| real-time recognition of mycobacterium tuberculosis and lipoarabinomannan using the quartz crystal microbalance. | a quartz crystal microbalance (qcm) immunosensor has been successfully employed to screen for both whole mycobacteria tuberculosis (mtb) bacilli and a mtb surface antigen, lipoarabinomannan (lam). one of the most abundant components of the mtb cell surface, lam, may be detected without the presence of the entire bacterium. using available antibodies with proven utility in enzyme-linked immunoassays (elisas), a sensor was designed to measure mtb bacilli and lam. equilibrium association constants ... | 2012 | 23175600 |
| rapid whole-genome sequencing for investigation of a suspected tuberculosis outbreak. | two southeast asian students attending the same school in the united kingdom presented with pulmonary tuberculosis. an epidemiological investigation failed to link the two cases, and drug resistance profiles of the mycobacterium tuberculosis isolates were discrepant. whole-genome sequencing of the isolates found them to be genetically identical, suggesting a missed transmission event. | 2013 | 23175259 |
| quantiferon-tb gold in-tube test for diagnosis of latent tuberculosis (tb) infection in solid organ transplant candidates: a single-center study in an area endemic for tb. | the prevalence of mycobacterium tuberculosis in transplant recipients is estimated to be 50 times higher than in the general population, with a mortality rate of around 40%. diagnosis and treatment of latent tuberculosis (tb) infection (ltbi) is an essential strategy for tb control. in this study we compared the quantiferon-tb gold in-tube test (qft) with the tuberculin skin test (tst) for detection of ltbi in solid organ transplant (sot) candidates. | 2013 | 23173720 |
| embb306 mutations as molecular indicators to predict ethambutol susceptibility in mycobacterium tuberculosis. | discordant results in conventional susceptibility testing of ethambutol against mycobacterium tuberculosis may lead to underreporting of drug resistance. | 2012 | 23171739 |
| automatic design of decision-tree induction algorithms tailored to flexible-receptor docking data. | this paper addresses the prediction of the free energy of binding of a drug candidate with enzyme inha associated with mycobacterium tuberculosis. this problem is found within rational drug design, where interactions between drug candidates and target proteins are verified through molecular docking simulations. in this application, it is important not only to correctly predict the free energy of binding, but also to provide a comprehensible model that could be validated by a domain specialist. d ... | 2012 | 23171000 |
| discovery of schaeffer's acid analogues as lead structures of mycobacterium tuberculosis type ii dehydroquinase using a rational drug design approach. | rational ligand design: schaeffer's acid analogues were identified as novel inhibitors of m. tuberculosis type ii dehydroquinase, a key enzyme of the shikimate pathway. their likely binding mode was predicted using a combination of ensemble docking and flexible active site residues. potentially, this scaffold could provide a good starting point for the design of antitubercular agents. | 2013 | 23169689 |
| bioinformatic and empirical analysis of novel hypoxia-inducible targets of the human antituberculosis t cell response. | we analyzed whole genome-based transcriptional profiles of mycobacterium tuberculosis subjected to prolonged hypoxia to guide the discovery of novel potential ags, by a combined bioinformatic and empirical approach. we analyzed the fold induction of the 100 most highly induced genes at 7 d of hypoxia, as well as transcript abundance, peptide-binding prediction (propred) adjusted for population-specific mhc class ii allele frequency, and by literature search. twenty-six candidate genes were selec ... | 2012 | 23169589 |
| extensively drug-resistant tuberculosis in india: a review. | extensively drug resistant tuberculosis (xdr-tb) has become a new threat for the control of tb in many countries including india. its prevalence is not known in india as there is no nation-wide surveillance. however, there have been some reports from various hospitals in the country. | 2012 | 23168700 |
| chemokines shape the immune responses to tuberculosis. | mycobacterium tuberculosis (mtb) is the intracellular pathogen that causes the disease, tuberculosis. chemokines and chemokine receptors are key regulators in immune cell recruitment to sites of infection and inflammation. this review highlights our recent advances in understanding the role of chemokines and chemokine receptors in cellular recruitment of immune cells to the lung, role in granuloma formation and host defense against mtb infection. | 2013 | 23168132 |
| serodiagnosis of tuberculosis in asian elephants (elephas maximus) in southern india: a latent class analysis. | mycobacterium tuberculosis, a causative agent of chronic tuberculosis disease, is widespread among some animal species too. there is paucity of information on the distribution, prevalence and true disease status of tuberculosis in asian elephants (elephas maximus). the aim of this study was to estimate the sensitivity and specificity of serological tests to diagnose m. tuberculosis infection in captive elephants in southern india while simultaneously estimating sero-prevalence. | 2012 | 23166708 |
| clinical utility of two interferon-gamma release assays on pleural fluid for the diagnosis of tuberculous pleurisy. | the release of interferon-gamma (ifn-γ) by t lymphocytes increases after rechallenge with mycobacterium tuberculosis antigen, especially, at a localized site of tuberculosis (tb) infection. we aimed to compare the clincial efficacy of two commercial ifn-γ release assays from pleural fluid for the diagnosis in tuberculous pleurisy. | 2012 | 23166547 |
| role of the mmr efflux pump in drug resistance in mycobacterium tuberculosis. | efflux pumps are membrane proteins capable of actively transporting a broad range of substrates from the cytoplasm to the exterior of the cell. increased efflux activity in response to drug treatment may be the first step in the development of bacterial drug resistance. previous studies showed that the efflux pump mmr was significantly overexpressed in strains exposed to isoniazid. in the work to be described, we constructed mutants lacking or overexpressing mmr in order to clarify the role of t ... | 2013 | 23165464 |
| mechanistic investigation of the oxidation of hydrazides: implications for the activation of the tb drug isoniazid. | aryl hydrazides are oxidised to acyl radicals through a mechanism involving diimide intermediates that are prone to nucleophilic acyl substitution. this oxidation occurs regardless of the oxidant involved, however there is no evidence that the acyl radical formed undergoes further oxidation to the corresponding acylium ion, even in the presence of strong oxidants. this study may provide insight into the mechanism of isoniazid resistance in mycobacterium tuberculosis. | 2013 | 23165368 |
