| rapid detection and monitoring therapeutic efficacy of mycobacterium tuberculosis complex using a novel real-time assay. | we combined real-time rt-pcr and real-time pcr (r/p) assays using a hydrolysis probe to detect mycobacterium tuberculosis complex (mtbc)-specific 16s rrna and its rrna gene (rdna). the assay was applied to 28 nonrespiratory and 207 respiratory specimens from 218 patients. total nucleic acids (including rna and dna) were extracted from samples, and results were considered positive if the repeat rt-pcr threshold cycle was < or =35 and the ratio of real-time rt-pcr and real-time pcr load was > or = ... | 2012 | 22814507 |
| "spoligoriftyping," a dual-priming-oligonucleotide-based direct-hybridization assay for tuberculosis control with a multianalyte microbead-based hybridization system. | we developed "spoligoriftyping," a 53-plex assay based on two preexisting methods, the spoligotyping and "rifoligotyping" assays, by combining them into a single assay. spoligoriftyping allows simultaneous spoligotyping (i.e., clustered regularly interspaced short palindromic repeat [crispr]-based genotyping) and characterization of the main rifampin drug resistance mutations on the rpob hot spot region in a few hours. this test partly uses the dual-priming-oligonucleotide (dpo) principle, which ... | 2012 | 22814456 |
| molecular genotyping of mycobacterium tuberculosis in mie prefecture, japan, using variable numbers of tandem repeats analysis. | the variable numbers of tandem repeats (vntr) analysis is a method frequently employed as a molecular epidemiological tool for mycobacterium tuberculosis genetic fingerprinting. in this study, we characterized the population of m. tuberculosis circulating in mie prefecture, japan, and assessed the utility of the proposed jata12- and 15-vntr analyses of 158 m. tuberculosis clinical isolates using 25 vntr loci. the results revealed that the ancient beijing sublineage is the most prevalent m. tuber ... | 2012 | 22814161 |
| rapid identification of mycobacterium tuberculosis in bactec mgit960 cultures by in-house loop-medicated isothermal amplification. | definitive diagnosis of tuberculosis (tb) by conventional culture, followed by bacterial identification based on biochemical tests is time-consuming and tedious. simple loop-mediated isothermal amplification (lamp) specific for mycobacterium tuberculosis complex, targeting the m. tuberculosis 16s ribosomal rna gene, termed tb-lamp, was evaluated as an alternative for rapid culture confirmation. tb-lamp was assessed for its ability to detect m. tuberculosis complex in bactec mgit 960-positive cul ... | 2012 | 22814152 |
| notes from the field: tuberculosis cluster associated with homelessness--duval county, florida, 2004-2012. | despite a decrease in incidence of tuberculosis (tb) in duval county, florida, from 102 cases (11.2 per 100,000 population) in 2008 to 71 cases (8.2 per 100,000) in 2011,* analysis of mycobacterium tuberculosis genotyping data revealed a substantial increase in the percentage of tb cases with the same genotype.† that percentage increased from 27% (10 of 37) of genotyped cases in 2008 to 51% (30 of 59) of genotyped cases in 2011 (florida department of health, unpublished data, 2012). during this ... | 2012 | 22810268 |
| mycobacterium tuberculosis directs t helper 2 cell differentiation by inducing interleukin-1β production in dendritic cells. | mycobacterium tuberculosis, the causative agent of tuberculosis (tb), resides and replicates within phagocytes and persists in susceptible hosts by modulating protective innate immune responses. furthermore, m. tuberculosis promotes t helper 2 (th2) immune responses by altering the balance of t cell polarizing cytokines in infected cells. however, cytokines that regulate th2 cell differentiation during tb infection remain unknown. here we show that il-1β, produced by phagocytes infected by virul ... | 2012 | 22810226 |
| meningitis and encephalitis. | : neurologists have a vital role in the recognition of meningitis and encephalitis, the accurate evaluation and interpretation of csf studies, and the management and prevention of the neurologic complications of cns infectious diseases. | 2011 | 22809979 |
| heterologous expression and biochemical characterization of recombinant alpha phosphoglucomutase from mycobacterium tuberculosis h37rv. | phosphoglucomutase (pgm) plays an important role in polysaccharide capsule formation and virulence in a number of bacterial pathogens. however, the enzyme has not yet been characterized from mycobacterium tuberculosis (mtb). here, we report the biochemical properties of recombinant mtb-pgm as well as the in silico structural analysis from mtb h37rv. the purified recombinant enzyme was enzymatically active with a specific activity of 67.5 u/mg and experimental k(cat) of 70.31 s(-1) for the substr ... | 2012 | 22809717 |
| c-terminal heat shock protein 70 of mycobacterium tuberculosis as a molecular adjuvant for dna vaccination with the porcine circovirus type 2 orf2 (capsid) gene in mice. | mycobacterium tuberculosis heat shock protein 70 (hsp70) and the peptide binding c-terminal portion of hsp70 (amino acids 359-610; hsp70c) exert an adjuvant effect when used in vaccines. to enhance the immunogenicity of a dna vaccine against porcine circovirus type 2 (pcv2), recombinant plasmids encoding the pcv2 orf2 (capsid) gene fused to full length hsp70 (pca-tch) or truncated c-terminal hsp70c (pca-tchc) were constructed. immunisation of mice with pca-tchc induced higher serum immunoglobuli ... | 2013 | 22809463 |
| physical and functional interactions between 3-methyladenine dna glycosylase and topoisomerase i in mycobacteria. | dna glycosylases play important roles in dna repair in a variety of organisms, including humans. however, the function and regulation of these enzymes in the pathogenic bacterium mycobacterium tuberculosis and related species are poorly understood. in the present study, the physical and functional interactions between 3-methyladenine dna glycosylase (mag) and topoisomerase i (topa) in m. tuberculosis and m. smegmatis were characterized. mag was found to inhibit the function of topa in relaxing s ... | 2012 | 22809157 |
| influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria. | artificial generation of oxygen superoxide radicals in actively growing cultures of mycobacterium tuberculosis, myc. smegmatis, and corynebacterium ammoniagenes is followed by accumulation in the bacterial cells of substantial amounts of 2-c-methyl-d-erythritol-2,4-cyclodiphosphate (mecdp) - an intermediate of the non-mevalonate pathway of isoprenoid biosynthesis (mep) - most possibly due to the interaction of the oxygen radicals with the 4fe-4s group in the active center and inhibition of the e ... | 2012 | 22809155 |
| a genome-wide regulator-dna interaction network in the human pathogen mycobacterium tuberculosis h37rv. | transcription regulation translates static genome information to dynamic cell behaviors, making it central to understand how cells interact with and adapt to their environment. however, only a limited number of transcription regulators and their target genes have been identified in the pathogen mycobacterium tuberculosis , which has greatly impeded our understanding of its pathogenesis and virulence. in this study, we constructed a genome-wide transcription regulatory network of m. tuberculosis ... | 2012 | 22808930 |
