| measurement of 1j(ni,calpha(i)), 1j(ni,c'i-1), 2j(ni,calpha(i-1)), 2j(h(n)i,c'i-1) and 2j(h(n)i,calpha(i)) values in 13c/15n-labeled proteins. | use of partial or selective (13)c/(15)n labeling of specific amino acid residues in a given protein to measure the values of (1)j((15)n(i),(13)c(alpha) (i)), (2)j((1)h(n),(13)c(alpha) (i)), (2)j((15)n(i),(13)c(alpha) (i-1)), (1)j((15)n(i),(13)c'(i-1)) and (2)j((1)h(n),(13)c'(i-1)) is described. this was achieved by recording a sensitivity-enhanced 2d [(15)n-(1)h] hsqc experiment, without mixing the spin states of c(alpha) and c' during the course of entire experiment. | 2005 | 15674817 |
| immunization with a tetramer derivative of an anti-inflammatory pentapeptide produced by entamoeba histolytica protects gerbils (meriones unguiculatus) against experimental amoebic abscess of the liver. | axenically grown entamoeba histolytica produces a pentapeptide (met-gln-cys-asn-ser) with several anti-inflammatory properties, including the inhibition of human monocyte locomotion (monocyte locomotion inhibitory factor (mlif)). a construct displays the same effects as the native material. it remains to be seen if mlif is used, or even produced in vivo by the tissue-invading parasite. if mlif were to be relevant in invasive amoebiasis, immunizing against it could diminish this parasite advantag ... | 2004 | 15679631 |
| survey for intestinal parasites in belize, central america. | a stool survey was carried out in 5 villages in the toledo district of the central american country of belize. eighty-two percent of a total population of 672 participated. the stools were examined by the formalin-ethyl-acetate concentration technique. sixty-six percent of the population was found to have one or more intestinal parasites. the most common infection was hookworm (55%) followed by ascaris lumbricoides (30%), entamoeba coli (21%), trichuris trichiura (19%), giardia lamblia (12%), io ... | 2004 | 15689057 |
| [entamoeba histolytica and entamoeba dispar: differentiation methods and implications]. | entamoeba histolytica and entamoeba dispar are two species morphologically identical (except hematophagous trophozoites) but one of them is pathogenic. sensitive and specific molecular techniques which are able to distinguish e. histolytica from e. dispar have been developed recently. detection of antigen in stool using the elisa method is the diagnostic test method of choice for clinical use in the developing world. it is rapid and simple. cultures for zymodeme analysis and pcr detection of the ... | 2005 | 15689308 |
| isolation and functional analysis of a chk2 homologue from entamoeba histolytica. | mammalian chk2 is a ser/thr kinase required for cell-division arrest induced by dna damage. we found six new kinase genes of entamoeba histolytica by analysis in silico. one of the kinase genes was a homologue of human chk2 gene. the chk2 homologue gene (eh chk2) was expected to encode 398 amino acids and showed nearly 50% homology to human chk2 in amino acid sequence. eh chk2 had a catalytic domain of ser/thr kinase and a fork head-associated (fha) domain that is highly conserved among chk2 hom ... | 2005 | 15710546 |
| acto-myosin cytoskeleton dependent viscosity and shear-thinning behavior of the amoeba cytoplasm. | the mechanical behavior of the human parasite entamoeba histolytica plays a major role in the invasive process of host tissues and vessels. in this study, we set up an intracellular rheological technique derived from magnetic tweezers to measure the viscoelastic properties within living amoebae. the experimental setup combines two magnetic fields at 90 degrees from each other and is adapted to an inverted microscope, which allows monitoring of the rotation of pairs of magnetic phagosomes. we obs ... | 2005 | 15711811 |
| entamoeba histolytica: identification of a distinct beta2 integrin-like molecule with a potential role in cellular adherence. | entamoeba histolytica infection causes dysentery, intestinal colitis, and hepatic abscess in an estimated 50 million people worldwide. attachment of e. histolytica trophozoites to intestinal epithelium and vascular endothelium during liver metastasis results in an inflammatory process. we report the identification of a distinct amebic beta2 integrin (cd18)-like molecule which affords adherence to tnf-alpha-activated endothelial cells. data from flow cytometry and indirect immunofluorescence assa ... | 2005 | 15713444 |
| entamoeba histolytica: inhibition of cellular functions by overexpression of ehgef1, a novel rho/rac guanine nucleotide exchange factor. | the molecular, biochemical, and cellular characterization of ehgef1 protein is described. complete cdna sequence of 1890 bp revealed an open reading frame that encodes a protein of 69 kda. ehgef1 is constituted of dbl homology domain, pleckstrin homology domain, and several putative regulation sites. studies of guanine nucleotide exchange activity of ehgef1 on several gtpases from entamoeba histolytica and homo sapiens showed preferential activation on ehracg, suggesting that ehgef1 protein coul ... | 2005 | 15713446 |
| gastrointestinal parasites of the guenons of western uganda. | from january 1998 to december 2002, we collected 293 fecal samples from free-ranging individuals of the 4 guenon species of western uganda, i.e., redtail guenons (cercopithecus ascanius), blue monkeys (cercopithecus mitis), l'hoesti monkeys (cercopithecus lhoesti), and vervet monkeys (cercopithecus aethiops), to quantify the prevalence of gastrointestinal parasites. helminth eggs, larvae, and protozoan cysts were isolated by sodium nitrate flotation and fecal sedimentation. helminth parasites we ... | 2004 | 15715228 |
| identification, structure, and phylogenetic relationships of a mitogen-activated protein kinase homologue from the parasitic protist entamoeba histolytica. | a gene encoding mitogen-activated protein kinase (mapk) from the human enteric parasite, entamoeba histolytica has been identified. sequence analyses of the polymerase chain reaction (pcr) and reverse transcription pcr (rt-pcr) products reveal that the ehmapk gene is intronless and encodes a protein of 352 amino acids. ehmapk shows significant homology with other mapks and contains the 11 subdomains including the invariant residues characteristic of serine/threonine protein kinases. the mapk sig ... | 2005 | 15716029 |
| [prevalence of intestinal parasitosis in the city of eirunepé, amazon]. | samples of 413 patient were analyzed and positivity was found in 64.4% (266) of the exams. the intestinal parasites most prevalent were: ascaris lumbricoides (35.6%); trichuris trichiura (18.6%); ancylostomides (9.9%); strongyloides stercoralis (1%), enterobius vermiculares (0,5%); entamoeba histolytica (13.3%) and giardia lamblia (1%). the presence of varied degrees of anemia were detected in 39.8% of the exams. | 2005 | 15717101 |
| activation of mapk kinase pathway by gal/galnac adherence lectin of e. histolytica: gateway to host response. | amoebiasis caused by the protozoan parasite entamoeba histolytica is one of the leading parasitic causes of morbidity and mortality in the developing countries. among the variety of virulence factors, an adherence lectin (gal/galnac, 260 kda) has been known to mediate colonization and subsequent host responses. it is a major cell surface antigen which is universally recognized by the immune sera of patients with amoebic liver abscess (ala). the role of this lectin in cytolysis and phagocytosis o ... | 2005 | 15724442 |