| evaluation of bactec™ mgit™ 960 and the nitrate reductase assay in the national laboratory network of colombia. | the mycobacteria group of the national reference laboratory, national laboratory network, national institute of health and the public health laboratories of antioquia, atlántico and valle del cauca, colombia. | 2013 | 23164219 |
| boron-containing peptidomimetics--a novel class of selective anti-tubercular drugs. | medical treatment for tuberculosis is complicated nowadays by the appearance of new multiresistant strains, and therefore, new antibiotics are in great need. here, we report the synthesis and in vitro testing of a new class of highly selective antimicrobial boron-containing peptidomimetics with compounds exhibiting activity against mycobacterium tuberculosis at ≤5 μg/ml. the new approach developed makes it possible to synthesize variously substituted β-aminoboronic acids and their derivatives wi ... | 2013 | 23164166 |
| immunogenicity and therapeutic effects of ag85a/b chimeric dna vaccine in mice infected with mycobacterium tuberculosis. | the situation of tuberculosis (tb) is very severe in china. new therapeutic agents or regimens to treat tb are urgently needed. in this study, mycobacterium tuberculosis-infected mice were given immunotherapy intramuscularly with ag85a/b chimeric dna or saline, plasmid vector pvax1, or mycobacterium vaccae vaccine. the mice treated with ag85a/b chimeric dna showed significantly higher numbers of t cells secreting interferon-gamma (ifn-γ), more ifn-γ in splenocyte culture supernatant, more th1 an ... | 2012 | 23163873 |
| improved diagnostic power by combined interferon-gamma release assay and nested-pcr in tuberculous pleurisy in high tuberculosis prevalence area. | the conventional acid fast bacilli (afb) smear and mycobacterium tuberculosis (m.tb) culture of pleural effusion and tuberculin skin test (tst) in tuberculous pleurisy are unable to meet clinical needs because of their low sensitivities and specificities. to evaluate the diagnostic accuracies of quantiferon tb gold in-tube test (qft-git) and nested-pcr in tuberculous pleurisy, we conducted a cross-sectional study in regions of china with a high tuberculosis (tb) epidemic. seventy-eight participa ... | 2012 | 23163870 |
| crystal structure of hexanoyl-coa bound to β-ketoacyl reductase fabg4 of mycobacterium tuberculosis. | fabgs, or β-oxoacyl reductases, are involved in fatty acid synthesis. the reaction entails nadph/nadh-mediated conversion of β-oxoacyl-acp (acyl-carrier protein) into β-hydroxyacyl-acp. hmwfabgs (high-molecular-weight fabg) form a phylogenetically separate group of fabg enzymes. fabg4, an hmwfabg from mycobacterium tuberculosis, contains two distinct domains, an n-terminal 'flavodoxintype' domain and a c-terminal oxoreductase domain. the catalytically active c-terminal domain utilizes nadh to re ... | 2013 | 23163771 |
| parotid tuberculosis associated with cutaneous tuberculosis on a medial epicanthus. | an 83-year-old woman presented with a 2-month history of a gradually enlarging, reddish, crusted papule on her left medial epicanthus. her medical history did not reveal any systemic disease. she gave no personal history of tuberculosis or any systemic symptoms, such as night sweat, weight loss, and pulmonary abnormalities. her husband had been treated for pulmonary tuberculosis 30 years ago. a dermatologic examination revealed a 2.5 x 1.2-cm nontender, erythematous plaque with fine, white adher ... | 2012 | 23163079 |
| use of amplified mycobacterium tuberculosis direct test (gen-probe inc., san diego, ca, usa) in the diagnosis of tubercular synovitis and early arthritis of knee joint. | the diagnosis of knee joint tuberculosis, especially in early stages of synovial disease, has more often been based on clinicoradiological suspicion, with no single test claiming to be a dependable rapid diagnostic test with high sensitivity and specificity. nuclear amplification tests in vogue like the polymerase chain reaction have shown variable sensitivity and false positivity rates in various studies. we evaluated the role of amplified mycobacterium tuberculosis direct test (amtdt) or genpr ... | 2012 | 23162145 |
| oxygen migration pathways in no-bound truncated hemoglobin. | atomistic simulations of dioxygen (o(2)) dynamics and migration in nitric oxide-bound truncated hemoglobin n (trhbn) of mycobacterium tuberculosis are reported. from more than 100 ns of simulations the connectivity network involving the metastable states for localization of the o(2) ligand is built and analyzed. it is found that channel i is the primary entrance point for o(2) whereas channel ii is predominantly an exit path although access to the protein active site is also possible. for o(2) a ... | 2012 | 23161831 |
| when inhibitors do not inhibit: critical evaluation of rational drug design targeting chorismate mutase from mycobacterium tuberculosis. | tuberculosis (tb) is a devastating disease that claims millions of lives every year. hindered access or non-compliance to medication, especially in developing countries, led to drug resistance, further aggravating the situation. with current standard therapies in use for over 50 years and only few new candidates in clinical trials, there is an urgent call for new tb drugs. a powerful tool for the development of new medication is structure-guided design, combined with virtual screening or docking ... | 2012 | 23161632 |
| nitric oxide controls the immunopathology of tuberculosis by inhibiting nlrp3 inflammasome-dependent processing of il-1β. | interleukin 1 (il-1) is an important mediator of innate immunity but can also promote inflammatory tissue damage. during chronic infections such as tuberculosis, the beneficial antimicrobial role of il-1 must be balanced with the need to prevent immunopathology. by exogenously controlling the replication of mycobacterium tuberculosis in vivo, we obviated the requirement for antimicrobial immunity and discovered that both il-1 production and infection-induced immunopathology were suppressed by ly ... | 2013 | 23160153 |
| underestimation of the resistance of mycobacterium tuberculosis to second-line drugs by the new genotype mtbdrsl test. | the genotype mtbdrsl is a new-generation pcr-based line-probe assay for the detection of extensively drug-resistant tuberculosis (xdr-tb). this study evaluated the performance of mtbdrsl in detecting genotypic resistance to ethambutol, kanamycin, and ofloxacin in mycobacterium tuberculosis (mtb) strains. the drug resistance of 262 unique clinical mtb isolates from china was analyzed with mtbdrsl, traditional tb drug susceptibility testing (dst), and sequencing. sensitivity of mtbdrsl was 62.4% ( ... | 2013 | 23159109 |
| mycobacterium tuberculosis-driven targeted recalibration of macrophage lipid homeostasis promotes the foamy phenotype. | upon infection, mycobacterium tuberculosis (mtb) metabolically alters the macrophage to create a niche that is ideally suited to its persistent lifestyle. infected macrophages acquire a "foamy" phenotype characterized by the accumulation of lipid bodies (lbs), which serve as both a source of nutrients and a secure niche for the bacterium. while the functional significance of the foamy phenotype is appreciated, the biochemical pathways mediating this process are understudied. we found that mtb in ... | 2012 | 23159056 |
