| comparative study of diagnostic accuracy of established pcr assays and in-house developed sdaa pcr method for detection of mycobacterium tuberculosis in symptomatic patients with pulmonary tuberculosis. | indian contribution to global burden of tuberculosis is about 26%. in the present study we have developed an in-house pcr assay using primers for sdaa gene of mycobacterium tuberculosis and evaluated against already established primers devr, is6110, mpb64, rpob primers for diagnosis of pulmonary tuberculosis. | 2013 | 23867867 |
| validating a breath collection and analysis system for the new tuberculosis breath test. | mycobacterium tuberculosis releases four volatile compounds, methyl phenyl-acetate, methyl nicotinate, methyl p-anisate and o-phenylanisole, some of which we have previously been reported to be detected in the breath of tuberculosis patients (syhre et al 2009 tuberculosis 89 263-6). these volatiles have the potential to offer a non-invasive and sensitive breath test for the detection of tuberculosis infection. to determine the best sample collection and pre-concentration system a number of varia ... | 2013 | 23867751 |
| over-expression of gene encoding heat shock protein 70 from mycobacterium tuberculosis and its evaluation as vaccine adjuvant. | heat shock proteins (hsps) are evolutionary ancient and highly conserved molecular chaperons found in prokaryotes as well as eukaryotes. hsp70 is a predominant member of hsp family. microbial hsp70s (mhsp70s) have acquired special significance in immunity since they have been shown to be potent activators of the innate immune system and generate specific immune responses against tumours and infectious agents. | 2013 | 23867667 |
| rapid identification of clinical mycobacterial isolates by protein profiling using matrix assisted laser desorption ionization-time of flight mass spectrometry. | the purpose of this study was to evaluate the identification of mycobacterium tuberculosis which is often plagued with ambiguity. it is a time consuming process requiring 4-8 weeks after culture positivity, thereby delaying therapeutic intervention. for a successful treatment and disease management, timely diagnosis is imperative. we evaluated a rapid, proteomic based technique for identification of clinical mycobacterial isolates by protein profiling using matrix assisted laser desorption ioniz ... | 2013 | 23867666 |
| early secreted antigenic target of 6 kda (esat-6) protein of mycobacterium tuberculosis induces interleukin-8 (il-8) expression in lung epithelial cells via protein kinase signaling and reactive oxygen species. | early secreted antigenic target of 6 kda (esat-6) of mycobacterium tuberculosis is critical for the virulence and pathogenicity of m. tuberculosis. il-8, a major chemotactic cytokine for neutrophils and t lymphocytes, plays important roles in the development of lung injury. to further understand the role of esat-6 in lung pathology associated with tuberculosis development, we studied the effects of esat-6 on the regulation of il-8 expression in lung epithelial cells. esat-6 induced il-8 expressi ... | 2013 | 23867456 |
| combination of multiplex pcr with denaturing high-performance liquid chromatography for rapid detection of mycobacterium genus and simultaneous identification of the mycobacterium tuberculosis complex. | a new assay with the combination of multiplex polymerase chain reaction and denaturing high-performance liquid chromatography analysis was developed for simultaneous detection of mycobacterium genus and identification of the mycobacterium tuberculosis complex (mtc). targeting at genus-specific 16s rrna sequence of mycobacterium and specific insertion elements is6110 and is1081 of mtc, the assay was validated with 84 strains covering 23 mycobacteria species and 30 strains of non-mycobacteria spec ... | 2013 | 23867326 |
| synthesis and structure activity relationship of imidazo[1,2-a]pyridine-8-carboxamides as a novel antimycobacterial lead series. | imidazo[1,2-a]pyridine-8-carboxamides as a novel antimycobacterial lead were generated by whole cell screening of a focused library against mycobacterium tuberculosis. herein, we describe the synthesis and structure activity relationship evaluation of this class of inhibitors and the optimization of physicochemical properties. these are selective inhibitors of mycobacterium tuberculosis with no activity on either gram positive or gram negative pathogens. | 2013 | 23867166 |
| [effects of exogenous interleukin-2, interleukin-23 on differentiation of irbp 1-20-specific t cells toward th1 and th17 and comparison of the pathogenicity of irbp 1-20-specific t cells]. | to explore the effects of exogenous interleukin (il)-2 and il-23 on differentiation of irbp 1-20-specific t cells originated from c57bl/6 mouse with experimental autoimmune uveitis (eau) and to investigate the difference in pathogenicity. | 2013 | 23866703 |
| the beginnings of modern research on propolis in poland. | propolis studies in poland were initiated by professor stan scheller in the 1960s. it was a team of polish researchers who developed a method of introducing hydrophobic ethanol extracts of propolis (eep) into aqueous solutions, which enabled the study of their biological properties. the studies performed in poland showed that eep possesses antioxidant, radioprotective, and immunostimulating properties. it was possible to demonstrate antibacterial activity of propolis on gram-positive bacteria, v ... | 2013 | 23864903 |
| macrophages in tuberculosis: friend or foe. | tuberculosis (tb) remains one of the greatest threats to human health. the causative bacterium, mycobacterium tuberculosis (mtb), is acquired by the respiratory route. it is exquisitely human adapted and a prototypic intracellular pathogen of macrophages, with alveolar macrophages (ams) being the primary conduit of infection and disease. the outcome of primary infection is most often a latently infected healthy human host, in whom the bacteria are held in check by the host immune response. such ... | 2013 | 23864058 |
| mycobacterium tuberculosis... can we beat it? report from a euroscicon conference 2013. | | 2013 | 23863609 |
| uv-fixed-thick-blotch preparation improves sensitivity of auramine staining. | we describe here a very simple modification of the auramine staining procedure based on preparation of a uv-fixed thick blotch which allowed us to reach an overall sensitivity of 0.82 (592 acid-fast bacillus [afb]-positive specimens/722 initial respiratory specimens with positive mycobacterial culture) and sensitivities of 0.93 (526 afb-positive specimens/564 culture-positive specimens) for mycobacterium tuberculosis complex and 0.42 (66 afb-positive specimens/158 culture-positive specimens) for ... | 2013 | 23863571 |
