| establishment of the steady state in glucose-limited chemostat cultures of klebsiella pneumoniae. | to investigate the relationship between growth rate and concentration of the nutrient that limits growth, 'klebsiella aerogenes' nctc 418 (k. pneumoniae) was grown in a glucose-limited chemostat. the actual time required to establish a steady-state glucose concentration exceeded that expected theoretically. apparently, there is a long-term adaptation of the cells to nutrient limitation. as yet, it is not clear whether this has a phenotypic or genetic origin. in the final steady state, the depend ... | 1987 | 3309157 |
| effect of bacteroides fragilis cellular components on chemotactic activity of polymorphonuclear leukocytes towards escherichia coli. | chemotaxis of polymorphonuclear leukocytes (pmnl) in response to cell components of bacteroides fragilis alone or in combination with escherichia coli was evaluated. e. coli produced much more powerful chemotactic factors than b. fragilis. the culture filtrate (cf), outer membrane (om) preparation, and lipopolysaccharide (lps) of b. fragilis slightly stimulated chemotactic activity of pmnl. the culture filtrate and om preparation were capable of inhibiting the chemotaxis of pmnl in response to t ... | 1987 | 3309320 |
| a morphological study of the action of equine anti-lipopolysaccharide plasma on gram-negative bacteria. | three strains of gram-negative bacteria--one each of escherichia coli, klebsiella pneumoniae and enterobacter sp.--were treated with anti-lipopolysaccharide hyperimmune equine plasma (anti-lps) or non-immune control plasma and examined by scanning electronmicroscopy. within a few minutes of treatment with anti-lps, bacteria were agglutinated. evidence of cell membrane destruction was observed shortly thereafter and total cell disintegration and disruption occurred within 1-2 h. in contrast, non- ... | 1987 | 3309324 |
| a set of positively regulated flagellar gene promoters in caulobacter crescentus with sequence homology to the nif gene promoters of klebsiella pneumoniae. | the study reported here describes nuclease s1 mapping of the in-vivo transcription start sites of transcription units i and iii of the hook gene cluster of caulobacter crescentus. we show that transcription units i and ii of this flagellar (fla) gene cluster, which have divergent promoters with transcription start sites separated by 218 nucleotides, are under positive transcriptional control by genes in transcription unit iii. the promoters of transcription units i, ii, and iii were compared wit ... | 1987 | 3309346 |
| [effects of beta-lactamases on the antibacterial activity of carumonam and aztreonam against klebsiella sp. in comparison with 4 other beta-lactams]. | carumonam and aztreonam are two similar monocyclic beta lactam antibiotics. we have studied their antibacterial activities on klebsiella, in comparison with cefotaxime, cefoxitin, cefotetan and imipenem. in the case of k. pneumoniae (53 strains) the antibacterial activities of the two monobactams are very similar. on the contrary, with k. oxytoca (22 strains) carumonam is significantly more active. this situation seems to be justified by an improved stability of carumonam to beta lactamases. in ... | 1987 | 3309817 |
| [epidemiology, clinical aspects and treatment of nosocomial septicemias]. | one hundred one nosocomial septicemias (ns) were studied among 461 cases of septicemias (22%) collected by french septicemia expert system group during 1985. the mean-age of the patients was 58 +/- 23 years while it was 56 +/- 23 years in the 360 community acquired septicemias (cas). the source of septicemia was found more frequently in ns than in cas (79/101 versus 223/360; p less than 0.002): mainly urinary tract and intravenous therapy in ns, urinary tract, gastrointestinal and biliary origin ... | 1987 | 3309823 |
| [septicemia of urinary origin. epidemiology and initial antibiotic treatment]. | the ses group was formed by the "ligue française pour la prévention des maladies infectieuses" to create a diagnostic and therapeutic expert system on septicemia. 461 cases of septicemia were thus collected from january 1 to december 31, 1985. 83 out of these septicemias had an urinary tract portal of entry, 21 were contracted in hospital. the mean age was 65 +/- 21 years (57 +/- 20 years for the 461 cases ns). there were 46 women and 37 men, i.e., a sex-ratio apposite to that of the overall pop ... | 1987 | 3309824 |
| [treatment of post-traumatic and post-neurosurgical bacterial meningitis with ceftriaxone alone or in combination with fosfomycin]. | from 1984 to 1986, 13 patients (10 adults, 3 children) with bacterial meningitis following neurosurgery or traumatism were given ceftriaxone alone 6 times at a dose of 40 mg/kg one iv injection per day, or in association 7 times with fosfomycin at a dose of 200 mg/kg/day, 3 iv perfusions every 4 h. the bacteriological diagnosis was confirmed in 9 cases (3 staphylococcus aureus, 4 streptococcus pneumoniae, 1 klebsiella, 1 peptococcus). in vitro neither synergy nor antagonism were observed between ... | 1987 | 3309825 |
| [use of the ms2 automated system in the determination of the activity of 5 antiseptic drugs: quality control]. | the study of growth curves of klebsiella pneumoniae and staphylococcus aureus in presence of five antiseptics, established using a ms2 abbott system is presented. from our results, the advantages of automation after the adaptation of the method for the determination of bactericidal properties are examined. this technique may be proposed for the quality control of such drugs. | 1987 | 3309836 |
| in vitro and in vivo antibacterial activities of cs-807, a new oral cephalosporin. | cs-807 is a new oral prodrug of r-3746, a cephalosporin derivative, with potent in vitro and in vivo antibacterial activity against both gram-positive and gram-negative bacteria. the susceptibility of about 1,200 clinical isolates to r-3746 was determined by the agar dilution method. ninety percent or more of pathogens such as staphylococcus aureus, streptococci, escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, indole-positive and indole-negative proteus spp., providencia rettgeri, a ... | 1987 | 3310868 |
| effects of bacterial endotoxin on the ciliary activity in the in vitro eustachian tube. | we have used a tissue culture technique and a photoelectric method to examine the direct effect of lipopolysaccharide (lps) on the ciliary activity present in the eustachian tube. since lps possesses the major part of the biological activity of endotoxin, our results show clearly that lps deteriorates the ciliary activity in a dose-response fashion: lps does not deteriorate the ciliary activity up to 168 h if its concentration is 1 ng/ml or less; 10 ng/ml lps can cause deterioration of the cilia ... | 1987 | 3310987 |
