| epidemiology and microbiology of secondary peritonitis caused by viscus perforation: a single-center retrospective study. | complicated intra-abdominal infections are serious conditions that require urgent source control and antibiotic treatment. the purpose of this study was to evaluate the epidemiology and bacterial causation of such infections using blood and peritoneal cultures of korean patients with peritonitis originating from viscus perforation. | 2015 | 26061903 |
| nosocomial infections and microbiologic spectrum after major elective surgery of the pancreas, liver, stomach, and esophagus. | the majority of infections treated by surgeons are nosocomial infections (ni). the frequency of these infections in relation to the organ operated on as well as the organisms involved are not well defined. detailed knowledge of these issues is essential for optimal care of surgical patients. this study aimed to determine infection rates and the responsible pathogens after major elective surgery of the pancreas, liver, stomach, and esophagus. | 2015 | 26046248 |
| development of a dry inoculation method for thermal challenge studies in low-moisture foods by using talc as a carrier for salmonella and a surrogate (enterococcus faecium). | the objective of this study was to obtain dry inocula of salmonella tennessee and enterococcus faecium, a surrogate for thermal inactivation of salmonella in low-moisture foods, and to compare their thermal resistance and stability over time in terms of survival. two methods of cell growth were compared: cells harvested from a lawn on tryptic soy agar (tsa-cells) and from tryptic soy broth (tsb-cells). concentrated cultures of each organism were inoculated onto talc powder, incubated at 35 °c fo ... | 2015 | 26038899 |
| isolation and biochemical fingerprinting of vancomycin-resistant enterococcus faecium from meat, chicken and cheese. | vancomycin-resistant enterococci (vre) are important nosocomial pathogens and food chain has been considered as an assumed source for dissemination of vre to human. | 2015 | 26034532 |
| multiple hospital outbreaks of vana enterococcus faecium in denmark, 2012-13, investigated by wgs, mlst and pfge. | in denmark, the incidence of vancomycin-resistant enterococcus faecium (vrefm) has increased since 2012. the aim of this study was to investigate the epidemiology and clonal relatedness of vrefm isolates in danish hospitals in 2012-13 using wgs. the second aim was to evaluate if wgs-based typing could replace pfge for typing of vrefm. | 2015 | 26031466 |
| increased frequency of linezolid resistance among clinical enterococcus faecium isolates from german hospital patients. | linezolid is an antibiotic of last resort for the treatment of infections with vancomycin-resistant enterococci (vre). here we report the increasing prevalence of linezolid resistance among clinical enterococcus faecium strains from german hospital patients. linezolid minimum inhibitory concentrations (mics) were determined for 4461 clinical e. faecium strains isolated between 2008 and 2014. isolates originated from the network of diagnostic laboratories collaborating with the national reference ... | 2015 | 27873661 |
| direct-fed microbials containing lactate-producing bacteria influence ruminal fermentation but not lactate utilization in steers fed a high-concentrate diet. | direct-fed microbials (dfm) have been shown to improve gain and growth efficiency and also modulate ruminal fermentation. in exp. 1,72 beef steers were used to compare a lactate-producing bacterial (lab) dfm consisting primarily of lactobacillus acidophilus and enterococcus faecium,and a lactate-producing and lactate-utilizing (lab/lu) dfm consisting primarily of l. acidophilus and propionibacterium both fed at 10(9) cfu/d. steers were fed a corn-based finishing diet for 153 d and then slaughter ... | 2015 | 26020329 |
| isolation and mode of action of bacteriocin bacc1 produced by nonpathogenic enterococcus faecium c1. | lactic acid bacteria are present in fermented food products and help to improve shelf life and enhance the flavor of the food. they also produce metabolites such as bacteriocins to prevent the growth of undesirable or pathogenic bacteria. in this study, enterococcus faecium c1 isolated from fermented cow milk was able to produce bacteriocin bacc1 and inhibit the growth of selected food-spoilage bacteria. the bacteriocin was purified through 4 steps: ammonium sulfate precipitation, hydrophobic in ... | 2015 | 26004828 |
| prevalence, antimicrobial resistance and genetic lineages of enterococcus spp. from vegetable food, soil and irrigation water in farm environments in tunisia. | the objective of this study was to determine the species, clonal diversity, antibiotic resistance and virulence of enterococci in different environments. seventy-one samples of farm origin (34 of food vegetables, 27 of soil and ten of irrigation water) and 19 samples of vegetables from five markets, were inoculated in slanetz-bartley agar plates supplemented or not with gentamicin (sb-gen and sb plates, respectively) for enterococci recovery. | 2016 | 25988398 |
| molecular characterization of vancomycin-resistant enterococcus faecium strains isolated from carriage and clinical samples in a tertiary hospital, turkey. | this study aimed to determine the presence of vancomycin resistance (vana and vanb) and virulence genes (esp, asa1, gele, ace, hyl, cyla, cpd and ebpa) in vancomycin-resistant enterococcus faecium (vrefm) strains and to analyse the clonal relationships among the strains. e. faecium strains were identified from rectal and clinical specimens by biochemical tests and the api-20 strep kit. susceptibility testing was performed using disc-diffusion and broth-dilution methods. pfge was used for molecul ... | 2015 | 25976005 |
| antimicrobial activity and safety evaluation of enterococcus faecium kq 2.6 isolated from peacock feces. | the objective of this paper was to study antimicrobial activity and safety of enterococcus faecium kq 2.6 (e. faecium kq 2.6) isolated from peacock feces. | 2015 | 25962418 |
| evaluating pediococcus acidilactici and enterococcus faecium nrrl b-2354 as thermal surrogate microorganisms for salmonella for in-plant validation studies of low-moisture pet food products. | pediococcus acidilactici atcc 8042 and enterococcus faecium nrrl b-2354 were investigated as potential surrogates for salmonella serovars using thermal death time kinetics in products such as dry pet foods. the d-values of p. acidilactici atcc 8042, e. faecium nrrl b-2354, and a cocktail of seven salmonella serovars associated with low-moisture products were determined in a preservative-free dry pet food product at moisture levels of 9.1, 17.9, and 27.0% and heated between 76.7 and 87.8°c. the d ... | 2015 | 25951387 |