| synthesis and antiamoebic activities of 1-n-substituted cyclised pyrazoline analogues of thiosemicarbazones. | a series of 21 new 1-n-substituted cyclised pyrazoline analogues of thiosemicarbazones were synthesised by cyclisation of mannich bases with thiosemicarbazides of variegated nature. the chemical structures of the compounds were proved by uv, ir, (1)h nmr, (13)c nmr spectroscopic data and elemental analyses. the antiamoebic activities of these compounds were evaluated by microdilution method against hm1:1mss strain of entamoeba histolytica. it was found that 3-chloro and 3-bromo substituents on t ... | 2005 | 15727873 |
| antiamoebic and toxicity studies of a carbamic acid derivative and its therapeutic effect in a hamster model of hepatic amoebiasis. | amoebiasis is an important public health problem in developing countries. entamoeba histolytica, the causative agent of amoebiasis, may develop resistance to nitroimidazoles, a group of drugs considered to be the most effective against this parasitic disease. therefore, research on new drugs for the treatment of this common infection still constitutes an important therapeutic demand. in the present study we determined the effects of a carbamate derivative, ethyl 4-chlorophenylcarbamate (c4), on ... | 2005 | 15728919 |
| the genome of the protist parasite entamoeba histolytica. | entamoeba histolytica is an intestinal parasite and the causative agent of amoebiasis, which is a significant source of morbidity and mortality in developing countries. here we present the genome of e. histolytica, which reveals a variety of metabolic adaptations shared with two other amitochondrial protist pathogens: giardia lamblia and trichomonas vaginalis. these adaptations include reduction or elimination of most mitochondrial metabolic pathways and the use of oxidative stress enzymes gener ... | 2005 | 15729342 |
| entamoeba histolytica: focal adhesion kinase and erk phosphorylation are altered in the cytoskeleton mutant bg-3. | the entamoeba histolytica mutant bg-3 has several altered cytoskeletal properties, including the distribution of actin and certain surface characteristics such as osmolarity and electrophoretic mobility. by western blot analysis and assays for cell adhesion to collagen, we demonstrate that mutant bg-3 shows an increase in the phosphorylation levels of protein kinases that participate in proliferation, adhesion and migration, such as focal adhesion kinase and map kinase (erk2), and that it has al ... | 2005 | 15729589 |
| [a dutchman from mali with a perianal ulcer caused by cutaneous amoebiasis]. | a 66-year-old dutchman, living in mali, presented with an extensive progressive perianal ulcer despite local and antibiotic treatment. microscopic examination of the stool revealed entamoeba histolytica/dispar cysts and phagocytosing trophozoites were seen in fresh scrapings of the ulcer, a diagnostic feature of infection with e. histolytica. the diagnosis was cutaneous amoebiasis and the patient was effectively treated with metronidazole and local debridements. primary cutaneous amoebiasis is a ... | 2005 | 15730040 |
| roles of cell adhesion and cytoskeleton activity in entamoeba histolytica pathogenesis: a delicate balance. | the protozoan parasite entamoeba histolytica colonizes the human large bowel. invasion of the intestinal epithelium causes amoebic colitis and opens the route for amoebic liver abscesses. the parasite relies on its dynamic actomyosin cytoskeleton and on surface adhesion molecules for dissemination in the human tissues. here we show that the galactose/n-acetylgalactosamine (gal/galnac) lectin clusters in focal structures localized in the region of e. histolytica that contacts monolayers of entero ... | 2005 | 15731078 |
| invasive amebiasis as an emerging parasitic disease in patients with human immunodeficiency virus type 1 infection in taiwan. | whether risk of invasive amebiasis due to entamoeba histolytica is higher among human immunodeficiency virus (hiv)-infected persons than uninfected persons remains unclear, although intestinal colonization by entamoeba dispar is common among men who have sex with men. our objective was to determine the prevalence of invasive amebiasis and intestinal colonization by e histolytica and e dispar in hiv-infected persons and uninfected controls. | 2005 | 15738369 |
| entamoeba histolytica tata-box binding protein binds to different tata variants in vitro. | the ability of entamoeba histolytica tata binding protein (ehtbp) to interact with different tata boxes in gene promoters may be one of the key factors to perform an efficient transcription in this human parasite. in this paper we used several tata variants to study the in vitro ehtbp dna-binding activity and to determine the tata-ehtbp dissociation constants. the presence of ehtbp in complexes formed by nuclear extracts (ne) and the tatttaaa oligonucleotide, which corresponds to the canonical t ... | 2005 | 15752353 |
| entamoeba histolytica: intracellular distribution of the sec61alpha subunit of the secretory pathway and down-regulation by antisense peptide nucleic acids. | the sec61alpha protein is defined as a highly conserved essential integral component of the endoplasmic reticulum in eukaryotic cells. we report a detailed immunolocalization of the entamoeba histolytica homologue of the sec61alpha subunit (ehsec61alpha), which shows an irregular pattern throughout the cell and is also found on the cell surface, its effective down-regulation by means of antisense peptide nucleic acids and its effects on cell proliferation, subcellular distribution of two virulen ... | 2005 | 15755422 |
| comparative genomic hybridizations of entamoeba strains reveal unique genetic fingerprints that correlate with virulence. | variable phenotypes have been identified for entamoeba species. entamoeba histolytica is invasive and causes colitis and liver abscesses but only in approximately 10% of infected individuals; 90% remain asymptomatically colonized. entamoeba dispar, a closely related species, is avirulent. to determine the extent of genetic diversity among entamoeba isolates and potential genotype-phenotype correlations, we have developed an e. histolytica genomic dna microarray and used it to genotype strains of ... | 2005 | 15755913 |
| [liver abscess due to a native amoeba: a clinical case study and literature review]. | | 2005 | 15757593 |
| identification of ehicp1, a chagasin-like cysteine protease inhibitor of entamoeba histolytica. | based on the entamoeba histolytica genome project (www.sanger.ac.uk/project/e_histolytical/) we have identified a cysteine protease inhibitor, ehicp1 (amoebiasin 1), with significant homology to chagasin. recombinant ehicp1 inhibited the protease activity of papain and that of a trophozoite lysate with ki's in the picomolar range. by immunocytology, we localized the endogenous approximately 13 kda ehicp1 in a finely dotted subcellular distribution discrete from the vesicles containing the amoebi ... | 2005 | 15757643 |
