| cr(iii), fe(iii) and co(iii) complexes of tetradentate (onno) schiff base ligands: synthesis, characterization, properties and biological activity. | a series of metal complexes were synthesized from equimolar amounts of schiff bases: 1,4-bis[3-(2-hydroxy-1-naphthaldimine)propyl]piperazine (bappnaf) and 1,8-bis[3-(2-hydroxy-1-naphthaldimine)-p-menthane (damnaf) with metal chlorides. all of synthesized compounds were characterized by elemental analyses, spectral (uv-vis, ir, (1)h-(13)c nmr, lc-ms) and thermal (tga-dta) methods, magnetic and conductance measurements. schiff base complexes supposed in tetragonal geometry have the general formula ... | 2008 | 17904895 |
| antibacterial activity of a bacteriocin-like substance produced by bacillus sp. p34 that targets the bacterial cell envelope. | the objective of this study was to investigate the mode of action of bls p34, a bacteriocin-like substance (bls) produced by a novel bacillus sp. strain p34 isolated from the amazon basin. the effect of the bls was tested against listeria monocytogenes, showing a bactericidal effect at 200 au (activity units) ml(-1), while no inhibition of spore outgrowth of bacillus cereus was observed with a dose of 1,600 au ml(-1). growth of escherichia coli and salmonella enteritidis was inhibited, but only ... | 2008 | 17906937 |
| effect of heat-generated product from uronic acids on the physiological activities of microbial cells and its application. | filtered samples of monogalacturonic (ga) and monoglucuronic acids (gl) that were prepared using millipore filter (pore size=0.2 microm) slightly inhibited the growth of escherichia coli while the autoclaved (at 121 degrees c for 20 min) samples of ga and gl completely inhibited the growth of e. coli. the most effective substance generated upon autoclave treatment was isolated and characterized as trans-4,5-dihydroxy-2-cyclopenten-1-one (dhcp). the optimal conditions for dhcp generation were als ... | 2008 | 17911011 |
| carbohydrazones of substituted salicylaldehydes as potential lead compounds for the development of narrow-spectrum antimicrobials. | certain substituted salicylaldehydes are known to have highly potent antimicrobial activity against bacteria and fungi, but the mechanism underlying this remarkable activity is not known, and almost nothing has been reported on the effects of further modification of the structures, such as the formation of hydrazone-type derivatives. we report now a study on the antimicrobial properties of the carbohydrazone derivatives of several substituted salicylaldehydes. the compounds studied were synthesi ... | 2007 | 17913061 |
| antimicrobial properties of substituted salicylaldehydes and related compounds. | a systematic survey of the antimicrobial properties of substituted salicylaldehydes and some related aromatic aldehydes is reported. a total of 23 different compounds, each at four different concentrations, were studied using a panel of seven microbes (aspergillus niger, bacillus cereus, candida albicans, escherichia coli, pseudomonas aeruginosa, saccharomyces cerevisiae and staphylococcus epidermidis) and employing the paper disc agar diffusion method. several aldehydes, most notably halogenate ... | 2007 | 17913062 |
| antimicrobial activity of two antitumour agents and ribonucleotide reductase inhibitors, pyridine-2-carboxaldehyde thiosemicarbazone and the acetate form of its copper(ii) chelate. | some copper chelates have potent antitumour activity, and in some cases also the free ligands have activity in vivo. yet, little is known about their antimicrobial properties. copper(ii) chelates of the thiosemicarbazones of a-n-heterocyclic carboxaldehydes constitute one important group of such agents, also their ligands having marked antitumour activity. both the ligands and chelates inhibit ribonucleotide reductase. some ligands have been or are under clinical trials as antineoplastic agents. ... | 2007 | 17913063 |
| antimicrobial activity of extracts of the lichen parmelia sulcata and its salazinic acid constituent. | the antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. all of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against aeromonas hydrophila, bacillus cereus, bacillus subtilis, listeria monocytogenes, proteus vulgaris, yersinia enterocolitica, staphylococcus aureus ... | 2007 | 17913083 |
| the zn2 position in metallo-beta-lactamases is critical for activity: a study on chimeric metal sites on a conserved protein scaffold. | metallo-beta-lactamases (mbetals) are bacterial zn(ii)-dependent hydrolases that confer broad-spectrum resistance to beta-lactam antibiotics. these enzymes can be subdivided into three subclasses (b1, b2 and b3) that differ in their metal binding sites and their characteristic tertiary structure. to date there are no clinically useful pan-mbetal inhibitors available, mainly due to the unawareness of key catalytic features common to all mbetal brands. here we have designed, expressed and characte ... | 2007 | 17915249 |
| surface reaction of bacillus cereus biomass and its biosorption for lead and copper ions. | in this study, the surface chemical functional groups of bacillus cereus biomass were identified by fourier transform infrared (ftir) analytical technique. it had been shown that the b. cereus cells mainly contained carboxyl, hydroxyl, phosphate, amino and amide functional groups. the potentiometric titration was conducted to explain the surface acid-base properties of aqueous b. cereus biomass. the computer program fiteql 4.0 was used to perform the model calculations. the optimization results ... | 2007 | 17915701 |
| effect of natural preservatives on the growth of histamine producing bacteria. | present study deals with the hampering of the growth of histamine producing bacteria (hpb), by using nacl and spices which are easily available and cheaper cost wise. for this experiment, four strains of hpb viz. vibrio parahaemolyticus, bacillus cereus, pseudomonas aeruginosa and proteus mirabilis were tested against 1 to 10% concentrations of nacl and 1 to 5% concentrations of natural preservatives (turmeric, ginger and garlic) in a basal medium. hpb showed different growth rates at different ... | 2007 | 17915763 |
| biosurfactant production and surface translocation are regulated by plcr in bacillus cereus atcc 14579 under low-nutrient conditions. | bacillus cereus atcc 14579 can respond to nutrient changes by adopting different forms of surface translocation. the b. cereus atcc 14579 deltaplcr mutant, but not the wild type, formed dendritic (branched) patterns on eps [a low-nutrient medium that contains 7.0 g k(2)hpo(4), 3.0 g kh(2)po(4), 0.1 g mgso(4).7h(2)o, 0.1 g (nh(4))(2)so(4), 0.01 g cacl(2), 0.001 g feso(4), 0.1 g nacl, 1.0 g glucose, and 125 mg yeast extract per liter] containing 0.7% agar. the dendritic patterns formed by sliding ... | 2007 | 17921286 |