| study of rifampicin and isoniazid resistance mutation genes of m. tuberculosis isolates in nepal. | we studied the rifampicin (rif) and isoniazid (inh) resistant genes in mycobacterium tuberculosis isolated from sputum samples of re-treated tb cases using phenotypic drug susceptibility test (dst) and rapid molecular method (genotype; mtbdr plus, hains life science, nehren, germany). of the total 207 isolates included in this study, 90 (42.0%) were rif resistant, 107 (50.7%) were inh resistant whereas 86 (41.5%) were multidrug resistant (mdr) by dst. according to genotypic analysis, 37.0% (77/2 ... | 2011 | 22808802 |
| bat3 regulates mycobacterium tuberculosis protein esat-6-mediated apoptosis of macrophages. | hla-b-associated transcript 3 (bat3), also known as scythe or bag6, is a nuclear protein implicated in the control of apoptosis and natural killer (nk) cell-dendritic cell (dc) interaction. we demonstrate that bat3 modulates the immune response by regulating the function of macrophages. bat3 is released by macrophages in vitro and it down-regulates nitric oxide and proinflammatory cytokines release in ifn-γ and lps stimulated macrophages. furthermore, mycobacterium tuberculosis-derived protein e ... | 2012 | 22808273 |
| multidrug-resistant tuberculosis in admitted patients at a tertiary referral hospital of bangladesh. | this study was set out to investigate the magnitude, patterns and molecular characterization of drug-resistant mycobacterium tuberculosis strains at a tertiary referral hospital in bangladesh. | 2012 | 22808189 |
| crowd sourcing a new paradigm for interactome driven drug target identification in mycobacterium tuberculosis. | a decade since the availability of mycobacterium tuberculosis (mtb) genome sequence, no promising drug has seen the light of the day. this not only indicates the challenges in discovering new drugs but also suggests a gap in our current understanding of mtb biology. we attempt to bridge this gap by carrying out extensive re-annotation and constructing a systems level protein interaction map of mtb with an objective of finding novel drug target candidates. towards this, we synergized crowd sourci ... | 2012 | 22808064 |
| genetic diversity of the mycobacterium tuberculosis beijing family based on snp and vntr typing profiles in asian countries. | the mycobacterium tuberculosis (mtb) beijing strain is highly virulent, drug resistant, and endemic over asia. to explore the genetic diversity of this family in several different regions of eastern asia, 338 beijing strains collected in taiwan (republic of china) were analyzed by mycobacterial interspersed repetitive unit-variable number tandem repeat (miru-vntr) typing and compared with published miru-vntr profiles and by the hunter-gaston diversity index (hgdi) of beijing strains from japan a ... | 2012 | 22808061 |
| world health organization group 5 drugs for the treatment of drug-resistant tuberculosis: unclear efficacy or untapped potential? | treatment of multidrug-resistant or extensively drug-resistant tuberculosis (dr-tuberculosis) is challenging because commonly used second-line drugs are poorly efficacious and highly toxic. although world health organization group 5 drugs are not recommended for routine use because of unclear activity, some may have untapped potential as more efficacious or better tolerated alternatives. | 2013 | 22807518 |
| computational screening for new inhibitors of m. tuberculosis mycolyltransferases antigen 85 group of proteins as potential drug targets. | the group of antigen 85 proteins of mycobacterium tuberculosis is responsible for converting trehalose monomycolate to trehalose dimycolate, which contributes to cell wall stability. here, we have used a serial enrichment approach to identify new potential inhibitors by searching the libraries of compounds using both 2d atom pair descriptors and binary fingerprints followed by molecular docking. three different docking softwares autodock, gold, and ligandfit were used for docking calculations. i ... | 2013 | 22804492 |
| rationalization and prediction of drug resistant mutations in targets for clinical anti-tubercular drugs. | resistance to therapy limits the effectiveness of drug treatment in many diseases. drug resistance can be considered as a successful outcome of the bacterial struggle to survive in the hostile environment of a drug-exposed cell. an important mechanism by which bacteria acquire drug resistance is through mutations in the drug target. drug resistant strains (multi-drug resistant and extensively drug resistant) of mycobacterium tuberculosis are being identified at alarming rates, increasing the glo ... | 2013 | 22803837 |
| the safety of ustekinumab treatment in patients with moderate-to-severe psoriasis and latent tuberculosis infection. | ustekinumab is a monoclonal antibody that targets interleukin (il)-12/23 p40 to treat psoriasis. the il-12 pathway is also important in regulating immunity to mycobacterium tuberculosis. | 2012 | 22803615 |
| consequences of noncompliance for therapy efficacy and emergence of resistance in murine tuberculosis caused by the beijing genotype of mycobacterium tuberculosis. | despite great effort by health organizations worldwide in fighting tuberculosis (tb), morbidity and mortality are not declining as expected. one of the reasons is related to the evolutionary development of mycobacterium tuberculosis, in particular the beijing genotype strains. in a previous study, we showed the association between the beijing genotype and an increased mutation frequency for rifampin resistance. in this study, we use a beijing genotype strain and an east-african/indian genotype s ... | 2012 | 22802244 |
| effect of vitamin d3 on chemokine expression in pulmonary tuberculosis. | 1,25 dihydroxy vitamin d(3) (vitamin d(3)) is an immunomodulator and its deficiency has been associated with susceptibility to tuberculosis. we have studied the immunoregulatory role of vitamin d(3) on various chemokine expression in pulmonary tuberculosis. peripheral blood mononuclear cells obtained from 21 pulmonary tuberculosis (ptb) patients and 24 healthy controls (hcs) were cultured for 48 h with culture filtrate antigen (cfa) of mycobacterium tuberculosis with or without vitamin d(3) at a ... | 2012 | 22800603 |
| a new antimycobacterial furanolignan from leucophyllum frutescens. | bioassay-guided fractionation of the minor active fractions obtained from the root bark of leucophyllumfrutescens (berl.) i. m. johnst. led to isolation from the n-hexane extract of a new compound with moderate activity against the h37rv mycobacterium tuberculosis strain (mic 63 microg/ml), and low cytotoxicity, as shown by the ic5o against vero cells. the compound was identified by 1d/2d nmr spectroscopy a s2',5"-dimethoxysesamin. | 2012 | 22799084 |
| variability of infectious aerosols produced during coughing by patients with pulmonary tuberculosis. | mycobacterium tuberculosis is transmitted by infectious aerosols, but assessing infectiousness currently relies on sputum microscopy that does not accurately predict the variability in transmission. | 2012 | 22798319 |
