| specific bacillus anthracis identification by a plcr-targeted restriction site insertion-pcr (rsi-pcr) assay. | a rsi-pcr assay was developed for the detection of a bacillus anthracis-specific nonsense mutation in the plcr gene. the assay specificity was tested using 170 bacillus spp. strains including 47 strains of b. anthracis. the plcr rsi-pcr distinguished bacillus cereus group strains closely related to b. anthracis from the anthrax agent. the assay was found to be a robust, simple and cost effective tool for b. anthracis identification. in contrast to previously developed real time pcr-based methods ... | 2007 | 17490431 |
| crystal structures of human dipeptidyl peptidase iv in its apo and diprotin b-complexed forms. | dipeptidyl peptidase iv (dppiv), which belongs to the prolyl oligopeptidase family of serine proteases, is known to have a variety of regulatory biological functions and has been shown to be implicated in type 2 diabetes. it is therefore important to develop selective human dppiv (hdppiv) inhibitors. in this study, we determined the crystal structure of apo hdppiv at 1.9 a resolution. our high-resolution crystal structure of apo hdppiv revealed the presence of sodium ion and glycerol molecules a ... | 2007 | 17492130 |
| low concentrations of bile salts induce stress responses and reduce motility in bacillus cereus atcc 14579 [corrected]. | tolerance to bile salts was investigated in forty bacillus cereus strains, including 17 environmental isolates, 11 dairy isolates, 3 isolates from food poisoning outbreaks, and 9 other clinical isolates. growth of all strains was observed at low bile salt concentrations, but no growth was observed on lb agar plates containing more than 0.005% bile salts. preincubation of the b. cereus type strain, atcc 14579, in low levels of bile salts did not increase tolerance levels. b. cereus atcc 14579 was ... | 2007 | 17496091 |
| a functional homing endonuclease in the bacillus anthracis nrde group i intron. | the essential bacillus anthracis nrde gene carries a self-splicing group i intron with a putative homing endonuclease belonging to the giy-yig family. here, we show that the nrde pre-mrna is spliced and that the homing endonuclease cleaves an intronless nrde gene 5 nucleotides (nt) upstream of the intron insertion site, producing 2-nt 3' extensions. we also show that the sequence required for efficient cleavage spans at least 4 bp upstream and 31 bp downstream of the cleaved coding strand. the p ... | 2007 | 17496101 |
| bacillus cereus var. toyoi enhanced systemic immune response in piglets. | probiotic bacteria have been suggested to stimulate the host immune system. in this study we evaluated the immunomodulatory effects of probiotic bacillus cereus var. toyoi on the systemic immunity of piglets. a pool of 70 piglets was divided into a probiotic or control group. we determined the ratios of peripheral blood mononuclear cell (pbmc) subsets and measured proliferative responses and cytokine production of pbmcs and effects on vaccination responses. blood samples of probiotic-treated pig ... | 2007 | 17498814 |
| new triazole and triazolothiadiazine derivatives as possible antimicrobial agents. | triazole and triazoles fused with six-membered ring systems are found to possess diverse applications in the fields of medicine, agriculture and industry. the new 1,2,4-triazole and 1,2,4-triazolo[3,4-b][1,3,4]thiadiazine derivatives were synthesized as novel antimicrobial agents. the reaction of 1h-indol-3-acetic acid with thiocarbohydrazide gave the 4-amino-3-mercapto-5-[(1h-indol-3-yl)methyl]-4h-1,2,4-triazole. the reaction of triazole with arylaldehydes in ethanol gave the 4-arylideneamino-3 ... | 2008 | 17499887 |
| crystal structure of the bczbp, a zinc-binding protein from bacillus cereus. | bacillus cereus is an opportunistic pathogenic bacterium closely related to bacillus anthracis, the causative agent of anthrax in mammals. a significant portion of the b. cereus chromosomal genes are common to b. anthracis, including genes which in b. anthracis code for putative virulence and surface proteins. b. cereus thus provides a convenient model organism for studying proteins potentially associated with the pathogenicity of the highly infectious b. anthracis. the zinc-binding protein of b ... | 2007 | 17501983 |
| simultaneous removal of chlorothalonil and nitrate by bacillus cereus strain ns1. | elevated no3- and chlorothalonil (ctn) have been found in production nursery recycling ponds. bacillus cereus strain ns1 isolated from nursery recycling pond sediment was assessed for its ability to reduce no3- and degrade ctn in a mineral medium. the results showed that the efficiency of no3- reduction and ctn degradation by b. cereus strain ns1 were related to the nature of organic carbon sources added to the medium. in the medium amended with 100 mg/l yeast extract, 86% of no3- (100 mg/l) and ... | 2007 | 17509667 |
| food poisoning due to jimson weed mimicking bacillus cereus food intoxication in austria, 2006. | | 2007 | 17512772 |
| toxin production in a rare and genetically remote cluster of strains of the bacillus cereus group. | three enterotoxins are implicated in diarrhoeal food poisoning due to bacillus cereus: haemolysin bl (hbl), non-haemolytic enterotoxin (nhe), and cytotoxin k (cytk). toxin gene profiling and assays for detection of toxin-producing stains have been used in attempts to evaluate the enterotoxic potential of b. cereus group strains. b. cereus strain nvh 391/98, isolated from a case of fatal enteritis, was genetically remote from other b. cereus group strains. this strain lacked the genes encoding hb ... | 2007 | 17517121 |
| bacillus cereus spores during housing of dairy cows: factors affecting contamination of raw milk. | the contamination of raw milk with bacillus cereus spores was studied during the indoor confinement of dairy cattle. the occurrence of spores in fresh and used bedding material, air samples, feed, feces, and the rinse water from milking equipment was compared with the spore level in bulk tank milk on 2 farms, one of which had 2 different housing systems. a less extensive study was carried out on an additional 5 farms. high spore concentrations of >100 spores/l in the raw milk were found on 4 of ... | 2007 | 17517714 |
| two distinct manganese-containing superoxide dismutase genes in bacillus cereus: their physiological characterizations and roles in surviving in wheat rhizosphere. | rhizosphere inhabitants interact intricately with plant host. bacillus cereus 905 isolated from wheat rhizosphere colonized wheat rhizosphere with large population size. in this work, the role of superoxide dismutases (sods) of b. cereus 905 in surviving in wheat rhizosphere was analyzed. two genes, soda-1 and soda-2 encoding two distinct manganese sods (mnsods), were isolated from the bacterium. the amino acid sequence similarity between the two peptides is 58.43%. through homologous recombinat ... | 2007 | 17521361 |
