| evaluating periodontal risk for patients at risk of or suffering from atherosclerosis: recent biological hypotheses and therapeutic consequences. | cardiovascular disease, such as atherosclerosis, is the main cause of mortality in developed countries. most atherosclerosis risk factors have been identified and are treated, improving patient cardiovascular status and reducing mortality, but some remain unknown. periodontal disease is generally defined as inflammatory disease initiated by accumulation of dental bacterial plaque, leading to the destruction of tissues that support the teeth. severe forms have a high prevalence (15% of the popula ... | 2011 | 21693372 |
| porphyromonas gingivalis enzymes enhance infection with human metapneumovirus in vitro. | relatively recently discovered, the human metapneumovirus (hmpv) is a human pathogen with worldwide prevalence, accounting for a substantial percentage of respiratory tract diseases. concurrent viral and bacterial infections enable intricate mechanisms of cooperation between pathogens, which complicate the symptoms and outcome of the disease. such bilateral interactions are based on the modulation of bacterial growth on epithelium pathologically altered during viral illness and the modulation of ... | 2011 | 21715599 |
| antimicrobial sesquiterpenoids from laurus nobilis l. | activity-guided fractionations of leaf extracts from laurus nobilis l. led to the isolation of a known sesquiterpene lactone, deacetyl laurenobiolide (1). compound 1 showed antimicrobial activity against periopathic pathogens (actinomyces viscosus, porphyromonas gingivalis, prevotella intermedia and actinobacillus actinomycetemcomitans), opportunistic gram-positive bacteria (staphylococcus aureus and streptococcus pyogenes) and fungi (candida albicans, cryptococcus neoformans and aspergillus fum ... | 2011 | 21678158 |
| target region selection is a critical determinant of community fingerprints generated by 16s pyrosequencing. | pyrosequencing of 16s rrna genes allows for in-depth characterization of complex microbial communities. although it is known that primer selection can influence the profile of a community generated by sequencing, the extent and severity of this bias on deep-sequencing methodologies is not well elucidated. we tested the hypothesis that the hypervariable region targeted for sequencing and primer degeneracy play important roles in influencing the composition of 16s pyrotag communities. subgingival ... | 2011 | 21738596 |
| identification of porphyromonas gingivalis lipopolysaccharide-binding proteins in human saliva. | porphyromonas gingivalis causes periodontal diseases and its lipopolysaccharide (lps) is considered as a major virulence factor responsible for pathogenesis. since initial recognition of p. gingivalis lps (pg.lps) in the oral cavity might be crucial for the host response, we identified pg.lps-binding proteins (pg.lps-bps) using pg.lps-immobilized beads and a high-resolution mass spectrometry. lps purified from p. gingivalis was conjugated onto n-hydroxysuccinimidyl-sepharose(-«) 4 fast flow bead ... | 2011 | 21742380 |
| complete genome sequence of the bacterium porphyromonas gingivalis tdc60, which causes periodontal disease. | porphyromonas gingivalis is a black-pigmented asaccharolytic anaerobe and a major causative agent of periodontitis. here, we report the complete genome sequence of p. gingivalis strain tdc60, which was recently isolated from a severe periodontal lesion in a japanese patient. | 2011 | 21705612 |
| isolation of the new anacardic acid 6-[16'z-nonadecenyl]-salicylic acid and evaluation of its antimicrobial activity against streptococcus mutans and porphyromonas gingivalis. | a new anacardic acid, 6-[16'z-nonadecenyl]-salicylic acid (1), along with seven known compounds, 6-[8'z-pentadecenyl] salicylic acid (15:1 anacardic acid) (2), 6-nonadecenyl salicylic acid (anacardic acid 19:0) (3), 6-pentadecyl salicylic acid (anacardic acid 15:0) (4), masticadienonic acid (5), 3+¦-hydroxymasticadienonic acid (6), 3-epi-oleanolic acid (7) and +¦-sitosterol, were isolated from the bark of amphipterygium adstringens using a bioassay-guided fractionation method. the structure of t ... | 2011 | 21815722 |
| [gums and aorta; tobacco and the brain]. | | 2011 | 21721210 |
| chlorhexidine varnishes effectively inhibit porphyromonas gingivalis and streptococcus mutans - an in vivo study. | chlorhexidine varnish (cervitec- ivoclar vivadent- liechtenstein) is a sustained-release delivery system that can provide protection against white spots and gingivitis, which are common iatrogenic side effects of orthodontic treatment. chlorhexidine in varnish form does not depend on patient compliance, does not stain teeth or alter taste sensation like the mouth rinse. | 2010 | 21760672 |
| Use of host- and bacteria-derived salivary markers in detection of periodontitis: a cumulative approach. | In the present study, we propose a novel diagnostic approach, using 3 different salivary markers, representing periodontal pathogen burden, inflammation, and tissue degradation, for detecting periodontitis. The salivary concentrations of Porphyromonas gingivalis, interleukin-1+¦, and matrix metalloproteinase-8, available from salivary specimens of 165 subjects (84 subjects with advanced periodontitis and 81 controls), were calculated together to obtain a cumulative risk score (CRS). In the calcu ... | 2011 | 21725158 |
| impact of porphyromonas gingivalis inoculation on ligature-induced alveolar bone loss. a pilot study in rats. | periodontitis is a polymicrobial infection characterized by the loss of connective tissue attachment, periodontal ligament and alveolar bone. the aim of this study was to evaluate the impact of porphyromonas gingivalis inoculation on the ligature-induced alveolar bone loss (abl) model in rats. | 2011 | 21726226 |
| Simultaneous detection of periodontal pathogens in subgingival plaque and placenta of women with hypertension in pregnancy. | BACKGROUND: There are many studies documenting increased prevalence of periodontal infection in women with preeclampsia. But, very few studies have attempted to establish causal relationship between the two. OBJECTIVE: To find out causal circumstantial evidence by isolating specific periodontal pathogens in oral and placental samples. MATERIALS AND METHODS: Antenatal periodontal screening and subgingival plaque collection was carried out in ten women with hypertension in pregnancy and ten normot ... | 2011 | 21830010 |
| Schisandra chinensis +¦-iso-cubebenol induces heme oxygenase-1 expression through PI3K/Akt and Nrf2 signaling and has anti-inflammatory activity in Porphyromonas gingivalis lipopolysaccharide-stimulated macrophages. | Heme oxygenase-1 (HO-1) is a potent anti-inflammatory molecule that regulates pro-inflammatory mediators. Several studies have indicated that HO-1 expression is induced by a variety of stimuli such as lipopolysaccharide (LPS), cytokines, oxidative stress, and antioxidant phytochemicals. In this study, we assessed the anti-inflammatory effects of a novel +¦-iso-cubebenol isolated from dried fruits of Schisandra chinensis in human macrophage THP-1 cells and investigated the involvement of HO-1 sig ... | 2011 | 21840424 |
