| induction of type i interferons in response to bacterial stimuli in large yellow croaker larimichthys crocea. | in addition to the crucial roles in coordinating antiviral immune responses, type i interferons (ifns) also play a role in the host immunity against bacterial pathogens. our previous study identified two type i ifns from large yellow croaker larimichthys croaea(lc), lcifnd and lcifnh, and showed their strong induction by poly(i:c) and antiviral activities. in the present study, both lcifnd and lcifnh were found to be rapidly induced in head kidney and spleen by mixed bacteria of vibrio alginolyt ... | 2017 | 28119146 |
| [monitoring and research on pathogen spectrum in patients with acute diarrhea from sentinel hospital of zhejiang province during 2009 to 2014]. | objective: to explore pathogen spectrum constitution of acute diarrhea in outpatient and emergency of zhejiang province, and provide basis for treatment, prevention and control of the disease. methods: during january 2009 to december 2014, we selected seven sentinel hospitals in different regions of zhejiang, monitored and researched on pathogen spectrum in patients with acute diarrhea from outpatient and emergency. we recorded patients' personal basic information, the main symptoms and signs, a ... | 2016 | 28057113 |
| o-heterocyclic derivatives with antibacterial properties from marine bacterium bacillus subtilis associated with seaweed, sargassum myriocystum. | the brown seaweed, sargassum myriocystum associated with heterotrophic bacterium, bacillus subtilis mtcc 10407 (jf834075) exhibited broad-spectra of potent antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio vulnificus, and vibrio parahaemolyticus. b. subtilis mtcc 10407 was found to be positive for polyketide synthetase (pks) gene, and therefore, was considered to characterize secondary metabolites bearing polyketide backbone. using bioassay-guided fractionation, t ... | 2017 | 27624095 |
| molecular characteristics of lysozyme g in euryglossa orientalis; cdna cloning, phylogenic analysis, physicochemical properties and tissue gene expression. | lysozymes are the key molecules of innate immune system against bacterial infections. in the present study, we identified the molecular characteristics, physicochemical properties, antibacterial activity, evolutionary relationship and tissue expression pattern of g-type lysozyme in euryglossa orientalis (euor lysg). the full-length euor lysg cdna is composed of 588 nucleotides and an open reading frame encoding a protein with 195 amino acids with more than 65 % identity to g-type lysozyme of sol ... | 2016 | 27393386 |
| effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (venerupis philippinarum) and mussel (mytilus edulis). | the effect of acidic electrolyzed water (aew) on inactivating escherichia coli o104:h4, listeria monocytogenes, aeromonas hydrophila, vibrio parahaemolyticus and campylobacter jejuni in laboratory contaminated live clam (venerupis philippinarum) and mussel (mytilus edulis) was investigated. the initial levels of bacterial contamination were: in clam 4.9 to 5.7log10cfu/g, and in mussel 5.1 to 5.5log10cfu/g. two types of aew were used for treatment time intervals of 1 and 2h: strong (saew) with an ... | 2016 | 27208583 |
| antibacterial activity of litsea cubeba (lauraceae, may chang) and its effects on the biological response of common carp cyprinus carpio challenged with aeromonas hydrophila. | the aims of this study were to characterize the antibacterial activity and the chemotype of litsea cubeba leaf essential oil (eo) harvested in north vietnam and to investigate the biological effects induced by the leaf powder on growth, nonspecific immunity and survival of common carp (cyprinus carpio) challenged with aeromonas hydrophila. | 2016 | 27124660 |
| identification of polyvalent protective immunogens from outer membrane proteins in vibrio parahaemolyticus to protect fish against bacterial infection. | vaccination is one of the most effective and economic way to prevent infectious diseases in aquaculture. the development of effective vaccines, however, is still limited, especially for polyvalent vaccines, which are against multiple species. with this regard, identification of polyvalent protective immunogens, serving as polyvalent vaccines, became a key step in vaccine development. in the current study, 17 outer membrane proteins from vibrio parahaemolyticus were identified as immunogens. furt ... | 2016 | 27071519 |
| characterization and functional analysis of a tandem-repeat galectin-9 in large yellow croaker larimichthys crocea. | galectins are a family of endogenous lectins with β-galactosides affinity, playing significant roles in the innate immunity of vertebrates and invertebrates. in this report, a new galectin-9 cdna was identified and characterized in large yellow croaker larimichthys crocea (designated as lcgal-9). the complete cdna sequence of lcgal-9 was 1795 bp, with an open reading frame (orf) of 1032 bp encoding 343 amino acids. the putative lcgal-9 protein contained two carbohydrate recognition domains (crds ... | 2016 | 26997199 |
| characterization of blooming algae and bloom-associated changes in the water quality parameters of traditional pokkali cum prawn fields along the south west coast of india. | in tropical shrimp farms, especially in traditional pokkali shrimp ponds, poor water quality management can lead to serious threats like heavy algal blooms and frequent disease outbreaks. an investigation regarding the frequent disease outbreaks in selected pokkali shrimp pond adjoining the cochin backwaters has been conducted. water quality parameters were analyzed during the study period. results indicate a considerable fluctuation in the concentration of dissolved oxygen (do) in the study in ... | 2016 | 26850711 |
| a novel c-type lectin, nattectin-like protein, with a wide range of bacterial agglutination activity in large yellow croaker larimichthys crocea. | c-type lectins (ctls) are generally recognized as a superfamily of ca(2+)-dependent carbohydrate-binding proteins, which serve as pattern recognition receptors (prrs) in innate immunity of vertebrates. in this study, the molecular characterization and immune roles of a novel ctl from larimichthys crocea (designated as lcntc) were investigated. lcntc is a novel protein that shared 33%-49% homology with other teleosts ctls. the full-length cdna of lcntc was composed of 859 bp with a 465 bp open re ... | 2016 | 26828263 |
| a novel c-type lectin is involved in the innate immunity of macrobrachium nipponense. | c-type lectins (ctls) play important roles in invertebrate innate immunity by recognizing and eliminating pathogens. in the present study, a low-density lipoprotein receptor class a (ldla) domain-containing ctl was identified from the oriental river prawn macrobrachium nipponense, designated as mnctldcp1. the full-length cdna of mnctldcp1 was composed of 1462 bp, with a 999-bp orf encoding a 332-aa protein. an ldla and a single c-type lectin-like domain (ctld) were found. the mrna transcripts of ... | 2016 | 26804648 |
| inhibition of pseudomonas aeruginosa exsa dna-binding activity by n-hydroxybenzimidazoles. | the pseudomonas aeruginosa type iii secretion system (t3ss) is a primary virulence determinant and a potential target for antivirulence drugs. one candidate target is exsa, a member of the arac family of dna-binding proteins required for expression of the t3ss. a previous study identified small molecules based on an n-hydroxybenzimidazole scaffold that inhibit the dna-binding activity of several arac proteins, including exsa. in this study, we further characterized a panel of n-hydroxybenzimidaz ... | 2016 | 26574012 |
