| antimicrobial, biofilm inhibitory and anti-infective activity of metallic nanoparticles against pathogens mrsa and pseudomonas aeruginosa pa01. | the emergence of drug resistant pathogens is a major concern to the scientific community. novel approaches such as the use of functionalized nanomaterials with antimicrobial activity is required to treat infectious diseases. | 2017 | 28440203 |
| myriocin treatment of cf lung infection and inflammation: complex analyses for enigmatic lipids. | our aim was to use quantitative and qualitative analyses to gain further insight into the role of ceramide in cystic fibrosis (cf). sphingolipid ceramide is a known inflammatory mediator, and its accumulation in inflamed lung has been reported in different types of emphysema, chronic obstructive pulmonary disease and cf. cf is caused by a mutation of the chloride channel and associated with hyperinflammation of the respiratory airways and high susceptibility to ongoing infections. we have previo ... | 2017 | 28439630 |
| cas1 and the csy complex are opposing regulators of cas2/3 nuclease activity. | the type i-f crispr adaptive immune system in pseudomonas aeruginosa (pa14) consists of two crispr loci and six crispr-associated (cas) genes. type i-f systems rely on a crispr rna (crrna)-guided surveillance complex (csy complex) to bind foreign dna and recruit a trans-acting nuclease (i.e., cas2/3) for target degradation. in most type i systems, cas2 and cas3 are separate proteins involved in adaptation and interference, respectively. however, in i-f systems, these proteins are fused into a si ... | 2017 | 28438998 |
| role of psl genes in antibiotic tolerance of adherent pseudomonas aeruginosa. | bacteria attached to a surface are generally more tolerant to antibiotics than their planktonic counterparts, even without the formation of a biofilm. the mechanism of antibiotic tolerance in biofilm communities is multifactorial, and the genetic background underlying this antibiotic tolerance has not yet been fully elucidated. using transposon mutagenesis, we isolated a mutant with reduced tolerance to biapenem (relative to that of the wild type) from adherent cells. sequencing analysis reveale ... | 2017 | 28438927 |
| catalytic reduction of 4-nitrophenol and photo inhibition of pseudomonas aeruginosa using gold nanoparticles as photocatalyst. | a simple, green method is described for the synthesis of gold (au) nanoparticles (nps) using cotoneaster horizontalis extract as a phyto-reducer and capping agent with superior photo inhibition activity against pseudomonas aeruginosa. different from the other methods used elevated temperatures for nanoparticles synthesis, the novelty of our method lies in its energy saving process and fast synthesis rates (~5min for aunps), and its potential to tune the nanoparticles size and afterward their cat ... | 2017 | 28437746 |
| effects of diagnosis by newborn screening for cystic fibrosis on weight and length in the first year of life. | since the implementation of universal newborn screening (nbs) for cystic fibrosis (cf), the timing and magnitude of growth deficiency or its association with correlates of disease among infants with cf who underwent nbs has not been well described. | 2017 | 28437538 |
| risk factors for mortality before age 18 years in cystic fibrosis. | understanding early-life risk factors for childhood death in cystic fibrosis (cf) is important for clinical care, including the identification of effective interventions. | 2017 | 28436621 |
| antibacterial activity of desert truffles from saudi arabia against staphylococcus aureus and pseudomonas aeruginosa. | medicinal mushrooms represent an unlimited source of polysaccharides with nutritional, antitumoral, antibacterial, and immune-stimulating properties. traditional studies of epigeous higher basidiomycetes have recently been joined by studies of hypogeous fungi and, in particular, of so-called desert truffles. with the aim to obtain novel agents against bacteria of clinical importance, we focused on the edible desert truffle mushrooms tirmania pinoyi, terfezia claveryi, and picoa juniperi as sourc ... | 2017 | 28436320 |
| mechanisms responsible for imipenem resistance among pseudomonas aeruginosa clinical isolates exposed to imipenem concentrations within the mutant selection window. | the aim of this study was to determine the propensities of imipenem to select for resistant pseudomonas aeruginosa mutants by determining the mutant prevention concentrations (mpcs) for 9 unrelated clinical isolates and the accession of any relationship with mechanisms of resistance development. the mpc/mic ratios ranged from 4 to 16. detection of resistance mechanisms in the mutant derivatives of the nine isolates mainly revealed inactivating mutations in the gene coding for outer membrane prot ... | 2017 | 28434899 |
| plackett-burman design and response surface optimization of medium trace nutrients for glycolipopeptide biosurfactant production | a glycolipopeptide biosurfactant produced by pseudomonas aeruginosa strain ikw1 reduced the surface tension of fermentation broth from 71.31 to 24.62 dynes/cm at a critical micelle concentration of 20.80 mg/l. the compound proved suitable for applications in emulsion stabilization in food, as well as in cosmetic and pharmaceutical formulations. | 2017 | 28433004 |