| filifactor alocis has virulence attributes that can enhance its persistence under oxidative stress conditions and mediate invasion of epithelial cells by porphyromonas gingivalis. | filifactor alocis, a gram-positive anaerobic rod, is one of the most abundant bacteria identified in the periodontal pockets of periodontitis patients. there is a gap in our understanding of its pathogenicity and ability to interact with other periodontal pathogens. to evaluate the virulence potential of f. alocis and its ability to interact with porphyromonas gingivalis w83, several clinical isolates of f. alocis were characterized. f. alocis showed nongingipain protease and sialidase activitie ... | 2011 | 21825062 |
| porphyromonas gingivalis induces myocarditis and/or myocardial infarction in mice and il-17a is involved in pathogenesis of these diseases. | although an association between periodontitis and cardiovascular diseases has been suggested, the role of porphyromonas gingivalis in cardiovascular diseases is not clear. in this study, we examined whether experimental bacteremia of p. gingivalis causes cardiovascular diseases and investigated the mechanism of pathogenesis of cardiovascular diseases induced by p. gingivalis. | 2011 | 21683342 |
| polymicrobial periodontal pathogen transcriptomes in calvarial bone and soft tissue. | porphyromonas gingivalis, treponema denticola, and tannerella forsythia are consistently associated with adult periodontitis. this study sought to document the host transcriptome to a p. gingivalis, t. denticola, and t.forsythia challenge as a polymicrobial infection using a murine calvarial model of acute inflammation and bone resorption. mice were infected with p. gingivalis, t. denticola, and t. forsythia over the calvaria, after which the soft tissues and calvarial bones were excised. a muri ... | 2011 | 21896157 |
| oral infection with porphyromonas gingivalis and systemic cytokine profile in c57bl/6.kor-apoe(shl) mice. | miyauchi s, maekawa t, aoki y, miyazawa h, tabeta k, nakajima t, yamazaki k. oral infection with porphyromonas gingivalis and systemic cytokine profile in c57bl/6.kor- apoe(shl) mice. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01441.x.©2011 john wiley & sons a/s background and objective: periodontal infection affects atherosclerotic diseases, such as coronary heart diseases. mouse models have revealed that oral infection with porphyromonas gingivalis induces changes in inflammatory- a ... | 2011 | 22097957 |
| IL-23-producing CD68(+) macrophage-like cells predominate within an IL-17-polarized infiltrate in chronic periodontitis lesions. | To analyse antigen-presenting cells (APCs), such as dendritic cells (DCs), macrophages (Mo) or B cells depending on the regional site of chronic periodontitis (CP), and to investigate their relation to Th17 cells. | 2011 | 21883359 |
| Dental Abnormalities in Eight Captive Giant Pandas (Ailuropodamelanoleuca) in China. | Dental data from eight adult giant pandas (Ailuropoda melanoleuca) (four females and four males) were collected at the Beijing Zoo from February 2009 to July 2010. Examination findings were recorded in dental charts. All the pandas had different degrees of tooth wear. Incisors, canines and second premolars had the most abnormalities. Five animals had caries; molars were the most affected. Chip fractures were found in seven teeth (incisor, canine and premolar) of five pandas; two had complicated ... | 2011 | 21906751 |
| immune evasion strategies of porphyromonas gingivalis. | porphyromonas gingivalis is strongly correlated with chronic periodontitis. its chronic persistence in the periodontium depends on its ability to evade host immunity without inhibiting the overall inflammatory response, which is actually beneficial for this and other periodontal bacteria. indeed, the inflammatory exudate (gingival crevicular fluid) is a source of essential nutrients, such as peptides and hemin-derived iron. in this review, i discuss how p. gingivalis can promote its adaptive fit ... | 2011 | 22162663 |
| a five-species transcriptome array for oral mixed-biofilm studies. | oral polymicrobial interactions and biofilm formation are associated with initiation and progression of caries, gingivitis, and periodontitis. transcriptome studies of such interactions, allowing a first mechanistic insight, are hampered by current single-species array designs. | 2011 | 22194794 |
| Solution NMR structures reveal a distinct architecture and provide first structures for protein domain family PF04536. | The protein family (Pfam) PF04536 is a broadly conserved domain family of unknown function (DUF477), with more than 1,350 members in prokaryotic and eukaryotic proteins. High-quality NMR structures of the N-terminal domain comprising residues 41-180 of the 684-residue protein CG2496 from Corynebacterium glutamicum and the N-terminal domain comprising residues 35-182 of the 435-residue protein PG0361 from Porphyromonas gingivalis both exhibit an a/ß fold comprised of a four-stranded ß-sheet, thre ... | 2011 | 22198206 |
| Highly Specific Protease-Based Approach for Detection of Porphyromonas gingivalis in Diagnosis of Periodontitis. | Porphyromonas gingivalis is associated with the development of periodontitis. Here we describe the development of a highly specific protease-based diagnostic method for the detection of P. gingivalis in gingival crevicular fluid. Screening of a proteolytic peptide substrate library, including fluorogenic dipeptides that contain d-amino acids, led to the discovery of five P. gingivalis-specific substrates. Due to the presence of lysine and arginine residues in these substrates, it was hypothesize ... | 2012 | 22075590 |
| Aerosolized clindamycin is superior to aerosolized dexamethasone or clindamycin-dexamethasone combination in the treatment of severe Porphyromonas gingivalis aspiration pneumonia in an experimental murine model. | ABSTRACT Adjunctive corticosteroid treatment to reduce excessive local inflammatory response in pneumonia is controversial. To study the effects of an early local adjunct dexamethasone treatment on the course of pneumonia and inflammatory/cytokine response, mice were intratracheally inoculated with live Porphyromonas gingivalis and treated with either clindamycin (C), dexamethasone (D), C+D combination, or were not treated (Pg). Six mice from each group were euthanized at 6, 24, 72, and 168 ho ... | 2011 | 22149928 |
| Microbiological analysis of gingivitis in pediatric patients under orthodontic treatment. | The aim of this study was to determine the relationship between gingival inflammation and changes in bacteria of the gingival sulcus in children in orthodontic treatment with brackets. | 2011 | 22185242 |