| evaluation of the bd bactec mgit 320 system for detection of mycobacteria and drug susceptibility testing of mycobacterium tuberculosis. | two different laboratories evaluated growth and detection of mycobacteria and drug susceptibility testing of mycobacterium tuberculosis by the bd bactec mgit 320 using the bd bactec mgit 960 (bd diagnostics, sparks, md) as a reference method. out of 359 processed sputum specimens for detection of mycobacteria, 99.7% were in agreement between the mgit 320 and mgit 960. streptomycin (str), isoniazid (inh), rifampin (rif), ethambutol (emb) (collectively known as sire), and pyrazinamide (pza) drug s ... | 2013 | 23863564 |
| comparison of the xpert mtb/rif and cobas taqman mtb assays for detection of mycobacterium tuberculosis in respiratory specimens. | the xpert mtb/rif assay (cepheid, sunnyvale, ca) is a fully automated, cartridge-based real-time pcr assay designed to detect mycobacterium tuberculosis and rifampin resistance within 2 h. the performance of the xpert assay has been evaluated in various clinical settings. however, there are few data comparing the xpert assay to the cobas taqman mtb test (roche diagnostics, basel, switzerland), one of the most widely utilized molecular assays for m. tuberculosis detection. in this prospective stu ... | 2013 | 23863563 |
| bcg vaccine in korea. | the anti-tuberculosis bacille de calmette et guérin (bcg) vaccine was developed between 1905 and 1921 at pasteur institutes of lille in france, and was adopted by many countries. bcg strains comprise natural mutants of major virulence factors of mycobacterium tuberculosis and that bcg sub-strains differ markedly in virulence levels. the tuberculosis became endemic in korea after the korean war (1950s). the bcg strain, which was donated by pasteur institutes, was brought to korea in 1955, and the ... | 2013 | 23858398 |
| performance comparison between the mycobacteria growth indicator tube system and löwenstein-jensen medium in the routine detection of mycobacterium tuberculosis at public health care facilities in rio de janeiro, brazil: preliminary results of a pragmatic clinical trial. | in view of the fact that the world health organization has recommended the use of the mycobacteria growth indicator tube (mgit) 960 system for the diagnosis of tuberculosis and that there is as yet no evidence regarding the clinical impact of its use in health care systems, we conducted a pragmatic clinical trial to evaluate the clinical performance and cost-effectiveness of the use of mgit 960 at two health care facilities in the city of rio de janeiro, brazil, where the incidence of tuberculos ... | 2015 | 23857683 |
| pharmacokinetic and in vivo efficacy studies of the mycobactin biosynthesis inhibitor salicyl-ams in mice. | mycobactin biosynthesis in mycobacterium tuberculosis facilitates iron acquisition, which is required for growth and virulence. the mycobactin biosynthesis inhibitor salicyl-ams [5'-o-(n-salicylsulfamoyl)adenosine] inhibits m. tuberculosis growth in vitro under iron-limited conditions. here, we conducted a single-dose pharmacokinetic study and a monotherapy study of salicyl-ams with mice. intraperitoneal injection yielded much better pharmacokinetic parameter values than oral administration did. ... | 2013 | 23856770 |
| analysis of protein species differentiation among mycobacterial low-mr-secreted proteins by narrow ph range immobiline gel 2-de-maldi-ms. | secreted proteins of bacteria are preferentially capable of interacting with host cells and are therefore of special biological and medical interest. narrow ph range 2-de and maldi-toftof-ms combine high-resolution protein separation with highly sensitive identification of proteins. secreted proteins of mycobacterium tuberculosis were separated at the protein species level, distinguishing different protein species of one protein. we focused on the pi range 4.0-4.7 and the mr range 6-20kda of the ... | 2014 | 23856608 |
| [detection of pnca mutation associated with pyrazinamide resistance in mycobacterium tuberculosis by high-resolution melting cure analysis]. | to evaluate the application of a high-resolution melting (hrm) curve analysis for rapid detection of pyrazinamide resistance in mycobacterium tuberculosis (m. tuberculosis). | 2013 | 23856143 |
| enhanced innate immunity as explanation for reduced mycobacterium tuberculosis infection in bacillus calmette-guérin-immunized children. | | 2013 | 23855702 |
| irf8-regulated genomic responses drive pathological inflammation during cerebral malaria. | interferon regulatory factor 8 (irf8) is required for development, maturation and expression of anti-microbial defenses of myeloid cells. bxh2 mice harbor a severely hypomorphic allele at irf8 (irf8(r294c)) that causes susceptibility to infection with intracellular pathogens including mycobacterium tuberculosis. we report that bxh2 are completely resistant to the development of cerebral malaria (ecm) following plasmodium berghei anka infection. comparative transcriptional profiling of brain rna ... | 2013 | 23853600 |
| b cells regulate neutrophilia during mycobacterium tuberculosis infection and bcg vaccination by modulating the interleukin-17 response. | we have previously demonstrated that b cells can shape the immune response to mycobacterium tuberculosis, including the level of neutrophil infiltration and granulomatous inflammation at the site of infection. the present study examined the mechanisms by which b cells regulate the host neutrophilic response upon exposure to mycobacteria and how neutrophilia may influence vaccine efficacy. to address these questions, a murine aerosol infection tuberculosis (tb) model and an intradermal (id) ear b ... | 2013 | 23853593 |
| ethnic variation in inflammatory profile in tuberculosis. | distinct phylogenetic lineages of mycobacterium tuberculosis (mtb) cause disease in patients of particular genetic ancestry, and elicit different patterns of cytokine and chemokine secretion when cultured with human macrophages in vitro. circulating and antigen-stimulated concentrations of these inflammatory mediators might therefore be expected to vary significantly between tuberculosis patients of different ethnic origin. studies to characterise such variation, and to determine whether it rela ... | 2013 | 23853590 |
| critical and independent role for socs3 in either myeloid or t cells in resistance to mycobacterium tuberculosis. | suppressor of cytokine signalling 3 (socs3) negatively regulates stat3 activation in response to several cytokines such as those in the gp130-containing il-6 receptor family. thus, socs3 may play a major role in immune responses to pathogens. in the present study, the role of socs3 in m. tuberculosis infection was examined. all socs3(fl/fl) lysm cre, socs3(fl/fl) lck cre (with socs3-deficient myeloid and lymphoid cells, respectively) and gp130(f/f) mice, with a mutation in gp130 that impedes bin ... | 2013 | 23853585 |
| dna methylation impacts gene expression and ensures hypoxic survival of mycobacterium tuberculosis. | dna methylation regulates gene expression in many organisms. in eukaryotes, dna methylation is associated with gene repression, while it exerts both activating and repressive effects in the proteobacteria through largely locus-specific mechanisms. here, we identify a critical dna methyltransferase in m. tuberculosis, which we term mama. mama creates n⁶-methyladenine in a six base pair recognition sequence present in approximately 2,000 copies on each strand of the genome. loss of mama reduces th ... | 2013 | 23853579 |