| identification of a novel clip domain serine proteinase (sp-csp) and its roles in innate immune system of mud crab scylla paramamosain. | clip domain serine proteinases and their homologs are involved in the innate immunity of invertebrates. to identify the frontline defense molecules against pathogenic infection, we isolated a novel clip domain serine proteinase (sp-csp) from the hemocytes of mud crab scylla paramamosain. the full-length 1362 bp sp-csp contains a 1155 bp open reading frame (orf) encoding 384 amino acids. multiple alignment analysis showed that the putative amino acid sequence of sp-csp has about 52% and 51% ident ... | 2015 | 26272638 |
| identification, characterization, and functional studies of a pelle gene in the chinese mitten crab, eriocheir sinensis. | the toll-like receptor/nf-κb signaling pathways play an important role in the innate immune system. in the present study, one pelle gene (named espelle) was identified for the first time from the chinese mitten crab eriocheir sinensis. the full-length cdna of espelle is 3797 bp with a 3156 bp-long open reading frame that encodes a 1051 amino acid protein. espelle protein contains a death domain at the n-terminal and a serine/threonine kinase domain at the c-terminal. a neighbor joining phylogene ... | 2015 | 26026692 |
| molecular characterization of a transmembrane c-type lectin receptor gene from ayu (plecoglossus altivelis) and its effect on the recognition of different bacteria by monocytes/macrophages. | c-type lectin receptors (ctlrs) play vital roles in immune responses as pattern-recognition receptors (prrs). in this study, we identified a novel c-type lectin receptor (pactlrc) gene from ayu, plecoglossus altivelis. predicted pactlrc is a single transmembrane receptor with a typical carbohydrate recognition domain (crd) at its c-terminus. sequence comparison and phylogenetic tree analysis showed that pactlrc was most closely related to atlantic salmon (salmo salar) clrc, but was significantly ... | 2015 | 26010409 |
| a novel integrin function in innate immunity from chinese mitten crab (eriocheir sinensis). | integrins belong to a superfamily of conserved α β heterodimeric cell surface receptors that have critical function in cell migration, differentiation, and survival. in this study, an integrin called esintegrin was identified from chinese mitten crab eriocheir sinensis. esintegrin cdna is 4415 bp long with a 2457 bp open reading frame that encodes an 818 amino acid protein. esintegrin contains a signal peptide, an integrin beta subunit (n-terminal portion of extracellular region) inb domain, an ... | 2015 | 26004499 |
| identification and molecular characterization of a peritrophin-like gene, involved in the antibacterial response in chinese mitten crab, eriocheir sinensis. | peritrophin was first isolated from insect peritrophic membrane (pm) and was thought to protect insects from invasion of microorganisms and to stimulate digestion of food. in this study, a peritrophin-like gene (espt) was obtained from eriocheir sinensis. the full length cdna of espt was 1232 bp, which contained 1005 bp orf encoding a protein of 334 amino acids, including a 22 amino acid signal peptide, and 3 conserved chitin binding type 2 domains (chtbd2) characterized by having a 6-cysteine m ... | 2015 | 25596425 |
| novel myeloid differentiation factor 88, esmyd88, exhibits estube-binding activity in chinese mitten crab eriocheir sinensis. | myeloid differentiation factor 88 (myd88) is a universal and essential adapter protein that participates in the activation of the toll-like receptor/interleukin-1 receptor-mediated signaling pathway. in the present study, a new myd88 gene (named esmyd88) was identified in the chinese mitten crab eriocheir sinensis. the cdna of esmyd88 was 2210 bp long with a 1416 bp open reading frame that encoded a protein with 472 amino acids. predicted esmyd88 protein had a death domain at the n-terminal and ... | 2014 | 25150191 |
| cloning and characterization of two different l-type lectin genes from the chinese mitten crab eriocheir sinensis. | l-type lectins contain a leguminous lectin domain and bind to high-mannose type oligosaccharides. in the secretory pathway, l-type lectins play crucial functions in the trafficking, sorting, and targeting of maturing glycoproteins. this study identified two novel l-type lectins, designated as esergic-53 and esvip36, from the chinese mitten crab eriocheir sinensis. the complete nucleotide sequence of ergic-53 cdna was 1955 bp, containing a 1506 bp open reading frame (orf) encoding a putative prot ... | 2014 | 24796868 |
| liposome-encapsulated cinnamaldehyde enhances zebrafish (danio rerio) immunity and survival when challenged with vibrio vulnificus and streptococcus agalactiae. | cinnamaldehyde, which is extracted from cinnamon, is a natural compound with activity against bacteria and a modulatory immune function. however, the antibacterial activity and immunostimulation of cinnamaldehyde in fish has not been well investigated due to the compound's poor water solubility. thus, liposome-encapsulated cinnamaldehyde (lec) was used to evaluate the effects of cinnamaldehyde on in vitro antibacterial activity against aquatic pathogens and in vivo immunity and protection parame ... | 2014 | 24632045 |
| molecular cloning and characterization of the lipopolysaccharide and β-1,3-glucan binding protein from oriental river prawn, macrobrachium nipponense. | the lipopolysaccharide and β-1,3-glucan binding protein (lgbp), one of the pattern recognition proteins, plays an important role in the innate immune response of invertebrates. a 1,506 bp full-length cdna of a lgbp gene was cloned and characterized from the oriental river prawn macrobrachium nipponense (named as mnlgbp). analysis of the nucleotide sequence revealed that the cdna clone has an open reading frame of 1,119 bp, encoding a protein of 372 amino acids including a 21-aa signal peptide. t ... | 2014 | 24584659 |
| function of two novel single-crd containing c-type lectins in innate immunity from eriocheir sinensis. | c-type lectin is one of the pattern-recognition proteins of the non-self-innate immune system in invertebrates. in this study, two novel c-type lectin cdnas (esctl1 and esctl2) of eriocheir sinensis were cloned and characterized. esctl1 has 169 amino acids, whereas esctl2 has 164 amino acids. these two lectins contain one carbohydrate-recognition domain. phylogenetic analysis showed that esctl1 and esctl2 were not clustered with other reported lectins from crabs. esctl1 and esctl2 were expressed ... | 2014 | 24561128 |
| antimicrobial function of the gapdh-related antimicrobial peptide in the skin of skipjack tuna, katsuwonus pelamis. | a 3.4 kda of antimicrobial peptide was purified from an acidified skin extract of skipjack tuna, katsuwonus pelamis, by preparative acid-urea-polyacrylamide gel electrophoresis and c18 reversed-phase hplc. a comparison of the n-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high sequence homology with the yfgap (yellowfin tuna glyceraldehyde-3-phosphate dehydrogenase-related antimicrobial peptide); thus, this peptide was identified as the skip ... | 2014 | 24412436 |
| exsa and lcrf recognize similar consensus binding sites, but differences in their oligomeric state influence interactions with promoter dna. | exsa activates type iii secretion system (t3ss) gene expression in pseudomonas aeruginosa and is a member of the arac family of transcriptional regulators. arac proteins contain two helix-turn-helix (hth) dna binding motifs. one helix from each hth motif inserts into the major groove of the dna to make base-specific contacts with the promoter region. the amino acids that comprise the hth motifs of exsa are nearly identical to those in lcrf/virf, the activators of t3ss gene expression in the path ... | 2013 | 24142246 |