| magnitude of enterococcal bacteremia in trauma patients admitted for intensive trauma care: a tertiary care experience from south asian country. | bloodstream infection (bsi) and bacteremias due to enterococcus spp. are increasing worldwide with the current need to understand its causes among hospitalized trauma patients. hence, the study was conducted. | 2017 | 25949058 |
| interaction of tgf-β4 and il-17 with iga secretion in the intestine of chickens fed with e. faecium al41 and challenged with s. enteritidis. | the relative mrna expression of iga, tgf-β4, il-17, and concentration of secretory iga (siga) in small intestine of chickens pretreated with enterococcus faecium al41 and challenged with salmonella enteritidis pt4 were studied. salmonella-free day-old chicks (40) cobb 500 breed, were divided into four groups of 10 chicks each (n = 10): control (c), treated with e. faecium al41 strain (efal41), challenged with salmonella enteritidis pt4 (se), and combined (efal41+se). expression of iga and siga c ... | 2015 | 25935756 |
| clinical features of enterococcal bacteremia due to ampicillin-susceptible and ampicillin-resistant enterococci: an eight-year retrospective comparison study. | enterococcus consists human bowel flora, but sometimes behave as an important nosocomial pathogen. in order to identify clinical characteristics that help discriminate between ampicillin-susceptible and ampicillin-resistant enterococcal bacteremia in advance for antimicrobial susceptibility testing, a retrospective eight-year study was carried out in patients with enterococcal bacteremia experienced in saga university hospital, japan. a total of 143 patients were included in the analysis: 85 (59 ... | 2015 | 25935477 |
| synthesis and evaluation of 1,2,4-triazolo[1,5-a]pyrimidines as antibacterial agents against enterococcus faecium. | rapid emergence of antibiotic resistance is one of the most challenging global public health concerns. in particular, vancomycin-resistant enterococcus faecium infections have been increasing in frequency, representing 25% of enterococci infections in intensive care units. a novel class of 1,2,4-triazolo[1,5-a]pyrimidines active against e. faecium is reported herein. we used a three-component biginelli-like heterocyclization reaction for the synthesis of a series of these derivatives based on re ... | 2015 | 25923368 |
| the human gut resistome. | in recent decades, the emergence and spread of antibiotic resistance among bacterial pathogens has become a major threat to public health. bacteria can acquire antibiotic resistance genes by the mobilization and transfer of resistance genes from a donor strain. the human gut contains a densely populated microbial ecosystem, termed the gut microbiota, which offers ample opportunities for the horizontal transfer of genetic material, including antibiotic resistance genes. recent technological advan ... | 2015 | 25918444 |
| enterococcus faecium lke12 cell-free extract accelerates host plant growth via gibberellin and indole-3-acetic acid secretion. | the use of microbial extracts containing plant hormones is a promising technique to improve crop growth. little is known about the effect of bacterial cell-free extracts on plant growth promotion. this study, based on phytohormonal analyses, aimed at exploring the potential mechanisms by which enterococcus faecium lke12 enhances plant growth in oriental melon. a bacterial strain, lke12, was isolated from soil, and further identified as e. faecium by 16s rdna sequencing and phylogenetic analysis. ... | 2015 | 25907061 |
| emergence of daptomycin-resistant enterococcus faecium in a critically ill patient with postoperative intra-abdominal sepsis. | | 2015 | 25904519 |
| epidemiology of vancomycin-resistant enterococci in canadian hospitals (canward study, 2007 to 2013). | of 1,927 enterococcus species isolates collected across canada from 2007 to 2013, 80 (4.2%) were identified as vancomycin-resistant enterococci (vre). vre infections during this time tripled in canadian hospitals, from 1.8% to 6.0% (p = 0.03). all vre were enterococcus faecium, with 90% possessing vana. the prevalence of vanb decreased from 37.5% in 2007 to 0% in 2013 (p < 0.05). the vre were multidrug resistant, but 70.6%, 86.3%, and 100% were susceptible to doxycycline, linezolid, and daptomyc ... | 2015 | 25896693 |
| effects of the probiotic enterococcus faecium and pathogenic escherichia coli strains in a pig and human epithelial intestinal cell model. | the aim of this study has been to elucidate the effect of the probiotic enterococcus faecium ncimb 10415 on epithelial integrity in intestinal epithelial cells and whether pre- and coincubation with this strain can reproducibly prevent damage induced by enterotoxigenic (etec) and enteropathogenic escherichia coli (epec). porcine (ipec-j2) and human (caco-2) intestinal epithelial cells were incubated with bacterial strains and epithelial integrity was assessed by measuring transepithelial electri ... | 2015 | 25883829 |
| persistent strongyloidiasis complicated by recurrent meningitis in an htlv seropositive peruvian migrant resettled in italy. | we describe a case of persistent strongyloidiasis complicated by recurrent meningitis, in a human t cell lymphotropic virus type 1 (htlv-1) seropositive peruvian migrant adult resettled in italy. he was admitted with signs and symptoms of acute bacterial meningitis, reporting four other meningitis episodes in the past 6 years, with an etiological diagnosis of escherichia coli and enterococcus faecium in two cases. he had been previously treated with several antihelmintic regimens not including i ... | 2015 | 25846292 |
| [changes in the phase variant spectra in the populations of lactic acid bacteria under antibiotic treatment]. | effect of the antibiotics kanamycin and ampicillin on growth and phase variation of the populations of four strains of lactic acid bacteria (lactobacillus sp. m67at, l. casei mb, enterococcus faecium m, and e. faecium m3185) was studied. the presence of antibiotics in the medium resulted in a dose-dependent decrease in viable cell numbers and in partial or complete substitution of the dominant s variant with the minor sm and sb variants. the variants differed in colony morphology, as well as in ... | 2015 | 25844438 |
| chloride-inducible expression vector for delivery of antimicrobial peptides targeting antibiotic-resistant enterococcus faecium. | antibiotic-resistant enterococcal infections are a major concern in hospitals where patients with compromised immunity are readily infected. enterococcus faecium bacteria are of particular interest as these pathogens account for over 80% of vancomycin-resistant enterococcal infections. antimicrobial peptides (amps) produced at the site of infection by engineered bacteria may offer a potential alternative to traditional antibiotics for the treatment of resistant bacteria such as e. faecium. for t ... | 2015 | 25841002 |
