| customizable pcr-microplate array for differential identification of multiple pathogens. | customizable pcr-microplate arrays were developed for the rapid identification of salmonella typhimurium, salmonella saintpaul, salmonella typhi, shigella dysenteriae, escherichia coli o157:h7, francisella tularensis subsp. tularensis, francisella tularensis subsp. novicida, vibrio cholerae, vibrio parahaemolyticus, yersinia pestis, and yersinia pseudotuberculosis. previously, we identified highly specific primers targeting each of these pathogens. here, we report the development of customizable ... | 2013 | 24215700 |
| fis is essential for yersinia pseudotuberculosis virulence and protects against reactive oxygen species produced by phagocytic cells during infection. | all three pathogenic yersinia species share a conserved virulence plasmid that encodes a type 3 secretion system (t3ss) and its associated effector proteins. during mammalian infection, these effectors are injected into innate immune cells, where they block many bactericidal functions, including the production of reactive oxygen species (ros). however, y. pseudotuberculosis (yptb) lacking the t3ss retains the ability to colonize host organs, demonstrating that chromosome-encoded factors are suff ... | 2016 | 27689357 |
| cold shock proteins: a minireview with special emphasis on csp-family of enteropathogenic yersinia. | bacteria have evolved a number of mechanisms for coping with stress and adapting to changing environmental conditions. many bacteria produce small cold shock proteins (csp) as a response to rapid temperature downshift (cold shock). during cold shock, the cell membrane fluidity and enzyme activity decrease, and the efficiency of transcription and translation is reduced due to stabilization of nucleic acid secondary structures. moreover, protein folding is inefficient and ribosome function is hamp ... | 2016 | 27499753 |
| retracing the evolutionary path that led to flea-borne transmission of yersinia pestis. | yersinia pestis is an arthropod-borne bacterial pathogen that evolved recently from yersinia pseudotuberculosis, an enteric pathogen transmitted via the fecal-oral route. this radical ecological transition can be attributed to a few discrete genetic changes from a still-extant recent ancestor, thus providing a tractable case study in pathogen evolution and emergence. here, we determined the genetic and mechanistic basis of the evolutionary adaptation of y. pestis to flea-borne transmission. rema ... | 2014 | 24832452 |
| a conjugative 38 kb plasmid is present in multiple subspecies of xylella fastidiosa. | a ≈ 38kb plasmid (pxf-riv5) was present in the riv5 strain of xylella fastidiosa subsp. multiplex isolated from ornamental plum in southern california. the complete nucleotide sequence of pxf-riv5 is almost identical to that of pxfas01 from x. fastidiosa subsp. fastidiosa strain m23; the two plasmids vary at only 6 nucleotide positions. blast searches and phylogenetic analyses indicate pxf-riv5 and pxfas01 share some similarity to chromosomal and plasmid (pxf51) sequences of x. fastidiosa subsp. ... | 2012 | 23251694 |
| genome-wide analysis of small rnas expressed by yersinia pestis identifies a regulator of the yop-ysc type iii secretion system. | small noncoding rna (srna) molecules are integral components of the regulatory machinery for many bacterial species and are known to posttranscriptionally regulate metabolic and stress-response pathways, quorum sensing, virulence factors, and more. the yop-ysc type iii secretion system (t3ss) is a critical virulence component for the pathogenic yersinia species, and the regulation of this system is tightly controlled at each step from transcription to translocation of effectors into host cells. ... | 2014 | 24532772 |
| crp induces switching of the csrb and csrc rnas in yersinia pseudotuberculosis and links nutritional status to virulence. | colonization of the intestinal tract and dissemination into deeper tissues by the enteric pathogen yersinia pseudotuberculosis demands expression of a special set of virulence factors important for the initiation and the persistence of the infection. in this study we demonstrate that many virulence-associated functions are coregulated with the carbohydrate metabolism. this link is mediated by the carbon storage regulator (csr) system, including the regulatory rnas csrb and csrc, and the camp rec ... | 2012 | 23251905 |