| antibiofilm effects of azithromycin and erythromycin on porphyromonas gingivalis. | antibiotic resistance of biofilm-grown bacteria contributes to chronic infections, such as marginal and periapical periodontitis, which are strongly associated with porphyromonas gingivalis. concurrent azithromycin (azm) administration and mechanical debridement improve the clinical parameters of periodontal tissue in situ. we examined the in vitro efficacy of azm against p. gingivalis biofilms. the susceptibilities of adherent p. gingivalis strains 381, hw24d1, 6/26, and w83 to azm, erythromyci ... | 2011 | 21911560 |
| [enteric gram negative rods and unfermented of glucose bacteria in patients with peri-implant disease]. | implants can be colonized by microorganisms from oral biofilms and may affect peri-implant tissues health. among these bacteria, pathogens typically associated with periodontitis can be found, such as aggregatibacter actinomycetemcomitans and porphyromonas gingivalis, as well as gram negative enteric bacilli not typically associated with periodontal diseases. | 2011 | 22159480 |
| chronic periodontitis with multiple risk factor syndrome: a case report. | multiple risk factor syndrome is a clustering of cardiovascular risk factors, such as diabetes, dyslipidemia, hypertension, and obesity associated epidemiologically with insulin resistance. this report describes the clinical course of a patient suffering from severe periodontitis with multiple risk factor syndrome, and discusses the association between periodontal infection and systemic health. | 2011 | 21913601 |
| Antimicrobial evaluation of diterpenes from Copaifera langsdorffii oleoresin against periodontal anaerobic bacteria. | The antimicrobial activity of four labdane-type diterpenes isolated from the oleoresin of Copaifera langsdorffii as well as of two commercially available diterpenes (sclareol and manool) was investigated against a representative panel of microorganisms responsible for periodontitis. Among all the evaluated compounds, (-)-copalic acid (CA) was the most active, displaying a very promising MIC value (3.1 µg mL-1; 10.2 µM) against the key pathogen (Porphyromonas gingivalis) involved in this infectio ... | 2011 | 22101836 |
| Role of p38 MAPK Pathway in Porphyromonas gingivalis LPS-Induced VCAM-1 Expression in Human Aortic Endothelial Cells. | Background: Porphyromonas gingivalis lipopolysaccharide (LPS) has been reported to induce the expression of vascular cell adhesion molecule-1 (VCAM-1) in vascular endothelial cells. This finding suggests potential roles for P. gingivalis in the pathogenesis of atherosclerosis. However, the mechanism involved in P. gingivalis LPS-induced VCAM-1 production in endothelial cells remains unclear. Methods: Quantitative real-time polymerase chain reaction and Western blotting were used respectively to ... | 2011 | 22122523 |
| Quantitative analysis of microbiota in saliva, supragingival, and subgingival plaque of Chinese adults with chronic periodontitis. | OBJECTIVE: The aim of this study was to determine the profiles of periodontopathogenic bacteria in a Chinese population using quantitative real-time polymerase chain reaction (qRT-PCR). MATERIALS AND METHODS: Twenty-four periodontally healthy Chinese subjects and 60 patients with chronic periodontitis (CP) were enrolled in this cross-sectional study. qRT-PCR was used to quantify Aggregatibacter actinomycetemcomit ... | 2011 | 22169888 |
| Isoflavonoids and Coumarins from Glycyrrhiza uralensis: Antibacterial Activity against Oral Pathogens and Conversion of Isoflavans into Isoflavan-Quinones during Purification. | Phytochemical investigation of a supercritical fluid extract of Glycyrrhiza uralensis has led to the isolation of 20 known isoflavonoids and coumarins, and glycycarpan (7), a new pterocarpan. The presence of two isoflavan-quinones, licoriquinone A (8) and licoriquinone B (9), in a fraction subjected to gel filtration on Sephadex LH-20 is due to suspected metal-catalyzed oxidative degradation of licoricidin (1) and licorisoflavan A (2). The major compounds in the extract, as well as 8, were evalu ... | 2011 | 22074222 |
| relationship between serum antibody titres to porphyromonas gingivalis and hs-crp levels as inflammatory markers of periodontitis. | objective: the present study was designed to investigate whether titres of antibody to two strains of porphyromonas gingivalis, fdc381 and su63, are associated with serum high-sensitivity c-reactive protein (hs-crp) levels in japanese periodontitis patients. design: forty-nine patients with moderate to advanced periodontitis and 40 periodontally healthy control subjects were included in this study. hs-crp levels and antibody titres to p. gingivalis were measured at baseline and reassessment 3-4 ... | 2011 | 22172404 |
| Low-abundance biofilm species orchestrates inflammatory periodontal disease through the commensal microbiota and complement. | Porphyromonas gingivalis is a low-abundance oral anaerobic bacterium implicated in periodontitis, a polymicrobial inflammatory disease, and the associated systemic conditions. However, the mechanism by which P. gingivalis contributes to inflammation and disease has remained elusive. Here we show that P. gingivalis, at very low colonization levels, triggers changes to the amount and composition of the oral commensal microbiota leading to inflammatory periodontal bone loss. The commensal microbiot ... | 2011 | 22036469 |
| bacterial-killing effect of atmospheric pressure non-equilibrium plasma jet and oral mucosa response. | recently, plasma sterilization has attracted increasing attention in dental community for the atmospheric pressure non-equilibrium plasma jet (apnps), which is driven by a kilohertz pulsed dc power, may be applied to the dental and oral diseases. however, it is still in doubt whether apnps can effectively kill pathogenic bacteria in the oral cavity and produce no harmful effects on normal oral tissues, especially on normal mucosa. the aim of this study was to evaluate the bacterial-killing effec ... | 2011 | 22173512 |
| pathogenic microbes and community service through manipulation of innate immunity. | the periodontal pathogen porphyromonas gingivalis undermines major components of innate immunity, such as complement, toll-like receptors (tlr), and their crosstalk pathways. at least in principle, these subversive activities could promote the adaptive fitness of the entire periodontal biofilm community. in this regard, the virulence factors responsible for complement and tlr exploitation (gingipain enzymes, atypical lipopolysaccharide molecules, and fimbriae) are released as components of readi ... | 2012 | 21948363 |
| effect of oil pulling on halitosis and microorganisms causing halitosis: a randomized controlled pilot trial. | oil pulling therapy has been used extensively as a traditional indian folk remedy for many years for strengthening teeth, gums, and jaws and to prevent decay, oral malodor, bleeding gums and dryness of throat, and cracked lips. | 2011 | 21911944 |
| Bactericidal properties of pradofloxacin against veterinary pathogens. | Pradofloxacin is a new veterinary 8-cyano-fluoroquinolone developed for use against bacterial infections in dogs and cats involving both aerobic and anaerobic bacteria. The minimal bactericidal concentrations have been determined against clinical isolates of Staphylococcus pseudintermedius, Staphylococcus aureus, Escherichia coli, Pasteurella multocida, Streptococcus canis, Proteus spp., Fusobacterium spp., Porphyromonas gingivalis and Prevotella species. A subset of these species was selected, ... | 2011 | 22209121 |
