ... | 2005 | 16483135 |
| structural differences in triosephosphate isomerase from different species and discovery of a multitrypanosomatid inhibitor. | we examined the interfaces of homodimeric triosephosphate isomerase (tim) from eight different species. the crystal structures of the enzymes showed that a portion of the interface is markedly similar in tims from trypanosoma cruzi (tctim), trypanosoma brucei, and leishmania mexicana and significantly different from that of tims from human, yeast, chicken, plasmodium falciparum, and entamoeba histolytica. since this interfacial region is central in the stability of tctim, we hypothesized that it ... | 2006 | 16489748 |
| important zoonotic intestinal protozoan parasites in asia. | intestinal protozoa are increasingly being studied because of their association with acute and chronic diarrhoea in immunocompromised as well as immunocompetent patients. various community outbreaks due to contamination of water or food with these protozoa have further highlighted their importance in public health. among these important pathogens are giardia duodenalis, entamoeba histolytica, cryptosporidium parvum, cyclospora cayetanensis, isospora belli, and microsporidia. except for the cyst- ... | 2004 | 16493397 |
| proteinases in naegleria fowleri (strain nf3), a pathogenic amoeba: a preliminary study. | naegleria fowleri is a free-living amoeba, known as a causative agent for a fatal disease of the central nervous system (cns) in man such as primary amoebic meningoencephalitis (pam). factors contributing to its pathogenicity and its distribution in the environment have been investigated by previous researchers. in case of its pathogenicity, several enzymes such as phospolipase a and sphingomyelinase, have been proposed to probably act as aggressors in promoting pam but no study so far have been ... | 2004 | 16493399 |
| synthesis, characterization and antiamoebic activity of 1-(thiazolo[4,5-b]quinoxaline-2-yl)-3-phenyl-2-pyrazoline derivatives. | a new series of 1-n-thiocarboxamide-3-phenyl-2-pyrazolines 1-6 was synthesized by cyclization of different mannich bases with unsubstituted thiosemicarbazide. the reaction of cyclized pyrazoline derivatives 1-6 with 2,3-dichloroquinoxaline afforded the title compounds 7-12. the structures of the new compounds were confirmed by elemental analyses as well as (1)h, (13)c nmr, ir and electronic spectral data. the hm1:imss strain of entamoeba histolytica parasite was cultured in vitro and the sensiti ... | 2006 | 16495051 |
| colonic amoebic abscess mimicking carcinoma of the colon. | amoebiasis is an uncommon disease in developed countries. its clinical presentation can be variable and non-specific, and the diagnosis can be easily overlooked. we report a case of colonic amoebic abscess mimicking advanced colonic cancer with acute intestinal obstruction and liver metastasis. the presentation, diagnosis, and treatment of amoebiasis are also reviewed. | 2006 | 16495594 |
| pleiotropic phenotype in entamoeba histolytica overexpressing dna methyltransferase (ehmeth). | the protozoan parasite entamoeba histolytica expresses a cytosine-5 dna methyltransferase (ehmeth) that belongs to the dnmt2 proteins family. the biological function of members of the dnmt2 family is unknown. constitutive overexpression of ehmeth resulted in a pleiotropic phenotype that includes accumulation of multinucleated cells, upregulation of heat shock protein 70 (hsp70) expression and resistance to oxidative stress. this pleiotropic phenotype suggests that ehmeth plays an important role ... | 2006 | 16497397 |
| epidemiology, etiology and pathophysiology of traveler's diarrhea. | traveler's diarrhea (td) is the most frequent health problem in travelers to developing countries. several personal and environmental risk factors are at the basis of td acquisition and are discussed in this paper. td is caused by a wide range of infectious organisms, etec and eaec bacteria strains being the main enteropathogens incriminated in td. other causative bacteria are: shigella spp., campylobacter spp., vibrio spp., aeromonas spp., salmonella spp., and plesiomonas spp. parasite species ... | 2006 | 16498258 |
| crystallization and preliminary crystallographic analysis of calcium-binding protein-2 from entamoeba histolytica and its complexes with strontium and the iq1 motif of myosin v. | calcium plays a pivotal role in the pathogenesis of amoebiasis, a major disease caused by entamoeba histolytica. two domains with four canonical ef-hand-containing calcium-binding proteins (cabps) have been identified from e. histolytica. even though they have very high sequence similarity, these bind to different target proteins in a ca2+-dependent manner, leading to different functional pathways. calcium-binding protein-2 (ehcabp2) crystals were grown using mpd as a precipitant. the crystals b ... | 2005 | 16511057 |
| cloning and partial characterization of entamoeba histolytica ptpases. | reversible protein tyrosine phosphorylation is an essential signal transduction mechanism that regulates cell growth, differentiation, mobility, metabolism, and survival. two genes coding for protein tyrosine phophatases, designed ehptpa and ehptpb, were cloned from entamoeba histolytica. ehptpa and ehptpb proteins showed amino acid sequence identity of 37%, both ehptpases showed similarity with dictyostelium discoideum and vertebrate trasmembranal ptpases. mrna levels of ehptpa gene are up-regu ... | 2006 | 16513090 |
| entamoeba histolytica: computational identification of putative microrna candidates. | micrornas are an abundant class of approximately 20-24-nucleotide noncoding rnas that control the expression of several genes at the post-transcriptional level, regulating several important biochemical pathways. micrornas have been identified in many vertebrates including humans, and also in flies, nematodes, plants, and viruses. in the present work we have identified seventeen putative candidate micrornas in the pathogenic parasite entamoeba histolytica using a bioinformatic approach. putative ... | 2006 | 16515787 |