| a lysine- and glutamic acid-rich protein, kerp1, from entamoeba histolytica binds to human enterocytes. | contact-dependent cytolysis of host cells by entamoeba histolytica is an important hallmark of amoebiasis that points out the importance of molecules involved in the interaction between the parasite and the human cells. to decipher the molecular and cellular mechanisms supporting the invasion of the intestinal epithelium by e. histolytica, we analysed proteins involved in the interaction of the parasite with enterocytes. affinity chromatography revealed several amoebic proteins interacting with ... | 2005 | 15760457 |
| a unique rab gtpase, ehraba, is involved in motility and polarization of entamoeba histolytica cells. | entamoeba histolytica, an enteric protozoan parasite, infects 10% of the world's population leading to 50 million cases of invasive amoebiasis annually. motility, which requires cell polarization, is important to the virulence of this pathogen, as it may result in destruction of host tissues and invasion. to gain insight into these processes in entamoeba, a unique rab gtpase, ehraba, which localizes to the leading edge of cells, was characterized. cell lines expressing a dominant negative versio ... | 2005 | 15760656 |
| in vitro antiprotozoal activity from the roots of geranium mexicanum and its constituents on entamoeba histolytica and giardia lamblia. | this paper examines the antiprotozoal activity of the dichloromethane-meoh extract, fractions and pure compounds from the roots of geranium mexicanum on entamoeba histolytica and giardia lamblia. the result indicated that the extract, organic fraction and a pure flavonoid were active against both protozoa with ic50 values ranging from 1.9 to 79.2 microg/ml for entamoeba histolytica and from 1.6 to 100.4 microg/ml in the case of giardia lamblia. the main active compound was the flavan-3-ol, (-)-e ... | 2005 | 15763382 |
| [infective etiology of diarrhea in adults with hiv infection in dakar: a case-control study on 594 patients]. | a survey was conducted in dakar, senegal from may 1997 to may 1999, to identify major types and prevalences of bacteria, parasites, fungi and rotaviruses associated with diarrhea in relation to human immunodeficiency virus serostatus with the aim to provide guidance to physicians for case management. etiologic agents were identified in a case-control study: cases were hiv-infected patients with diarrhea (hiv+ d+) and hiv seronegative patients with diarrhea (hiv- d+); controls were hiv-infected p ... | 2001 | 15773157 |
| effect of zinc-treated entamoeba histolytica on the human polymorphonuclear respiratory burst. | one of the mechanisms that entamoeba histolytica uses to evade host immune response is inhibition of the polymorphonuclear (pmn) leukocyte respiratory burst. in studies previously conducted in a model used in our laboratory, we observed that when treating trophozoites with different zinc concentrations certain amebic functions are inhibited while significantly limiting development of hepatic abscess in golden hamsters (mesocricetus aureatus). we carried out an in vitro study using a chemolumines ... | 2005 | 15778000 |
| nadph oxidase-derived reactive oxygen species-mediated activation of erk1/2 is required for apoptosis of human neutrophils induced by entamoeba histolytica. | the extracellular tissue penetrating protozoan parasite entamoeba histolytica has been known to induce host cell apoptosis. however, the intracellular signaling mechanism used by the parasite to trigger apoptosis is poorly understood. in this study, we investigated the roles of reactive oxygen species (ros), and of mapks in the entamoeba-induced apoptosis of human neutrophils. the neutrophils incubated with live trophozoites of e. histolytica revealed a marked increase of receptor shedding of cd ... | 2005 | 15778391 |
| pulmonary amoebiasis presenting as superior vena cava syndrome. | pulmonary amoebiasis without liver involvement occurs sporadically as a result of haematogenous spread from a primary site, the colon. the case history is presented of a patient who developed superior vena cava syndrome due to a pulmonary amoebic abscess without liver involvement. he was initially suspected of having a neoplasm but a combination of tests including histological examination of the h&e stained excised tissue, immunofluorescence using anti-entamoeba histolytica antibodies, and serol ... | 2005 | 15790993 |
| entamoeba histolytica activates host cell caspases during contact-dependent cell killing. | entamoeba histolytica is a human intestinal parasite that causes amoebic colitis as well as liver abscesses. host tissues are damaged through a three-step process involving adherence, contact-dependent cytolysis, and phagocytosis. these three processes all contribute to the pathogenicity of this parasite. adherence is provided by the gal/galnac adherence lectin. host cells are lysed in a contact-dependent fashion. there is evidence that suggests that this contact-dependent killing involves the i ... | 2005 | 15791956 |
| glycolysis in entamoeba histolytica. biochemical characterization of recombinant glycolytic enzymes and flux control analysis. | the synthesis of atp in the human parasite entamoeba histolytica is carried out solely by the glycolytic pathway. little kinetic and structural information is available for most of the pathway enzymes. we report here the gene cloning, overexpression and purification of hexokinase, hexose-6-phosphate isomerase, inorganic pyrophosphate-dependent phosphofructokinase, fructose-1,6 bisphosphate aldolase (aldo), triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase (gapdh), phosphoglycer ... | 2005 | 15794763 |
| identification of trypanothione from the human pathogen entamoeba histolytica by mass spectrometry and chemical analysis. | in this paper, we present definitive data to show, from esi (electrospray ionization) studies, that the thiol-bimane compound isolated and purified from entamoeba histolytica trophozoites, corresponds unequivocally to the structure of trypanothione. trypanothione disulphide was shown to have a molecular ion of m/z 722. it was further demonstrated by maldi-tof (matrix-assisted laser desorption ionization-time-of-flight) ms that this thiol compound also corresponds to the characteristic monoproton ... | 2005 | 15801913 |
| [presence of entamoeba histolytica in chronic urethritis]. | | 2005 | 15802118 |
| the pathogenicity of entamoeba histolytica is related to the capacity of evading innate immunity. | the host and parasite factors that influence susceptibility to entamoeba histolytica infection and disease are not well understood. entamoeba histolytica pathogenicity has been considered by focusing principally on parasite rather than host factors. thus, research has concentrated on explaining the molecular differences between pathogenic e. histolytica and non-pathogenic e. dispar. however, the amoeba molecules considered most important for host tissue destruction (amoebapore, galactose/n-acety ... | 2005 | 15813717 |