| yopj-induced caspase-1 activation in yersinia-infected macrophages: independent of apoptosis, linked to necrosis, dispensable for innate host defense. | yersinia outer protein j (yopj) is a type iii secretion system (t3ss) effector of pathogenic yersinia (yersinia pestis, yersinia enterocolitica and yersinia pseudotuberculosis) that is secreted into host cells. yopj inhibits survival response pathways in macrophages, causing cell death. allelic variation of yopj is responsible for differential cytotoxicity in yersinia strains. yopj isoforms in y. enterocolitica o:8 (yopp) and y. pestis kim (yopj(kim)) strains have high cytotoxic activity. in add ... | 2012 | 22563435 |
| caspase-11 activation in response to bacterial secretion systems that access the host cytosol. | inflammasome activation is important for antimicrobial defense because it induces cell death and regulates the secretion of il-1 family cytokines, which play a critical role in inflammatory responses. the inflammasome activates caspase-1 to process and secrete il-1β. however, the mechanisms governing il-1α release are less clear. recently, a non-canonical inflammasome was described that activates caspase-11 and mediates pyroptosis and release of il-1α and il-1β. caspase-11 activation in response ... | 2013 | 23762026 |
| the gap activity of type iii effector yope triggers killing of yersinia in macrophages. | the mammalian immune system has the ability to discriminate between pathogens and innocuous microbes by detecting conserved molecular patterns. in addition to conserved microbial patterns, the mammalian immune system may recognize distinct pathogen-induced processes through a mechanism which is poorly understood. previous studies have shown that a type iii secretion system (t3ss) in yersinia pseudotuberculosis leads to decreased survival of this bacterium in primary murine macrophages by unknown ... | 2014 | 25165815 |
| a direct link between the global regulator phop and the csr regulon in y. pseudotuberculosis through the small regulatory rna csrc. | in this study we investigated the influence of the global response regulator phop on the complex regulatory cascade controlling expression of early stage virulence genes of yersinia pseudotuberculosis via the virulence regulator rova. our analysis revealed the following novel features: (1) phop activates expression of the csrc rna in y. pseudotuberculosis, leading to activation of rova synthesis through the csrabc-rovm cascade, (2) activation of csrc transcription is direct and phop is shown to ... | 2014 | 24786463 |
| natural killer cells mediate protection against yersinia pseudotuberculosis in the mesenteric lymph nodes. | natural killer cells play a crucial role in the initial defense against bacterial pathogens. the crosstalk between host cells infected with intracellular pathogens and nk cells has been studied intensively, but not much attention has been given to characterize the role of nk cells in the response to extracellular bacterial pathogens such as yersiniae. in this study we used antibody-mediated nk cell depletion to address the importance of this immune cell type in controlling a y. pseudotuberculosi ... | 2015 | 26296209 |
| yersinia infection tools-characterization of structure and function of adhesins. | among the seventeen species of the gram-negative genus yersinia, three have been shown to be virulent and pathogenic to humans and animals-y. enterocolitica, y. pseudotuberculosis, and y. pestis. in order to be so, they are armoured with various factors that help them adhere to tissues and organelles, cross the cellular barrier and escape the immune system during host invasion. the group of proteins that mediate pathogen-host interactions constitute adhesins. invasin, ail, yada, yadb, yadc, pla, ... | 2012 | 23316485 |
| diversification of β-augmentation interactions between cdi toxin/immunity proteins. | contact-dependent growth inhibition (cdi) is a widespread mechanism of inter-bacterial competition mediated by the cdib/cdia family of two-partner secretion proteins. cdia effectors carry diverse c-terminal toxin domains (cdia-ct), which are delivered into neighboring target cells to inhibit growth. cdi(+) bacteria also produce cdii immunity proteins that bind specifically to cognate cdia-ct toxins and protect the cell from auto-inhibition. here, we compare the structures of homologous cdia-ct/c ... | 2015 | 26449640 |