| Positive correlations between presence of gram negative enteric rods and Porphyromonas gingivalis in subgingival plaque. | The association between Gram negative enteric rods and Porphyromonas gingivalis in periodontal diseases has received little attention in the literature. Thus, the aim of this study was to investigate the associations between Gram negative enteric rods, Porphyromonas gingivalis and clinical parameters of periodontal disease. The prevalence of Gram-negative enteric rods and P. gingivalis were examined in patients with chronic periodontitis. Chi-square and Mann-Whitney tests were used to determine ... | 2011 | 22010401 |
| synergistic effects of antibiotics and an n-acyl homoserine lactone analog on porphyromonas gingivalis biofilms. | aims: to investigate the effects of the combined application of an n-acyl homoserine lactone (hsl) analog and antibiotics on biofilms of porphyromonas gingivalis, a major pathogen of periodontal disease. methods and results: antibiotics used were cefuroxime, ofloxacin and minocycline. a flow-cell model was used for biofilm formation. samples were divided into four groups: control, analog-treated, antibiotic-treated and combined application groups. biofilm cell survival was determined using ade ... | 2011 | 22093286 |
| The periodontal pathogen Porphyromonas gingivalis induces the Epstein-Barr virus lytic switch transactivator ZEBRA by histone modification. | Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that usually results in latent infection of B cells. The EBV BZLF1 gene product ZEBRA is a master regulator of the transition from latency to the lytic replication cycle. In the latent state, hypoacetylation of histone proteins in the BZLF1 promoter by histone deacetylases (HDACs) is primarily involved in maintaining EBV latency. Although the mechanism that regulates the switch between latency and lytic replication has been a central res ... | 2011 | 22178321 |
| antimicrobial advances in treating periodontal diseases. | antibiotics are generally an efficient means of treating bacterial infections, and therefore are an obvious candidate in the treatment of periodontal diseases. systemically and locally administered antimicrobial agents of all kinds have been evaluated in multiple clinical trials. the vast majority of studies have tested antibiotics as adjuncts to non-surgical debridement. no regime has demonstrated superiority over systemically administered amoxicillin and metronidazole in the treatment of any c ... | 2012 | 22142961 |
| transition metal ions induce carnosinase activity in pepd-homologous protein from porphyromonas gingivalis. | aminoacylhistidine dipeptidase (ec 3.4.13.3; also xaa-his dipeptidase, carnosinase, or pepd) catalyzes the cleavage and release of an n-terminal amino acid, which is usually a neutral or hydrophobic residue, from an xaa-his dipeptide or degraded peptide fragment. pepd enzyme is found extensively in prokaryotes and eukaryotes, and belongs to the metallopeptidase family m20, a part of the metallopeptidase h (mh) clan. carnosine is a naturally occurring dipeptide (β-alanyl-l-histidine) present in m ... | 2012 | 22001095 |
| Immune responses to porphyromonas gingivalis infection suppress systemic inflammatory response in experimental murine model. | Periodontitis is a localized infectious disease caused by periodontopathic bacteria such as Porphyromonas gingivalis (P. gingivalis), and the severity correlates to significance of immune responses. Recently, it has been reported that periodontitis is associated with the development of systemic disease such as diabetes and atherosclerosis because of increasing invasion of oral pathogens to the circulation. However, the association between local and systemic infectious responses is still unclear. ... | 2011 | 21880208 |
| Clinical and Microbial Evaluation of the Effects on Gingivitis of a Mouth Rinse Containing an Enteromorpha linza Extract. | Abstract Enteromorpha linza, a green alga, has been recognized as a potential source of natural antimicrobial and antifungal compounds. We previously reported that an E. linza extract strongly inhibited the growth of Prevotella intermedia and Porphyromonas gingivalis. The principal objective of this study was to evaluate the clinical effect of a mouth rinse containing the E. linza extract on gingivitis disease, as measured by the plaque index (PI), gingival index (GI), and bleeding on probing ... | 2011 | 22145775 |
| Involvement of the TREM-1/DAP12 pathway in the innate immune responses to Porphyromonas gingivalis. | Porphyromonas gingivalis, is a Gram-negative obligate oral anaerobic bacterium highly implicated in periodontal disease, the most prevalent chronic inflammatory disease, but recent evidence also indicates a potential contribution to systemic inflammation. The Triggering Receptor Expressed on Myeloid cells 1 (TREM-1) is a cell surface receptor of the immunoglobulin superfamily, which, along with its adaptor signalling molecule DAP12, is involved in immune response to bacterial and fungal infectio ... | 2011 | 21967868 |
| [Role of several periodontopathogenic microorganisms and tlr4 gene Asp299Gly polymorphism in atherosclerosis pathogenesis]. | Establishment of presence of periodonto-pathogenic microorganisms in atherosclerosis plaque and surrounding tissues, and possible relation of development of atherosclerosis and TLR4 gene Asp299Gly polymorphism in ischemic heart disease patients (IHD). | 2011 | 22145357 |
| Oral biofilm analysis of palatal expanders by fluorescence in-situ hybridization and confocal laser scanning microscopy. | Confocal laser scanning microscopy (CLSM) of natural heterogeneous biofilm is today facilitated by a comprehensive range of staining techniques, one of them being fluorescence in situ hybridization (FISH). We performed a pilot study in which oral biofilm samples collected from fixed orthodontic appliances (palatal expanders) were stained by FISH, the objective being to assess the three-dimensional organization of natural biofilm and plaque accumulation. FISH creates an opportunity to stain cells ... | 2011 | 22041974 |
| identification of interspecies interactions affecting porphyromonas gingivalis virulence phenotypes. | periodontitis is recognized as a complex polymicrobial disease, however, the impact of the bacterial interactions among the 700-1,000 different species of the oral microbiota remains poorly understood. we conducted an in vitro screen for oral bacteria that mitigate selected virulence phenotypes of the important periodontal pathogen, porphyromonas gingivalis. | 2011 | 22022641 |