| species-level identification of bacillus strains isolates from marine sediments by conventional biochemical, 16s rrna gene sequencing and inter-trna gene sequence lengths analysis. | the aim of this study was to compare the ability of commonly used conventional biochemical tests, sequencing analysis of 16s rrna genes and tdna-intergenic spacer length polymorphism (tdna-pcr) to identify species of the genus bacillus recovered from marine sediments. while biochemical tests were not sufficiently sensitive to distinguish between the 23 marine strains analyzed, partial 16s rrna gene sequences allowed a correct identification, clustering them into four species belonging to bacillu ... | 2008 | 17922298 |
| the inactivation and removal of airborne bacillus atrophaeus endospores from air circulation systems using uvc and hepa filters. | to (i) evaluate the uv radiation in the 'c' band/high efficient particulate air (uvc/hepa) instrument's potential to inactivate spores of bacillus atrophaeus and selected bacillus species and (ii) test whether a titanium dioxide coating inside the cylindrical hepa filter improves the system's efficacy. | 2008 | 17927759 |
| tentacle probes: differentiation of difficult single-nucleotide polymorphisms and deletions by presence or absence of a signal in real-time pcr. | false-positive results are a common problem in real-time pcr identification of dna sequences that differ from near neighbors by a single-nucleotide polymorphism (snp) or deletion. because of a lack of sufficient probe specificity, post-pcr analysis, such as a melting curve, is often required for mutation differentiation. | 2007 | 17932130 |
| proposal of a simplified technique for staining bacterial spores without applying heat--successful modification of moeller's method. | as the bacterial spores are difficult to stain, a number of staining techniques including their modifications have been proposed to date. most of the conventional staining procedures unexceptionally contain the step of staining with steamed dye reagent in order to increase the stainability of the spores. we made an attempt to improve the conventional moeller's methods for staining bacterial spores. | 2007 | 17933713 |
| cloning, expression and characterization of a fast self-sufficient p450: cyp102a5 from bacillus cereus. | cyp102s represent a family of natural self-sufficient fusions of cytochrome p450 and cytochrome p450 reductase found in some bacteria. one member of this family, named cyp102a1 or more traditionally p450bm-3, has been widely studied as a model of human p450 cytochromes. remarkable detail of p450 structure and function has been revealed using this highly efficient enzyme. the recent rapid expansion of microbial genome sequences has revealed many relatives of cyp102a1, but to date only two from ba ... | 2007 | 17945181 |
| discrimination of bacillus anthracis and closely related microorganisms by analysis of 16s and 23s rrna with oligonucleotide microarray. | analysis of 16s rrna sequences is a commonly used method for the identification and discrimination of microorganisms. however, the high similarity of 16s and 23s rrna sequences of bacillus cereus group organisms (up to 99-100%) and repeatedly failed attempts to develop molecular typing systems that would use dna sequences to discriminate between species within this group have resulted in several suggestions to consider b. cereus and b. thuringiensis, or these two species together with b. anthrac ... | 2008 | 17950718 |
| effects of low electric current (lec) treatment on pure bacterial cultures. | this research focused on the effects of low electric current (lec) on the cell viability and metabolic activity of escherichia coli and bacillus cereus. | 2007 | 17953548 |
| isolation and selection of bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish. | to isolate, select and evaluate bacillus spp. as potential biological agents for enhancement of water quality in culture of ornamental fish. | 2007 | 17953558 |
| effects of porcine bile on survival of bacillus cereus vegetative cells and haemolysin bl enterotoxin production in reconstituted human small intestine media. | to determine the effects of porcine bile (pb) on bacillus cereus vegetative cells and haemolysin bl (hbl) enterotoxin production in reconstituted small intestine media (im). | 2007 | 17953568 |
| characterization, n-terminal sequencing and classification of cerein mrx1, a novel bacteriocin purified from a newly isolated bacterium: bacillus cereus mrx1. | to purify and characterize the bacteriocin produced by strain mrx1. | 2007 | 17953573 |
| microwave-accelerated metal-enhanced fluorescence: application to detection of genomic and exosporium anthrax dna in <30 seconds. | we describe the ultra-fast and sensitive detection of the gene encoding the protective antigen of bacillus anthracis the causative agent of anthrax. our approach employs a highly novel platform technology, microwave-accelerated metal-enhanced fluorescence (mamef), which combines the use of metal-enhanced fluorescence to enhance assay sensitivity and focused microwave heating to spatially and kinetically accelerate dna hybridization. genomic and exosporium target dna of bacillus anthracis spores ... | 2007 | 17955147 |
| pseudochromhidrosis: blue discolouration of the head and neck. | a 57-year-old man is presented with blue pseudochromhidrosis affecting the face and neck following combination treatment with lansoprazole, a proton pump inhibitor, and ranitidine, a type two histamine receptor antagonist. the diagnosis was made on the basis of clinico-histological features and growth of malassezia furfur, and bacillus species, not bacillus cereus, in the absence of lipofuscin. the pseudochromhidrosis resolved on stopping both medications and did not recur on restarting only the ... | 2007 | 17956484 |
| culture-based bacterial detection systems for platelets: the effect of time prior to sampling and duration of incubation required for detection with aerobic culture. | bacterial contamination of platelet (plt) products occurs at low concentrations requiring a period of incubation for growth to minimize sampling error. culture-based detection methods also need sufficient incubation time; together these periods may limit the useful life of plts. this study characterizes the impact of sampling and detection times with two commercially available bacteria detection products. | 2007 | 17958533 |
| a multi-channel femtoampere-sensitivity conductometric array for biosensing applications. | rapid detection of pathogens using field deployable biosensors requires integrated sensing and data processing. detection of low concentration of biological agents is possible using accurate and real-time signal characterization devices. this paper presents a multi-channel conductometric array that can detect and measure current up to femtoampere range. the architecture uses a novel semi-synchronous sigmadelta modulation that allows measurement of ultra-small currents by using a hysteretic compa ... | 2006 | 17959433 |
| antibacterial effect of five zingiberaceae essential oils. | essential oil obtained by hydrodistillation and two different solvent extractions (petroleum ether and ethanol) from five zingiberaceae species: ginger (zingiber officinale roscoe.), galanga (alpinia galanga sw.), turmeric (curcuma longa l.), kaempferia (boesenbergia pandurata holtt.) and bastard cardamom (amomum xanthioides wall.) was characterized. volatile components of all extracts were analyzed by gas chromatography-mass spectrometry (gc-ms). the major components of ginger, turmeric, galang ... | 2007 | 17960105 |
