| influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro. | objectives: the aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (ti) substrates on initial oral biofilm formation. materials and methods: nine bacterial species belonging to the normal oral microbiota, including: aggregatibacter actinomycetemcomitans, actinomyces israelii, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, parvimonas micra, porphyromonas gingivalis, prevotella intermedia, and streptococcus sanguinis were teste ... | 2011 | 21492236 |
| antigenicity of primary endodontic infection against macrophages by the levels of pge(2) production. | root canal contents are potent stimuli for proinflammatory cytokines involved in apical periodontitis. this study investigated target gram-negative bacterial species and endotoxins in primary endodontic infection with apical periodontitis, determined their antigenicity against macrophages through the levels of pge(2), and evaluated their relationship with clinical findings. | 2011 | 21496656 |
| tlr4 signaling via myd88 and trif differentially shape the cd4+ t cell response to porphyromonas gingivalis hemagglutinin b. | recombinant hemagglutinin b (rhagb), a virulence factor of the periodontal pathogen porphyromonas gingivalis, has been shown to induce protective immunity against bacterial infection. furthermore, we have demonstrated that rhagb is a tlr4 agonist for dendritic cells. however, it is not known how rhagb dendritic cell stimulation affects the activation and differentiation of t cells. therefore, we undertook the present study to examine the role of tlr4 signaling in shaping the cd4(+) t cell respon ... | 2011 | 21498664 |
| gingival tissue transcriptomes in experimental gingivitis. | jönsson d, ramberg p, demmer rt, kebschull m, dahlén g, papapanou pn. gingival tissue transcriptomes in experimental gingivitis. j clin periodontol 2011; doi: 10.1111/j.1600-051x.2011.01719.x. abstract: aims: we investigated the sequential gene expression in the gingiva during the induction and resolution of experimental gingivitis. material and methods: twenty periodontally and systemically healthy non-smoking volunteers participated in a 3-week experimental gingivitis protocol, followed by deb ... | 2011 | 21501207 |
| sialidase and sialoglycoproteases can modulate virulence in porphyromonas gingivalis. | the porphyromonas gingivalis recombinant vima can interact with the gingipains and several other proteins, including a sialidase. sialylation can be involved in protein maturation however its role in virulence regulation in p. gingivalis is unknown. the three sialidase related proteins in p. gingivalis showed the characteristic sialidase asp signature motif (sxdxgxtw) and other unique domains. to evaluate the roles of these genes, randomly chosen p. gingivalis isogenic mutants created by allelic ... | 2011 | 21502589 |
| sitafloxacin: in bacterial infections. | sitafloxacin is a fluoroquinolone antibacterial with in vitro activity against a broad range of gram-positive and -negative bacteria, including anaerobic bacteria, as well as against atypical pathogens. it is approved in japan for use in a number of bacterial infections caused by sitafloxacin-susceptible strains of staphylococcus spp., streptococcus pneumoniae, other streptococcus spp., enterococcus spp., moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., enterobacter sp ... | 2011 | 21504249 |
| viral-bacterial associations in acute apical abscesses. | objective: viral-bacterial and bacterial synergism have been suggested to contribute to the pathogenesis of several human diseases. this study sought to investigate the possible associations between 9 candidate endodontic bacterial pathogens and 9 human viruses in samples from acute apical abscesses. study design: dna extracts from purulent exudate aspirates of 33 cases of acute apical abscess were surveyed for the presence of 9 selected bacterial species using a 16s ribosomal rna gene-based nes ... | 2011 | 21507684 |
| detection and clonal analysis of anaerobic bacteria associated to endodontic-periodontal lesions. | background: microbial agents in the root canal systems can induce periodontal inflammation. the aims of this study were to detect anaerobic microorganims in endodontic-periodontal lesions, determine the genetic diversity among them and assess the simultaneous colonization of the pulp and periodontal microenvironments by a single clone. methods: twenty-seven teeth of patients with endodontic-periodontal lesions were selected. samples were spread on an agar-blood medium, the detection of each spec ... | 2011 | 21513472 |
| porphyromonas gingivalis is associated with protease-activated receptor-2 up-regulation in chronic periodontitis. | background: we previously reported a higher expression of protease-activated receptor-2 (par(2)) together with higher interleukin (il)1α, il-6, il-8, and tumor necrosis factor-alpha (tnf-α) levels, total proteolytic activity, porphyromonas gingivalis (p.gingivalis) prevalence, and neutrophil-protease 3 rna messenger (mrna) expression in subjects with chronic periodontitis compared to healthy controls. the aim of the present study is to expand this observation by considering the site level accord ... | 2011 | 21513479 |
| rheumatoid arthritis and periodontitis; a possible link via citrullination. | rheumatoid arthritis (ra) and chronic and aggressive periodontitis are chronic inflammatory disorders characterized by deregulation of the host inflammatory response. increased secretion of pro-inflammatory mediators results in soft and hard tissue destruction of the synovium and periodontium respectively. both diseases share risk factors and have pathological pathways in common, resulting in loss of function and disability as a final clinical outcome. this article discusses possible interaction ... | 2011 | 21515392 |
| inhibition of host- and bacteria-derived proteinases by natural anthocyanins. | santos j, la vd, bergeron c, grenier d. inhibition of host- and bacteria-derived proteinases by natural anthocyanins. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01372.x. © 2011 john wiley & sons a/s background and objectives: host- and bacteria-derived proteinases are considered to play critical roles in periodontitis progression. this study investigated the ability of a blackcurrant extract and its major anthocyanins (cyanidin-3-o-glucoside, cyanidin-3-o-rutinoside and delphinidin-3-o ... | 2011 | 21517858 |
| a member of the peptidase m48 superfamily of porphyromonas gingivalis is associated with virulence in vitro and in vivo. | in vivo-induced antigen technology was previously used to identify 115 genes induced in porphyromonas gingivalis w83 during human infection. one of these, pg2197, a conserved hypothetical protein which has homology to a zn-dependent protease, was examined with respect to a role in disease. | 2009 | 21523206 |
| tracking of plasma antibodies against aggregatibacter actinomycetemcomitans and porphyromonas gingivalis during 15 years. | plasma antibody measurements of antibody levels to periodontal pathogens may be used to support diagnosis, disease activity, classification, and prognosis of periodontitis. | 2009 | 21523211 |
