| The adhesion of oral bacteria to modified titanium surfaces: role of plasma proteins and electrostatic forces. | OBJECTIVES: Modifications of titanium (Ti) implant surfaces have a significant effect on early biofilm formation and the outcome of implant procedures. The aim of this study was to examine the role of plasma proteins and electrostatic forces in the adhesion mechanism of oral bacteria to modified Ti surfaces. MATERIALS AND METHODS: Ti discs with three different types of surface modifications, machined, acid-etched, and acid-etched and blasted, were examined for adhesion of oral bacteria: Streptoc ... | 2011 | 22150723 |
| hepatocytes produce tumor necrosis factor-α and interleukin-6 in response to porphyromonas gingivalis. | takano m, sugano n, mochizuki s, koshi rn, narukawa ts, sawamoto y, ito k. hepatocytes produce tumor necrosis factor-α and interleukin-6 in response to porphyromonas gingivalis. j periodont res; 2012; 47: 89-94. © 2011 john wiley & sons a/s background and objective: the liver plays a major role in clearing systemic bacterial infections. in addition, inflammatory cytokines produced in the liver play a critical role in systemic cytokine levels. the aim of this study was to investigate the product ... | 2011 | 21895661 |
| diode laser activated indocyanine green selectively kills bacteria. | commercially available photodynamic therapy for periodontal diseases utilizes methylene blue as a photosensitizer. here we propose a novel photosensitizer dye, indocyanine green (icg), because it can be readily activated by commercially available dental 810 nm diode lasers and has an established safety record as an intravascular agent in cardiac imaging and ophthalmologic photodynamic therapy. therefore, we aim to characterize icg uptake and killing of key periodontal pathogens to explore its po ... | 2011 | 21913603 |
| the effect of spiramycin on porphyromonas gingivalis and other "classic" periopathogens. | in clinical trials, spiramycin has shown additional benefit overscaling and root planing on pocket depth reduction, but its effect on periodontal microbiota was evaluated only by darkfield microscopy. therefore, this study was conducted to determine the effect of spiramycin administration on porphyromonas gingivalis and other periodontopathic bacteria using 16s rarn pcr technique. thirty two non-smoker adults with untreated periodontitis and pocket depth > or = 7 mm. were evaluated to participat ... | 2011 | 22010417 |
| clinical and microbiological evaluation, by real-time pcr, of non-surgical treatment of aggressive periodontitis associated with amoxicillin and metronidazole. | background: to evaluate the clinical and microbiological changes resulting from non-surgical periodontal treatment associated with amoxicillin and metronidazole, in individuals with aggressive periodontitis (agp). methods: 15 subjects with agp received non-surgical periodontal treatment and 45 days after completion of treatment, were treated with antibiotics. clinical data and samples of subgingival plaque were collected at baseline, 45 days after the non-surgical periodontal treatment and 1 mon ... | 2011 | 22060046 |
| anti-inflammatory effect of heme oxygenase-1 toward porphyromonas gingivalis lipopolysaccharide in macrophages exposed to gomisins a, g, and j. | periodontitis, a chronic inflammatory periodontal disease that develops from gingivitis, is caused by periodontal pathogenic bacteria such as porphyromonas gingivalis. recent studies have focused on the antioxidant, anti-human immunodeficiency virus, anticarcinogenic, and anti-inflammatory properties of gomisins. however, the anti-inflammatory activities of gomisin plants through heme oxygenase-1 (ho-1) signals remain poorly defined. we found that gomisins' anti-inflammatory activity occurs via ... | 2011 | 22145771 |
| [Antagonistic effects of Lactobacillus acidophilus and Bifidobacterium adolescents on periodontalpathogens in vitro]. | To evaluate the possible antagonistic effects of Lactobacillus acidophilus (La) and Bifidobacterium adolescents (Ba) on Porphyromonas gingivalis (Pg) and Fusobacteriurn nuclearum (Fn). | 2011 | 21909593 |