| nitrogenase of klebsiella pneumoniae. rhodanese-catalysed restoration of activity of the inactive 2fe species of the fe protein. | the inactive 2fe species of the fe protein of the nitrogenase of klebsiella pneumoniae was generated by treating oxidized fe protein (kp2) with mgatp and chelator. incubation of the 2fe species of kp2 with the sulphurtransferase rhodanese in the presence of thiosulphate, ferric citrate and reduced lipoate reproducibly restored activity. the extent of restoration of activity depended on the molar ratio of 2fe kp2 to rhodanese and was time-dependent. re-activation did not occur in the reaction mix ... | 1987 | 3311031 |
| role of lipopolysaccharide and complement in susceptibility of klebsiella pneumoniae to nonimmune serum. | the role of lipopolysaccharide (lps) in the susceptibility of klebsiella pneumoniae to serum and the mechanism of complement activation by serum-susceptible (sers) strains were investigated. the classical and alternative complement pathways are involved in serum killing of susceptible k. pneumoniae strains. the lps composition seems to play a very important role in the serum bactericidal reaction, while capsular polysaccharide from this bacterium does not play any role. high-molecular-weight lps ... | 1987 | 3312009 |
| the influence of flucloxacillin and amoxicillin with clavulanic acid on the aerobic flora of the alimentary tract. | in a randomized study, 42 patients undergoing extensive maxillo-facial surgery (correction of the position of the mandible or maxilla by using autologous bone transplants) received prophylactically ten-day courses of either flucloxacillin or amoxicillin with clavulanic acid. patients were comparable with regard to age and type of surgery. during the prophylactic treatment the effect of antibiotics used on the microbial flora of the alimentary tract was studied. patients receiving flucloxacillin ... | 1987 | 3312020 |
| [comparative in vitro study of the antimicrobial activity of ceftazidime against clinical isolates]. | the antimicrobial activity of ceftazidime was tested against 1482 gram-negative and 1216 gram-positive strains isolated from fresh clinical specimens and compared with generally used antibiotics including other third generation cephalosporins and broad spectrum penicillins. minimal inhibitory concentrations were determined in a broth dilution test on microtiter plates. in the group of the gram-negative bacteria ceftazidime was the most active of the antimicrobial agents tested with an mic of 0.5 ... | 1987 | 3312026 |
| [ceftazidime versus cefotaxime in the therapy of severe infections in intensive care patients]. | in a randomized controlled, clinical study the efficacy of ceftazidime at a dosage of 2 g b. i. d. was compared to that of cefotaxime at a dosage of 2 g t. i. d. or more in the treatment of pneumonia or peritonitis in intensive care patients. 61 of 67 assessable cases were evaluable. in the ceftazidime group ten out of 11 patients with pneumonia and 17 out of 20 with peritonitis showed a clinical success. in the cefotaxime group 15 out of 19 patients with pneumonia and eight out of 11 with perit ... | 1987 | 3312030 |
| mapping of the sor genes for l-sorbose degradation in the chromosome of klebsiella pneumoniae. | a series of mutants was isolated in klebsiella pneumoniae strain 1033, among them mutants unable to grow on l-sorbose. different r' plasmids carrying the sor genes and other surrounding chromosomal genes were also isolated. each plasmid contained the structural genes sora for an enzyme ii of the phosphoenolpyruvate-dependent carbohydrate:phosphotransferase system, sord for a d-glucitol 6-phosphate dehydrogenase, sore for an l-sorbose 1-phosphate reductase, and the corresponding regulator gene so ... | 1987 | 3312961 |
| identification of the v factor needed for synthesis of the iron-molybdenum cofactor of nitrogenase as homocitrate. | nitrogenase catalyses the atp-dependent reduction of n2 to nh3, and is composed of two proteins, dinitrogenase (mofe protein or component i) and dinitrogenase reductase (fe protein or component ii). dinitrogenase contains a unique prosthetic group (iron-molybdenum cofactor, femoco) comprised of fe, mo and s, which has been proposed as the site of n2 reduction. biochemical and genetic studies of nif- (nitrogen fixation) mutants of klebsiella pneumoniae which are defective in nitrogen fixation, ha ... | 1987 | 3313054 |
| [determination of klebsiella pneumoniae antigens in the serum of patients with bechterew's disease]. | | 1987 | 3313632 |
| [hemagglutinating and adhesive capacities of klebsiella and enterobacter strains]. | the authors analyze the data of studies on the hemagglutinating and adhesive capacity of 290 cultures, including 118 k. pneumoniae strains and 64 e. cloacae strains isolated from sick children, as well as 59 k. pneumoniae strains and 49 e. cloacae strains isolated from healthy children. the hemagglutinating properties of the strains were determined in the hemagglutination test with fresh, formalin- and tannin-treated red blood cells, the adhesive properties were studied by light microscopy. amon ... | 1987 | 3314274 |
| antimicrobial activity of tertiary amine covalently bonded to a polystyrene fiber. | tertiary amine was covalently bonded to a polystyrene fiber and examined for antibacterial activity. the tertiary amine covalently bonded to a polystyrene fiber (taf) showed a high antimicrobial activity against escherichia coli. taf exhibited a stronger antibacterial activity against gram-negative bacteria (e. coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhimurium, and serratia marcescens) than against gram-positive bacteria (staphylococcus aureus and streptococcus faecalis ... | 1987 | 3314703 |
| klebsiella pneumoniae colonization in patients with spinal cord injury. | klebsiella pneumoniae colonization of 53 patients with spinal cord injury was studied. cultures of multiple body sites from patients, the environment, food, and hospital personnel were obtained. k. pneumonaie was cultured from one or more body sites in 46% of male patients. significant bacteriuria was found in 10% of male patients. positive cultures of the urethra and perineum for k. pneumoniae were significantly associated with the use of the external condom catheter (p less than 0.05, fisher's ... | 1987 | 3315404 |