| rapid and accurate identification of mycobacterium tuberculosis complex and common non-tuberculous mycobacteria by multiplex real-time pcr targeting different housekeeping genes. | rapid and accurate identification of mycobacteria isolates from primary culture is important due to timely and appropriate antibiotic therapy. conventional methods for identification of mycobacterium species based on biochemical tests needs several weeks and may remain inconclusive. in this study, a novel multiplex real-time pcr was developed for rapid identification of mycobacterium genus, mycobacterium tuberculosis complex (mtc) and the most common non-tuberculosis mycobacteria species includi ... | 2012 | 22797866 |
| antimycobacterial activity of constituents from foeniculum vulgare var. dulce grown in mexico. | bioassay guided fractionation of an antimycobacterial extract of foeniculum vulgare var dulce (apiaceae) led to the isolation and characterization of 5-hydroxyfurano-coumarin. the chemical structure of this compound was elucidated by 1h and 13c (1d and 2d) nuclear magnetic resonance (nmr) spectroscopy. in addition, the active fractions were analyzed by gc-ms and seventy eight compounds were identified; the major compounds were 1,3-benzenediol, 1-methoxycyclohexene, o-cymene, sorbic acid, 2-hydro ... | 2012 | 22797778 |
| tnf-α/il-2 ratio discriminates latent from active tuberculosis in immunocompetent children: a pilot study. | distinguishing latent tuberculosis (ltb) from tuberculosis (tb) disease may be challenging in children. here, we analyzed cytokine profiles that can distinguish the two infection stages in a nonendemic country (france). | 2012 | 22797138 |
| development of a selective activity-based probe for adenylating enzymes: profiling mbta involved in siderophore biosynthesis from mycobacterium tuberculosis. | mbta is an adenylating enzyme from mycobacterium tuberculosis that catalyzes the first step in the biosynthesis of the mycobactins. a bisubstrate inhibitor of mbta (sal-ams) was previously described that displays potent antitubercular activity under iron-replete as well as iron-deficient growth conditions. this finding is surprising since mycobactin biosynthesis is not required under iron-replete conditions and suggests off-target inhibition of additional biochemical pathways. as a first step to ... | 2012 | 22796950 |
| use of multiplex allele-specific polymerase chain reaction (mas-pcr) to detect multidrug-resistant tuberculosis in panama. | the frequency of individual genetic mutations conferring drug resistance (dr) to mycobacterium tuberculosis has not been studied previously in central america, the place of origin of many immigrants to the united states. the current gold standard for detecting multidrug-resistant tuberculosis (mdr-tb) is phenotypic drug susceptibility testing (dst), which is resource-intensive and slow, leading to increased mdr-tb transmission in the community. we evaluated multiplex allele-specific polymerase c ... | 2012 | 22792333 |
| structure-function relationships of the mycobacterium tuberculosis transcription factor whib1. | members of the whib-like (wbl) protein family possess iron-sulfur clusters and are implicated in the regulation of developmental processes in actinomycetes. mycobacterium tuberculosis possesses seven wbl proteins. the [4fe-4s] cluster of m. tuberculosis whib1 is relatively insensitive to o(2) but very sensitive to nitric oxide (no). nitric oxide nitrosylates the whib1 iron-sulfur cluster and promotes dna-binding; the apo-forms of whib1 also bind dna. however, the molecular requirements for iron- ... | 2012 | 22792304 |
| macrophages from elders are more permissive to intracellular multiplication of mycobacterium tuberculosis. | the elderly account for a disproportionate share of all tuberculosis cases, and the population ageing may not fully explain this phenomenon. we have performed in vitro infection experiments to investigate whether there is an immunological basis for the apparent susceptibility of elders to tuberculosis. in our infection model, mycobacterium tuberculosis induces a higher production of interleukin (il)-6 and reactive oxygen species in macrophages from elders than from younger adults. this response ... | 2013 | 22791369 |
| enzymatic reduction of 9-methoxytariacuripyrone by saccharomyces cerevisiae and its antimycobacterial activity. | biotransformation processes have been successfully utilized to obtain products of pharmaceutical, chemical, food, and agricultural interest, which are difficult to obtain by classic chemical methods. the compound with antituberculous activity, 9-methoxy-tariacuripyrone (1), isolated from aristolochia brevipes, was submitted to biotransformation with the yeast saccharomyces cerevisiae under culture, yielding 5-amino-9-methoxy-3,4-dihydro-2h-benzo[h]chromen-2-one (2). the structure of (2) was eluc ... | 2012 | 22790562 |
| cns-immune reconstitution inflammatory syndrome in the setting of hiv infection, part 2: discussion of neuro-immune reconstitution inflammatory syndrome with and without other pathogens. | while the previous review of cns-iris in the hiv-infected patient on highly active antiretroviral therapy (part 1) dealt with an overview of the biology, pathology, and neurologic presentation of this condition and a discussion of the atypical imaging findings in pml-iris and cryptococcal meningitis-iris due to the robust inflammatory response, the current review (part 2) discusses the imaging findings in other commonly encountered organisms seen in association with cns-iris, namely, vzv, cmv, h ... | 2013 | 22790252 |
| comparison of two in-house real-time pcr assays with mtb q-pcr alert and genotype mtbdrplus for the rapid detection of mycobacteria in clinical specimens. | an in-house is6110 real-time pcr (ih is6110), mtb q-pcr alert (q-pcr) and genotype mtbdrplus (mtbdr; hain lifescience) were compared for the direct detection of mycobacterium tuberculosis complex (mtbc) in 87 specimens following automated nuclisens easymag dna extraction. this included 82 first smear-positive specimens and three smear-negative specimens. another in-house real-time pcr with a mycobacterium genus-specific probe for the internal transcribed spacer (its) region (ih its) was used to ... | 2012 | 22790204 |
| the immunological life cycle of tuberculosis. | immune responses to mycobacterium tuberculosis are only partially effective; they drive the bacteria into a latent state, but rarely eliminate them. unfortunately, the latent state of m. tuberculosis is reversible, and reactivation tuberculosis is the source of most transmission. studies in animal models and in humans have not yet yielded a comprehensive picture of the mechanisms or correlates of immunity to m. tuberculosis infection, or of why immunity fails to eradicate the pathogen. this revi ... | 2012 | 22790178 |