| non-occlusive mesenteric ischemia after splenic metastasectomy for small-cell lung cancer. | a 68-year-old man presented with severe abdominal pain. seven months earlier, he had received systemic chemotherapy for small-cell lung cancer with solitary metastasis to the spleen, followed by splenectomy. abdominal computed tomography and abdominal arterial angiography showed diffuse ischemia of the mesenteric artery without apparent occlusion. the patient also suffered from septicemia caused by enterococcus faecium. therefore, a diagnosis of non-occlusive mesenteric ischemia (nomi) induced b ... | 2015 | 25832935 |
| modulation of innate immune response, mucosal parameters and disease resistance in rainbow trout (oncorhynchus mykiss) upon synbiotic feeding. | the present study investigates the effects of dietary supplements of galactooligosaccharides (gos), pediococcus acidilactici and p. acidilactici + gos on innate immune response, skin mucus as well as disease resistance of rainbow trout (oncorhynchus mykiss) fingerlings (15.04 ± 0.52 g). after 8 weeks of feeding, several innate immune (lysozyme, alternative complement and respiratory burst activities) and skin mucus parameters (bactericidal activity against streptococcus faecium, streptococcus in ... | 2015 | 25827628 |
| decrease of vancomycin resistance in enterococcus faecium isolates from bloodstream infections in italy from 2003 to 2013. | | 2015 | 25824233 |
| probiotic treatment decreases the number of cd14-expressing cells in porcine milk which correlates with several intestinal immune parameters in the piglets. | modulating the mucosal immune system of neonates by probiotic treatment of their mothers is a promising approach which can only be investigated through the use of animal models. here, we used sows and their piglets to investigate the impact of a bacterial treatment on the sow's milk and on the neonate piglet intestinal immune system. in previous experiments, feed supplementation of sows with the probiotic enterococcus faecium ncimb 10415 during pregnancy and lactation had been shown to affect in ... | 2015 | 25806034 |
| l-lactic acid fermentation by enterococcus faecium: a new isolate from bovine rumen. | to obtain a novel adaptable stain that can produce enantioselective l-lactic acid efficiently, reduce the operational cost of fermentation process and be suitable for scale production. | 2015 | 25801672 |
| effects of ex-vivo and in-vivo treatment with probiotics on the inflammasome in dogs with chronic enteropathy. | inflammasomes coordinate the maturation of il-1β and il-18 in response to danger signals. they are vital for maintenance of intestinal homeostasis and have been linked to chronic intestinal inflammation in humans. probiotics have been advocated as treatment in intestinal inflammation. so far, no study has investigated the role of the inflammasome in canine chronic enteropathy (ce). in this study the intestinal expression of inflammasome components was assessed in ce dogs compared to controls, wh ... | 2015 | 25799280 |
| the stability, and efficacy against penicillin-resistant enterococcus faecium, of the plectasin peptide efficiently produced by escherichia coli. | plectasin, the first defensin extracted from a fungus (the saprophytic ascomycete pseudoplectania nigrella), is attractive as a prospective antimicrobial agent. the purpose of this study was to establish a bacterium-based production system and evaluate the antimicrobial activity of the resulting plectasin. a gene encoding plectasin, with the codon preference of escherichia coli, was optimized based on its amino acid sequence, synthesized using gene-splicing with overlap extension pcr, and insert ... | 2015 | 25791854 |
| a prospective, randomized, blinded, placebo-controlled pilot study on the effect of enterococcus faecium on clinical activity and intestinal gene expression in canine food-responsive chronic enteropathy. | canine chronic enteropathies (ce) are believed to be caused by an aberrant immune response towards the intestinal microbiome. administration of probiotics can alleviate colitis in people. in vitro effects of the probiotic enterococcus faecium ncimb 10415 e1707 (ef) previously have been evaluated using canine cells (e.g., whole blood, intestinal biopsies), but data on in vivo efficacy are lacking. | 2017 | 25776251 |
| vancomycin-resistant enterococcus faecium harbouring vann in canada: a case and complete sequence of pefm12493 harbouring the vann operon. | | 2015 | 25754999 |
| effects of in-feed copper and tylosin supplementations on copper and antimicrobial resistance in faecal enterococci of feedlot cattle. | the objective was to investigate whether in-feed supplementation of copper, at elevated level, co-selects for macrolide resistance in faecal enterococci. | 2015 | 25739516 |
| use of bacteriocin-producing, probiotic strain enterococcus faecium al41 to control intestinal microbiota in farm ostriches. | probiotic enterococci can produce bacteriocins. enterococcus faecium al41 is an enterocin m-producing, probiotic strain which has previously shown beneficial effect in broiler chickens. in this study, it was used to control intestinal microbiota in farm ostriches in a 42-day experiment with an experimental group (eg, 40 ostriches) and a control group (cg, 46). in addition to feed mixture, the ostriches in eg received ent. faecium al41 (10(9) cfu ml(-1); by rifampicin-marked variant) 400 μl per a ... | 2015 | 25732357 |
| a hospital sewage st17 enterococcus faecium with a transferable inc18-like plasmid carrying genes coding for resistance to antibiotics and quaternary ammonium compounds (qacz). | | 2015 | 27873652 |
| persistence of vancomycin resistance in multiple clones of enterococcus faecium isolated from danish broilers 15 years after the ban of avoparcin. | the occurrence and diversity of vancomycin-resistant enterococcus faecium (vref) were investigated in 100 danish broiler flocks 15 years after the avoparcin ban. vref occurred in 47 flocks at low fecal concentrations detectable only by selective enrichment. vancomycin resistance was prevalently associated with a transferable nontypeable plasmid lineage occurring in multiple e. faecium clones. coselection of sequence type 842 by tetracycline use only partly explained the persistence of vancomycin ... | 2015 | 25712360 |
| n-terminus three residues deletion mutant of human beta-defensin 3 with remarkably enhanced salt-resistance. | in this study, we designed and synthesized three n-terminal deletion analogs of human beta-defensin 3 (hbd-3), namely, hbd-3δ4, hbd-3δ7, and hbd-3δ10, to determine the effect of n-terminal residues on the antibacterial activity and salt resistance of these peptides. the antibacterial activities and salt resistance of hbd-3 and its analogs were tested against a broad range of standard and clinically isolated strains. the deletion of nine n-terminal residues significantly reduced the antibacterial ... | 2015 | 25706284 |