| emerging opportunistic protozoa and intestinal pathogenic protozoal infestation profile in children of western nepal. | intestinal parasitic infestation continues to be of public health importance in many tropical and subtropical countries for their high prevalence and effects on the morbidity in the population. this 5-year hospital-based retrospective analysis was aimed to find out the intestinal protozoal parasitic profile in 1790 pre-school and school-going children visiting the hospital with gastrointestinal illness. giardia lamblia was the most prevalent pathogenic protozoan intestinal parasite (73.4%), foll ... | 2005 | 16519082 |
| cognitive effects of diarrhea, malnutrition, and entamoeba histolytica infection on school age children in dhaka, bangladesh. | cognitive function was assessed in 191 bangladeshi children 6-9 years of age using verbal and nonverbal tests. these scores were added to a health surveillance database that was compiled over the four previous years that includes incidence of diarrhea and entamoeba histolytica infection and nutritional status. the associations of diarrhea, malnutrition, and social factors with cognitive scores were analyzed statistically, and associations between diarrhea and test scores were controlled for the ... | 2006 | 16525109 |
| a novel saposin-like protein of entamoeba histolytica with membrane-fusogenic activity. | amoebapores, the pore-forming proteins of entamoeba histolytica, are major pathogenicity factors of the parasite. upon a comprehensive survey in the recently completed genome data sets for the protozoon, we identified in addition to the three amoebapore genes, 16 genes which are constitutively expressed and code for structurally similar proteins, all belonging to the family of saposin-like proteins. here, we recombinantly expressed in bacteria a defined single entity of this expansive amoebic pr ... | 2006 | 16529828 |
| dna methylation and targeting of line retrotransposons in entamoeba histolytica and entamoeba invadens. | in this study, we have isolated by affinity chromatography, using anti-m5c antibody as a ligand, a dna encoding reverse transcriptase of line retrotransposon (rt line) in both entamoeba invadens and entamoeba histolytica. rt line transcripts were detected in e. histolytica but were absent from e. invadens. the methylation status of genomic copies of e. invadens rt line was confirmed by bisulfite analysis. in contrast, all the genomic copies of the e. histolytica rt line analyzed in this study we ... | 2006 | 16530279 |
| antisense inhibition of entamoeba histolytica cysteine proteases inhibits colonic mucus degradation. | the exact role entamoeba histolytica cysteine proteases play in overcoming the colonic mucus barrier, as a prerequisite to epithelial cell disruption, is not known. herein, we determined whether e histolytica trophozoites expressing the antisense transcript to cysteine protease 5 (ehcp5) could degrade colonic mucin and destroy epithelial cells. | 2006 | 16530514 |
| two families of rep-like genes that probably originated by interspecies recombination are represented in viral, plasmid, bacterial, and parasitic protozoan genomes. | two families of genes related to, and including, rolling circle replication initiator protein (rep) genes were defined by sequence similarity and by evidence of intergene family recombination. the rep genes of circoviruses were the best characterized members of the "recrep1 family." other members of the recrep1 family were rep-like genes found in the genomes of the canarypox virus, entamoeba histolytica, and giardia duodenalis and in a plasmid, p4m, from the gram-positive bacterium, bifidobacter ... | 2006 | 16531508 |
| [prevalence of intestinal parasites in children of yukpa ethnia in toromo, zulia state, venezuela]. | yupkpa community in perijá sierra does not have drinking water or sewage elimination systems. thus it becomes a propitious place for infection with intestinal parasites. | 2006 | 16532165 |
| prevalence of human immunodeficiency virus and sexually transmitted infections and risky sexual behaviors among men visiting gay bathhouses in taiwan. | this study aimed to investigate the prevalence of human immunodeficiency virus (hiv) and sexually transmitted infections (stis) and predictors of risky sexual behaviors among men visiting gay bathhouses. | 2006 | 16543861 |
| cyclooctadiene ru(ii) complexes of thiophene-2-carboxaldehyde-derived thiosemicarbazones: synthesis, characterization and antiamoebic activity. | thiosemicarbazones (tsc) 1-10 were synthesized by condensing substituted thiosemicarbazide with thiophene-2-carboxaldehyde. these thiosemicarbazones were further reacted with [ru(eta4-c8h12)(ch3cn)2cl2] to form complexes of the type [ru(eta4-c8h12)(tsc)cl2] 1a-10a. thiosemicarbazones exhibited antiamoebic activity in the range ic50=1.09-5.42 microm. in vitro assessment of antiamoebic activity indicated that the thiosemicarbazones 3, ic50=1.67 microm, 4, ic50=1.11 microm and 6, ic50=1.09 microm s ... | 2006 | 16545496 |
| entamoeba histolytica: differences in phagosome acidification and degradation between attenuated and virulent strains. | phagocytosis is the important virulent determinant of the enteric protozoan parasite entamoeba histolytica. we compared the kinetics of phagosome maturation of attenuated and highly-virulent strains of e. histolytica using video microscopy. phagosomes of attenuated strains were acidified rapidly within 2 min after phagosome formation (at the rate of 0.96 ph/min), persisted at ph 4.46+/-0.13, and degraded ingested gfp-leishmania very efficiently (90-94% gfp fluorescence was lost in 30 min), while ... | 2006 | 16546173 |