| [advances in genome-wide association study of tuberculosis]. | tuberculosis, caused by mycobacterium tuberculosis (mtb), is one of the oldest and most influential diseases in the history due to its devastating effect on health and high mortality rate worldwide. tuberculosis causes more human deaths than any other single infectious disease and the incidence of the tuberculosis is increasing dramatically in recent years. genome-wide association study (gwas) has been used to delineate the genetic basis of tuberculosis, and several susceptibility genes and loci ... | 2013 | 23853352 |
| evaluation of in-house loop-mediated isothermal amplification (lamp) assay for rapid diagnosis of m. tuberculosis in pulmonary specimens. | loop-mediated isothermal amplification (lamp) assay has come forward as a rapid, cost-effective molecular technique for diagnosis of tuberculosis (tb) in developing countries. this study evaluated mycobacterium tuberculosis-specific in-house lamp assay targeting 16s rrna and compared it with other conventional tests and nucleic acid amplification assay (is6110 pcr). | 2013 | 23852783 |
| diagnostic implications of inconsistent results obtained with the xpert mtb/rif assay in detection of mycobacterium tuberculosis isolates with an rpob mutation associated with low-level rifampin resistance. | xpert-mtb/rif is one of the most frequently used molecular screening tests for multidrug-resistant tuberculosis worldwide. we report false-negative assay results in the presence of rpob leu533pro, which is associated with low-level phenotypic rifampin resistance. accurate and timely confirmation of rifampin susceptibility results obtained with xpert-mtb/rif is imperative. | 2013 | 23850949 |
| genotyping and clinical characteristics of multidrug and extensively drug-resistant tuberculosis in a tertiary care tuberculosis hospital in china. | there is a lack of information on the clinical characteristics of multidrug-resistant (mdr) tuberculosis (tb) and extensively drug-resistant (xdr) tb in the jiangxi province of china; furthermore, data have not been reported on the utility of mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (miru-vntr) analyses in genotyping mycobacterium tuberculosis strains isolated from this region. the aim of this study was to analyse the clinical features of patients with mdr and xdr ... | 2013 | 23849244 |
| pathways of il-1β secretion by macrophages infected with clinical mycobacterium tuberculosis strains. | the pro-inflammatory cytokine il-1β is a key mediator of inflammation and plays an important role in the host resistance to mycobacterium tuberculosis infections. to date, most studies have examined the mechanisms of il-1β secretion using laboratory strains of m. tuberculosis and the findings may not be widely applicable to contemporary clinical strains. here, we investigated the primary pathways of il-1β secretion in macrophages infected with a panel of 17 clinical m. tuberculosis isolates, rep ... | 2013 | 23849220 |
| the use of mycobacterial interspersed repetitive unit typing and whole genome sequencing to inform tuberculosis prevention and control activities. | molecular strain typing of mycobacterium tuberculosis has been possible for only about 20 years; it has significantly improved our understanding of the evolution and epidemiology of mycobacterium tuberculosis and tuberculosis disease. mycobacterial interspersed repetitive unit typing, based on 24 variable number tandem repeat unit loci, is highly discriminatory, relatively easy to perform and interpret and is currently the most widely used molecular typing system for tuberculosis surveillance. n ... | 2013 | 23849021 |
| esx-1-induced apoptosis is involved in cell-to-cell spread of mycobacterium tuberculosis. | apoptosis modulation is a procedure amply utilized by intracellular pathogens to favour the outcome of the infection. nevertheless, the role of apoptosis during infection with mycobacterium tuberculosis, the causative agent of human tuberculosis, is subject of an intense debate and still remains unclear. in this work, we describe that apoptosis induction in host cells is clearly restricted to virulent m. tuberculosis strains, and is associated with the capacity of the mycobacteria to secrete the ... | 2013 | 23848406 |
| female sex and discontinuation of isoniazid due to adverse effects during the treatment of latent tuberculosis. | to determine the rate of and risk factors for discontinuation of isoniazid due to adverse effects during the treatment of latent tuberculosis infection in a large, multi-site study. | 2013 | 23845828 |
| limited t cell receptor beta variable repertoire responses to esat-6 and cfp-10 in subjects infected with mycobacterium tuberculosis. | mycobacterium tuberculosis (mtb)-specific antigens, esat-6 or cfp-10 play a key role in diagnosis and control mtb infection. t cell receptor (tcr) reflects the status and function of t cells. however, the features of the tcr beta variable (tcrbv) repertoire used against esat-6 and cfp-10 from mtb subjects have not been well described. the molecular profiles of tcrbv complementarity-determining region 3 (cdr3) in pbmcs with or without esat-6 or cfp-10 stimulation were assayed using a gene melting ... | 2013 | 23845455 |
| synthesis of a mycobacterium tuberculosis tetra-acylated sulfolipid analogue and characterization of the chiral acyl chains using anisotropic nad 2d-nmr spectroscopy. | tetra-o-acylated sulfolipids are metabolites found in the cell wall of mycobacterium tuberculosis, the causative agent of tuberculosis. their role in pathogenesis remains, however, undefined. here we describe a novel access to model tetra-o-acylated trehalose sulfate derivatives having simple acyl chains. the trehalose core was regioselectively protected using a tandem procedure with catalytic iron(iii) chloride hexahydrate and further desymmetrized. model chiral fatty acids, prepared by a zinc- ... | 2013 | 23845048 |
| predicting and analyzing interactions between mycobacterium tuberculosis and its human host. | the outcome of infection by mycobacterium tuberculosis (mtb) depends greatly on how the host responds to the bacteria and how the bacteria manipulates the host, which is facilitated by protein-protein interactions. thus, to understand this process, there is a need for elucidating protein interactions between human and mtb, which may enable us to characterize specific molecular mechanisms allowing the bacteria to persist and survive under different environmental conditions. in this work, we used ... | 2013 | 23844013 |
| identification of residues involved in substrate specificity and cytotoxicity of two closely related cutinases from mycobacterium tuberculosis. | the enzymes belonging to the cutinase family are serine enzymes active on a large panel of substrates such as cutin, triacylglycerols, and phospholipids. in the m. tuberculosis h37rv genome, seven genes coding for cutinase-like proteins have been identified with strong immunogenic properties suggesting a potential role as vaccine candidates. two of these enzymes which are secreted and highly homologous, possess distinct substrates specificities. cfp21 is a lipase and cut4 is a phospholipase a2, ... | 2013 | 23843969 |