| isolation and functional characterization of novel biosurfactant produced by enterococcus faecium. | the objective of the present study was to isolate the biosurfactant (bs) producing lactic acid bacteria (lab) from traditional fermented food (buttermilk) and its functional and structural characterization. bs isolated from strain mrtl9 reduced surface tension from 72.0 to 40.2 mn m(-1). the critical micelle concentration (cmc) of bs was 2.25 mg ml(-1) with emulsification efficiency (e24) after 24 h of 64% against kerosene oil. the cell bound bs was partially purified by silica gel column chroma ... | 2015 | 25674491 |
| in vitro protective effect of lactic acid bacteria on listeria monocytogenes adhesion and invasion of caco-2 cells. | the adhesion of listeria monocytogenes to intestinal endothelial cells is a crucial step in the infection process, which is not well understood. in this study, we evaluated the potential ability of bacteriocin-producing enterococcus faecium, leuconostoc mesenteroides and lactobacillus sakei strains to prevent the adhesion and invasion of eukaryotic cells by ten different l. monocytogenes isolates. the results showed that e. faecium 130 co-cultured with l. monocytogenes was the most effective in ... | 2015 | 25672897 |
| evaluation of enterococcus spp. from rainbow trout (oncorhynchus mykiss, walbaum), feed, and rearing environment against fish pathogens. | the use of lactic acid bacteria of aquatic origin as probiotics constitutes an alternative strategy to the antibiotic treatment for disease control in aquaculture. enterococci are currently used as probiotics in human and animal health. in this study, we evaluated the safety of 64 enterococci isolated from rainbow trout (oncorhynchus mykiss, walbaum), feed and rearing environment, and their antimicrobial activity against 9 fish pathogens. the 64 enterococcal isolates were identified to the speci ... | 2015 | 25671551 |
| enterococcus faecium qu 50: a novel thermophilic lactic acid bacterium for high-yield l-lactic acid production from xylose. | production of optically pure lactic acid from lignocellulosic material for commercial purposes is hampered by several difficulties, including heterofermentation of pentose sugars and high energy consumption by mesophilic lactic acid bacteria. here, we report a novel lactic acid bacterium, strain qu 50, that has the potential to produce optically pure l-lactic acid (≥99.2%) in a homofermentative manner from xylose under thermophilic conditions. strain qu 50 was isolated from egyptian fertile soil ... | 2015 | 25670701 |
| intraepithelial lymphocyte numbers and histomorphological parameters in the porcine gut after enterococcus faecium ncimb 10415 feeding in a salmonella typhimurium challenge. | salmonellae are among the most widespread sources of foodborne infections and salmonella typhimurium, in particular, is correlated with human disease caused by the consumption of contaminated pork. intraepithelial lymphocytes (iel) have early contact with intestinal antigens and play an important role in the detection of pathogenic bacteria. the objective of this study was to determine whether a presumed probiotic enterococcus faecium strain could improve histomorphological and immune system-rel ... | 2015 | 25669594 |
| development of novel antibacterial drugs to combat multiple resistant organisms. | infections due to multidrug-resistant (mdr) bacteria are increasing both in hospitals and in the community and are characterized by high mortality rates. new molecules are in development to face the need of active compounds toward resistant gram-positive and gram-negative pathogens. in particular, the infectious diseases society of america (idsa) has supported the initiative to develop ten new antibacterials within 2020. principal targets are the so-called eskape pathogens (enterococcus faecium, ... | 2015 | 25667169 |
| investigation of vancomycin resistant enterococcus faecium outbreak in neonatal intensive care unit. | enterococci are one of the major agents of community-acquired and nosocomial infections. in this study we aimed to analyze the clonal relation of the vancomycin-resistant enterococci outbreak seen at the neonate intensive care unit (nicu) of uludag university hospital. vancomycin resistance gene was investigated in the enterococcus faecium strains and pulsed field gel electrophoresis (pfge) was used to investigate the genetic relation between outbreak strains. enterococci grown in all patient sa ... | 2014 | 25664041 |
| microbiological analysis of bile and its impact in critically ill patients with secondary sclerosing cholangitis. | secondary sclerosing cholangitis in critically ill patients (ssc-cip) is an emerging disease entity with unfavourable outcome. our aim was to analyze the microbial spectrum in bile of patients with ssc-cip and to evaluate the potential impact on the empiric antibiotic treatment in these patients. | 2015 | 25659761 |
| update on linezolid in vitro activity through the zyvox annual appraisal of potency and spectrum program, 2013. | linezolid showed mic50s and mic90s of 1 μg/ml (for both) against staphylococcus aureus. two s. aureus strains exhibited higher mics (4 to 8 μg/ml) caused by cfr and/or target site mutations, including the first detection of cfr in poland. linezolid (mic50 and mic90, 0.5 and 1 μg/ml) had potent mics against coagulase-negative staphylococci (cons). four cons had mics of 16 to 128 μg/ml due to alterations in 23s rrna and/or l3/l4. linezolid inhibited all enterococci and streptococci at ≤2 μg/ml, ex ... | 2015 | 25645839 |
| microbiological characterization of aquatic microbiomes targeting taxonomical marker genes and antibiotic resistance genes of opportunistic bacteria. | the dissemination of medically relevant antibiotic resistance genes (args) (blavim-1, vana, ampc, ermb, and meca) and opportunistic bacteria (enterococcus faecium/faecalis, pseudomonas aeruginosa, enterobacteriaceae, staphylococcus aureus, and cns) was determined in different anthropogenically influenced aquatic habitats in a selected region of germany. over a period of two years, four differently sized wastewater treatment plants (wwtps) with and without clinical influence, three surface waters ... | 2015 | 25634736 |
| australian enterococcal sepsis outcome programme annual report, 2013. | from 1 january to 31 december 2013, 26 institutions around australia participated in the australian enterococcal sepsis outcome programme (aesop). the aim of aesop 2013 was to determine the proportion of enterococcal bacteraemia isolates in australia that are antimicrobial resistant, and to characterise the molecular epidemiology of the enterococcus faecium isolates. of the 826 unique episodes of bacteraemia investigated, 94.6% were caused by either e. faecalis (56.1%) or e. faecium (38.5%). amp ... | 2014 | 25631594 |