| intratracheal instillation of neutrophils rescues bacterial overgrowth initiated by trauma damage-associated molecular patterns. | nosocomial pneumonias are common in trauma patients and so interventions to prevent and treat nosocomial pneumonia may improve outcomes. our prior work strongly suggests that tissue injury predisposes to infections like nosocomial pneumonia because mitochondrial debris originating from injured cells contains damage-associated molecular patterns that can reduce neutrophil (pmn) migration into the airway and diminish pmn function in response to bacterial inoculation of the airway. this suggested t ... | 2017 | 28431414 |
| potentiation of antibiotic against psedomonas aeruginosa biofilm: a study with plumbagin and gentamicin. | pseudomonas aeruginosa is one of the fatal biofilm forming pathogens which pose to be a problem in clinical infections, contamination of food and marine ecosystems. in this report a napthoquinone - plumbagin has been explored for its antimicrobial (antibacterial and antibiofilm) activity against p. aeruginosa biofilm. the ability of plumbagin to enhance the bioactivity of a known broad spectrum antibiotic was further assayed by combining the sub-mic doses of plumbagin with sub-mic doses of genta ... | 2017 | 28429871 |
| draft genome sequence of a multidrug-resistant pseudomonas aeruginosa strain isolated from a patient with a urinary tract infection in khartoum, sudan. | pseudomonas aeruginosa infection is difficult to treat due to the presence of antibiotic resistance determinants. here, we report the genome sequence of a multidrug-resistant p. aeruginosa strain isolated from a patient with a urinary tract infection in 2015. | 2017 | 28428302 |
| the pseudomonas aeruginosa lectin leca triggers host cell signalling by glycosphingolipid-dependent phosphorylation of the adaptor protein crkii. | the human pathogen pseudomonas aeruginosa induces phosphorylation of the adaptor protein crkii by activating the non-receptor tyrosine kinase abl to promote its uptake into host cells. so far, specific factors of p. aeruginosa, which induce abl/crkii signalling, are entirely unknown. in this research, we employed human lung epithelial cells h1299, chinese hamster ovary cells and p. aeruginosa wild type strain pao1 to study the invasion process of p. aeruginosa into host cells by using microbiolo ... | 2017 | 28428058 |
| glucosylceramide critically contributes to the host defense of cystic fibrosis lungs. | cystic fibrosis (cf) is the most common autosomal-recessive disorder in western countries. previous studies have demonstrated an important role of sphingolipids in the pathophysiology of cystic fibrosis. it has been shown that ceramide has a central role in various pulmonary infections, including those with pseudomonas aeruginosa (p. aeruginosa). ceramide is accumulated in the airways of cf mice and patients. however, little is known about a potential role of glucosylceramide in cystic fibrosis. | 2017 | 28427052 |
| correction: modulation of behaviour and virulence of a high alginate expressing pseudomonas aeruginosa strain from cystic fibrosis by oral commensal bacterium streptococcus anginosus. | [this corrects the article doi: 10.1371/journal.pone.0173741.]. | 2017 | 28426833 |
| her2-specific recombinant immunotoxin 4d5scfv-pe40 passes through retrograde trafficking route and forces cells to enter apoptosis. | immunotoxin 4d5scfv-pe40 is a recombinant protein that comprises 4d5scfv antibody as a targeting module and fragment of pseudomonas exotoxin a as an effector (toxic) one. the immunotoxin has shown pronounced antitumor effect on cancer cells overexpressing her2 receptor in vitro and on her2-positive experimental tumors in vivo. we clarified the mechanism of 4d5scfv-pe40 activity that is of particular importance in the case of targeted therapeutic agent aimed at personalizing treatment of disease ... | 2017 | 28423549 |
| resolvin d1 enhances the resolution of lung inflammation caused by long-term pseudomonas aeruginosa infection. | pseudomonas aeruginosa lung infection is a main cause of disability and mortality worldwide. acute inflammation and its timely resolution are crucial for ensuring bacterial clearance and limiting tissue damage. here, we investigated protective actions of resolvin (rv) d1 in lung infection induced by the rp73 clinical strain of p. aeruginosa. rvd1 significantly diminished bacterial growth and neutrophil infiltration during acute pneumonia caused by rp73. inoculum of rp73, immobilized in agar bead ... | 2017 | 28422188 |
| prevention of bacterial biofilm formation on soft contact lenses using natural compounds. | in eye care field, contact lenses (cl) have a great impact on improving vision, but their use can be limited by ocular infection. cl- associated infections can be reduced by good attention to cl storage case practice. cl-care solutions should be able to control microbial growth on cl. the aim of the study was to evaluate and compare the efficacy of cl-care solutions (found in egyptian market) with some natural compounds in removal and inhibition of bacterial biofilm formed on soft cl. clinical i ... | 2017 | 28421562 |
| in vivo and in vitro interactions between pseudomonas aeruginosa and staphylococcus spp. | the significance of polymicrobial infections is increasingly being recognized especially in a biofilm context wherein multiple bacterial species-including both potential pathogens and members of the commensal flora-communicate, cooperate, and compete with each other. two important bacterial pathogens that have developed a complex network of evasion, counter-inhibition, and subjugation in their battle for space and nutrients are pseudomonas aeruginosa and staphylococcus aureus. their strain- and ... | 2017 | 28421166 |