| an ldla domain-containing c-type lectin is involved in the innate immunity of eriocheir sinensis. | c-type lectins (ctls) have crucial functions in recognizing and eliminating pathogens in innate immunity. this study identified a novel low-density lipoprotein receptor class a (ldla) domain-containing ctl, designated as esctldcp, from the chinese mitten crab eriocheir sinensis. the esctldcp cdna is 1258 bp long, with a 975 bp open reading frame that encodes a 324-amino acid protein. esctldcp contains a signal peptide, an ldla, and a single c-type lectin-like domain. esctldcp was only expressed ... | 2014 | 24140432 |
| effects of potential probiotic bacillus amyloliquefaciens [corrected] fptb16 on systemic and cutaneous mucosal immune responses and disease resistance of catla (catla catla). | effects of dietary administration of bacillus amyloliquefaciens fptb16 on systemic and mucosal immunity and disease resistance of catla (catla catla) against edwardsiella tarda infection were evaluated in the present study. the laboratory maintained b. amyloliquefaciens was used to study antagonistic activity against fish pathogenic bacteria by agar well diffusion assay. healthy catla were challenged by this bacterium for determination of its safety. for preparation of probiotic supplemented die ... | 2013 | 24012512 |
| survival of vibrio parahaemolyticus and aeromonas hydrophila in sea bream (sparus aurata) fillets packaged under enriched co(2) modified atmospheres. | the ability to survive of two pathogens (vibrio parahaemolyticus and aeromonas hydrophila) spread over sea bream fillets packaged under different modified atmospheres (maps) was studied at 0°c and 4°c under refrigerated storage. the atmospheres used were 60% co2/40% n2, 70% co2/30% n2 and 80% co2/20% n2 and a control batch packaged in air. head space gas analyses, microbial counts and confirming test of pathogenic bacteria were carried out during 16days. the results obtained showed that all the ... | 2013 | 23860282 |
| characterization of a gene encoding the outer membrane receptor for ferric enterobactin in aeromonas hydrophila atcc 7966(t). | aeromonas hydrophila atcc 7966(t) produces a catecholate siderophore amonabactin in response to iron starvation. in this study, we determined that this strain utilizes exogenously supplied enterobactin (ent) for growth under iron-limiting conditions. a homology search of the a. hydrophila atcc 7966(t) genomic sequence revealed the existence of a candidate gene encoding a protein homologous to vibrio parahaemolyticus irga that functions as the outer membrane receptor for ferric ent. sds-page show ... | 2013 | 23391908 |
| aeromonas hydrophila lateral flagellar gene transcriptional hierarchy. | aeromonas hydrophila ah-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. our results indicate that a. hydrophila lateral flagellar genes are transcribed at three levels (class i to iii genes) and share some similarities with, but have many important differences from, genes of vibrio parahaemolyticus. a. hydrophila lateral flagellum class i gene transcription is σ(70) dependent, which is consistent with the fact that latera ... | 2013 | 23335410 |
| antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. | dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. in this context, the validations of the traditional claim that the leaf extract of d. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. the bacterial biofilms were quantified by crystal violet staining. among the 17 bacterial pathogens screened ... | 2012 | 23115018 |
| purification and characterization of yfgap, a gapdh-related novel antimicrobial peptide, from the skin of yellowfin tuna, thunnus albacares. | a 3.4 kda of antimicrobial peptide was purified from an acidified skin extract of the yellowfin tuna, thunnus albacares, by preparative acid-urea-polyacrylamide gel electrophoresis and c(18) reversed-phase hplc. a comparison of the n-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high homology with the n-terminus of glyceraldehyde-3-phosphate dehydrogenase (gapdh); thus, this peptide was designated as the yellowfin tuna gapdh-related antimicro ... | 2012 | 22771964 |
| recombinant aeromonas hydrophila outer membrane protein 48 (omp48) induces a protective immune response against aeromonas hydrophila and edwardsiella tarda. | the gene coding for an outer membrane protein omp48 of aeromonas hydrophila isolated from an infected fish was cloned and sequenced. analysis of nucleotide sequence showed the omp48 gene to be an adhesin encoding a protein of 426 amino acids with high identity to the omp48 gene of aeromonas veronii, another fish pathogen. the gene belonged to the maltoporin group of porins and had high similarity to lamb porins of a. hydrophila, aeromonas salmonicida and vibrio parahaemolyticus. the expressed pu ... | 2012 | 22564558 |
| purification and characterization of an antimicrobial histone h1-like protein and its gene from the testes of olive flounder, paralichthys olivaceus. | an approximately 21 kda antimicrobial protein was purified from an acidified testis extract of olive flounder, paralichthys olivaceus, by ion-exchange and c(18) reversed-phase hplc. a comparison of the n-terminal amino acid sequence with those of other known antimicrobial polypeptides revealed high homology between this antimicrobial protein and other histone h1 molecules; thus, it was designated flounder histone h1-like protein (fh1lp). fh1lp showed potent antimicrobial activity against gram-po ... | 2012 | 22538352 |
| clinical and epidemiologic features of diarrheal disease due to aeromonas hydrophila and plesiomonas shigelloides infections compared with those due to vibrio cholerae non-o1 and vibrio parahaemolyticus in bangladesh. | using data from the international centre for diarrhoeal disease research, bangladesh (icddr,b) from 1996 to 2001, we compared the clinical features of diarrhea in patients with stool specimens yielding only a. hydrophila (189 patients; 1.4% of 13,970 patients screened) or p. shigelloides (253 patients) compared to patients with sole v. cholerae non-o1 infection (99 patients) or v. parahaemolyticus infection (126 patients). patients exhibited similar frequencies of fever (temperature >37.8°c), st ... | 2012 | 23762755 |
| antimicrobial activities of secondary metabolites and phylogenetic study of sponge endosymbiotic bacteria, bacillus sp. at agatti island, lakshadweep archipelago. | twenty-one species of sponges were recorded under the class of demospongiae and calcareous sponges of which 19 species were new to agatti reef. a total of 113 sponge endosymbiotic bacterial strains were isolated from twenty-one species of sponges and screened for antimicrobial activity. five bacterial strains of sponge endosymbiotic bacteria (seb) namely seb32, seb33, seb36, seb43 and seb51 showed antimicrobial activity against virulent marine fish pathogens such as vibrio alginolyticus, vibrio ... | 2016 | 28352539 |
| aeromonas salmonicida ati2 is an effector protein of the type three secretion system. | the bacterium aeromonas salmonicida, a fish pathogen, uses the type three secretion system (ttss) to inject effector proteins into host cells to promote the infection. the study of the genome of a. salmonicida has revealed the existence of ati2, a potential ttss effector protein. in the present study, a structure-function analysis of ati2 has been done to determine its role in the virulence of a. salmonicida. biochemical assays revealed that ati2 is secreted into the medium in a ttss-dependent m ... | 2013 | 23832001 |
| establishment and characterization of a skin epidermal cell line from mud loach, misgurnus anguillicaudatus, (mase) and its interaction with three bacterial pathogens. | a continuous skin epidermal cell line from mud loach (misgurnus anguillicaudatus) (mase cell line) was established with its application in bacteria infection demonstrated in this study. primary mase cell culture was initiated at 26 °c in dulbecco's modified eagle medium/f12 medium (1:1; ph7.2) supplemented with 20% fetal bovine serum (fbs). the primary mase cells in spindle morphology proliferated into a confluent monolayer within 2 weeks, and were continuously subcultured even in 10% fbs- dmem/ ... | 2016 | 27288257 |