| inhibitory effect of gram-negative and gram-positive microorganisms against helicobacter pylori clinical isolates. | to determine the in vitro inhibitory effect of several gram-negative and gram-positive microorganisms against helicobacter pylori clinical isolates. | 2008 | 17965421 |
| detection, isolation, and identification of cadmium-resistant bacteria based on pcr-dgge. | this study focused on the screening of cadmium-resistant bacterial strains from pb-zn tailing. we investigated the diversity of microbial community inhabiting dong-san-cha pb-zn tailing in beijing, china, by polymerase chain reaction-denaturing gradient gel electrophoresis (dgge) of 16s rrna gene of bacterial strain, and found two dominant strains in the dgge profile. using special culture media, we isolated two strong cadmium-resistant bacterial strains. on the basis of morphological, physiolog ... | 2007 | 17966518 |
| central venous catheter infection with bacillus pumilus in an immunocompetent child: a case report. | bacillus organisms are common laboratory contaminants. the majority of bacillus bacteraemias are transient and not clinically significant. clinically significant infection due to bacillus species is rare and mostly due to bacillus cereus infections in immuno-compromised hosts. | 2007 | 17967173 |
| efficacy of enterocin as-48 against bacilli in ready-to-eat vegetable soups and purees. | the broad-spectrum bacteriocin enterocin as-48 was tested for biopreservation of ready-to-eat vegetable foods (soups and purees) against aerobic mesophilic endospore-forming bacteria. by adding as-48 (10 microg/ml), bacillus cereus lwl1 was completely inhibited in all six vegetable products tested (natural vegetable cream, asparagus cream, traditional soup, homemade-style traditional soup, vegetable soup, and vichyssoise) for up to 30 days at 6, 15, and 22 degrees c. a collection of strains isol ... | 2007 | 17969616 |
| occurrence of bacillus cereus spores with a damaged exosporium: consequences on the spore adhesion on surfaces of food processing lines. | this study was designed to evaluate the occurrence of bacillus cereus spores with a damaged exosporium and the consequences of such damages on spore adhesion. the analysis of nine strains sporulated under optimal conditions (spo8-agar, 30 degrees c) revealed that damaged exosporia were systematically found in one strain (b. cereus d17) and occasionally in two others (b. cereus atcc 14579t and b. cereus d6). the prevalence of spores with damaged exosporia increased when sporulation occurred under ... | 2007 | 17969617 |
| [bacillus cereus pore-forming toxins hemolysin ii and cytotoxin k: polymorphism and distribution of genes among representatives of the cereus group]. | abstract-phylogenetic interrelation between 40 strains of the bacillus cereus group has been established using bcrep fingerprinting. the pcr method has shown that the frequency of occurrence of the genes of cytotoxin k (cytk) and hemolysin ii (hlyii) is 61% and 56%, respectively, and the gene of the hemolysin ii regulator (hlyiir) occurs together with hlyii. comparison of the results of fingerprinting, pcr, and rflp of the toxin genes showed that bacteria with the hlyii+ and cytk+ genotypes did ... | 2007 | 17974202 |
| exploring the evolution of the bacillus cereus group repeat element bcr1 by comparative genome analysis of closely related strains. | bcr1 is a chromosomal approximately 155 bp repeated element found uniquely and ubiquitously in the bacillus cereus group of gram-positive bacteria; it exhibits several features characteristic of mobile elements, including a variable distribution pattern between strains. here, highly similar bcr1 elements in non-conserved genomic loci are identified in a set of closely related b. cereus and bacillus thuringiensis strains near the bacillus anthracis phylogenetic cluster. it is also shown that bcr1 ... | 2007 | 17975097 |
| cell wall carbohydrate compositions of strains from the bacillus cereus group of species correlate with phylogenetic relatedness. | members of the bacillus cereus group contain cell wall carbohydrates that vary in their glycosyl compositions. recent multilocus sequence typing (mlst) refined the relatedness of b. cereus group members by separating them into clades and lineages. based on mlst, we selected several b. anthracis, b. cereus, and b. thuringiensis strains and compared their cell wall carbohydrates. the cell walls of different b. anthracis strains (clade 1/anthracis) were composed of glucose (glc), galactose (gal), n ... | 2008 | 17981984 |
| supercat: a supertree database for combined and integrative multilocus sequence typing analysis of the bacillus cereus group of bacteria (including b. cereus, b. anthracis and b. thuringiensis). | the bacillus cereus group of bacteria is an important group including mammalian and insect pathogens, such as b. anthracis, the anthrax bacterium, b. thuringiensis, used as a biological pesticide and b. cereus, often involved in food poisoning incidents. to characterize the population structure and epidemiology of these bacteria, five separate multilocus sequence typing (mlst) schemes have been developed, which makes results difficult to compare. therefore, we have developed a database that comp ... | 2008 | 17982177 |
| sphingomyelinase generation of ceramide promotes clustering of nanoscale domains in supported bilayer membranes. | the effects of ceramide incorporation in supported bilayers prepared from ternary lipid mixtures which have small nanoscale domains have been examined using atomic force and fluorescence microscopy. both direct ceramide incorporation in vesicles used to prepare the supported bilayers and enzymatic hydrolysis of sm by sphingomyelinase were compared for membranes prepared from 5:5:1 dopc/sphingomyelin/cholesterol mixtures. both methods of ceramide incorporation resulted in enlargement of the initi ... | 2008 | 17988649 |
| [effects of rhamnolipid on the biodegradation of n-hexadecane by microorganism and the cell surface hydrophobicity]. | the experiments were made in laboratory to analyze the effects of rhamnolipid on the biodegradation of n-hexadecane and the cell surface hydrophobicity using the microorganism ( bacillus cereus dq01 and bacillus sp. dq02). the results show that the optimal concentrations range of rhamnolipid were 0.4 mmol/l and 0.2 mmol/l, respectively, which can obviously enhance bath(bacterial adherence to hydrocarbon). the degradation of n-hexadecane by bacillus cereus dq01 and bacillus sp. dq02 were increase ... | 2007 | 17990568 |
| metabolic capacity of bacillus cereus strains atcc 14579 and atcc 10987 interlinked with comparative genomics. | bacillus cereus is an important food-borne pathogen and spoilage organism. in this study, numerous phenotypes and the genomes of b. cereus strains atcc 14579 and atcc 10987 were analysed to compare their metabolic capacity and stress resistance potential. the growth performance of the two strains was assessed for nearly 2000 phenotypes, including use of nutrient sources, performance in acid and basic environments, osmo-tolerance and antibiotic resistance. several food-relevant phenotypic differe ... | 2007 | 17991024 |