| whole-genome sequencing to delineate mycobacterium tuberculosis outbreaks: a retrospective observational study. | tuberculosis incidence in the uk has risen in the past decade. disease control depends on epidemiological data, which can be difficult to obtain. whole-genome sequencing can detect microevolution within mycobacterium tuberculosis strains. we aimed to estimate the genetic diversity of related m tuberculosis strains in the uk midlands and to investigate how this measurement might be used to investigate community outbreaks. | 2013 | 23158499 |
| [evaluation of tuberculin skin test conversion and interferon-gamma release assay in diagnosis of tuberculosis infection]. | to compare ppd conversion with γ-interferon release assay (igra) in determining the newly mycobacterium tuberculosis (mtb) infection during a tb outbreak. | 2012 | 23158069 |
| [the prevalence of pulmonary tuberculosis in a national survey across china in 2010]. | to investigate the epidemiological trend of pulmonary tuberculosis, and to provide scientific basis for making national tuberculosis control program 2011 - 2015. | 2012 | 23158068 |
| [the study of inter-simple sequences repeat genotyping based on one pentanucleotide repeat sequences in mycobacteria]. | to establish inter-simple sequences repeat (issr) molecular makers based on (cagcg)n repeat sequence in mycobacteria. | 2012 | 23158007 |
| [application and value of fluorescence quantitative pcr in detecting mycobacterium tuberculosis in paraffin embedded specimens]. | | 2012 | 23157752 |
| antigen 85a and mycobacterial dna-binding protein 1 are targets of immunoglobulin g in individuals with past tuberculosis. | development of accurate methods for predicting progression of tuberculosis (tb) from the latent state is recognized as vitally important in controlling tb, because a majority of cases develop from latent infections. past tb that has never been treated has a higher risk of progressing than does latent mycobacterium tuberculosis infection in patients who have previously received treatment. antibody responses against 23 kinds of m. tuberculosis proteins in individuals with past tb who had not been ... | 2013 | 23157580 |
| phyllocladane diterpenes from anisomeles heyneana. | new phyllocladane diterpene, phyllocladan-16α,17-dihydroxy-19-oic acid (1), together with known phyllocladane diterpene, phyllocladan-16α,19-diol (2), cembrane diterpene ovatodiolide (3), sitosteryl-3-o-β-d-glucoside (4), and verbascoside (5), were isolated from aerial parts of anisomeles heyneana. the structure of compound 1 was elucidated by 1d and 2d nmr analyses which included hsqc, hmbc, and nuclear overhauser effect spectroscopy (noesy) experiments as well as x-ray crystallography. this is ... | 2012 | 23157282 |
| [an experimental study of the efficiency of cycloferon in the complex chemotherapy of generalized drug-resistant tuberculosis]. | the results of an experimental study of the efficiency of cycloferon included in a complex chemotherapy of generalized drug-resistant mycobacterium tuberculosis (mbt) are presented. it is established that cycloferon (3.6 mg/kg) produces a significant therapeutic effect, which is manifested by an increase in the lung clearance from mbt, a decrease in the spread of specific inflammation in the lungs, and the disappearance of mbt-induced alterations. in addition, activation of the signs of tension ... | 2012 | 23156086 |
| surviving the macrophage: tools and tricks employed by mycobacterium tuberculosis. | mycobacterium tuberculosis has evolved to withstand one of the most inhospitable cells within the human body, namely the macrophage, a cell that is normally geared toward the destruction of any invading microbe. how m. tuberculosis achieves this is still incompletely understood; however, a number of mechanisms are now known that provide advantages to m. tuberculosis for its survival and proliferation inside the macrophage. while some of these mechanisms are mediated by factors released by m. tub ... | 2013 | 23154833 |
| sudden cardiac arrest caused by tuberculous pericarditis with hemorrhagic pericardial effusion. | as tuberculosis still exists in japan, tuberculous pericarditis is a major health issue. tuberculous pericarditis is difficult to diagnose and leads to poor outcomes when left untreated. we herein report the case of a patient who was admitted to the hospital after undergoing resuscitation for cardiopulmonary arrest. mycobacterium tuberculosis was detected in his hemorrhagic pericardial fluid and tuberculous pericarditis was diagnosed. the administration of antituberculous medication resulted in ... | 2012 | 23154733 |
| the role of dendritic cells in mycobacterium tuberculosis infection. | the immune response against mycobacterium tuberculosis is multifactorial, involving a network of innate and adaptive immune responses. characterization of the immune response, a clear understanding of the dynamics and interplay of different arms of the immune response are critical to allow the development of better tools for combating tuberculosis. dendritic cells (dcs) are one of the key cells in bridging innate and adaptive immune response through their significant role in capturing, processin ... | 2012 | 23154283 |
| diagnostic accuracy of the genotype mtbdrsl assay for rapid diagnosis of extensively drug-resistant tuberculosis in hiv-coinfected patients. | the russian federation is a high-tuberculosis (tb)-burden country with high rates of mycobacterium tuberculosis multidrug resistance (mdr) and extensive drug resistance (xdr), especially in hiv-coinfected patients. rapid and reliable diagnosis for detection of resistance to second-line drugs is vital for adequate patient management. we evaluated the performance of the genotype mtbdrsl (hain lifescience gmbh, nehren, germany) assay on smear-positive sputum specimens obtained from 90 hiv-infected ... | 2013 | 23152552 |
| crowding, molecular volume and plasticity: an assessment involving crystallography, nmr and simulations. | the discrepancy between the x-ray and nmr structures of mycobacterium tuberculosis peptidyl-trna hydrolase in relation to the functionally important plasticity of the molecule led to molecular dynamics simulations. the x-ray and the nmr studies along with the simulations indicated an inverse correlation between crowding and molecular volume. a detailed comparison of proteins for which x-ray and the nmr structures appears to confirm this correlation. in consonance with the reported results of the ... | 2012 | 23151785 |
| x-ray study of the conformational changes in the molecule of phosphopantetheine adenylyltransferase from mycobacterium tuberculosis during the catalyzed reaction. | structures of recombinant phosphopantetheine adenylyltransferase (ppat) from mycobacterium tuberculosis (ppatmt) in the apo form and in complex with the substrate atp were determined at 1.62 and 1.70 å resolution, respectively, using crystals grown in microgravity by the counter-diffusion method. the atp molecule of the ppatmt-atp complex was located with full occupancy in the active-site cavity. comparison of the solved structures with previously determined structures of ppatmt complexed with t ... | 2012 | 23151631 |