| transcriptomic and metabolomics profiling of phage-host interactions between phage pap1 and pseudomonas aeruginosa. | the basic biology of bacteriophage-host interactions has attracted increasing attention due to a renewed interest in the therapeutic potential of bacteriophages. in addition, knowledge of the host pathways inhibited by phage may provide clues to novel drug targets. however, the effect of phage on bacterial gene expression and metabolism is still poorly understood. in this study, we tracked phage-host interactions by combining transcriptomic and metabolomic analyses in pseudomonas aeruginosa infe ... | 2017 | 28421049 |
| two arginine residues in the cooh-terminal of human β-defensin-3 constitute an essential motif for antimicrobial activity and il-6 production. | human β-defensin-3 (hbd-3) possesses antimicrobial activities and the potential to induce proinflammatory cytokines. hbd-3 contains a unique motif of two arginine residues (arg or r) in the cooh-terminal region. to understand the bioactive properties of the arg residues of hbd-3, we examined antimicrobial activities against staphylococcus aureus and pseudomonas aeruginosa using synthetic hbd-2, hbd-3 and two variant peptides of hbd-3: the arg-truncated variant designated desr hbd-3 and nrr hbd-3 ... | 2017 | 28418601 |
| expression of toll-like receptors in t lymphocytes stimulated with n-(3-oxododecanoyl)-l-homoserine lactone from pseudomonas aeruginosa. | the establishment of chronic pseudomonas aeruginosa infections is correlated with the disturbance of the host immune system. the p. aeruginosa quorum-sensing molecule n-3-(oxododecanoyl)-l-homoserine lactone (3-o-c12-hsl) has the potential to modulate the host immune system. the immune system recognizes pathogens via toll-like receptors (tlrs). we found that 3-o-c12-hsl induced tlr changes in monocytes. however, the role of t cells in p. aeruginosa infection has not been delineated. in order to ... | 2017 | 28418096 |
| assessment of biofilm removal capacity of a broad host range bacteriophage jhp against pseudomonas aeruginosa. | pseudomonas aeruginosa is an efficient biofilm-dwelling microbial pathogen, associated with nosocomial infections. these biofilm-associated infections are resistant to antibiotics and immune defenses, therefore pose major problem against their treatment. this scenario demands alternative therapeutic regimens, and bacteriophage therapy is one among potential strategies for clinical management of multiple drug resistance. in this investigation, the efficacy of a bacteriophage, jhp, is evaluated to ... | 2017 | 28418081 |
| degradation of staphylococcal enterotoxin a by a pseudomonas aeruginosa isolate from raw milk. | recently, we found that staphylococcal enterotoxin a (sea)-producing staphylococcus aureus strains produced sea in raw milk with microbial contaminants at high temperatures like 40 °c only. moreover, the concentration of sea produced in raw milk gradually decreased after the peak. the reason(s) for sea degradation in raw milk was studied in this study. degradation of sea spiked in raw milk was observed at 40 °c, but not at 25 °c. a pseudomonas aeruginosa isolate from raw milk degraded sea spiked ... | 2017 | 28417705 |
| prophylactic anti-staphylococcal antibiotics for cystic fibrosis. | staphylococcus aureus causes pulmonary infection in young children with cystic fibrosis. prophylactic antibiotics are prescribed hoping to prevent such infection and lung damage. antibiotics have adverse effects and long-term use might lead to infection with pseudomonas aeruginosa. this is an update of a previously published review. | 2017 | 28417451 |
| pharmacokinetics and dialytic clearance of ceftazidime-avibactam in a critically ill patient on continuous venovenous hemofiltration. | ceftazidime-avibactam administered at 1.25 g every 8 h was used to treat multidrug-resistant pseudomonas aeruginosa bacteremia in a critically ill patient on continuous venovenous hemofiltration (cvvh). prefiltration plasma drug concentrations of ceftazidime and avibactam were measured at 0, 1, 2, 4, 6, and 8 h along with postfiltration and ultrafiltrate concentrations at h 2 and h 6. plasma pharmacokinetic parameters of ceftazidime and avibactam, respectively, were as follows: maximum plasma co ... | 2017 | 28416553 |
| pseudomonas aeruginosa eradication: finally moving the needle? | | 2017 | 28416146 |
| β-catenin promotes intracellular bacterial killing via suppression of pseudomonas aeruginosa-triggered macrophage autophagy. | objective to investigate β-catenin-mediated bacterial elimination during pseudomonas aeruginosa infection of macrophage-like raw264.7 cells. methods cell viability and catenin beta 1 ( ctnnb1) expression in raw264.7 cells following p. aeruginosa infection versus uninfected cells, were detected by cell counting kit-8 assay and β-catenin western blots. raw264.7 cells with ctnnb1 overexpression were established with β-catenin lentivirus using flow cytometry and clonogenic limiting dilution assays. ... | 2017 | 28415949 |
| pseudomonas aeruginosa endophthalmitis caused by accidental iatrogenic ocular injury with a hypodermic needle. | iatrogenic traumatic endophthalmitis is a rare but serious ocular infection that can lead to severe vision loss. a 44-year-old man presented with pain and decreased vision in the right eye 4 hours after injury with a hypodermic needle during irrigation of his eye. slit-lamp examination revealed a penetrating corneal puncture and iris hole in the right eye. twenty hours later, his visual acuity had decreased to hand motion, and severe fibrinoid uveitis was noted. he immediately underwent irrigati ... | 2017 | 28415945 |