| pericardial tuberculosis. | a 53-year-old malnourished man, presented to the emergency room with 2 months of poor appetite, malaise, high spiking fevers, 10 kg weight loss and night sweats; he also noted progressive exertional dyspnea and anterior chest pain developing over the last 2 days. on physical examination he was afebrile, had 90/60 arterial pressure, 100 × min heart rate, pulsus paradoxus, jugular vein engorgement, sudden inspiratory splitting of the second heart sound, and soft heart sounds on auscultation. a con ... | 2013 | 23843490 |
| a convergent and stereoselective synthesis of the glycolipid components phthioceranic acid and hydroxyphthioceranic acid. | | 2013 | 23843294 |
| tpl-2-erk1/2 signaling promotes host resistance against intracellular bacterial infection by negative regulation of type i ifn production. | tuberculosis, caused by mycobacterium tuberculosis, remains a leading cause of mortality and morbidity worldwide, causing ≈ 1.4 million deaths per year. key immune components for host protection during tuberculosis include the cytokines il-12, il-1, and tnf-α, as well as ifn-γ and cd4(+) th1 cells. however, immune factors determining whether individuals control infection or progress to active tuberculosis are incompletely understood. excess amounts of type i ifn have been linked to exacerbated d ... | 2013 | 23842752 |
| progress in targeting cell envelope biogenesis in mycobacterium tuberculosis. | most of the newly discovered compounds showing promise for the treatment of tb, notably multidrug-resistant tb, inhibit aspects of mycobacterium tuberculosis cell envelope metabolism. this review reflects on the evolution of the knowledge that many of the front-line and emerging products inhibit aspects of cell envelope metabolism and in the process are bactericidal not only against actively replicating m. tuberculosis, but contrary to earlier impressions, are effective against latent forms of t ... | 2013 | 23841633 |
| successful treatment in a child with anaplastic large cell lymphoma and coexistence of pulmonary tuberculosis. | a 13-year-old girl was admitted to our department with a history of severe pain of her left axilla and fever. on physical examination, a block of lymph nodes in her left axilla, diffuse papular rash, and red-violet swelling of her supraclavicular and subclavian region were noted. imaging investigations revealed left axillar and supraclavicular lymphadenopathy and a small nodular shade in the upper lobe of her left lung. a biopsy from an axillary lymph node established the diagnosis of anaplastic ... | 2013 | 23841007 |
| mycobacterium tuberculosis strains are differentially recognized by tlrs with an impact on the immune response. | tuberculosis associates with a wide spectrum of disease outcomes. the beijing (bj) lineage of mycobacterium tuberculosis (mtb) is suggested to be more virulent than other mtb lineages and prone to elicit non-protective immune responses. however, highly heterogeneous immune responses were reported upon infection of innate immune cells with bj strains or stimulation with their glycolipids. using both in vitro and in vivo mouse models of infection, we here report that the molecular mechanism for th ... | 2013 | 23840651 |
| phenotypically occult multidrug-resistant mycobacterium tuberculosis: dilemmas in diagnosis and treatment. | the clinical significance of the emergence of mycobacterium tuberculosis (mtb) isolates that contain rpob mutations (genotypic resistance), but are phenotypically susceptible to rifampicin (rif g(r) p(s)), remains uncertain. the aim of this study was to determine the prevalence of mtb cases that demonstrate this discordant rifampicin resistance pattern and to establish whether these patients have poorer treatment outcomes with rifampicin-based regimens. | 2013 | 23838950 |
| multiple samples improve the sensitivity for detection of mixed mycobacterium infections. | by using vntr genotyping, mixed infections of mycobacterium tuberculosis were detected in 11.2% of cases in a prospective study in heilongjiang china, a setting with a high prevalence (87.5%) of beijing family strains. if only one sputum sample had been collected, the study would have underestimated the fraction of mixed infections by 50%. | 2013 | 23838118 |
| first case of mycobacterium tuberculosis transmission by heart transplantation from donor to recipient. | we report the first documented case of a mycobacterium tuberculosis transmission by an orthotopic heart transplantation from the donor to the recipient. mycobacterium tuberculosis positive blood culture showed systemic prevalence of the mycobacteria, however, prophylactic therapy was able to prevent a clinical manifestation of tuberculosis in the recipient. | 2013 | 23838045 |
| [impacts of mycobacterium tuberculosis with different virulence on expressions of ferroportin and ferritin in infected macrophages]. | to explore the time-dependent changes in the expressions of ferritin (fn) and ferroportin (fpn) in macrophages infected with mycobacterium (m.) tuberculosis of highly virulent strain (h37rv strain) and bacillus calmette-guerin (bcg) strain. | 2013 | 23837981 |
| tuberculosis: current state of knowledge: an epilogue. | mycobacterium tuberculosis, the causative agent of tuberculosis (tb), has developed various mechanisms to survive and cause disease in the human host. incomplete understanding of the complex microbe-host interactions has hindered the identification of suitable biomarkers to expedite the development of diagnostic tools, drugs and vaccines. the field effectiveness of directly observed therapy-short course has been compromised by the intrinsic limitations of sputum microscopy and suboptimal adheren ... | 2013 | 23837600 |
| synthesis and structure-activity relationship of 8-substituted protoberberine derivatives as a novel class of antitubercular agents. | the emergence of multi-drug resistant tuberculosis (mdr-tb) has heightened the need for new chemical classes and innovative strategies to tackle tb infections. it is urgent to discover new classes of molecules without cross-resistance with currently used antimycobacterial drugs. | 2013 | 23837573 |
| redesign of substrate specificity and identification of the aminoglycoside binding residues of eis from mycobacterium tuberculosis. | the upsurge in drug-resistant tuberculosis (tb) is an emerging global problem. the increased expression of the enhanced intracellular survival (eis) protein is responsible for the clinical resistance to aminoglycoside (ag) antibiotics of mycobacterium tuberculosis . eis from m. tuberculosis (eis_mtb) and m. smegmatis (eis_msm) function as acetyltransferases capable of acetylating multiple amines of many ags; however, these eis homologues differ in ag substrate preference and in the number of ace ... | 2013 | 23837529 |