| molecular hybridization of bioactives: synthesis and antitubercular evaluation of novel dibenzofuran embodied homoisoflavonoids via baylis-hillman reaction. | a novel series of natural product like dibenzofuran embodied homoisoflavonoids [(e)-3-(dibenzo[b,d]furan-2-ylmethylene)chroman-4-ones] designed by molecular hybridization were synthesized in very good yields via a sequence of reactions involving base catalyzed baylis-hillmann (bh) reaction of 2-dibenzofuran carboxaldehyde and methyl acrylate; bromination of bh adduct; condensation of resulted allylic bromide with substituted phenols or 2-dibenzofuranol followed by cyclization. among the all 11 n ... | 2012 | 23151429 |
| rapid and accurate detection of rmp- and inh- resistant mycobacterium tuberculosis in spinal tuberculosis specimens by capitalbio™ dna microarray: a prospective validation study. | dna microarrays can detect tuberculosis and its multi-drug resistant form in m. tuberculosis isolates and sputum specimens with high sensitivity and specificity. however, no performance data currently exists for its use in spinal tuberculosis specimens. this study was aimed to assess the performance of the capitalbio™ dna microarray in the detection of isoniazid (inh) and rifampicin (rmp) resistance in spinal tuberculosis compared with the bact/mgit 960 system. | 2012 | 23151186 |
| synthesis and antitubercular activity of 2-(substituted phenyl/benzyl-amino)-6-(4-chlorophenyl)-5-(methoxycarbonyl)-4-methyl-3,6-dihydropyrimidin-1-ium chlorides. | a series of 2-(substituted phenyl/benzyl-amino)-6-(4-chlorophenyl)-5-(methoxycarbonyl)-4-methyl-3,6-dihydropyrimidin-1-ium chlorides 7-13 and 15 was synthesized in their hydrochloride salt form. the title compounds were characterized by ft-ir, nmr ((1)h and (13)c) and elemental analysis. they were evaluated for their in vitro antitubercular activity against mycobacterium tuberculosis h37rv, multidrug resistance tuberculosis and extensively drug resistance tuberculosis by agar diffusion method an ... | 2013 | 23150983 |
| potent activity against multidrug-resistant mycobacterium tuberculosis of α-mangostin analogs. | a new series of mangostin analogs of natural α-mangostin from mangosteen was prepared and their antimycobacterial activity was evaluated in vitro against mycobacterium tuberculosis h(37)ra. the results showed that the monoalkyl tetrahydro α-mangostin analogs displayed increased antimycobacterial activity as compared with the lead natural xanthone, α-mangostin. among the tested compounds, 6-methoxytetrahydro α-mangostin (16) exhibited the most potent antimycobacterial activity with minimum inhibi ... | 2013 | 23150066 |
| bis-imidazolinylindoles are active against methicillin-resistant staphylococcus aureus and multidrug-resistant mycobacterium tuberculosis. | | 2013 | 23149517 |
| characterization of rifampin-resistant isolates of mycobacterium tuberculosis from sichuan in china. | rifampin is a key component of standard short-course first-line therapy against mycobacterium tuberculosis, and rifampin resistance of this pathogen has been reported to be related to rpob gene mutations. the objective of this study was to characterize the rpob gene mutations in rifampin-resistant m. tuberculosis isolates circulated in sichuan. sequencing of rpob gene and spoligotyping were performed on 268 randomly selected isolates from january 2008 to may 2010. the results indicated that 207 ... | 2013 | 23149305 |
| structural basis of the binding of fatty acids to peptidoglycan recognition protein, pgrp-s through second binding site. | short peptidoglycan recognition protein (pgrp-s) is a member of the mammalian innate immune system. pgrp-s from camelus dromedarius (cpgrp-s) has been shown to bind to lipopolysaccharide (lps), lipoteichoic acid (lta) and peptidoglycan (pgn). its structure consists of four molecules a, b, c and d with ligand binding clefts situated at a-b and c-d contacts. it has been shown that lps, lta and pgn bind to cpgrp-s at c-d contact. the cleft at the a-b contact indicated features that suggested a poss ... | 2013 | 23149273 |
| multifunctional immune responses of hmbpp-specific vγ2vδ2 t cells in m. tuberculosis and other infections. | vγ2vδ2 t (also known as vγ9vδ2 t) cells exist only in primates, and in humans represent a major γδ t-cell sub-population in the total population of circulating γδ t cells. results from recent studies suggest that while (e)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (hmbpp) phosphoantigen from mycobacterium tuberculosis (mtb) and other microbes activates and expands primate vγ2vδ2 t cells, the vγ2vδ2 t-cell receptor (tcr) recognizes and binds to hmbpp on antigen-presenting cells (apc). in respon ... | 2013 | 23147720 |
| relationship between chemokine receptor expression, chemokine levels and hiv-1 replication in the lungs of persons exposed to mycobacterium tuberculosis. | increased susceptibility to tuberculosis following hiv-1 seroconversion contributes significantly to the tuberculosis epidemic in sub-saharan africa. lung-specific mechanisms underlying the interaction between hiv-1 and mycobacterium tuberculosis infection are incompletely understood. here we address these questions by examining the effect of hiv-1 and latent m. tuberculosis co-infection on the expression of viral-entry receptors and ligands in bronchoalveolar lavage (bal) of hiv-1-infected and ... | 2013 | 23147374 |
| tuberculosis in a southern brazilian prison. | the occurrence of tuberculosis (tb) in prisons has been described as an alarming public health problem in many countries, especially in developing nations. the objective of this study was to conduct a survey among prisoners with tb respiratory symptoms in order to estimate the incidence of the disease, to analyze the drug susceptibility profile and genotype the isolates of mycobacterium tuberculosis in the city of charqueadas, southern of brazil. the tb incidence was 55/1,900 inhabitants in the ... | 2012 | 23147148 |
| fast test for assessing the susceptibility of mycobacterium tuberculosis to isoniazid and rifampin by real-time pcr. | mycobacterium tuberculosis is the bacterium that causes tuberculosis (tb), a leading cause of death from infectious disease worldwide. rapid diagnosis of resistant strains is important for the control of tb. real-time polymerase chain reaction (rt-pcr) assays may detect all of the mutations that occur in the m. tuberculosis 81-bp core region of the rpob gene, which is responsible for resistance to rifampin (rif) and codon 315 of the katg gene and the inha ribosomal binding site, which are respon ... | 2012 | 23147147 |