| fabrication of polypropylene/silver nanocomposites for biocidal applications. | this paper presents a study on biocidal effect of polymer nanocomposite films of gamma irradiated polypropylene (pp) and silver nanoparticles. the modified polypropylene was obtained from isotactic polypropylene (ipp) in pellets form by irradiation with gamma rays in the presence of acetylene. a new morphology with long chain branching of pp and distinct rheology is obtained by this process. the blend of 50/50wt% neat pp and pp modified by gamma radiation were further mixed using a twin screw ex ... | 2017 | 28415538 |
| immobilization and stabilization of acylase on carboxylated polyaniline nanofibers for highly effective antifouling application via quorum quenching. | acylase (ac) was immobilized and stabilized on carboxylated polyaniline nanofibers (cpanfs) for the development of antifouling nanobiocatalysts with high enzyme loading and stability. ac was immobilized via three different approaches: covalent attachment (ca), enzyme coating (ec), and magnetically separable enzyme precipitate coating (mag-epc). the enzyme activity per unit weight of cpanfs with mag-epc was 75 and 300 times higher than that of those with ca and ec, respectively, representing impr ... | 2017 | 28414213 |
| inhibition of the mitochondrial respiratory chain in gills of rhamdia quelen experimentally infected by pseudomonas aeruginosa: interplay with reactive oxygen species. | it has long been recognized that there are several infectious diseases linked to the impairment of enzymatic complexes of the mitochondrial respiratory chain, with consequent production of reactive oxygen species (ros), that contribute to disease pathogenesis. in this study, we determined whether the inhibition on mitochondrial respiratory chain might be considered a pathway involved in the production of ros in gills of rhamdia quelen experimentally infected by p. aeruginosa. the animals were di ... | 2017 | 28414167 |
| crystal structure of pseudomonas aeruginosa n-acetyltransferase pa4534. | the gcn5-related n-acetyltransferase (gnat) superfamily includes a large and diverse group of enzymes that catalyzes the transfer of an acetyl group from acetyl coenzyme a (ac-coa) to the amine group of a substrate. substrates include protein n-terminus, lysine of histone tails, and other small molecules such as aminoglycoside, serotonin, and glucose-6-phosphate. gnat superfamily of proteins is involved in many physiologically important reactions in eukaryotes and prokaryotes. however, functions ... | 2017 | 28412357 |
| use of ceftolozane/tazobactam as salvage therapy for infections due to extensively drug-resistant pseudomonas aeruginosa. | | 2017 | 28412272 |
| photodynamic therapy with methylene blue for skin ulcers infected with pseudomonas aeruginosa and fusarium spp. | photodynamic therapy (pdt) is a therapeutic modality with significant antimicrobial activity. we present 2 cases of chronic lower limb ulcers in which fungal and bacterial superinfection complicated management. pdt with methylene blue as the photosensitizer led to clinical and microbiological cure with no significant adverse effects. pdt with methylene blue is a valid option for the management of superinfected chronic ulcers, reducing the use of antibiotics and the induction of resistance. | 2017 | 28411862 |
| monoclonal antibodies to the reactive centre loop (rcl) of human corticosteroid-binding globulin (cbg) can protect against proteolytic cleavage. | corticosteroid-binding globulin (cbg) binds most of the cortisol in circulation and is a non-functional member of the family of serine protease inhibitors (serpins) with an exposed elastase sensitive reactive centre loop (rcl). the rcl can be cleaved by human neutrophil elastase, released from activated neutrophils, and can also be cleaved at nearby site(s) by elastase released by pseudomonas aeruginosa, and at two further sites, also within the rcl, by bovine chymotrypsin. cleavage of the rcl r ... | 2017 | 28411181 |
| enhancing enzymatic hydrolysis of coconut husk through pseudomonas aeruginosa ap 029/glviia rhamnolipid preparation. | this work investigated the influence of chemical (triton x-100) and biological surfactant preparation (rhamnolipids) in coconut husk hydrolysis that was subjected to pretreatment with acid-alkali or alkaline hydrogen peroxide. the natural and pretreated biomass was characterized using the national renewable energy laboratory protocol analysis as well as x-ray diffraction and scanning electron microscopy. the results demonstrated that in terms of the total reducing sugars, there was no significan ... | 2017 | 28411051 |
| amphiphilic tobramycin-lysine conjugates sensitize multidrug resistant gram-negative bacteria to rifampicin and minocycline. | chromosomally encoded low membrane permeability and highly efficient efflux systems are major mechanisms by which pseudomonas aeruginosa evades antibiotic actions. our previous reports have shown that amphiphilic tobramycin-fluoroquinolone hybrids can enhance efficacy of fluoroquinolone antibiotics against multidrug-resistant (mdr) p. aeruginosa isolates. herein, we report on a novel class of tobramycin-lysine conjugates containing an optimized amphiphilic tobramycin-c12 tether that sensitize gr ... | 2017 | 28409644 |
| hypoxia and the hypoxia-regulated transcription factor hif-1α suppress the host defence of airway epithelial cells. | chronic diseases of the respiratory tract, such as cystic fibrosis, are associated with mucosal and systemic hypoxia. innate immune functions of airway epithelial cells are required to prevent and control infections of the lung parenchyma. the transcription factor hypoxia-inducible factor 1α (hif-1α) regulates cellular adaptation to low oxygen conditions. here, we show that hypoxia and hif-1α regulate innate immune mechanisms of cultured human bronchial epithelial cells (hbecs). exposure of prim ... | 2017 | 28409544 |