| bacterial diversity in persistent periapical lesions on root-filled teeth. | the purpose of this study was to analyze the bacterial diversity in persistent apical lesions on root-filled teeth by using culture-independent molecular methods. | 2009 | 21523213 |
| rheumatoid arthritis and the role of oral bacteria. | rheumatoid arthritis (ra) and periodontal disease (pd) have shown similar physiopathologic mechanisms such as chronic inflammation with adjacent bone resorption in an immunogenetically susceptible host; however, pd has a well-recognized bacterial etiology while the cause of ra is unclear. some reports have indicated that an infectious agent in a susceptible host could be one possible trigger factor for ra, and it has been suggested that oral microorganisms, specialty periodontal bacteria could b ... | 2010 | 21523217 |
| porphyromonas gingivalis-dendritic cell interactions: consequences for coronary artery disease. | an estimated 80 million us adults have one or more types of cardiovascular diseases. atherosclerosis is the single most important contributor to cardiovascular diseases; however, only 50% of atherosclerosis patients have currently identified risk factors. chronic periodontitis, a common inflammatory disease, is linked to an increased cardiovascular risk. dendritic cells (dcs) are potent antigen presenting cells that infiltrate arterial walls and may destabilize atherosclerotic plaques in cardiov ... | 2010 | 21523219 |
| effect of the probiotic lactobacilli reuteri (prodentis) in the management of periodontal disease: a preliminary randomized clinical trial. | the aim of this study was to evaluate the effects of lactobacilli reuteri (prodentis) alone and in combination with scaling and root planing (srp) in a double blind, randomized, placebo-controlled clinical trial of volunteers with chronic periodontitis. | 2010 | 21523225 |
| analysis of microbiota associated with peri-implantitis using 16s rrna gene clone library. | peri-implantitis (pi) is an inflammatory disease which leads to the destruction of soft and hard tissues around osseointegrated implants. the subgingival microbiota appears to be responsible for peri-implant lesions and although the complexity of the microbiota has been reported in pi, the microbiota responsible for pi has not been identified. | 2010 | 21523229 |
| differential effects of periopathogens on host protease inhibitors slpi, elafin, scca1, and scca2. | secretory leukocyte peptidase inhibitors (slpi), elafin, squamous cell carcinoma antigen 1 and 2 (scca1 and scca2) are specific endogenous serine protease inhibitors expressed by epithelial cells that prevent tissue damage from excessive proteolytic enzyme activity due to inflammation. to determine the effects of various periopathogens on these protease inhibitors, we utilized human gingival epithelial cells (gecs) challenged with cell-free bacteria supernatants of various periopathogens porphyr ... | 2010 | 21523231 |
| spiramycin resistance in human periodontitis microbiota. | purpose: the occurrence of in vitro resistance to therapeutic concentrations of spiramycin, amoxicillin, and metronidazole was determined for putative periodontal pathogens isolated in the united states. materials and methods: subgingival plaque specimens from 37 consecutive adults with untreated severe periodontitis were anaerobically cultured, and isolated putative periodontal pathogens were identified to a species level. in vitro resistance to spiramycin at 4 μg/ml, amoxicillin at 8 μg/ml, an ... | 2011 | 21524712 |
| specific in situ visualization of plasma cells producing antibodies against porphyromonas gingivalis in gingival radicular cyst: application of the enzyme-labeled antigen method. | the enzyme-labeled antigen method was applied to visualize plasma cells producing antibodies to porphyromonas gingivalis, flora of the human oral cavity. antibodies to p. gingivalis have reportedly been detected in sera of patients with periodontitis. biotinylated bacterial antigens, ag53, and four gingipain domains (arg-pro, arg-hgp, lys-pro, and lys-hgp) were prepared by the cell-free protein synthesis system using the wheat germ extract. in paraformaldehyde-fixed frozen sections of rat lymph ... | 2011 | 21525188 |
| genetic transformation of an obligate anaerobe, p. gingivalis for fmn-green fluorescent protein expression in studying host-microbe interaction. | the recent introduction of "oxygen-independent" flavin mononucleotide (fmn)-based fluorescent proteins (fbfps) is of major interest to both eukaryotic and prokaryotic microbial biologists. accordingly, we demonstrate for the first time that an obligate anaerobe, the successful opportunistic pathogen of the oral cavity, porphyromonas gingivalis, can be genetically engineered for expression of the non-toxic green fbfp. the resulting transformants are functional for studying dynamic bacterial proce ... | 2011 | 21525983 |
| characterisation of the porphyromonas gingivalis conjugative transposon ctnpg1: determination of the integration site and the genes essential for conjugal transfer. | in our previous study, extensive genomic rearrangements were found in two strains of the gram-negative anaerobic bacterium porphyromonas gingivalis, and most of these rearrangements were associated with mobile genetic elements such as insertion sequences and conjugative transposons (ctns) (m. naito et al., dna res. 15: 215-225, 2008). ctnpg1, identified in p. gingivalis strain atcc 33277, was the first complete ctn reported for the genus porphyromonas. in the present study, we found that ctnpg1 ... | 2011 | 21527470 |
| periodontal conditions and prevalence of putative periodontopathogens and candida spp. in insulin-dependent type 2 diabetic and non-diabetic patients with chronic periodontitis-a pilot study. | objectives: the aims of this study were to evaluate periodontal conditions and identify the presence of aggregatibacter actinomycetemcomitans, porphyromonas gingivalis and tannerella forsythia, and four different species of candida (c. albicans, c. dubliniensis, c. glabrata and c. tropicalis) in periodontal pockets and furcation sites of insulin-dependent type 2 diabetic and non-diabetic patients with generalised chronic periodontitis. design: clinical parameters, including oral status assessed ... | 2011 | 21529777 |
| can oral pathogens influence allergic disease? | the hygiene hypothesis contends that fewer opportunities for infections and microbial exposures have resulted in more widespread asthma and atopic disease. consistent with that hypothesis, decreases in infectious oral diseases over the past half century have coincided with increases in the prevalence of asthma and other allergic diseases. this observation has led some researchers to speculate that exposures to oral bacteria, including pathogens associated with periodontal diseases, such as gingi ... | 2011 | 21531254 |
| dendritic cells, antibodies reactive with oxldl, and inflammation. | periodontitis appears to promote chronic inflammatory diseases, including atherosclerosis, but relevant mechanisms need clarification. oral bacteria induce antibodies that bind not only bacteria, but also oxldl. porphyromonas gingivalis and aggregatibacter actinomycetemcomitans induce remarkable igg responses that are dominated by igg2, and igg2 is ifn-γ-dependent and is promoted by dendritic cells (dcs). ldl-reactive antibodies induced by p. gingivalis and a. actinomycetemcomitans include anti- ... | 2011 | 21531918 |