| adverse neuro-immune-endocrine interactions in patients with active tuberculosis. | the nervous, endocrine and immune systems play a crucial role in maintaining homeostasis and interact with each other for a successful defensive strategy against injurious agents. however, the situation is different in long-term diseases with marked inflammation, in which defensive mechanisms become altered. in the case of tuberculosis (tb), this is highlighted by several facts: an imbalance of plasma immune and endocrine mediators, that results in an adverse environment for mounting an adequate ... | 2013 | 23147110 |
| correlation between resistance to pyrazinamide and resistance to other antituberculosis drugs in mycobacterium tuberculosis strains isolated at a referral hospital. | the correlation between resistance to pyrazinamide (pza) and resistance to other first-line antituberculosis drugs was investigated in 395 mycobacterium tuberculosis strains isolated from clinical specimens, representing 14% of the overall number of m. tuberculosis isolates obtained between 2003 and 2008 at the laboratory of a referral university hospital for tuberculosis. a high correlation was found between resistance to pza and multidrug resistance, as well as between pza resistance and resis ... | 2012 | 23147056 |
| understanding tb latency using computational and dynamic modelling procedures. | the mycobacterium tuberculosis bacilli's potency to cause persistent latent infection that is unresponsive to the current cocktail of tb drugs is strongly associated with its ability to adapt to changing intracellular environments, and tolerating, evading and subverting host defence mechanisms. we applied a combination of bioinformatics and mathematical modelling methods to enhance the understanding of tb latency dynamics. analysis of time course microarray gene expression data was carried out a ... | 2013 | 23146828 |
| clinical features and outcomes of tuberculosis in solid organ transplant recipients. | tuberculosis (tb) remains a significant opportunistic infection in solid organ transplant (sot) recipients. moreover, its optimal treatment in sot recipients is challenging due to the toxicity and potential drug-drug interactions of antituberculus drugs. we sought to assess the frequency, clinical characteristics, treatments, and outcomes of tb among sot recipients. | 2012 | 23146494 |
| characterization of extensively drug-resistant mycobacterium tuberculosis in nepal. | the emergence of extensively drug-resistant tuberculosis (xdr-tb) has raised public health concern for global control of tb. although molecular characterization of drug resistance-associated mutations in multidrug-resistant isolates in nepal has been made, mutations in xdr isolates and their genotypes have not been reported previously. in this study, we identified and characterized 13 xdr mycobacterium tuberculosis isolates from clinical isolates in nepal. the most prevalent mutations involved i ... | 2013 | 23146281 |
| fadd3 is an acyl-coa synthetase that initiates catabolism of cholesterol rings c and d in actinobacteria. | the cholesterol catabolic pathway occurs in most mycolic acid-containing actinobacteria, such as rhodococcus jostii rha1, and is critical for mycobacterium tuberculosis (mtb) during infection. fadd3 is one of four predicted acyl-coa synthetases potentially involved in cholesterol catabolism. a δfadd3 mutant of rha1 grew on cholesterol to half the yield of wild-type and accumulated 3aα-h-4α(3'-propanoate)-7aβ-methylhexahydro-1,5-indanedione (hip), consistent with the catabolism of half the steroi ... | 2013 | 23146019 |
| role of phee15 gate in ligand entry and nitric oxide detoxification function of mycobacterium tuberculosis truncated hemoglobin n. | the truncated hemoglobin n, hbn, of mycobacterium tuberculosis is endowed with a potent nitric oxide dioxygenase (nod) activity that allows it to relieve nitrosative stress and enhance in vivo survival of its host. despite its small size, the protein matrix of hbn hosts a two-branched tunnel, consisting of orthogonal short and long channels, that connects the heme active site to the protein surface. a novel dual-path mechanism has been suggested to drive migration of o(2) and no to the distal he ... | 2012 | 23145144 |
| immune responses to esat-6 and cfp-10 by fascia and multiplex technology for diagnosis of m. tuberculosis infection; ip-10 is a promising marker. | there is a need for reliable markers to diagnose active and latent tuberculosis (tb). the interferon gamma release assays (igras) are compared to the tuberculin skin test (tst) more specific, but cannot discriminate between recent or remote tb infection. here the flow-cytometric assay for specific cell-mediated immune-response in activated whole blood (fascia), which quantifies expanded t-lymphoblasts by flow-cytometric analysis after long-term antigen stimulation of whole blood, is combined wit ... | 2012 | 23144772 |
| tim-3-expressing cd4+ and cd8+ t cells in human tuberculosis (tb) exhibit polarized effector memory phenotypes and stronger anti-tb effector functions. | t-cell immune responses modulated by t-cell immunoglobulin and mucin domain-containing molecule 3 (tim-3) during mycobacterium tuberculosis (mtb) infection in humans remain poorly understood. here, we found that active tb patients exhibited increases in numbers of tim-3-expressing cd4(+) and cd8(+) t cells, which preferentially displayed polarized effector memory phenotypes. consistent with effector phenotypes, tim-3(+)cd4(+) and tim-3(+)cd8(+) t-cell subsets showed greater effector functions fo ... | 2012 | 23144609 |
| computational finding of potential inhibitor for cytochrome p450 mono-oxygenases enzyme of mycobacterium tuberculosis. | cytochrome p450 mono-oxygenases (2uuq) enzyme from mycobacterium tuberculosis catalyzes oxidation of organic compounds such as lipids and steroidal hormones therefore remain as potential drug target. currently available first line anti-tuberculosis drugs have been caused several side effects in the body as well as resistance development by mycobacterium against these drugs, necessitates the considerable need for finding new drugs. therefore, we propose a structure based computational method to f ... | 2012 | 23144553 |
| computational validation of 3-ammonio-3-(4-oxido- 1h-imidazol-1-ium-5-yl) propane-1, 1-bis (olate) as a potent anti-tubercular drug against mt-metap. | the advent of multi drug resistant (mdr) strain of mycobacterium tuberculosis (tb) necessitated search for new drug targets for the bacterium. it is reported that 3.3% of all new tuberculosis cases had multidrug resistance (mdr-tb) in 2009 and each year, about 0.44 million mdr-tb cases are estimated to emerge and 0.15 million people with mdr-tb die. keeping such an alarming situation under consideration we wanted to design suitable anti tubercular molecules for new target using computational too ... | 2012 | 23144543 |
| differential arabinan capping of lipoarabinomannan modulates innate immune responses and impacts t helper cell differentiation. | toll-like receptors (tlrs) recognize pathogens by interacting with pathogen-associated molecular patterns, such as the phosphatidylinositol-based lipoglycans, lipomannan (lm) and lipoarabinomannan (lam). such structures are present in several pathogens, including mycobacterium tuberculosis, being important for the initiation of immune responses. it is well established that the interaction of lm and lam with tlr2 is a process dependent on the structure of the ligands. however, the implications of ... | 2012 | 23144457 |