| [bacillus cereus enterotoxins]. | data about bacillus cereus different enterotoxins including hemolysin (hbl), nonhemolytic enterotoxin (nhe), enterotoxin (t), and emesis-inducing thermostable enterotoxin (ete) are summarized in the article. information about synthesis of different diarrhea-inducing and emesis-inducing enterotoxins, methods of their purification, structure, functions, and mechanisms of action are presented. commercial kits for identification of b. cereus enterotoxins causing food-associated diarrhea are listed. | 2007 | 17523492 |
| phenotypic and functional characterization of bacillus anthracis biofilms. | biofilms, communities of micro-organisms attached to a surface, are responsible for many chronic diseases and are often associated with environmental reservoirs or lifestyles. bacillus anthracis is a gram-positive, endospore-forming bacterium and is the aetiological agent of pulmonary, gastrointestinal and cutaneous anthrax. anthrax infections are part of the natural lifecycle of many ruminants in north america, including cattle and bison, and b. anthracis is thought to be a central part of this ... | 2007 | 17526827 |
| large-scale production and efficient recovery of phb with desirable material properties, from the newly characterised bacillus cereus spv. | a newly characterised bacillus strain, bacillus cereus spv was found to produce phb at a concentration of 38% of its dry cell weight in shaken flask cultures, using glucose as the main carbon source. polymer production was then scaled up to 20 l batch fermentations where 29% dry cell weight of phb was obtained within 48 h. following this, a simple glucose feeding strategy was developed and the cells accumulated 38% dry cell weight of phb, an increase in the overall volumetric yield by 31% compar ... | 2007 | 17532079 |
| differentiation and characterization by molecular techniques of bacillus cereus group isolates from poto poto and dégué, two traditional cereal-based fermented foods of burkina faso and republic of congo. | poto poto (a maize sourdough) and dégué (a pearl millet-based food) are two traditional african fermented foods. the molecular biology of toxigenic and pathogenic bacteria found in those foods is largely unknown. the purpose of this study was to study the phylogenetic relatedness and toxigenic potential of 26 bacillus cereus group isolates from these traditional fermented foods. the relatedness of the isolates was evaluated with repetitive element sequence-based pcr (rep-pcr) and 16s rdna sequen ... | 2007 | 17536675 |
| a novel antibiotic bone assay by liquid chromatography/tandem mass spectrometry for quantitation of tigecycline in rat bone. | tigecycline (tygacil) is a first-in-class, broad spectrum antibiotic with activity against antibiotic-resistant organisms. in rats and humans, tigecycline readily distributes to bone tissue but its accuracy of quantitation via liquid chromatography/mass spectrometry (lc/ms/ms) is hindered by a low extraction recovery when using a conventional plasma extraction method. to overcome this issue, we have identified an effective extraction solvent for quantitation of tigecycline in rat bone. the curre ... | 2007 | 17537611 |
| genomic diversity and relationship of bacillus thuringiensis and bacillus cereus by multi-rep-pcr fingerprinting. | the genomic diversity and relationship among 56 bacillus thuringiensis and bacillus cereus type strains were investigated by multi-rep-pcr fingerprinting consisting of three pcr reactions targeting the enterobacterial eric1 and eric2 and the streptococcal boxa1r consensus sequences. a total of 113 polymorphic bands were generated in the rep-pcr profiles that allowed tracing of a single dendrogram with three major groups. bacillus cereus strains clustered together in the a and b groups. most of t ... | 2007 | 17538643 |
| mycorrhizal fungi and ectomycorrhiza associated bacteria isolated from an industrial desert soil protect pine seedlings against cd(ii) impact. | effects of mycorrhization with amanita rubescens or hebeloma sinapizans and dual inoculation with the fungi and ectomycorrhiza associated bacteria (emab) pseudomonas putida or bacillus cereus on seedling growth and accumulation of cd(ii) in pinus sylvestris were studied. both fungal and bacterial species were isolated from roots of pines growing in an industrial area polluted with high concentrations of heavy metals. during mycorrhization, a. rubescens colonized higher number of pine seedlings t ... | 2007 | 17541824 |
| antibacterial activity of extract, fractions and four compounds extracted from piper solmsianum c. dc. var. solmsianum (piperaceae). | piper solmsianum c. dc. var. solmsianum (piperaceae) is a shrub commonly found in areas with wet tropical soils. other piper species have been used in folk medicine as antitumoral and antiseptic agents. we studied the crude methanolic extract, some organic fractions and compounds isolated from this plant for possible antimicrobial activity against gram-positive and gram-negative bacteria. the bioautographic assays disclosed three inhibition zones. the minimal inhibitory concentration (mic) and m ... | 2007 | 17542481 |
| synthesis, characterization and antimicrobial activity of new aliphatic sulfonamide. | a series of novel aliphatic sulfonamide derivatives (1-7) were synthesized and characterized by elemental analyses, ft-ir, (1)h nmr, (13)c nmr and lc-ms techniques. all the synthesized compounds were evaluated in vitro as antimicrobial agents against representative strains of gram-positive (staphylococcus aureus atcc 25953, bacillus cereus atcc 6633 and listeria monocytogenes atcc li6 (isolate), gram-negative bacteria (escherichia coli atcc 11230) and antifungal agent against candida albicans (c ... | 2007 | 17544281 |
| the contribution of gc1qr/p33 in infection and inflammation. | human gc1qr/p33 is a multi-compartmental and multi-functional cellular protein expressed on a wide range of tissues and cell types including lymphocytes, endothelial cells, dendritic cells, and platelets. although originally isolated as a receptor for c1q by virtue of its affinity (k(d)=15-50 nm), and specificity for the globular heads of this molecule, a large body of evidence has now been accumulated which shows that in addition to c1q, gc1qr can serve as a receptor for diverse proinflammatory ... | 2007 | 17544818 |
| quantitative analysis of population heterogeneity of the adaptive salt stress response and growth capacity of bacillus cereus atcc 14579. | bacterial populations can display heterogeneity with respect to both the adaptive stress response and growth capacity of individual cells. the growth dynamics of bacillus cereus atcc 14579 during mild and severe salt stress exposure were investigated for the population as a whole in liquid culture. to quantitatively assess the population heterogeneity of the stress response and growth capacity at a single-cell level, a direct imaging method was applied to monitor cells from the initial inoculum ... | 2007 | 17545319 |
| germination of bacillus cereus spores is induced by germinants from differentiated caco-2 cells, a human cell line mimicking the epithelial cells of the small intestine. | spores of 11 enterotoxigenic strains of bacillus cereus isolated from foods and humans adhered with similar efficiencies to caco-2 cells, whereas subsequent germination triggering was observed with only 8 of these strains. notably, hep-2 cells did not trigger germination, while spores of all strains displayed similar germination efficiencies in brain heart infusion broth. | 2007 | 17545321 |