| infection and rheumatoid arthritis: still an open question. | this review focuses on recent research that explores the role of infectious organisms in the development of autoimmunity and rheumatoid arthritis (ra). | 2011 | 21532483 |
| porphyromonas gingivalis participates in pathogenesis of human abdominal aortic aneurysm by neutrophil activation. proof of concept in rats. | abdominal aortic aneurysms (aaas) represent a particular form of atherothrombosis where neutrophil proteolytic activity plays a major role. we postulated that neutrophil recruitment and activation participating in aaa growth may originate in part from repeated episodes of periodontal bacteremia. | 2011 | 21533243 |
| porphyromonas gingivalis induction of microrna-203 expression controls suppressor of cytokine signaling 3 in gingival epithelial cells. | porphyromonas gingivalis is a pathogen in severe periodontal disease. able to exploit an intracellular lifestyle within primary gingival epithelial cells (gecs), a reservoir of p. gingivalis can persist within the gingival epithelia. this process is facilitated by manipulation of the host cell signal transduction cascades which can impact cell cycle, cell death and cytokine responses. using microarrays we investigated the ability of p. gingivalis 33277 to regulate microrna (mirna) expression in ... | 2011 | 21536793 |
| periodontal ligament and gingival fibroblasts participate in the production of tgf-β, interleukin (il)-8 and il-10. | the aim of this study was to quantify and compare the production of transforming growth factor beta (tgf-β), interleukin (il)-8 and il-10 by human cultured periodontal ligament and gingival fibroblasts both obtained from the same donors challenged with lipopolysaccharide (lps) from porphyromonas gingivalis. fibroblasts were exposed to 0.1-10 µg/ml of lps from p. gingivalis and after 24 h the supernatants were collected and analyzed by enzyme-linked immunosorbent assay (elisa). tgf-β protein prod ... | 2011 | 21537641 |
| inhibitory effect of lactobacillus reuteri on periodontopathic and cariogenic bacteria. | the interaction between lactobacillus reuteri, a probiotic bacterium, and oral pathogenic bacteria have not been studied adequately. this study examined the effects of l. reuteri on the proliferation of periodontopathic bacteria including aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, and tannerella forsythia, and on the formation of streptococcus mutans biofilms. human-derived l. reuteri strains (kctc 3594 and kctc 3678) and rat-derived l. reuteri kctc ... | 2011 | 21538238 |
| development of porphyromonas gingivalis-specific quantitative real-time pcr primers based on the nucleotide sequence of rpob. | species-specific quantitative real-time pcr (qpcr) primers were developed for the detection of porphyromonas gingivalis. these primers, pg-f/pg-r, were designed based on the nucleotide sequences of rna polymerase β-subunit gene (rpob). species-specific amplicons were obtained from the tested p. gingivalis strains but not in any of the other strains (46 strains of 46 species). the qpcr primers could detect as little as 4 fg of p. gingivalis chromosomal dna. these findings suggest that these qpcr ... | 2011 | 21538257 |
| diversity and quantitative analysis of archaea in aggressive periodontitis and periodontally healthy subjects. | matarazzo f, ribeiro ac, feres m, faveri m, mayer mpa. diversity and quantitative analysis of archaea in aggressive periodontitis and periodontally healthy subjects. j clin peridontol 2011; doi: 10.1111/j.1600-051x.2011.01734.x. abstract: aim: to investigate the diversity, levels and proportions of archaea in the subgingival biofilm of generalized aggressive periodontitis (gagp; n=30) and periodontally healthy (ph; n=30) subjects. materials and methods: diversity was determined by sequencing arc ... | 2011 | 21539593 |
| diverse effects of porphyromonas gingivalis on human osteoclast formation. | porphyromonas gingivalis is associated with periodontitis, a chronic inflammatory disease of the tooth-supporting tissues. a major clinical symptom is alveolar bone loss due to excessive resorption by osteoclasts. p. gingivalis may influence osteoclast formation in diverse ways; by interacting directly with osteoclast precursors that likely originate from peripheral blood, or indirectly by activating gingival fibroblasts, cells that can support osteoclast formation. in the present study we inves ... | 2011 | 21539907 |
| genomic comparison of invasive and rare non-invasive strains reveals porphyromonas gingivalis genetic polymorphisms. | porphyromonas gingivalis strains are shown to invade human cells in vitro with different invasion efficiencies, varying by up to three orders of magnitude. | 2011 | 21541093 |
| effect of periodontopathogen lipopolysaccharides and proinflammatory cytokines on cd46, cd55, and cd59 gene/protein expression by oral epithelial cells. | membrane-anchored complement regulatory proteins (crps), including cd46, cd55, and cd59, protect host cells from complement attack. in the present study, we investigated whether periodontopathogen lipopolysaccharide and proinflammatory cytokines modulate crp gene/protein expression in human oral epithelial cells. the lipopolysaccharide of treponema denticola and tannerella forsythia were the most potent for increasing the gene expression of cd55 and cd59, and to a lesser extent cd46, after a 48- ... | 2011 | 21545652 |
| porphyromonas gingivalis muty is involved in the repair of oxidative stress-induced dna mispairing. | the ability for dna mismatch repair, after oxidative stress-induced dna damage, is critical for the persistence of porphyromonas gingivalis in the inflammatory environment of the periodontal pocket. our previous report demonstrated that, in contrast to other organisms, the repair of oxidative stress-induced dna damage involving 8-oxo-7,8-dihydroguanine (8-oxog) may occur by a yet-to-be described mechanism in p. gingivalis. 8-oxog does not block dna replication; rather, it mispairs with adenine, ... | 2011 | 21545695 |
| interaction of oral bacteria with gingival epithelial cell multilayers. | primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. multilayers maintained at an air-liquid interface in low-calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. multilayers were infected with fluorescently labeled porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, fusobacterium nucleatum or streptococcus gord ... | 2011 | 21545698 |
| garlic allicin as a potential agent for controlling oral pathogens. | abstract garlic has been used medicinally throughout human history. allicin is considered the most therapeutic constituent of garlic. this study tested the antimicrobial activity of garlic allicin on oral pathogens associated with dental caries and periodontitis. allicin was found effective against all the tested bacteria. the broth dilution method revealed that planktonic growth of the cariogenic, gram-positive species streptococcus mutans, s. sobrinus, and actinomyces oris was inhibited by an ... | 2011 | 21548800 |