| shrinking the fade proteome of mycobacterium tuberculosis: insights into cholesterol metabolism through identification of an α2β2 heterotetrameric acyl coenzyme a dehydrogenase family. | the ability of the pathogen mycobacterium tuberculosis to metabolize steroids like cholesterol and the roles that these compounds play in the virulence and pathogenesis of this organism are increasingly evident. here, we demonstrate through experiments and bioinformatic analysis the existence of an architecturally distinct subfamily of acyl coenzyme a (acyl-coa) dehydrogenase (acad) enzymes that are α2β2 heterotetramers with two active sites. these enzymes are encoded by two adjacent acad (fade) ... | 2013 | 23836861 |
| discovery of an acyclic nucleoside phosphonate that inhibits mycobacterium tuberculosis thyx based on the binding mode of a 5-alkynyl substrate analogue. | the urgent need for new antibiotics poses a challenge to target un(der)exploited vital cellular processes. thymidylate biosynthesis is one such process due to its crucial role in dna replication and repair. thymidylate synthases (ts) catalyze a crucial step in the biosynthesis of thymidine 5-triphosphate (ttp), an elementary building block required for dna synthesis and repair. to date, ts inhibitors have only been successfully applied in anticancer therapy due to their lack of specificity for a ... | 2013 | 23836539 |
| impact of fluoroquinolone resistance on bactericidal and sterilizing activity of a moxifloxacin-containing regimen in murine tuberculosis. | it has been shown previously that fluoroquinolone resistance (defined by resistance to at least 2 mg/liter ofloxacin) has a different impact on moxifloxacin monotherapy depending on the mutation in the sole fluoroquinolone target in mycobacterium tuberculosis, i.e., dna gyrase. since tuberculosis treatment relies on multidrug therapy, we wished to determine the impact of fluoroquinolone resistance on the bactericidal and sterilizing activity of a second-line antituberculous regimen containing mo ... | 2013 | 23836169 |
| overexpression of the rv0805 phosphodiesterase elicits a camp-independent transcriptional response. | the rv0805 gene in mycobacterium tuberculosis encodes a metallophosphoesterase which shows camp-hydrolytic activity. overexpression of rv0805 has been used as a tool to lower intracellular camp levels and thereby elucidate the roles of camp in mycobacteria. here we show that levels of camp in m. tuberculosis were lowered by only ∼30% following overexpression of rv0805, and transcript levels of a number of genes, which include those associated with virulence and the methyl citrate cycle, were alt ... | 2013 | 23835087 |
| the proportions of people living with hiv in low and middle-income countries who test tuberculin skin test positive using either a 5 mm or a 10 mm cut-off: a systematic review. | a positive tuberculin skin test (tst) is often defined by skin induration of ≥10 mm in people who are hiv-seronegative. however, to increase sensitivity for detection of mycobacterium tuberculosis infection in the context of impaired immune function, a revised cut-off of ≥5 mm is used for people living with hiv infection. the incremental proportion of patients who are included by this revised definition and the association between this proportion and cd4+ cell count are unknown. | 2013 | 23834892 |
| use of the xpert(®) mtb/rif assay for diagnosing pulmonary tuberculosis comorbidity and multidrug-resistant tb in obstetrics and gynaecology inpatient wards at the university teaching hospital, lusaka, zambia. | in high-tuberculosis (tb)-endemic countries, comorbidity of pulmonary tb in hospitalised patients with non-communicable diseases is well documented. in this study, we evaluated the use of the xpert(®) mtb/rif assay for the detection of concomitant pulmonary tb in patients admitted to the university teaching hospital, lusaka, zambia, with a primary obstetric or gynaecological condition. | 2013 | 23834035 |
| selecting criteria for improving results of an immunochromatographic assay for mycobacterium tuberculosis complex identification from bact/alert cultures. | we describe a combined macro and microscopic criteria for rapid presumptive differentiation between mycobacterium tuberculosis complex (mtc) and nontuberculous mycobacteria (ntm) evaluated by two independent observers. this strategy achieved rapid mtc identification in most cases (95.8% expert observer and 91.6% novice observer) with significant savings compared to more expensive and unnecessary tests. | 2013 | 23830849 |
| application of flip method for differentiation of mycobacterium tuberculosis strains in comparison to commonly used methods, spoligotyping and miru-vntr typing. | the current "gold standard" in molecular epidemiological studies of mycobacterium tuberculosis is is6110 rflp based on is6110 polymorphism. however pcr-based methods are becoming increasingly important. recently, fast ligation-mediated pcr (flip), based on is6110 polymorphism was proposed. in this study, the discriminatory power of flip, spoligotyping and miru-vntr typing, in differentiation of m. tuberculosis isolates was compared. the discriminatory index (hgi) of spoligotyping, miru-vntr anal ... | 2013 | 23829080 |
| dna microarray gene expression profile of mycobacterium tuberculosis when exposed to osthole. | tuberculosis (tb), affecting one-third of the global population, kills an estimated two to three million people every year. the development of drug resistance is becoming a serious threat to any attempt to control this disease, which underscores the need for new agents targeting mycobacterium tuberculosis (m. tuberculosis). osthole (7-methoxy-8-isopentenoxycoumarin) is a coumarin derivative present in many medicinal plants. previous studies have shown that osthole possesses antimycobacterial eff ... | 2013 | 23829074 |
| xpert® mtb/rif in pleural fluid for the diagnosis of tuberculosis. | an automated nucleic acid amplification assay that simultaneously identifies mycobacterium tuberculosis and rifampicin resistance, the xpert® mtb/rif test, has undergone extensive evaluation in sputum samples. our aim was to define its diagnostic accuracy when performed on pleural fluid specimens. in 67 patients with pleural effusions, of whom half had tuberculous pleuritis, xpert yielded 15% sensitivity and 100% specificity in the detection of tuberculosis (tb). positive xpert results tended to ... | 2013 | 23827859 |
| mycobacterium tuberculosis ppe protein rv0256c induces strong b cell response in tuberculosis patients. | tuberculosis (tb) is one of the most important diseases of humans and major public health problem worldwide. early and accurate diagnosis of tb is necessary for the treatment, prevention, and control of tb. therefore, it is important to identify suitable antigens that can differentiate active tuberculosis patients from bcg-vaccinated individuals. in the present study, we have used rv0256c (ppe2) protein of mycobacterium tuberculosis to screen the sera of infected patients belonging to different ... | 2014 | 23827809 |