| a severe case of aeromonas veronii biovar sobria travellers' diarrhoea characterized by vibrio parahaemolyticus co-isolation. | we report a severe case of travellers' diarrhoea in a patient returning from ecuador to italy with the concomitant presence of aeromonas veronii biovar sobria and vibrio parahaemolyticus in their faeces. based on diagnostic results, epidemiological information and the clinical outcome, we conclude that the real aetiological agent was a. veronii biovar sobria, while v. parahaemolyticus was only transient in the intestine of the patient. | 2013 | 23002063 |
| identification of type vi secretion systems in moritella viscosa. | the study describes the identification of type vi secretion systems (t6sss) in moritella viscosa, the aetiological agent of winter ulcer disease. despite the availability of commercial vaccines, m. viscosa causes significant financial losses in salmonid farming. the t6ss transports bacterial proteins from the cell into the environment or directly into host cells, and has been implicated with bacterial virulence. the aim of the study was to identify potential t6sss in m. viscosa and to determine ... | 2012 | 22425489 |
| modelling and mutational analysis of aspergillus nidulans urea, a member of the subfamily of urea/h⁺ transporters in fungi and plants. | we present the first account of the structure-function relationships of a protein of the subfamily of urea/h(+) membrane transporters of fungi and plants, using aspergillus nidulans urea as a study model. based on the crystal structures of the vibrio parahaemolyticus sodium/galactose symporter (vsglt) and of the nucleobase-cation-symport-1 benzylhydantoin transporter from microbacterium liquefaciens (mhp1), we constructed a three-dimensional model of urea which, combined with site-directed and c ... | 2014 | 24966243 |
| green synthesis of multifunctional silver and gold nanoparticles from the oriental herbal adaptogen: siberian ginseng. | pharmacologically active stem of the oriental herbal adaptogen, siberian ginseng, was employed for the ecofriendly synthesis of siberian ginseng silver nanoparticles (sg-agnps) and siberian ginseng gold nanoparticles (sg-aunps). first, for metabolic characterization of the sample, liquid chromatography-tandem mass spectrometry analysis (indicated the presence of eleutherosides a and e), total phenol content, and total reducing sugar were analyzed. second, the water extract of the sample mediated ... | 2016 | 27468232 |
| extracellular synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 and their biological applications. | the present study highlights the microbial synthesis of silver and gold nanoparticles by sporosarcina koreensis dc4 strain, in an efficient way. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry, which displayed maximum absorbance at 424nm and 531nm for silver and gold nanoparticles, respectively. the spherical shape of nanoparticles was characterized by field emission transmission electron microscopy. the energy dispersive x-ray spectroscopy and elemental ... | 2016 | 26992796 |
| a strategic approach for rapid synthesis of gold and silver nanoparticles by panax ginseng leaves. | the study highlights the synthesis of gold nanoparticles and silver nanoparticles by fresh leaves of panax ginseng, an herbal medicinal plant. the reduction of auric chloride and silver nitrate led to the formation of gold and silver nanoparticles within 3 and 45 min, at 80°c, respectively. the developed methodology was rapid, facile, ecofriendly and the utmost significant is quite economical, which did not require subsequent processing for reduction or stabilization of nanoparticles. the nanopa ... | 2016 | 26698271 |
| characterization and antimicrobial application of biosynthesized gold and silver nanoparticles by using microbacterium resistens. | various microorganisms were found to be cable of synthesizing gold and silver nanoparticles when gold and silver salts were supplied in the reaction system. the main objective of this study was to evaluate the extracellular synthesis of gold and silver nanoparticles by the type strain microbacterium resistens(t) [kacc14505]. the biosynthesized gold and silver nanoparticles were characterized by ultraviolet-visible spectroscopy (uv-vis), field emission transmission electron micrograph (fe-tem), e ... | 2016 | 26597594 |
| weissella oryzae dc6-facilitated green synthesis of silver nanoparticles and their antimicrobial potential. | nanoparticles and nanomaterials are at the prominent edge of the rapidly developing field of nanotechnology. recently, nanoparticle synthesis using biological resources has been found to be a new area with considerable prospects for development. biological systems are the masters of ambient condition chemistry and are able to synthesize nanoparticles by utilizing metal salts. in the perspective of the current initiative to develop green technologies for the synthesis of nanoparticles, microorgan ... | 2016 | 26212222 |
| biosynthesis, characterization, and antimicrobial applications of silver nanoparticles. | in the present study, the strain brevibacterium frigoritolerans dc2 was explored for the efficient and extracellular synthesis of silver nanoparticles. these biosynthesized silver nanoparticles were characterized by ultraviolet-visible spectrophotometry, which detected the formation of silver nanoparticles in the reaction mixture and showed a maximum absorbance at 420 nm. in addition, field emission transmission electron microscopy revealed the spherical shape of the nanoparticles. the dynamic l ... | 2015 | 25848272 |
| the development of a green approach for the biosynthesis of silver and gold nanoparticles by using panax ginseng root extract, and their biological applications. | the biosynthesis of nanoparticles has received attention because of the development of economic and environmentally friendly technology for the synthesis of nanoparticles. the study develops a convenient method for the green synthesis of silver and gold nanoparticles by utilizing fresh root extract of the four-year old panax ginseng plant, and evaluated the antimicrobial applications of silver nanoparticles against pathogenic microorganisms. p. ginseng is a well-known herbal medicinal plant, and ... | 2016 | 25771716 |
| kinneretia thg-sqi4 mediated biosynthesis of silver nanoparticles and its antimicrobial efficacy. | simple, facile, effective approach for the biosynthesis of silver nanoparticles using kinneretia species and its antimicrobial activity against human pathogens has been demonstrated in this study. kinneretia thg-sqi4 has been isolated from soil sample collected from shangqui, china. the synthesized nanoparticles were characterized by ultraviolet-visible spectrophotometry (uv-vis), field emission transmission electron microscopy (fe-tem), energy dispersive x-ray spectroscopy (edx), elemental mapp ... | 2017 | 28211298 |
| raw ready-to-eat seafood safety: microbiological quality of the various seafood species available in fishery, hyper and online markets. | microbiological quality of 206 raw ready-to-eat seafood samples was investigated according to species (gizzard shad, halibut, rockfish, tuna, oyster and squid) and distribution channels (fishery, hyper and online market). enumeration of aerobic plate count and total coliforms (tc) and pathogenic bacteria (bacillus cereus, staphylococcus aureus and vibrio parahaemolyticus) was performed, and level of microbiological quality was classified into four groups: satisfactory, acceptable, unsatisfactory ... | 2017 | 27747902 |