| increased expression of c-reactive protein gene in inflamed gingival tissues could be derived from endothelial cells stimulated with interleukin-6. | background: epidemiological studies have suggested periodontitis as a risk factor for ischemic heart disease. high sensitive c-reactive protein (hs-crp), a predictor of cardiovascular risk, is elevated in periodontitis patients. therefore, local infection-induced elevation of systemic crp could account for the relationship between the 2 diseases. however, the underlying mechanism of crp production in the periodontal tissues has not been fully elucidated. therefore, the aim of the present study w ... | 2011 | 21550586 |
| identification of mono- or poly-specific monoclonal antibody to porphyromonas gingivalis heat-shock protein 60. | the aim of this study was to define the immunoreactive specificity of porphyromonas gingivalis (p. gingivalis) heat shock protein (hsp) 60 in periodontitis and atherosclerosis. | 2011 | 21556254 |
| relationship between herpes viruses and periodontopathogens in hiv patients with periodontitis. | aim: to verify a possible association between herpes viruses and periodontal pathogens in individuals with human immunodeficiency virus (hiv+) and periodontitis. material and methods: twenty-seven hiv+ subjects with chronic periodontitis (cp) and 23 hiv+ patients with gingivitis (g) were included in the study. probing depth, clinical attachment level, gingival index, and plaque index were recorded. blood, saliva, and subgingival plaque were processed for viral and bacterial identification. bacte ... | 2011 | 21563945 |
| effectiveness of ozone against periodontal pathogenic microorganisms. | huth kc, quirling m, lenzke s, paschos e, kamereck k, brand k, hickel r, ilie n. effectiveness of ozone against periodontal pathogenic microorganisms. eur j oral sci 2011; 119: 204-210. ©2011 eur j oral sci ozone has been proposed as an adjunct antiseptic in periodontitis therapy. the aim of this study was to investigate the antimicrobial effectiveness of gaseous/aqueous ozone, in comparison with that of the established antiseptic chlorhexidine digluconate (chx), against periodontal microorganis ... | 2011 | 21564314 |
| tlr2-dependent modulation of osteoclastogenesis by porphyromonas gingivalis through differential induction of nfatc1 and nf-{kappa}b. | osteolytic diseases including rheumatoid arthritis, osteomyelitis and periodontitis are usually associated with bacterial infections. however, the precise mechanisms by which bacteria induce bone loss still remain unclear. evidence exists that toll-like receptor (tlr) signaling regulates both inflammation and bone metabolism and that the receptor activator of nf-κb ligand (rankl) and its receptor rank are the key regulators for bone remodeling and for the activation of osteoclasts. here, we inve ... | 2011 | 21566133 |
| antimicrobial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study. | becerik s, türkoğlu o, emingil g, vural c, özdemir g, atilla g. anti-microbial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study. apmis 2011; 119: 364-72. the aim of this study was to examine the effectiveness of chlorhexidine mouthrinse (chx) in addition to daily plaque control on subgingival microbiota in patients with untreated gingivitis. fifty gingivitis patients were randomized to chx or placebo groups. chx group rinsed wit ... | 2011 | 21569094 |
| diagnostic evaluation of a nanobody with picomolar affinity toward the protease rgpb from porphyromonas gingivalis. | porphyromonas gingivalis is one of the major periodontitis-causing pathogens. p. gingivalis secretes a group of proteases termed gingipains, and in this study we have used the rgpb gingipain as a biomarker for p. gingivalis. we constructed a naive camel nanobody library and used phage display to select one nanobody toward rgpb with picomolar affinity. the nanobody was used in an inhibition assay for detection of rgpb in buffer as well as in saliva. the nanobody was highly specific for rgpb given ... | 2011 | 21569755 |
| periodontopathogen and epstein-barr virus contamination affects transplanted bone volume in sinus augmentation. | background: bone microbial contamination can impair osteogenesis. human herpesviruses-associated vasculitis can cause vascular damage within the osseous graft and host. this study was conducted to substantiate specific contamination and assess the impact six months after sinus augmentation. methods: culture and polymerase chain reaction (pcr)-based identification were done on harvested bone particles and unstimulated whole saliva in a group of 30 patients undergoing maxillary sinus augmentation. ... | 2011 | 21574831 |
| [inhibitory effects of porphyromonas endodontalis lipopolysaccharides on proliferation and differentiation of mouse osteoblast]. | to observe the effect of lipopolysaccharides (lps) extracted from porphyromonas endodontalis (pe) on osteoblast cell proliferation and the activity of alkaline phosphatase (alp) and interleukin (il)-6 secretion and to investigate the role of pe-lps in osteoblast proliferation and differentiation. | 2011 | 21575438 |
| oral p. gingivalis infection alters the vascular reactivity in healthy and spontaneously atherosclerotic mice. | abstract: background: considering that recent studies have demonstrated endothelial dysfunction in subjects with periodontitis and that there is no information about vascular function in coexistence of periodontitis and atherosclerosis, we assessed the impact of oral inoculation with the periodontal pathogen porphyromonas gingivalis on vascular reactivity in healthy and hypercholesterolemic apolipoprotein e-deficient (apoe) mice. in vitro preparations of mesenteric arteriolar bed were used to de ... | 2011 | 21586133 |
| [cloning of the glyceraldehydes 3-phosphate dehydrogenase gene of porphyromonas gingivalis and its expression in e. coli]. | to clone the glyceraldehydes 3-phosphate dehydrogenase (gapdh) gene of porphyromonas gingivalis (p. gingivalis) and to induce its fusion expression in e. coli. | 2011 | 21598499 |
| ghrelin protects against the detrimental consequences of porphyromonas gingivalis-induced akt inactivation through s-nitrosylation on salivary mucin synthesis. | disturbances in nitric oxide synthase isozyme system and the impairment in salivary mucin synthesis are well-recognized features associated with oral mucosal inflammatory responses to periodontopathic bacterium, p. gingivalis. in this study, using rat sublingual gland acinar cells, we report that p. gingivalis lps-induced impairment in mucin synthesis and associated suppression in akt kinase activity were accompanied by a decrease in constitutive nitric oxide synthase (cnos) activity and an indu ... | 2011 | 21603133 |
| efficacy of taurolidine against periodontopathic species-an in vitro study. | the antimicrobial effect of taurolidine was tested against periodontopathic species in comparison to chlorhexidine digluconate in the presence or absence of serum. minimal inhibitory concentrations (mic), microbiocidal concentrations (mbc), as well as killing were determined against 32 different microbial strains including 3 porphyromonas gingivalis, 3 aggregatibacter actinomycetemcomitans, and 15 potentially superinfecting species with and without 25% v/v human serum. the mic(50) of taurolidine ... | 2011 | 21607568 |