| luciferase reporter phage phae85 for rapid detection of rifampicin resistance in clinical isolates of mycobacterium tuberculosis. | to evaluate luciferase reporter phage (lrp) phae85 in rapid detection of rifampicin resistance in a region where tb is endemic. | 2013 | 23827152 |
| is routine bronchoscopic culture indicated in areas with low tuberculosis prevalence? | tertiary care university-affiliated medical centre. | 2013 | 23827036 |
| molecular and phenotypic characterisation of mycobacterium tuberculosis resistant to anti-tuberculosis drugs. | dr cetrángolo hospital, buenos aires, argentina. | 2013 | 23827034 |
| virulence of clinical mycobacterium tuberculosis strains in lodz, poland. | the transmission of tuberculosis (tb) in the population and the development of disease are determined not only by the patient's immunological status, but also by the virulence of mycobacterium tuberculosis strains. | 2013 | 23827033 |
| strain diversity of mycobacteria isolated from pulmonary tuberculosis patients at debre birhan hospital, ethiopia. | ethiopia ranks seventh in the list of 22 high tuberculosis (tb) burden countries, with an incidence rate of 379 cases per 100,000 population for tb all forms. however, information on the genomic diversity of mycobacterium tuberculosis in ethiopia is limited. | 2013 | 23827032 |
| analysis of differentially expressed proteins in late-stationary growth phase of mycobacterium tuberculosis h37rv. | mycobacterium tuberculosis is a causative agent of tuberculosis (tb). the ability of m. tuberculosis to be quiescent in the cell has caused the emergence of latent infection. a comprehensive proteomic analysis of m. tuberculosis h37rv over three growth phases, namely mid-log (14-day culture), early stationary (28-day culture), and late stationary (50-day culture), was performed in order to study the change in proteome from the mid-log phase to late-stationary phase. combination methods of two-di ... | 2014 | 23826872 |
| recombinant esat-6-cfp10 fusion protein induction of th1/th2 cytokines and foxp3 expressing treg cells in pulmonary tb. | early secretory antigenic target 6 (esat-6) and culture filtrate protein 10 (cfp-10) are mycobacterium tuberculosis (mtb)-specific antigens that are secreted by actively metabolising bacteria and contribute to the virulence of the bacteria. their ability to induce treg and th2 responses, particularly during the first two weeks of treatment, has not been comprehensively examined to date. the purpose of this work was to characterise th1, th2 and treg responses to resat-6-cfp10 fusion protein in tb ... | 2013 | 23826366 |
| genetic diversity of mycobacterium tuberculosis in peru and exploration of phylogenetic associations with drug resistance. | there is limited available data on the strain diversity of m tuberculosis in peru, though there may be interesting lessons to learn from a setting where multidrug resistant tb has emerged as a major problem despite an apparently well-functioning dots control programme. | 2013 | 23826083 |
| comparison of four dna extraction methods for detecting mycobacterium tuberculosis by real-time pcr and its clinical application in pulmonary tuberculosis. | china is one of the countries with a high burden of mycobacterium tuberculosis (mtb) infection. one challenge for the earlier diagnosis of tuberculosis is the dna extraction of mtb. this study was to compare four mtb dna extraction methods, and use the best one in the diagnosis of pulmonary tuberculosis. | 2013 | 23825755 |
| autophagic killing effects against mycobacterium tuberculosis by alveolar macrophages from young and aged rhesus macaques. | non-human primates, notably rhesus macaques (macaca mulatta, rm), provide a robust experimental model to investigate the immune response to and effective control of mycobacterium tuberculosis infections. changes in the function of immune cells and immunosenescence may contribute to the increased susceptibility of the elderly to tuberculosis. the goal of this study was to examine the impact of age on m. tuberculosis host-pathogen interactions following infection of primary alveolar macrophages de ... | 2013 | 23825603 |
| modification of clearview tuberculosis (tb) enzyme-linked immunosorbent assay for tb patients not infected with hiv. | diagnosis of active tuberculosis by detection of urinary lipoarabinomannan (ulam) from mycobacterium tuberculosis is an attractive approach. concentrating urine 100-fold allowed quantitation of ulam at levels equal to picograms/ml of nonconcentrated urine. the approach of concentrating urine 100-fold improved the clinical sensitivity of the clearview tb enzyme-linked immunosorbent assay (elisa) from 7% to 57% yet impaired its specificity from 97% to 89%. (this study has been registered at univer ... | 2013 | 23825194 |
| transcriptome analysis reveals novel entry mechanisms and a central role of src in host defense during high multiplicity mycobacterial infection. | mycobacterium tuberculosis (mtb) infects an estimated one-third of the global population and is one of the main causes of mortality from an infectious agent. the characteristics of macrophages challenged by mtb with a high multiplicity of infection (moi), which mimics both clinical disseminated infection and granuloma formation, are distinct from macrophages challenged with a low moi. to better understand the cross talk between macrophage host cells and mycobacteria, we compared the transcriptio ... | 2013 | 23824656 |
| infliximab does not lead to reduction in the interferon-gamma and lymphoproliferative responses of patients with moderate to severe psoriasis. | treatment of patients with immune-mediated inflammatory diseases with anti-tumour necrosis factor (anti-tnf) agents increases the risk of tuberculosis reactivation, suggesting that it may affect their cellular immune responses. we evaluated cellular immune responses of 12 severe psoriasis patients before and during infliximab treatment. peripheral blood mononuclear cells were stimulated with phytohaemagglutinin, the superantigen enterotoxin b (seb), a cytomegalovirus lysate (cmv), and mycobacter ... | 2014 | 23824243 |
| the mycobacterium tuberculosis regulatory network and hypoxia. | we have taken the first steps towards a complete reconstruction of the mycobacterium tuberculosis regulatory network based on chip-seq and combined this reconstruction with system-wide profiling of messenger rnas, proteins, metabolites and lipids during hypoxia and re-aeration. adaptations to hypoxia are thought to have a prominent role in m. tuberculosis pathogenesis. using chip-seq combined with expression data from the induction of the same factors, we have reconstructed a draft regulatory ne ... | 2013 | 23823726 |
| simple, direct drug susceptibility testing technique for diagnosis of drug-resistant tuberculosis in resource-poor settings. | the korean institute of tuberculosis, seoul, republic of korea. | 2013 | 23823178 |