| biosynthesis of silver nanoparticles by novosphingobium sp. thg-c3 and their antimicrobial potential. | the present study described biosynthesis of silver nanoparticles (agnps) using a bacterial strain novosphingobium sp. thg-c3, isolated from soil, and their application in antibacterial activity. the maximum absorbance values of the synthesized agnps was measured at 406 nm in ultraviolet-visible spectrophotometry and were mostly spherical in shape with particle size in range of 8-25 nm by field emission transmission electron microscopy analysis. x-ray diffraction pattern corresponding to planes ( ... | 2017 | 27145847 |
| a rapid and simple real-time pcr assay for detecting foodborne pathogenic bacteria in human feces. | a rapid, simple method for detecting foodborne pathogenic bacteria in human feces is greatly needed. here, we examined the efficacy of a method that employs a combination of a commercial pcr master mix, which is insensitive to pcr inhibitors, and a dna extraction method which used sodium dodecyl benzene sulfonate (sdbs), and tween 20 to counteract the inhibitory effects of sdbs on the pcr assay. this method could detect the target genes (stx1 and stx2 of enterohemorrhagic escherichia coli, inva ... | 2016 | 26902211 |
| enteric bacteria isolated from diarrheal patients in korea in 2014. | the aim of this study was to characterize the pathogens responsible for causing diarrhea according to season, region of isolation, patient age, and sex as well as to provide useful data for the prevention of diarrheal disease. | 2015 | 26473090 |
| starch based biodegradable graft copolymer for the preparation of silver nanoparticles. | the synthesis and characterization of a novel biodegradable graft copolymer based on partially hydrolyzed polymethylacrylate (pma) grafted amylopectin (ap) was reported which was developed for the synthesis of silver nanoparticles from silver nitrate solution by facile green technique. the prepared graft copolymer was biodegradable which was shown by fungal growth. characterization of silver nanoparticles was carried out by uv-vis spectroscopy (417nm), hr-tem, saed and fesem analysis. the tem fi ... | 2015 | 26231330 |
| biogenic silver and gold nanoparticles synthesized using red ginseng root extract, and their applications. | in the present study, we report a green methodology for the synthesis of silver and gold nanoparticles, using the root extract of the herbal medicinal plant korean red ginseng. the silver and gold nanoparticles were synthesized within 1 h and 10 min respectively. the nanoparticles generated were not aggregated, and remained stable for a long time, which suggests the nature of nanoparticles. the phytochemicals and ginsenosides present in the root extract assist in reducing and stabilizing the syn ... | 2016 | 25706249 |
| microbiological quality of seasoned roasted laver and potential hazard control in a real processing line. | microbiological quality of laver, one of the edible seaweeds, has not been reported in a real processing line. laver or supplements were collected from six manufacturers (a to f) to assess potential microbiological hazards and the critical control points in commercial processing lines. aerobic plate counts (apc), coliform counts, bacillus cereus, staphylococcus aureus, and vibrio parahaemolyticus were enumerated, and the presence of b. cereus, listeria monocytogenes, salmonella, s. aureus, and v ... | 2014 | 25474052 |
| enhancement of the immune response and protection against vibrio parahaemolyticus by indigenous probiotic bacillus strains in mud crab (scylla paramamosain). | in a previous study, bacterial communities of the intestine in three populations of crabs (wild crabs, pond-raised healthy crabs and diseased crabs) were probed by culture-independent methods. in this study, we examined the intestinal communities of the crabs by bacterial cultivation with a variety of media. a total of 135 bacterial strains were isolated from three populations of mud crabs. the strains were screened for antagonistic activity against vibrio parahaemolyticus using an agar spot ass ... | 2014 | 25193866 |
| a multiplex pcr assay for simultaneous detection of escherichia coli o157:h7, bacillus cereus, vibrio parahaemolyticus, salmonella spp., listeria monocytogenes, and staphylococcus aureus in korean ready-to-eat food. | a multiplex polymerase chain reaction (pcr) assay was developed for simultaneous detection of escherichia coli o157:h7, bacillus cereus, vibrio parahaemolyticus, salmonella spp., listeria monocytogenes, and staphylococcus aureus in various korean ready-to-eat foods. the six specific primer pairs for multiplex pcr were selected based on the o157 antigen (rfbe) gene of e. coli o157:h7, the dna gyrase subunit b (gyrb) gene of b. cereus, the toxin regulatory protein (toxr) gene of v. parahaemolyticu ... | 2014 | 24796416 |
| multiplex pcr (polymerase chain reaction) assay for detection of e. coli o157:h7, salmonella sp., vibrio cholerae and vibrio parahaemolyticus in spiked shrimps (penaeus monodon). | the coastal aquaculture mainly shrimps constitute major export sector in bangladesh and is increasingly shaped by international trade conditions and by national responses to those stringent quality and safety standards. pcr based validated methods for detection of major bacterial pathogens in shrimp might be very useful tool for ensuring quality and safety standards of exportable shrimps. the objective of this study was to evaluate overall performance (sensitivity and specificity) of the multipl ... | 2013 | 24498789 |
| microbiological quality and risk factors related to sandwiches served in bakeries, cafés, and sandwich bars in south korea. | the objective of this study was to investigate the microbiological quality of sandwiches produced on site and served in bakeries, cafés, and sandwich bars in south korea and to determine the major risk factors affecting the sanitation level in each store (n = 1,120). the microbiological quality of the sandwiches was analyzed, and the sanitation level of each store was evaluated as satisfactory or unsatisfactory based on sanitation guidelines. total coliforms were detected in 906 samples (80.9%), ... | 2013 | 23433370 |
| probiotics in the intestinal tract of juvenile whiteleg shrimp litopenaeus vannamei: modulation of the bacterial community. | molecular analysis of the 16s rdna of the intestinal microbiota of whiteleg shrimp litopenaeus vannamei was examined to investigate the effect of a bacillus mix (bacillus endophyticus yc3-b, bacillus endophyticus c2-2, bacillus tequilensisyc5-2) and the commercial probiotic (alibio(®)) on intestinal bacterial communities and resistance to vibrio infection. pcr and single strain conformation polymorphism (sscp) analyses were then performed on dna extracted directly from guts. injection of shrimp ... | 2013 | 23161451 |
| a preliminary study on the antibacterial mechanism of tegillarca granosa hemoglobin by derived peptides and peroxidase activity. | the blood clam, tegillarca granosa, is one of the few bivalve molluscs containing hemoglobin (hb). in the present study, we purified two types of t. granosa hemoglobin, tg-hbi and tg-hbii, using size exclusion chromatography and measured their antibacterial and peroxidase activities. we also tested antibacterial activities of peptides prepared by trypsin digestion of purified tg-hb and reversed-phase high-performance liquid chromatography purification. purified tg-hbi and tg-hbii showed antibact ... | 2016 | 26876330 |
| biological therapeutics of pongamia pinnata coated zinc oxide nanoparticles against clinically important pathogenic bacteria, fungi and mcf-7 breast cancer cells. | the overuse of antimicrobics and drugs has led to the development of resistance in a number of pathogens and parasites, which leads to great concerns for human health and the environment. furthermore, breast cancer is the second most common cause of cancer death in women. mcf-7 is a widely used epithelial cancer cell line, derived from breast adenocarcinoma for in vitro breast cancer studies, since the cell line has retained several ideal characteristics particular to the mammary epithelium. in ... | 2017 | 28115262 |