| cytokine mrna expressions in symptomatic vs. asymptomatic amoebiasis patients. | infection with entamoeba histolytica results in high mortality worldwide. studies on the cytokine response in symptomatic and asymptomatic amoebiasis (caused by e. histolytica, the pathogenic species and e. dispar, the non-pathogenic species) subjects and their correlation with symptomatology are lacking. the present study reports the cytokine response (tnf-alpha, ifn-gamma, il-4, il-10 and tgf-beta) in such subjects as measured by rt-pcr. the results showed significantly (< 0.05) higher express ... | 2005 | 15813721 |
| identification and gene expression analysis of a large family of transmembrane kinases related to the gal/galnac lectin in entamoeba histolytica. | we identified in the entamoeba histolytica genome a family of over 80 putative transmembrane kinases (tmks). the tmk extracellular domains had significant similarity to the intermediate subunit (igl) of the parasite gal/galnac lectin. the closest homolog to the e. histolytica tmk kinase domain was a cytoplasmic dual-specificity kinase, spla, from dictyostelium discoideum. sequence analysis of the tmk family demonstrated similarities to both serine/threonine and tyrosine kinases. tmk genes from e ... | 2005 | 15821132 |
| proteomic analysis of phagocytosis in the enteric protozoan parasite entamoeba histolytica. | proteomic analysis of phagosomes isolated from entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton rearrangement, vesicular trafficking, and degradation. phagosome localization of representative proteins was verified by immunofluorescence assay. this study should provide a basis for molecular identification and characterization of phagosome biogenesis. | 2005 | 15821141 |
| transcriptional profiling of entamoeba histolytica trophozoites. | we have developed an entamoeba histolytica genomic dna microarray and used it to develop a transcriptional profile of 1,971 e. histolytica (hm-1:imss) genes. the arrays accurately detected message abundance and 31-47% of amebic genes were expressed under standard tissue culture conditions (levels detectable by northern blot analysis or rt-pcr respectively). genes expressed at high levels ( approximately 2% of total) included actin (8.m00351), and ribosomal genes (20.m00312). moderately expressed ... | 2005 | 15826645 |
| [re-staining of the slides of entamoeba histolytica trophozoites]. | | 2004 | 15830894 |
| specificity determinants in inositol polyphosphate synthesis: crystal structure of inositol 1,3,4-trisphosphate 5/6-kinase. | inositol hexakisphosphate and other inositol high polyphosphates have diverse and critical roles in eukaryotic regulatory pathways. inositol 1,3,4-trisphosphate 5/6-kinase catalyzes the rate-limiting step in inositol high polyphosphate synthesis in animals. this multifunctional enzyme also has inositol 3,4,5,6-tetrakisphosphate 1-kinase and other activities. the structure of an archetypal family member, from entamoeba histolytica, has been determined to 1.2 a resolution in binary and ternary com ... | 2005 | 15837423 |
| [evaluation of the activity of polymorphonuclear neutrophils against entamoeba histolytica antigens]. | this paper evaluates the production of superoxide as an indicator of the activity of neutrophils from healthy individuals, stimulated with entamoeaba histolytica membrane antigens, chemical stimulators and antigens from other parasites. the kinetic nitroblue tetrazolium reduction test was used. no significant differences were observed in the control group as to superoxide production by neutrophils primed with entamoeba histolytica antigens or cisticercus cellulosae, toxoplasma gondii and giardia ... | 2002 | 15849934 |
| [antiamebic effect of metronidazole proved in a study conducted in cienfuegos province]. | three studies were conducted in cienfuegos province to prove that intestinal amebiasis in cuba could be an overestimated health problem. the first two studies showed two components of this overestimation: microscopical overdiagnosis and lack of knowledge about entamoeba dispar, a non-pathogenic species, in most cases in which microscopical examination was correct. this paper reported the third study that showed the third component: the wrong belief that entamoeba histolytica is resistant to metr ... | 2002 | 15849935 |
| a novel adp/atp transporter in the mitosome of the microaerophilic human parasite entamoeba histolytica. | recent data suggest that microaerophilic and parasitic protozoa, which lack oxidative phosphorylation, nevertheless contain mitochondrial homologs [1-6], organelles that share common ancestry with mitochondria. such widespread retention suggests there may be a common function for mitochondrial homologs that makes them essential for eukaryotic cells. we determined the mitochondrial carrier family (mcf) complement of the entamoeba histolytica mitochondrial homolog, also known as a crypton [5] or m ... | 2005 | 15854906 |
| [determination of entamoeba histolytica infection in patients from greater metropolitan belém, para, brazil, by enzyme-linked immunosorbent assay (elisa) for antigen detection]. | the epidemiological status of amebiasis has been reevaluated since entamoeba histolytica (pathogenic) was considered a distinct species from entamoeba dispar (non-pathogenic). we investigated e. histolytica antigens in stool samples from residents of belém, para state, brazil, with commercially available enzyme-linked immunosorbent assay (e. histolytica test, techlab inc., blacksburg, usa). a total of 845 samples were analyzed, of which 248 were positive (29.35%). e. histolytica infection was mo ... | 2005 | 15868057 |
| entamoeba histolytica: molecular characterization of an aldose 1-epimerase (mutarotase). | in cells, the alpha-anomers of aldoses are the preferred metabolizable substrates, while beta-anomers of aldoses play their role in glycan structure. in the cytoplasm, alpha- and beta-anomers of aldoses interconvert through the enzyme termed aldose 1-epimerase or mutarotase (ec 5.1.3.3). we have identified a mutarotase gene in entamoeba histolytica, the causative agent of non-bacterial dysentery in humans. cloning and characterization of this gene in two strains of the parasite (hm-1:imss and ra ... | 2005 | 15869755 |
| risk factors for infection by the entamoeba histolytica/e. dispar complex: an epidemiological study conducted in outpatient clinics in the city of manaus, amazon region, brazil. | an epidemiological study was conducted on a population attending outpatient clinics in manaus, amazon, brazil to determine the prevalences of infection by the entamoeba histolytica/e. dispar complex and by e. histolytica alone, as well as to identify the risk factors involved in transmission. the study was conducted in two phases: survey and case-control. face-to-face interviews were carried out and faecal samples collected from 1578 individuals. faeces were examined by optical microscopy and te ... | 2005 | 15869773 |
| real-time-pcr assay for diagnosis of entamoeba histolytica infection. | we developed a real-time-pcr assay utilizing a molecular-beacon probe for the detection of entamoeba histolytica and compared its sensitivity to stool antigen detection and traditional pcr. a total of 205 stool and liver abscess pus specimens from patients and controls were used for this purpose, 101 (49%) of which were positive by the techlab e. histolytica-specific antigen detection test, while the other 104 (51%) stool and liver abscess pus specimens were negative by the antigen detection tes ... | 2005 | 15872237 |