| mycobacterium tuberculous peritonitis in capd patients: a report of 11 patients and review of literature. | the aims of the present report were to document our experience of the prevalence of tuberculous peritonitis in continuous ambulatory peritoneal dialysis (capd) patients, mode of presentation, diagnosis and outcome and to discuss the current published data about catheter removal. | 2013 | 23143752 |
| targeting multidrug-resistant tuberculosis (mdr-tb) by therapeutic vaccines. | tuberculosis (tb) has scourged humankind for millennia, and latent infection affects nearly one-third of today's world population. the emergence of multidrug-resistant (mdr)-tb is a major global threat and reflects treatment failure of drug-sensitive disease. mdr-tb management is a burden for patients and society; success rates are unacceptably low with prolonged treatment duration. mycobacterium tuberculosis (mtb) possesses the ability to transform into a dormant state in which it can persist i ... | 2013 | 23143437 |
| the expression of abc efflux pump, rv1217c-rv1218c, and its association with multidrug resistance of mycobacterium tuberculosis in china. | currently the treatment of mycobacterium tuberculosis (tb) infection is largely limited due to the prevalence of multidrug resistance strains. over-expressing the efflux pumps such as the atp-binding cassette (abc) transporter has been reported to significantly contribute to its resistance to several antibiotics. this study investigated the expression profile of one important abc efflux pump, rv1217c-rv1218c, by quantitative real-time pcr (rt-qpcr) in clinical isolates from china, which also rev ... | 2013 | 23143285 |
| gastric aspiration is not necessary for the diagnosis of pulmonary tuberculosis. | despite recommendations, gastric aspirate collected by invasive nasogastric aspiration is still routinely used for the direct detection of mycobacterium tuberculosis in our institution. reviewing 82 patients with culture-proven respiratory tuberculosis over a 28-month period, we observed no patient diagnosed solely by gastric aspirate analysis. moreover, the diagnosis yield of gastric aspirate (60 %) did not significantly differ from that of stool specimen (64 %). these data confirm that gastric ... | 2013 | 23143083 |
| chitosan microspheres as an alveolar macrophage delivery system of ofloxacin via pulmonary inhalation. | because mycobacterium tuberculosis, which causes tuberculosis, survives mainly in the alveolar macrophages, the remedial efficiency of anti-tuberculosis drugs such as ofloxacin may be improved by their direct delivery to the lungs via pulmonary inhalation. for this purpose, ofloxacin-loaded, glutaraldehyde-crosslinked chitosan microspheres (ocms) were prepared using a water-in-oil emulsification method. the particle size of the ocms was around 1-6 μm, and the content of ofloxacin was 27% (w/w). ... | 2013 | 23142421 |
| activity of ll-37, cramp and antimicrobial peptide-derived compounds e2, e6 and cp26 against mycobacterium tuberculosis. | tuberculosis (tb) is a major worldwide health problem in part due to the lack of development of new treatments and the emergence of new strains such as multidrug-resistant (mdr) and extensively drug-resistant strains that are threatening and impairing the control of this disease. in this study, the efficacy of natural and synthetic cationic antimicrobial (host defence) peptides that have been shown often to possess broad-spectrum antimicrobial activity was tested. the natural antimicrobial pepti ... | 2013 | 23141114 |
| antibody response to mycobacterium tuberculosis p27-ppe36 antigen in sera of pulmonary tuberculosis patients. | we have expressed, characterized and studied the immune response of mice against the mycobacterium tuberculosis ppe36 protein. this protein encoded by the gene rv2108 is a 27-kda cell-wall associated protein. here, we compare the antibody isotypes distribution of p27-ppe36 in sera of pulmonary tuberculosis patients and in sera from healthy control subjects by enzyme-linked immunosorbent assay (elisa). we detected in the sera of affected individuals a significant increase of iga antibody response ... | 2013 | 23140854 |
| molecular characterization of mycobacterium tuberculosis isolates from north indian patients with extrapulmonary tuberculosis. | genotypic studies are important to understand the molecular epidemiology and transmission routes of mycobacterium tuberculosis. in the first and largest study from india, spoligotyping and 24 loci mycobacterial interspersed repetitive units (miru) were performed to find genetic profiles of 125 m. tuberculosis strains isolated from patients with extrapulmonary tuberculosis (eptb) and their drug susceptibility test was performed using bactec-mgit 960. spoligotyping results were compared with the w ... | 2013 | 23140853 |
| novel insights into mycobacterium antigen ag85 biology and implications in countermeasures for m. tuberculosis. | tuberculosis remains one of the most prevalent and deadly infectious diseases, largely due to the emergence of multidrug-resistant and extensive drug-resistant mycobacterium tuberculosis, especially the coinfection with hiv. mycobacterium ag85 complex (ag85a, b, and c), with a carboxylesterase consensus sequence and conserved surface catalysis residues, involves in cell wall biosynthesis and the trigger of the host immune response. the physiological function, structures, distributions, and molec ... | 2012 | 23140159 |
| allosteric competitive inhibitors of the glucose-1-phosphate thymidylyltransferase (rmla) from pseudomonas aeruginosa. | glucose-1-phosphate thymidylyltransferase (rmla) catalyzes the condensation of glucose-1-phosphate (g1p) with deoxy-thymidine triphosphate (dttp) to yield dtdp-d-glucose and pyrophosphate. this is the first step in the l-rhamnose biosynthetic pathway. l-rhamnose is an important component of the cell wall of many microorganisms, including mycobacterium tuberculosis and pseudomonas aeruginosa. here we describe the first nanomolar inhibitors of p. aeruginosa rmla. these thymine analogues were ident ... | 2013 | 23138692 |
| development of 5-nitrothiazole derivatives: identification of leads against both replicative and latent mycobacterium tuberculosis. | twenty eight 5-nitrothiazole derivatives were synthesized and evaluated for in vitro activities against mycobacterium tuberculosis (mtb), cytotoxicity against hek 293t. among the compounds, 5-nitro-n-(5-nitrothiazol-2-yl)furan-2-carboxamide (20) was found to be the most active compound in vitro with mics of 5.48 μm against log-phase culture of mtb and also non-toxic up to 100 μm. | 2012 | 23137434 |