| expression and targeting of secreted proteins from chlamydia trachomatis. | chlamydia trachomatis is an obligate intracellular pathogen that replicates in a vacuole termed the inclusion. many of the interactions of chlamydiae with the host cell are dependent upon bacterial protein synthesis and presumably exposure of these proteins to the cytosol. because of the dearth of genetic tools for chlamydiae, previous studies examining secreted proteins required the use of heterologous bacterial systems. recent advances in genetic manipulation of chlamydia now allow for transfo ... | 2014 | 24443531 |
| interactions of the cpxa sensor kinase and cognate cpxr response regulator from yersinia pseudotuberculosis. | the cpxa sensor kinase-cpxr response regulator two-component regulatory system is a sentinel of bacterial envelope integrity. integrating diverse signals, it can alter the expression of a wide array of components that serve to shield the envelope from damage and to promote bacterial survival. in bacterial pathogens such as yersinia pseudotuberculosis, this also extends to pathogenesis. cpxr is thought to dimerize upon phosphorylation by the sensor kinase cpxa. this phosphorylation enables cpxr b ... | 2012 | 23013530 |
| evaluation of a single procedure allowing the isolation of enteropathogenic yersinia along with other bacterial enteropathogens from human stools. | enteropathogenic yersinia are among the most frequent agents of human diarrhea in temperate and cold countries. however, the incidence of yersiniosis is largely underestimated because of the peculiar growth characteristics of pathogenic yersinia, which make their isolation from poly-contaminated samples difficult. the use of specific procedures for yersinia isolation is required, but is expensive and time consuming, and therefore is not systematically performed in clinical pathology laboratories ... | 2012 | 22911756 |
| aerobic bacterial community of american cockroach periplaneta americana,a step toward finding suitable paratransgenesis candidates. | cockroaches mechanically spread pathogenic agents, however, little is known about their gut microbiota. identification of midgut microbial community helps targeting novel biological control strategies such as paratransgenesis. here the bacterial microbiota of periplaneta americana midgut, were identified and evaluated for finding proper paratransgenesis candidate. | 2014 | 26114142 |
| enzymatic synthesis of bioinformatically predicted microcin c-like compounds encoded by diverse bacteria. | abstract the trojan horse escherichia coli antibiotic microcin c (mcc) consists of a heptapeptide attached to adenosine through a phosphoramidate linkage. mcc is synthesized by the mccb enzyme, which terminally adenylates the ribosomally synthesized heptapeptide precursor mcca. the peptide part is responsible for mcc uptake; it is degraded inside the cell to release a toxic nonhydrolyzable aspartyl-adenylate. bionformatic analysis reveals that diverse bacterial genomes encoding mccb homologues a ... | 2014 | 24803518 |
| the resveratrol tetramer (-)-hopeaphenol inhibits type iii secretion in the gram-negative pathogens yersinia pseudotuberculosis and pseudomonas aeruginosa. | society faces huge challenges, as a large number of bacteria have developed resistance towards many or all of the antibiotics currently available. novel strategies that can help solve this problem are urgently needed. one such strategy is to target bacterial virulence, the ability to cause disease e.g., by inhibition of type iii secretion systems (t3sss) utilized by many clinically relevant gram-negative pathogens. many of the antibiotics used today originate from natural sources. in contrast, m ... | 2013 | 24324737 |
| comparative immunogenicity and structural analysis of epitopes of different bacterial l-asparaginases. | e.coli type ii l-asparaginase is widely used for treatment of acute lymphoblastic leukemia. however, serious side effects such as allergic or hypersensitivity reactions are common for l-asparaginase treatment. methods for minimizing immune response on l-asparaginase treatment in human include bioengeneering of less immunogenic version of the enzyme or utilizing the homologous enzymes of different origin. to rationalize these approaches we compared immunogenicity of l-asparaginases from five bact ... | 2016 | 26867931 |