| pre-xdr & xdr in mdr and ofloxacin and kanamycin resistance in non-mdr mycobacterium tuberculosis isolates. | resistance to second line anti tubercular drugs is a cause of serious concern. the present study reports the prevalence of ofloxacin (ofx) and kanamycin (km) resistance in mycobacterium tuberculosis isolates from cases of pulmonary tuberculosis, who received anti tubercular treatment for >4 weeks in past. of total 438 enrolled patients, 361 were culture positive for m. tuberculosis, of which, 95 (26.3%) were ofx resistant & 49 (13.5%) were km resistant. total 130 isolates were multidrug resistan ... | 2012 | 22789499 |
| genetic engineering of mycobacterium tuberculosis: a review. | genetic engineering has been used for decades to mutate and delete genes in the mycobacterium tuberculosis genome with the translational goal of producing attenuated mutants with conserved susceptibility to antituberculous antibiotics. the development of plasmids and mycobacteriophages that can transfer dna into the m. tuberculosis chromosome has effectively overcome m. tuberculosis slow growth rate and the capsule and mycolic acid wall, which limit dna uptake. the use of genetic engineering tec ... | 2012 | 22789498 |
| hepatic tuberculosis presenting with extreme hyperferritinemia masquerading as adult-onset still's disease: a case report. | isolated hepatic tuberculosis is an uncommon manifestation of one of the most common infections worldwide, caused by mycobacterium tuberculosis. extremely high serum ferritin, which is regarded as a marker of adult onset still's disease, has not been observed in patients with tuberculosis of the liver. we report a case of hepatic tuberculosis who presented with clinical criteria of adult-onset still's disease and extreme hyperferritinemia, which posed a diagnostic confusion. | 2012 | 22788693 |
| immunotherapy for tb. | mycobacterium tuberculosis was one of the first human pathogens to be identified as the cause of a specific disease--tb. tb was also one of the first specific diseases for which immunotherapy was attempted. in more than a century since, multiple different immunotherapies have been attempted, alongside vaccination and antibiotic treatment, with varying degrees of success. despite this, tb remains a major worldwide health problem that causes nearly 2 million deaths annually and has infected an est ... | 2012 | 22788130 |
| transmission of mycobacterium orygis (m. tuberculosis complex species) from a tuberculosis patient to a dairy cow in new zealand. | mycobacterium orygis, previously called the oryx bacillus, is a member of the mycobacterium tuberculosis complex and has been reported only recently as a cause of human tuberculosis in patients of south asian origin. we present the first case documenting the transmission of this organism from a human to a cow. | 2012 | 22785186 |
| growth of mycobacteria in urine determined by isothermal microcalorimetry: implications for urogenital tuberculosis and other mycobacterial infections. | to overcome the limitations of current urine-based diagnostic assays of urogenital tuberculosis, we used isothermal microcalorimetry to detect the metabolic activity of mycobacterium tuberculosis and other commonly neglected pathogenic mycobacteria in urine and accurately determine their growth parameters. | 2012 | 22784494 |
| mincle is not essential for controlling mycobacterium tuberculosis infection. | individually and combined, toll-like receptors (tlr)-2, -4, -9, nucleotide oligomerization domain (nod) 2 and nalp3 contribute to the mycobacterium tuberculosis (mtb)-induced innate immune response only to a limited extent, particularly in terms of inducing antibacterial protection and granuloma formation in vivo. a singular essential sensory component of this initial response has not been discovered yet. trehalose-6,6'-dimycolate (tdm), a well known mycobacterial cell wall glycolipid, is believ ... | 2013 | 22784441 |
| development of a new dna extraction protocol for pfge typing of mycobacterium tuberculosis complex. | a modified pulsed-field gel electrophoresis (pfge) protocol was developed and applied to clinical isolates of mycobacterium tuberculosis complex to reduce the cost of using lyticase. this protocol reduces the expense of pfge typing of mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria. | 2012 | 22783461 |
| comprehensive tuberculosis screening program in patients with inflammatory arthritides treated with golimumab, a human anti-tumor necrosis factor antibody, in phase iii clinical trials. | reactivation of mycobacterium tuberculosis infection is a major complication in patients treated with anti-tumor necrosis factor (anti-tnf) agents. we report on the 5 cases of active tuberculosis (tb) that developed in the golimumab phase iii program (3 with rheumatoid arthritis, 1 with psoriatic arthritis, and 1 with ankylosing spondylitis) through 1 year among 2,210 patients receiving golimumab. | 2013 | 22782640 |
| [advances in the research of rapid detection methods of pyrazinamide-resistant mycobacterium tuberculosis]. | | 2012 | 22781157 |
| mycobacterium tuberculosis whib4 regulates oxidative stress response to modulate survival and dissemination in vivo. | host-generated oxidative stress is considered one of the main mechanisms constraining mycobacterium tuberculosis (mtb) growth. the redox-sensing mechanisms in mtb are not completely understood. here we show that whib4 responds to oxygen (o₂) and nitric oxide (no) via its 4fe-4s cluster and controls the oxidative stress response in mtb. the whib4 mutant (mtbδwhib4) displayed an altered redox balance and a reduced membrane potential. microarray analysis demonstrated that mtbδwhib4 overexpresses th ... | 2012 | 22780904 |
| determination of principal genotypic groups among susceptible, mdr and xdr clinical isolates of mycobacterium tuberculosis in belarus and iran. | all members of the mycobacterium tuberculosis complex were assigned to one of the three principle genetic groups based on katg463/gyra95 polymorphism. | 2012 | 22779936 |
| rapid diagnosis of tuberculosis and multidrug resistance using a mgit 960 system. | the purpose of this study was to compare the turnaround time for liquid culturing and primary anti-tuberculous drug susceptibility testing (dst) performed using the mycobacteria growth indicator tube (mgit) 960 system (becton dickinson, usa) with that for conventional culturing and dst (by the absolute concentration method) performed using solid culture medium and to determine the concordance rates of dst results obtained using these 2 methods. | 2012 | 22779067 |
| clinical isolates of mycobacterium tuberculosis differ in their ability to induce respiratory burst and apoptosis in neutrophils as a possible mechanism of immune escape. | tuberculosis pathogenesis was earlier thought to be mainly related to the host but now it appears to be clear that bacterial factors are also involved. genetic variability of mycobacterium tuberculosis (mtb) could be slight but it may lead to sharp phenotypic differences. we have previously reported that nonopsonized mtb h37rv induce apoptosis of polymorphonuclear neutrophils (pmns) by a mechanism that involves the p38 pathway. here we evaluated the capability to induce pmn apoptosis of two prev ... | 2012 | 22778761 |