| nuclear protein hmgn2 attenuates pyocyanin-induced oxidative stress via nrf2 signaling and inhibits pseudomonas aeruginosa internalization in a549 cells. | pyocyanin (pcn, 1-hydroxy-5-methyl-phenazine) is one of the most essential virulence factors of pseudomonas aeruginosa (pa) to cause various cytotoxic effects in long-term lung infectious diseases, however the early effect of this bacterial toxin during pa infection and subsequent autonomous immune response in host cells have not been fully understood yet. our results display that early onset of pcn stimulates pseudomonas aeruginosa pao1 adhesion and invasion in a549 cells via ros production. no ... | 2017 | 28408162 |
| microenvironmental characteristics and physiology of biofilms in chronic infections of cf patients are strongly affected by the host immune response. | in vitro studies of pseudomonas aeruginosa and other pathogenic bacteria in biofilm aggregates have yielded detailed insight into their potential growth modes and metabolic flexibility under exposure to gradients of substrate and electron acceptor. however, the growth pattern of p. aeruginosa in chronic lung infections of cystic fibrosis (cf) patients is very different from what is observed in vitro, for example, in biofilms grown in flow chambers. dense in vitro biofilms of p. aeruginosa exhibi ... | 2017 | 28407427 |
| a short history of microbial biofilms and biofilm infections. | the observation of aggregated microbes surrounded by a self-produced matrix adhering to surfaces or located in tissues or secretions is old since both leeuwenhoek and pasteur have described the phenomenon. in environmental and technical microbiology, biofilms, 80-90 years ago, were already shown to be important for biofouling on submerged surfaces, for example, ships. the concept of biofilm infections and their importance in medicine was, however, initiated in the early 1970s by the observation ... | 2017 | 28407426 |
| environmental modification via a quorum sensing molecule influences the social landscape of siderophore production. | bacteria produce a wide variety of exoproducts that favourably modify their environment and increase their fitness. these are often termed 'public goods' because they are costly for individuals to produce and can be exploited by non-producers (cheats). the outcome of conflict over public goods is dependent upon the prevailing environment and the phenotype of the individuals in competition. many bacterial species use quorum sensing (qs) signalling molecules to regulate the production of public go ... | 2017 | 28404780 |
| efficacy of pseudomonas aeruginosa eradication regimens in bronchiectasis. | | 2017 | 28404646 |
| preparation of pseudomonas aeruginosa alginate-flagellin immunoconjugate. | mucoid strains of pseudomonas aeruginosa are closely associated with chronic pulmonary infections. in this report we describe a straightforward approach to conjugate high molecular weight alginate to type b-flagellin (flb) and investigation of its bioactivity. the conjugation process was performed by using adh and edac. the endotoxin was eliminated from the candidate vaccine by lps removal resin followed by lal test. the bioconjugate molecules were verified by simultaneously determination of pol ... | 2017 | 28404459 |
| lung structure and function similarities between primary ciliary dyskinesia and mild cystic fibrosis: a pilot study. | primary ciliary dyskinesia (pcd) and cystic fibrosis (cf) are increasingly compared. there are no chest magnetic resonance imaging (mri) comparative studies of pcd and cf. we assessed clinical, functional, microbiological and mri findings in pcd and mild cf patients in order to evaluate different expression of lung disease. | 2017 | 28403885 |
| influence of physical effects on the swarming motility of pseudomonas aeruginosa. | many species of bacteria can spread over a moist surface via a particular form of collective motion known as "surface swarming". this form of motility is typically studied by inoculating bacteria on a gel formed by 0.4-1.5% agar, which contains essential nutrients for their growth and proliferation. using pseudomonas aeruginosa and its pili-less mutant, δpila, we investigate physical factors that either facilitate or restrict the swarming motility, measured by the rate of increase in area covere ... | 2017 | 28402888 |
| simultaneous and quantitative monitoring of co-cultured pseudomonas aeruginosa and staphylococcus aureus with antibiotics on a diffusometric platform. | successful treatments against bacterial infections depend on antimicrobial susceptibility testing (ast). however, conventional ast requires more than 24 h to obtain an outcome, thereby contributing to high patient mortality. an antibiotic therapy based on experiences is therefore necessary for saving lives and escalating the emergence of multidrug-resistant pathogens. accordingly, a fast and effective drug screen is necessary for the appropriate administration of antibiotics. the mixed pathogeni ... | 2017 | 28402317 |
| a tobramycin vector enhances synergy and efficacy of efflux pump inhibitors against multidrug-resistant gram-negative bacteria. | drug efflux mechanisms interact synergistically with the outer membrane permeability barrier of gram-negative bacteria, leading to intrinsic resistance that presents a major challenge for antibiotic drug development. efflux pump inhibitors (epis) which block the efflux of antibiotics synergize antibiotics, but the clinical development of epi/antibiotic combination therapy to treat multidrug-resistant (mdr) gram-negative infections has been challenging. this is in part caused by the inefficiency ... | 2017 | 28399372 |