| [effect of pg0839 gene of porphyromonas gingivalis on inflammatory cytokine in human oral epidermoid carcinoma kb cell.] | objective: to investigate the effect of pg0839 gene form porphyromonas gingivalis (pg) on inflammatory cytokine expression in human oral epidermoid carcinoma kb cell. methods: a mutant in the pg0839 gene of pg was created by insertional inactivation. group 1 was chanllenged with pgw83 strain, group 2 with pg0839-defective mutant, and the control group with dulbecco's modified eagle's medium only. kb cells were co-cultured with presence of bacteria for 24 h. at the time point of 0.5, 2, 6, 12 and ... | 2011 | 21612711 |
| identification of periodontal pathogens in healthy periimplant sites. | : the aim of this study was to evaluate the presence of periodontopathogens in subgingival periimplant sites in partially edentulous patients using polymerase chain reaction procedures, with regard to areas with clinical and radiographic signs of health and areas presenting periimplant disease. | 2011 | 21613949 |
| medical microbiology | bacteria are classified and identified to distinguish one organism from another and
to group similar organisms by criteria of interest to microbiologists or other
scientists. the most important level of this type of classification is the species
level. a species name should mean the same thing to everyone. within one species, strains
and subgroups can differ by the disease they produce, their environmental habitat,
a ... | 1996 | 21413329 |
| medical microbiology | at present there are over two dozen genera of gram-negative anaerobic bacilli. in most clinical infections, only the genera bacteroides, prevotella, and fusobacterium need be considered. these genera are prevalent in the body as members of the normal flora (fig.20-1), constituting one-third of the total anaerobic isolates from clinical specimens, and may become involved in infections throughout the body (fig.20-2). within the bacteroides group, b fragilis is the most common pathogen, followed b ... | 1996 | 21413336 |
| the evaluation of bacterial flora in progress of peri-implant disease. | background: cumulative interceptive supportive therapy (cist) is currently used as a guideline for treating peri-implant diseases. the objectives of this study were to determine the detection rate and measure the number of periodontopathic bacteria in lesions of different cist levels and thereby characterize peri-implant disease from a bacteriological viewpoint. methods: this study included 105 patients who had both residual natural teeth and implants with peri-implant disease. a total of 105 ... | 2011 | 21623813 |
| chronic oral infection with porphyromonas gingivalis accelerates atheroma formation by shifting the lipid profile. | recent studies have suggested that periodontal disease increases the risk of atherothrombotic disease. atherosclerosis has been characterized as a chronic inflammatory response to cholesterol deposition in the arteries. although several studies have suggested that certain periodontopathic bacteria accelerate atherogenesis in apolipoprotein e-deficient mice, the mechanistic link between cholesterol accumulation and periodontal infection-induced inflammation is largely unknown. | 2011 | 21625524 |
| comparison of bacterial adhesion and cellular proliferation on newly developed three-dimensional scaffolds manufactured by rapid prototyping technology. | scaffolds used in the field of tissue engineering should facilitate the adherence, spreading, and ingrowth of cells as well as prevent microbial adherence. for the first time, this study simultaneously deals with microbial and tissue cell adhesion to rapid prototyping-produced 3d-scaffolds. the cell growth of human osteosarcoma cells (cal-72) over a time period of 3-11 days were examined on three scaffolds (plga, plla, plla-tcp) and compared to the adhesion of salivary microorganisms and represe ... | 2011 | 21626662 |
| tlr2 signaling and th2 responses drive tannerella forsythia-induced periodontal bone loss. | periodontal disease (pd) is a chronic inflammation of the tooth-supporting soft tissue and alveolar bone due to infection by a select group of gram-negative microbes, which leads to tooth loss if untreated. because mice deficient in cd4(+) cells are resistant to infection-induced alveolar bone loss, th cells have been implicated in bone-destructive processes during pd. however, the extent to which different th cell subtypes play roles in pathogenesis or host protection remains to be defined and ... | 2011 | 21632710 |
| the pathogenic persona of community-associated oral streptococci. | the mitis group streptococci (mgs) are widespread in the oral cavity and are traditionally associated with oral health. however, these organisms have many attributes that contribute to the development of pathogenic oral communities. mgs adhere rapidly to saliva-coated tooth surfaces, thereby providing an attachment substratum for more overtly pathogenic organisms such as porphyromonas gingivalis, and the two species assemble into heterotypic communities. close physical association facilitates ph ... | 2011 | 21635580 |
| cell immobilization studies using a cellulose-binding domain fused to prtp in lactococcus lactis. | the cellulose-binding domain (cbd) of xyla was fused with prtp, nisp and acma anchors derived from lactococcus lactis under p45 promoter and usp45 secretion signal. the fusion construct with the anchor prtp (334 aa) was shown to exhibit the most efficient immobilization. the cbd-prtp construct on the other hand was not efficiently attached to the cell wall and as such was found mainly in the supernatant. results also showed that expression of the cbd-nisp anchor fusion led to a similar result. t ... | 2011 | 21637005 |
| suppression by ghrelin of porphyromonas gingivalis-induced constitutive nitric oxide synthase s-nitrosylation and apoptosis in salivary gland acinar cells. | oral mucosal inflammatory responses to periodontopathic bacterium, p. gingivalis, and its key virulence factor, lps, are characterized by a massive rise in epithelial cell apoptosis and the disturbances in no signaling pathways. here, we report that the lps-induced enhancement in rat sublingual salivary gland acinar cell apoptosis and no generation was associated with the suppression in constitutive nitric oxide synthase (cnos) activity and a marked increase in the activity of inducible nitric o ... | 2010 | 21637354 |
| characterization of human-type monoclonal antibodies against reduced form of hemin binding protein 35 from porphyromonas gingivalis. | shibata y, okano s, shiroza t, tahara t, nakazawa k, kataoka s, ishida i, kobayashi t, yoshie h, abiko y. characterization of human-type monoclonal antibodies against reduced form of hemin-binding protein 35 fromporphyromonas gingivalis. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01389.x.© 2011 john wiley & sons a/s background and objective: the gram-negative anaerobe porphyromonas gingivalis has been implicated as an important pathogen in the development of adult periodontitis, and it ... | 2011 | 21644999 |