| first complete genome sequence of a probiotic enterococcus faecium strain t-110 and its comparative genome analysis with pathogenic and non-pathogenic enterococcus faecium genomes. | | 2015 | 25619602 |
| screening, identification and characterization of bacteriocins produced by wine-isolated lab strains. | to screen and identify wine-isolated lab strains for bacteriocin production, and to identify and characterize bacteriocins. | 2015 | 25597328 |
| pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha, a traditional maize-based fermented beverage from northwestern argentina. | the diversity of lactic acid bacteria (lab) associated with chicha, a traditional maize-based fermented alcoholic beverage from northwestern argentina, was analyzed using culture-dependent and culture-independent approaches. samples corresponding to 10 production steps were obtained from two local producers at maimará (chicha m) and tumbaya (chicha t). whereas by culture-dependent approach a few number of species (lactobacillus plantarum and weissella viridescens in chicha m, and enterococcus fa ... | 2015 | 25584777 |
| neonatal septicaemia caused by vancomycin resistant enterococcus faecium-a case report. | neonatal bacterial sepsis is one of the major causes of morbidity and mortality in neonates. 10% cases of neonatal bacteraemia and septicaemia are caused by enterococci. the increasing incidence of vancomycin resistant enterococci (vre) is of particular concern because of limited treatment options and increased mortality. we report here a case of neonatal sepsis in a premature baby caused by vancomycin resistant enterococcus faecium van a phenotype from a tertiary care hospital in south india. a ... | 2014 | 25584223 |
| misidentification of streptococcus uberis as a human pathogen: a case report and literature review. | streptococcus uberis is an environmental bacterium responsible for bovine mastitis. it is occasionally described as a human pathogen, though in most cases the identification was based on biochemical phenotyping techniques. this report shows that the biochemical phenotyping may incorrectly identify enterococcus faecium as s. uberis. | 2015 | 25578263 |
| antibacterial activity of enterococcus faecium derived from koopeh cheese against listeria monocytogenes in probiotic ultra-filtrated cheese. | viability of probiotic bacteria in food during maintenance and time of consuming in food has become a challenge in food hygiene and technology and is important for representing their beneficial health effects. the aim of this study was to determine the survival of probiotic enterococcus faecium derived from koopeh cheese added to industrial iranian ultra-filtrated (uf) cheese and screening for antimicrobial activity of enterococcus faecium against listeria monocytogenes during two months of chee ... | 2014 | 25568714 |
| vancomycin-resistance phenotypes, vancomycin-resistance genes, and resistance to antibiotics of enterococci isolated from food of animal origin. | in the present study, 500 raw beef, pork, and chicken meat samples and 100 pooled egg samples were analyzed for the presence of vancomycin-resistant enterococci, vancomycin-resistance phenotypes, and resistance genes. of 141 isolates of enterococci, 88 strains of enterococcus faecium and 53 strains of e. faecalis were identified. the most prevalent species was e. faecium. resistance to ampicillin (n = 93, 66%), ciprofloxacin (n = 74, 52.5%), erythromycin (n = 73, 51.8%), penicillin (n = 59, 41.8 ... | 2015 | 25562594 |
| detection of the classical g2576u mutation in linezolid resistant staphylococcus aureus along with isolation of linezolid resistant enterococcus faecium from a patient on short-term linezolid therapy: first report from india. | linezolid is an effective drug against methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococci (vre). we describe the emergence of linezolid resistance in mrsa and vre from india. | 2015 | 25559997 |
| crosstalk between the microbiome and cancer cells by quorum sensing peptides. | to date, the precise role of the human microbiome in health and disease states remains largely undefined. complex and selective crosstalk systems between the microbiome and mammalian cells are also not yet reported. research up till now mainly focused on bacterial synthesis of virulence factors, reactive oxygen/nitrogen species (ros/rns) and hydrogen sulphide, as well as on the activation of exogenous mutagen precursors by intestinal bacteria. we discovered that certain quorum sensing peptides, ... | 2015 | 25559405 |
| national surveillance on vancomycin-resistant enterococcus faecium in taiwan: emergence and widespread of st414 and a tn1546-like element with simultaneous insertion of is1251-like and is1678. | cases of bacteremia caused by vancomycin-resistant e. faecium (vre-fm) increased significantly in taiwan. the present multicenter surveillance study was performed to reveal the associated epidemiological characteristics. in 2012, 134 non-repetitive vre-fm isolates were prospectively collected from 12 hospitals in taiwan. antimicrobial susceptibility, pulsed-field gel electrophoresis (pfge), multilocus sequence typing (mlst), and analysis of van genes and tn1546 structures were investigated. two ... | 2014 | 25549328 |
| virulence markers of vancomycin resistant enterococci isolated from infected and colonized patients. | the aim of study was to find out the potential pathogenic role of virulence factors elaborated by strains of vancomycin resistant enterococci (vre) isolated from clinical samples and vre colonizing the gastrointestinal tract of hospitalized patients. | 2014 | 25538454 |
| proteome changes underpin improved meat quality and yield of chickens (gallus gallus) fed the probiotic enterococcus faecium. | supplementation of broiler chicken diets with probiotics may improve carcass characteristics and meat quality. however, the underlying molecular mechanism remains unclear. in the present study, 2d-dige-based proteomics was employed to investigate the proteome changes associated with improved carcass traits and meat quality of arbor acres broilers (gallus gallus) fed the probiotic enterococcus faecium. | 2014 | 25532559 |
| phenotypic and genotypic characterization of lactic acid bacteria from traditional cheese in khorramabad city of iran with probiotic potential. | lactic acid bacteria (lab) with proteolitic activity are used as aromatic and antibacterial substances, cholesterol reduces, bile salt hydrolyses, and probiotic. the aims of this project were to isolate and identify natural lab flora involved in traditional fermentation in cheeses of khoramabad city and also to survey their probiotic potential. in order to achieve this goal, lab were isolated and characterized using phenotypic and genotypic methods (pcr-sequencing); in the next stage, they were ... | 2015 | 25519007 |