| a rare cause of colonic stricture: amebiasis. | a 41-year-old man presenting with lower abdominal pain, constipation, abdominal distention, fever (37.5 degrees c) and fatigue was evaluated, and a mass localized to the left lower abdomen was identified. radiographic and colonoscopic examination revealed a stricture 10 cm in length localized to the sigmoid-descending colon junction. the diagnosis of amebiasis was confirmed by histological examination of a biopsy specimen taken from the stricture and stool examination. one month after the initia ... | 2005 | 16547856 |
| impact of intestinal colonization and invasion on the entamoeba histolytica transcriptome. | a genome-wide transcriptional analysis of entamoeba histolytica was performed on trophozoites isolated from the colon of six infected mice and from in vitro culture. an affymetrix platform gene expression array was designed for this analysis that included probe sets for 9435 open reading frames (orfs) and 9066 5' and 3' flanking regions. transcripts were detected for > 80% of all orfs. a total of 523 transcripts (5.2% of all e. histolytica genes) were significantly changed in amebae isolated fro ... | 2006 | 16569449 |
| drug resistance in amoebiasis. | amoebiasis caused by entamoeba histolytica, is a major public health problem in developing countries. morphologically similar e. dispar is non pathogenic. because of the redefinition of e. histolytica and e. dispar, and the limited number of antiamoebic drugs available, a new approach to treat such individuals is necessary. the cost of treating asymptomatic individuals is highly exorbitant and not justifiable. the indiscriminate use of antiamoebic drugs can result in increased minimum inhibitory ... | 2006 | 16575108 |
| prevalence of intestinal parasitic infections in the islamic republic of iran. | a national survey of the prevalence of intestinal parasitic infections in the islamic republic of iran was made on a random sample of families covered by local health centres affiliated to the medical universities. out of 53,995 people aged 2+ years, from 12,495 families (0.1% of all families in 1999), 45,128 stool samples were analysed by formalin-ether precipitation. intestinal parasitic infections were found in 19.3% of the study population (19.7% male, 19.1% female). giardia lamblia (10.9%), ... | 2005 | 16602457 |
| cytotoxicity and in vitro susceptibility of entamoeba histolytica to morinda morindoides leaf extracts and its isolated constituents. | an aqueous decotion (dried extract), an 80% meoh extract from morinda morindoides leaves, and 10 flavonoids and 4 iridoids isolated from the 80% meoh extract were evaluated in vitro for their potential antiamoebic activity and their cytotoxic effect against mt-4 cells. results indicated that the aqueous decoction and the 80% meoh extract exhibited an interesting antiamoebic activity with ic(50) values of 3.1 +/- 1.7 and 1.7 +/- 0.6 microg/ml, respectively. apigenin-7-o-glucoside and luteolin-7-o ... | 2006 | 16603327 |
| detection of protozoal parasites in the stools of diarrhoeic patients using different techniques. | a total of 300 children were selected. a total of 180 apparently healthy children were selected. each child was given a numerical number and subjected to a medical questionnaire. the rate of infection by the enteric protozoa detected by the four techniques revealed that g. lamblia reached 11.0% by concentration-sedimentation method, 10.3% by sheather's concentration method and 7.0% (cysts) and 1.7% (vegetative) by direct wet smear. e. histolytica was 3.3% & 3.7% by the two concentration methods ... | 2006 | 16605096 |
| patients with gastrointestinal complains due to enteric parasites, with reference to entamoeba histolytica/dispar as dected by elisa e. histolytica adhesion in stool. | a total of 210 patients with gastrointestinal troubles, of both sex and a mean age of 32 +/- 6.1 years, selected from the outpatient's clinics of al-azhar university hospitals. 115 (54.76%) had dysentery, 95 (45.23%) did not have dysentery, 15 (14%) suffered flatulence, 20 (9.52%) had epi-gastric pain, 19 (9.05%) had vague abdominal pain, 5 vomiting (5.2%) and 10 (4.9%) had fever. two symptoms were in 29 (13.81%) patients and three symptoms in 12 (5.71%). of the 210 patients, 20 (9.9%) had helmi ... | 2006 | 16605100 |
| naegleria fowleri: a free-living highly pathogenic amoeba contains trypanothione/trypanothione reductase and glutathione/glutathione reductase systems. | this paper presents definitive data showing that the thiol-bimane compound isolated and purified by hplc from naegleria fowleri trophozoites unequivocally corresponds by matrix assisted laser-desorption ionization-time-of-flight ms, to the characteristic monoprotonated ion of trypanothione-(bimane)(2) [m(+)h(+)] of m/z 1104.57 and to the trypanothione-(bimane) of m/z 914.46. the trypanothione disulfide t(s)(2) was also found to have a molecular ion of m/z 723.37. additionally hplc demonstrated t ... | 2006 | 16620809 |
| the cyst wall of entamoeba invadens contains chitosan (deacetylated chitin). | the cyst wall of entamoeba invadens (ei), a model for the human pathogen entamoeba histolytica, contains chitin, which is a homopolymer of beta-1, 4-linked n-acetyl-glucosamine (glcnac). in fungi and in bacteria that make nodulation factors, chitin deacetylases make chitosan, which is a mixture of glcnac and glucosamine and so has a positive charge. the activity of an ei chitin deacetylase was revealed by a 3-4-fold increase in released glcnac when deproteinated cyst walls were chemically acetyl ... | 2006 | 16621070 |