| recombinant expression of a modified shrimp anti-lipopolysaccharide factor gene in pichia pastoris gs115 and its characteristic analysis. | anti-lipopolysaccharide factors (alfs) with a lps-binding domain (lbd) are considered to have broad spectrum antimicrobial activities and certain antiviral properties in crustaceans. fcalf2 was one isoform of alfs isolated from the chinese shrimp fenneropenaeus chinensis. our previous study showed that a modified lbd domain (named lbdv) of fcalf2 exhibited a highly enhanced antimicrobial activity. in the present study, a modified fcalf2 gene (mfcalf2), in which the lbd was substituted by lbdv, w ... | 2016 | 27517939 |
| gfp tagged vibrio parahaemolyticus dahv2 infection and the protective effects of the probiotic bacillus licheniformis dahb1 on the growth, immune and antioxidant responses in pangasius hypophthalmus. | in this study, the pathogenicity of gfp tagged vibrio parahaemolyticus dahv2 and the protective effect of the probiotic strain, bacillus licheniformis dahb1 was studied on the asian catfish, pangasius hypophthalmus. the experiment was carried out for 24 days with three groups and one group served as the control (without treatment). in the first group, p. hypophthalmus was orally infected with 1 ml of gfp tagged v. parahaemolyticus dahv2 at two different doses (10(5) and 10(7) cfu ml(-1)). in the ... | 2016 | 26952171 |
| biosynthesis of silver nanoparticles using a probiotic bacillus licheniformis dahb1 and their antibiofilm activity and toxicity effects in ceriodaphnia cornuta. | in the present study, we synthesized and characterized a probiotic bacillus licheniformis cell free extract (blcfe) coated silver nanoparticles (blcfe-agnps). these blcfe-agnps were characterized by uv-visible spectrophotometer, xrd, edx, ftir, tem and afm. a strong surface plasmon resonance centered at 422 nm in uv-visible spectrum indicates the formation of agnps. the xrd spectrum of silver nanoparticles exhibited 2θ values corresponding to the silver nanocrystal. tem and afm showed the agnps ... | 2016 | 26802520 |
| quorum-quenching activity of the ahl-lactonase from bacillus licheniformis dahb1 inhibits vibrio biofilm formation in vitro and reduces shrimp intestinal colonisation and mortality. | vibrio parahaemolyticus is a significant cause of gastroenteritis resulting from the consumption of undercooked sea foods and often cause significant infections in shrimp aquaculture. vibrio virulence is associated with biofilm formation and is regulated by n-acylated homoserine lactone (ahl)-mediated quorum sensing. in an attempt to reduce vibrio colonisation of shrimps and mortality, we screened native intestinal bacilli from indian white shrimps (fenneropenaeus indicus) for an isolate which s ... | 2014 | 25060960 |
| isolation and characterisation of bacillus spp. antagonistic to vibrio parahaemolyticus for use as probiotics in aquaculture. | acute gastroenteritis caused by pathogenic vibrio parahaemolyticus is one of the major factors affecting the development of aquaculture and the safety of seafood. using the antagonism of probiotics against pathogens is an alternative strategy to antibiotics and a common trend to control food-borne pathogenic bacteria. in this study, a total of 249 isolates were isolated from four types of seafood (litopenaeus vannamei, oratosquilla oratoria, mactra veneriformis and portunus trituberculatus) and ... | 2015 | 25737203 |
| control of biofilm forming clinically important bacteria by green synthesized zno nanoparticles and its ecotoxicity on ceriodaphnia cornuta. | zinc oxide nanoparticles were synthesized using the aqueous leaf extracts of plectranthus barbatus (plb-zno nps) and characterized by uv-visible spectroscopy, xrd, ftir, sem and eds. uv-visible spectra recorded the absorbance peak of plb-zno nps at 343 nm. sem analyses showed the spherical shape of plb-zno nps with the particle size between 30 and 60 nm. plb-zno nps exhibited antibacterial and antibiofilm activity against gram positive bacillus subtilis at all tested concentrations. in contrast, ... | 2017 | 28330748 |
| a specific antimicrobial protein cap-1 from pseudomonas sp. isolated from the jellyfish cyanea capillata. | a bacterium strain, designated as cmf-2, was isolated from the jellyfish cyanea capillata and its culture supernatant exhibited a significant antimicrobial activity. the strain cmf-2 was identified as pseudomonas sp. based on the morphological, biochemical and physiological characteristics as well as 16s rrna sequence analysis. in this study, an antimicrobial protein, named as cap-1, was isolated from the culture of cmf-2 through ammonium sulfate precipitation and gel filtration chromatography. ... | 2016 | 26529191 |
| identification and functional analysis of a hemolin like protein from litopenaeus vannamei. | hemolin is a specific immune protein belonging to immunoglobulin superfamily and firstly identified in insects. growing evidences suggest that hemolin can be activated by bacterial and viral infections and may play an important role in antimicrobial immunity. in this paper, we firstly identified a hemolin-like protein from litopenaeus vannamei (lvhemolin). sequence analysis showed that lvhemolin shares high similarity with insect hemolins and is mainly composed of seven immunoglobulin (ig) domai ... | 2015 | 25527138 |
| enzymatic elucidation of haemocyanin from kuruma shrimp marsupenaeus japonicus and its molecular recognition mechanism towards pathogens. | haemocyanin is an important non-specific immune protein present in the hemolymph of invertebrates, which have the ability to recognize the microbial pathogens and trigger the innate immune system. in this study, we isolated and purified the haemocyanin using gel filtration chromatography and investigated its microbial recognition mechanism against the invading pathogens. kuruma shrimp marsupenaeus japonicus haemocyanin showed the single band with a molecular weight of 76 kda on sds-page and its ... | 2015 | 25204648 |
| ptlgbp, a pattern recognition receptor in portunus trituberculatus involved in the immune response against different challenges. | lipopolysaccharide and b-1,3-glucan binding protein (lgbp) is a pattern recognition receptor that can recognize and bind lps and b-1,3-glucan. lgbp has crucial roles in innate immune defense against gram-negative bacteria and fungi. in this study, lgbp functions in portunus trituberculatus innate immunity were analyzed. first, the mrna expression of ptlgbp in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (pamps) stimulations were examined using ... | 2014 | 25194733 |
| identification of a c-type lectin with antiviral and antibacterial activity from pacific white shrimp litopenaeus vannamei. | c-type lectins (ctls) play crucial roles in innate immune responses in invertebrates by recognizing and eliminating microinvaders. in this study, a ctl from pacific white shrimp litopenaeus vannamei (lvctl3) was identified. lvctl3 contains a single c-type lectin-like domain (ctld), which shows similarities to those of other shrimp ctls and has a mutated 'epd' motif in ca(2+)-binding site 2. lvctl3 mrna can be detected in all tested tissues and expression of lvctl3 in gills was up-regulated after ... | 2014 | 24792214 |
| biological characteristics and probiotic effect of leuconostoc lactis strain isolated from the intestine of black porgy fish. | a strain of lactic acid bacteria, leuconostoc lactis, was isolated from the intestinal tract of black porgy, sparus macrocephalus, and identified by conventional biochemical characteristics and 16s rdna gene sequence analysis. the isolated strain had the ability of bile tolerance and resistance to low ph, and survived well in the trypsinase and pepsin solution. but the highly concentrated dose of trypsinase and pepsin affect the viability of the isolated strain. the isolate was resistant to seve ... | 2013 | 24516418 |