| linezolid bladder irrigation as adjunctive treatment for a vancomycin-resistant enterococcus faecium catheter-associated urinary tract infection. | to describe the first reported successful use of adjunctive linezolid bladder irrigation. | 2015 | 25515867 |
| pharmacodynamic studies of nitrofurantoin against common uropathogens. | to determine the pharmacokinetic/pharmacodynamic index that best correlates to nitrofurantoin's antibacterial effect, we studied nitrofurantoin activity against common causative pathogens in uncomplicated urinary tract infection (uti). | 2015 | 25515669 |
| in vitro aflatoxin b1 binding capacity by two enterococcus faecium strains isolated from healthy dog faeces. | this study evaluated the binding capacity of aflatoxin b1 (afb1 ) by two enterococcus faecium strains (mf4 and gj40) isolated from faeces from healthy dogs. | 2015 | 25495538 |
| potentially probiotic and bioprotective lactic acid bacteria starter cultures antagonise the listeria monocytogenes adhesion to ht29 colonocyte-like cells. | the capability of five lactic acid bacteria (lab) to counteract the adhesion of listeria monocytogenes to the epithelial intestinal cell line ht29 was studied. the highest adhesion ability to ht29 was achieved by the intestinal strain lactobacillus rhamnosus ctc1679, followed by the meat-derived strains lactobacillus sakei ctc494 and enterococcus faecium ctc8005. surprisingly, the meat strains showed significantly better adhesion to ht29 than two faecal isolates of lactobacillus casei and even s ... | 2015 | 25488261 |
| effect of microbial inoculants on the quality and aerobic stability of bermudagrass round-bale haylage. | the objective of this study was to compare the efficacy of using 4 commercially available microbial inoculants to improve the fermentation and aerobic stability of bermudagrass haylage. we hypothesized that the microbial inoculants would increase the fermentation and aerobic stability of the haylages. bermudagrass (4-wk regrowth) was harvested and treated with (1) deionized water (control); (2) buchneri 500 (b500; lallemand animal nutrition, milwaukee, wi) containing 1×10(5) of pediococcus pento ... | 2015 | 25465545 |
| efficacy of the direct-fed microbial enterococcus faecium alone or in combination with saccharomyces cerevisiae or lactococcus lactis during induced subacute ruminal acidosis. | this study aimed at investigating enterococcus faecium alone or e. faecium in combination with saccharomyces cerevisiae or lactococcus lactis during a subacute ruminal acidosis (sara) challenge. four ruminally fistulated holstein dairy cows were assigned to the following treatments in a 4×4 latin square design: (1) control (con); (2) e. faecium (ef); (3) ef + s. cerevisiae (efsc); (4) ef + l. lactis dsm 11037 (efll). each experimental period consisted of 18 d of adaptation to the respective dire ... | 2015 | 25465534 |
| synthesis and structure-activity relationship of amidine derivatives of 3,4-ethylenedioxythiophene as novel antibacterial agents. | current antibacterial chemotherapeutics are facing an alarming increase in bacterial resistance pressuring the search for novel agents that would expand the available therapeutic arsenal against resistant bacterial pathogens. in line with these efforts, a series of 9 amidine derivatives of 3,4-ethylenedioxythiophene were synthesized and, together with 18 previously synthesized analogs, evaluated for their relative dna binding affinity, in vitro antibacterial activities and preliminary in vitro s ... | 2015 | 25461312 |
| concentration-response studies and modelling of the pharmacodynamics of linezolid: staphylococcus aureus versus enterococcus faecium. | for assessing antibiotic effects, the minimum inhibitory concentration (mic) neglects that most antibiotics display different rates of bacterial killing. time-kill curves, on the other hand, provide details on these killing rates but their interpretation is more complex and hardly standardised. the aim of the present study was to develop an analysis method to easily compare the pharmacodynamics of linezolid (lzd) against staphylococcus aureus and enterococcus faecium via in vitro time-kill curve ... | 2015 | 25455852 |
| effect of vancomycin on the proteome of the multiresistant enterococcus faecium su18 strain. | enterococci are not highly pathogenic bacteria, but the incidence of vancomycin resistance among clinical isolates of this microbial group is steadily increasing, posing a threat to public health. vancomycin-resistant enterococci are currently some of the most recalcitrant hospital-associated pathogens against which new therapies are urgently needed. to understand the molecular mechanisms of bacterial resistance to glycopeptides, we obtained proteomic profiles of the vancomycin-resistant enteroc ... | 2015 | 25449832 |
| the importance of growth kinetic analysis in determining bacterial susceptibility against antibiotics and silver nanoparticles. | routine antibiotics susceptibility testing still relies on standardized cultivation-based analyses, including measurement of inhibition zones in conventional agar diffusion tests and endpoint turbidity-based measurements. here, we demonstrate that common off-line monitoring and endpoint determination after 18-24 h could be insufficient for reliable growth-dependent evaluation of antibiotic susceptibility. different minimal inhibitory concentrations were obtained in 20- and 48 h microdilution pla ... | 2014 | 25426104 |
| isolation and characterization of large spectrum and multiple bacteriocin-producing enterococcus faecium strain from raw bovine milk. | to assess the antimicrobial properties of lactic acid bacteria from tunisian raw bovine milk. | 2015 | 25413921 |
| level of decontamination after washing textiles at 60°c or 70°c followed by tumble drying. | several major outbreaks in healthcare facilities have occurred with the emergence of multi-resistant bacteria. a possible route for dissemination is the hospital textiles and inadequate laundering of them. the aim of this study was to develop an easy-to-use method for simulating the laundering process of hospital textiles, and thereafter apply the method when evaluating the decontaminating efficacy of two different washing temperatures. | 2014 | 25413829 |
| vancomycin revisited - 60 years later. | vancomycin is one of the older antibiotics that has been now in clinical use close to 60 years. earlier on, vancomycin was associated with many side effects including vestibular and renal, most likely due to impurities contained in early vancomycin lots. over the years, the impurities have been removed and the compound has now far less vestibular adverse effects, but still possesses renal toxicity if administered at higher doses rendering trough serum levels of >15 mcg/ml or if administered for ... | 2014 | 25401098 |