| influence of the sporulation temperature on the impact of the nutrients inosine and l-alanine on bacillus cereus spore germination. | the effect of sporulation temperature on bacillus cereus spore germination triggered by the nutrient germinants inosine and l-alanine was investigated. the germination (expressed as % a(600nm) fall) of heat-activated spores of b. cereus strain atcc14579 produced at 20 and 37 degrees c, and of psychrotrophic strains lm9 and d15 produced at 15 and 37 degrees c was followed in a germination buffer containing inosine at concentrations between 0.01 and 10mmoll(-1), or l-alanine between 1 and 100mmoll ... | 2008 | 17993396 |
| cgas: a comparative genome annotation system. | recent advances in genome sequencing technology and algorithms have made it possible to determine the sequence of a whole genome quickly in a cost-effective manner. as a result, there are more than 200 completely sequenced genomes. however, annotation of a genome is still a challenging task. one of the most effective methods to annotate a newly sequenced genome is to compare it with well-annotated and closely related genomes using computational tools and databases. comparing genomes requires use ... | 2007 | 17993671 |
| development of a microbial population within a hot-drinks vending machine and the microbial load of vended hot chocolate drink. | in order to understand the development of the microbial population within a hot-drinks vending machine a new machine was placed in a staff area of a university campus vending only hot chocolate. the machine was cleaned weekly using a detergent based protocol. samples from the mixing bowl, dispense area, and drink were taken over a 19-wk period and enumerated using plate count agar. bacillus cereus was identified using biochemical methods. vended drinks were sampled at 0, 3, 6, and 9 min after ve ... | 2007 | 17995650 |
| recipes for antimicrobial wine marinades against bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, and salmonella enterica. | we have evaluated bactericidal activities against bacillus cereus, escherichia coli o157:h7, listeria monocytogenes, and salmonella enterica of several antimicrobial wine recipes, each consisting of red or white wine extracts of oregano leaves with added garlic juice and oregano oil. dose-response plots were used to determine the percentage of the recipes that resulted in a 50% decrease in colony-forming units (cfu) at 60 min (ba(50)). studies designed to optimize antibacterial activities of the ... | 2007 | 17995688 |
| genetic distribution of 295 bacillus cereus group members based on adk-screening in combination with mlst (multilocus sequence typing) used for validating a primer targeting a chromosomal locus in b. anthracis. | the genetic distribution of 295 bacillus cereus group members has been investigated by using a modified multilocus sequence typing method (mlst). by comparing the nucleic acid sequence of the adk gene fragment, isolates of b. cereus group members most related to b. anthracis may be easily identified. the genetic distribution, with focus on the b. anthracis close neighbours, was used to evaluate a new primer set for specific identification of b. anthracis. this primer set, ba5510-1/2, targeted th ... | 2007 | 17997177 |
| structure of plcr: insights into virulence regulation and evolution of quorum sensing in gram-positive bacteria. | gram-positive bacteria use a wealth of extracellular signaling peptides, so-called autoinducers, to regulate gene expression according to population densities. these "quorum sensing" systems control vital processes such as virulence, sporulation, and gene transfer. using x-ray analysis, we determined the structure of plcr, the major virulence regulator of the bacillus cereus group, and obtained mechanistic insights into the effects of autoinducer binding. our structural and phylogenetic analysis ... | 2007 | 17998541 |
| bioactivity and chemical composition of the leaf essential oils of zanthoxylum rhoifolium and zanthoxylum setulosum from monteverde, costa rica. | the leaf essential oils of zanthoxylum rhoifolium and zanthoxylum setulosum (rutaceae) from monteverde, costa rica have been obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry. the principal constituents of z. rhoifolium leaf oil were germacrene d (14.6%), limonene (12.5%), trans-2-hexenal (11.3%), beta-elemene (9.2%), 2-undecanone (9.2%), myrcene (7.9%), bicyclogermacrene (7.5%), and germacrene a (5.2%). the leaf oil of z. setulosum was composed largely of beta-p ... | 2008 | 17999336 |
| [investigation of a foodborne intoxication in a high-density penitentiary center]. | on september 25 2005, more than 100 inmates (almost 5% of the 1,800 prison population) experienced sudden onset gastroenteritis. this outbreak was the largest foodborne outbreak described in a prison population in spain. our objective was to confirm the outbreak, identify risk factors, implement control measures, and provide recommendations. | 2007 | 18001657 |
| bacillus cereus in free-stall bedding. | to increase the understanding of how different factors affect the bacterial growth in deep sawdust beds for dairy cattle, the microbiological status of bacillus cereus and coliforms in deep sawdust-bedded free stalls was investigated over two 14-d periods on one farm. high counts of b. cereus and coliforms were found in the entire beds. on average, 4.1 log(10) b. cereus spores, 5.5 log(10) b. cereus, and 6.7 log(10) coliforms per gram of bedding could be found in the upper layers of the sawdust ... | 2007 | 18024738 |
| production of nisin z by lactococcus lactis isolated from dahi. | lactococcus lactis cm1, an isolate from homemade "dahi," a traditional fermented milk from india, used maltose as carbon source to produce a high level of bacteriocin. the bacterial cell mass and the bacteriocin production correlated with the initial ph of the medium and were highest when the initial ph was 11.0. the level of bacteriocin reached its peak at the late log phase with concomitant reduction of culture ph to 4.2, regardless of the initial ph of the medium. a combination of maltose and ... | 2007 | 18025595 |
| potential and limitation of uvc irradiation for the inactivation of pathogens in platelet concentrates. | pathogen contamination, causing transfusion-transmitted diseases, is an ongoing concern in transfusion of cellular blood products. in this explorative study, the pathogen-inactivating capacity of uvc irradiation in platelet (plt) concentrates was investigated. the dose dependencies of inactivation of several viruses and bacteria were compared with the effect on plt quality. | 2008 | 18028277 |