| overdiagnosis of entamoeba histolytica and entamoeba dispar in nicaragua: a microscopic, triage parasite panel and pcr study. | based on stool microscopy, an e. histolytica/e. dispar prevalence of 18.6% was found in león, nicaragua about 10 years ago. since then, new non-microscopic methods have been developed to discriminate between pathogenic e. histolytica and nonpathogenic e. dispar. the main objectives of the present study were to evaluate the true prevalence of e. histolytica among individuals with diarrhea and to assess the diagnostic procedures carried out at the health center level. | 2006 | 16624654 |
| structure of alcohol dehydrogenase from entamoeba histolytica. | the structure of the apo form of alcohol dehydrogenase from a single-cell eukaryotic source, entamoeba histolytica, has been determined at 1.8 a. to date, bacterial and archeal alcohol dehydrogenases, which are biologically active as tetramers, have crystallized with tetramers in the asymmetric unit. however, the current structure has one independent dimer per asymmetric unit and the full tetramer is generated by application of the crystallographic twofold symmetry element. this structure reveal ... | 2006 | 16627948 |
| entamoeba histolytica: ultrastructure of the chromosomes and the mitotic spindle. | we have analyzed by transmission electron microscopy the mitotic process of entamoeba histolytica trophozoites in an asynchronous population of axenically cultured parasites. our observations showed that nuclear microtubules, initially located at random in the karyosome during prophase, formed in subsequent stages a mitotic spindle closely related to the nuclear membrane at the polar regions of dividing nuclei. in late prophase and in anaphase, chromosomes appeared as dense bodies 0.1-0.5 microm ... | 2006 | 16631745 |
| entamoeba histolytica: inflammatory process during amoebic liver abscess formation involves cyclooxygenase-2 expression in macrophages and trophozoites. | it has been demonstrated that expression of cyclooxygenase-2 (cox-2) isoform is induced by entamoeba histolytica in macrophages and polymorphonuclear cells during amoebic liver abscess (ala) formation in hamsters. trophozoites present in the lesion were also positive for cox-2 signal. however, no cross reactivity of the anti-cox-2 antibody with protein extract of cultivated trophozoites was found. to clarify if trophozoites are involved in pge(2) production during ala development, cox-2 expressi ... | 2006 | 16647055 |
| acetate kinase: not just a bacterial enzyme. | the bacterial enzymes acetate kinase (ak) and phosphotransacetylase (pta) form a key pathway for synthesis of the central metabolic intermediate acetyl coenzyme a (acetyl-coa) from acetate or for generation of atp from excess acetyl-coa. putative ak genes have now been identified in some eukaryotic microbes. in chlamydomonas reinhardtii and phytophthora species, ak forms a pathway with pta. ak has also been identified in non-yeast fungi but these fungi do not have pta. instead, ak forms a pathwa ... | 2006 | 16678422 |
| intestinal parasitic infections among school children of the dhahira region of oman. | to determine the prevalence of intestinal parasitic infections among primary school children in dhahira region of oman. | 2006 | 16680250 |
| unique posttranslational modifications of chitin-binding lectins of entamoeba invadens cyst walls. | entamoeba histolytica, which causes amebic dysentery and liver abscesses, is spread via chitin-walled cysts. the most abundant protein in the cyst wall of entamoeba invadens, a model for amebic encystation, is a lectin called eijacob1. eijacob1 has five tandemly arrayed, six-cys chitin-binding domains separated by low-complexity ser- and thr-rich spacers. e. histolytica also has numerous predicted jessie lectins and chitinases, which contain a single, n-terminal eight-cys chitin-binding domain. ... | 2006 | 16682461 |
| stress by heat shock induces massive down regulation of genes and allows differential allelic expression of the gal/galnac lectin in entamoeba histolytica. | gene expression analysis by microarray assay revealed that when exposed to stress, entamoeba histolytica exhibits a specific heat shock response, together with a dramatic overall reduction in gene transcription as well as differential allelic expression of key genes participating in virulence, such as the galactose/n-acetylgalactosamine (gal/galnac) lectin. | 2006 | 16682464 |
| the invasiveness of entamoeba histolytica - a continuing enigma. | | 1996 | 16696073 |
| an unsuspected cause of chronic colitis. | | 2006 | 16698754 |
| amebic liver abscess rupturing into the lesser omentum space. | a case of an amebic abscess localized in the lesser omentum is reported. there was no sign of a liver abscess in the imaging examination or the operative findings. however, it is likely that the amebic infection occurred after a liver abscess ruptured into the abdominal cavity. early diagnosis and therapy are required when an abscess of unknown origin borders the liver, given the possibility of amebic abscess. | 2006 | 16708304 |
| ehpak2, a novel p21-activated kinase, is required for collagen invasion and capping in entamoeba histolytica. | p21-activated kinases (paks) are a highly conserved family of enzymes that are activated by rho gtpases. all paks contain an n-terminal cdc42/rac interacting binding (crib) domain, which confers binding to these gtpases, and a c-terminal kinase domain. in addition, some paks such as cla4p, skm1p and pak2p contain an n-terminal pleckstrin homology (ph) domain and form a distinct group of pak proteins involved in cell morphology, cell-cycle and gene transcription. here, we describe a novel p21-act ... | 2006 | 16716419 |