| pseudomonas aeruginosa liver abscess as the first manifestation of x-linked agammaglobulinemia with a novel mutation. | | 2017 | 28398200 |
| second harmonic generation imaging of corneal stroma after infection by pseudomonas aeruginosa. | pseudomonas aeruginosa is a pathogenic gram-negative organism that has the ability to cause blinding corneal infections following trauma and during contact lens wear. in this study, we investigated the directional movement and orientation of an invasive corneal isolate of p. aeruginosa in the corneal stroma during infection of ex vivo and in vivo rabbit corneas using multiphoton fluorescence and second harmonic generation (shg) imaging. ex vivo, rabbit corneas were subject to three partial thick ... | 2017 | 28397809 |
| in vitro studies on a microfluidic sensor with embedded obstacles using new antibacterial synthetic compounds (1-tdppo) mixed prop-2-en-1-one with difluoro phenyl. | this paper describes the use of an analytical microfluidic sensor for accelerating chemo-repellent response and strong anti-bacterial 1-(thien-2-yl)-3-(2, 6-difluoro phenyl) prop-2-en-1-one (1-tdppo). the chemically-synthesized antimicrobial agent, which included prop-2-en-1-one and difluoro phenyl groups, was moving through an optically transparent polydimethylsiloxane (pdms) microfluidic sensor with circular obstacles arranged evenly. the response, growth and distribution of fluorescent labeli ... | 2017 | 28397751 |
| factors associated with infectious laryngitis: a retrospective review of 15 cases. | to identify the culturable microbes associated with infectious laryngitis and outline effective treatment strategies. | 2017 | 28397557 |
| multi-metal resistance and plant growth promotion potential of a wastewater bacterium pseudomonas aeruginosa and its synergistic benefits. | water and soil pollution by toxic heavy metals (hms) is increasing globally because of increase in population, industrialization and urbanization. it is a burning problem for the public, scientists, academicians and politicians how to tackle the toxic contaminants which jeopardize the environment. one possible solution for pollution abatement is a bioremediation-effective and innovative technology that uses biological systems for treatment of contaminants. many bacteria synthesize indole-3-aceti ... | 2017 | 28397062 |
| bacterial secretant from pseudomonas aeruginosa dampens inflammasome activation in a quorum sensing-dependent manner. | inflammasome signaling can contribute to host innate immune defense against bacterial pathogens such as pseudomonas aeruginosa. however, bacterial evasion of host inflammasome activation is still poorly elucidated. quorum sensing (qs) is a bacterial communication mechanism that promotes coordinated adaptation by triggering expression of a wide range of genes. qs is thought to strongly contribute to the virulence of p. aeruginosa, but the molecular impact of bacterial qs on host inflammasome defe ... | 2017 | 28396663 |
| salicylidene acylhydrazides and hydroxyquinolines act as inhibitors of type three secretion systems in pseudomonas aeruginosa by distinct mechanisms. | type 3 secretion systems (t3sss) are major virulence factors in gram-negative bacteria. pseudomonas aeruginosa expresses two t3sss, namely, an injectisome (it3ss) translocating effector proteins in the host cell cytosol and a flagellum (ft3ss) ensuring bacterial motility. inhibiting these systems is an appealing therapeutic strategy for acute infections. this study examines the protective effects of the salicylidene acylhydrazide inp0341 and of the hydroxyquinoline inp1750 (previously described ... | 2017 | 28396545 |
| structural basis for lipopolysaccharide extraction by abc transporter lptb2fg. | after biosynthesis, bacterial lipopolysaccharides (lps) are transiently anchored to the outer leaflet of the inner membrane (im). the atp-binding cassette (abc) transporter lptb2fg extracts lps molecules from the im and transports them to the outer membrane. here we report the crystal structure of nucleotide-free lptb2fg from pseudomonas aeruginosa. the structure reveals that lipopolysaccharide transport proteins lptf and lptg each contain a transmembrane domain (tmd), a periplasmic β-jellyroll- ... | 2017 | 28394325 |
| emergence of complex behavior in pili-based motility in early stages of p. aeruginosa surface adaptation. | pseudomonas aeruginosa move across surfaces by using multiple type iv pili (tfp), motorized appendages capable of force generation via linear extension/retraction cycles, to generate surface motions collectively known as twitching motility. pseudomonas cells arrive at a surface with low levels of piliation and tfp activity, which both progressively increase as the cells sense the presence of a surface. at present, it is not clear how twitching motility emerges from these initial minimal conditio ... | 2017 | 28393835 |
| emergence of colistin resistance in pseudomonas aeruginosa st235 clone in south korea. | in this study, the prevalence and characteristics of metallo-β-lactamase (mbl)-producing pseudomonas aeruginosa isolates in south korea were investigated. among 215 p. aeruginosa isolates collected from eight hospitals, 77 (35.8%) and 72 (33.5%) were resistant to imipenem and meropenem, respectively. of the 77 imipenem-resistant isolates, mbl genes were identified in 34 isolates (blaimp-6 in 33 isolates and blavim-2 in 1 isolate). all of the mbl-producing isolates belonged to a globally prevaili ... | 2017 | 28392440 |