| genetically engineered frameshifted yopn-tyea chimeras influence type iii secretion system function in yersinia pseudotuberculosis. | type iii secretion is a tightly controlled virulence mechanism utilized by many gram negative bacteria to colonize their eukaryotic hosts. to infect their host, human pathogenic yersinia spp. translocate protein toxins into the host cell cytosol through a preassembled ysc-yop type iii secretion device. several of the ysc-yop components are known for their roles in controlling substrate secretion and translocation. particularly important in this role is the yopn and tyea heterodimer. in this stud ... | 2013 | 24098594 |
| iscr is essential for yersinia pseudotuberculosis type iii secretion and virulence. | type iii secretion systems (t3ss) are essential for virulence in dozens of pathogens, but are not required for growth outside the host. therefore, the t3ss of many bacterial species are under tight regulatory control. to increase our understanding of the molecular mechanisms behind t3ss regulation, we performed a transposon screen to identify genes important for t3ss function in the food-borne pathogen yersinia pseudotuberculosis. we identified two unique transposon insertions in yptb2860, a gen ... | 2014 | 24945271 |
| a lysr-type transcriptional regulator, rovm, senses nutritional cues suggesting that it is involved in metabolic adaptation of yersinia pestis to the flea gut. | yersinia pestis has evolved as a clonal variant of yersinia pseudotuberculosis to cause flea-borne biofilm-mediated transmission of the bubonic plague. the lysr-type transcriptional regulator, rovm, is highly induced only during y. pestis infection of the flea host. rovm homologs in other pathogens regulate biofilm formation, nutrient sensing, and virulence; including in y. pseudotuberculosis, where rovm represses the major virulence factor, rova. here the role that rovm plays during flea infect ... | 2015 | 26348850 |
| microbiota-dependent sequelae of acute infection compromise tissue-specific immunity. | infections have been proposed as initiating factors for inflammatory disorders; however, identifying associations between defined infectious agents and the initiation of chronic disease has remained elusive. here, we report that a single acute infection can have dramatic and long-term consequences for tissue-specific immunity. following clearance of yersinia pseudotuberculosis, sustained inflammation and associated lymphatic leakage in the mesenteric adipose tissue deviates migratory dendritic c ... | 0 | 26451485 |
| prevalence of yersinia enterocolitica and yersinia pseudotuberculosis in wild boars in the basque country, northern spain. | yersiniosis is a zoonosis widely distributed in europe and swine carry different serotypes of yersinia enterocolitica and y. pseudotuberculosis. the aim of this study was to determine the prevalence of y. enterocolitica and y. pseudotuberculosis in wild boars in northern spain. the blood of wild boars (n = 505) was sampled between 2001 and 2012. seroprevalence was determined in 490 serum samples with an indirect enzyme-linked immunosorbent assay. seventy-two of the animals were also examined for ... | 2016 | 26790625 |
| cluster analysis of host cytokine responses to biodefense pathogens in a whole blood ex vivo exposure model (weem). | rapid detection and therapeutic intervention for infectious and emerging diseases is a major scientific goal in biodefense and public health. toward this end, cytokine profiles in human blood were investigated using a human whole blood ex vivo exposure model, called weem. | 2012 | 22607329 |
| design and characterization of novel hybrid antimicrobial peptides based on cecropin a, ll-37 and magainin ii. | antimicrobial peptides (amps) are a naturally occurring component of the innate immune response of many organisms and can have activity against both gram-negative and gram-positive bacterial species. in order to optimize and improve the direct antimicrobial effect of amps against a broad spectrum of bacterial species, novel synthetic hybrids were rationally designed from cecropin a, ll-37 and magainin ii. amps were selected based on their α-helical secondary structure and fragments of these were ... | 2012 | 22289499 |
| kawasaki disease-specific molecules in the sera are linked to microbe-associated molecular patterns in the biofilms. | kawasaki disease (kd) is a systemic vasculitis of unknown etiology. the innate immune system is involved in its pathophysiology at the acute phase. we have recently established a novel murine model of kd coronary arteritis by oral administration of a synthetic microbe-associated molecular pattern (mamp). on the hypothesis that specific mamps exist in kd sera, we have searched them to identify kd-specific molecules and to assess the pathogenesis. | 2014 | 25411968 |