| identification and expression of gh-8 family chitosanases from several bacillus thuringiensis subspecies. | the gram-positive spore-forming bacterium, bacillus thuringiensis, a member of the bacillus cereus group, produces chitosanases that catalyze the hydrolysis of chitosan to chitosan-oligosaccharides (cos). although fungal and bacterial chitosanases belonging to other glycoside hydrolase (gh) families have been characterized in a variety of microorganisms, knowledge on the genetics and phylogeny of the gh-8 chitosanases remains limited. nine genes encoding chitosanases were cloned from 29 differen ... | 2007 | 18031332 |
| unconventional giy-yig homing endonuclease encoded in group i introns in closely related strains of the bacillus cereus group. | several group i introns have been previously found in strains of the bacillus cereus group at three different insertion sites in the nrde gene of the essential nrdief operon coding for ribonucleotide reductase. here, we identify an uncharacterized group ia intron in the nrdf gene in 12 strains of the b. cereus group and show that the pre-mrna is efficiently spliced. the bacillus thuringiensis ssp. pakistani nrdf intron encodes a homing endonuclease, denoted i-bthii, with an unconventional giy-(x ... | 2008 | 18032435 |
| establishment and characterization of dual-species biofilms formed from a 3,5-dinitrobenzoic-degrading strain and bacteria with high biofilm-forming capabilities. | in this study, the possibility of establishing a dual-species biofilm from a bacterium with a high biofilm-forming capability and a 3,5-dinitrobenzoic acid (3,5-dnba)-degrading bacterium, comamonas testosteroni a3, was investigated. our results showed that the combinations of strain a3 with each of five strains with a high biofilm-forming capability (pseudomonas sp. m8, pseudomonas putida m9, bacillus cereus m19, pseudomonas plecoglossicida m21 and aeromonas hydrophila m22) presented different l ... | 2008 | 18034834 |
| ecological diversification in the bacillus cereus group. | the bacillus cereus group comprises organisms that are widely distributed in the environment and are of health and economic interest. we demonstrate an 'ecotypic' structure of populations in the b. cereus group using (i) molecular data from fluorescent amplified fragment length polymorphism patterns, ribosomal gene sequences, partial panc gene sequences, 'psychrotolerant' dna sequence signatures and (ii) phenotypic and descriptive data from range of growth temperature, psychrotolerance and therm ... | 2008 | 18036180 |
| [bacillus cereus is a microbial decomposer of 2,4-dichlorophenol]. | a microorganism degrading 2,4-dichlorophenol isolated from an aeration pond of the baikal pulp and paper mill was identified as bacillus cereus bip507 based on morphological and physiological characters as well as 16s rdna sequencing. this microorganism proved able to degrade high 2,4-dichlorophenol concentrations (up to 560 microm). | 2007 | 18038618 |
| antibacterial activity of guava (psidium guajava l.) and neem (azadirachta indica a. juss.) extracts against foodborne pathogens and spoilage bacteria. | the antibacterial activity of guava (psidium guajava) and neem (azadirachta indica) extracts against 21 strains of foodborne pathogens were determined--listeria monocytogenes (five strains), staphylococcus aureus (four strains), escherichia coli o157:h7 (six strains), salmonella enteritidis (four strains), vibrio parahaemolyticus, and bacillus cereus, and five food spoilage bacteria: pseudomonas aeroginosa, p. putida, alcaligenes faecalis, and aeromonas hydrophila (two strains). guava and neem e ... | 2007 | 18041957 |
| inhibition of bacillus cereus in milk fermented with kefir grains. | the effects of kefir-fermented milk were tested against a toxigenic strain of bacillus cereus. the incubation of milk with b. cereus spores plus 5% kefir grains prevented spore germination and growth of vegetative forms. in contrast, when 1% kefir grains was used, no effects were observed. the presence of metabolically active kefir grains diminished titers of nonhemolytic enterotoxin a, as assessed by enzyme-linked immunosorbent assay. during fermentation, kefir microorganisms produce extracellu ... | 2007 | 18044443 |
| characterization of peptide deformylase2 from b. cereus. | peptide deformylase (pdf) is a metalloenzyme that removes the n-terminal formyl groups from newly synthesized proteins. it is essential for bacterial survival, and is therefore-considered as a potential target for antimicrobial chemotherapy. however, some bacteria including medically relevant pathogens possess two or more def-like genes. here we have examined two pdfs from bacillus cereus. the two share only 32% sequence identity and the crystal structures show overall similarity with pdf2 havin ... | 2007 | 18047803 |
| generation of a specific marker to discriminate gacillus anthracis from other bacteria of the bacillus cereus group. | bacillus anthracis is a soil pathogen capable of causing anthrax that is closely related to several environmental species, including b. cereus, b. mycoides, and b. thuringiensis. dna homology studies showed that b. anthracis, b. cereus, b. mycoides, and b. thuringiensis are closely related, with a high sequence homology. to establish a method to specifically detect b. anthracis in situations such as environmental contamination, we initially performed rapd-pcr with a 10-mer random primer and conf ... | 2007 | 18051303 |
| simultaneous detection and identification of bacillus cereus group bacteria using multiplex pcr. | bacillus cereus group bacteria share a significant degree of genetic similarity. thus, to differentiate and identify the bacillus cereus group efficiently, a multiplex pcr method using the gyrb and groel genes as diagnostic markers is suggested for simultaneous detection. the assay yielded a 400 bp amplicon for the groel gene from all the b. cereus group bacteria, and a 253 bp amplicon from b. anthracis, 475 bp amplicon from b. cereus, 299 bp amplicon from b. thuringiensis, and 604 bp amplicon f ... | 2007 | 18051330 |
| crystallization and preliminary x-ray diffraction analysis of bcomt2 from bacillus cereus: a family of o-methyltransferase. | o-methyltransferases (omts), one of the ubiquitous enzymes in plants, bacteria, and humans, catalyze a methyl-transfer reaction using s-adenosylmethionine and a wide range of phenolics as a methyl donor and acceptor, respectively. substrates for most bacterial omts have largely remained elusive, but recent investigation using bcomt2, an omt from bacillus cereus, suggested that ortho-dihydroxyflavonoids could serve as substrates. to elucidate the functional and structural features of bcomt2, we e ... | 2007 | 18051771 |
| evaluation of standard and new chromogenic selective plating media for isolation and identification of bacillus cereus. | in this study, the performance of two new chromogenic plating media (cbc and bcm) was compared with two standard selective plating media (pemba and myp) recommended by food authorities for isolation, identification and enumeration of bacillus cereus. the four media types were challenged with a strain set comprising 100 b. cereus isolates from different origins and with different toxigenic potentials (40 food isolates, 40 isolates from food borne outbreaks and 20 clinical isolates). additionally, ... | 2008 | 18055052 |