| amebic liver abscesses among neonates can mimic bacterial sepsis. | amebic liver abscesses are unusual among neonates. three (3.6%) of 83 children who presented to a university hospital with amebic liver abscesses in a 17-year period were neonates (<28 days of age). patients presented with nonspecific clinical and laboratory findings mimicking fulminant neonatal sepsis. diagnosis was delayed and was based on the presence of a hypoechoic lesion in the liver, antibodies against entamoeba histolytica and microbiologic examination of pus. in addition to parenteral m ... | 2005 | 15876952 |
| entamoeba histolytica or entamoeba dispar among children in gaza, gaza strip? | most physicians in gaza prescribe medicaments for patient's suffering from entamoeba histolytica/dispar without parasitologic diagnosis. additionally, stool analysis performed by the routine methods usually reports the species as e. histolytica without con-firmation. in this study, 92 stool specimens were collected and analyzed by wet mount, iron haematoxylin staining, antigen detection of e. histolytica and polymerase chain reaction (pcr). the total number of e. histolytica identified by pcr wa ... | 2005 | 15880995 |
| entamoeba histolytica ehpgp5 transcriptional activation depends on putative emetine response elements. | the multidrug resistance ehpgp5 gene promoter is active in drug resistant clone c2 trophozoites and its activity increases when trophozoites are cultured in the presence of emetine, suggesting that the ehpgp5 gene shows an inducible drug dependent mechanism. we analyzed different promoter fragments to detect those regions that activate transcription in the presence of emetine. trophozoites were transfected with p375pgp5, p259pgp5, p187pgp5, and p76pgp5 plasmids and incubated with different emeti ... | 2005 | 15893309 |
| unexpected diversity and differential success of dna transposons in four species of entamoeba protozoans. | we report the first comprehensive analysis of transposable element content in the compact genomes (approximately 20 mb) of four species of entamoeba unicellular protozoans for which draft sequences are now available. entamoeba histolytica and entamoeba dispar, two human parasites, have many retrotransposons, but few dna transposons. in contrast, the reptile parasite entamoeba invadens and the free-living entamoeba moshkovskii contain few long interspersed elements but harbor diverse and recently ... | 2005 | 15901838 |
| prevalence of intestinal parasites among members of the public in kuala lumpur, malaysia. | a total of 246 stool samples were collected from the public who participated in a medical fair held at the university malaya medical center. the stools were examined for intestinal parasites using the formalin-ether concentration technique. the overall infection rate was 6.9% (17 out of 246), with trichuris trichiura being the most common parasite (4.5%), followed by ascaris lumbricoides (0.8%), clonorchis sinensis (0.8%), hookworm (0.4%), and entamoeba histolytica (0.4%). none of these particip ... | 2005 | 15906644 |
| entamoeba histolytica and entamoeba dispar utilize externalized phosphatidylserine for recognition and phagocytosis of erythrocytes. | amebic erythrophagocytosis is characteristic of invasive amebiasis, and mutants deficient in erythrocyte ingestion are avirulent. we sought to understand the molecular mechanisms underlying erythrocyte phagocytosis by entamoeba histolytica. following adherence to amebae, erythrocytes became round and crenulated, and phosphatidylserine (ps) was exposed on their outer membrane leaflets. these changes were similar to the effects of calcium treatment on erythrocytes, which we utilized to separate am ... | 2005 | 15908370 |
| entamoeba histolytica-secreted products degrade colonic mucin oligosaccharides. | degradation of the mucus layer by entamoeba histolytica is a prerequisite for invasion of the colonic mucosa. in this study, we demonstrate that amoeba-secreted products degrade (3)h-labeled and native colonic mucin oligosaccharides independently of proteolytic activity. we conclude that e. histolytica degrades mucin oligosaccharides, which may facilitate parasite invasion of the colon. | 2005 | 15908414 |
| the innate immune response to entamoeba histolytica lipopeptidophosphoglycan is mediated by toll-like receptors 2 and 4. | entamoeba histolytica is a human pathogen that may invade the intestinal mucosa, causing amoebic colitis or hepatic abscesses when the trophozoites travel through the portal circulation to the liver. lipopeptidophosphoglycan (lppg) is a molecular pattern of e. histolytica recognized by the human immune system. here we report that lppg is exposed on the cell surface of e. histolytica trophozoites, and is recognized by the host through toll-like receptor (tlr) 2 and tlr4. correspondingly, human em ... | 2005 | 15910421 |
| synthesis and antiamoebic activity of 3,7-dimethyl-pyrazolo[3,4-e][1,2,4] triazin-4-yl thiosemicarbazide derivatives. | a series of 3,7-dimethyl-pyrazolo[3,4-e][1,2,4]triazin-4-yl thiosemicarbazide derivatives 3-22 were prepared and evaluated in vitro against hm1:1mss strain of entamoeba histolytica, to identify the compounds for antiamoebic activity. they exhibited antiamoebic activity in the range (ic50=0.81-7.31microm). the results were compared to the activity of known drug metronidazole. it is inferred from the in vitro studies that the compounds 10, 11, 17 and 18 were found to be significantly better inhibi ... | 2005 | 15911221 |
| entamoeba histolytica: biochemical and molecular insights into the activities within microsomal fractions. | one of the most fascinating aspects of the entamoeba histolytica trophozoite ultrastructure is the lack of a typical secretory pathway, particularly of rough endoplasmic reticulum and golgi system, in a cell with such a high secretory activity. here, we describe the isolation of amoeba cell structures containing er-typical activities. following isopycnic centrifugation of plasma membrane-free extracts, microsomes enriched in enzymatic activities such as dolichol-p-mannose synthase (dpms; ec 2.4. ... | 2005 | 15913610 |
| prevalence and risk factors for protozoan and nematode infections among children in an ecuadorian highland community. | in order to create a parasite prevention program for santa ana, ecuador, we worked with local physicians to identify the prevalence and risk factors for intestinal parasitic infection among children. two hundred households were randomly selected and asked to submit three stool samples from a selected child. information from direct observation and questionnaires was used to determine environmental and behavioral risk factors for parasitic infection. prevalence of any parasitic infection was 65.6% ... | 2005 | 15916785 |
| 1-n-substituted thiocarbamoyl-3-phenyl-2-pyrazolines: synthesis and in vitro antiamoebic activities. | the title compounds were prepared by reaction of mannich bases with various n-4 substituted thiosemicarbazides. the chemical structures of the compounds were proved by means of their uv, ir, 1h nmr, 13c nmr spectroscopic data and elemental analyses. the in vitro antiamoebic activities of these compounds were evaluated by microdilution method against hm1:imss strain of entamoeba histolytica and compared with the standard drug, metronidazole. it was concluded that 3-chloro and 3-bromo substituents ... | 2005 | 15922492 |