| Porphyromonas gingivalis sinks teeth into the oral microbiota and periodontal disease. | Periodontitis is linked to polymicrobial interactions and the presence of Porphyromonas gingivalis. In this issue of Cell Host & Microbe, Hajishengallis et al. (2011) demonstrate that P. gingivalis colonization in the oral cavity changes the composition of the oral commensal microbiota and accelerates microbiota-mediated bone-destructive periodontitis, indicating that this single, low-abundance species is a keystone in periodontal disease. | 2011 | 22100158 |
| Induction of interleukin (IL)-1ß, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans. | Trindade SC, Olczak T, Gomes-Filho IS, Moura-Costa LF, Cerqueira EMM, Galdino-Neto M, Alves H, Carvalho-Filho PC, Xavier MT, Meyer R. Induction of interleukin (IL)-1ß, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans. J Periodont Res 2012; 47: 27-32. © 2011 John Wiley & Sons A/S Background and Objective: Porphyromonas gingivalis, an anaerobic gram-negative bacterium, is associated with chronic periodontitis. This study was undertaken to evaluate the production of inte ... | 2011 | 21848614 |
| symptomatic and asymptomatic apical periodontitis associated with red complex bacteria: clinical and microbiological evaluation. | in this study, the association of red complex (rc) bacteria that include treponema denticola, tannerella forsythia and porphyromonas gingivalis with acute, exacerbated or chronic apical periodontitis was evaluated. seventy-one patients with periapical disease were evaluated by clinical examination and microbiological samples obtained from the root canals were analyzed by a polymerase chain reaction assay. twenty-one (29.6%) samples were positive for rc bacteria, with t. ... | 2011 | 22143381 |
| effect of a calcium hydroxide/chlorhexidine paste as intracanal dressing in human primary teeth with necrotic pulp against porphyromonas gingivalis and enterococcus faecalis. | international journal of paediatric dentistry 2012; 22: 116-124 background. intracanal medication is important for endodontic treatment success as it eliminates microorganisms that persist after biomechanical preparation. aim. to evaluate the effect of two intracanal medications against porphyromonas gingivalis and enterococcus faecalis in the root canals of human primary teeth with necrotic pulp with and without furcal/periapical lesion, using quantitative real-time polymerase chain reaction ... | 2011 | 21883559 |
| subgingival microbial consortia and the clinical features of periodontitis in adolescents. | this study aimed to investigate the association between microbial consortia and the clinical features of periodontitis using a multilevel modeling approach. a total of 958 sites in 87 adolescents with periodontitis (cases) and 73 controls were microbiologically sampled and clinically examined. associations between each of the clinical parameters clinical attachment, probing depth, supragingival plaque, calculus, bleeding on probing, and each of 18 bacterial species; and between the same clinical ... | 2011 | 22112031 |
| expression and possible immune-regulatory function of ghrelin in oral epithelium. | originally found in stomach mucosa, ghrelin is a peptide appetite hormone that has been implicated as an immuno-modulatory factor. ghrelin has also been found in salivary glands and saliva; however, its expression patterns and biological properties in the oral cavity remain unclear. therefore, we investigated the expression patterns of ghrelin in saliva, gingival crevicular fluid (gcf), and gingival tissue, as well as its in vitro effects on il-8 production by tnf-α or lps-stimulated oral epithe ... | 2011 | 21865591 |
| sublingual vaccination with fusion protein consisting of the functional domain of hemagglutinin a of porphyromonas gingivalis and escherichia coli maltose-binding protein elicits protective immunity in the oral cavity. | this study demonstrated that sublingual immunization with a fusion protein, 25k-haga-mbp, which consists of a 25-kda antigenic region of hemagglutinin a purified from porphyromonas gingivalis fused to maltose-binding protein (mbp) originating from escherichia coli as an adjuvant, elicited protective immune responses. immunization with 25k-haga-mbp induced high levels of antigen-specific serum igg and iga, as well as salivary iga. high level titers of serum igg and iga were also induced for almos ... | 2011 | 22066647 |