| mycobacterium tuberculosis region of difference (rd) 2 antigen rv1985c and rd11 antigen rv3425 have the promising potential to distinguish patients with active tuberculosis from m. bovis bcg-vaccinated individuals. | antigens encoded in the region of difference (rd) of mycobacterium tuberculosis constitute a potential source of specific immunodiagnostic antigens for distinguishing tuberculosis (tb) infection from bcg vaccination. we evaluated the diagnostic potential of specific t-cell epitopes selected from two immunodominant antigens, rv1985c and rv3425, from rd2 and rd11, respectively, on the basis of epitope mapping, in tb patients and bcg-vaccinated healthy individuals. using a whole-blood gamma interfe ... | 2013 | 23136116 |
| mycobacterium tuberculosis requires phosphate-responsive gene regulation to resist host immunity. | mycobacterium tuberculosis persists in the tissues of mammalian hosts despite inducing a robust immune response dominated by the macrophage-activating cytokine gamma interferon (ifn-γ). we identified the m. tuberculosis phosphate-specific transport (pst) system component psta1 as a factor required to resist ifn-γ-dependent immunity. a δpsta1 mutant was fully virulent in ifn-γ(-/-) mice but attenuated in wild-type (wt) mice and mice lacking specific ifn-γ-inducible immune mechanisms: nitric oxide ... | 2013 | 23132496 |
| substituted n-benzylpyrazine-2-carboxamides: synthesis and biological evaluation. | a series of twelve amides was synthesized via aminolysis of substituted pyrazinecarboxylic acid chlorides with substituted benzylamines. compounds were characterized with analytical data and assayed in vitro for their antimycobacterial, antifungal, antibacterial and photosynthesis-inhibiting activity. 5-tert-butyl-6-chloro-n-(4-methoxybenzyl)pyrazine-2-carboxamide (12) has shown the highest antimycobacterial activity against mycobacterium tuberculosis (mic = 6.25 µg/ml), as well as against ... | 2012 | 23132136 |
| reduced mycobacterium tuberculosis association with monocytes from diabetes patients that have poor glucose control. | the re-emerging importance of type 2 diabetes mellitus (dm) to tuberculosis (tb) control is of growing concern, but the basis for this relationship is poorly understood. given the importance of mononuclear phagocytes for tb control and the reported alterations in monocytes of dm patients, we evaluated whether the initial interaction between both was affected in diabetics. mycobacterium tuberculosis-naïve individuals with and without dm were group matched by age and gender and the efficiency of m ... | 2013 | 23131496 |
| anti-tuberculosis activity of indian grass khus (vetiveria zizanioides l. nash). | vetiveria zizanioides l. nash (family: poaceae) root (intact and spent) extracts and fractions were evaluated for antimycobacterial activity against mycobacterium tuberculosis h(37)rv and h(37)ra strains using radiometric bactec 460 tb system. | 2012 | 23131375 |
| nucleic acid amplification test and bronchoscopy improve the diagnostic accuracy of smear-negative tuberculosis. | to determine whether the nucleic acid amplification (naa) test on specimens collected by bronchoscopy improves the diagnostic accuracy of pulmonary tuberculosis (ptb) in sputum-negative patients. | 2012 | 23131268 |
| prolonged positivity of sputum smears with negative cultures during treatment for pulmonary tuberculosis. | acid-fast bacilli (afb) microscopy of sputum smears is the most widely used tool for both diagnosing pulmonary tuberculosis (ptb) and monitoring treatment response. it is not uncommon for patients who show clinical improvement to have prolonged positivity of sputum smears (i.e., ≥60 days after initiation of treatment) with corresponding negative cultures. | 2012 | 23131266 |
| risk factors for mycobacterium tuberculosis infection in indonesian children living with a sputum smear-positive case. | young children living with infectious tuberculosis (tb) cases are at high risk of infection and disease, and screening is recommended. this is rarely conducted in resource-limited settings. identifying children most at risk of infection may be useful for setting practical screening policies. | 2012 | 23131256 |
| a first insight into the genotypic diversity of mycobacterium tuberculosis from rwanda. | | 2012 | 23131092 |
| mannose-capped lipoarabinomannan from mycobacterium tuberculosis preferentially inhibits sphingosine-1-phosphate-induced migration of th1 cells. | chemokine receptor cross-desensitization provides an important mechanism to regulate immune cell recruitment at sites of inflammation. we previously reported that the mycobacterial cell wall glycophospholipid mannose-capped lipoarabinomannan (manlam) could induce human peripheral blood t cell chemotaxis. therefore, we examined the ability of manlam to desensitize t cells to other chemoattractants as a potential mechanism for impaired t cell homing and delayed lung recruitment during mycobacteria ... | 2012 | 23129756 |
| when is an outbreak not an outbreak? fit, divergent strains of mycobacterium tuberculosis display independent evolution of drug resistance in a large london outbreak. | to study the evolutionary relationship of mycobacterium tuberculosis isolates from 13 patients in a large outbreak of isoniazid-resistant tuberculosis in london. | 2013 | 23129727 |
| diverse antigen presentation by the group 1 cd1 molecule, cd1c. | cd1 molecules are major histocompatibility complex (mhc) class i-like proteins that present diverse lipid antigens to t cells. most of our understanding of cd1 lipid presentation and t cell recognition has come from study of the invariant natural killer t cell recognition of cd1d. however, in addition to cd1d, humans possess three additional cd1 molecules: cd1a, cd1b and cd1c, referred to as the group 1 cd1s. the lack of an appropriate murine molecule to probe the function and disease relevance ... | 2013 | 23127489 |
| enhanced serodiagnostic utility of novel mycobacterium tuberculosis polyproteins. | the detection of mycobacterium tuberculosis specific antibodies in human sera has been a rapid and important diagnostic aid for tuberculosis (tb) control and prevention. however, any single antigen is not enough to be used to cover the antibody profiles of all tb patients. | 2013 | 23127349 |
| phosphorylation of mitogen-activated protein kinases contributes to interferon γ production in response to mycobacterium tuberculosis. | immune control of mycobacterium tuberculosis depends on interferon γ (ifn-γ)-producing cd4(+) lymphocytes. previous studies have shown that t cells from patients with tuberculosis produce less ifn-γ, compared with healthy donors, in response to mycobacterial antigens, although ifn-γ responses to mitogens are preserved. in this work, we found that m. tuberculosis-induced ifn-γ production by human t cells correlated with phosphorylation of the mitogen-activated protein kinases (mapks), extracellul ... | 2013 | 23125442 |
| global analysis of mrna stability in mycobacterium tuberculosis. | mycobacterium tuberculosis (mtb) is a highly successful pathogen that infects over a billion people. as with most organisms, mtb adapts to stress by modifying its transcriptional profile. remodeling of the transcriptome requires both altering the transcription rate and clearing away the existing mrna through degradation, a process that can be directly regulated in response to stress. to understand better how mtb adapts to the harsh environs of the human host, we performed a global survey of the ... | 2013 | 23125364 |