| in vitro evaluation of antimicrobial features of vasopressors. | drugs administered as intravenous infusion may be contaminated during several stages of production or preparation. however studies focusing on antibacterial effects of vasopressor drugs are very rare. this study investigates the in vitro antimicrobial activity of the clinically used forms of vasopressors. | 2014 | 24794449 |
| conventional and molecular methods in the diagnosis of community-acquired diarrhoea in children under 5 years of age from the north-eastern region of poland. | the purpose of this study was to determine the main causative agents of community-acquired acute diarrhoea in children using conventional methods and pcr. | 2015 | 26159845 |
| [identification of zoonotic bacterial pathogens by the maldi tof ms method]. | to verify whether the maldi tof ms method can be used for rapid identification of selected zoonotic bacterial pathogens isolated from various types of materials in the real conditions of routine laboratory work. | 2015 | 26312374 |
| invasive escherichia coli vaccines expressing brucella melitensis outer membrane proteins 31 or 16 or periplasmic protein bp26 confer protection in mice challenged with b. melitensis. | because of the serious economic and medical consequences of brucellosis, efforts are to prevent infection of domestic animals through vaccines. many disadvantages are associated with the current brucella melitensis rev.1 vaccine prompting development of alternative vaccines and delivery. escherichia coli (dh5α) was engineered to express a plasmid containing the inv gene from yersinia pseudotuberculosis and the hly gene from listeria monocytogenes. these recombinant invasive e. coli expressing b. ... | 2012 | 22546330 |
| zoonotic public health hazards in backyard chickens. | backyard poultry has become increasingly popular in industrialized countries. in addition to keeping chickens for eggs and meat, owners often treat the birds as pets. however, several pathogenic enteric bacteria have the potential for zoonotic transmission from poultry to humans but very little is known about the occurrence of zoonotic pathogens in backyard flocks. the occurrence and the antimicrobial resistance of salmonella enterica, campylobacter spp., listeria monocytogenes and enteropathoge ... | 2016 | 26752227 |
| abortion in small ruminants in the netherlands between 2006 and 2011. | during five successive lambing seasons between 2006 and 2011, 453 submissions of abortion material, 282 of ovine and 171 of caprine origin, were examined at the animal health service in the netherlands. infectious agents as the most plausible cause of the abortion were found in 48 percent of the ovine submissions and in 34 percent of the caprine submissions. submission of both aborted fetus and placental membranes increased the diagnostic yield of laboratory investigations (17 percent and 21 per ... | 2012 | 22900421 |
| design, synthesis and evaluation of novel polypharmacological antichlamydial agents. | discovery of new polypharmacological antibacterial agents with multiple modes of actions can be an alternative to combination therapy and also a possibility to slow development of antibiotic resistance. in support to this hypothesis, we synthesized 16 compounds by combining the pharmacophores of chlamydia trachomatis inhibitors and inhibitors of type iii secretion (t3s) in gram-negative bacteria. in this study we have developed salicylidene acylhydrazide sulfonamides (11c &11d) as new antichlamy ... | 2015 | 26204507 |
| derivatives of 8-hydroxyquinoline--antibacterial agents that target intra- and extracellular gram-negative pathogens. | small molecule screening identified 5-nitro-7-((4-phenylpiperazine-1-yl-)methyl)quinolin-8-ol inp1750 as a putative inhibitor of type iii secretion (t3s) in the gram-negative pathogen yersinia pseudotuberculosis. in this study we report structure-activity relationships for inhibition of t3s and show that the most potent compounds target both the extracellular bacterium y. pseudotuberculosis and the intracellular pathogen chlamydia trachomatis in cell-based infection models. | 2012 | 22525317 |