| porphyromonas gingivalis promotes monocyte migration by activating mmp-9. | zhou j, zhang j, chao j. porphyromonas gingivalis promotes monocyte migration by activating mmp-9. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01427.x. © 2011 john wiley & sons a/s background and objective: the migration of monocytes into the local environment is crucial for their maturation into macrophages or osteoclasts in the pathogenesis of periodontal disease. the objective of this study was to investigate the role and mechanisms mediated by porphyromonas gingivalis in promoting t ... | 2011 | 22035412 |
| tobacco smoke augments porphyromonas gingivalis-streptococcus gordonii biofilm formation. | smoking is responsible for the majority of periodontitis cases in the us and smokers are more susceptible than non-smokers to infection by the periodontal pathogen porphyromonas gingivalis. p. gingivalis colonization of the oral cavity is dependent upon its interaction with other plaque bacteria, including streptococcus gordonii. microarray analysis suggested that exposure of p. gingivalis to cigarette smoke extract (cse) increased the expression of the major fimbrial antigen (fima), but not the ... | 2011 | 22110637 |
| Associations of periodontal microorganisms with salivary proteins and MMP-8 in gingival crevicular fluid. | OBJECTIVE: We investigated in subjects with and without periodontitis, the levels of certain salivary proteins and matrix metalloproteinase-8 (MMP-8) in gingival crevicular fluid (GCF), in relation to the presence of specific periodontal pathogens. METHODS: Clinical parameters were recorded at baseline, in 1985 and in 2009 from 99 subjects; 56 with and 43 without periodontitis (mean age 59.2 ± SD 2.9). Saliva samples collected in 2009 were analysed for salivary albumin, total protein and immunog ... | 2011 | 22103335 |
| Porphyromonas gingivalis accelerates atherosclerosis in C57BL/6 mice fed a high-fat diet. | Objective: Porphyromonas gingivalis has been shown to accelerate atherosclerotic lesion development in atherosclerotic apo E-deficient mice. Here, we investigated whether repeated P. gingivalis injection affected the inflammatory and atherosclerotic responses of C57BL/6 mice fed a high-fat diet (HFD). Materials and methods: Eight-week-old C57BL/6 mice fed either HFD or a regular chow diet (RD) were inoculated intravenously with P. gingivalis or phosphate-buffered saline three times per week fo ... | 2011 | 22047042 |
| Detection of odoriferous subgingival and tongue microbiota in diabetic and nondiabetic patients with oral malodor using polymerase chain reaction. | Halitosis has been correlated with the concentration of volatile sulfur compounds (VSCs) produced in the oral cavity by metabolic activity of bacteria colonizing the periodontal pockets and the dorsum of the tongue. It has been assumed that there is a relationship between periodontal disease and diabetes mellitus. | 2011 | 21891897 |
| the bactericidal effects of an acidified sodium chlorite-containing oral moisturizing gel: a pilot study. | abstract the aim of this study was to examine the bactericidal effects and bactericidal time of an acidified sodium chlorite compound gel (asc-gel) on bacteria isolated from the peri-implant sulci of 10 patients who received implants 3-27 years previously, and the depth of each peri-implant sulcus was 5 mm or less. porphyromonas gingivalis (atcc33277) (p. gingivalis) was used as the control bacterium. five asc-gel preparations were created by adding 3.3%, 5.0%, 7.0%, 9.0%, and 11.0% citric acid ... | 2011 | 21905903 |
| porphyromonas gingivalis induces rankl in bone marrow stromal cells: involvement of the p38 mapk. | periodontitis is a bacterially-induced oral inflammatory disease that is characterised by tissue degradation and bone loss. porphyromonas gingivalis is a gram negative bacterial species highly associated with the pathogenesis of chronic periodontitis. receptor activator of nuclear factor-kb ligand (rankl) induces bone resorption whilst osteoprotegerin (opg) is a decoy receptor that blocks this process. cyclooxygenase-2 (cox-2) is an enzyme responsible for the production of prostaglandin (pge)(2, ... | 2011 | 21939752 |