| a regio- and stereoselective 1,3-dipolar cycloaddition for the synthesis of new-fangled dispiropyrrolothiazoles as antimycobacterial agents. | a series of dispiropyrrolothiazoles compounds were synthesized using 1,3-dipolar cycloaddition and were screened for antimycobacterial activity against mycobacterium tuberculosis h(37)rv and inh resistant m. tuberculosis strains. two of them were showing good activity with mic of less than 1 μm. compound (5f) was found to be the most active with mic of 0.210 and 8.312 μm respectively. | 2012 | 23122863 |
| microwave-assisted synthesis, molecular docking and antitubercular activity of 1,2,3,4-tetrahydropyrimidine-5-carbonitrile derivatives. | based on bioisosteric similarities with isoniazid, a series of 1,2,3,4-tetrahydropyrimidine-5-carbonitrile derivatives has been designed. the target compounds have been synthesized by multicomponent reaction which involves one-pot organic reactions using ethylcyanoacetate, urea/thiourea and arylaldehydes in presence of ethanolic k(2)co(3). two methodologies, conventional and microwave-assisted, have been adopted for the synthesis. the later strategy gave high yields in less than 10 min as compar ... | 2012 | 23122523 |
| [glandular tuberculosis as the cause of dysphagia]. | tuberculosis without pulmonal manifestation is uncommon, but must still be considered as differential diagnosis of lymphadenopathy. we present a case where the main symptom was dysphagia caused by a traction diverticulum due to glandular tuberculosis, which involved the lymph nodes in the neck. based on the results of a magnetic resonance scanning and a computed tomography the primary diagnosis was a malignant oesophageal tumour, but a polymerase chain reaction on needle-aspirated pus from a lym ... | 2012 | 23121911 |
| [continued problems with tuberculosis among danes and greenlanders in denmark and the need for reinforced control--a systematic review]. | active transmission of mycobacterium tuberculosis (mt) continues at surprisingly high rates in denmark. the transmission is particularly observed in specific high risk segments of the population with social problems such as homelessness, alcohol, and/or drug abuse. the patients are infected with the "danish cluster 2" mt outbreak strain, and the transmission is attributed to delayed diagnosis. this situation demands increased focus on early tuberculosis diagnosis, control of transmission, and im ... | 2012 | 23121905 |
| the balance of apoptotic and necrotic cell death in mycobacterium tuberculosis infected macrophages is not dependent on bacterial virulence. | an important mechanism of mycobacterium tuberculosis pathogenesis is the ability to control cell death pathways in infected macrophages: apoptotic cell death is bactericidal, whereas necrotic cell death may facilitate bacterial dissemination and transmission. | 2012 | 23118880 |
| para-aminosalicylic acid acts as an alternative substrate of folate metabolism in mycobacterium tuberculosis. | folate biosynthesis is an established anti-infective target, and the antifolate para-aminosalicylic acid (pas) was one of the first anti-infectives introduced into clinical practice on the basis of target-based drug discovery. fifty years later, pas continues to be used to treat tuberculosis. pas is assumed to inhibit dihydropteroate synthase (dhps) in mycobacterium tuberculosis by mimicking the substrate p-aminobenzoate (paba). however, we found that sulfonamide inhibitors of dhps inhibited gro ... | 2013 | 23118010 |
| point mutations within the fatty acid synthase type ii dehydratase components hada or hadc contribute to isoxyl resistance in mycobacterium tuberculosis. | the mechanism by which the antitubercular drug isoxyl (iso) inhibits mycolic acid biosynthesis has not yet been reported. we found that point mutations in either the hada or hadc component of the type ii fatty acid synthase (fas-ii) are associated with increased levels of resistance to iso in mycobacterium tuberculosis. overexpression of the hadab, hadbc, or hadabc heterocomplex also produced high-level resistance. these results show that the fas-ii dehydratases are involved in iso resistance. | 2013 | 23114755 |
| comparison of smear microscopy, culture, and real-time pcr for quantitative detection of mycobacterium tuberculosis in clinical respiratory specimens. | as a rapid diagnostic technique, the real-time polymerase chain reaction (pcr) can detect mycobacterium tuberculosis with a high sensitivity and specificity. however, further studies are needed to confirm it as a standard method. in this study, we evaluated the cyp141 gene for the detection and quantification of m. tuberculosis in respiratory specimens and compared the results with direct microscopy and culture. | 2013 | 23113553 |
| immunocytochemistry versus nucleic acid amplification in fine needle aspirates and tissues of extrapulmonary tuberculosis. | immunocytochemistry (icc) is an established routine diagnostic adjunct to cytology and histology for tumor diagnosis but has received little attention for diagnosis of tuberculosis. | 2012 | 23112454 |
| testicular tuberculosis in multidrug-resistant pulmonary tuberculosis. | we report a rare case of multidrug-resistant pulmonary and testicular tuberculosis (tb) in a 25-year-old immunocompetent patient. the patient was suspected to have a testicular cancer. he underwent radical orchiectomy, and surgical pathology revealed a granuloma containing acid-fast bacilli in the testis. bronchial washing fluid culture grew mycobacterium tuberculosis resistant to isoniazid, rifampin, and ethambutol. to our knowledge, this patient represents the first case of testicular tb in mu ... | 2013 | 23111433 |
| tat-dependent translocation of an f420-binding protein of mycobacterium tuberculosis. | f(420) is a unique cofactor present in a restricted range of microorganisms, including mycobacteria. it has been proposed that f(420) has an important role in the oxidoreductive reactions of mycobacterium tuberculosis, possibly associated with anaerobic survival and persistence. the protein encoded by rv0132c has a predicted n-terminal signal sequence and is annotated as an f(420)-dependent glucose-6-phosphate dehydrogenase. here we show that rv0132c protein does not have the annotated activity. ... | 2012 | 23110042 |
| pkne, a serine/threonine protein kinase from mycobacterium tuberculosis has a role in adaptive responses. | serine/threonine protein kinases (stpk) play a major role in the physiology and pathogenesis of mycobacterium tuberculosis. here, we have examined the role of pkne, a stpk in the adaptive responses of m. tuberculosis using a deletion mutant δpkne. the survival of δpkne was assessed in the presence of stress (ph, surfactant and cell wall-damaging agents) and anti-tuberculosis drugs. δpkne had a defective growth in ph 7.0 and lysozyme (a cell wall-damaging agent) with better survival in ph 5.5, sd ... | 2013 | 23108860 |