| detection of bacillus cereus virulence factors in commercial products of bacillus thuringiensis and expression of diarrheal enterotoxins in a target insect. | we examined isolates from 4 commercial bioinsecticides based on different strains of bacillus thuringiensis subspecies (kurstaki, israelensis, aizawai, and tenebrionis) for the presence of genes encoding proteins with known enterotoxigenicity (nhe, hbl, cytk, ces) and various other putative virulence genes (piplc, sph, bcet, entfm, ents, entt). the piplc and bcet sequences were present in all the isolates; sph was found in aizawai and israelensis; entfm only in israelensis; and ents in kurstaki, ... | 2007 | 18059560 |
| biological activity of phenylpropionic acid isolated from a terrestrial streptomycetes. | the strain anu 6277 was isolated from laterite soil and identified as streptomyces sp. closely related to streptomyces albidoflavus cluster by 16s rrna analysis. the cultural, morphological and physiological characters of the strain were recorded. the strain exhibited resistance to chloramphenicol, penicillin and streptomycin. it had the ability to produce enzymes such as amylase and chitinase. a bioactive compound was isolated from the strain at stationary phase of culture and identified as 3-p ... | 2007 | 18062653 |
| identification of beta-lactamase in antibiotic-resistant bacillus cereus spores. | beta-lactamase type i is reported for the first time to occur in the sporulated form in a penicillin-resistant bacillus species. the enzyme was readily characterized from the b. cereus 5/b line (atcc 13061) by mass spectrometry and two-dimensional gel electrophoresis. | 2008 | 18065609 |
| inhibition of listeria monocytogenes in dairy products using the bacteriocin-like peptide cerein 8a. | the efficacy of the antimicrobial peptide cerein 8a to control the development of listeria monocytogenes in milk and soft cheese was investigated. the addition of 160 au ml(-1) cerein 8a to uht milk resulted in a decrease of 3 log cycles in viable cells within the 14-day period at 4 degrees c. the viable counts of l. monocytogenes in pasteurized milk samples containing cerein 8a was lower than those observed in controls without bacteriocin. addition of cerein 8a to minas-type soft cheese caused ... | 2008 | 18068253 |
| human chitotriosidase is expressed in the eye and lacrimal gland and has an antimicrobial spectrum different from lysozyme. | chitotriosidase is a chitinolytic enzyme expressed by maturing macrophages and preformed in neutrophil granules, suggesting a role in antimicrobial defence. although available evidence supports a role in anti-fungal immunity, there is a lack of an obvious phenotype in humans homozygous for a mutation which renders chitotriosidase inactive. this may be explained by compensatory effects of enzymes co-expressed with chitotriosidase, such as lysozyme. we have found that chitinase is highly expressed ... | 2008 | 18068392 |
| broad distribution of enterotoxin genes (hblcda, nheabc, cytk, and entfm) among bacillus thuringiensis and bacillus cereus as shown by novel primers. | eight new pairs of pcr primers were designed and efficiently detect eight toxin genes (hblc, hbld, hbla, nhea, nheb, nhec, cytk, and entfm) in 411 b. cereus strains (121 food- and 290 soil isolates) and 205 b. thuringiensis strains (43 serovars, 10 food- and 152 soil isolates). according to the presence of these eight toxin genes, they were divided into four groups among the total 616 isolates. in group i, all eight genes occurred simultaneously in 403 (65.42%) isolates, while group ii (134 isol ... | 2008 | 18068844 |
| genetic and biochemical characterization of cad-1, a chromosomally encoded new class a penicillinase from carnobacterium divergens. | carnobacterium divergens clinical isolates bm4489 and bm4490 were resistant to penicillins but remained susceptible to combinations of amoxicillin-clavulanic acid and piperacillin-tazobactam. cloning and sequencing of the responsible determinant from bm4489 revealed a coding sequence of 912 bp encoding a class a beta-lactamase named cad-1. the bla(cad-1) gene was assigned to a chromosomal location in the two strains that had distinct pulsed-field gel electrophoresis patterns. cad-1 shared 53% an ... | 2008 | 18070972 |
| the occurrence of bacillus cereus, b. thuringiensis and b. mycoides in chinese pasteurized full fat milk. | in 2006, a total of 54 samples of pasteurized full fat milk packaged in cartons were collected in spring and in autumn from chain supermarkets in wuhan, china. the samples were examined and enumerated by mpn methods strictly according to guidelines laid out in us fda/cfsan bam chapter 14. among 102 isolated b. cereus-like bacteria, 92 isolates were identified to be b. cereus, 9 b. thuringiensis and 1 b. mycoides. it was found that the occurrences of b. cereus were 71.4% and 33.3% in spring and i ... | 2008 | 18077041 |
| hydrogen and polyhydroxybutyrate producing abilities of microbes from diverse habitats by dark fermentative process. | thirty five bacterial isolates from diverse environmental sources such as contaminated food, nitrogen rich soil, activated sludges from pesticide and oil refineries effluent treatment plants were found to belong to bacillus, bordetella, enterobacter, proteus, and pseudomonas sp. on the basis of 16s rrna gene sequence analysis. under dark fermentative conditions, maximum hydrogen (h(2)) yields (mol/mol of glucose added) were recorded to be 0.68 with enterobacter aerogenes egu16 followed by 0.63 w ... | 2008 | 18083024 |
| multiple-locus sequence typing and analysis of toxin genes in bacillus cereus food-borne isolates. | in the present study we characterized 47 food-borne isolates of bacillus cereus using multilocus sequence typing (mlst). newly determined sequences were combined with sequences available in public data banks in order to produce the largest data set possible. phylogenetic analysis was performed on a total of 296 strains for which mlst sequence information is available, and three main lineages--i, ii, and iii--within the b. cereus complex were identified. with few exceptions, all food-borne isolat ... | 2008 | 18083872 |
| [emergence of beta-lactam-dependent bacillus cereus associated with prolonged treatment with cefepime in a neutropenic patient]. | antibiotic dependence in clinical isolates has been reported, albeit rarely, such as vancomycin-dependent enterococcus and beta-lactam-dependent staphylococcus saprophyticus. we report herein a clinical isolate of beta-lactam-dependent bacillus cereus. a 16-yr-old female was admitted on 8 september 2005 with neutropenic fever during chemotherapy following surgical removal of peripheral neuroectodermal tumor. she had had an indwelling chemoport since august 2004 and experienced b. cereus bacterem ... | 2007 | 18094579 |
| development of rapid real-time pcr and most-probable-number real-time pcr assays to quantify enterotoxigenic strains of the species in the bacillus cereus group. | members of the bacillus cereus group may produce diarrheal enterotoxins and could be potential hazards if they enter the food chain. therefore, a method capable of detecting all the species in the b. cereus group rather than b. cereus alone is important. we selected nhe as the target and developed a real-time pcr assay to quantify enterotoxigenic strains of the b. cereus group. the real-time pcr assay was evaluated with 60 b. cereus group strains and 28 others. the assay was also used to constru ... | 2007 | 18095430 |