| Asp- and Glu-specific novel dipeptidyl peptidase 11 of Porphyromonas gingivalis ensures utilization of proteinaceous energy sources. | Porphyromonas gingivalis and Porphyromonas endodontalis, asaccharolytic black-pigmented anaerobes, are predominant pathogens of human chronic and periapical periodontitis, respectively. They incorporate di- and tripeptides from the environment as carbon and energy sources. In the present study we cloned a novel dipeptidyl peptidase (DPP) gene of P. endodontalis ATCC 35406, designated as DPP11. The DPP11 gene encoded 717 amino acids with a molecular mass of 81,090 Da and was present as a 75-kDa f ... | 2011 | 21896480 |
| microbiologic findings 1 year after partial- and full-mouth scaling in the treatment of moderate chronic periodontitis. | to investigate the impact on microbiologic variables of full-mouth scaling (fms) and conventional scaling and root planing (csrp) after 12 months. | 2011 | 21909493 |
| animal models to study host-bacteria interactions involved in periodontitis. | animal models have distinct advantages because they can mimic cellular complexities that occur in humans in vivo and are often more accurate than in vitro studies that take place on plastic surfaces with limited numbers of cell types present. furthermore, cause and effect relationships can be established by applying inhibitors or activators or through the use of genetically modified animals. such gain or loss of function studies are often difficult to achieve in human clinical studies, particula ... | 2012 | 22142960 |
| electrochemically induced anatase inhibits bacterial colonization on titanium grade 2 and ti6al4v alloy for dental and orthopedic devices. | bacterial contamination of implanted devices is a common cause of their failure. the aim of the present study was to assess the capability of electrochemical procedures to: (a) promote the formation of anatase on the surface of commercially pure grade 2 ti and ti grade 5 (ti6al4v) alloy; (b) inhibit in vitro biofilm formation of staphylococcus aureus, staphylococcus epidermidis, streptococcus mutans and porphyromonas gingivalis and oral plaque in vivo, (c) preserve favorable response of osteobla ... | 2011 | 21862294 |
| Treatment of residual pockets with photodynamic therapy, diode laser, or deep scaling. A randomized, split-mouth controlled clinical trial. | The objective of this work was to compare the effects of antimicrobial photodynamic therapy (PDT), diode soft laser therapy (DSL), and thorough deep scaling and root planing (SRP) for treatment of residual pockets. Thirty-two subjects with a history of non-surgical treatment for chronic periodontitis were included. Residual pockets >4 mm and bleeding upon probing were debrided with an ultrasonic device and then subjected to either PDT, DSL, or SRP. Pocket probing depth (PPD), bleeding on probing ... | 2011 | 22105837 |
| Combined Effects of Hydrogen Sulphide and Lipopolysaccharide on Osteoclast Differentiation in Rats. | Background: Lipopolysaccharide (LPS) stimulates osteoclast differentiation through toll-like receptor (TLR) 2 and 4, and hydrogen sulphide (H(2)S) induces osteoclast differentiation. If H(2)S activates TLRs, H(2)S may enhance the effects of LPS on osteoclast differentiation. The purpose of the present study was to examine the combined effects of sodium hydrogen sulphide (NaHS, an H(2)S donor drug) and LPS on osteoclast differentiation and TLRs expression in rat periodontal tissue. Methods: Twent ... | 2011 | 21910595 |
| The comparative evaluation of xanthan gel with chlorhexidine (Chlosite) in smokers and non-smokers: A clinical and microbiological assessment. | Prevention of the periodontal disease progression is the primary goal of periodontal therapy. When conventional therapy is found inadequate to attain periodontal health in chronic periodontitis, local antimicrobial agents have been used as an adjunct with scaling and root planning (SRP) which has reproduced encouraging results. Hence, this study was undertaken to evaluate the new sustained released local drug Chlosite clinically and microbiologically in smokers and non-smokers. | 2011 | 22028508 |
| [cloning and expression of peptidylarginine deiminase of porphyromonas gingivalis]. | to clone the peptidylarginine deiminase(pad) gene of porphyromonas gingivalis(p.gingivalis) and to be expressed in e. coli under the best conditions. | 2011 | 22109358 |
| colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers. | gerodontology 2011; doi: 10.1111/j.1741-2358.2011.00506.x colonisation of the oral cavity by periodontopathic bacteria in complete denture wearers objective: the purpose of this study was to investigate colonisation by periodontopathic bacteria and the sites of colonisation in elderly upper and lower complete denture wearers. we also investigated the relationship between level of oral hygiene and colonisation by periodontopathic bacteria. materials and methods: forty edentulous and 37 dentate ... | 2011 | 21929616 |
| Osteoinductive and anti-inflammatory effect of royal jelly on periodontal ligament cells. | Royal jelly (RJ) has been reported to possess several physiological and pharmacological properties such as the ability to prevent osteoporosis in rats and anti-inflammatory effects. We hypothesized that RJ could have beneficial effects on the prevention or treatment of periodontal diseases, which are chronic inflammatory diseases caused by bacterial infection that result in resorption of the tooth-supporting bone. We assessed the effect of RJ on mineralization in mouse periodontal ligament cell ... | 2011 | 21878736 |
| understanding risk for periodontal disease. | an ability to identify individuals at risk for progressive periodontal disease would enable targeted prevention and treatment, thus reducing the economic burden on society. the importance of the interplay between microbial, genetic and environmental factors in risk assessment is recognized and the relative contributions of various risk factors has been determined. however, newer technologies will enable more accurate individual risk profiling in the future. | 2010 | 22046745 |
| Nicotine up-regulates IL-8 expression in human gingival epithelial cells following stimulation with IL-1ß or P. gingivalis lipopolysaccharide via nicotinic acetylcholine receptor signalling. | OBJECTIVE: Cigarette smoking is an important risk factor for periodontal disease. The aim of this study is to evaluate the effect of nicotine, a major component of cigarette smoke, on interleukin-8 (IL-8) production and cellular signalling via nicotinic acetylcholine receptors (nAChRs) in human gingival epithelial cells (HGECs). DESIGN: Messenger RNA (mRNA) expression of nAChR subunits in three different HGEC lines (epi 4, Tfx and E6E7) was assessed using reverse transcription-polymerase chain r ... | 2011 | 22119045 |
| [positive and negative regulation of transcription from hiv provirus]. | the rna genome of retroviruses including human immunodeficiency virus type 1 (hiv-1) will be converted into dna, called "propvirus". this proviral dna will be integrated into host cell genome and behave like host genes. since the step at which the viral rna genome is converted into dna will not allow any increase of viral genetic information because of the presence of rnaseh activity inherent to the reverse transcriptase and is responsible for the degradation of viral rna in forming the dna:rna ... | 2011 | 21972559 |
| immunization with porphyromonas gingivalis enolase induces autoimmunity to mammalian α-enolase and arthritis in dr4-ie-transgenic mice. | to examine the hypothesis that the subset of rheumatoid arthritis (ra) characterized by antibodies to citrullinated α-enolase is mediated by porphyromonas gingivalis enolase in the context of dr4 alleles. | 2011 | 21953289 |
| In vitro effects of novel toothpaste actives on components of oral malodour. | To evaluate the efficacy of a novel toothpaste containing zinc ions and o-cymen-5-ol to reduce volatile sulfur compounds (VSCs) in in vitro models and to elucidate the mode of action for any activity observed. | 2011 | 21762158 |
| Analysis of Porphyromonas gingivalis PG27 by deletion and intragenic suppressor mutation analyses. | PG27 is required for secretion of virulence factor gingipains, and has recently been proposed as LptO, which is involved in O-deacylation of lipopolysaccharide. In the present study, a predicted 14 anti-parallel ß-strand structure of PG27 was ascertained. Deletion study showed that the region from Asp382 to the C-terminal His391 of PG27 is dispensable for the function of PG27. Analysis of C-terminal deletion mutants revealed that the region in strand S14 (Asn369-Gly385) is important for activity ... | 2011 | 21896158 |