| crystal structure of homoserine o-succinyltransferase from bacillus cereus at 2.4 a resolution. | | 2007 | 17546672 |
| influence of biofilm composition on the resistance to detachment. | bacillus cereus and pseudomonas fluorescens were used to develop monoculture biofilms in a bioreactor rotating system using a stainless steel cylinder for biofilm formation. the biofilms were allowed to grow for 7 days, exposed continuously to a reynolds number of agitation (rea) of 2,400. afterwards, the biofilms were characterised in terms of respiratory activity, amount of biomass, cellular density, cellular size and total and extracellular proteins and polysaccharides. the biofilm mechanical ... | 2007 | 17547019 |
| characterization of a small plcr-regulated gene co-expressed with cereolysin o. | in the human pathogen bacillus cereus, the expression of most extracellular virulence factors is controlled by the transcriptional activator plcr. among these virulence factors, cereolysin o (clo) is an haemolysin belonging to the cholesterol-dependant cytolysins, a protein family extensively studied in gram-positive bacteria. | 2007 | 17555563 |
| neutral sphingomyelinase-induced ceramide triggers germinal vesicle breakdown and oxidant-dependent apoptosis in xenopus laevis oocytes. | ceramide regulates many cellular processes, including cell growth, differentiation, and apoptosis. although the effects of exogenous bacterial neutral sphingomyelinase (smase) in xenopus laevis oocytes have been investigated, its microinjection into oocytes has not been reported previously. thus, we compared the incubation versus microinjection of the neutral bacillus cereus sphingomyelinase (bsmase) to examine whether the topology of ceramide generation determines its effects on the fate of ooc ... | 2007 | 17556754 |
| synthesis and antibacterial activity of tert-butyl [1-benzyl-2[(4-aryl-2-thiazolyl)hydrazono]ethyl]carbamate derivatives. | the increasing clinical importance of drug-resistant fungal and bacterial pathogens has lent additional urgency to microbiological research and new antibacterial compound development. for this purpose, new tert-butyl[1-benzyl-2[(4-aryl-2-thiazolyl)hydrazono]ethyl]carbamate derivatives were synthesized and evaluated for antibacterial activity. the reaction of boc-l-phenylalaninal with thiosemicarbazide gave the thiosemicarbazone which furnished the title compounds by reaction with phenacyl bromid ... | 2007 | 17562564 |
| antibacterial properties and major bioactive components of cinnamon stick (cinnamomum burmannii): activity against foodborne pathogenic bacteria. | cinnamomum burmannii blume (cinnamon stick) from indonesia is a little-investigated spice. in this study, the antibacterial activity, minimum inhibitory concentration (mic), and minimum bactericidal concentration (mbc) of cinnamon stick extract were evaluated against five common foodborne pathogenic bacteria (bacillus cereus, listeria monocytogenes, staphylococcus aureus, escherichia coli, and salmonella anatum). cinnamon stick extract exhibited significant antibacterial properties. major compou ... | 2007 | 17567030 |
| microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil. | in this study we evaluated the capacity of a defined microbial consortium (five bacteria: mycobacterium fortuitum, bacillus cereus, microbacterium sp., gordonia polyisoprenivorans, microbacteriaceae bacterium, naphthalene-utilizing bacterium; and a fungus identified as fusarium oxysporum) isolated from a pahs contaminated landfarm site to degrade and mineralize different concentrations (0, 250, 500 and 1000 mg kg(-1)) of anthracene, phenanthrene and pyrene in soil. pahs degradation and mineraliz ... | 2008 | 17572084 |
| the identification of antibacterial compounds for the development of enhanced media for the detection of foodborne fungi. | in an effort to identify a more suitable antibiotic for utilization in mycological media, 12 food borne fungal species from various genera including alternaria alternata, aspergillus flavus, a. niger, eurotium chevalieri, fusarium moniliforme, penicillium sp., rhizopus stolonifer, saccharomyces cerevisiae, candida tropicalis, geotrichum candidum, rhodotorula glutinis and kluyveromyces thermotolerans along with 21 chloramphenicol-resistant bacterial isolates from fresh produce and atcc cultures o ... | 2007 | 17574697 |
| flagellin (flic) protein sequence diversity among bacillus thuringiensis does not correlate with h serotype diversity. | in escherichia coli, the flic gene encodes flagellin, the protein responsible for eliciting the immunological reaction in h serotyping. here, the presence of the flagellin flic gene was studied in 86 bacillus thuringiensis strains encompassing 67 h serotypes. nineteen strains from four additional species in the b. cereus sensu lato group, b. cereus, b. anthracis, b. mycoides, and b. weihenstephanensis, were added for comparison purposes. the flic genes were amplified, cloned and their nucleotide ... | 2007 | 17578675 |
| minimizing the level of bacillus cereus spores in farm tank milk. | in a year-long survey on 24 dutch farms, bacillus cereus spore concentrations were measured in farm tank milk (ftm), feces, bedding material, mixed grass and corn silage, and soil from the pasture. the aim of this study was to determine, in practice, factors affecting the concentration of b. cereus spores in ftm throughout the year. in addition, the results of the survey were used in combination with a previously published modeling study to determine requirements for a strategy to control b. cer ... | 2007 | 17582113 |
| antimicrobial activities of indian berberis species. | the antimicrobial activity of hydroalcoholic extracts of four berberis species viz. berberis aristata, berberis asiatica, berberis chitria and berberis lycium were tested against eleven bacterial and eight fungal strains. b. aristata root extract gave low mics values against bacillus cereus, escherichia coli, staphylococcus aureus and aspergillus flavus while stem extract against b. cereus and streptococcus pneumoniae. | 2007 | 17583443 |
| postoperative endophthalmitis caused by bacillus cereus and chlamydia trachomatis. | to describe the clinical manifestations and outcomes in 4 patients with endophthalmitis caused by bacillus cereus and chlamydia trachomatis. | 2007 | 17586388 |
| susceptibility of bacillus anthracis, bacillus cereus, bacillus mycoides, bacillus pseudomycoides and bacillus thuringiensis to 24 antimicrobials using sensititre automated microbroth dilution and etest agar gradient diffusion methods. | to examine susceptibilities of bacillus anthracis and related species to 24 antimicrobials using and concurrently comparing two methods. | 2007 | 17586563 |