| entamoeba histolytica-associated diarrheal illness is negatively associated with the growth of preschool children: evidence from a prospective study. | the enteric protozoa, cryptosporidium, giardia and entamoeba histolytica, cause diarrhea in children. we investigated the association of enteric protozoan-associated diarrheal illness with the nutritional status and growth of preschool children in dhaka, bangladesh. the subjects were 221 children aged 2-5 years who were followed prospectively for diarrheal illness for 3 years. the weight and height of the children were measured at entry and at 4-month intervals. cryptosporidium and e. histolytic ... | 2006 | 16730764 |
| antiamoebic activity of iridoids from morinda morindoides leaves. | an aqueous decoction (dried extract), an 80 % methanolic extract from morinda morindoides (rubiaceae) leaves, and five iridoids isolated from the 80 % methanolic extract were evaluated in vitro for their activity against entamoeba histolytica and their cytotoxicity. the aqueous decoction and the 80 % methanolic extract exhibited a promising antiamoebic activity with ic (50) values of 3.1 +/- 1.7 and 1.7 +/- 0.6 microg/ml, respectively. all tested iridoids displayed antiamoebic activity, the most ... | 2006 | 16732537 |
| transcriptional silencing of multiple genes in trophozoites of entamoeba histolytica. | in a previous work we described the transcriptional silencing of the amoebapore a (ap-a) gene (ehap-a) of entamoeba histolytica strain hm-1:imss. the silencing occurred following transfection with a plasmid containing a 5' upstream region (473 bp) of ehap-a that included a truncated segment (140 bp) of a short interspersed nuclear element (sine1). silencing remained in effect even after removal of the plasmid (clone g3). neither short interfering rna nor methylated dna were detected, but the chr ... | 2006 | 16733544 |
| increased expression of the major cysteine proteinases by stable episomal transfection underlines the important role of ehcp5 for the pathogenicity of entamoeba histolytica. | the protozoan entamoeba histolytica causes intestinal inflammation and liver abscess. cysteine proteinases (cps) have been proposed as important virulence factors for amoebiasis. to test the role of the various cps for amoeba induced pathology, the three major enzymes of the parasite, namely ehcp1, ehcp2 and ehcp5 accounting for about 90% of total proteinase activity, were overexpressed by stable episomal transfection. total cp activity of recombinant amoebae increased by three- to six-fold depe ... | 2006 | 16753229 |
| diagnostic methods for differentiation of entamoeba histolytica and entamoeba dispar in carriers: performance and clinical implications in a non-endemic setting. | unpreserved faecal samples, suspected to contain entamoeba histolytica/entamoeba dispar cysts or trophozoites on the basis of microscopic examination, and serum samples from 416 patients were collected in a prospective study to determine whether stool antigen assays and detection of antibodies in serum are reliable methods to distinguish between carriers of e. histolytica and e. dispar in comparison to the reference test: real-time pcr. in 283 patients (68%) dna of e. histolytica or e. dispar wa ... | 2006 | 16753339 |
| entamoeba histolytica cysteine proteases cleave the muc2 mucin in its c-terminal domain and dissolve the protective colonic mucus gel. | in order for the protozoan parasite entamoeba histolytica (e.h.) to cause invasive intestinal and extraintestinal infection, which leads to significant morbidity and mortality, it must disrupt the protective mucus layer by a previously unknown mechanism. we hypothesized that cysteine proteases secreted from the amoeba disrupt the mucin polymeric network, thereby overcoming the protective mucus barrier. the muc2 mucin is the major structural component of the colonic mucus gel. heavily o-glycosyla ... | 2006 | 16754877 |
| comparison of a stool antigen detection kit and pcr for diagnosis of entamoeba histolytica and entamoeba dispar infections in asymptomatic cyst passers in iran. | the present study was conducted to compare stool antigen detection with pcr for the diagnosis of entamoeba sp. infection in asymptomatic cyst passers from iran. entamoeba dispar and, in one case, e. moshkovskii were the entamoeba spp. found in the amebic cyst passers. there was a 100% correlation between the results from the techlab e. histolytica ii stool antigen kit and those from nested pcr. we concluded that e. dispar is much more common in asymptomatic cyst passers in iran and that antigen ... | 2006 | 16757634 |
| higher seroprevalence of entamoeba histolytica infection is associated with human immunodeficiency virus type 1 infection in taiwan. | we assessed the seroprevalence of entamoeba histolytica infection using indirect hemagglutination antibody (iha) assay among 667 hiv-infected persons (group a), 1,311 asymptomatic hiv-uninfected persons seeking anonymous hiv testing (group b), 616 hiv-uninfected controls with gastrointestinal symptoms (diarrhea and/or liver abscess) seeking medical care (group c), and 2,500 healthy controls undergoing health check-up (group d). an iha titer >or= 128 was detected in 7.1% of group a, 2.5% of group ... | 2006 | 16760513 |
| detection of entamoeba histolytica using polymerase chain reaction in pus samples from amebic liver abscess. | direct demonstration of entamoeba histolytica by conventional microscopy and in vitro culture in pus obtained from amebic liver abscess (ala) is often unsuccessful. we evaluated polymerase chain reaction (pcr) for detection of e. histolytica dna in such pus. | 2006 | 16763330 |