| clarithromycin suppresses the periodontal bacteria-accelerated abdominal aortic aneurysms in mice. | aoyama n, suzuki j-i, ogawa m, watanabe r, kobayashi n, hanatani t, yoshida a, ashigaki n, izumi y, isobe m. clarithromycin suppresses the periodontal bacteria-accelerated abdominal aortic aneurysms in mice. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01454.x. © 2011 john wiley & sons a/s background and objective: although clarithromycin (cam) has many biological functions, including regulation of mmps, little is known about its effect on abdominal aortic aneurysms. periodontopathic bac ... | 2011 | 22181324 |
| Randomized Controlled Trial Assessing Efficacy and Safety of Glycine Powder Air Polishing in Moderate to Deep Periodontal Pockets. | Background: Supragingivally applied glycine powder air polishing (Supra-GPAP) has been shown to remove biofilms in shallow periodontal pockets. This study assessed efficacy and safety of subgingivally applied glycine powder air polishing (Sub-GPAP) in moderate to deep periodontal pockets. Methods: Subjects with chronic periodontitis and intraoral Porphyromonas gingivalis and Tannerella forsythia having completed initial therapy were randomly assigned to receive Sub-GPAP in periodontal pockets wi ... | 2011 | 21861637 |
| in vitro activity of deferoxamine against porphyromonas gingivalis. | deferoxamine (dfo), an fda-approved iron chelator used for treatment of iron poisoning, affects bacteria as iron availability is intimately connected with growth and several virulence determinants. however, little is known about the effect on oral pathogens. in this study, the effect of dfo on porphyromonas gingivalis, a major periodontopathogen which has an essential growth requirement for hemin (fe(3+)-protoporphyrin ix), was evaluated. the viability of p. gingivalis w83 was not affected by 0. ... | 2011 | 22092680 |
| both the unique and repeat regions of the porphyromonas gingivalis hemagglutin a are involved in adhesion and invasion of host cells. | porphyromonas gingivalis is one of the major etiologic agents of adult periodontitis and has been associated with cardiovascular diseases. it expresses multiple hemagglutinins that are significant virulence factors and play an important role in bacterial attachment and invasion of host cells. the objective of this study was to determine the impact of p. gingivalis hemagglutinin a (haga) on the attachment to and invasion of human coronary artery endothelial cells (hcaec) and gingival epithelial c ... | 2011 | 22100486 |
| dna/protamine complex paste for an injectable dental material. | a dna/protamine complex powder was prepared by reaction between dna and protamine sulfate solution with stirring in order to develop a new injectable biomaterials for dental therapy. the powder of dna/protamine complex became paste by kneading the complex powder and distilled water. complex formation was confirmed by ft-ir measurement. the complex paste had a porous structure and its viscosity was approximately 280.1 pas. the paste could easily pass through a needle of 0.25 mm internal diameter. ... | 2011 | 21922330 |
| [Effect of CD-14 and toll like receptors on the expression of interleukin-6 induced by lipopolysaccharides of Porphyromonas endodontalis]. | To evaluate the effect of cluster of differentiation 14 (CD-14) and Toll like receptors (TLR) on the expression of interleukin-6 (IL-6) mRNA induced by Porphyromonas endodontalis (Pe) lipopolysaccharides (LPS). | 2011 | 22177355 |
| the capsule of porphyromonas gingivalis leads to a reduction in the host inflammatory response, evasion of phagocytosis, and increase in virulence. | periodontal disease is a chronic oral inflammatory disease that is triggered by bacteria such as porphyromonas gingivalis. p. gingivalis strains exhibit great heterogeneity, with some strains being encapsulated while others are nonencapsulated. although the encapsulated strains have been shown to be more virulent in a mouse abscess model, so far the role of the capsule in p. gingivalis interactions with host cells is not well understood and its role in virulence has not been defined. here, we in ... | 2011 | 21911459 |
| molecular detection of human periodontal pathogens in oral swab specimens from dogs in japan. | periodontal diseases are known to be major diseases in humans, and are also common in dogs. the purpose of the present study was to analyze the distribution of periodontitis-related bacterial species using oral swab specimens collected from 26 pet dogs. the distribution of an animal gingival organism porphyromonas gulae, in addition to 10 human periodontitis-related bacterial species, including porphyromonas gingivalis, treponema denticola, tannerella forsythia, capnocytophaga ochracea, capnocyt ... | 2011 | 21916371 |