| the 2.2 a resolution crystal structure of bacillus cereus nif3-family protein yqfo reveals a conserved dimetal-binding motif and a regulatory domain. | yqfo of bacillus cereus is a member of the widespread nif3 family of proteins, which has been highlighted as an important target for structural genomics. the n- and c-terminal domains are conserved across the family and contain a dimetal-binding motif in a putative active site. yqfo contains an insert in the middle of the protein, present in a minority of bacterial family members. the structure of yqfo was determined at a resolution of 2.2 a and reveals conservation of the putative active site. ... | 2007 | 17586767 |
| bacterial, fungal and yeast contamination in six brands of irreversible hydrocolloid impression materials. | this study assessed the level of contamination of six commercially available irreversible hydrocolloids (two containing chlorhexidine) and identified the contamination present in the materials. petri dishes containing selective and enriched culture media were inoculated with alginate powder (0.06 g), in triplicate. after incubation (37 degrees c/7 days), the colony-forming units (cfu) were counted and gram stained. biochemical identification of the different morphotypes was also performed. the c ... | 2007 | 17589644 |
| epsilon-poly-l-lysine and nisin a act synergistically against gram-positive food-borne pathogens bacillus cereus and listeria monocytogenes. | to test epsilon-poly-l-lysine and nisin a for possible synergistic activity against selected food-borne pathogens and food spoilage organisms. | 2007 | 17594454 |
| microbial production of 4-hydroxybenzylidene acetone, the direct precursor of raspberry ketone. | to investigate the enzymatic aldol reaction between acetone as a donor and 4-hydroxybenzaldehyde as a receptor to generate 4-(4-hydroxyphenyl)-but-3-ene-2-one or 4-hydroxybenzylidene acetone, the direct precursor of 4-(4-hydroxyphenyl)-butan-2-one or raspberry ketone, using different species of filamentous fungi and bacteria. | 2007 | 17594457 |
| marine algae: screening for a potent antibacterial agent. | this study was done to investigate the antimicrobial potentiality of the marine algae collected from different coastal regions of gujarat and screened for the same. twenty-six marine algae belonging to rhodophyceae, chlorophyceae and phaeophyceae were screened for their potential antibacterial activity against five clinically important bacterial strains, namely bacillus cereus, micrococcus flavus, citrobacter freundii, klebsiella pneumoniae and pseudomonas testosterone. acetone and methanol were ... | 2007 | 17594989 |
| synthesis and antibacterial activity of bis[2-amino-4-phenyl-5-thiazolyl] disulfides. | different dimeric disulfides, having the basic skeleton of bis[2-amino-4-phenyl-5-thiazolyl] disulfides were synthesized in a straightforward manner from acetophenones. 2-amino-4-phenyl-1,3-thiazoles were prepared by the reaction of thiourea with substituted acetophenones in the presence of iodine which were then converted to the title compounds. all the compounds were subjected to preliminary evaluation for their biological activity against gram positive and gram negative bacteria. some of the ... | 2007 | 17603192 |
| prediction and assignment of function for a divergent n-succinyl amino acid racemase. | the protein databases contain many proteins with unknown function. a computational approach for predicting ligand specificity that requires only the sequence of the unknown protein would be valuable for directing experiment-based assignment of function. we focused on a family of unknown proteins in the mechanistically diverse enolase superfamily and used two approaches to assign function: (i) enzymatic assays using libraries of potential substrates, and (ii) in silico docking of the same librari ... | 2007 | 17603539 |
| survival, isolation and characterization of a psychrotrophic bacillus cereus strain from a mayonnaise-based ready-to-eat vegetable salad. | incidence and population levels of bacillus cereus in american salad, an industrially manufactured, packaged and refrigerated deli salad containing vegetables and mustard, were determined. of 12 ready-to-eat samples examined, one (8.3%) was positive for b. cereus at less than 5 x 10(3)cfu g(-1). according to the iso confirmation procedure, a strain was isolated and further characterized and identified as b. cereus epso-35as by api 50ch/20e phenotypic system, combined with additional tests of mot ... | 2007 | 17613363 |
| nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner. | to investigate the bactericidal activity of antiviral and anticancer nucleoside analogues against a variety of pathogenic bacteria and characterize the activating enzymes, deoxyribonucleoside kinases (dnks). | 2007 | 17615154 |
| purification and characterization of a solvent and detergent-stable novel protease from bacillus cereus. | a protease-producing bacterium was isolated from slaughterhouse waste samples, hyderabad, india. it was related to bacillus cereus on the basis of 16s rrna gene sequencing and biochemical properties. the protease was purified to homogeneity using ammonium sulfate precipitation, and ion exchange chromatography with a fold purification of 1.8 and a recovery of 49%. the enzyme had a relative molecular weight of 28kda, ph and temperature optima for this protease were 10 and 60 degrees c. the activit ... | 2009 | 17616381 |
| [open leg fracture with bacillus cereus infection]. | to investigate leg fractures infected with bacillus cereus in a traumatology-orthopedic ward of a university hospital. | 2007 | 17629655 |
| high resolution fesem and tem reveal bacterial spore attachment. | transmission electron microscopy (tem) studies in the 1960s and early 1970s using conventional thin section and freeze fracture methodologies revealed ultrastructural bacterial spore appendages. however, the limited technology at that time necessitated the time-consuming process of imaging serial sections and reconstructing each structure. consequently, the distribution and function of these appendages and their possible role in colonization or pathogenesis remained unknown. by combining high re ... | 2007 | 17637074 |
| walnut (juglans regia l.) leaves: phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. | different cultivars of walnut (juglans regia l.) leaves (cv. lara, franquette, mayette, marbot, mellanaise and parisienne) grown in portugal, were investigated in what concerns phenolic compounds and antimicrobial and antioxidant properties. phenolics analysis was performed by reversed-phase hplc/dad and 10 compounds were identified and quantified: 3- and 5-caffeoylquinic acids, 3- and 4-p-coumaroylquinic acids, p-coumaric acid, quercetin 3-galactoside, quercetin 3-pentoside derivative, querceti ... | 2007 | 17637491 |
| [valuation for usefulness of selected chromosomal markers for bacillus anthracis identification. i. valuation for markers sg-749, sg-450 and sg-300]. | the article presents results of valuation for b. anthracis-specificity and usefulness for its identification obtained for different chromosomal markers. in the first part of the study markers sg-749, sg-300 and sg-450 were analyzed. for the investigation rflp-pcr and msscp techniques were used and different electrophoresis methods were tested. the results gave an information not only about specificity of tested markers but also about the possibility of shorten time necessary to obtain results of ... | 2006 | 17642312 |