| evidence for graft colonization with periodontal pathogens in lung transplant recipients. | summary bronchiolitis obliterans syndrome (bos) is a major cause of late graft dysfunction in lung transplant recipients. there is increasing evidence that beside alloimmunologic injury also non-alloimmunologic inflammatory conditions may raise the risk of acute and chronic rejection. the oral cavity represents a possible reservoir for pathogenic bacteria due to its close anatomical proximity. in this pilot study, the presence of pathogenic periodontal bacteria in the oral cavity as well as in t ... | 2011 | 22203528 |
| Free lipid A isolated from P. gingivalis LPS is contaminated with phosphorylated dihydroceramide lipids: Recovery in diseased dental samples. | Recent reports indicate that Porphyromonas gingivalis mediates alveolar bone loss or osteoclast modulation through engagement of Toll like receptor 2 (TLR2) though the factors responsible for TLR2 engagement have yet to be determined. LPS and lipid A, lipoprotein, fimbria and phosphorylated dihydroceramides of P. gingivalis have been reported to activate host cell responses through engagement of TLR2. LPS and lipid A are the most controversial in this regard because conflicting evidence has been ... | 2011 | 22144487 |
| Levels of periodontal pathogens in neonatal gastric aspirates and possible maternal sites of origin. | Maternal periodontal infection has been recognized as a risk factor for preterm and low birthweight infants. It is suspected that pathogens causing periodontal disease may translocate to the amniotic cavity and so contribute to triggering an adverse pregnancy outcome. This study aimed to determine levels and proportions of periodontal bacteria in neonatal gastric aspirates obtained from complicated pregnancies and the respective maternal oral and vaginal samples using a quantitative polymerase c ... | 2011 | 21896155 |
| porphyromonas gingivalis gingipains trigger a proinflammatory response in human monocyte-derived macrophages through the p38a mitogen-activated protein kinase signal transduction pathway. | porphyromonas gingivalis, the major etiologic agent of chronic periodontitis, produces a broad spectrum of virulence factors, including arg- and lys-gingipain cysteine proteinases. in this study, we investigated the capacity of p. gingivalis gingipains to trigger a proinflammatory response in human monocyte-derived macrophages. both arg- and lys-gingipain preparations induced the secretion of tnf-a and il-8 by macrophages. stimulation of macrophages with arg-gingipain a/b preparation at the high ... | 2010 | 22069588 |
| [quorum sensing inhibitor brominated furanone affects porphyromonas gingivalis biofilm formation]. | to study the influence of the quorum sensing inhibitor brominated furanone on porphyromonas gingivalis (p. gingivalis) biofilm formation. | 2011 | 22165111 |
| In situ gastrointestinal protection against anthrax edema toxin by single-chain antibody fragment producing lactobacilli. | ABSTRACT: BACKGROUND: Anthrax is caused by the bacterium Bacillus anthracis and is regarded as one of the most prominent bioterrorism threats. Anthrax toxicity is induced by the tripartite toxin complex, composed of the receptor-binding anthrax protective antigen and the two enzymatic subunits, lethal factor and edema factor. Recombinant lactobacilli have previously been used to deliver antibody fragments directed against surface epitopes of a variety of pathogens, including Streptococcus mutan ... | 2011 | 22185669 |
| comprehensive transcriptome analysis of the periodontopathogenic bacterium porphyromonas gingivalis w83. | high-density tiling microarray and rna sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium porphyromonas gingivalis. the compiled p. gingivalis transcriptome profiles were based on total rna samples isolated from three different laboratory culturing conditions, and the strand-specific transcription profiles generated covered the entire genome, including both protein coding and noncoding regions. the transcription profiles revealed various operon s ... | 2012 | 22037400 |
| Cell entry and exit by periodontal pathogen via recycling pathway. | In the oral cavity, gingival epithelial cell (GEC) layers function as an innate host defense system to prevent intrusion by periodontal bacteria. Nevertheless, Porphyromonas gingivalis, the most well-known periodontal pathogen, can enter GECs and pass through the epithelial barrier into deeper tissues. An intracellular location is considered advantageous for bacteria to escape from immune surveillance by the host as well as antibiotic pressure, leading to intracellular persistence, multiplicatio ... | 2011 | 22046471 |
| pathogens and atherosclerosis: update on the potential contribution of multiple infectious organisms to the pathogenesis of atherosclerosis. | it is currently unclear what causes the chronic inflammation within atherosclerotic plaques. one emerging paradigm suggests that infection with bacteria and/or viruses can contribute to the pathogenesis of atherosclerosis either via direct infection of vascular cells or via the indirect effects of cytokines or acute phase proteins induced by infection at non-vascular sites. this paradigm has been supported by multiple epidemiological studies that have established positive associations between th ... | 2011 | 22012133 |
| lymphangiogenesis is induced during development of periodontal disease. | lymphangiogenesis, the formation of new lymphatics, is associated with chronic inflammation and tissue injury, and its role is to enhance lymphatic flow, immune cell transport, and antigen clearance. it is unknown if lymphangiogenesis takes place during periodontal disease development, and we hypothesized that growth of lymphatic vessels occurs in gingiva during development of periodontitis in mice. inflammation was induced in gingiva with porphyromonas gingivalis gavage, and bone resorption was ... | 2012 | 21979132 |
| abrogation of neuraminidase reduces biofilm formation, capsule biosynthesis, and virulence of porphyromonas gingivalis. | the oral bacterium porphyromonas gingivalis is a key etiological agent of human periodontitis, a prevalent chronic disease that affects up to 80% of the adult population worldwide. p. gingivalis exhibits neuraminidase activity. however, the enzyme responsible for this activity, its biochemical features, and its role in the physiology and virulence of p. gingivalis remain elusive. in this report, we found that p. gingivalis encodes a neuraminidase, pg0352 (sia(pg)). transcriptional analysis showe ... | 2012 | 22025518 |
| presence of porphyromonas gingivalis in gingival squamous cell carcinoma. | periodontal disease has been recently linked to a variety of systemic conditions such as diabetes, cardiovascular disease, preterm delivery, and oral cancer. the most common bacteria associated with periodontal disease, porphyromonas gingivalis (p. gingivalis) has not yet been studied in the malignant gingival tissues. the objective of this study was to investigate the presence of p. gingivalis in specimens from squamous cell carcinoma patients. we have performed immunohistochemical staining to ... | 2011 | 22010579 |