| transcriptional frameshifting rescues citrobacter rodentium type vi secretion by the production of two length variants from the prematurely interrupted tssm gene. | the type vi secretion system (t6ss) mediates toxin delivery into both eukaryotic and prokaryotic cells. it is composed of a cytoplasmic structure resembling the tail of contractile bacteriophages anchored to the cell envelope through a membrane complex composed of the tssl and tssm inner membrane proteins and of the tssj outer membrane lipoprotein. the c-terminal domain of tssm is required for its interaction with tssj, and for the function of the t6ss. in citrobacter rodentium, the tssm1 gene d ... | 2014 | 25474156 |
| expression and cytoprotective activity of the small gtpase rhob induced by the escherichia coli cytotoxic necrotizing factor 1. | rhob is the only member of the rho subfamily of small gtpases, which is classified as an immediate early gene product. rhob is up-regulated in response to growth factors as well as cytotoxic and genotoxic agents. clostridial glucosylating toxins have been reported to evoke pronounced rhob expression, based on the inactivation of rho/ras proteins. in this study, we report on a long lasting expression of rhob in cultured cells upon activation of rho proteins by the cytotoxic necrotizing factor 1 ( ... | 2013 | 23732113 |
| roles of rpos in yersinia pseudotuberculosis stress survival, motility, biofilm formation and type vi secretion system expression. | rpos (σ(s)), the stationary phase/stress σ factor, controls the expression of a large number of genes involved in cellular responses to a variety of stresses. however, the role of rpos appears to differ in different bacteria. while rpos is an important regulator of flagellum biosynthesis, it is associated with biofilm development in edwardsiella tarda. biofilms are dense communities formed by bacteria and are important for microbe survival under unfavorable conditions. the type vi secretion syst ... | 2015 | 26310305 |
| microwave-assisted preparation of azachalcones and their n-alkyl derivatives with antimicrobial activities. | two new azachalcones were prepared by both claisen-schmidt condensation and a simple environmentally trendy microwave-assisted method. ten new n-alkyl (c6,8,10,12,14)-substituted azachalconium bromides (3a-e, 4a-e) were prepared from compounds 1 and 2 with corresponding alkyl halides. the antimicrobial activities of all the compounds were tested against enterococcus faecalis, yersinia pseudotuberculosis, pseudomonas aeruginosa, escherichia coli and staphylococcus aureus micro-organisms. | 2014 | 24571646 |
| structure of the y. pseudotuberculosis adhesin invasine. | enteropathogenic yersinia expresses several invasins that are fundamental virulence factors required for adherence and colonization of tissues in the host. within the invasin-family of yersinia adhesins, to date only invasin has been extensively studied at both structural and functional levels. in this work, we structurally characterize the recently identified inverse autotransporter invasine from yersinia pseudotuberculosis (formerly invasind from yersinia pseudotuberculosis strain ip31758) tha ... | 2017 | 28370712 |
| a study on the efficacy of the recombinant yersinia adhesin a vaccine against yersiniosis in the early phase. | yersinia pseudotuberculosis (y. ptb) is a zoonotic pathogenic bacterial species of the family enterobacteriaceae and causes yersiniosis, an acute intestinal infection in humans and animals. y. ptb is often implicated in lethal epidemics in zoo animals and reductions in the breeding population, but a valid prevention method has not been established. therefore, this study aimed to develop a vaccine for yersiniosis control. the immunogenicity of one of the adhesion factors involved in pathogenic me ... | 2017 | 28320976 |
| yptb3816 of yersinia pseudotuberculosis strain ip32953 is a virulence-related metallo-oligopeptidase. | although bacterial peptidases are known to be produced by various microorganisms, including pathogenic bacteria, their role in bacterial physiology is not fully understood. in particular, oligopeptidases are thought to be mainly involved in degradation of short peptides e.g. leader peptides released during classical protein secretion pathways. the aim of this study was to investigate effects of inactivation of an oligopeptidase encoding gene opda gene of yersinia pseudotuberculosis on bacterial ... | 2016 | 27887582 |
| yersinia pseudotuberculosis blocks neutrophil degranulation. | neutrophils are essential components of immunity and are rapidly recruited to infected or injured tissue. upon their activation, neutrophils release granules to the cell's exterior, through a process called degranulation. these granules contain proteins with antimicrobial properties that help combat infection. the enteropathogenic bacterium yersinia pseudotuberculosis successfully persists as an extracellular bacterium during infection by virtue of its translocation of virulence effectors (yersi ... | 2016 | 27620724 |