| antimicrobial function of shβap, a novel hemoglobin β chain-related antimicrobial peptide, isolated from the liver of skipjack tuna, katsuwonus pelamis. | a 2.3 kda of antimicrobial peptide was purified from an acidified liver extract of skipjack tuna, katsuwonus pelamis, by preparative acid-urea-polyacrylamide gel electrophoresis and c18 reversed-phase hplc. a comparison of the amino acid sequence of the purified peptide with those of other known polypeptides revealed high homology with the c-terminus of hemoglobin β-chain; thus, this peptide was designated as the skipjack hemoglobin β chain-related antimicrobial peptide (shβap). shβap showed pot ... | 2014 | 24495783 |
| cgmolluscidin, a novel dibasic residue repeat rich antimicrobial peptide, purified from the gill of the pacific oyster, crassostrea gigas. | a 5.5 kda antimicrobial peptide consisting of 55 amino acids, cgmolluscidin, was purified from the acidified gill extract of the pacific oyster, crassostrea gigas, by ion-exchange and c18 reversed-phase high performance liquid chromatography. by comparing the n-terminal amino acid sequences and the molecular weight of this peptide with those of other known antimicrobial peptides, it has been revealed that this peptide had no homology with any known peptides. cgmolluscidin showed potent antimicro ... | 2013 | 23711469 |
| expression of allograft inflammatory factor-1 (aif-1) in response to bacterial challenge and tissue injury in the pearl oyster, pinctada martensii. | allograft inflammatory factor-1 (aif-1), an interferon (ifn)-γ-inducible calcium-binding cytokine, is associated with the inflammatory response and defense. we cloned and analyzed the expression pattern of the aif-1 gene of the pearl oyster pinctada martensii, hereafter designated pmaif-1. the full-length pmaif-1 cdna is 946 bp in length and consists of a 5'-untranslated region (utr) of 120 bp, a 3'-utr of 376 bp, and an open reading frame (orf) of 450 bp encoding a polypeptide of 149 amino acid ... | 2013 | 23178259 |
| selection and identification of non-pathogenic bacteria isolated from fermented pickles with antagonistic properties against two shrimp pathogens. | in this study, potential probiotic strains were isolated from fermented pickles based on antagonistic activity against two shrimp pathogens (vibrio harveyi and vibrio parahaemolyticus). two strains l10 and g1 were identified by biochemical tests, followed by16s ribosomal rna gene sequence analysis as bacillus subtilis, and characterized by pcr amplification of repetitive bacterial dna elements (rep-pcr). subsequently, b. subtilis l10 and g1 strains were tested for antibacterial activity under di ... | 2012 | 22491136 |
| purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties. | the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ... | 2017 | 28110033 |
| green synthesis of silver nanoparticles by bacillus methylotrophicus, and their antimicrobial activity. | the synthesis of silver nanoparticles (agnps) by microorganisms is an area attracting growing interest in nanobiotechnology, due to the applications of these nanoparticles in various products including cosmetics and biosensors, and in the biomedical, clinical, and bioimaging fields as well. various microorganisms have been found to be able to synthesize agnps when silver salts are supplied in the reaction system. the main objectives of this study were to evaluate the efficiency of synthesis of a ... | 2016 | 25749281 |
| case-control study of diarrheal disease etiology in individuals over 5 years in southwest china. | acute diarrhea is one of the major public health problems worldwide. most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in china. | 2016 | 27891182 |
| impact of co-infections with enteric pathogens on children suffering from acute diarrhea in southwest china. | acute diarrhea is a global health problem, resulting in high morbidity and mortality in children. it has been suggested that enteric pathogen co-infections play an important role in gastroenteritis, but most research efforts have only focused on a small range of species belonging to a few pathogen groups. this study aimed to assess the impact of co-infections with a broad range of enteric pathogens on children aged below five years who suffer from acute diarrhea in southwest china. | 2016 | 27349521 |
| a duplex quantitative real-time pcr assay for the detection of haplosporidium and perkinsus species in shellfish. | a duplex quantitative real-time polymerase chain reaction (dq-pcr) assay was optimized to simultaneously detect haplosporidium spp. and perkinsus spp. of shellfish in one reaction. two sets of specific oligonucleotide primers for haplosporidium spp. and perkinsus spp., along with two hydrolysis probes specific for each parasite group, were used in the assay. the dq-pcr results were detected and analyzed using the light cycler 2.0 software system. the dq-pcr identified and differentiated the two ... | 2013 | 23371501 |
| immunological function of familial mediterranean fever disease protein pyrin. | pyrin, encoded by mefv gene, is conserved in humans and mice. mutations in the mefv gene are associated with the human autoinflammatory disease familial mediterranean fever (fmf). pyrin can interact with the inflammasome adaptor asc and induce inflammatory caspase-1 activation in monocytic cells, but the physiological function of pyrin has been unknown for many years. here we summarize previous studies of pyrin function under the context of fmf and immunity, and discuss a recent study demonstrat ... | 2014 | 25307949 |
| innate immune sensing of bacterial modifications of rho gtpases by the pyrin inflammasome. | cytosolic inflammasome complexes mediated by a pattern recognition receptor (prr) defend against pathogen infection by activating caspase 1. pyrin, a candidate prr, can bind to the inflammasome adaptor asc to form a caspase 1-activating complex. mutations in the pyrin-encoding gene, mefv, cause a human autoinflammatory disease known as familial mediterranean fever. despite important roles in immunity and disease, the physiological function of pyrin remains unknown. here we show that pyrin mediat ... | 2014 | 24919149 |
| a probe-free four-tube real-time pcr assay for simultaneous detection of twelve enteric viruses and bacteria. | we aim to develop a multiplex real-time pcr assay to detect the most common pathogens causing community outbreaks of diarrhea. | 2015 | 26342434 |
| an improved multiplex real-time sybr green pcr assay for analysis of 24 target genes from 16 bacterial species in fecal dna samples from patients with foodborne illnesses. | here, we developed a new version of our original screening system (rapid foodborne bacterial screening 24; rfbs24), which can simultaneously detect 24 genes of foodborne pathogens in fecal dna samples. this new version (rfbs24 ver. 5) detected all known stx2 subtypes, enterotoxigenic escherichia coli (sth genotype), and vibrio parahaemolyticus (trh2), which were not detected by the original rfbs24 assay. the detection limits of rfbs24 ver. 5 were approximately 5.6 × 10(-2)-5.6 × 10(-5) (ng dna)/ ... | 2016 | 26166499 |
| a modified molecular beacons-based multiplex real-time pcr assay for simultaneous detection of eight foodborne pathogens in a single reaction and its application. | foodborne disease outbreaks are often caused by one of the major pathogens. early identification of the causal pathogen is crucial for disease control and prevention. we describe a real-time polymerase chain reaction (rtpcr) assay that can identify, in a single reaction, up to eight common foodborne bacterial pathogens, including salmonella enterica subsp. enterica, listeria monocytogenes, escherichia coli o157, vibrio parahaemolyticus, v. vulnificus, campylobacter jejuni, enterobacter sakazakii ... | 2014 | 24328501 |