| periodontopathogen- and host-derived immune response in acute coronary syndrome. | background: due to molecular mimicry, periodontal pathogen carriage may result in a systemic cross-reactive immune response with the host. objective: the analyses were performed to investigate if serum antibody levels to human heat-shock protein 60 (hsp60) are associated with the antibody levels and salivary carriage of two periodontal pathogens, aggregatibacter actinomycetemcomitans and porphyromonas gingivalis, as well as with the dental status in patients with acute coronary syndrome (acs). ... | 2011 | 21645027 |
| upregulation of heme oxygenase-1 via pi3k/akt and nrf-2 signaling pathways mediates the anti-inflammatory activity of schisandrin in porphyromonas gingivalis lps-stimulated macrophages. | the lipopolysaccharide (lps) of porphyromonas gingivalis is thought to induce periodontitis. in this study, we isolated schisandrin from the dried fruits of schisandra chinensis and examined the anti-inflammatory effect of schisandrion in macrophages stimulated with lps from p. gingivalis. first, schisandrin inhibited lps-induced pro-inflammatory cytokines, including tnf-α, il-1β, and il-6. and schisandrin suppressed the nuclear translocation and activity of nf-κb and phosphorylation of iκbα in ... | 2011 | 21645546 |
| association of natural anti-platelet factor 4/heparin antibodies with periodontal disease. | platelet factor 4 (pf4) and heparin (h) form pf4/h complexes, the target of the immune reaction in heparin-induced thrombocytopenia (hit). hit seems to be a secondary immune response as anti-pf4/h-igg antibodies occur as early as day 4 of heparin treatment. this study investigated whether prevalent infections such as periodontitis may induce the pf4/h immune response as: (1) natural anti-pf4/h abs are present in the normal population; (2) pf4 bound to bacteria exposes the same antigen(s) as pf4/ ... | 2011 | 21659541 |
| the lysine-specific gingipain of porphyromonas gingivalis : importance to pathogenicity and potential strategies for inhibition. | periodontitis is a disease affecting the supporting structures of the teeth. the most severe forms of the disease result in tooth loss and have recently been strongly associated with systemic diseases, including cardiovascular and lung diseases and cancer. the disease is caused by biofilms of predominantly anaerobic bacteria. a major pathogen associated with severe, adult forms of the disease is porphyromonas gingivalis. this organism produces potent cysteine proteases known as gingipains, which ... | 2011 | 21660656 |
| elevated antibody titers to porphyromonas gingivalis as a possible predictor of ischemic vascular disease. | aim: limited epidemiological studies have investigated the relationship between ischemic vascular disease and periodontitis in non-western populations. we investigated this relationship in a japanese cohort by measuring serum titers of antibodies to periodontopathic bacteria.methods: as part of the tokamachi-nakasato cohort study, we followed up 7021 participants regarding cardiovascular events over 5 years, and observed 99 ischemic vascular events: 66 cerebral infarctions and 33 cases of ischem ... | 2011 | 21670558 |
| the modular structure of haemagglutinin/adhesin regions in gingipains of porphyromonas gingivalis. | high-molecular-weight arginine- and lysine-specific (kgp) gingipains are essential virulence factors expressed by the oral pathogen porphyromonas gingivalis. haemagglutinin/adhesin (ha) regions of these proteases have been implicated in targeting catalytic domains to biological substrates and in other adhesive functions. we now report the crystal structure of the k3 adhesin domain/module of kgp, which folds into the distinct ß-jelly roll sandwich topology previously observed for k2. a conserved ... | 2011 | 21812842 |
| recovery of aerobic and anaerobic bacteria in sinus fungal ball. | this case study with chart review describes the aerobic and anaerobic microbiology of specimens obtained from 47 patients with aspergillus spp fungus ball. bacteria were recovered from 32 of the 47 (68%) patients. eighty-six isolates, 29 aerobic and facultatives and 57 anaerobic, were recovered. aerobic and facultatives only were recovered in 6 instances (19% of culture-positive specimens), anaerobes only in 11 (34%), and mixed aerobes and anaerobes in 15 (47%). the predominant aerobes were stap ... | 2011 | 21804027 |
| time-course gene expression during porphyromonas gingivalis strain atcc 33277 biofilm formation. | chronological gene-expression patterns of biofilm-forming cells are important to understand bioactivity and pathogenicity of biofilms. for porphyromonas gingivalis atcc 33277 biofilm formation, the number of genes differentially regulated by more than 1.5-fold was highest during the growth stage (312/2090 genes), and some pathogen-associated genes were time-dependently controlled. | 2011 | 21803908 |
| cysteine proteases from porphyromonas gingivalis and tlr ligands synergistically induce the synthesis of the cytokine il-8 in human artery endothelial cells. | objective: bacterial pathogens are frequently detected in atheromatous lesions, however, their contribution to atherosclerosis remains unknown. the present study was aimed to explore the effect of the p. gingivalis cysteine protease gingipain towards the proinflammatory response of human aortic endothelial cells (haecs). design: haecs were exposed to gingipains (rgps) extracted from the oral pathogen p. gingivalis. in addition, haecs were co-stimulated with the tlr ligands p. gingivalis lps, e. ... | 2011 | 21802653 |
| inhibitory effect of oral lactobacillus against oral pathogens. | aim: to determine the inhibitory effect of oral lactobacillus against putative oral pathogens. methods and results: total 357 strains comprising 10 species of oral lactobacillus, lactobacillus fermentum (195), lactobacillus salivarius (53), lactobacillus casei (20), lactobacillus gasseri (18), lactobacillus rhamnosus (14), lactobacillus paracasei (12), lactobacillus mucosae (12), lactobacillus oris (12), lactobacillus plantarum (11) and lactobacillus vaginalis (10) were used as producer strain ... | 2011 | 21801186 |
| prevotella nigrescens and porphyromonas gingivalis are associated with signs of carotid atherosclerosis in subjects with and without periodontitis. | yakob m, söder b, meurman jh, jogestrand t, nowak j, söder p.-ö. prevotella nigrescens and porphyromonas gingivalis are associated with signs of carotid atherosclerosis in subjects with and without periodontitis. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01398.x. © 2011 john wiley & sons a/s background and objective: oral microorganisms may be involved in the development of cardiovascular diseases, and porphyromonas gingivalis is one of the periodontal microorganisms that has been fou ... | 2011 | 21793826 |
| blockade of tlr2 inhibits porphyromonas gingivalis suppression of mineralized matrix formation by human dental pulp stem cells. | human dental pulp stem/progenitor cells (hdpsc) can differentiate into odontoblast-like cells and express dentin sialophosphoprotein (dspp) and osteocalcin (ocn); thus, they may be used to regenerate dentin. however, residual bacterial components in the root canal may suppress this activity. | 2011 | 21787495 |