| a newly identified glutaminase-free ʟ-asparaginase (ʟ-aspg86) from the marine bacterium mesoflavibacter zeaxanthinifaciens. | ʟ-asparaginase (e.c. 3.5.1.1) is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. we identified the lasparaginase gene (ʟ-aspg86) in the genus mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. following phylogenetic analysis, the deduced amino acid sequence of ʟ-aspg86 (ʟ-aspg86) was grouped as a type i asparaginase with respective homologs in escherichia coli and yersinia pseudotub ... | 2016 | 26975773 |
| recombinant phospholipase a1 of the outer membrane of psychrotrophic yersinia pseudotuberculosis: expression, purification, and characterization. | the plda gene encoding membrane-bound phospholipase a1 of yersinia pseudotuberculosis was cloned and expressed in escherichia coli cells. recombinant phospholipase a1 (rplda) was isolated from inclusion bodies dissolved in 8 m urea by two-stage chromatography (ion-exchange and gel-filtration chromatography) as an inactive monomer. the molecular mass of the rplda determined by maldi-tof ms was 31.7 ± 0.4 kda. the highly purified rplda was refolded by 10-fold dilution with buffer containing 10 mm ... | 2016 | 26885582 |
| single channel activity of ompf-like porin from yersinia pseudotuberculosis. | to gain a mechanistic insight in the functioning of the ompf-like porin from yersinia pseudotuberculosis (yompf), we compared the effect of ph variation on the ion channel activity of the protein in planar lipid bilayers and its binding to lipid membranes. the behavior of yompf channels upon acidification was similar to that previously described for escherichia coli ompf. in particular, a decrease in ph of the bathing solution resulted in a substantial reduction of yompf single channel conductan ... | 2016 | 26854962 |
| affinity chromatography of chaperones based on denatured proteins: analysis of cell lysates of different origin. | molecular chaperones are involved in folding, oligomerization, transport, and degradation of numerous cellular proteins. most of chaperones are heat-shock proteins (hsps). a number of diseases of various organisms are accompanied by changes in the structure and functional activity of chaperones, thereby revealing their vital importance. one of the fundamental properties of chaperones is their ability to bind polypeptides lacking a rigid spatial structure. here, we demonstrate that affinity chrom ... | 2016 | 26644295 |
| enteric pathogens deploy cell cycle inhibiting factors to block the bactericidal activity of perforin-2. | perforin-2 (mpeg1) is an effector of the innate immune system that limits the proliferation and spread of medically relevant gram-negative, -positive, and acid fast bacteria. we show here that a cullin-ring e3 ubiquitin ligase (crl) complex containing cullin-1 and βtrcp monoubiquitylates perforin-2 in response to pathogen associated molecular patterns such as lps. ubiquitylation triggers a rapid redistribution of perforin-2 and is essential for its bactericidal activity. enteric pathogens such a ... | 2015 | 26418746 |
| [bacterial recombinant l-asparaginases: properties, structure and anti-proliferative activity]. | for more than 40 years l-asparaginases are used in combined therapy of acute lymphoblastic leukemia in children and the range of tumors sensitive to these enzymes constantly extends. this review summarizes results of studies aimed at creation of new systems for heterological expression of bacterial l-asparaginases as erwinia carotovora (ewa), helicobacter pylori (hpa), yersinia pseudotuberculosis (ypa) and rhodospirillum rubrum (rra); special attention is paid to isolation of purified enzymes an ... | 2015 | 26215408 |
| the supramolecular structure of lps-chitosan complexes of varied composition in relation to their biological activity. | the complexes of chitosan (ch) with lipopolysaccharides (lpss) from escherichia coli o55:b5 (e-lps) and yersinia pseudotuberculosis 1b 598 (y-lps) of various weight compositions were investigated using quasi-elastic light scattering, ζ-potential distribution assay and atomic force microscopy. the alteration of ζ-potential of e-lps-ch complexes from negative to positive values depending on ch content was detected. the y-lps-ch complexes had similar positive ζ-potentials regardless of ch content. ... | 2015 | 25843841 |