| in vitro protective efficacy of clostridium butyricum against fish pathogen infections. | most pathogens in intestine are opportunist, called "opportunistic pathogens" that usually do not cause disease in a healthy host. only when the host's resistance is lowered or the intestinal microecological balance is destroyed, the opportunistic pathogens are capable of causing disease. here, two opportunistic pathogens, salmonella enteritidis and vibrio parahaemolyticus were chosen to test the possible antagonistic effect of the probiotic agent clostridium butyricum on these pathogens infecti ... | 2013 | 24426150 |
| evaluation of ice slurries as a control for postharvest growth of vibrio spp. in oysters and potential for filth contamination. | raw oyster consumption is the most common route of exposure for vibrio spp. infections in humans. vibriosis has been increasing steadily in the united states despite efforts to reduce the incidence of the disease. research has demonstrated that ice is effective in reducing postharvest vibrio spp. growth in oysters but has raised concerns of possible contamination of oyster meat by filth (as indicated by the presence of fecal coliform bacteria or clostridium perfringens). this study examined the ... | 2015 | 26197291 |
| disease-outcome trees, eq-5d scores, and estimated annual losses of quality-adjusted life years (qalys) for 14 foodborne pathogens in the united states. | measures of disease burden such as quality-adjusted life years (qalys) are increasingly important to risk-based food safety policy. they provide a means of comparing relative risk from diverse health outcomes. we present detailed disease-outcome trees and eq-5d scoring for 14 major foodborne pathogens representing over 95% of foodborne illnesses, hospitalizations, and deaths due to specified agents in the united states (campylobacter spp., clostridium perfringens, cryptosporidium parvum, cyclosp ... | 2014 | 24588853 |
| debaryomyces hansenii up regulates superoxide dismutase gene expression and enhances the immune response and survival in pacific red snapper (lutjanus peru) leukocytes after vibrio parahaemolyticus infection. | application of yeast is increasing to improve welfare and promotes growth in aquaculture. the halotolerant yeast debaryomyces hansenii is normally a non-pathogenic yeast with probiotic properties and potential source of antioxidant enzymes as superoxide dismutase. here, first, we characterized the sequence features of mnsod and iccu/znsod from pacific red snapper, and second, we evaluated the potential antioxidant immune responses of the marine yeast debaryomyces hansenii strain cbs004 in leukoc ... | 2017 | 28126556 |
| a new zebrafish model of oro-intestinal pathogen colonization reveals a key role for adhesion in protection by probiotic bacteria. | the beneficial contribution of commensal bacteria to host health and homeostasis led to the concept that exogenous non-pathogenic bacteria called probiotics could be used to limit disease caused by pathogens. however, despite recent progress using gnotobiotic mammal and invertebrate models, mechanisms underlying protection afforded by commensal and probiotic bacteria against pathogens remain poorly understood. here we developed a zebrafish model of controlled co-infection in which germ-free zebr ... | 2012 | 22911651 |
| dna shuffling approach for recombinant polyvalent ompas against v. alginolyticus and e. tarda infections. | molecular breeding via dna shuffling directs the evolution of vaccines with desired traits. in the present study, polyvalent ompa vaccines were generated by dna shuffling of five ompa genes from four species of bacteria vibrio parahaemolyticus, v. alginolyticus, edwardsiella tarda and escherichia coli. first, a new hybrid ompa was constructed using va0764 primers and used for construction of a prokaryotic expressing library pompas-fv containing 84 ompas, which were validated by pcr and sds/page. ... | 2016 | 27697557 |
| construction and immune protection evaluation of recombinant polyvalent ompas derived from genetically divergent ompa by dna shuffling. | a wide variety of bacterial infections is a major challenge in aquaculture. development of polyvalent vaccines that can fight against as many pathogens as possible is especially necessary. the present study uses dna shuffling to create a new hybrid ompa with improved cross-protection against vibrio alginolyticus and edwardsiella tarda through the recombination of six ompa genes from vibrio parahaemolyticus, v. alginolyticus, e. tarda and escherichia coli. out of the 43 recombinant chimeras genes ... | 2016 | 26707781 |
| exogenous alanine and/or glucose plus kanamycin kills antibiotic-resistant bacteria. | multidrug-resistant bacteria are an increasingly serious threat to human and animal health. however, novel drugs that can manage infections by multidrug-resistant bacteria have proved elusive. here we show that glucose and alanine abundances are greatly suppressed in kanamycin-resistant edwardsiella tarda by gc-ms-based metabolomics. exogenous alanine or glucose restores susceptibility of multidrug-resistant e. tarda to killing by kanamycin, demonstrating an approach to killing multidrug-resista ... | 2015 | 25651179 |
| daily variations in pathogenic bacterial populations in a monsoon influenced tropical environment. | changing climatic conditions have influenced the monsoon pattern in recent years. variations in bacterial population in one such tropical environment were observed everyday over two years and point out intra and inter annual changes driven by the intensity of rainfall. vibrio spp. were abundant during the monsoon and so were faecal coliforms. vibrio alginolyticus were negatively influenced by nitrate, whereas, silicate and rainfall positively influenced vibrio parahaemolyticus numbers. it is als ... | 2015 | 25956443 |
| sample preparation methods for quantitative detection of dna by molecular assays and marine biosensors. | the need for quantitative molecular methods is growing in environmental, food, and medical fields but is hindered by low and variable dna extraction and by co-extraction of pcr inhibitors. dna extracts from enterococcus faecium, seawater, and seawater spiked with e. faecium and vibrio parahaemolyticus were tested by qpcr for target recovery and inhibition. conventional and novel methods were tested, including synchronous coefficient of drag alteration (scoda) and lysis and purification systems u ... | 2013 | 23790450 |
| plesiomonas shigelloides periprosthetic knee infection after consumption of raw oysters. | periprosthetic infections are a leading cause of morbidity after total joint arthroplasty. common pathogens include staphylococcus aureus, streptococcus, enterococcus, escherichia coli, and pseudomonas aeruginosa. however, there are many cases in which rare bacteria are isolated. this case report describes a periprosthetic knee infection caused by plesiomonas shigelloides. in the united states, p shigelloides and 2 other vibrionaceae family members, vibrio vulnificus and vibrio parahaemolyticus, ... | 2017 | 28235124 |
| microbiological quality and prevalence of β-lactam antibiotic resistance genes in oysters ( crassostrea rhizophorae ). | the microbiological quality of oysters reflects the microbiological quality of their habitats because they are filter feeders. the objective of this study was to assess the bacterial composition of the edible oyster crassostrea rhizophorae in urban and preserved estuaries. particularly, we assessed the presence of pathogenic bacteria, investigated antibiotic susceptibility in bacterial isolates, and quantified β-lactam antibiotic resistance genes (blatem, blashv, and blakpc) via quantitative pcr ... | 2017 | 28207310 |