| human tollip regulates tlr2 and tlr4 signaling and its polymorphisms are associated with susceptibility to tuberculosis. | tuberculosis, one of the leading causes of death worldwide, stimulates inflammatory responses with beneficial and pathologic consequences. the regulation and nature of an optimal inflammatory response to mycobacterium tuberculosis remains poorly understood in humans. insight into mechanisms of negative regulation of the tlr-mediated innate immune response to m. tuberculosis could provide significant breakthroughs in the design of new vaccines and drugs. we hypothesized that tollip and its common ... | 2012 | 22778396 |
| arylamine n-acetyltransferases--from drug metabolism and pharmacogenetics to identification of novel targets for pharmacological intervention. | arylamine n-acetyltransferases (nats) are defined as xenobiotic metabolizing enzymes, adding an acetyl group from acetyl coenzyme a (coa) to arylamines and arylhydrazines. nats are found in organisms from bacteria and fungi to vertebrates. several isoenzymes, often polymorphic, may be present in one organism. there are two functional polymorphic nats in humans and polymorphisms in nat2 underpinned pharmacogenetics as a discipline. nat enzymes have had a role in important metabolic concepts: the ... | 2012 | 22776642 |
| comparative analysis of mycobacterial truncated hemoglobin promoters and the groel2 promoter in free-living and intracellular mycobacteria. | the success of mycobacterium tuberculosis depends on its ability to withstand and survive the hazardous environment inside the macrophages that are created by reactive oxygen intermediates, reactive nitrogen intermediates, severe hypoxia, low ph, and high co(2) levels. therefore, an effective detoxification system is required for the pathogen to persist in vivo. the genome of m. tuberculosis contains a new family of hemoproteins named truncated hemoglobin o (trhbo) and truncated hemoglobin n (tr ... | 2012 | 22773641 |
| tuberculosis: finding a new potential antimycobacterium derivative in a aldehyde-arylhydrazone-oxoquinoline series. | tuberculosis (tb) is a contagious disease caused by mycobacterium tuberculosis, which remains a serious public health problem. the emergence of resistant bacterial strains has continuously increased and new treatment options are currently in need. in this work, we identified a new potential aldehyde-arylhydrazone-oxoquinoline derivative (4e) with interesting chemical structural features that may be important for designing new anti-tb agents. this 1-ethyl-n'-[(1e)-(5-nitro-2-furyl)methylene]-4-ox ... | 2012 | 22773252 |
| non-invasive diagnosis of lung tuberculosis in children by single voxel ¹h-magnetic resonance spectroscopy. | our previous study showed that (1)h-magnetic resonance spectroscopy ((1)h-mrs) can detect lipid peaks characteristic for mycobacterium tuberculosis infection in cerebral lesions of young children; therefore, we aimed to extend and validate the application of (1)h-mrs for the diagnosis of active pulmonary tuberculosis lesions in three adolescent patients. here, we document lipid peaks characteristic for m. tuberculosis infection by (1)h-mrs from lung tissue surrounding lung cavities of two patien ... | 2012 | 22772834 |
| a new mycobacterium tuberculosis smooth colony reduces growth inside human macrophages and represses pdim operon gene expression. does an heterogeneous population exist in intracellular mycobacteria? | mycobacterium tuberculosis (mtb) colony morphology was associated to the pathogen's virulence. we isolated a new mtb h37rv smooth colony, which only appeared following human macrophages (mdm) infection. the new phenotype was alcohol-acid resistant, but devoid of a covering capsule and biofilm defective. we ascertained that there were no deletions in the rv0096-rv0101 pdim operon, but that its expression was repressed as compared to mtb wild type (wt). its lipid composition displayed lower pdim c ... | 2012 | 22771837 |
| an in-house multiplex pcr test for the detection of mycobacterium tuberculosis, its validation & comparison with a single target tb-pcr kit. | the conventional techniques used in tb diagnosis like afb (acid fast bacilli) smear microscopy lack sensitivity and the gold standard, culture test takes time. a test based on multiplex polymerase chain reaction (pcr) targeting the 38 kda gene and is6110 insertion sequence, specific to mycobacterium tuberculosis was developed to further increase the sensitivity of a tb-pcr kit targeting only 38 kda gene developed earlier in the same laboratory. the multiplex test was validated using sputum sampl ... | 2012 | 22771614 |
| in-house, simple & economical phage technique for rapid detection of rifampicin, isoniazid, ethambutol, streptomycin & ciprofloxacin drug resistance using mycobacterium tuberculosis isolates. | multiple drug resistance (mdr) among mycobacterium tuberculosis poses a serious therapeutic problem. early detection of mdr can be valuable but the conventional drug susceptibility tests take 4-6 wk time after the laboratory isolation of m. tuberculosis. the bacterial phage assay has been reported as a rapid tool for rifampicin susceptibility testing of tubercle bacilli using the suspension of isolated cultures. the present study was aimed to set up a phage assay for testing drug susceptibility ... | 2012 | 22771613 |
| detection of mycobacterium tuberculosis directly from sputum specimens & phenotypic drug resistance pattern of m. tuberculosis isolates from suspected tuberculosis patients in chennai. | mrna is more rapidly destroyed in cells than rrna or genomic dna, an assay targeting bacterial mrna would provide a better guide to mycobacterial viability than amplification tests directed at dna or rrna targets. this study was carried out to standardize reverse transcriptase pcr (rt-pcr) targeting 85b gene for the rapid detection of viable mycobacterium tuberculosis from sputum specimens of suspected tb patients at chennai, south india and to detect mdr-tb circulating in this population. | 2012 | 22771612 |
| footprints of genetic susceptibility to pulmonary tuberculosis: cytokine gene variants in north indians. | tuberculosis is (tb) responsible for high morbidity and mortality worldwide. cytokines play a major role in defense against mycobacterium tuberculosis infection. polymorphisms in the genes encoding the various pro- and anti-inflammatory cytokines have been associated with tuberculosis susceptibility. in this study we examined association of 25 sequence polymorphisms in six candidate cytokine genes namely ifng, tnfb, il4, il1ra, il1b and il12 and their related haplotypes with risk of developing p ... | 2012 | 22771610 |
| variations in the occurrence of specific rpob mutations in rifampicin-resistant mycobacterium tuberculosis isolates from patients of different ethnic groups in kuwait. | frequency of resistance-conferring mutations vary among isoniazid- and ethambutol-resistant mycobacterium tuberculosis isolates obtained from patients of various ethnic groups. this study was aimed to determine the occurrence of specific rpob mutations in rifampicin-resistant m. tuberculosis isolates from tuberculosis patients of various ethnic groups in kuwait. | 2012 | 22771609 |