| active-site flexibility and substrate specificity in a bacterial virulence factor: crystallographic snapshots of an epoxide hydrolase. | pseudomonas aeruginosa secretes an epoxide hydrolase with catalytic activity that triggers degradation of the cystic fibrosis transmembrane conductance regulator (cftr) and perturbs other host defense networks. targets of this cftr inhibitory factor (cif) are largely unknown, but include an epoxy-fatty acid. in this class of signaling molecules, chirality can be an important determinant of physiological output and potency. here we explore the active-site chemistry of this two-step α/β-hydrolase ... | 2017 | 28392259 |
| population pharmacokinetics and dosing simulations of ceftazidime in critically ill patients receiving sustained low-efficiency dialysis. | to describe the population pks of ceftazidime in critically ill patients receiving sustained low-efficiency dialysis (sled). | 2017 | 28175308 |
| antibacterial-nanocomposite bone filler based on silver nanoparticles and polysaccharides. | injectable bone fillers represent an attractive strategy for the treatment of bone defects. these injectable materials should be biocompatible, capable of supporting cell growth and possibly able to exert antibacterial effects. in this work, nanocomposite microbeads based on alginate, chitlac, hydroxyapatite and silver nanoparticles were prepared and characterized. the dried microbeads displayed a rapid swelling in contact with simulated body fluid and maintained their integrity for more than 30 ... | 2016 | 27860429 |
| tobramycin and bicarbonate synergise to kill planktonic pseudomonas aeruginosa, but antagonise to promote biofilm survival. | increasing antibiotic resistance and the declining rate at which new antibiotics come into use create a need to increase the efficacy of existing antibiotics. the aminoglycoside tobramycin is standard-of-care for many types of pseudomonas aeruginosa infections, including those in the lungs of cystic fibrosis (cf) patients. p. aeruginosa is a nosocomial and opportunistic pathogen that, in planktonic form, causes acute infections and, in biofilm form, causes chronic infections. inhaled bicarbonate ... | 2016 | 28721244 |
| silver nanocoatings for reducing the exogenous microbial colonization of wound dressings. | the aim of this work was to obtain an antimicrobial coating (nanoag) for polyester-nylon wound dressings (wds) for reducing the risk of exogenous wound related infections. the as-prepared nanoag-wds were characterized by xrd (x-ray diffraction), sem (scanning electron microscopy), tem (transmission electron microscopy), saed (selected area electron diffraction) and irm (infrared microscopy). biological characterization consisted of in vitro evaluation of the interaction with fibroblast cell cult ... | 2016 | 28773468 |
| a new pseudomonas quinolone signal (pqs) binding partner: mexg. | the opportunistic pathogen pseudomonas aeruginosa utilises the cell-cell signalling mechanism known as quorum sensing to regulate virulence. p. aeruginosa produces two quinolone-based quorum sensing signalling molecules; the pseudomonas quinolone signal (pqs) and its biosynthetic precursor 2-heptyl-4(1h)-quinolone (hhq). to date, only one receptor (the pqsr protein) has been identified that is capable of binding pqs and hhq. here, we report on the synthesis of pqs and hhq affinity probes for che ... | 2016 | 28660026 |
| rapid assay to assess bacterial adhesion on textiles. | textiles are frequently colonized by microorganisms leading to undesired consequences like hygienic problems. biocidal coatings often raise environmental and health concerns, thus sustainable, biocide-free coatings are of interest. to develop novel anti-adhesive textile coatings, a rapid, reliable, and quantitative high-throughput method to study microbial attachment to fabrics is required, however currently not available. here, a fast and reliable 96-well plate-based screening method is develop ... | 2016 | 28773373 |
| bacteria differently deploy type-iv pili on surfaces to adapt to nutrient availability. | the structure of bacterial biofilms depends on environmental conditions, such as availability of nutrients, during biofilm formation. in turn, variations in biofilm structure in part reflect differences in bacterial motility during early biofilm formation. pseudomonas aeruginosa deprived of nutrients remain dispersed on a surface, whereas cells supplemented with additional nutrients cluster and form microcolonies. at the single-cell scale, how bacteria modify their motility to favour distinct li ... | 2016 | 28721239 |
| co-delivery of nitric oxide and antibiotic using polymeric nanoparticles. | the rise of hospital-acquired infections, also known as nosocomial infections, is a growing concern in intensive healthcare, causing the death of hundreds of thousands of patients and costing billions of dollars worldwide every year. in addition, a decrease in the effectiveness of antibiotics caused by the emergence of drug resistance in pathogens living in biofilm communities poses a significant threat to our health system. the development of new therapeutic agents is urgently needed to overcom ... | 2016 | 28808526 |
| stimulation of rhamnolipid biosurfactants production in pseudomonas aeruginosa ak6u by organosulfur compounds provided as sulfur sources. | a pseudomonas aeruginosa ak6u strain produced rhamnolipid biosurfactants to variable extents when grown on mgso4 or organosulfur compounds as sulfur sources and glucose as a carbon source. organosulfur cultures produced much higher biosurfactants amounts compared to the mgso4 cultures. the surface tension of the growth medium was reduced from 72 mn/m to 54 and 30 mn/m in cultures containing mgso4 and 4,6-dimethyldibenzothiophene (4,6-dm-dbt), respectively. ak6u cultures produced different rhamno ... | 2015 | 28626715 |