| novel bidentate complexes of cu(ii) derived from 5-nitrofuran-2-carboxaldehyde thiosemicarbazones with antiamoebic activity against e. histolytica. | the novel analogues of 5-nitrofuran-2-carboxaldehyde thiosemicarbazones 1-10 were synthesized and their copper(ii) complexes 1a-10a were obtained by means of coordination with cupric chloride. all these compounds have been characterized by elemental analysis, ir, electronic spectra and thermogravimetric patterns while ligands have also been characterized by 1h nmr spectral studies. these copper complexes are bidentate and possess octahedral geometry around cu(ii) ion. their antiamoebic activitie ... | 2005 | 15922839 |
| experience with aspiration in cases of amebic liver abscess in an endemic area. | the study presented here was performed to evaluate the need for aspiration in patients with amebic liver abscess (ala). patients older than 12 years with a diagnosis of ala based on clinical features, ultrasound results, and positive amebic serology were included in the study (n=144). serological testing was performed to detect the presence of immunoglobin g antibody against entamoeba histolytica, and a value of more than 0.4 optical density units was considered positive. all patients were given ... | 2005 | 15928909 |
| sequence specific 1hn, 13c and 15n resonance assignments of a novel calcium-binding protein from entamoeba histolytica. | | 2005 | 15929013 |
| microorganisms and parasites in chronic infective diarrhea. | to reveal the pattern of microorganisms in chronic infective diarrhea cases. | 2004 | 15931720 |
| protective activity of picroliv on hepatic amoebiasis associated with carbon tetrachloride toxicity. | picroliv, isolated from the root and rhizome of picrorhiza kurroa, is known to have significant hepatoprotective activity. its effects against entamoeba histolytica induced liver damage are not studied. this study aims to evaluate the hepatoprotective action of picroliv against the hepatotoxic changes induced by carbon tetrachloride (ccl(4)) and e. histolytica infection in three animal models. | 2005 | 15937372 |
| [value of screening for intestinal and urinary parasites in non-resident permanent students in tunisia]. | in order to fight digestive and urinary parasitoses, a national program of surveillance has been founded. the screening of these parasitoses among the non permanent resident students in tunisia (enrpts) is one of the main actions of this program. among 2560 enrpts tested in the laboratory of parasitologie of institut pasteur of tunis, between 1998 and 2002, 674 were infected by parasites, which represents a global prévalence of 26.3%. the intestinal protozoa constitute the majority of parasites ... | 2003 | 15941065 |
| genome update: prediction of secreted proteins in 225 bacterial proteomes. | | 2005 | 15941981 |
| entamoeba histolytica: observations on metabolism based on the genome sequence. | the sequencing of the genome of entamoeba histolytica has allowed a reconstruction of its metabolic pathways, many of which are unusual for a eukaryote. based on the genome sequence, it appears that amino acids may play a larger role than previously thought in energy metabolism, with roles in both atp synthesis and nad regeneration. arginine decarboxylase may be involved in survival of e. histolytica during its passage through the stomach. the usual pyrimidine synthesis pathway is absent, but a ... | 2005 | 15955308 |
| entamoeba histolytica: construction and applications of subgenomic databases. | knowledge about the influence of environmental stress such as the action of chemotherapeutic agents on gene expression in entamoeba histolytica is limited. we plan to use oligonucleotide microarray hybridization to approach these questions. as the basis for our array, sequence data from the genome project carried out by the institute for genomic research (tigr) and the sanger institute were used to annotate parts of the parasite genome. three subgenomic databases containing enzymes, cytoskeleton ... | 2005 | 15955309 |
| entamoeba histolytica: comparative genomics of the pre-mrna 3' end processing machinery. | we report here the pre-mrna 3' end processing machinery in entamoeba histolytica. comparative analysis of the putative sequences participating in the pre-mrna 3' end processing of e. histolytica genes shows similitude and differences to those described for yeast and human transcripts. by a genomic survey, we identified 16 putative genes encoding for cleavage/polyadenylation factors in this parasite. e. histolytica pre-mrna 3' end processing machinery does not seem to contain homologous genes cod ... | 2005 | 15955310 |
| entamoeba histolytica: analysis of the trophozoite proteome by two-dimensional polyacrylamide gel electrophoresis. | the entamoeba histolytica genome project carried out at tigr and the sanger institute has produced a near-complete set of deduced open reading frame sequences. these data provide strong support for the identification of signals from proteomic analyses such as two-dimensional electrophoresis by protein sequencing and/or mass spectrometric methods. to carry out an initial investigation of the e. histolytica proteome, appropriate sample preparation and silver staining protocols were adapted. after ... | 2005 | 15955311 |
| genomic dna microarrays for entamoeba histolytica: applications for use in expression profiling and strain genotyping. | the parasite entamoeba histolytica is a causative agent of dysentery and liver abscesses. found predominantly in developing countries, this parasitic infection is responsible for significant morbidity and mortality. we have developed a genomic dna microarray for e. histolytica. the array composed of 11,328 clones contains >2000 unique genes and was utilized for expression profiling and comparative genomic hybridizations of entamoeba strains. we present a synopsis of our results to date and poten ... | 2005 | 15955312 |
| the lines and sines of entamoeba histolytica: comparative analysis and genomic distribution. | autonomous non-long terminal repeat retrotransposons are commonly referred to as long interspersed elements (lines). short non-autonomous elements that borrow the line machinery are called sines. the entamoeba histolytica genome contains three classes of lines and sines. together the ehlines/sines account for about 6% of the genome. the recognizable functional domains in all three ehlines included reverse transcriptase and endonuclease. a novel feature was the presence of two types of members-so ... | 2005 | 15955314 |
| entamoeba histolytica: characterization of human collagen type i and ca2+ activated differentially expressed genes. | earlier it was demonstrated that the entamoeba histolytica trophozoites, when incubated with human collagen and ca2+, expressed and released the collagenolytic activity [munoz, m.l., calderon, j., rojkind, m., 1982. the collagenase of entamoeba histolytica. journal of experimental medicine 155, 42-51], a virulence factor involved in the pathogenesis of amoebiasis. in this study, attempts have been made to identify and characterize the gene(s) that are upregulated by the human collagen type i and ... | 2005 | 15955315 |