| studies on induced klebsiella mastitis with relationships among n-acetyl-beta-d-glucosaminidase, bacterial and somatic cell counts. | mastitis was induced by infusion of 3,000-7,000 colony-forming units of klebsiella pneumoniae into the left rear quarter of four primiparous holstein-freisians. greater than 100 organisms were recovered from a quarter milk sample within 2 hr and in 87.5% of the hourly cultures taken after 8 hr. bacteria, california mastitis tests, somatic cell counts and n-acetyl-beta-d-glucosaminidase levels differed significantly from that of control quarters in infused cows after hr 6, 8, 9 and 9 respectively ... | 1987 | 3315443 |
| neonatal jaundice: association with neonatal septicemia. | | 1987 | 3316017 |
| transferable resistance to third-generation cephalosporins in clinical isolates of klebsiella pneumoniae: identification of ctx-1, a novel beta-lactamase. | approximately 10% (89 isolates) of klebsiella pneumoniae isolated in 1985 from patients in intensive care units in clermont-ferrand exhibited a complex resistance phenotype towards antibiotics. they were resistant to amino-, carboxy- and ureidopenicillins, aminoglycosides (except gentamicin), chloramphenicol, sulphonamides, tetracyclines and, most importantly, to cephalosporins (except cefoxitin and latamoxef) and to aztreonam. the metabolic profile of fifty isolates was identical and seven were ... | 1987 | 3316146 |
| klebsiella pneumoniae strains more resistant to ceftazidime than to other third-generation cephalosporins. | | 1987 | 3316153 |
| rhizobium meliloti nifn (fixf) gene is part of an operon regulated by a nifa-dependent promoter and codes for a polypeptide homologous to the nifk gene product. | an essential gene for symbiotic nitrogen fixation (fixf) is located near the common nodulation region of rhizobium meliloti. a dna fragment carrying fixf was characterized by hybridization with klebsiella pneumoniae nif dna and by nucleotide sequence analysis. the fixf gene was found to be related to k. pneumoniae nifn and was therefore renamed as the r. meliloti nifn gene. upstream of the nifn coding region a second open reading frame was identified coding for a putative polypeptide of 110 amin ... | 1987 | 3316182 |
| effect of different preservative treatments on the microbial population of nigerian orange juice. | the effect of different preservative treatments on the microbial load of nigerian orange juice was studied over a period of 1 month. results obtained indicated that pasteurization at 60 degrees and 80 degrees c for 20 to 40 min, freezing at -5 degrees c, and addition of sodium benzoate at a concentration of 0.1 to 0.4% (w/v) could form a microbiological basis for the preservation of the juice for 1 month. leuconostoc mesenteroides, l. paramesenteroides, streptococcus avium, lactobacillus plantar ... | 1987 | 3316936 |
| [the role of r plasmids in the resistance of bacteria in air]. | the study of escherichia coli j 53, used as a model, has revealed that some r plasmids isolated from serratia marcescens and klebsiella pneumoniae, found to be the cause of the outbreak of hospital infection, ensure, besides multiple drug resistance, also their viability in the air. | 1987 | 3318230 |
| specific antibacterial antibody-producing cells in human nasal mucosa. | | 1987 | 3318287 |
| nitrogenase of klebsiella pneumoniae. kinetic studies on the fe protein involving reduction by sodium dithionite, the binding of mgadp and a conformation change that alters the reactivity of the 4fe-4s centre. | the kinetics of reduction of indigocarmine-dye-oxidized fe protein of nitrogenase from klebsiella pneumoniae (kp2ox) by sodium dithionite in the presence and absence of mgadp were studied by stopped-flow spectrophotometry at 23 degrees c and at ph 7.4. highly co-operative binding of 2mgadp (composite k greater than 4 x 10(10) m-2) to kp2ox induced a rapid conformation change which caused the redox-active 4fe-4s centre to be reduced by so2-.(formed by the predissociation of dithionite ion) with k ... | 1987 | 3318808 |
| [preliminary characterization of a new system allowing maltose assimilation by escherichia coli]. | we showed that klebsiella pneumoniae and erwinia herbicola possessed a pathway for maltose metabolism which could function in parallel with that encoded by the classical maltose regulon. indeed, specific dna fragments isolated from these two bacteria allowed growth on maltose of any of the mal mutants of escherichia coli when in a multicopy number. the preliminary characterization of the e. herbicola dna fragment indicated that a 4-kb region was sufficient to complement the mal mutations of e. c ... | 1986 | 3318866 |
| stimulatory effects of muramyl dipeptide upon neutrophils isolated from a local bacterial infection. | this study examined the effects of muramyl dipeptide (mdp) in vivo upon the local inflammatory response to a bacterial challenge. in addition to quantitative bacteriology of the tissues surrounding an infected suture, polymorphonuclear leucocytes (pmn) involved in the local inflammatory response were extracted and estimations made of their number, viability and phagocytic activity. fewer bacteria were recovered from the muscle around the suture in mdp-treated animals compared to placebo-treated ... | 1987 | 3318904 |
| mathematical model of klebsiella pneumoniae resistance to amikacin and gentamicin. | 1. the resistance of klebsiella pneumoniae to amikacin and gentamicin was studied by a mathematical model to predict the rate of sensitivity decrease. the results accurately matched experimental data, showing that the model is a reliable predicting tool. 2. the observations were carried out over six years and included 2677 cultures that were positive for k. pneumoniae. at the beginning of the observation period, 85.7% of the cultures were sensitive to amikacin and 40.8% were sensitive to gentami ... | 1987 | 3318975 |
| polyclonal activation of murine b cells by a membrane proteoglycan of klebsiella pneumoniae. | the lymphocyte activating properties of a membrane proteoglycan (mpg) extracted from a mutant non-encapsulated strain of klebsiella pneumoniae (kp) (biotype a i-145) were investigated. kp mpg induced a strong proliferative response of balb/c spleen cells and peyer's patches cells. thymidine incorporation was dose-related (from 1 to 100 micrograms kp mpg/ml) and reached a maximum at day 3. it was not reduced by removal of most adherent cells, nor by depletion of thy1-2 positive cells, but it was ... | 1987 | 3319300 |