| corrigendum to "high-level over-expression, purification, and crystallization of a novel phospholipase c/sphingomyelinase from pseudomonas aeruginosa" [protein expr. purif. 90 (2013) 40-46]. | | 2015 | 28807239 |
| community-acquired pseudomonas aeruginosa-pneumonia in a previously healthy man occupationally exposed to metalworking fluids. | although the pseudomonas aeruginosa infection is well known and frequently found in hospitals and nursing care facilities, many cases are also reported outside these boundaries. in general, this pathogen infects debilitated patients either by comorbidities or by any form of immunodeficiency. in cases of respiratory infection, tobacco abuse seems to play an important role as a risk factor. in previously healthy patients, community-acquired pneumonia (cap) with p. aeruginosa as the etiological age ... | 2017 | 28573116 |
| multi-response optimization of rhamnolipid production using grey rational analysis in taguchi method. | the present paper envisages the multi-response optimization of certain process parameters like total sugars concentration, c/n ratio and incubation time on rhamnolipid yield, surface tension reduction, biomass formation and substrate utilization, in rhamnolipid production by a pseudomonas aeruginosa mutant strain grown on clarified blackstrap molasses, under l9 orthogonal array in taguchi design. the results have been analyzed by using grey relational analysis for the identification of an optimu ... | 2014 | 28626652 |
| antibacterial, anti-glucosidase, and antioxidant activities of selected highland ferns of malaysia. | ferns contain natural products with potential therapeutic applications. current knowledge of the pharmacological properties of ferns, specifically those growing at high altitudes, is limited. this study aimed to evaluate the phytochemical contents as well as antibacterial, anti-glucosidase, and antioxidant activities of four highland ferns in malaysia. | 2013 | 28510890 |
| epidemiological profile and antibiotic resistance of pseudomonas aeruginosa isolates in burn and traumatology center in tunisia over a three-year period. | pseudomonas aeruginosa is a known opportunistic pathogen frequently causing serious infections in burned patients. | 2012 | 28509309 |
| functional identification of the dna packaging terminase from pseudomonas aeruginosa phage pap3. | terminase proteins are responsible for dna recognition and initiation of dna packaging in phages. we previously reported the genomic sequence of a temperate pseudomonas aeruginosa phage, pap3, and determined its precise integration site in the host bacterial chromosome. in this study, we present a detailed functional identification of the dna packaging terminase for phage pap3. the purified large subunit p03 was demonstrated to possess atpase and nuclease activities, as well as the ability to bi ... | 2012 | 23011306 |
| protective efect of brain-derived neurotrophic factor against the ototoxicity of pseudomonas aeruginosa exotoxin a. | objective--the protective effect of brain-derived neurotrophic factor (bdnf) against the ototoxicity resulting from exposure of pseudomonas aeruginosa exotoxin a (paexoa) to the middle ear was analyzed. the combined effect of bdnf and n(g)-nitro-l-arginine methyl ester (l-name) was also investigated. material and methods--six groups of albino rats were instilled through the tympanic membrane into the round window niche with the following solutions: saline; paexoa; bdnf; l-name; paexoa + bdnf; an ... | 2003 | 28425336 |
| evaluation of hospital acquired pseudomonas infection in paraplegic and orthopaedic cases. | one hundred isolates of pseudomonas aeruginosa from patients of the spinal cord injury and orthopaedic centre were evaluated on the basis of 2 laboratory assays; antibiotic sensitivity and pyocin typing. antibiotic drug resistance was found in 54 per cent of the isolates. the urinary tract, pressure sores and wound swabs were the most common clinical specimens. spinal cord injury patients showed a high rate of nosocomial colonisation. ceftazidime, amikacin, norflox and ciprofloxacin were the mos ... | 1998 | 28775405 |
| in vitro comparative activity of eight antipseudomonal agents. | five hundred and thirty strains of pseudomonas aeruginosa isolated from indoor patients were tested against three chemical groups comprising eight antibiotics. high degree of resistant strains ranging from 38.0% to 61.8% were seen against carbenicillin, piperacillin and gentamicin. however amikacin, ciprofloxacin, ceftazidime, netilmicin and norfloxacin showed comparatively better activity with resistant strains ranging from 7.8% to 22.8%. a notably higher rate of resistance was seen in urinary ... | 1994 | 28769199 |
| linker-insertion mutagenesis of pseudomonas aeruginosa outer membrane protein oprf. | the oprf gene, expressing pseudomonas aeruginosa major outer membrane protein oprf, was subjected to semi-random linker mutagenesis by insertion of a 1.3 kb hincll kanamycin-resistance fragment from plasmid puc4kapa into multiple blunt-ended restriction sites in the oprf gene. the kanamycin-resistance gene was then removed by pstl digestion, which left a 12 nucleotide pair linker residue. nine unique clones were identified that contained such linkers at different locations within the oprf gene a ... | 1993 | 28776855 |