| whole cell & culture filtrate proteins from prevalent genotypes of mycobacterium tuberculosis provoke better antibody & t cell response than laboratory strain h 37 rv. | the immune responses to different antigens of mycobacterium tuberculosis h 37 rv vary from patient to patient with tuberculosis (tb). therefore, significant difference might be documented between the h 37 rv with long histories of passages and recent clinical isolates of m. tuberculosis. in the present study, immune response of tb patients and healthy controls against 39 clinical m. tuberculosis isolates was correlated with laboratory strain h 37 rv. | 2012 | 22771608 |
| the potential impact of new diagnostic tests on tuberculosis epidemics. | new diagnostic tests for tuberculosis, especially those based on nucleic acid amplification, offer the possibility of early and accurate diagnosis of active tb. in this study we use mathematical modelling to explore the potential epidemiological impact of these new tests, with particular reference to india. | 2012 | 22771607 |
| current tuberculosis diagnostic tools & role of urease breath test. | tuberculosis (tb) remains a significant public health issue worldwide especially in developing countries, where the disease is endemic, and effective tb diagnostic as well as treatment-monitoring tools are serious barriers to defeating the disease. detection of pathogen-specific metabolic pathways offers a potential alternative to current methods, which focus on bacterial growth, bacterial nucleic acid amplification, or detection of host immune response to the pathogen. metabolic pathway detecti ... | 2012 | 22771606 |
| consistency of standard laboratory strain mycobacterium tuberculosis h 37 rv with ethionamide susceptibility testing. | drug susceptibility pattern of standard mycobacterium tuberculosis strain h 37 rv showed discrepancy in minimum inhibitory concentration method for ethionamide and consistent results were obtained for the other second line drugs namely, kanamycin and ofloxacin. it is, therefore, necessary to revisit the susceptibility testing method for ethionamide for effective clinical management of patients with drug resistant tuberculosis. | 2012 | 22771598 |
| combined rpob duplex pcr and hsp65 pcr restriction fragment length polymorphism with capillary electrophoresis as an effective algorithm for identification of mycobacterial species from clinical isolates. | mycobacteria can be quickly and simply identified by pcr restriction-enzyme analysis (pra), but misidentification can occur because of similarities in band sizes that are critical for discriminating among species. capillary electrophoresis can provide computer-aided band discrimination. the aim of this research was to develop an algorithm for identifying mycobacteria by combined rpob duplex pra (dpra) and hsp65 pra with capillary electrophoresis. | 2012 | 22769981 |
| [immune regulation of t-bet adjuvant on ag85b dna vaccine against mycobacterium tuberculosis]. | to construct a novel ag85b dna vaccine based on the genetic adjuvant of t-bet known as th1 transcription factor and to research the immunoregulation function of the dna complex vaccine. | 2012 | 22768853 |
| high resolution discrimination of clinical mycobacterium tuberculosis complex strains based on single nucleotide polymorphisms. | recently, the diversity of the mycobacterium tuberculosis complex (mtbc) population structure has been described in detail. based on geographical separation and specific host pathogen co-evolution shaping mtbc virulence traits, at least 20 major lineages/genotypes have evolved finally leading to a clear influence of strain genetic background on transmissibility, clinical presentation/outcome, and resistance development. therefore, high resolution genotyping for characterization of strains in lar ... | 2012 | 22768315 |
| high-throughput screening and sensitized bacteria identify an m. tuberculosis dihydrofolate reductase inhibitor with whole cell activity. | mycobacterium tuberculosis (mtb), the causative agent of tuberculosis, is a bacterial pathogen that claims roughly 1.4 million lives every year. current drug regimens are inefficient at clearing infection, requiring at least 6 months of chemotherapy, and resistance to existing agents is rising. there is an urgent need for new drugs that are more effective and faster acting. the folate pathway has been successfully targeted in other pathogens and diseases, but has not yielded a lead drug against ... | 2012 | 22768185 |
| resistance to first-line anti-tb drugs is associated with reduced nitric oxide susceptibility in mycobacterium tuberculosis. | the relative contribution of nitric oxide (no) to the killing of mycobacterium tuberculosis in human tuberculosis (tb) is controversial, although this has been firmly established in rodents. studies have demonstrated that clinical strains of m. tuberculosis differ in susceptibility to no, but how this correlates to drug susceptibility and clinical outcome is not known. | 2012 | 22768155 |
| [abdominal tuberculosis in a young immigrant patient: a clinical case]. | in developing countries, tuberculosis (tbc) is commonly associated with inadequate socio-economic and sanitary conditions. currently, in western countries, tbc is often linked with hiv infection, an ageing population or trans-global migration. approximately two out of ten tb cases worldwide are extra-pulmonary, of which abdominal tuberculosis accounts for 11%-16%. the mycobacterium tuberculosis complex involves the abdomen as primary or secondary localization (hematogenous spread or from pulmona ... | 2012 | 22767312 |
| evaluation of bactec mgit 960 system for the second-line drugs susceptibility testing of mycobacterium tuberculosis in china. | when the performance of the bactec mgit 960 system was evaluated with the traditional proportion method (pm) on 321 clinical isolates of mycobacterium tuberculosis from china, concordance values were 98.44% capreomycin, 98.75% kanamycin, 98.75% ofloxacin and 94.08% ethionamide. the turnaround time with bactec mgit 960 system (7.5±1.8 days) was significantly shorter than with pm (28 days or 42 days). therefore, the bactec mgit 960 system was a reliable and rapid method for second-line drug suscep ... | 2012 | 22766324 |
| salicylanilide derivatives block mycobacterium tuberculosis through inhibition of isocitrate lyase and methionine aminopeptidase. | the global burden of tuberculosis, its health and socio-economic impacts, the presence of drug-resistant forms and a potential threat of latent tuberculosis should serve as a strong impetus for the development of novel antituberculosis agents. we reported the in vitro activity of salicylanilide benzoates and pyrazine-2-carboxylates against mycobacterium tuberculosis (minimum inhibitory concentrations as low as 0.5 μmol/l). nineteen salicylanilide derivatives with mostly good antimycobacterial ac ... | 2012 | 22765970 |
| new treatment options for multidrug-resistant tuberculosis. | despite the development of effective treatments, tuberculosis (tb) remains a major health problem. tb continues to infect new victims and kills nearly 2 million people annually. the problem is much greater in resource-limited countries but is present worldwide. inadequate public health resources, cost, the obligatory long treatment period, and adverse drug effects contribute to treatment failures and relapses. drug-resistant mycobacterium tuberculosis (mtb) strains arise spontaneously and are pr ... | 2012 | 22763676 |