| the role of capsular polysaccharide k21b of klebsiella and of the structurally related colanic-acid polysaccharide of escherichia coli in resistance to phagocytosis and serum killing. | the behaviour of strains of klebsiella aerogenes of capsular serotype k21 and strains of escherichia coli producing a structurally related polysaccharide (colanic acid) was analysed by phagocytic and serum-killing assays. the cell-surface characteristics of these strains and of non-capsulate strains derived from them were also investigated by partitioning experiments in aqueous two-polymer phase systems. the possession of k21-type capsule by k. aerogenes or colanic-acid polysaccharide by e. coli ... | 1987 | 3320374 |
| mutational analysis of upstream sequences required for transcriptional activation of the klebsiella pneumoniae nifh promoter. | upstream sequences of the klebsiella pneumoniae nifh promoter were mutagenised and activation of the mutated promoters by the nif-specific transcriptional activator protein nifa examined in vivo. of the sixteen mutations analysed, only those within the nifh upstream activator sequence (uas), characterised by a tgt-n10-aca motif, influenced nifh promoter activity. mutations altering the two-fold rotational symmetry of the uas or the spacing between the tgt and aca motifs reduced promoter activity ... | 1987 | 3320958 |
| epidemiology of infections caused by gentamicin-resistant enterobacteriaceae and pseudomonas aeruginosa over 15 years at the nashville veterans administration medical center. | nosocomial infections and gentamicin resistance were surveyed over 15 years at nashville veterans administration medical center, and trends for enterobacteriaceae and pseudomonas aeruginosa were contrasted. analysis of approximately 6,000 nosocomial infections indicated that four-fifths were caused by aerobic gram-negative bacilli. three hospital-wide outbreaks caused by enterobacteriaceae occurred; these three outbreaks were due to serratia marcescens, klebsiella pneumoniae, and enterobacter cl ... | 1987 | 3321357 |
| norfloxacin versus co-trimoxazole for treatment of urinary tract infections in adults: microbiological results of a coordinated multicentre study. | in a prospective, coordinated, double-blind multicentre trial, outpatients with urinary tract infections were randomized to 7 days b.i.d. treatment with norfloxacin 200 mg or 400 mg or trimethoprim/sulfamethoxazole. the most prevalent species was escherichia coli (76.6%) followed by staphylococcus saprophyticus (14.1%), the latter of which showed a marked seasonal variation with peak incidence during late summer. minimum inhibitory concentrations (mics) of 11 antibiotics for 651 pre-treatment ba ... | 1987 | 3321412 |
| [infectious pathology in full-term neonates with a low birth weight for their gestational age]. | a retrospective study of infectious diseases has been performed in 194 small-for-date full-term babies, finding septicaemia in 7.2% of cases, urinary tract infection in 5.2%, meningoencephalitis in 1% and enterocolitis in 3%. most frequent infections agents were: 1) in septicaemia, klebsiella pneumoniae (28.5%), e. coli and staphylococcus coagulase negative (21.4% for each); 2) in urinary tract infections, klebsiella pneumoniae and e. coli (40% for each); 3) in meningoencephalitis, klebsiella pn ... | 1987 | 3322124 |
| heterogeneity of genes conferring high-level resistance to erythromycin by inactivation in enterobacteria. | we have constructed a probe specific for the gene erea of plasmid pip1100, which confers high-level resistance to erythromycin in escherichia by production of an erythromycin esterase. the distribution of the gene erea in enterobacteria highly resistant to erythromycin isolated from human faeces was studied by colony hybridization. the gene erea was detected in strains of e. coli belonging to various biotypes, in klebsiella pneumoniae, in enterobacter agglomerans and in one strain of a "coliform ... | 1986 | 3322168 |
| a quantitative approach to sequence comparisons of nitrogenase mofe protein alpha- and beta-subunits including the newly sequenced nifk gene from klebsiella pneumoniae. | the nucleotide sequence was determined for part of the klebsiella pneumoniae nif gene cluster containing the 3' end of the nifd gene and the entire length of the nifk gene (encoding the alpha- and beta-subunits of the nitrogenase mofe protein respectively), as well as the putative start of the nify gene, a gene of as yet unknown function. a broad-based comparison of a number of mofe protein alpha-subunits, beta-subunits and alpha-versus beta-subunits was carried out by the use of a computer prog ... | 1987 | 3322261 |
| nucleotide sequence of the klebsiella pneumoniae nifd gene and predicted amino acid sequence of the alpha-subunit of nitrogenase mofe protein. | the nucleotide sequence of the klebsiella pneumoniae nifd gene is presented and together with the accompanying paper [holland, zilberstein, zamir & sussman (1987) biochem. j. 247, 277-285] completes the sequence of the nifhdk genes encoding the nitrogenase polypeptides. the k. pneumoniae nifd gene encodes the 483-amino acid-residue nitrogenase alpha-subunit polypeptide of mr 54156. the alpha-subunit has five strongly conserved cysteine residues at positions 63, 89, 155, 184 and 275, some occurri ... | 1987 | 3322262 |
| klebsiella pneumoniae nitrogenase. inhibition of hydrogen evolution by ethylene and the reduction of ethylene to ethane. | ethylene (c2h4) inhibited h2 evolution by the mo-containing nitrogenase of klebsiella pneumoniae. the extent of inhibition depended on the electron flux determined by the ratio of fe protein (kp2) to mofe protein (kp1) with kic2h4 = 409 kpa ([kp2]/[kp1] = 22:1) and kc2h4i = 88 kpa ([kp1]/[kp2] = 21:1) at 23 degrees c at ph 7.4. at [kp2]/[kp1] = 1:1, inhibition was minimal with c2h4 (101 kpa). extrapolation of data obtained when c2h4 was varied from 60 to 290 kpa indicates that at infinite pressu ... | 1987 | 3322266 |
| structure of the capsular polysaccharide of escherichia coli o9:k32(a):h19. | the capsular polysaccharide of the bacterium escherichia coli o9:k32(a):h19 was analyzed using chemical methods (hydrolysis, sequential smith degradation, methylation analysis) together with 1h- and 13c-n.m.r. spectroscopy. 13c-n.m.r. spectroscopy and chemical analyses indicated that the k32 polysaccharide is composed of equimolar proportions of glucose, galactose, rhamnose, and glucuronic acid, and carries o-acetyl groups. 1h-n.m.r. analysis of native k32 polysaccharide revealed five resonances ... | 1987 | 3322556 |