| proteomic analysis of gal/galnac lectin-associated proteins in entamoeba histolytica. | contact-dependent killing and phagocytosis of target cells by entamoeba histolytica trophozoites is mediated by the galactose (gal) and n-acetyl-d-galactosamine (galnac)-inhibitable lectin. previous work has suggested that this lectin functions as part of a signal transduction complex. to identify proteins that might be part of this complex, amebic trophozoites were bound to galnac-bsa-labeled magnetic beads and lysed. bound proteins were eluted from the beads and analyzed by tandem mass spectro ... | 2005 | 15955316 |
| entamoeba histolytica: cloning and expression of the poly(a) polymerase ehpap. | in eukaryotes, polyadenylation of pre-mrna 3' end is essential for mrna export, stability, and translation. here we identified and cloned a gene codifying for a putative nuclear poly(a) polymerase (ehpap) in entamoeba histolytica. protein sequence alignments with eukaryotic paps showed that ehpap has the rna-binding region and the pap central domain with the catalytic nucleotidyl transferase domain described for other nuclear paps. recombinant ehpap expressed in bacteria was used to generate spe ... | 2005 | 15955317 |
| entamoeba histolytica: functional characterization of the -234 to -196 bp promoter region of the multidrug resistance ehpgp1 gene. | the multidrug resistance ehpgp1 gene is constitutively expressed in drug resistant trophozoites from entamoeba histolytica. it has been demonstrated that two ccaat/enhancer binding sites located in the ehpgp1 gene promoter control its transcriptional activation. however, functional assays of the 5' end of its promoter showed that region from -234 to -196 bp (38 bp) is also important for the ehpgp1 gene transcription. here, we demonstrated that in the 38 bp region putative cis-activator sequences ... | 2005 | 15955318 |
| the diversity of rab gtpases in entamoeba histolytica. | rab proteins are ubiquitous small gtp-binding proteins that form a highly conserved family and regulate vesicular trafficking. recent completion of the genome of the enteric protozoan parasite entamoeba histolytica enabled us to identify an extremely large number (>90) of putative rab genes. multiple alignment and phylogenic analysis of amebic, human, and yeast rab showed that only 22 amebic rab proteins including ehrab1, ehrab2, ehrab5, ehrab7, ehrab8, ehrab11, and ehrab21 showed significant si ... | 2005 | 15955319 |
| entamoeba histolytica: identification of ehgpcr-1, a novel putative g protein-coupled receptor that binds to ehrabb. | ehrabb is an entamoeba histolytica protein involved in phagocytosis. however, proteins that regulate the ehrabb activity are unknown. here, we report the identification of a putative g protein-coupled receptor of e. histolytica (ehgpcr-1) that binds to ehrabb. by two-hybrid screening, we found a 372-bp cdna fragment that encodes the c-terminus of ehgpcr-1. the cloning and sequence of the full-length cdna revealed that it predicts a polypeptide with two tyrosine-based sorting signals for endocyto ... | 2005 | 15955320 |
| entamoeba histolytica: alterations in ehrabb protein in a phagocytosis deficient mutant correlate with the entamoeba dispar rabb sequence. | we analyzed the expression and location of ehrabb in clone l-6, a phagocytosis-deficient mutant of entamoeba histolytica, in comparison with the wild-type clone a. intriguingly, trophozoites of clone l-6 express more ehrabb than those of clone a. however, the majority of ehrabb-containing vesicles remained in the cytoplasm of clone l-6 during phagocytosis. to investigate molecular alterations in ehrabb of clone l-6 we compared the ehrabb gene sequences from clones l-6 and a. we also isolated, se ... | 2005 | 15955321 |
| molecular characterization of entamoeba histolytica rnase iii and ago2, two rna interference hallmark proteins. | entamoeba histolytica, a protozoan parasite with variable dna content and complex ploidity, has defied most efforts aimed at gene depletion using classical genetic methods. in this study, we identified and characterized two proteins involved in the rna interference (rnai) pathway, rnase iii and ago2. our results strengthen the findings that an rnai pathway does exist in this parasite. | 2005 | 15955322 |
| a novel protease from entamoeba histolytica homologous to members of the family s28 of serine proteases. | serine proteases are one of the biologically most important and widely distributed enzyme families. a protease capable of degrading the substrate suc-aaf-amc was isolated from axenically grown trophozoites of entamoeba histolytica. the enzyme was purified by ion-exchange chromatography and electroelution, and appeared on 2d-page as a spot of 60 kda and pi of 4.65. data obtained from zymogram suggest the active protease is present either as homodimer (130 kda) or homotetramer (250 kda). the optim ... | 2005 | 15955323 |
| entamoeba histolytica: identification and characterization of an n-ethylmaleimide sensitive fusion protein homologue. | entamoeba histolytica is a phagocytic cell with numerous vesicles of different sizes and shapes but without a well-defined golgi apparatus. despite this, genes implied in membrane trafficking have been identified in the genome of this parasite. one of these genes is homologous to the n-ethylmaleimide sensitive fusion factor (nsf), whose protein has been shown to play an important role in vesicle fusion in other eukaryotic cells. in this report, we investigated the nsf homologue gene from a patho ... | 2005 | 15955324 |
| entamoeba histolytica: structural and functional analysis of the ehadh112 gene promoter. | the entamoeba histolytica ehcp112 and ehadh112 genes that encode the ehcpadh complex are separated by a non-coding 188pb region. their proximity suggests a coordinated expression regulation for both genes. here, we studied the structure and function of 996 bp (p996cat) upstream the atg start codon of the ehadh112 gene. the p996cat plasmid drove cat transcription with a 78% of the activity showed by actin promoter. deletion of 330 bp at the 5' end of p966cat to produce the p776cat plasmid, decrea ... | 2005 | 15955325 |
| the entamoeba histolytica ehcp112 gene has a distal and weak promoter. | ehcp112 encodes the entamoeba histolytica ehcp112 cysteine protease that is part of the ehcpadh complex. by in silico analysis we identified putative transcription factor-binding sites along 837 bp upstream the ehcp112 gene atg codon. a tata-like motif (tatataaa) was located at -36 to -29 bp, a gaac box (gaacc) was found at -10 to -14 bp and an inr sequence (ttcaac) at -8 to -2 bp. these tripartite promoter elements are in non-canonical positions, downstream the transcription initiation site (-2 ... | 2005 | 15955326 |
| functional characterization of ehadh112: an entamoeba histolytica bro1 domain-containing protein. | ehadh112 is part of the ehcpadh complex, a protein involved in key events of the entamoeba histolytica host invasion. ehadh112 participates in trophozoite adherence to target cells and in phagocytosis. we report here the finding of two ehadh112 homologues in the e. histolytica genome (ehadh112-like proteins). ehadh112 and its relatives have a bro1 domain at their amino-terminus and a consensus context for phosphorylation by src-tyrosine kinases, both involved in signal transduction processes in ... | 2005 | 15955327 |