| purified protein derivatives of tuberculin--past, present, and future. | the tuberculin skin test, which involves monitoring the immune reaction to an injection of purified protein derivative (ppd), has been the most widely used method for detecting infection with mycobacterium tuberculosis since its development in 1930s. until recently, the molecular composition of ppd was unknown. this thwarted the discovery of improved skin testing reagents and drastically hindered efforts to define the mechanism of action. proteomic evaluation of ppd combined with a detailed anal ... | 2012 | 22762692 |
| the colour test for drug susceptibility testing of mycobacterium tuberculosis strains. | tartu, estonia. | 2012 | 22762424 |
| new insights into fluoroquinolone resistance in mycobacterium tuberculosis: functional genetic analysis of gyra and gyrb mutations. | fluoroquinolone antibiotics are among the most potent second-line drugs used for treatment of multidrug-resistant tuberculosis (mdr tb), and resistance to this class of antibiotics is one criterion for defining extensively drug resistant tuberculosis (xdr tb). fluoroquinolone resistance in mycobacterium tuberculosis has been associated with modification of the quinolone resistance determining region (qrdr) of gyra. recent studies suggest that amino acid substitutions in gyrb may also play a cruc ... | 2012 | 22761889 |
| adjunctive tnf inhibition with standard treatment enhances bacterial clearance in a murine model of necrotic tb granulomas. | it has been hypothesized that early host-responses during tb treatment may paradoxically promote survival of persistent bacteria. we therefore evaluated whether adjunctive inhibition of tumor necrosis factor alpha (tnf-α)-a key cytokine in host responses against tb-could hasten bacterial clearance in a mouse strain that develops necrotic lesions in response to mycobacterium tuberculosis infection. | 2012 | 22761866 |
| a model-based bayesian estimation of the rate of evolution of vntr loci in mycobacterium tuberculosis. | variable numbers of tandem repeats (vntr) typing is widely used for studying the bacterial cause of tuberculosis. knowledge of the rate of mutation of vntr loci facilitates the study of the evolution and epidemiology of mycobacterium tuberculosis. previous studies have applied population genetic models to estimate the mutation rate, leading to estimates varying widely from around 10⁻⁵ to 10⁻² per locus per year. resolving this issue using more detailed models and statistical methods would lead t ... | 2012 | 22761563 |
| cutaneous tuberculosis caused by isoniazid-resistant mycobacterium tuberculosis. | cutaneous tuberculosis constitutes an uncommon presentation of extrapulmonary tuberculosis, especially among immune-competent patients. here, there is a report of a 38-year-old man who presented with non-pruritic skin plaques, caused by isoniazid-resistant mycobacterium tuberculosis, over his buttock, inguinal area and earlobe. | 2012 | 22761237 |
| [erythema induratum of bazin associated to mycobacterium tuberculosis infection]. | erythema induratum of bazin is uncommon in children. we report the case of a 12 year-old girl from china, referred by her pediatrician with long lasting lesions of erythema nodosum and a positive tuberculin skin test (20 mm). she had no other symptoms. physical examination showed purple nodular lesions located on both legs, mostly on the pretibial areas but also on her calves. a chest x-ray was normal. a skin biopsy was obtained, showing granulomata and septolobular panniculitis. attempts to ide ... | 2012 | 22760758 |
| pediatric tuberculosis: the litmus test for tuberculosis control. | the epidemiology of pediatric tuberculosis (tb) from 1995 to 2000 in harris county, tx, has been previously reported. this study was conducted to evaluate the continued trends of mycobacterium tuberculosis clustering and the role of genotyping in pediatric tb. | 2012 | 22760534 |
| the effect of combination ifn-alpha-2a with usual antituberculosis chemotherapy in non-responding tuberculosis and diabetes mellitus: a case report and review of the literature. | a limited number of publications indicate that certain interferons (ifns) may have a role in difficult-to-treat tuberculosis. we present a 48-year-old male diabetic patient who was referred to our department with the presumptive diagnosis of multidrug resistant tuberculosis. during the previous 8 months, he had been treated initially with a four-drug regimen (rifampicin, isoniazid, pyrazinamide, and ethambutol), which was later modified by the addition of streptomycin and ciprofloxacin, but his ... | 2012 | 22759763 |
| screening and comparison of differentially expressed genes between one mdr-tb strain and the virulent m. tuberculosis h37rv. | to screen and compare the differentially expressed genes between one mdr-tb strain separated from one child patient and the virulent mycobacterium tuberculosis h37rv, suppression subtractive hybridization (ssh) technology was used to build a library of cdnas that were differentially expressed in the mdr and h37rv. from this cdna library, genes that were expressed in the mdr-tb but not in the h37rv were selected for gene sequencing and homology analysis; 113 positive clones were obtained, their c ... | 2012 | 22759522 |
| in silico analysis of dosr regulon proteins of mycobacterium tuberculosis. | one of the challenges faced by mycobacterium tuberculosis (m. tuberculosis) in dormancy is hypoxia. dosr/devr of m. tuberculosis is a two component dormancy survival response regulator which induces the expression of 48 genes. in this study, we have used dosr regulon proteins of m. tuberculosis h37rv as the query set and performed a comprehensive homology search against the non-redundant database. homologs were found in environmental mycobacteria, environmental bacteria and archaebacteria. analy ... | 2012 | 22759512 |
| isolated tubercular liver abscess in an elderly diabetic successfully treated with systemic antitubercular drugs. | tubercular liver abscess is a rare extrapulmonary manifestation of tuberculosis. we are presenting a case of isolated tubercular liver abscess in a 70 year old diabetic male without any evidence of tuberculosis in the lungs or abdomen. diagnosis was made on the basis of radiological findings along with pcr for mycobacterium tuberculosis in pus aspirated from abscess under ct guidance. systemic antitubercular drugs were given for 6 months. on follow up patient improved clinically with radiologica ... | 2012 | 22755384 |
| widespread tuberculosis including renal involvement. | renal and urogenital disease is a prevalent finding of extrapulmonary mycobacterium tuberculosis. patients can present with unusual complaints not immediately suspicious for tuberculosis. we describe a 38-year-old man who presented with vomiting and an acute kidney injury. imaging studies showed nodules throughout the lungs, retroperitoneum, abdominal viscera, and kidneys. asymmetrical hydronephrosis was found on renal imaging. a classic beaded ureteral appearance (ureteritis cystica) was found ... | 2012 | 22754123 |