| cloning and expression in escherichia coli of the klebsiella pneumoniae genes for production, surface localization and secretion of the lipoprotein pullulanase. | this article describes the reconstitution in escherichia coli of a heterologous protein secretion system comprising a gene for an extracellular protein together with its cognate secretion genes. the protein concerned, pullulanase, is a secreted lipoprotein of the gram-negative bacterium klebsiella pneumoniae. it is initially localized to the cell surface before being specifically released into the medium. e. coli carrying the cloned pullulanase structural gene (pula) produces pullulanase but doe ... | 1987 | 3322811 |
| difference of virulence in causing metritis in horses between heavily encapsulated, less heavily encapsulated and non-capsulated strains of klebsiella pneumoniae capsular type 1. | | 1987 | 3323592 |
| positional requirements for the function of nif-specific upstream activator sequences. | the upstream activator sequence (uas) found in klebsiella pneumoniae nif promoters and required for the activation of transcription by nifa, is absent from the niff-nifl intergenic region, but is present downstream from the nifla transcription start at +59. to determine whether nif upstream activator sequences can function in a 3' position, the nifh uas was cloned downstream from the nifh transcription start, but no activation of transcription by nifa dependent upon the uas in its 3' location co ... | 1987 | 3323835 |
| capsule types of klebsiella pneumoniae isolated from the genital tract of mares with metritis, extra-genital sites of healthy mares and the genital tract of stallions. | a survey of k. pneumoniae was performed on cervical swabs, feces and nasal swabs of mares and on samples from the genital tract of stallions from 1980 to 1986 in south-western hokkaido, japan. k1 was the predominant type (79 of 88, 89.8%) in the metritis cases due to k. pneumoniae in mares of racing breeds. the same type was isolated from semen and swabs of the fossa glandis of 6 of 20 (30.0%) of the stallions of racing breeds. heavily encapsulated and less heavily encapsulated k1 strains were i ... | 1987 | 3324458 |
| growth inhibition of escherichia coli and klebsiella pneumoniae during involution of the bovine mammary gland: relation to secretion composition. | mammary secretions from 12 holstein dairy cows were collected to evaluate growth inhibition of escherichia coli and klebsiella pneumoniae during involution and during physiologic transitions of the mammary gland. mammary secretions obtained during late lactation poorly inhibited growth of e coli and k pneumoniae. however, as involution progressed, mammary secretions increasingly inhibited growth of both coliform mastitis pathogens. greatest inhibition of e coli and k pneumoniae growth was observ ... | 1987 | 3324839 |
| klebsiella pneumoniae c3 lipopolysaccharide mutants obtained by resistance to bacteriophage fc3-9. | bacteriophage fc3-9 is one of the several bacteriophages of klebsiella pneumoniae c3 isolated in our laboratory. mutants resistant to this bacteriophage were isolated and found to be devoid of lipopolysaccharide o antigen, modified in outer-membrane protein composition and sensitive to complement killing (serum-sensitive), unlike the wild-type strain. serum-resistant mutants were isolated from these strains. they regained the lipopolysaccharide o antigen and the wild-type outer membrane protein ... | 1986 | 3324942 |
| comparative activities of ciprofloxacin and ceftazidime against klebsiella pneumoniae in vitro and in experimental pneumonia in leukopenic rats. | the antibacterial activities of ciprofloxacin and ceftazidime against klebsiella pneumoniae in vitro and in vivo were compared. although there was only a minor difference between the mbcs of both drugs, the bacterial killing rate of ciprofloxacin in vitro was very fast in comparison with that of ceftazidime. similarly, the intravenous administration of ciprofloxacin at 1 h after bacterial inoculation resulted in effective bacterial killing in the lungs of leukopenic rats. this killing was dose d ... | 1987 | 3324962 |
| metabolism of 1-aminoethylphosphinate generates acetylphosphinate, a potent inhibitor of pyruvate dehydrogenase. | the alanine analogue 1-aminoethylphosphinate [h3c-ch(nh2)-po2h2] effectively inhibited anthocyanin synthesis in buckwheat hypocotyls and caused an increase in the concentrations of alanine and alanine-derived metabolites. aminotransferase inhibitors partially alleviated the effects of the analogue. 1-aminoethylphosphinate did not affect the growth of klebsiella pneumoniae under anaerobic conditions, but under aerobic conditions it inhibited growth and caused the massive excretion of pyruvate. th ... | 1987 | 3325039 |
| ofloxacin in respiratory tract infection. a review of the results of clinical trials in japan. | an open clinical study of ofloxacin in respiratory tract infections was conducted with patients receiving daily doses of ofloxacin 300 mg, 400 mg or 600 mg. the duration of treatment was 6 to 14 days for 70% of the patients. ofloxacin was effective in 668 of 828 patients analysed (80.7%). of 293 patients with upper respiratory infections, the efficacy rate was 85.3%. in 535 cases with lower respiratory infections, ofloxacin was effective in 78.1%. it is noteworthy that a 70% efficacy rate was ob ... | 1987 | 3325261 |
| chromotropic character of bacterial acidic polysaccharides: part i--induction of metachromasia in dye neutral red. | | 1987 | 3325406 |
| microbiological perspectives of co-trimoxazole. | trimethoprim-sulfamethoxazole in vitro activity was compared with ampicillin, tetracycline, sulfonamide and trimethoprim against isolates of 24 gram-negative and 11 gram-positive species. the incidence of more than 10% of strains with minimal inhibitory concentrations above 32 mg/l was restricted to escherichia coli, shigella spp., klebsiella pneumoniae, providencia rettgeri, morganella morganii, methicillin-resistant staphylococcus aureus and staphylococcus epidermidis. occasionally, streptococ ... | 1987 | 3325434 |
| acute myocarditis in association with klebsiella pneumonia. | | 1987 | 3325495 |
| iron and immunologic function. | this short review focuses on the immunological functions of iron-containing compounds. five such compounds--transferrin, haptoglobin, hemopexin, albumin, and lactoferrin--are thought to contribute to normal infection resistance. experimental results that demonstrate the ability of these compounds to inhibit the growth of an important pathogen, klebsiella pneumoniae, are discussed. | 1987 | 3325510 |