| isolation and characterization of a psychrotolerant toxin producer, bacillus weihenstephanensis, in liquid egg products. | a psychrotolerant bacteria of the bacillus cereus group was found responsible for the spoilage of whole liquid egg products. by sequencing a 16s rrna region and performing a pcr amplification of specific 16s rrna and cspa signatures, a bacillus weihenstephanensis was identified. characterization of this strain shows its ability to grow in defined medium as well as in whole liquid egg at refrigerated temperatures. the strain isolated possesses genes encoding for hemolysin bl, nonhemolytic enterot ... | 2007 | 18095431 |
| bacterial contamination of sago starch in papua new guinea. | sago starch is an important food in lowland papua new guinea. extraction of the starch from the palm and storage were performed by way of traditional methods that have been used for thousands of years. currently, very little is known about the microbiology of sago starch. sago samples were collected from areas of high starch utilization and analyzed for the presence of bacterial pathogens and indicator organisms. storage methods and duration were recorded at the time of collection, and ph and wa ... | 2007 | 18095446 |
| cleaning and handling implements as potential reservoirs for bacterial contamination of some ready-to-eat foods in retail delicatessen environments. | this study assessed the association of bacteria with cleaning tools, such as floor mops (n = 25) and cleaning cloths (n = 39), and handling devices, such as disposable plastic gloves (n = 20), used during filled baguette and assorted salad preparation in four selected retail delicatessens in johannesburg, south africa. samples of each cleaning or handling tool were prepared for aerobic (apc), coliform (cc), escherichia coli (ec), bacillus cereus (bcc), and staphylococcus aureus (sac) counts, as ... | 2007 | 18095448 |
| expression of functional bacillus spoiisab toxin-antitoxin modules in escherichia coli. | spoiisa and spoiisb proteins from bacillus subtilis belong to a recently described bacterial programmed-cell death system. the current work demonstrates that the toxin-antitoxin module is also functional in escherichia coli cells, where the expression of spoiisa toxin leads to transient growth arrest coupled with cell lysis, and spoiisa-induced death can be prevented by coexpression of its cognate antitoxin, spoiisb. escherichia coli cells appear to be able to escape the spoiisa killing by activ ... | 2008 | 18096016 |
| biocerin; an antibiotic produced by bacillus cereus. | | 1949 | 18108882 |
| the lecithinase of bacillus cereus and its comparison with clostridium welchii alpha-toxin. | | 1949 | 18149939 |
| biochemical factors influencing sporulation in a strain of bacillus cereus. | | 1949 | 18150950 |
| the genetically remote pathogenic strain nvh391-98 of the bacillus cereus group is representative of a cluster of thermophilic strains. | bacteria of the bacillus cereus group are known to cause food poisoning. a rare phylogenetically remote strain, nvh391-98, was recently characterized to encode a particularly efficient cytotoxin k presumably responsible for food poisoning. this pathogenic strain and its close relatives can be phenotypically distinguished from other strains of the b. cereus group by the inability to grow at temperatures below 17 degrees c and by the ability to grow at temperatures from 48 to 53 degrees c. a tempe ... | 2008 | 18156332 |
| [key original articles published in the "annales françaises d'anesthésie et de réanimation" (years 2006-2007)]. | | 2008 | 18166290 |
| kefiran antagonizes cytopathic effects of bacillus cereus extracellular factors. | kefiran, the polysaccharide produced by microorganisms present in kefir grains, is a water-soluble branched glucogalactan containing equal amounts of d-glucose and d-galactose. in this study, the effect of kefiran on the biological activity of bacillus cereus strain b10502 extracellular factors was assessed by using cultured human enterocytes (caco-2 cells) and human erythrocytes. in the presence of kefiran concentrations ranging from 300 to 1000 mg/l, the ability of b. cereus b10502 spent cultu ... | 2008 | 18174111 |
| x-ray crystal structure of the b component of hemolysin bl from bacillus cereus. | bacillus cereus hemolysin bl enterotoxin, a ternary complex of three proteins, is the causative agent of food poisoning and requires all three components for virulence. the x-ray structure of the binding domain of hbl suggests that it may form a pore similar to other soluble channel forming proteins. a putative pathway of pore formation is discussed. | 2008 | 18175317 |
| [microbiological monitoring of milk samples and surface samples in a hospital infant formula room]. | analyze the microbiological quality of reconstituted milk formulas and the surface hygiene in a hospital infant formula room, in the context of a worldwide emergence of enterobacter sakazakii infections. | 2008 | 18178021 |
| detection of large plasmids from the bacillus cereus group. | the members of the bacillus cereus group, bacillus anthracis, bacillus thuringiensis, and b. cereus senso stricto, are largely defined by their content of large plasmids, which encode major virulence factors. here we offer an easy, fast, and reliable protocol for the isolation and detection of large plasmids up to the size of at least 350kb. furthermore, using this method, we report that bacillus mycoides contain large plasmids. | 2008 | 18179822 |
| comparative analysis of polyphenolic profiles and antioxidant and antimicrobial activities of tunisian pome fruit pulp and peel aqueous acetone extracts. | pome trees, apple, pear, and quince, are classified into the subfamily pomoideae, belonging to the rosaceae family. their autumnal fruits are consumed worldwide in different forms, that is, fresh or transformed into jams, jelly, juices, etc. their well-established beneficial properties to human health were found mainly related to their phenolic content. pulp and peel aqueous acetone extracts obtained from tunisian fruits at commercial maturity were comparatively evaluated for their phenolic prof ... | 2008 | 18181568 |
| high-performance liquid chromatographic assay with ultraviolet spectrometric detection for the evaluation of inhibitors of phosphatidylinositol-specific phospholipase c. | a nonradioactive spectrometric assay for the evaluation of inhibitors of phosphatidylinositol-specific phospholipase c (pi-plc) is described. l-alpha-phosphatidylinositol from bovine liver was used as substrate in the presence of the micelle-forming detergent deoxycholic acid. pi-plc isolated from bacillus cereus and crude cytosol fractions from porcine brain were used as enzyme sources. pi-plc activity was determined by measuring the release of 1-stearoyl-2-arachidonoyl-sn-glycerol with reverse ... | 2008 | 18191462 |