| three-year study of health care-associated infections in a turkish pediatric ward. | health care-associated infections (hcais) can cause an increase in morbidity, mortality and costs, especially in developing countries. as information on the epidemiology of hcais in pediatric patientsinturkey is limited, we decided to study the annual incidence and antibiotic resistance patterns in our pediatric ward at marmara university hospital. | 2014 | 25390054 |
| evaluation of the prevalence of insertion element is16 in vancomycin-resistant enterococci strains of enterococcus faecium isolated from transplantology patients from a warsaw hospital between 2010 and 2012. | the study included 79 vancomycin-resistant enterococcus faecium (vre) strains isolated from 33 patients after kidney (n = 19) or liver (n = 14) transplantation; these patients were hospitalized between 2010 and 2012. the strains were obtained from infected persons as well as from carriers. all examined strains were tested for the susceptibility to antimicrobials active against enterococci and for the presence of vana and vanb genes as well as the presence of the insertion sequence is16 considere ... | 2014 | 25380871 |
| use of probiotics to reduce faecal shedding of shiga toxin-producing escherichia coli in sheep. | shiga toxin-producing escherichia coli (stec) are zoonotic, foodborne pathogens of humans. ruminants, including sheep, are the primary reservoirs of stec and there is a need to develop intervention strategies to reduce the entry of stec into the food chain. the initiation of the majority of bacterial, enteric infections involves colonisation of the gut mucosal surface by the pathogen. however, probiotic bacteria can serve to decrease the severity of infection via a number of mechanisms including ... | 2015 | 25380795 |
| effects of synbiotics injected in ovo on regulation of immune-related gene expression in adult chickens. | to determine immunomodulatory effects of synbiotics administered in ovo on immune-related gene expression in adult chickens. | 2014 | 25350090 |
| mass transfer characterization of gamma-aminobutyric acid production by enterococcus faecium cfr 3003: encapsulation improves its survival under simulated gastro-intestinal conditions. | gamma-aminobutyric acid (gaba) production by free and ca-alginate encapsulated cells of enterococcus faecium cfr 3003 was investigated. mass transfer rates characterizing the gaba production process using encapsulated cells were investigated. experiments were performed to investigate external film and internal pore diffusion mass transfer rates. the damkohler and thiele analysis provides a good description of external film and internal pore diffusion resistances, respectively. the experiments re ... | 2015 | 25344450 |
| liofilchem(®) chromatic vre and vancomycin mic test strip detected glycopeptide resistance in a vanb neonatal enterococcus faecium isolate showing alternate vancomycin susceptibility and resistance with biomérieux vitek2. | a 1-month old neonate urine sample yielded vanb enterococcus faecium; nevertheless, the isolate alternatively showed susceptibility and resistance to vancomycin with biomérieux vitek2 (cards ast592, ast632, ast586), while glycopeptide resistance was detected by liofilchem(®) vancomycin mic test strip and disc along with the chromatic vre chromogenic medium. this communication emphasizes that, as vanb gene may be heterogeneously expressed within a given enterococcus population, glycopeptide resis ... | 2014 | 25337280 |
| membrane active phenylalanine conjugated lipophilic norspermidine derivatives with selective antibacterial activity. | natural and synthetic membrane active antibacterial agents offer hope as potential solutions to the problem of bacterial resistance as the membrane-active nature imparts low propensity for the development of resistance. in this report norspermidine based antibacterial molecules were developed that displayed excellent antibacterial activity against various wild-type bacteria (gram-positive and gram-negative) and drug-resistant bacteria (methicillin-resistant staphylococcus aureus, vancomycin-resi ... | 2014 | 25335118 |
| characterization of a multidrug resistance plasmid from enterococcus faecium that harbours a mobilized bcrabdr locus. | | 2015 | 25324422 |
| the first report of the vanc₁ gene in enterococcus faecium isolated from a human clinical specimen. | the vanc₁ gene, which is chromosomally located, confers resistance to vancomycin and serves as a species marker for enterococcus gallinarum. enterococcus faecium tj4031 was isolated from a blood culture and harbours the vanc₁gene. polymerase chain reaction (pcr) assays were performed to detect vanxyc and vantc genes. only the vanxyc gene was found in the e. faecium tj4031 isolate. the minimum inhibitory concentrations of vancomycin and teicoplanin were 2 µg/ml and 1 µg/ml, respectively. real-tim ... | 2014 | 25317698 |
| (18)f-fdg pet/ct detected a septic focus corresponding to a small periurethral abscess in a patient with bacteremia due to enterococcus faecium. | | 2017 | 25304846 |
| antimicrobial activity of a quinuclidine-based ftsz inhibitor and its synergistic potential with β-lactam antibiotics. | filamenting temperature-sensitive mutant z (ftsz) is an essential cell division protein that cooperates in the formation of the cytokinetic z-ring in most bacteria and has thus been recognized as a promising antimicrobial drug target. we have recently used a structure-based virtual screening approach to identify pyrimidine-linked quinuclidines as a novel class of ftsz inhibitors. in this study, we further investigated the antibacterial properties of one of the most potent compounds (quinuclidine ... | 2015 | 25293977 |
| molecular epidemiology of vancomycin-resistant enterococci isolated from non-tertiary-care and tertiary-care hospitals in korea. | this study compared the molecular characteristics of vancomycin-resistant enterococcus faecium (vref) isolates recovered from 20 non-tertiary-care hospitals (36 isolates) and three tertiary-care hospitals (26 isolates) in diverse geographical areas of korea from october 2010 to april 2011. all isolates carried the vana gene only, but 42% and 73% of non-tertiary and tertiary-care isolates expressed the vanb phenotype (teicoplanin minimum inhibitory concentration ⩽16 μg/ml). all isolates harboured ... | 2014 | 25267406 |
| urinary tract infections during the first year after renal transplantation: one center's experience and a review of the literature. | the aim of the study was to evaluate the incidence, clinical manifestations, microbiology, risk factors, and the influence of utis on renal graft function. | 2014 | 25251447 |