| species- and strain-specific probes derived from repetitive dna for distinguishing entamoeba histolytica and entamoeba dispar. | entamoeba histolytica and entamoeba dispar are two morphologically indistinguishable species that are found in the human gut. of the two, e. histolytica is considered to be pathogenic while e. dispar is nonpathogenic. to generate molecular probes to detect and distinguish between the two species, we utilized repeat sequences present in entamoeba genome. we have developed probes and primers from rdna episomes, and unidentified entamoeba est1 repeat for this purpose, and used them for dot blot hyb ... | 2005 | 15955328 |
| entamoeba histolytica: mechanism of decrease of virulence of axenic cultures maintained for prolonged periods. | intraportal injection of non-virulent e. histolytica (derived from prolonged axenic culture of virulent e. histolytica) strain hm1-imss in normal hamsters results in no liver lesions and disappearance of the parasites 48-72 h after injection. viability of non-virulent e. histolytica after 2 h of in vitro incubation in either fresh or decomplemented hamster serum is the same as control virulent e. histolytica (50-90%). in hamsters made leukopenic, or both leukopenic+hypocomplementemic, or hypocom ... | 2005 | 15955329 |
| entamoeba histolytica: the serine-rich gene polymorphism-based genetic variability of clinical isolates from georgia. | it is generally accepted that a majority of individuals infected by entamoeba histolytica do not develop symptomatic disease. however, the parasite and the host factors contributing to the development of the disease, remain undetermined. it is also unclear why certain individuals develop extra-intestinal amebiasis without exhibiting apparent intestinal symptoms. an outbreak of amebic liver abscess in tbilisi, georgia in 1998-1999 suggested that the causative e. histolytica strain had an unusual ... | 2005 | 15955330 |
| entamoeba invadens: in vitro axenic encystation with a serum substitute. | the current media for axenic cultivation of entamoeba histolytica and entamoeba invadens are supplemented with bovine or equine serum, which provides several essential nutrients to amoebas. serum has also been considered an essential component in encystation media for e. invadens. a substitute of serum, pacsr has been described as an alternative for growth of e. histolytica and also maintains growth of e. invadens. when pacsr was used instead of serum for encystation of e. invadens the efficienc ... | 2005 | 15955331 |
| comparison of two methods (microscopy and enzyme-linked immunosorbent assay) for the diagnosis of amebiasis. | diagnosis of amebiasis is usually performed on a clinical basis alone in most endemic countries having limited economic resources. this epidemiological study was conducted using modern diagnostic tests for amebiasis in the southeastern region of turkey, an endemic area for amebiasis. the population of this study included patients with symptomatic diarrhea/dysentery attending both yuzuncu yil university, van and harran university, sanliurfa, turkey. a total of 380 stool specimens were collected a ... | 2005 | 15955332 |
| entamoeba histolytica and entamoeba dispar: prevalence infection in a rural mexican community. | the frequency of entamoeba histolytica and entamoeba dispar infection was analyzed in a rural community in the state of morelos, mexico, through pcr technique by using specie specific primer. the e. histolytica specie was detected in 33 of 290 analyzed stool samples (11.4%), e. dispar specie was observed in 21 samples (7.2%) and both species of entamoeba were detected in seven samples (2.4%). so a higher e. histolytica than e. dispar frequency infection was detected (13.8 versus 9.6%). even thou ... | 2005 | 15955333 |
| entamoeba histolytica and/or entamoeba dispar: infection frequency in hiv+/aids patients in mexico city. | the objective of this work was to evaluate the frequency of entamoeba histolytica/entamoeba dispar intestinal infection in hiv+/aids subjects and their hiv- close relatives or sexual partners. enteric parasites were investigated in stool samples by microscopic examination and e. histolytica and e. dispar were identified by pcr. we found by microscopic analysis in hiv+/aids group that the e. histolytica/e. dispar complex was present in 5.9% of the members, while in the hiv- group was 2.9%. with p ... | 2005 | 15955334 |
| diarrheal illness among deployed u.s. military personnel during operation bright star 2001--egypt. | in the fall of 2001, approximately 15,000 u.s. military personnel participated in a military exercise in the northwestern egyptian desert. to assess the prevalence and impact of diarrhea and enteropathogen distribution, we conducted a post-deployment survey and a case series study. a departure convenience sampling (n = 3725) was used in the post-deployment survey. overall, 9.3% reported diarrhea, 2.6% sought medical care, and 2.8% stopped or decreased their work for at least a day. among those r ... | 2005 | 15964494 |
| expression of inducible nitric oxide synthase mrna and nitric oxide production during the development of liver abscess in hamster inoculated with entamoeba histolytica. | the present study analyzed inos and enos mrna expression and no production during development of hepatic abscess caused by entamoeba histolytica trophozoites. one 374-bp sequence, which displayed 88% identity to mammalian inos protein, was isolated from lps-stimulated peritoneal hamster macrophages. a separate 365-bp cdna sequence showed 99% identity with enos protein. inos mrna was detected in hamsters during formation of amoebic liver abscesses, but not in control hamsters. enos mrna expressio ... | 2005 | 15968502 |
| in vitro antiprotozoal activity of the leaves of artemisia ludoviciana. | the inhabitants of northeast of mexico use an infusion of leaves from artemisia ludoviciana as an antidiarrheal remedy. the aqueous, methanol, acetone and hexane leaf extracts from mature plants were found to be active in vitro against the parasitic protozoa entamoeba histolytica and giardia lamblia. | 2005 | 15975735 |
| [intestinal and extraintestinal amebiasis: clinical notes]. | | 2005 | 15984611 |
| identification of an entamoeba histolytica gene encoding a protein disulfide isomerase that functionally complements the dsba mutation in escherichia coli. | | 2005 | 15990181 |
| entamoeba histolytica and entamoeba dispar infections in cynomolgus monkeys imported into japan for research. | three hundred and three stool samples of cynomolgus monkeys (macaca fascicularis) imported from china and the philippines were examined for entamoeba histolytica/entamoeba dispar infections. microscopy detected e. histolytica/e. dispar cysts in 41 samples. positive rates were higher in the monkeys from china (37.5%) than in the monkeys from the philippines (3.7%). pcr analysis of 25 samples successfully cultured from the cysts demonstrated that 24 were e. dispar, one of the samples from china wa ... | 2005 | 15991045 |