| detection of periodontal pathogens in newborns and children with mixed dentition. | we report the age-related prevalence of red complex periodontal pathogens, porphyromonas gingivalis, treponema denticola, and tannerella forsythia, along with four strains of orange complex pathogens. the bacteria present in samples isolated from tongue, cheek, and subgingival sulcus in edentulous newborns and children with mixed dentition were monitored by polymerase chain reaction (pcr). p. gingivalis was not detected in any site of any subject in the two groups tested. however, t. denticola w ... | 2011 | 21928086 |
| expression and secretion levels of th1 and th2 cytokines in patients with aggressive periodontitis. | the role of th1 and th2 cytokines in the pathogenesis of aggressive periodontitis has not been previously examined. the aim of this study was to analyse the expression and production of il-2, ifn-γ, il-4 and il-13 in cd4+ cells from the peripheral blood of patients with aggressive periodontitis (agp) and periodontally healthy controls. gene expression was analysed in inactivated and activated cd4+ cells by real-time pcr. cells were activated for 4, 8 and 24 h with anti-cd3/cd28 antibody, phytohe ... | 2011 | 22065246 |
| [effects of porphyromonas gingivalis with different fima genotypes on vascular cell adhesion molecule-1 and intercellular adhesion molecule-1 production by human umbilical vein endothelial cells]. | to investigate the effect of porphyromonas gingivalis (pg) with different fima genotypes on vascular cell adhesion molecule-1 (vcam-1) and intercellular adhesion molecule-1 (icam-1) production by human umbilical vein endothelial cells (huvec). | 2011 | 21914375 |
| [Calcium metabolism associated with oral diseases. Cardiovascular diseases and periodontal diseases]. | Accumulated evidence has strongly suggested that the long-term effects of periodontal diseases can be linked to more serious systemic conditions such as cardiovascular diseases, diabetes, and complications of pregnancy. Especially, a prevalence of coronary heart disease was found to be significantly increased in patients with periodontitis after adjusting for risk factors such as smoking, diabetes, alcohol intake, obesity, and blood pressure. Furthermore, various studies have shown that Porphyro ... | 2012 | 22201098 |
| receptor activator of nuclear factor kappa b ligand antagonists inhibit tissue inflammation and bone loss in experimental periodontitis. | the purpose of this study was to assess the role of anti-bone resorptive agents and an anti-inflammatory compound in murine porphyromonas gingivalis (p. gingivalis)-induced periodontitis. | 2011 | 22092474 |
| porphyromonas endodontalis lipopolysaccharides induce rankl by mouse osteoblast in a way different from that of escherichia coli lipopolysaccharide. | porphyromonas endodontalis lipopolysaccharide (lps) has been shown to have a high positive rate in infected root canals and symptomatic apical periodontitis. it may play an integral role as a potent stimulator of inflammatory cytokines involved in apical lesions. the receptor activator of nuclear factor-κb ligand (rankl) has been proven to be the key regulator of bone remodeling. this study investigated p. endodontalis lps-induced rankl production and lps signaling in mouse osteoblasts. | 2011 | 22099899 |
| [clinical effect of chitosan in bacterial vaginosis therapy]. | determination of antibacterial activity of acid-soluble chitosan in bacterial vaginosis therapy in women of reproductive age and comparison of therapy results of traditional scheme and local administration of acid-soluble chitosan. | 2011 | 22145355 |
| analysis of symptomatic and asymptomatic primary root canal infections in adult norwegian patients. | this molecular study analyzed the microbiota of primary root canal infections from adult norwegian patients. | 2011 | 21846535 |
| extracellular deoxyribonuclease production by periodontal bacteria. | palmer lj, chapple ilc, wright hj, roberts a, cooper pr. extracellular deoxyribonuclease production by periodontal bacteria. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01451.x © 2011 john wiley & sons a/s background and objective: whilst certain bacteria have long been known to secrete extracellular deoxyribonuclease (dnase), the purpose in microbial physiology was unclear. recently, however, this enzyme has been demonstrated to confer enhanced virulence, enabling bacteria to evade the ... | 2011 | 22150619 |
| porphyromonas gingivalis antigens differently participate in the proliferation and cell death of human pbmc. | objective: modulation of cell-mediated immunity by microorganisms in periodontal diseases has been widely studied; however, the proliferative activity and/or programmed death of mononuclear cells under periodontopathogenic stimuli are not yet well understood. the aim of this study was to investigate in vitro proliferation and death of peripheral blood mononuclear cells (pbmc) upon stimulation with porphyromonas gingivalis (pg) antigens. design: in 19 patients with chronic periodontitis (cp) and ... | 2011 | 21944906 |
| vima-dependent modulation of acetyl-coa levels and lipid a biosynthesis can alter virulence in porphyromonas gingivalis. | the poryphyromonas gingivalis vima protein has multifunctional properties that can modulate several of its major virulence factors. to further characterize vima, p. gingivalis fll406 carrying additional vima gene and vima-defective mutant in a different p. gingivalis genetic background were evaluated. the vima-defective mutant (fll451) in the p. gingivalis 33277 genetic background showed a similar phenotype to the vima-defective mutant (fll92) in the p. gingivalis w83 genetic background. in cont ... | 2011 | 22144476 |
| inhibitory effect of quercetin on periodontal pathogens. | periodontal disease and associated bone loss can severely hinder orthodontic treatment. actinobacillus actinomycetemcomitans (aa) and porphyromonas gingivalis (pg) are bacteria which are strongly associated with this type of bone loss. quercetin, a unique flavonoid, which is found in many common foods including apples, onions and tea may have a effect against these bacteria. | 2008 | 22073470 |
| efficacy of locally-delivered doxycycline microspheres in chronic localized periodontitis and on porphyromonas gingivalis. | aim: this study aimed to assess the efficacy and effect of locally-delivered doxycycline microspheres with scaling and root planing in periodontal pocket therapy and on porphyromonas gingivalis, respectively. methods: twenty sites with a probing pocket depth of 4-6 mm were divided into two groups: a control group consisting of scaling and root planing, with one application of doxycycline microspheres only at baseline, and a test group consisting of scaling and root planing, with an application ... | 2011 | 22180217 |
| the subgingival microbiota of papillon-lefèvre syndrome. | background: there is little information about the microbiological profiles of periodontal lesions in papillon-lefèvre syndrome (pls) and the significance of bacteria in the pathogenesis of periodontitis in these patients. this comprehensive analysis of the subgingival microbiota in pls patients used 16s rrna clonal analysis and the 16s rrna-based human oral microbe identification microarray (homim). methods: thirteen pls patients, from 7 unrelated families, volunteered for this microbiological s ... | 2011 | 22141356 |