| screening and evaluation of probiotics as a biocontrol agent against pathogenic vibrios in marine aquaculture. | the present work aims at finding potential probionts from marine sources as a biocontrol agent against pathogenic vibrio species in shrimp larval culture. | 2007 | 17651222 |
| the initial surface composition and topography modulate sphingomyelinase-driven sphingomyelin to ceramide conversion in lipid monolayers. | changes of the initial composition and topography of mixed monolayers of sphingomyelin and ceramide modulate the degradation of sphingomyelin by bacillus cereus sphingomyelinase. the presence of initial lateral phase boundary due to coexisting condensed and expanded phase domains favors the precatalytic steps of the reaction. the amount and quality of the domain lateral interface, defined by the type of boundary undulation, appears as a modulatory supramolecular code which regulates the catalyti ... | 2007 | 17652769 |
| [second derivative ftir spectroscopic study of bacillus cereus]. | some particular cell components of intact bacillus cereus could be detected and identified by fourier transform infrared spectroscopy (ftir). ftir were collected directly, then the ftir spectra of the samples were converted into second derivative spectra. typical marker bands were used to identify these bacterial cell components such as capsules, endospores or storage materials. capsules were detected in cell by a strong amide band near 1654 cm(-1) typical for alpha-helical structures and by str ... | 2007 | 17655100 |
| a novel vector element providing multicopy vector integration in arxula adeninivorans. | an arxula adeninivorans vector element has been identified that provides multicopy integration in an atrp1 host strain. the element consists of the atrp1 selection marker fused to a newly generated truncated aleu2 promoter of 53 bp. in the described example eight copies of an amya expression vector encoding heterologous alpha-amylase from bacillus amyloliquefaciens are integrated in the genome of the recombinant strain instead of a single copy observed when using the atrp1 element with the compl ... | 2007 | 17655689 |
| flhf, a signal recognition particle-like gtpase, is involved in the regulation of flagellar arrangement, motility behaviour and protein secretion in bacillus cereus. | flagellar arrangement is a highly conserved feature within bacterial species. however, only a few genes regulating cell flagellation have been described in polar flagellate bacteria. this report demonstrates that the arrangement of flagella in the peritrichous flagellate bacillus cereus is controlled by flhf. disruption of flhf in b. cereus led to a reduction in the number of flagella from 10-12 to 1-3 filaments per cell in the insertion mutant mp06. moreover, compared to the parental strain, mp ... | 2007 | 17660418 |
| substrate analysis of homoserine acyltransferase from bacillus cereus. | substrate specificity within the family of enzymes designated as homoserine transsuccinylases is variable, with some organisms utilizing succinyl-coa and other organisms utilizing acetyl-coa. in this study it is shown that the enzyme from bacillus cereus uses acetyl-coa as its acyl donor, but its catalytic rate is significantly lower than other hts family members. bchts is inactivated by both iodoacetamide and diethyl pyrocarbonate and the enzyme can be partially protected from inactivation by t ... | 2007 | 17662245 |
| inhibition of bacterial cell division protein ftsz by cinnamaldehyde. | cinnamaldehyde is a natural product from spices that inhibits cell separation in bacillus cereus. cell division is regulated by ftsz, a prokaryotic homolog of tubulin. ftsz assembles into the z-ring at the site of cell division. here, we report the effect of cinnamaldehyde on ftsz and hence on the cell division apparatus. cinnamaldehyde decreases the in vitro assembly reaction and bundling of ftsz. it is found that cinnamaldehyde perturbs the z-ring morphology in vivo and reduces the frequency o ... | 2007 | 17662960 |
| biology and taxonomy of bacillus cereus, bacillus anthracis, and bacillus thuringiensis. | three species of the bacillus cereus group (bacillus cereus, bacillus anthracis, and bacillus thuringiensis) have a marked impact on human activity. bacillus cereus and b. anthracis are important pathogens of mammals, including humans, and b. thuringiensis is extensively used in the biological control of insects. the microbiological, biochemical, and genetic characteristics of these three species are reviewed, together with a discussion of several genomic studies conducted on strains of b. cereu ... | 2007 | 17668027 |
| characterization of bacillus and pseudomonas strains with suppressive traits isolated from tomato hydroponic-slow filtration unit. | bacillus and pseudomonas spp. are known to be involved in plant pathogenic fungi elimination during the slow filtration process used in tomato soilless cultures. we isolated 6-8 strains of both bacillus and pseudomonas from the top, middle, and bottom sections of filters and identified them after 16s rdna sequencing. four pseudomonas strains were identified as pseudomonas fulva, 5 as pseudomonas plecoglossicida, and 12 as pseudomonas putida. the use of specific oligonucleotide polymerase chain r ... | 2007 | 17668039 |
| risk factors for post-traumatic endophthalmitis in patients with positive intraocular cultures. | to determine the risk factors for post-traumatic endophthalmitis in patients with positive intraocular culture. | 2007 | 17671943 |
| [detection of some toxin genes related to pathogenicity in bacillus cereus group strains]. | bacillus thuringiensis is a member of the b. cereus group, which also contains b. cereus, b. mycoides, b. pseudomycoides , b. anthracis and b. weihenstephanensis. among them, b. thuringiensis and b. cereus share a high level chromosomal similarity and are phenotypically similar except that b. thuringiensis has insecticidal plasmid encoding crystal proteins. twenty-six b. cereus group strains were surveyed in this study for the presence of enterotoxin genes and other toxin genes related to pathog ... | 2007 | 17672293 |
| bacterial degradation and corrosion of naphtha in transporting pipeline. | five naphtha hydrocarbon-degrading bacteria including representative strains of the two classified species (serratia marcescensar1, bacillus pumilusar2, bacillus carboniphilus ar3, bacillus megaterium ar4, and bacillus cereus ar5) were identified by 16s rdna gene sequence in a naphtha-transporting pipeline. the naphtha-degrading strains were able to be involved in the corrosion process of api 5lx steel and also utilized the naphtha as the sole carbon source. the biodegradation of naphtha by the ... | 2007 | 17680305 |
| broad-spectrum antimicrobial activity of the reactive compounds generated in vitro by manduca sexta phenoloxidase. | although quinone production and melanin formation are widely recognized as an integral part of the insect defense system, experimental evidence is lacking that the proteolytic activation of prophenoloxidase participates in the direct killing of invading microbes-active phenoloxidase generates quinones that polymerize to form melanin. here, we report the antimicrobial effect of reactive intermediates produced in phenoloxidase-catalyzed reactions. after being treated with manduca sexta phenoloxida ... | 2007 | 17681234 |