| salivary biomarkers for predicting the progression of chronic periodontitis. | objective: predicting the progression of periodontitis would allow for targeted supportive periodontal therapy. the purpose of this study was to determine the usefulness of salivary biomarkers for predicting the progression of periodontitis. design: eighty-five chronic periodontitis patients were enrolled in an 18-month longitudinal study. amongst them, 57 experienced progression of periodontitis, indicated at the end of the 18 months by at least one site with >3mm loss of attachment compared wi ... | 2011 | 22030151 |
| Oral Bacterial DNA Differ in Their Ability to Induce Inflammatory Responses in Human Monocytic Cell Lines. | BACKGROUND: DNA of periodontal pathogens, Porphyromonas gingivalis (Pg-DNA) and Tannerella forsythia, stimulate cytokine production in human monocytic cells (THP-1) through Toll like receptor 9 (TLR-9) and nuclear factor kappa B signaling. Fusobacterium nucleatum is one of the most frequently isolated bacteria in periodontally-diseased tissues and reported to synergize with Porhyromonas gingivalis enhancing the pathogenicity. We investigated inflammatory mediator production in THP-1 cells challe ... | 2011 | 22166166 |
| colocalization of porphyromonas gingivalis with cd4+ t cells in periodontal disease. | porphyromonas gingivalis (p. gingivalis), an anaerobic, asaccharolytic gram-negative bacterium, is a causative agent in chronic periodontitis. it has many virulence factors that facilitate infection of the gingiva, but little is known about the local immune cells that respond to this bacterium. the aims of this study were to quantify p. gingivalis in gingival biopsies from patients with periodontitis using laser capture microdissection (lcm) plus qrt-pcr and to determine the phenotype of immune ... | 2011 | 22066676 |
| identification of the putative specific pathogenic genes of porphyromonas gingivalis with type ii fimbriae. | porphyromonas gingivalis, the key etiologic agent of periodontitis, can be classified into six types (i to v and ib) based on the fima genes that encode fima (a subunit of fimbriae). accumulated evidence indicates that p. gingivalis expressing type ii fimbriae (pg-ii) is the most frequent isolate from severe periodontitis cases and is more virulent than other types of p. gingivalis. however, during the pg-ii infection process, which specific virulence factors play the key role is still unclear. ... | 2012 | 22257441 |
| periodontitis, porphyromonas, and the pathogenesis of rheumatoid arthritis. | epidemiological data indicate a link between rheumatoid arthritis (ra) and periodontal disease (pd). in vitro and in vivo studies have sought to dissect potential mechanisms by which pd may contribute to initiation and progression of ra. however, these are both multifactorial, chronic diseases, and their complex etiologies and pathogenesis themselves remain incompletely understood. could there really be an etiological link or does this simply represent a statistical coincidence muddied by common ... | 2012 | 22274780 |
| microbial profile of patients with periodontitis compared with healthy subjects. | objective: to define and compare the microbiologic profile of subgingival plaque in german patients with periodontitis (including aggressive and advanced chronic periodontitis) and healthy subjects and to determine significant association between isolates and clinical status. aggregatibacter actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythia are major periodontal pathogens, though it is recognized that other species may also contribute to the pathogenesis of periodontal di ... | 2012 | 22257880 |
| full-mouth disinfection and systemic antimicrobial therapy in generalized aggressive periodontitis: a randomized, placebo-controlled trial. | aim: the present investigation aimed to analyse clinical and microbiological effects of systemic administration of metronidazole and amoxicillin combined with the one-stage-full-mouth-disinfection protocol (osfmd) in generalized aggressive periodontitis patients (g-agp). materials and methods: thirty-nine systemically healthy patients with g-agp were consecutively included. the test group (n = 19) received amoxicillin-metronidazole combination (500 mg of each, three times a day for 7 days) and t ... | 2012 | 22220822 |