| in vitro antibacterial activity of galls of quercus infectoria olivier against oral pathogens. | the galls of quercus infectoria are commonly used in malay traditional medicine to treat wound infections after childbirth. in india, they are employed traditionally as dental applications such as that in treatment of toothache and gingivitis. the aim of the present study was to evaluate the antibacterial activity of galls of quercus infectoria olivier against oral bacteria which are known to cause dental caries and periodontitis. methanol and acetone extracts were screened against two gram-posi ... | 2012 | 22203875 |
| comparison of the virulence of exopolysaccharide-producing prevotella intermedia to exopolysaccharide non-producing periodontopathic organisms. | evidence in the literature suggests that exopolysaccharides (eps) produced by bacterial cells are essential for the expression of virulence in these organisms. secreted epss form the framework in which microbial biofilms are built. | 2011 | 21864411 |
| antimicrobial effects of o-cymen-5-ol and zinc, alone & in combination in simple solutions and toothpaste formulations. | this study aimed to evaluate antimicrobial effects of an o-cymen-5-ol/zinc system. | 2011 | 21762153 |
| association of the fcγriib-nt645+25a/g polymorphism with the expression level of the fcγriib receptor, the antibody response to porphyromonas gingivalis and the severity of periodontitis. | sugita n, iwanaga r, kobayashi t, yoshie h. association of the fcγriib-nt645+25a/g polymorphism with the expression level of the fcγriib receptor, the antibody response to porphyromonas gingivalis and the severity of periodontitis. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01411.x. © 2011 john wiley & sons a/s background and objective: human fcγriib is an immunoglobulin g (igg) receptor that inhibits the activation of b lymphocytes through cross-linking with the b-cell receptor via im ... | 2011 | 21906057 |
| Increased serum PCSK9 concentrations are associated with periodontal infection but do not correlate with LDL cholesterol concentration. | Periodontal disease increases the risk of atherothrombotic disease, and high concentrations of low density lipoprotein (LDL) cholesterol are considered to be involved; however, the underlying mechanisms are largely unknown. Recent studies demonstrated that proprotein convertase subtilisin/kexin type 9 (PCSK9) plays a critical role in circulating LDL cholesterol concentrations. The aim of the present study is to analyze serum PCSK9 concentrations and their relation to lipoprotein concentrations i ... | 2012 | 22001517 |
| effect of a self-etching adhesive containing an antibacterial monomer on clinical periodontal parameters and subgingival microbiologic composition in orthodontic patients. | the aims of this study were to evaluate the effect of a self-etching adhesive system containing an antibacterial monomer on periodontal health and subgingival microbiologic composition in orthodontic patients and to compare it with a conventional adhesive system. | 2011 | 21967952 |
| gingival changes during pregnancy: iii. impact of clinical, microbiological, immunological and socio-demographic factors on gingival inflammation. | aims: to identify predictor variables involved in exacerbated gingival inflammation associated with pregnancy. material and methods: in this cohort study, 48 pregnant and 28 non-pregnant women without periodontitis were included. the pregnant women were evaluated in the first, second and third trimester and at 3 months postpartum, whilst the non-pregnant women were evaluated twice, with a 6-month interval. at each visit, clinical [plaque index (pli) and gingival index (gi)], hormonal (salivary p ... | 2011 | 22092526 |
| thyrotoxicosis with post-treatment hypothyroidism in a patient with acute suppurative thyroiditis due to porphyromonas. | background: acute suppurative thyroiditis (ast) is a rare, life-threatening thyroid infection characterized by a tender neck mass and fever. as these features are shared with self-limited subacute thyroiditis (sat), it is important to differentiate between the two disorders. patient findings: we report a case of ast in a 21-year-old woman who presented with steadily worsening throat pain for 3 weeks, a tender left neck mass, and thyrotoxicosis. she was initially given prednisone for treatment ... | 2011 | 22136210 |
| salivary levels of antibacterial peptide (ll-37/hcap-18) and cotinine in periodontitis patients. | background: the purpose of this study was to examine the relationship between salivary ll-37 levels and clinical severity in chronic periodontitis (cp) patients. the presence/absence of four periodontopathic bacteria and salivary cotinine levels were also examined in order to assess the impact of these factors on ll-37 production. methods: unstimulated salivary samples were collected from 69 cp patients. salivary concentrations of ll-37 and cotinine were measured by enzyme-linked immunosorbent ... | 2011 | 21942788 |
| evaluation of the subgingival microbiota of alcoholic and non-alcoholic individuals. | the aim of this study was to evaluate the composition of the subgingival microbiota of alcoholic and non-alcoholic individuals. | 2011 | 21864643 |
| [initial study of biological characterization and virulence of porphyromonas gingivalis pg0839-defective mutant]. | to study the effect of pg0839 gene form porphyromonas gingivalis (p. gingivalis) on biological characterization and virulence in a murine model of soft tissue destruction, and further to provide evidence for clarifying the gene function of pg0839. | 2011 | 22109357 |
| differential expression and adherence of porphyromonas gingivalis fima genotypes. | porphyromonas gingivalis is a primary pathogen involved in the initiation and progression of adult chronic periodontitis. its colonization on oral surfaces is a necessary first step leading to infection. fima, a subunit protein of major (long) fimbriae, is a well-known virulence factor. based on its nucleotide sequence, fima is classified into several genotypes. we compared here the transcriptional levels of the fima gene in several p. gingivalis strains using real-time polymerase chain reaction ... | 2011 | 22053966 |
| development and evaluation of new primers for pcr-based identification of type ii fima of porphyromonas gingivalis. | for more accurate pcr-based identification of porphyromonas gingivalis harboring genotype ii fima, the most prevalent type in periodontitis patients, a new primer set was developed and evaluated. the previous type ii primers hybridized to the dna of p. gingivalis strains harboring type ib as well as type ii fima, while the new primers specifically amplified only the dna fragment of type ii fima. in the investigation using mixed bacterial culture and 155 clinical samples from peri-implantitis pat ... | 2011 | 22067036 |