| amoebiasis cutis in hiv positive patient. | protozoan infections of the skin, particularly cutaneous amoebiasis, are rare in hiv-positive patients. we report a case of amoebiasis cutis in an hiv-positive truck driver with a history of frequent unprotected sexual exposures. he presented with multiple painful ulcers and sinuses with purulent discharge, necrotic slough and scarring in the perianal and gluteal region for the last 2 years. he was positive for hiv-1 and -2. cutaneous biopsy revealed numerous entamoeba histolytica in the trophoz ... | 2006 | 16766840 |
| cutaneous amebiasis in a child and review of the literature. | cutaneous amebiasis is a rare manifestation of infection with the parasite entamoeba histolytica. we describe this infestation in a 9-year-old mexican immigrant who presented with diarrhea and perianal ulcers. to our knowledge, this is only the tenth child with cutaneous amebiasis reported in the literature, and only the second from the united states. we review previous reports and emphasize the importance of a high index of clinical suspicion for early diagnosis and treatment to prevent potenti ... | 2006 | 16780468 |
| mucosal immunity to asymptomatic entamoeba histolytica and entamoeba dispar infection is associated with a peak intestinal anti-lectin immunoglobulin a antibody response. | we monitored 93 subjects cured of amebic liver abscess (ala) and 963 close associate controls in durban, south africa, and determined by enzyme-linked immunosorbent assay that the intestinal immunoglobulin a (iga) antibody response to the entamoeba histolytica galactose-inhibitable adherence lectin is most accurately represented by a complex pattern of transitory peaks. one or more intestinal anti-lectin iga antibody peaks occurred in 85.9% of ala subjects over 36 months compared to 41.6% of con ... | 2006 | 16790762 |
| studying cellular architecture in three dimensions with improved resolution: ta replicas revisited. | metal replicas have been used for surface analysis of biological structures with a variety of spatial resolutions. platinum (pt) has been the metal of choice because it provides very stable replicas and images of high contrast. some other metals, such as tantalum (ta) have been reported to provide better resolution on isolated macromolecular complexes and cellular structures. our goal is to study the gain in detail with ta and to evaluate if it provides enough detail and resolution to assist in ... | 2006 | 16798024 |
| the second cysteine protease inhibitor, ehicp2, has a different localization in trophozoites of entamoeba histolytica than ehicp1. | the genome of entamoeba histolytica contains two genes encoding inhibitors of cysteine proteases of the chagasin family. in contrast to that of ehicp1, the derived primary structure of the second inhibitor, ehicp2, possesses a typical n-terminal signal sequence. processed ehicp2 is as weakly related to amoebiasin-1 (27% identity) as to chagasin (identity 30%), indicating a different evolutionary origin of both amebic genes. by northern blots, we confirmed the expression of the ehicp2 gene, and i ... | 2006 | 16802137 |
| amoebicidal activity of milk, apo-lactoferrin, siga and lysozyme. | to identify amoebicidal components in human milk and the effect of iron on the amoebicidal activity. | 2006 | 16809402 |
| resistance of c57bl/6 mice to amoebiasis is mediated by nonhemopoietic cells but requires hemopoietic il-10 production. | resistance to intestinal amoebiasis is mouse strain dependent. c57bl/6 (b6) mice clear entamoeba histolytica within hours of challenge, whereas c3h and cba strains are susceptible to infection and disease. in this study, we show using bone marrow (bm) chimeric mice that mouse strain-dependent resistance is mediated by nonhemopoietic cells; specifically, b6 bm --> cba recipients remained susceptible as measured by amoeba score and culture, whereas cba bm --> b6 recipients remained resistant. inte ... | 2006 | 16818779 |
| progress in research on entamoeba histolytica pathogenesis. | entamoeba histolytica is a protozoan parasite of humans that causes 40,000-100,000 deaths annually. clinical amoebiasis results from the spread of the normally luminal parasite into the colon wall and beyond; the key development in understanding this complex multistage process has been the publication of the e. histolytica genome, from which has come an explosion in the use of microarrays to examine changes in gene expression that result from changes in growth conditions. the genome has also rev ... | 2006 | 16824782 |
| crohn's colitis perforation due to superimposed invasive amebic colitis: a case report. | the clinical and microscopic appearances of inflammatory bowel disease may be very similar to those of amebic colitis. the coexistence of invasive amebiasis with inflammatory bowel disease may have disastrous results. patients with inflammatory bowel disease have a greater prevalence of amebiasis, but this association is more significant for ulcerative colitis. there have been very few reports in the literature presenting the superimposition of amebiasis on crohn's disease. in this report, a rar ... | 2006 | 16830298 |
| cryptosporidiosis: prevalence, genotype analysis, and symptoms associated with infections in children in kenya. | cryptosporidium parasites are leading causes of enteric disease, especially in children. a prospective survey on the prevalence of cryptosporidiosis in children less than five years of age was undertaken at six microbiology laboratories in kenya on fecal samples submitted for routine parasite and ova investigations. analysis of 4,899 samples over a two-year study period showed an overall prevalence of cryptosporidiosis of 4% that was highest between november to february. investigations on the na ... | 2006 | 16837712 |