| synthesis and bioevaluation of some new isoniazid derivatives. | the aim of the study was to synthesize some new compounds with potential anti-tuberculosis activity, containing isoniazid and α,β-unsaturated thiocinnamamide-like thioamides as precursors. the obtained derivatives were evaluated regarding their biological activity (antioxidant and antibacterial), as well as their influence on the eukaryotic cell cycle. the results suggested that the newly obtained derivatives of isoniazid exhibited different biological activities, depending on their structure; t ... | 2013 | 23823011 |
| assessment of clofazimine activity in a second-line regimen for tuberculosis in mice. | although observational studies suggest that clofazimine-containing regimens are highly active against drug-resistant tuberculosis, the contribution of clofazimine for the treatment of this disease has never been systematically evaluated. | 2013 | 23822735 |
| thiosemicarbazones and their antimycobacterial effects. | antimycobacterial effects of thiosemicarbazones were discovered in the late 1940s. the best known representative of these compounds is thioacetazone that has been used in the therapy of tuberculosis since the turn of the 1940s and 1950s. at present, it is used only rarely since it exhibits severe side effects. this paper deals with the antimycobacterial effects of thiosemicarbazones and n,n-dimethylthiosemicarbazones derived from 5-alkyl-2-acetylpyrazines. some of these compounds displayed high ... | 2013 | 23822572 |
| dxr inhibition by potent mono- and disubstituted fosmidomycin analogues. | the antimalarial compound fosmidomycin targets dxr, the enzyme that catalyzes the first committed step in the mep pathway, producing the essential isoprenoid precursors, isopentenyl diphosphate and dimethylallyl diphosphate. the mep pathway is used by a number of pathogens, including mycobacterium tuberculosis and apicomplexan parasites, and differs from the classical mevalonate pathway that is essential in humans. using a structure-based approach, we designed a number of analogues of fosmidomyc ... | 2013 | 23819803 |
| [estimating the prevalence of tuberculosis infection among healthcare workers in our hospital by repeat qft-g testing]. | the quantiferon-tb (qft) blood test is the major tool for the diagnosis of mycobacterium tuberculosis (tb) infection among healthcare workers (hcws). we used qft tests to estimate the prevalence of tb infection among hcws in our hospital. | 2013 | 23819316 |
| [evaluation of jata(12)-variable number of tandem repeats as a marker of the source of tuberculosis outbreaks in osaka]. | to evaluate the usefulness of the jata (12)-variable number of tandem repeats (vntr) system for identifying the source of mycobacterium tuberculosis outbreaks. design; jata(12)-vntr genotyping was performed on m. tuberculosis isolates from a total of 206 patients in whom group infection was confirmed by epidemiological studies ("group infection"), as well as from 64 patient clusters in whom group infection was suspected but not confirmed ("non-group infection"). the patients were diagnosed in os ... | 2013 | 23819315 |
| [clustering analysis of mycobacterium tuberculosis using the jata(12)-vntr system for molecular epidemiological surveillance in broad areas of japan]. | japan anti-tuberculosis association (jata) (12)-variable numbers of tandem repeats (vntr) is a standard method for genotyping of clinical isolates of mycobacterium tuberculosis in japan. as a model study for nationwide surveillance, this study aimed to describe the tendency and frequency of genotypes of m. tuberculosis in a large number of clinical samples. | 2013 | 23819314 |
| mycobacterium tuberculosis transcriptome profiling in mice with genetically different susceptibility to tuberculosis. | whole transcriptome profiling is now almost routinely used in various fields of biology, including microbiology. in vivo transcriptome studies usually provide relevant information about the biological processes in the organism and thus are indispensable for the formulation of hypotheses, testing, and correcting. in this study, we describe the results of genome-wide transcriptional profiling of the major human bacterial pathogen m. tuberculosis during its persistence in lungs. two mouse strains d ... | 2013 | 23819037 |
| crystal structure of the n-terminal domain of ecca₁ atpase from the esx-1 secretion system of mycobacterium tuberculosis. | ecca1 is an important component of the type vii secretion system (t7ss) that is responsible for transport of virulence factors in pathogenic mycobacteria. ecca1 has an n-terminal domain of unknown function and a c-terminal aaa+ (atpases associated with various cellular activities) domain. here we report the crystal structure of the n-terminal domain of ecca1 from mycobacterium tuberculosis, which shows an arrangement of six tetratricopeptide repeats that may mediate interactions of ecca1 with se ... | 2014 | 23818233 |
| identification of 1-chloro-2-formyl indenes and tetralenes as novel antistaphylococcal agents exhibiting sortase a inhibition. | tetralene and indene compounds have shown inhibitory activity against human pathogen, mycobacterium tuberculosis. their potential use as antistaphylococcal agent against drug-resistant staphylococcus aureus has not been explored so far. we determined in vitro antistaphylococcal activity and mechanism of action of these compounds as sortase a inhibitors through in silico analysis followed by biological assays. tetralene and indene series were tested against s. aureus strains mtcc96, mrsa, and va3 ... | 2014 | 23817664 |
| cutting edge: direct recognition of infected cells by cd4 t cells is required for control of intracellular mycobacterium tuberculosis in vivo. | effector t cells control intracellular infection by secreting cytokines and through contact-dependent cytolysis. because cytokines can diffuse and act at a distance, we determined whether cytokine diffusion is sufficient to control mycobacterium tuberculosis or whether direct recognition of infected cells by cd4 t cells is required. using mhc class ii (mhc ii) mixed bone marrow chimeras, we compared the bacterial burdens in lung myeloid cells that were capable (mhc ii(+/+)) or not (mhc ii(-/-)) ... | 2013 | 23817429 |
| antimycobacterial activity of citrullus colocynthis (l.) schrad. against drug sensitive and drug resistant mycobacterium tuberculosis and mott clinical isolates. | citrullus colocynthis (cucurbitaceae), a folk herbal medicine and traditionally used natural remedy for tuberculosis in india has been studied to validate its antitubercular activity against drug sensitive and drug resistant (including multidrug resistant) mycobacterium tuberculosis and mycobacterium other than tuberculosis (mott) bacilli. | 2013 | 23816500 |
| encephalitis due to mycobacterium tuberculosis in france. | two hundred and fifty-three patients were included in a study on the etiology of encephalitis, carried out in france in 2007. tuberculosis was the second most frequently identified cause, after hsv and with the same number of cases as vzv. the authors report the specific features of patients presenting with tuberculosis encephalitis (te). | 2013 | 23816146 |