| influence of heavy metals on the resistance of mice toward infection. | previous studies have shown that heavy metals may exert marked immunomodulatory effects, at least in rodents, despite some discrepancies. however, the mechanism of their influence on the immune system is still unclear. as host resistance assays against experimental infections are generally considered as the most relevant criteria when predicting the immunotoxicity of drugs and chemicals, the effects of lead acetate, nickel chloride and sodium selenite on the resistance toward experimental klebsi ... | 1987 | 3325545 |
| influence of dose frequency on the therapeutic efficacies of ciprofloxacin and ceftazidime in experimental klebsiella pneumoniae pneumonia and septicemia in relation to their bactericidal activities in vitro. | the antibacterial activities of ciprofloxacin versus ceftazidime against klebsiella pneumoniae in vitro and in vivo were compared. although there was only a minor difference in mbc values between both drugs ciprofloxacin demonstrated a high and dose-dependent bacterial killing rate in vitro and in lungs of leukopenic rats in contrast to the more time-dependent bactericidal activity of ceftazidime. after treatment of a k.pneumoniae pneumonia and septicemia the efficacy of ciprofloxacin was only s ... | 1987 | 3325928 |
| antibiotic resistance and plasmid profiles of escherichia coli and klebsiella isolated from in-patients receiving prolonged antibiotic therapy. | distribution by serogroup, phage type, colicin production, colicin type, sensitivity to antibiotics and plasmid characteristics of 74 escherichia coli and 11 klebsiella strains isolated from hospitalized patients receiving prolonged antibiotic therapy indicated that the infections were not associated with the hospital environment. resistance was tested to 26 antibiotics, some of them being not generally used in therapy; 30 strains were resistant to 4 to 17 antibiotics. there was a significant di ... | 1987 | 3326388 |
| antibody response to bacterial antigens covalently bound to biodegradable polymerized serum albumin beads. | model vaccines have been made by covalently linking clostridium botulinum type d toxin and klebsiella pneumoniae capsular polysaccharide antigen to polymerized rabbit serum albumin beads. when injected into rabbits these bead vaccines induced an enhanced production of specific humoral antibody without causing adverse reactions. the adjuvant effect is due to a slow release from the bead structure and offers an alternative to oil emulsions and mineral salt adsorbents. | 1987 | 3326866 |
| effect of ceftriaxone-induced alterations of bacteria on neutrophil bactericidal function. | two bacterial strains (staphylococcus aureus and klebsiella pneumoniae) were exposed to subinhibitory concentrations of ceftriaxone. after an overnight culture in presence of 1 mic of ceftriaxone either in broth or on solid medium s. aureus showed enlarged forms which were better phagocytosed (increase about 40%) and killed (increase about 50%) than control staphylococci. exposure of k. pneumoniae to 0.1 mic ceftriaxone resulted in filamentation of bacteria. when grown in the presence of 0.01 mi ... | 1987 | 3326873 |
| novel beta-lactamase in a clinical isolate of klebsiella pneumoniae conferring unusual resistance to beta-lactam antibiotics. | | 1987 | 3326875 |
| adherence of klebsiella to human buccal epithelial cells. | | 1987 | 3327821 |
| susceptibility of klebsiella pneumoniae to heavy metal ions in vitro. | | 1987 | 3327822 |
| a rapid and efficient method for plasmid transformation of klebsiella pneumoniae and escherichia coli. | a rapid and efficient method for plasmid transformation of klebsiella pneumoniae m5a1 and escherichia coli k12 has been developed. the method, which uses a freeze-thaw cycle in the presence of cacl2 to facilitate dna uptake, is substantially more efficient for k. pneumoniae m5a1 than the conventional transformation procedure for e. coli. the simplicity and speed of the method makes it very attractive for routine transformation of k. pneumoniae m5a1 and e. coli k12. | 1987 | 3327913 |
| [laboratory and clinical studies of flomoxef]. | laboratory and clinical studies were performed on a new oxacephem antibiotic, flomoxef (fmox, 6315-s). in vitro antibacterial activity of fmox was evaluated in comparison to latamoxef (lmox), cefmetazole (cmz), cefazolin (cez) using clinically isolated strains of gram-negative and gram-positive bacteria. antibacterial activities of fmox were stronger than lmox, cmz, cez against escherichia coli, klebsiella but only slightly effective against staphylococcus aureus. this antibiotic drug was admini ... | 1987 | 3327964 |
| [the antimicrobial activity of sisomicin against fresh clinical isolates]. | antimicrobial activities of sisomicin (siso) against clinical isolates obtained in the second half of 1986 were investigated together with other 4 aminoglycosides (ags) (gentamicin (gm), tobramycin (tob), dibekacin (dkb), amikacin (amk] and 2 cephems (cefotiam, cefotaxime), and were compared to the results reported in the period of late 1970's through early 1980's in japan. 1. the incidence of siso-resistant staphylococcus aureus in the present study was 18% and is comparable to that of the othe ... | 1987 | 3327965 |
| [clinical laboratory approach for estimating the effective administrative dose of latamoxef. significance of a 4-category system interpretation of the latamoxef disc susceptibility test]. | in vitro activities of latamoxef (lmox) against 249 clinical isolates were determined using the agar dilution method at an inoculum level of 10(6) cfu/ml. lmox was highly active against escherichia coli, klebsiella pneumoniae, proteus mirabilis (except 2 out of 30 strains) and proteus vulgaris with mic values below 3.13 micrograms/ml. it was also active against enterobacter aerogenes with mic80 of 1.56 micrograms/ml. lmox was less active against serratia marcescens, inhibiting about 43% of strai ... | 1987 | 3327966 |
| [susceptibility of gram-negative bacilli to aminoglycosides]. | in this study, the antibiotic sensitivity results of some gram-negative bacilli to aminoglycosides have been presented. 704 e. coli, 152 klebsiella, 158 proteus, 155 pseudomonas strains were examined. these bacilli were isolated from the clinical samples at the bacteriology lab.in ibni sina hospital, between january 1986-january 1987. the percents of e. coli strains susceptible to gentamicin, tobramycin, netilmicin and amikacin were 74%, 84%, 90%, 85% respectively. the sensitivity pattern of pro ... | 1987 | 3328818 |