| mutations in flavobacterium johnsoniae spre result in defects in gliding motility and protein secretion. | cells of the gliding bacterium flavobacterium johnsoniae move rapidly over surfaces. transposon mutagenesis was used to identify spre, which is involved in gliding. mutations in spre resulted in the formation of nonspreading colonies on agar. spre mutant cells in wet mounts were almost completely deficient in attachment to and movement on glass, but a small percentage of cells exhibited slight movements, indicating that the motility machinery was not completely disrupted. spre is a predicted lip ... | 2011 | 21784937 |
| detection of periodontal markers in chronic periodontitis. | the aim was to compare the detection frequency of periodontopathogens by using the pado test 4.5 and checkerboard dna-dna hybridization technique in chronic periodontitis patients.thirty patients with chronic periodontitis were tested cross-sectionally with dna/rna oligogenomic probe method (iai pado test 4.5) and dna/dna whole genomic probe (checkerboard) method. samples were taken by two paper points at the deepest site in each of the four quadrants and pooled into one sample for each of the t ... | 2011 | 21769304 |
| cleavage of igg1 and igg3 by gingipain k from porphyromonas gingivalis may compromise host defense in progressive periodontitis. | degradation of immunoglobulins is an effective strategy of bacteria to evade the immune system. we have tested whether human igg is a substrate for gingipain k of porphyromonas gingivalis and found that the enzyme can hydrolyze subclass 1 and 3 of human igg. the heavy chain of igg(1) was cleaved at a single site within the hinge region, generating fab and fc fragments. igg(3) was also cleaved within the heavy chain, but at several sites around the ch2 region. investigation of the enzyme kinetics ... | 2011 | 21768393 |
| porphyromonas gingivalis lipopolysaccharide lipid a heterogeneity differentially modulates the expression of il-6 and il-8 in human gingival fibroblasts. | herath tdk, wang y, seneviratne cj, lu q, darveau rp, wang cy, jin l. porphyromonas gingivalis lipopolysaccharide lipid a heterogeneity differentially modulates the expression of il-6 and il-8 in human gingival fibroblasts. j clin periodontol 2011; 38: 694-701. doi: 10.1111/j.1600-051x.2011.01741.x. abstract: aim: porphyromonas gingivalis lipopolysaccharide (lps) displays a significant amount of structural heterogeneity, containing both tetra- (lps(1435/1449) ) and penta-acylated (lps(1690) ) li ... | 2011 | 21752043 |
| histone deacetylase inhibitors and periodontal bone loss. | cantley md, bartold pm, marino v, fairlie dp, le gt, lucke aj, haynes dr. histone deacetylase inhibitors and periodontal bone loss. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01392.x.©2011 john wiley & sons a/s background and objective: bone loss caused by enhanced osteoclast activity is a significant feature of periodontitis. histone deacetylase inhibitors (hdaci) can suppress osteoclast-mediated bone loss in vitro and in vivo. this study investigated whether hdaci can suppress bone l ... | 2011 | 21745207 |
| antibacterial susceptibility patterns of porphyromonas gingivalis isolated from chronic periodontitis patients. | objectives: to test the antimicrobial sensitivity of porphyromonas gingivalis to a panel of eight orally administrable antibiotics in chronic periodontal diseases and to evaluate factors associated with periodontitis in adult patients. study design: a total of fifty strains of p. gingivalis were isolated from one hundred and twenty adult patients with chronic perio-dontitis. identification of bacteria was carried out by anaerobic culture and biochemical tests. selected colonies of p. gingivalis ... | 2011 | 21743419 |
| analysis of the peri-implant microbiota in 90 dental implants and its relationship to crevicular fluid volume. | objective: to evaluate the presence within the peri-implant sulcus of tannerela forsythia (tf), porphyromonas gingivales (pg), treponema denticola (td) and aggregatibacter actinomycetemcomitans (aa), and relate these bacteria to the peri-implant crevicular fluid volume (picfv). material and method: a prospective and cross-sectional clinical case series study was made. for the measurement of crevicular fluid, use was made of the periotron® 8000 (proflow incorporated. new york, usa), measuring the ... | 2011 | 21743411 |
| altered toll-like receptor 2-mediated endotoxin tolerance is related to diminished interferon {beta} production. | induction of endotoxin tolerance leads to a reduced inflammatory response after repeated challenge by lps and is important for resolution of inflammation and prevention of tissue damage. enterobacterial lps is recognized by the tlr4 signaling complex, whereas lps of some non-enterobacterial organisms is capable of signaling independently of tlr4 utilizing tlr2-mediated signal transduction instead. in this study we report that porphyromonas gingivalis lps, a tlr2 agonist, fails to induce a fully ... | 2011 | 21705332 |
| candida albicans enhances invasion of human gingival epithelial cells and gingival fibroblasts by porphyromonas gingivalis. | although candida albicans has been isolated from periodontal pockets, its relationship to periodontitis is unclear. in this study, we investigated the effect of c.-áalbicans on the adhesion and invasion of ca9-22, a human gingival epithelial cell line, and human gingival fibroblasts by porphyromonas gingivalis. heat-killed c.-áalbicans and water-soluble mannoprotein-+¦-glucan complex from c.-áalbicans (caws) did not enhance p.-ágingivalis adhesion or upregulate the expression of +¦1 integrin and ... | 2011 | 21742026 |
| effect of melatonin on cyclooxygenase-2 expression and nuclear factor-kappa b activation in raw264.7 macrophage-like cells stimulated with fimbriae of porphyromonas gingivalis. | the possible link between melatonin and anti-inflammatory activity is currently a focus of interest. in the present study, cox-2 expression and nf-+¦b activation in raw264.7 macrophage-like cells stimulated with the fimbriae of porphyromonas gingivalis, an oral anaerobe, in the absence and presence of melatonin were investigated. | 2011 | 21709008 |
| porphyromonas gingivalis-mediated shedding of extracellular matrix metalloproteinase inducer (emmprin) by oral epithelial cells: a potential role in inflammatory periodontal disease. | extracellular matrix metalloproteinase inducer (emmprin) or cd147 is a transmembrane glycoprotein expressed by various cell types, including oral epithelial cells. recent studies have brought evidence that emmprin plays a role in periodontitis. in the present study, we investigated the effect of porphyromonas gingivalis, a major pathogen in chronic periodontitis, on the shedding of membrane-anchored emmprin and on the expression of the emmprin gene by oral epithelial cells. a potential contribut ... | 2011 | 21835259 |
| efficacy of chlorhexidine digluconate-containing formulations and other mouthrinses against periodontopathogenic microorganisms. | objective: to determine in vitro the action of chlorhexidine digluconate and different commercially available mouthrinses on oral microorganisms. method and materials: minimal inhibitory concentrations and possible induction of resistance by chlorhexidine digluconate, an essential oil-containing mouthwash and an amine fluoride/stannous fluoride solution, were determined against microorganisms normally found in the oral cavity (10 streptococci, 2 enterobacteria, 1 candida albicans, 8 porphyromona ... | 2011 | 21842009 |