| impact of supragingival therapy on subgingival microbial profile in smokers versus non-smokers with severe chronic periodontitis. | the aim of this study was to assess subgingival microbiological changes in smokers versus non-smokers presenting severe chronic periodontitis after supragingival periodontal therapy (st). | 2012 | 22232720 |
| the effects of bovine necrotic vulvo-vaginitis on reproductive and production performance of israeli 1st calf heifers. | bovine necrotic vulvovaginitis (bnvv) is a syndrome unique to israel characterized by necrotic lesion in the caudal vagina mainly in first calf heifers after calving, associated with porphyromonas levii. the objectives of this study were to analyze the impact of bnvv on reproductive performance, milk production and survival in the heard of first calf dairy heifers in affected farms, and to verify if the effects of bnvv are severity-dependent. for assessment of the severity level a scale of 4 deg ... | 2012 | 22225696 |
| [effects of lipopolysaccharides from various porphyromonas on the expression of cd14 and tlrs in mouse osteoblast]. | to observe the effect of lipopolysaccharides(lps) extracted from porphyromonas endodontalis(p.e) and porphyromonas gingivals(p.g) on the expression of cd14 and tlrs in osteoblast. | 2011 | 22241307 |
| oral microbial biofilm stimulation of epithelial cell responses. | oral bacterial biofilms trigger chronic inflammatory responses in the host that can result in the tissue destructive events of periodontitis. however, the characteristics of the capacity of specific host cell types to respond to these biofilms remain ill-defined. this report describes the use of a novel model of bacterial biofilms to stimulate oral epithelial cells and profile select cytokines and chemokines that contribute to the local inflammatory environment in the periodontium. monoinfection ... | 2012 | 22266273 |
| longitudinal assessment of pigtailed macaque lower genital tract microbiota by pyrosequencing reveals dissimilarity to the genital microbiota of healthy humans. | vaginal bacterial communities play an important role in human health and have been shown to influence hiv infection. pigtailed macaques (macaca nemestrina) are used as an animal model of hiv vaginal infection of women. since the bacterial microbiota could influence retrovirus infection of pigtailed macaques, the genital microbiota in 10 cycling macaques was determined by pyrosequencing. the microbiota of all macaques was poly-microbial with a median of 13 distinct genera. strikingly, the gen ... | 2012 | 22264029 |
| porphyromonas endodontalis in chronic periodontitis: a clinical and microbiological cross-sectional study. | although previous studies have shown the presence of porphyromonas endodontalis in chronic periodontitis associated with periapical lesions, the occurrence of this pathogen in diseased periodontal sites without periapical lesions has been poorly investigated. | 2012 | 22232719 |
| assessments of salivary antioxidant activity using electron spin resonance spectroscopy. | objective: in recent years, the function of saliva has been focused on evaluation of general status. the relationship between salivary antioxidant activity and periodontal disease progression is unclear. the aim of this study is to assess the relationship between periodontal disease and salivary antioxidant activity towards various reactive oxygen species (ros) using electron spin resonance (esr) technique. methods: we demonstrated that whole saliva derived rats or human subjects scavenged ros s ... | 2012 | 22261034 |
| periodontal pathogens affect the level of protease inhibitors in gingival crevicular fluid. | in periodontitis, an effective host-response is primarily related to neutrophils loaded with serine proteases, including elastase (ne) and protease 3 (pr3), the extracellular activity of which is tightly controlled by endogenous inhibitors. in vitro these inhibitors are degraded by gingipains, cysteine proteases produced by porphyromonas gingivalis. the purpose of this study was to determine the level of selected protease inhibitors in gingival crevicular fluid (gcf) in relation to periodontal i ... | 2012 | 22230465 |