| purification and identification of a novel leucine aminopeptidase from bacillus thuringiensis israelensis. | a novel leucine aminopeptidase was purified from a bacillus thuringiensis israelensis (bti) culture. the purification stages included heating the concentrated supernatant to 65 degrees c for 90 min, anion-exchange chromatography by deae cellulose, and hydrophobic chromatography by phenyl sepharose. the specific activity of leucine aminopeptidase after the hydrophobic chromatography increased by 215.5-fold and the yield was 16%. the molecular weight of the active enzyme was 59 kda. mass spectrome ... | 2007 | 17682820 |
| identification of emesis-causing bacillus cereus strains by polymerase chain reaction: preliminary results. | | 2007 | 17682912 |
| inhibitory effect of melastoma candidum d. don acetone extract on foodborne pathogenic bacteria survival in food products. | melastoma candidum d. don, a taiwanese folk medicinal plant, has high levels of antibacterial and bactericidal activity. our aim was to determine whether and to what extent an acetone extract of this plant inhibits the growth of foodborne pathogenic bacteria. m. candidum acetone extract had marked inhibitory effect on test bacteria introduced into sliced pork, which was then stored at 4 degrees c. at the end of storage (day 12), the bacterial concentrations dropped by 1.59 to 2.91 log cfu/g comp ... | 2007 | 17685331 |
| site-specific and asymmetric hydrolysis of prochiral 2-phenyl-1,3-propanediol diacetate by a bacterial esterase from an isolated strain. | bacillus cereus 809a and burkholderia sp. 711c were isolated from soil. these strains demonstrate hydrolysis activity towards prochiral 2-phenyl-1,3-propanediol diacetate and accumulated the corresponding chiral monoacetates into the reaction mixture. when 2-phenyl 1,3-propanediol diacetate was used as a substrate, the produced monoacetates with burkholderia sp. 711c were obtained in a racemic form but that produced by bacillus cereus 809a showed an excess of the (s)-form. the resting cell react ... | 2007 | 17690472 |
| synthesis, structure and antimicrobial activity of manganese(ii) and cobalt(ii) complexes of the polyether ionophore antibiotic sodium monensin a. | mononuclear neutral manganese(ii) and cobalt(ii) complexes with the antibiotic sodium monensin a (mon-na, 1b) were synthesized and characterized. the crystal structures of m(mon-na)2cl2.h2o (m=mn, 2; m=co, 3) were determined by x-ray crystallography. the complexes crystallize in monoclinic space group c2 with a tetrahedrally coordinated transition metal attached to oxygen atoms of deprotonated carboxyl groups of two sodium monensin molecules and two chloride ions. the sodium ion remains in the c ... | 2008 | 17692921 |
| synthesis and antimicrobial activity of some new 1,3,4-thiadiazole and 1,2,4-triazole compounds having a d,l-methionine moiety. | new 1,3,4-thiadiazole, 5a-e, and 1,2,4-triazolecompounds 6a-c, containing a d,l-methionine moiety were synthesized by intramolecular cyclization of 1,4-disubstituted thiosemicarbazides 4a-e in acid and alkaline media, respectively. the potential antimicrobial effects of the synthesized compounds were investigated using the staphylococcus aureus atcc 25923, bacillus antracis atcc 8705, bacillus cereus atcc 10987, sarcina lutea atcc 9341 and escherichia coli atcc 25922 strains. the newly synthesiz ... | 2007 | 17693957 |
| [usefulness of the thoracoscopic surgery under local anesthesia and irrigation for the patient with bacillus cereus empyema; report of a case]. | the case was 54-year-old male with some risks such as chronic heart failure, atrial fibrillation, and liver chirrhosis. he was admitted because of severe back pain and diagnosed as empyema by preoperative thoracentesis. by thoracoscopic procedures under local anesthesia, fibrinopurulent tissues were cleaned as much as possible and 3 of chest tubes were replaced. the final diagnosis was bacillus cereus pyothorax by bacterial cultures of pleural effusion. intrathoracic cavity was cleaned with phys ... | 2007 | 17703630 |
| control of microorganisms and reduction of biogenic amines in chicken breast and thigh by irradiation and organic acids. | the effect of irradiation or organic acid treatment of raw chicken breast and thigh meat to control inoculated microorganisms and the production of biogenic amines (ba) was studied. bacillus cereus, enterobacter cloacae, and alcaligenes faecalis were selected and inoculated into raw ground chicken breast and thigh meat at approximately 10(7) cfu/g. the samples were irradiated at 0, 0.5, 1, and 2 kgy or mixed with a 0.2 m solution of acetic, citric, or lactic acid (1 ml for 10 g of meat sample) f ... | 2007 | 17704395 |
| characterization of the adenine nucleoside specific phosphorylase of bacillus cereus. | adenosine phosphorylase, a purine nucleoside phosphorylase endowed with high specificity for adenine nucleosides, was purified 117-fold from vegetative forms of bacillus cereus. the purification procedure included ammonium sulphate fractionation, ph 4 treatment, ion exchange chromatography on deae-sephacel, gel filtration on sephacryl s-300 hr and affinity chromatography on n(6)-adenosyl agarose. the enzyme shows a good stability to both temperature and ph. it appears to be a homohexamer of 164+ ... | 2007 | 17707115 |
| evidence for a dinuclear active site in the metallo-beta-lactamase bcii with substoichiometric co(ii). a new model for metal uptake. | metallo-beta-lactamases are zinc-dependent enzymes that constitute one of the main resistance mechanisms to beta-lactam antibiotics. metallo-beta-lactamases have been characterized both in mono- and dimetallic forms. despite many studies, the role of each metal binding site in substrate binding and catalysis is still unclear. this is mostly due to the difficulties in assessing the metal content and site occupancy in solution. for this reason, co(ii) has been utilized as a useful probe of the act ... | 2007 | 17715135 |
| on the origin and fate of the fatty inclusions in a strain of bacillus cereus. | | 1945 | 17730624 |
| impact of the probiotic bacteria enterococcus faecium ncimb 10415 (sf68) and bacillus cereus var. toyoi ncimb 40112 on the development of serum igg and faecal iga of sows and their piglets. | to examine the influence of two different probiotic bacteria on the humoral immune system of swine, two animal studies were carried out with sows and their litters. the sows' feed was supplemented with either enterococcusfaecium ncimb 10415 (sf68) or bacillus cereus var. toyoi ncimb 40112 beginning early in pregnancy. the total iga content in the faeces as well as the total igg concentration in the blood of the sows was recorded before and after weaning. the same parameters were determined in th ... | 2007 | 17760301 |