| polyhydroxyalkanoate biosynthesis in bacillus cereus spv under varied limiting conditions and an insight into the biosynthetic genes involved. | a new strain of bacillus, bacillus cereus spv, was found to be capable of using a wide range of carbon sources for the production of polyhydroxyalkanoates (phas) (valappil et al. 2007b). limiting nutrient in the culture conditions is crucial for pha production. in this study, b. cereus spv was grown in different culture conditions with limitation of potassium, nitrogen, sulphur and phosphorous to establish the impact of nutritional limitation on pha production. | 2008 | 18194257 |
| polymeric glycoconjugates protect and activate macrophages to promote killing of bacillus cereus spores during phagocytosis. | diseases caused by bacillus spores might be attenuated if macrophages were able to kill the spores on exposure. glycoconjugate-bearing polymers, which have been shown to bind to bacillus spores, were tested for modulation of phagocytosis of b. cereus spores. without glycoconjugate activation, murine macrophages were ineffective at killing bacillus spores during phagocytosis. in the presence of glycoconjugates, however, the macrophages efficiently killed the organisms. the glycoconjugates were sh ... | 2008 | 18202914 |
| inactivation of microbial arginine deiminases by l-canavanine. | arginine deiminase (adi) catalyzes the hydrolytic conversion of l-arginine to ammonia and l-citrulline as part of the energy-producing l-arginine degradation pathway. the chemical mechanism for adi catalysis involves initial formation and subsequent hydrolysis of a cys-alkylthiouronium ion intermediate. the structure of the pseudomonas aeruginosa adi-(l-arginine) complex guided the design of arginine analogs that might react with the adis to form inactive covalent adducts during catalytic turnov ... | 2008 | 18205354 |
| bacillus cereus endospores exhibit a heterogeneous response to heat treatment and low-temperature storage. | bacillus cereus endospores were challenged by heat treatments simulating typical domestic/industrial cooking regimes and the resulting effects on germination, viability and sub-lethal heat damage determined using differential plate counting on a rich versus selective medium, flow cytometry (fcm), beta-d-glucuronidase (gud) activity and od(600) measurement. additionally, these techniques were used to investigate the effect on endospores of storage in a non-nutrient medium at 4 degrees c for 1 mon ... | 2008 | 18206765 |
| succession of dominant and antagonistic lactic acid bacteria in fermented cucumber: insights from a pcr-based approach. | the goal of the investigation was to study the succession of major groups of lactic acid bacteria (lab) and their antagonism in salt-fermented cucumber using pcr. in a direct detection method as well as a short enrichment process, pcr enabled detection of leuconostoc and lactobacillus during early hours of fermentation. subsequently, lactobacillus and pediococcus emerged as the dominant genera. nucleic acid sequence of culture-independent clones confirmed the detection of pediococcus as a domina ... | 2008 | 18206770 |
| application of gaseous ozone to control populations of escherichia coli, bacillus cereus and bacillus cereus spores in dried figs. | the effect of ozonation as a method to reduce escherichia coli, bacillus cereus and bacillus cereus spores in dried figs was investigated. dried figs were sprinkle inoculated with e. coli, b. cereus and b. cereus spores in sterile bags at a level of 10(7)microorganism g(-1), mixed and allowed to dry for 1h at 25 degrees c prior to ozonation. inoculated samples were exposed to gaseous ozone in a chamber at 20 degrees c and 70% relative humidity. ozone concentrations of 0.1, 0.5 and 1.0 ppm up to ... | 2008 | 18206781 |
| high incidence of plant growth-stimulating bacteria associated with the rhizosphere of wheat grown on salinated soil in uzbekistan. | soil salinization is increasing steadily in many parts of the world and causes major problems for plant productivity. under these stress conditions, root-associated beneficial bacteria can help improve plant growth and nutrition. in this study, salt-tolerant bacteria from the rhizosphere of uzbek wheat with potentially beneficial traits were isolated and characterized. eight strains which initially positively affect the growth of wheat plants in vitro were investigated in detail. all eight strai ... | 2008 | 18211262 |
| characterization of the cody gene and its influence on biofilm formation in bacillus cereus. | the foodborne pathogen bacillus cereus can form biofilms on various food contact surfaces, leading to contamination of food products. to study the mechanisms of biofilm formation by b. cereus, a tn5401 library was generated from strain uw101c. eight thousand mutants were screened in eps, a low nutrient medium. one mutant (m124), with a disruption in cody, developed fourfold less biofilm than the wild-type, and its defective biofilm phenotype was rescued by complementation. addition of 0.1% casam ... | 2008 | 18214442 |
| a single amino acid change is responsible for evolution of acyltransferase specificity in bacterial methionine biosynthesis. | bacteria and yeast rely on either homoserine transsuccinylase (hts, meta) or homoserine transacetylase (hta; met2) for the biosynthesis of methionine. although hts and hta catalyze similar chemical reactions, these proteins are typically unrelated in both sequence and three-dimensional structure. here we present the 2.0 a resolution x-ray crystal structure of the bacillus cereus meta protein in complex with homoserine, which provides the first view of a ligand bound to either hta or hts. surpris ... | 2008 | 18216013 |
| molecular characterization of virulence defects in bacillus thuringiensis mutants. | sequence analysis of a virulence-attenuated bacillus thuringiensis signature tagged mutant 6f8 led to the identification of an 18 182 bp locus encoding 29 potential protein-coding orfs. thirteen of the 29 putative orfs were found to share extensive homology with genes on plasmid pe33l466 of the pathogenic bacillus cereus e33l strain. nine orfs were not only found in a cluster, but also in the same gene order, in both organisms. a number of mobile elements, including a transposon tn4430, a novel ... | 2008 | 18218018 |
| petrobactin-mediated iron transport in pathogenic bacteria: coordination chemistry of an unusual 3,4-catecholate/citrate siderophore. | | 2008 | 18220393 |
| synergistic antibacterial activity between thymus vulgaris and pimpinella anisum essential oils and methanol extracts. | essential oils (eos) and methanol extracts obtained from aerial parts of thymus vulgaris and pimpinella anisum seeds were evaluated for their single and combined antibacterial activities against nine gram-positive and gram-negative pathogenic bacteria: staphylococcus aureus, bacillus cereus, escherichia coli, proteus vulgaris, proteus mirabilis, salmonella typhi, salmonella typhimurium, klebsiella pneumoniae and pseudomonas aeruginosa. the essential oils and methanol extracts revealed promising ... | 2008 | 18226481 |