| suppression of nf-kappab activation by entamoeba histolytica in intestinal epithelial cells is mediated by heat shock protein 27. | little is known about the pathogenesis of entamoeba histolytica and how epithelial cells respond to the parasite. herein, we characterized the interactions between e. histolytica and colonic epithelial cells and the role macrophages play in modulating epithelial cell responses. the human colonic epithelial cell lines caco-2 and t84 were grown either as monoculture or co-cultured in transwell plates with differentiated human thp-1 macrophages for 24 h before stimulation with soluble amebic protei ... | 2006 | 16840786 |
| [frequency of human parasites in selected populations of silesian region]. | epidemiological evaluation of the most frequent human parasitoses in the group of children at age of seven years, in adult patients and in group of polish citizens coming back from tropics between 1999-2003 was performed. all examined people were silesian region inhabitants. the biggest average infection prevalence in children from all examined years concerned enterobius vermicularis, in adult--giardia intestinalis, while in group of population coming back from tropics--entamoeba histolytica-lik ... | 2005 | 16841687 |
| in vitro susceptibility of entamoeba histolytica and giardia lamblia to plants used in mexican traditional medicine for the treatment of gastrointestinal disorders. | in our search for new antiprotozoal chemotherapy, we collected a selection of 26 plants used in mexican traditional medicine for the treatment of gastrointestinal disorders. methanolic extracts of these species were screened for their antiprotozoal activity against entamoeba histolytica and giardia lamblia trophozoites using in vitro tests. among the tested extracts, the derivates of following species showed selectivity and significant antiprotozoal activity: chiranthodendron pentadactylon, anno ... | 2006 | 16846708 |
| very little intron gain in entamoeba histolytica genes laterally transferred from prokaryotes. | the evolution of spliceosomal introns remains intensely debated. we studied 96 entamoeba histolytica genes previously identified as having been laterally transferred from prokaryotes, which were presumably intronless at the time of transfer. ninety out of the 96 are also present in the reptile parasite entamoeba invadens, indicating lateral transfer before the species' divergence approximately 50 mya. we find only 2 introns, both shared with e. invadens. thus, no intron gains have occurred in ap ... | 2006 | 16847043 |
| distribution and differential diagnosis of entamoeba histolytica from entamoeba dispar by the pcr-rflp method in central iran. | entamoeba histolytica and entamoeba dispar are two morphologically indistinguishable human protozoan parasites that are genetically distinct species. the potential invasive pathogenic entamoeba histolytica and non-invasive parasite entamoeba dispar can be differentiated by molecular and other methods. we used polymerase chain reaction (pcr) to determine the ratio of the two species in a population in tehran and karaj in central iran. | 2003 | 16868369 |
| a bayesian approach to acute infectious diarrhea in adults. | acute infectious diarrhea is a yearly occurrence for most americans, and is associated with 1 million hospitalizations and about 6000 deaths in the united states annually. up to 80% of acute infectious diarrhea is caused by noroviruses, which produce a clinically mild illness with a predictable short course and good outcome that make laboratory testing and antimicrobial treatment unnecessary. most diarrhea-causing bacteria and protozoa can cause a clinical illness "like norovirus"; when they do ... | 2006 | 16880065 |
| treatment options for the eradication of intestinal protozoa. | pathogenic intestinal protozoa are responsible for clinically important infections in both the developed and the developing world. these organisms are responsible for both acute and chronic diarrhea, and entamoeba histolytica, which affects the colon, can spread to involve the liver. many of these pathogens, particularly the intracellular protozoa that predominantly affect the small intestine, produce their most devastating effects in patients with hiv/aids and other forms of immune deficiency. ... | 2006 | 16883348 |
| [selected parasitic and infectious diseases in persons returning from the tropics]. | due to dramatic development of modern means of transport, exotic countries located on distant continents are today within a few-hour reach by a jet. every year several million people travelled by air in business or as a tourists. this results in easy transmission of parasitic and infectious factors from continent to continent or from country to country. plasmodium vivax infection: diagnosis concerned 6 men who had returned from thailand, india, senegal and zaire. the parasitosis was detected as ... | 1999 | 16886451 |
| [candida and amoebiasis acquired in tropical and subtropical climatic zones]. | the objective of the study was the analysis of occurrence and number of candida in the alimentary tract of 209 patients with intestinal amoebiasis considering their state after the treatment. amoebiasis was diagnosed on the grounds of entamoeba histolytica present in the faeces, what has just recently been recognised equal to the presence of e. histolytica complex. in all patients serological investigations were performed using indirect haemagglutination (iha) test. the cases with relapses or wi ... | 1999 | 16886471 |
| cryptosporidium parvum and other instestinal parasites among diarrhoeal patients referred to ehnri in ethiopia. | the occurrence of intestinal parasites in patients referred to the parasitology laboratory of the ethiopian health and nutrition research institute (ehnri), was recorded for four consecutive years, 1998-2001. based on the stool appearance i.e. watery, loose, muciod and bloody, 442 diarrhoeal samples were particularly selected to allow diagnosis of intestinal parasites with emphasis on coccidian intestinal parasites. wet saline mounts and formol ether concentration method with logul's iodine were ... | 2004 | 16895037 |