| cathepsin b, d, and l regulation in cyclosporin a-mediated gingival hyperplasia of a patient with sarcoidosis. | cyclosporin a (csa) is an immunosuppressant with side effects including gingival hyperplasia. sarcoidosis is a systemic disease characterized by granulomas. here, we report on a rare case of sarcoidosis with gingival hyperplasia to clarify whether clinical observation corresponds to in vitro results. | 2011 | 21888018 |
| predominant bacterial species in subgingival plaque in dogs. | dahlén g, charalampakis g, abrahamsson i, bengtsson l, falsen e. predominant bacterial species in subgingival plaque in dogs. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01440.x.©2011 john wiley & sons a/s background and objective: the dog has been used extensively for experimental and microbiological studies on periodontitis and peri-implantitis without detailed knowledge about the predominant flora of the subgingival plaque. this study was designed to evaluate the predominant cultivab ... | 2011 | 22181039 |
| Improved periodontal health and cardiovascular risk. | Background: Previous studies have demonstrated variable effects on systemic inflammatory and immune responses following improved periodontal health. This study examined changes in serum levels of the inflammatory mediators IL-1ß, IL-6, TNF-a and sICAM-1, and antibodies to Porphyromonas gingivalis, human heat shock protein (hHSP) 60 and P. gingivalis GroEL following improvement in periodontal health in high cardiovascular (CV) risk and low CV-risk patients. Methods: Patients retrospectively sel ... | 2011 | 22126343 |
| Porphyromonas gingivalis: a clonal pathogen?: Diversities in housekeeping genes and the major fimbriae gene. | The introduction of multilocus sequence typing (MLST) in infectious disease research has allowed standardized typing of bacterial clones. Through multiple markers around the genome, it is possible to determine the sequence type (ST) of bacterial isolates to establish the population structure of a species. For the periodontal pathogen, Porphyromonas gingivalis, the MLST scheme has been established at www.pubmlst.org/pgingivalis, and data from the database indicate a high degree of genetic diversi ... | 2011 | 22125739 |
| Protease inhibitor levels in periodontal health and disease. | Kretschmar S, Yin L, Roberts F, London R, Flemmig TT, Arushanov D, Kaiyala K, Chung WO. Protease inhibitor levels in periodontal health and disease. J Periodont Res 2011; doi: 10.1111/j.1600-0765.2011.01425.x. © 2011 John Wiley & Sons A/S Background and Objective: Our previous study showed that protease inhibitors were attenuated by the periodontal pathogen Porphyromonas gingivalis in cultured gingival epithelial cells. We hypothesize that fewer protease inhibitors would be present in more adva ... | 2011 | 22029638 |
| Antimicrobial resistance determinants among anaerobic bacteria isolated from footrot. | Antibiotic resistance has been evaluated among 36 Gram negative and anaerobic bacilli (10 Bacteroides, 11 Prevotella, 7 Porphyromonas and 8 Fusobacterium strains) isolated from clinical cases of caprine and ovine footrot (necrotic pododermatitis). The initial analysis on this bacterial consortium evaluates the relationships existing among antimicrobial resistance determinants, phenotype expression and mobilization potential. The Bacteroides strains were generally resistant to penicillins, first- ... | 2011 | 22177890 |
| tnf receptor-associated factor 6 suppression inhibits inflammatory response to porphyromonas gingivialis in human periodontal ligament cells. | periodontitis is a group of inflammatory diseases caused by microorganisms. porphyromonas gingivalis, a gram-negative bacteria, is strongly associated with the onset of periodontitis. tumor necrosis factor (tnf) receptor-associated factor 6 (traf6) represents an important target in the regulation of many disease processes, including immunity, inflammation, and osteoporosis. the aim of this study was to investigate the role of traf6 for inflammatory response in p gingivalis-infected human periodo ... | 2011 | 21909504 |
| Trefoil Factors in Saliva and Gingival Tissues of Patients With Chronic Periodontitis. | Background: Trefoil factors (TFFs) are secreted molecules which are involved in cytoprotection against tissue damage, and the immune response. TFFs have been detected in saliva and oral tissues, but their clinical significance has never been investigated in patients with chronic periodontitis. The objective of our study was to determine if TFF expression in saliva and gingival tissues was associated with periodontal pathology. Methods: Saliva and gingival tissue samples were collected from 25 n ... | 2011 | 22181686 |
| antibacterial activity of povidone-iodine against an artificial biofilm of porphyromonas gingivalis and fusobacterium nucleatum. | objective: to investigate the antibacterial activity of povidone-iodine (pvp-i) on an artificial dual species biofilm of periodontal pathogens. design: porphyromonas gingivalis or fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (pcm) tissue culture inserts. after incubation for 72h, pvp-i solutions were applied to the biofilm for the time period ranging from 0.5 to 5min. after addition of a deactivator, each pcm was removed and the biofilm on the pcm was s ... | 2011 | 21958970 |
| Comparative evaluation of two structurally related flavonoids, isoliquiritigenin and liquiritigenin, for their oral infection therapeutic potential. | Isoliquiritigenin (1) and liquiritigenin (2) are structurally related flavonoids found in a variety of plants. The purpose of this study was to perform a comparative analysis of biological properties of these compounds in regard to their therapeutic potential for oral infections. Compound 1 demonstrated significant antibacterial activity against three major periodontopathogens, Porphyromonas gingivalis, Fusobacterium nucleatum, and Prevotella intermedia. In contrast, 2 exerted less pronounced ef ... | 2011 | 21866899 |
| Outer membrane vesicles of Porphyromonas gingivalis elicit a mucosal immune response. | We previously reported that mutation of galE in Porphyromonas gingivalis has pleiotropic effects, including a truncated lipopolysaccharide (LPS) O-antigen and deglycosylation of the outer membrane protein OMP85 homolog. In the present study, further analysis of the galE mutant revealed that it produced little or no outer membrane vesicles (OMVs). Using three mouse antisera raised against whole cells of the P. gingivalis wild type strain, we performed ELISAs to examine the reactivity of these ant ... | 2011 | 22022548 |
| The destructive effects of butyrate on the cell envelope of Helicobacter pylori. | Helicobacter pylori can be detected in the oral cavity, although most studies involve the use of PCR techniques. The growth of H. pylori is influenced by various factors in the mouth such as the oral microflora, saliva and other antimicrobial substances, all of which make colonization of the oral cavity by H. pylori difficult. In this study, we analyzed the effect of the cell supernatant of a representative periodontal bacterium Porphyromonas gingivalis on H. pylori and found that the cell super ... | 2011 | 22194341 |