| isothermal detection of multiple point mutations by a surface plasmon resonance biosensor with au nanoparticles enhanced surface-anchored rolling circle amplification. | in this study, we developed a surface plasmon resonance (spr) dna biosensor method using surface-anchored rolling circle amplification (rca) and au nanoparticles modified probes (aunps) to isothermally detect multiple point mutations associated with drug-resistance in multidrug-resistant mycobacterium tuberculosis (mdrtb). a set of probes contains an allele-specific padlock probe (plp), a capture probe and an aunps. the linear plps, circularized by ligation upon the recognition of the point muta ... | 2013 | 23811476 |
| the double burden. | one third of the world's population is latently infected with mycobacterium tuberculosis, and with the lifestyle changes succeeding the on-going urbanization, populations already burdened by tuberculosis are experiencing a dramatic increase in chronic diseases, with diabetes being a serious challenge. tuberculosis and diabetes are not only becoming co-existing diseases. in fact, the diseases interact, and there is evidence to suggest that especially diabetes disease increases the susceptibility ... | 2013 | 23809978 |
| 3d-qsar comfa studies on sulfonamide inhibitors of the rv3588c β-carbonic anhydrase from mycobacterium tuberculosis and design of not yet synthesized new molecules. | the human pathogen mycobacterium tuberculosis contains three β-carbonic anhydrases (cas, ec 4.2.1.1) in its genome. inhibition of some of these cas was shown to modulate the growth of m. tuberculosis. 3d-qsar comparative molecular field analyses (comfa) were carried out on inhibitors of the enzyme rv3588c (also denominated mtca 2). a series of sulfonamides known to inhibit mtca 2, including some diazenylbenzenesulfonamides, was considered in our study. the predictive ability of the model was ass ... | 2014 | 23808803 |
| [comparing performance of "tb-biochip", "xpert mtb/rif" and "genotype mtbdrplus" assays for fast identification of mutations in the mycobacterium tuberculosis complex in sputum from tb patients]. | the frequency of mutations causing drug resistance in mtb isolates were studied in the respiratory material obtained from tb-patients in the moscow region. in izoniazid-resistant isolates, the most prevalent mutation was found to be the ser315thr substitution in the katg gene (15.8%) whereas the most frequent mutations in multidrug-resistant isolates were ser531leu and ser315thr in the rpob and katg genes (26.3%), or a combination of these two substitutions with a t15 mutation in the inha gene ( ... | 2013 | 23808160 |
| high prevalence of tuberculosis diagnosed during autopsy examination at muhimbili national hospital in dar es salaam, tanzania. | the primary aims of tuberculosis (tb) control programmes is early diagnosis and prompt treatment of infectious cases to limit transmission. failure to diagnose and adequately treat tb could lead to premature death and unrecognized transmission of mycobacterium tuberculosis. the proportion of missed tb cases has not been reported in tanzania. the objective of this study was to quantify the number of cases of tb identified by autopsy. deceased morbid bodies from muhimbili national hospital were in ... | 2013 | 26591706 |
| multianalyte microphysiometry of macrophage responses to phorbol myristate acetate, lipopolysaccharide, and lipoarabinomannan. | this study examined the hypothesis that mycobacterial antigens generate different metabolic responses in macrophages as compared to gram-negative effectors and macrophage activators. the metabolic activation of macrophages by pma is a useful tool for studying virulent agents and can be compared to other effectors. while phorbol myristate acetate (pma) is commonly used to study macrophage activation, the concentration used to create this physiological response varies. the response of raw-264.7 ma ... | 2013 | 25798034 |
| assessment of detection efficacy of mycobacterium tuberculosis in sputum samples by real time pcr based method. | tuberculosis (tb) is a major public health problem in nepal and ranks as one of the most prevalent communicable diseases throughout the country. in nepal, 45% of total population is infected with tb and 40,000 people get tb every year. twenty thousand new sputum positive cases are seen every year and 5000-7000 people die each year from tb. thirty sputum samples were collected from sukraraj tropical and infectious disease hospital, teku, kathmandu, nepal and the comparative study of acid-fast bac ... | 2013 | 25328545 |
| bedaquiline for the treatment of pulmonary, multidrug-resistant tuberculosis in adults. | after aids, tuberculosis (tb) is the leading killer worldwide due to a single infectious agent. recently, drug-resistant strains of mycobacterium tuberculosis elicited even more severe versions of tb. bedaquiline inhibits mycobacterial atp synthase. it shows potent and selective activity in vitro against m. tuberculosis, and in vivo against murine models of tb. bedaquiline can be combined with antituberculosis and antiretroviral agents. the product displays good oral absorption, has a long termi ... | 2013 | 23807939 |
| rapid detection of mycobacterium tuberculosis in clinical samples by multiplex polymerase chain reaction (mpcr). | although the multi-copy and specific element is6110 provides a good target for the detection of mycobacterium tuberculosis complex by pcr techniques, the emergence of is6110-negative strains suggested that false negative may occur if is6110 alone is used as the target for detection. in this report, a multiplex polymerase chain reaction (mpcr) system was developed using primers derived from the insertion sequence is6110 and an is-like elements designated as b9 (genbank accession no. u78639.1) to ... | 2013 | 23807412 |
| fluoroquinolone susceptibility in mycobacterium tuberculosis after pre-diagnosis exposure to older- versus newer-generation fluoroquinolones. | fluoroquinolone exposure before tuberculosis (tb) diagnosis is common. we anticipated that exposure to older-generation fluoroquinolones is associated with greater fluoroquinolone mics in mycobacterium tuberculosis than exposure to newer agents. a nested case-control study was performed among newly diagnosed tb patients reported to the tennessee department of health (january 2002-december 2009). each fluoroquinolone-resistant case (n=25) was matched to two fluoroquinolone-susceptible controls (n ... | 2013 | 23806638 |
| human mycobacterium tuberculosis cd8 t cell antigens/epitopes identified by a proteomic peptide library. | identification of cd8(+) t cell antigens/epitopes expressed by human pathogens with large genomes is especially challenging, yet necessary for vaccine development. immunity to tuberculosis, a leading cause of mortality worldwide, requires cd8(+) t cell immunity, yet the repertoire of cd8 antigens/epitopes remains undefined. we used integrated computational and proteomic approaches to screen 10% of the mycobacterium tuberculosis (mtb) proteome for cd8 mtb antigens. we designed a weighting schema ... | 2013 | 23805289 |