| qualitative evidence for expression of klebsiella pneumoniae nif in pseudomonas putida. | pseudomonas putida mt20-3 carrying the klebsiella pneumoniae nif plasmids prd1 or pmf250 showed highly o2-sensitive aerobic acetylene reduction on low-n pyruvate or glucose agar. this finding confirms unequivocally that k. pneumoniae nif can be expressed in an obligate aerobe. | 1987 | 3329216 |
| analysis of the klebsiella pneumoniae ntrb gene by site-directed in vitro mutagenesis. | a number of in-frame insertion and deletion mutations have been constructed in vitro in the klebsiella pneumoniae ntrb gene and the effects of each mutant ntrb protein on ntrc activity have been assessed after reintroduction of the ntrb mutation into the glna ntrbc operon. these experiments suggest that the phosphorylation of ntrc catalysed by ntrb not only makes ntrc competent as a transcriptional activator but also improves the dna-binding properties and hence the negative control functions of ... | 1987 | 3329695 |
| a gene fusion approach to the study of pullulanase export and secretion in escherichia coli. | a series of fusions between the gene for the klebsiella pneumoniae secreted lipoprotein pullulanase (pula) and the genes for cytoplasmic beta-galactosidase (lacz) or periplasmic alkaline phosphatase (phoa) were created by transposon mutagenesis using mini-mudii1681 or tnphoa, respectively. the hybrid genes were expressed in escherichia coli k-12 with or without the k. pneumoniae genes that promote pullulanase secretion in e. coli. we characterized seven different pula-lacz gene fusions encoding ... | 1987 | 3329697 |
| ultrasound-guided brain abscess aspiration in neonates. | four cases of brain abscess in neonates are described, diagnosed by ultrasonography and ct. all abscesses were confirmed surgically. one patient was operated on 5 weeks after diagnosis because of initial parental refusal. the etiology in all cases was meningitis superimposed on an hypoxic-ischemic insult. two cases had a single abscess while the other two had multiple lesions. all cases were operated on with intraoperative ultrasound examination through the fontanelle. the case with delayed aspi ... | 1987 | 3329960 |
| [bactericidal activity of disinfectants against certain bacterial strains. klebsiella pneumonia]. | twenty disinfectants were tested against a strain of k. pneumoniae. a suspension test with incremental addition of microorganisms was used. the influence of interfering substances (proteins and hard water) on the activity of disinfectants was also determined. iodine compounds were influenced by proteins in distilled and hard water. the presence of mineral ions inactivated the polyvinylpyrrolidone-iodine 10% and chloroxylenol preparations against k. pneumoniae. the activity of some iodine product ... | 1987 | 3329985 |
| [experimental cystitis in the rat and the therapeutic effect of a single dose of fosfomycin trometamol]. | | 1987 | 3330644 |
| requirements for transcriptional activation in vitro of the nitrogen-regulated glna and nifla promoters from klebsiella pneumoniae: dependence on activator concentration. | three proteins involved in nitrogen regulation in klebsiella pneumoniae, ntra, ntrb and ntrc, have been purified. in a defined in vitro system all three ntr proteins are required for initiation of transcription at the ntr activatable glna and nifla promoters. however, in crude s-30 extracts, transcription from the glna promoter, but not the nifla promoter, can be activated in the absence of ntrb. a higher concentration of ntrc is required for activation of nifla transcription than for glna trans ... | 1987 | 3330758 |
| imipenem/cilastatin therapy for serious infections in neonates and infants. | sixty-one hospitalized infants aged one day to six months were enrolled in an open, multicenter noncomparative clinical study of the efficacy and safety of imipenem/cilastatin. patients weighing less than 1500 g (four males/ten females, group 1) and those greater than or equal to 1500 g (31 males/16 females, group 2) were analyzed separately. total daily dose (divided into b.i.d. (27) or t.i.d. (34) regimens) ranged from 50 to 101.4 mg/kg given for 10.8 days (means, range 2 to 35 days) for group ... | 1987 | 3331042 |
| imipenem/cilastatin for pediatric infections in hospitalized patients. | one hundred and four children who were hospitalized for documented or suspected non-cns bacterial infections (56 males/48 females, 22 days to 15 years old) were treated with intravenous imipenem/cilastatin for 9.4 days (range 3 to 28 days). children up to three years of age received 100 mg/kg/day and older children 60 mg/kg/day, administered in four divided doses. bacterial pathogens were isolated before therapy in 85%. diagnoses in the 74 evaluable patients included bronchopneumonia with or wit ... | 1987 | 3331043 |
| [determination of the bactericidal potency of cefatrizine and other oral antibiotics against haemophilus influenzae, klebsiella pneumoniae and streptococcus pyogenes]. | the in vitro determination of the t log and the minimal bactericidal time of cefatrizine was evaluated against h. influenzae, str. pyogenes a isolated from clinical specimens. and k. pneumonia atcc 10031 and compared with that of amoxycillin, cefaclor, cefroxadine and miocamycin against the same bacterial strains. cefatrizine demonstrate the shorter tmb against all bacterial strains and antibiotic tested with mbcs. | 1986 | 3331159 |
| studies on bacteriophage penetration in patients subjected to phage therapy. | two healthy volunteers and 56 patients with suppurative bacterial infections were tested for penetration of oral administered bacteriophage to the blood circulation system and urinary tract. in the blood and urine samples collected from patients before phage therapy application, no presence of "wild phages" was confirmed. examination performed on the 10th day of phage therapy revealed the presence of bacteriophages in 47 of 56 blood samples tested; positive result of examination was obtained in ... | 1987 | 3332066 |
| [the role of ofloxacin (tarivid) therapy in respiratory and urinary tract infections]. | in this study, 14 patients with urinary tract infection and 8 patients with respiratory tract infection were given 400 mg/day of ofloxacin. 12 of 14 patients with urinary tract infection and 4 of 8 patients with respiratory tract infection was cured and clinically improved. 12 of 13 e. coli isolates were eradicated with this treatment. no severe adverse reactions were observed. no in vitro resistance against ofloxacin was seen. | 1987 | 3332699 |