| decolonization of gastrointestinal carriage of vancomycin-resistant enterococcus faecium: case series and review of literature. | prolonged asymptomatic carriage of vancomycin-resistant enterococci (vre) in the gastrointestinal tract and the lack of effective decolonization regimen perpetuate the endemicity of vre in the healthcare settings. | 2014 | 25248287 |
| aetiology and antibiotic resistance issues regarding urological procedures. | there are specific indications in urological procedures [transurethral resection of the prostate (turp), transurethral resection of the bladder (turb), endoscopic procedures, and all interventions classified as contaminated or dirty] requiring antibiotic prophylaxis. most postoperative infections are caused by enterococci of the gram-positive strains and enterobacteriaceae of the gram-negative ones. as reported by the european center for disease prevention and control (ecdc), there are increasin ... | 2014 | 25245707 |
| potential for reduction of streptogramin a resistance revealed by structural analysis of acetyltransferase vata. | combinations of group a and b streptogramins (i.e., dalfopristin and quinupristin) are "last-resort" antibiotics for the treatment of infections caused by gram-positive pathogens, including methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus faecium. resistance to streptogramins has arisen via multiple mechanisms, including the deactivation of the group a component by the large family of virginiamycin o-acetyltransferase (vat) enzymes. despite the structural elucida ... | 2014 | 25223995 |
| use of a direct-fed microbial product as a supplement during the transition period in dairy cattle. | two studies were conducted. the objective of the first study was to assess the effects of a direct-fed microbial (dfm) product on dry matter intake, milk yield, milk components, disease incidence, and blood metabolites in dairy cattle. the objective of the second study was to assess the effects of dfm on apparent total-tract nutrient digestibility (attd). one hundred twenty primiparous and multiparous holstein cows housed in a tiestall facility at the university of guelph were used in study 1, a ... | 2014 | 25218748 |
| effects of oral enterococcus faecium strain dsm 10663 ncimb 10415 on diarrhoea patterns and performance of sucking piglets. | effects of probiotic enterococcus faecium dms 10663 ncimb 10415 on diarrhoea and performance of sucking piglets were evaluated in a double-blind, placebo-controlled study. piglets from treatment group (tg, n=56) and placebo group (pg, n=53) sows were included in the study. immediately after birth and at day 2 and 3 post natum, each of the tg piglets received 2.8×10(9) colony forming units (cfu) per os. from day 4 until weaning (day 26), 1.26×10(9) cfu e. faecium/piglet were given twice a day via ... | 2015 | 25213026 |
| emergence of vanb phenotype-vana genotype enterococcus faecium clinical isolate in bulgaria. | | 2014 | 25203657 |
| survival of salmonella tennessee, salmonella typhimurium dt104, and enterococcus faecium in peanut paste formulations at two different levels of water activity and fat. | long-term survival of heat-stressed salmonella tennessee, salmonella typhimurium dt104, and enterococcus faecium was evaluated in four model peanut paste formulations with a combination of two water activity (aw) levels (0.3 and 0.6) and two fat levels (47 and 56%) over 12 months at 20 ± 1°c. prior to storage, the inoculated peanut paste formulations were heat treated at 75°c for up to 50 min to obtain an approximately 1.0-log reduction of each organism. the cell population of each organism in e ... | 2014 | 25198585 |
| enterococcus faecium pbp5-s/r, the missing link between pbp5-s and pbp5-r. | during a study to investigate the evolution of ampicillin resistance in enterococcus faecium, we observed that a number of e. faecium strains, mainly from the recently described subclade a2, showed pbp5 sequences in between pbp5-s and pbp5-r. these hybrid pbp5-s/r patterns reveal a progression of amino acid changes from the s form to the r form of this protein; however, these changes do not strictly correlate with changes in ampicillin mics. | 2014 | 25182648 |
| comparison of two methods for purification of enterocin b, a bacteriocin produced by enterococcus faecium w3. | this study aimed to compare two different approaches for the purification of enterocin b from enterococcus faecium strain w3 based on the observation that the bacteriocin was found both in cell associated form and in culture supernatant. the first approach employed ammonium sulfate precipitation, cation-exchange chromatography, and sequential reverse-phase high-performance liquid chromatography. the latter approach exploited a ph-mediated cell adsorption-desorption method to extract cell-bound b ... | 2015 | 25181442 |
| biotechnical paving of recombinant enterocin a as the candidate of anti-listeria agent. | enterocin a is a classic iia bacteriocin isolated firstly from enterococcus faecium ctc492 with selective antimicrobial activity against listeria strains. however, the application of enterocin a as an anti-listeria agent has been limited due to its very low native yield. the present work describes high production of enterocin a through codon optimization strategy and its character study. | 2014 | 25163588 |
| a multicentre hospital outbreak in sweden caused by introduction of a vanb2 transposon into a stably maintained prum-plasmid in an enterococcus faecium st192 clone. | the clonal dissemination of vanb-type vancomycin-resistant enterococcus faecium (vrefm) strains in three swedish hospitals between 2007 and 2011 prompted further analysis to reveal the possible origin and molecular characteristics of the outbreak strain. a representative subset of vrefm isolates (n = 18) and vancomycin-susceptible e. faecium (vsefm, n = 2) reflecting the spread in time and location was approached by an array of methods including: selective whole genome sequencing (wgs; n = 3), m ... | 2014 | 25153894 |
| enterococcal endocarditis complicated with ruptured infected-intracranial aneurysm: with pharmacokinetic-pharmacodynamic documentation in proof of the successful antimicrobial treatment. | a 74-year-old man presented with sudden onset of aphasia and apraxia. magnetic resonance image (mri) of the brain disclosed a left frontal hemorrhage. the concomitant low grade fever suggestive of infection was unresponsive to cefazolin 1 g q12h, and refractory to piperacillin (pipc) 2 g q8h. blood culture grew enterococci, establishing together with echocardiography the diagnosis of infective endocarditis. the angiography revealed cerebral hemorrhage to have resulted from the rupture of the inf ... | 2014 | 25153621 |