| bacillus acidiceler sp. nov., isolated from a forensic specimen, containing bacillus anthracis px02 genes. | research at the center for biological defense identified plasmid-borne forms of bacillus anthracis pxo2 genes in a gram-positive, endospore-forming rod, isolated from a forensic specimen considered a credible threat of harbouring anthrax. conventional, commercial and molecular-based methods indicated that the isolate (cbd 119(t)) was not b. anthracis and considered not to be a member of the bacillus cereus group. based on the 16s rrna gene sequence similarities, strain cbd 119(t) was most closel ... | 2007 | 17766868 |
| a systematic review of the clinical, public health and cost-effectiveness of rapid diagnostic tests for the detection and identification of bacterial intestinal pathogens in faeces and food. | to determine the diagnostic accuracy of tests for the rapid diagnosis of bacterial food poisoning in clinical and public health practice and to estimate the cost-effectiveness of these assays in a hypothetical population in order to inform policy on the use of these tests. | 2007 | 17803865 |
| maintenance of sterility in 1-ml polypropylene syringes. | the sterility of syringes filled with a growth-promoting broth when stored under various temperature conditions was studied. | 2007 | 17823109 |
| ethnobotanical survey and antibacterial activity of some plants used in guinean traditional medicine. | a total of 418 healers have been interviewed in guinea, a coastal country of west africa, ranging between 7 degrees 30 and 12 degrees 30 of northern latitude and 8 degrees and 15 degrees of western longitude. plant species used by the local inhabitants to treat infectious diseases were identified using ethnobotanical, ethnographic and taxonomic methods. during these investigations, 218 plants were registered, of which the following were the most frequently used: erythrina senegalensis, bridelia ... | 2007 | 17825510 |
| synthesis, spectroscopic and biological aspects of iron(ii) complexes. | five novel coordinated complexes of iron(ii) with ciprofloxacin and neutral bidentate ligands have been prepared and characterized using elemental analyses, magnetic measurements, ir spectra, uv-vis spectral, thermogravimetric analyses, 1h-nmr and 13c-nmr. the antimicrobial activity of the individual ligands, metal salt and metal complexes with respect to bacillus subtilis, escherichia coli, bacillus cereus, staphylococcus aureus, salmonella typhi, serratia marcescens, aspergillus niger, aspergi ... | 2007 | 17847716 |
| anaerobic biodegradation of fluoranthene under methanogenic conditions in presence of surface-active compounds. | bacillus cereus isolated from municipal wastewater treatment plant was used as a model strain to assess the efficiency of two anionic surfactants, a chemical surfactant and a biosurfactant during fluoranthene biodegradation under anaerobic methanogenic conditions. the surfactants selected for the study were linear alkyl benzene sulphonates (las) and rhamnolipid-biosurfactant complex from pseudomonas sp. ps-17. biodegradation of fluoranthene was monitored by gc/ms for a period up to 12th day. no ... | 2008 | 17869417 |
| influence of the probiotic bacillus cereus var. toyoi on the intestinal immunity of piglets. | in a feeding trial, sows and piglets were fed with the probiotic bacterium bacillus cereus var. toyoi as a feed additive, and the effects on immune cell populations were examined. the development of the gut immune system was determined for piglets at the ages of 14, 28, 35 and 56 days post partum. tissue samples of the jejunum and the continuous peyer's patch were used for enumeration of intraepithelial lymphocyte populations by fluorescence activated flow cytometry and fluorescence microscopy. ... | 2007 | 17870185 |
| food-poisoning and commercial air travel. | with the introduction of budget airlines and greater competitiveness amongst all airlines, air travel has now become an extremely popular form of travel, presenting its own unique set of risks from food poisoning. foodborne illness associated with air travel is quite uncommon in the modern era. however, when it occurs, it may have serious implications for passengers and when crew are affected, has the potential to threaten safety. quality, safe, in-flight catering relies on high standards of foo ... | 2007 | 17870632 |
| structure and biological activities of beta toxin from staphylococcus aureus. | beta toxin is a neutral sphingomyelinase secreted by certain strains of staphylococcus aureus. this virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. the structure of beta toxin was determined at 2.4-a resolution using crystals that were merohedrally twinned. this structure is similar to that of the sphingomyelinases of listeria ivanovii and bacillus cereus. beta toxin belongs to the dnase i folding superfamily; in addition to sphingomyel ... | 2007 | 17873030 |
| phenolic compounds and antimicrobial activity of olive (olea europaea l. cv. cobrançosa) leaves. | we report the determination of phenolic compounds in olive leaves by reversed-phase hplc/dad, and the evaluation of their in vitro activity against several microorganisms that may be causal agents of human intestinal and respiratory tract infections, namely gram positive (bacillus cereus, b. subtilis and staphylococcus aureus), gram negative bacteria (pseudomonas aeruginosa, escherichia coli and klebsiella pneumoniae) and fungi (candida albicans and cryptococcus neoformans). seven phenolic compo ... | 2007 | 17873849 |
| incidence and characterization of diarrheal enterotoxins of fecal bacillus cereus isolates associated with diarrhea. | a total of 490 stool specimens were collected from patients with diarrhea and healthy controls without diarrhea to investigate the incidence of bacillus cereus and its enterotoxins. b. cereus was found more significant in stools of persons with diarrhea than without diarrhea (9.5% versus 1.8%, p < 0.05), and was also detected more frequent but not significant in individuals aged > or =1 year and in adults than in children aged <1 year (11% and 8% versus 7.8%, p > 0.05). the hemolytic enterotoxin ... | 2007 | 17878069 |
| construction, crystal structure and application of a recombinant protein that lacks the collagen-like region of bcla from bacillus anthracis spores. | spores of bacillus anthracis, the causative agent of anthrax, are enclosed by an exosporium, which consists of a basal layer surrounded by a nap of hair-like filaments. the major structural component of the filaments is called bcla, which comprises a central collagen-like region (clr) and a globular c-terminal domain. here, the entire clr coding sequence of bcla was removed, and the resulting protein (tbcla) produced in escherichia coli. the crystallographic structure of tbcla was determined to ... | 2008 | 17879302 |
| differential analysis of bacillus anthracis after px01 plasmid curing and comprehensive data on bacillus anthracis infection in macrophages and glial cells. | bacillus anthracis is a gram-positive bacterial organism responsible for anthrax. this organism has two pathogenic plasmids: px01 and px02. the genetic function of px01, which comprises about 198 kb, is not known, except for a region called the pathogenic island, which contains three genes-pag, lef, and cya-that code for three toxic proteins. a 2-d difference gel electrophoresis (2-d dige) system was used to verify the existence of proteins controlled by the px01 plasmid, and protein regulation ... | 2007 | 17880004 |