| hepatogastric fistula in a pediatric patient. | hepatogastric fistula secondary to amebic liver abscess is extremely rare. only three pediatric cases have been reported in the english literature. percutaneous drainage of abscess along with parenteral metronidazole can prevent the need for extensive surgical intervention. timely intervention is usually followed by complete recovery. | 2006 | 16896810 |
| [worms that get on our nerves]. | | 2006 | 16910466 |
| eh klp5 is a divergent member of the kinesin 5 family that regulates genome content and microtubular assembly in entamoeba histolytica. | earlier studies have established two unusual features in the cell division cycle of entamoeba histolytica. first, microtubules form a radial assembly instead of a bipolar mitotic spindle, and second, the genome content of e. histolytica cells varied from 1x to 6x or more. in this study, eh klp5 was identified as a divergent member of the bimc kinesin family that is known to regulate formation and stabilization of the mitotic spindle in other eukaryotes. in contrast to earlier studies, we show he ... | 2007 | 16925786 |
| expression and function of a family of transmembrane kinases from the protozoan parasite entamoeba histolytica. | the signaling proteome of entamoeba histolytica is made of transmembrane kinases (tmks) that are rarely found in unicellular eukaryotes. there are 90 tmk genes reported for e. histolytica, and these have been grouped into nine distinct families based on motifs present on both extracellular and kinase domains. of these, the b1 family was chosen for further analysis. genomic sequencing revealed the presence of 28 members belonging to this family. genes corresponding to the majority of these were t ... | 2006 | 16926429 |
| entamoeba histolytica encephalitis diagnosed by pcr of cerebrospinal fluid. | extra-intestinal amebiasis is secondary to invasive intestinal infections and usually results in an amebic liver abscess. other organs, including lungs, brain, skin, spleen and kidneys, may be involved. diagnosis of the cerebral infection is of the utmost importance and is most often made by detection of the organism at the time of brain biopsy or at autopsy. we report the first case of entamoeba histolytica encephalitis diagnosed by pcr of the cerebrospinal fluid. the patient was treated succes ... | 2007 | 16930651 |
| microbicidal action of lactoferrin and lactoferricin and their synergistic effect with metronidazole in entamoeba histolytica. | lactoferrin (lf), in its iron-free form, has been shown to inhibit the growth of pathogenic microorganisms. in the light of new agents to control amoebiasis, the microbicidal activity of human and bovine lf and bovine lactoferricin (blfcin, fragment 4-14), and of each combined with metronidazole, the main drug used in amoebiasis, was evaluated in trophozoites of entamoeba histolytica. both lactoferrins and blfcin were able to kill amoebas in a concentration-dependent manner. this killing effect ... | 2006 | 16936803 |
| differential detection of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii by a single-round pcr assay. | a single-round pcr assay was developed for detection and differential diagnosis of the three entamoeba species found in humans, entamoeba moshkovskii, entamoeba histolytica, and entamoeba dispar, that are morphologically identical as both cysts and trophozoites. a conserved forward primer was derived from the middle of the small-subunit rrna gene, and reverse primers were designed from signature sequences specific to each of these three entamoeba species. pcr generates a 166-bp product with e. h ... | 2006 | 16954247 |
| new insights into the phylogeny of entamoeba species provided by analysis of four new small-subunit rrna genes. | sequences of small-subunit rrna genes have been obtained for four new isolates of entamoeba. phylogenetic analyses give new insights into the evolution of these organisms. a novel entamoeba from pigs in vietnam that produces uninucleate cysts proved to be unrelated to other uninucleated cyst-producing species. revival of the name entamoeba suis for this organism is proposed. instead of being related to entamoeba polecki, it shares a recent common ancestor with the non-encysting entamoeba gingiva ... | 2006 | 16957127 |
| comparative proteomic analysis of two entamoeba histolytica strains with different virulence phenotypes identifies peroxiredoxin as an important component of amoebic virulence. | entamoeba histolytica is a protozoan intestinal parasite that causes amoebic colitis and amoebic liver abscess. to identify virulence factors of e. histolytica, we first defined the phenotypes of two e. histolytica strains, hm-1:imss, the prototype virulent strain, and e. histolytica rahman, a strain that was reportedly less virulent than hm-1:imss. we found that compared with hm-1:imss, rahman has a defect in erythrophagocytosis and the ability to cause amoebic colitis in human colonic xenograf ... | 2006 | 16968225 |
| prevalence and species distribution of e. histolytica and e. dispar in the venda region, limpopo, south africa. | the prevalence and species distribution of entamoeba histolytica and e. dispar in the venda region were determined in stool samples collected from public hospitals and primary schools by elisa and a nested polymerase chain reaction (pcr). e. histolytica was detected in 37/197 (18.8%) and 1/47 (2.1%) samples, whereas 50/197 (25.3%) and 4/47 (8.5%) had e. dispar in the hospitals and schools, respectively. the age groups most infected were 0-2 (33%) years followed by 20-29 years (27%). e. histolyti ... | 2006 | 16968943 |