| sublineages of beijing strain of mycobacterium tuberculosis in sri lanka. | strains of the beijing/w genotype of mycobacterium tuberculosis have been responsible for large outbreaks of tuberculosis around the world, sometimes involving multi-drug resistance. it has been shown that more recently evolved beijing sublineages are prone to cause outbreaks. furthermore beijing is the single predominant cluster in sri lanka. the present study identifies that recently evolved sublineages of beijing strains are present in the study population. the majority of beijing isolates (9 ... | 2011 | 22754025 |
| rapid molecular diagnosis of pulmonary tuberculosis in children using nasopharyngeal specimens. | a rapid diagnosis of pediatric pulmonary tuberculosis (ptb) using xpert mtb/rif (mycobacterium tuberculosis/rifampicin) automated testing on induced sputum (is) is possible, but the capacity for performing is is limited. the diagnosis using a nasopharyngeal aspirate (npa), which can be non-invasively obtained, is desirable. | 2012 | 22752518 |
| cross-docking study on inha inhibitors: a combination of autodock vina and pm6-dh2 simulations to retrieve bio-active conformations. | inha, the nadh-dependent enoyl-acyl carrier protein reductase from mycobacterium tuberculosis (mtb) is the proposed main target of the first-line antituberculosis drug isoniazid (inh). inh activity is dependent on activation by the catalase peroxidase katg, a mtb enzyme whose mutations are linked to clinical resistance to inh. other inhibitors of inha that do not require any preliminary activation are known. the design of such direct potent inhibitors represents a promising approach to circumven ... | 2012 | 22751934 |
| crystallization and preliminary x-ray diffraction analysis of the high molecular weight ketoacyl reductase fabg4 complexed with nadh. | fabg4 from mycobacterium tuberculosis belongs to the high molecular weight ketoacyl reductases (hmwfabgs). the enzyme requires nadh for β-ketoacyl reductase activity. the protein was overexpressed, purified to homogeneity and crystallized as a fabg4-nadh complex. a mountable fabg4:nadh complex crystal diffracted to 2.59 å resolution and belonged to space group p1, with unit-cell parameters a = 63.07, b = 71.03, c = 92.92 å, α = 105.02, β = 97.06, γ = 93.66°. the matthews coefficient suggested th ... | 2012 | 22750865 |
| mycobacterium tuberculosis manipulates pulmonary apcs subverting early immune responses. | alveolar macrophages (am) and dendritic cells (dcs) are the main antigen presenting cells (apcs) in the respiratory tract. whereas macrophages have been extensively studied in tuberculosis, in situ interactions of dc with mycobacterium tuberculosis (mtb) are poorly explored. we aimed to characterize lung apcs during pulmonary tuberculosis in balb/c mice infected with mtb h37rv. mtb-infection via the airways induced a delayed and continuous accumulation of dcs and am in the lungs. while lung dcs ... | 2013 | 22749983 |
| interferon-γ, tumor necrosis factor, and interleukin-18 cooperate to control growth of mycobacterium tuberculosis in human macrophages. | mycobacterium tuberculosis (mtb) remains a leading infectious threat to human health. macrophages are the cells targeted for infection by the bacterium as well as key effector cells for clearance of the pathogen. interleukin (il)-27 opposes macrophage-mediated control of mtb because supplying il-12 and blocking the activity of il-27 limits bacterial growth in primary human macrophages. the purpose of this study was to determine the immunological regulators of this macrophage mechanism to restric ... | 2012 | 22749533 |
| relationship between 25-hydroxyvitamin d levels and treatment course of pulmonary tuberculosis. | low serum vitamin d level is associated with a high risk of developing active tuberculosis (tb). we investigated the relationships between serum vitamin d levels and clinical course of tb after standard chemotherapy in hospitalized non-hiv patients with tb. | 2012 | 22749249 |
| activity of iqg-607, a new orally active compound, in a murine model of mycobacterium tuberculosis infection. | we have previously demonstrated a potent in vitro inhibitory activity for two pentacyano(isoniazid)ferrate(ii) compounds, namely iqg-607 and iqg-639, against the mycobacterium tuberculosis enoyl-acyl carrier protein reductase enzyme. in this study, the activity of these compounds was evaluated using an in vivo murine model of tuberculosis. swiss mice were infected with m. tuberculosis h37rv strain and then iqg-607 or iqg-639 (250 mg/kg) was administered for 28 days or 56 days. in addition, a dos ... | 2012 | 22748570 |
| rapid detection of rifampicin, isoniazid and streptomycin resistance in mycobacterium tuberculosis clinical isolates by high-resolution melting curve analysis. | this study was carried out to evaluate high-resolution melting (hrm) curve analysis assay for detection of mutations in three drug resistance-associated genes of mycobacterium tuberculosis. | 2012 | 22747769 |
| nitrate reductase assay using sodium nitrate for rapid detection of multidrug resistant tuberculosis. | we validated the nitrate reductase assay (nra) for the detection of multidrug-resistant mycobacterium tuberculosis (mdr-tb) using sodium nitrate (nano3) in replacement of potassium nitrate (kno3) as nitrate source. nano3 is cheaper than kno3 and has no restriction on use which facilitates the implementation of nra to detect mdr-tb. | 2012 | 24031916 |
| tuberculosis trends in saudis and non-saudis in the kingdom of saudi arabia--a 10 year retrospective study (2000-2009). | tuberculosis (tb) remains a public health problem in the kingdom of saudi arabia (ksa), which has a very large labour force from high tb endemic countries. understanding the epidemiological and clinical features of the tb problem, and the tb burden in the immigrant workforce, is necessary for improved planning and implementation of tb services and prevention measures. | 2012 | 22745765 |
| wild-type phosphoribosylpyrophosphate synthase (prs) from mycobacterium tuberculosis: a bacterial class ii prs? | the 5-phospho-α-d-ribose 1-diphosphate (prpp) metabolite plays essential roles in several biosynthetic pathways, including histidine, tryptophan, nucleotides, and, in mycobacteria, cell wall precursors. prpp is synthesized from α-d-ribose 5-phosphate (r5p) and atp by the mycobacterium tuberculosis prsa gene product, phosphoribosylpyrophosphate synthase (mtprs). here, we report amplification, cloning, expression and purification of wild-type mtprs. glutaraldehyde cross-linking results suggest tha ... | 2012 | 22745722 |
| tuberculous pericarditis presenting as massive haemorrhagic pericardial effusion. | tuberculous pericarditis is an exceedingly rare but a well-described extra-pulmonary manifestation of tuberculosis (tb) infection in hong kong. an 82-year-old woman with a known history of diabetes mellitus, hypertension and hyperlipidaemia was admitted for congestive heart failure. routine echocardiographic study during admission revealed a massive pericardial effusion (~4 cm in thickness) but with no tamponade effect. pericardiocentesis was performed and 1.6 l of heavily blood stained fluid wa ... | 2012 | 22744246 |