| development of flexible antimicrobial films using essential oils as active agents. | the antimicrobial activity in the vapor-phase of laboratory-made flexible films of polypropylene (pp) and polyethylene/ethylene vinyl alcohol copolymer (pe/evoh) incorporating essential oil of cinnamon ( cinnamomum zeylanicum), oregano ( origanum vulgare), clove ( syzygium aromaticum), or cinnamon fortified with cinnamaldehyde was evaluated against a wide range of microorganisms: the gram-negative bacteria escherichia coli, yersinia enterocolitica, pseudomonas aeruginosa, and salmonella cholerae ... | 2007 | 17880148 |
| enhanced secondary metabolite (tanshinone) production of salvia miltiorrhiza hairy roots in a novel root-bacteria coculture process. | salvia miltiorrhiza bunge (lamiaceae) hairy root cultures were inoculated (at 0.02 and 0.2% v/v) and co-cultured with bacillus cereus bacteria. the root biomass growth was inhibited significantly by the bacteria inoculated to the root culture on the first day (day 0) but not by the bacteria inoculated on days 14 or 21 (in a 28-day overall period). on the other hand, the growth of the bacteria in the hairy root culture was also strongly inhibited by the hairy roots, partially because of the antib ... | 2007 | 17882415 |
| influence of an oil soluble inhibitor on microbiologically influenced corrosion in a diesel transporting pipeline. | microbial degradation of the oil soluble corrosion inhibitor (osci) baker nc 351 contributed to a decrease in inhibitor efficiency. corrosion inhibition efficiency was studied by the rotating cage and flow loop methods. the nature of the biodegradation of the corrosion inhibitor was also analysed using fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy and gas chromatography-mass spectrometry. the influence of bacterial activity on the degradation of the corrosion i ... | 2007 | 17882627 |
| [influence of bacillus cereus on microbiocenosis of gastrointestinal tract in rats]. | the modifying effect of bacillus cereus on intestinal microbiocenosis was investigated in eubiotic and disbiotic female rats. qualitative and quantitative characteristics of gut and mucosal microflora from different parts of rats' intestine were studied before and after intragastral application of b. cereus suspension. the single application of b. cereus suspension resulted in appearance of this bacterium in feces and in parietal mucin from all parts of the intestine. in eubiotic rats compared w ... | 2007 | 17882837 |
| microbial population present in fermented beverage 'cauim' produced by brazilian amerindians. | the tapirapé amerindians of the tapi'itãwa tribe produce several fermented foods and beverages among them the beverage called 'cauim'. this beverage is the main staple food for infants until two years old and their parents. for producing the beverage, several substrates are used, such as: cassava, rice, corn, maize and peanuts. the fermentation using mainly cassava was accomplished and samples were collected for chemical and microbiological analysis. a progressive acidification during the fermen ... | 2007 | 17888538 |
| enzymatic reduction of azo and indigoid compounds. | a customer- and environment-friendly method for the decolorization azo dyes was developed. azoreductases could be used both to bleach hair dyed with azo dyes and to reduce dyes in vat dyeing of textiles. a new reduced nicotinamide adenine dinucleotide-dependent azoreductase of bacillus cereus, which showed high potential for reduction of these dyes, was purified using a combination of ammonium sulfate precipitation and chromatography and had a molecular mass of 21.5 kda. the optimum ph of the az ... | 2007 | 17891390 |
| the exosporium of b. cereus contains a binding site for gc1qr/p33: implication in spore attachment and/or entry. | b. cereus, is a member of a genus of aerobic, gram-positive, spore-forming rod-like bacilli, which includes the deadly, b. anthracis. preliminary experiments have shown that gc1qr binds to b. cereus spores that have been attached to microtiter plates. the present studies were therefore undertaken, to examine if cell surface gc1qr plays a role in b. cereus spore attachment and/or entry. monolayers of human colon carcinoma (caco-2) and lung cells were grown to confluency on 6 mm coverslips in shel ... | 2007 | 17892212 |
| assembly of pili on the surface of bacillus cereus vegetative cells. | vegetative forms of bacillus cereus are reported to form pili, thin protein filaments that protrude up to 1 mum from the bacterial surface. pili are assembled from two precursor proteins, bcpa and bcpb, in a manner requiring a pilus-associated sortase enzyme (srtd). pili are also formed on the surface of bacillus anthracis expressing bcpa-srtd-bcpb. bcpa is distributed throughout the entire pilus, whereas bcpb appears positioned at its tip. in agreement with the hypothesis for pilus assembly in ... | 2007 | 17897374 |
| molecular and biochemical detection of fengycin- and bacillomycin d-producing bacillus spp., antagonistic to fungal pathogens of canola and wheat. | bacillus species are well known for their ability to control plant diseases through various mechanisms, including the production of secondary metabolites. bacillus subtilis dfh08, an antagonist of fusarium graminearum, and other bacillus spp. that are antagonists of common fungal pathogens of canola were screened for peptide synthetase biosynthetic genes of fengycin and bacillomycin d. specific polymerase chain reaction (pcr) primers identified b. subtilis strains dfh08 and 49 for the presence o ... | 2007 | 17898845 |
| molecular characterization of antimicrobial compound produced by lactobacillus acidophilus aa11. | approximately 63 strains of lactobacillus acidophilus were isolated from egyptian home-made cheese and examined for production of antagonism. only eight strains demonstrated inhibitory activity against spoilage microorganisms (i.e. staphylococcus aureus and bacillus cereus) and pathogens (i.e. e. coli, salmonella sp. and shigella sp.). lactobacillus acidophilus aa11 produced higher antimicrobial activity with a wide range of inhibition. the agent aa11 was sensitive to proteolytic enzymes and ret ... | 2007 | 17899791 |
| effectiveness of trisodium phosphate, acidified sodium chlorite, citric acid, and peroxyacids against pathogenic bacteria on poultry during refrigerated storage. | the effects of dipping treatments (15 min) in potable water or in solutions (wt/vol) of 12% trisodium phosphate (tsp), 1,200 ppm acidified sodium chlorite (asc), 2% citric acid (ca), and 220 ppm peroxyacids (pa) on inoculated pathogenic bacteria (listeria monocytogenes, staphylococcus aureus, bacillus cereus, salmonella enteritidis, escherichia coli, and yersinia enterocolitica) and skin ph were investigated throughout storage of chicken legs (days 0, 1, 3, and 5) at 3 +/- 1 degrees c. all chemi ... | 2007 | 17900083 |