| iron(iii) mesoporphyrin ix and iron(iii) deuteroporphyrin ix bind to the porphyromonas gingivalis hmuy hemophore. | porphyromonas gingivalis acquires heme through an outer-membrane heme transporter hmur and heme-binding hemophore-like lipoprotein hmuy. here, we compare binding of iron(iii) mesoporphyrin ix (mesoheme) and iron(iii) deuteroporphyrin ix (deuteroheme) to hmuy with that of iron(iii) protoporphyrin ix (protoheme) and protoporphyrin ix (ppix) using spectroscopic methods. in contrast to ppix, mesoheme and deuteroheme enter the hmuy heme cavity and are coordinated by his134 and his166 residues in a fu ... | 2011 | 21740890 |
| microbiologic changes in subgingival plaque before and during the early period of orthodontic treatment. | abstract objective: to evaluate changes in subgingival microbiota before and during the leveling and alignment orthodontic stage using the polymerase chain reaction (pcr) method. materials and methods: thirty orthodontic patients (17 females and 13 males; aged 16.7 -¦ 6.5-áy) were included in this study. subgingival microbial samples were taken from the disto-buccal gingival crevice of the left upper central incisors, the left lower central incisors, the mesio-buccal gingival crevice of the left ... | 2011 | 21827233 |
| aerosol deposition of hydroxyapatite and 4-hexylresorcinol coatings on titanium alloys for dental implants. | purpose: aerosol deposition is a newly developed technique, and it can deliver the drug from a hydroxyapatite (ha)-coated surface. 4-hexylresorcinol (4-hr) is a well-known antiseptic. the influence of the 4-hr component of ha coatings on titanium surfaces was studied in vitro and in vivo. materials and methods: x-ray diffraction and fourier transform infrared techniques were used for the evaluation of the coating. the cellular response of the coating was evaluated by scanning electron microscopi ... | 2011 | 21821331 |
| por secretion system-dependent secretion and glycosylation of porphyromonas gingivalis hemin-binding protein 35. | the anaerobic gram-negative bacterium porphyromonas gingivalis is a major pathogen in severe forms of periodontal disease and refractory periapical perodontitis. we have recently found that p. gingivalis has a novel secretion system named the por secretion system (porss), which is responsible for secretion of major extracellular proteinases, arg-gingipains (rgps) and lys-gingipain. these proteinases contain conserved c-terminal domains (ctds) in their c-termini. hemin-binding protein 35 (hbp35), ... | 2011 | 21731719 |
| [prevalence of helicobacter pylori detected by real-time pcr in the subgingival plaque of patients with chronic periodontitis]. | helicobacter pylori plays a significant role in gastric disease. the aim of the present pilot study was to evaluate the prevalence of h. pylori detected by real-time polymerase chain reaction (real-time pcr) in the sub-gingival plaque of chronic periodontitis patients and to determine periodontopathogens profile of positive sites at h. pylori. | 2011 | 21688602 |
| influence of gaseous ozone in peri-implantitis: bactericidal efficacy and cellular response. an in vitro study using titanium and zirconia. | dental implants are prone to bacterial colonization which may result in bone destruction and implant loss. treatments of peri-implant disease aim to reduce bacterial adherence while leaving the implant surface intact for attachment of bone-regenerating host cells. the aims of this study were to investigate the antimicrobial efficacy of gaseous ozone on bacteria adhered to various titanium and zirconia surfaces and to evaluate adhesion of osteoblast-like mg-63 cells to ozone-treated surfaces. sal ... | 2011 | 21842144 |
| clinical, microbiologic, and immunologic factors of orthodontic treatment-induced gingival enlargement. | the aim of this study was to investigate the microbiologic and immunologic factors related to orthodontic treatment-induced gingival enlargement (ge). | 2011 | 21724088 |
| the elicitation step of nickel allergy is promoted in mice by microbe-related substances, including some from oral bacteria. | microbial components activate the host's innate immunity via interactions with molecules including tlrs and nods. we previously reported that in mice (i) escherichia coli lipopolysaccharide (lps; tlr4 agonist) promotes ni-allergy even in t-cell-deficient mice, (ii) e. coli lps reduces the minimum allergy-inducing concentrations of ni at both the sensitization and elicitation steps, and (iii) various microbe-related substances promote sensitization to ni. here, we examined the effects of microbe- ... | 2011 | 21839857 |
| detection of porphyromonas gulae from subgingival biofilms of dogs with and without periodontitis. | a rapid pcr approach was developed to detect porphyromonas gulae strains from subgingival samples of dogs with and with periodontitis. the presence of p.-águlae was observed in 92% and 56%, respectively, in dogs with and without periodontitis. the new primer pair was specific to detect this microorganism, and this technique could be used to evaluate a correlation between periodontitis and p.-águlae in companion animals. | 2011 | 21723404 |
| phenotypic identification of over 1000 isolates of anaerobic bacteria recovered between 1999 and 2008 in a major costa rican hospital. | because of limitations in infrastructure, the aetiology of infections caused by anaerobic bacteria is seldom determined in clinical laboratories of developing countries. this study reports on the identification of 1010 anaerobic bacterial isolates collected between 1999 and 2008 in a major costa rican hospital with the use of two commercial phenotypic systems (rapid 32a and api 20a). approximately 60% of the isolates were gram-positive and, among the 35 species of gram-positive bacteria found, t ... | 2011 | 21722256 |
| synergistic virulence of porphyromonas gingivalis and treponema denticola in a murine periodontitis model. | chronic periodontitis is characterized by the destruction of the tissues supporting the teeth and has been associated with the presence of a subgingival polymicrobial biofilm containing porphyromonas gingivalis and treponema denticola. we have investigated the potential synergistic virulence of p. gingivalis and t. denticola using a murine experimental model of periodontitis. an inoculation regime of four intra-oral doses of 1 +ù 10(10) p. gingivalis cells induced significant periodontal bone lo ... | 2011 | 21729244 |
| b7-h1 and b7-dc receptors of oral squamous carcinoma cells are upregulated by porphyromonas gingivalis. | the up-regulation of the b7-h1 receptors in host cells might influence the chronicity of inflammatory disorders that frequently precede the development of human cancers. b7-h1 expression has been detected in the majority of human cancers, leading to anergy and apoptosis of activated t cells, and enabling tumor cells to overcome host response. porphyromonas gingivalis (p. gingivalis), a putative periodontal pathogen, is an etiologic agent of periodontitis and expresses a variety of virulence fact ... | 2011 | 21723642 |