| [susceptibility of some bacteria isolated from clinical samples to third-generation cephalosporins]. | in this study, we evaluated the susceptibility of some staph. aureus, staph. epidermidis, e. coli, klebsiella, proteus, pseudomonas strains to third-generation cephalosporins in-vitro by disk diffusion technique which are in use in turkey. these bacteria isolated from various clinical samples at the bacteriology laboratory in ibni sina hospital between january 1986-january 1987. the antibiotics used in this study are ceftizoxime, cefotaxime, ceftriaxone, cefoperazone. according to the results of ... | 1987 | 3332704 |
| [a comparison of the in vitro susceptibility of three bacteria to beta-lactamase inhibitors and to ampicillin and cefazolin]. | in this study in vitro susceptibility of three different bacteria, which are able to release various beta-lactamases, against combinations of clavulanic acid + amoxicillin and sulbactam + ampicillin were compared with susceptibility to ampicillin alone and cefazolin of same strains. | 1987 | 3332705 |
| ten-year survey of positive blood cultures among admissions to a neonatal intensive care unit. | a 10-year survey of positive blood cultures was conducted among 6,616 infants admitted to an intensive care unit from 1974-1983. group b streptococci (59) and e. coli (32) were the most common pathogenic organisms isolated from the early onset group (less than 72 hours of age). the percentage of infants with bacteremia, the birth weight distribution of infected infants, and the organisms isolated did not change in the early onset group throughout the study period. coagulase negative staphylococc ... | 1987 | 3333254 |
| bacteremia in adult men. | a prospective study was done to characterize positive blood cultures in adult male veterans. the research included 277 patients over a 15-month period. there were 348 organisms isolated, of which 65 percent were pathogens, 6 percent probable pathogens, 4 percent probable contaminants, and 25 percent contaminants. the most common isolates were coagulase-negative staphylococci (23 percent), escherichia coli (12 percent), klebsiella pneumoniae (10 percent), staphylococcus aureus (9 percent), strept ... | 1987 | 3334058 |
| [polybiotrophy of bacteria of the genus klebsiella]. | | 1987 | 3334268 |
| immunomodulating properties of newer cephalosporins: a preliminary classification. | | 1987 | 3334561 |
| effect of ceftriaxone on bacterial killing by human neutrophils in vitro. | | 1987 | 3334563 |
| interaction of cefotaxime versus desacetyl cefotaxime on human neutrophil functions. | | 1987 | 3334564 |
| pharmacokinetic studies in rats with ofloxacin and mf-934, a new fluorinated quinolone. | | 1987 | 3334569 |
| intracavitary mass in a patient with klebsiella pneumonia. | | 1988 | 3335151 |
| presence of langerhans cells in the cornea of klebsiella keratoconjunctivitis mice. | frozen sections of normal balb/c corneas and corneas from balb/c mice with klebsiella keratoconjunctivitis were examined for the expression of class i, class ii h-2 antigens and mac-1 antigens using monoclonal antibodies in an immunoperoxidase technique. class i antigens were readily detected, in both normal and diseased corneas, mainly in the epithelium. class ii (ia) and mac-1 antigens were not detected in the normal corneas. however, these two antigens were found mainly in the epithelium and ... | 1988 | 3335423 |
| [nosocomial infections of the urinary tract in urology patients]. | researches effected in different nations have revealed that 5-12% of all hospitalized patients was affected with a nosocomial infection and that almost 1/3 of these were urinary tract infections. the hospitalized urological patients have a considerable proneness to contract a nosocomial urinary tract infection, because they are subjected to transurethral or suprapubic bladder catheterisms, to transurethral surgery and to the endoscopies, that are promoting maneuvers the urinary tract infections. ... | 1988 | 3340313 |
| the microbiology of neonatal peritonitis. | to review the bacteriology of neonatal intra-abdominal sepsis, we reviewed peritoneal cultures from 86 newborns undergoing operation for necrotizing enterocolitis (nec) for the type and incidence of microorganism recovered. as a control, we conducted a similar review in 59 children with perforated appendicitis during the same period. necrotizing enterocolitis was characterized by a lower incidence of polymicrobial contamination (1.7 organisms per patient vs 2.4 organisms per patient, nec vs appe ... | 1988 | 3341903 |
| septicemia from biliary tract infection. | one hundred four strains of microorganisms were isolated from the blood in 76 episodes of septicemia originating from biliary tract infection. the 70 patients involved included 40 with acute cholecystitis without previous surgery, 17 with cholangitis following previous surgery, and 13 patients with malignant disease, with or without previous surgery. the most common organisms were escherichia coli, klebsiella, streptococcus, and proteus. various streptococci, most of them from group d, were invo ... | 1988 | 3341915 |
| stopcock-induced bacterial contamination in the infusion system. | | 1988 | 3342639 |
| bronchoscopic protected catheter brush for the diagnosis of pulmonary infections. | a new bronchoscopic-protected catheter brush (bpcb), designed to obtain uncontaminated bronchial secretions, was studied in vitro and in vivo. the device was composed of a standard biopsy brush, protected by a single catheter and occluded with an agar plug. ejection of the plug was obtained neither by advancing the brush nor by advancing an inner cannula (as in a telescoping catheter brush), but instead, by an air flux, provided by a syringe which was connected to the proximal tip. in the first ... | 1988 | 3349830 |
| nosocomial bacteremia in a large spanish teaching hospital: analysis of factors influencing prognosis. | five hundred forty-three episodes of nosocomial bacteremia were prospectively followed in a large spanish university hospital. the commonest isolates were staphylococcus epidermidis, staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, and candida species. the most frequent sources of infection were intravenous lines, urinary tract, and lower respiratory tract. overall mortality was 18%. a step-forward logistic regression analysis defined eight variables independently influencing the ... | 1988 | 3353630 |