| [cross-immunogenicity of various bacterial l-asparaginases]. | evaluate immune response in mice against various l-asparaginases and determine their cross-immunogenicity. | 2015 | 25816523 |
| adhesive properties of yapv and paralogous autotransporter proteins of yersinia pestis. | yersinia pestis is the causative agent of plague. this bacterium evolved from an ancestral enteroinvasive yersinia pseudotuberculosis strain by gene loss and acquisition of new genes, allowing it to use fleas as transmission vectors. infection frequently leads to a rapidly lethal outcome in humans, a variety of rodents, and cats. this study focuses on the y. pestis kim yapv gene and its product, recognized as an autotransporter protein by its typical sequence, outer membrane localization, and am ... | 2015 | 25690102 |
| the adhesive protein invasin of yersinia pseudotuberculosis induces neutrophil extracellular traps via β1 integrins. | yersinia pseudotuberculosis adhesive protein invasin is crucial for the bacteria to cross the intestine epithelium by binding to β1 integrins on m-cells and gaining access to the underlying tissues. after the crossing invasin can bind to β1 integrins on other cell surfaces, however effector proteins delivered by the type iii secretion system y. pseudotuberculosis efficiently inhibit potential immune responses induced by this interaction. here, we use mutant y. pseudotuberculosis strains lacking ... | 2015 | 25576025 |
| lcrv delivered via type iii secretion system of live attenuated yersinia pseudotuberculosis enhances immunogenicity against pneumonic plague. | here, we constructed a yersinia pseudotuberculosis mutant strain with arabinose-dependent regulated and delayed shutoff of crp expression (arac p(bad) crp) and replacement of the msbb gene with the escherichia coli msbb gene to attenuate it. then, we inserted the asd mutation into this construction to form χ10057 [δasd-206 δmsbb868::p(msbb) msbb(ec) δp(crp21)::tt arac p(bad) crp] for use with a balanced-lethal asd-positive (asd(+)) plasmid to facilitate antigen synthesis. a hybrid protein compos ... | 2014 | 25114109 |
| study of effect of substitution of the penultimate amino acid residue on expression, structure, and functional properties of yersinia pseudotuberculosis ompy porin. | the purpose of the study was to compare the expression of two yersinia pseudotuberculosis proteins, wild-type porin ompy and the mutant porin ompy designated as ompy-q having the uncharged amino acid residue gln instead of positively charged arg at the penultimate position in the same heterologous host. according to the literature, a similar substitution (lys to gln) of the penultimate amino acid residue in neisseria meningitidis porin pora drastically improved the assembly of the protein in the ... | 2014 | 25108332 |
| rabbit monoclonal antibodies directed at the t3ss effector protein yopm identify human pathogenic yersinia isolates. | the yersinia outer protein m (yopm) is a type 3 secretion system (t3ss)-dependent effector protein of yersinia enterocolitica, yersinia pseudotuberculosis and yersinia pestis. although yopm is indispensable for full virulence, its molecular functions still remain largely elusive. recently, we could identify the recombinant yopm (ryopm) protein derived from the y. enterocolitica strain 8081 (jb580) as a cell-penetrating protein, which down-regulates the expression of various pro-inflammatory cyto ... | 2014 | 24636859 |
| mutant ompf porins of yersinia pseudotuberculosis with deletions of external loops: structure-functional and immunochemical properties. | recombinant mutant ompf porins from yersinia pseudotuberculosis outer membrane were obtained using site-directed mutagenesis. here we used four ompf mutants where single extracellular loops l1, l4, l6, and l8 were deleted one at a time. the proteins were expressed in escherichia coli at levels comparable to full-sized recombinant ompf porin and isolated from the inclusion bodies. purified trimers of the mutant porins were obtained after dialysis and consequent ion-exchange chromatography. change ... | 2014 | 24530904 |
| bacteria induce prolonged pmn survival via a phosphatidylcholine-specific phospholipase c- and protein kinase c-dependent mechanism. | polymorphonuclear leukocytes (pmns) are essential for the human innate immune defense, limiting expansion of invading microorganisms. pmn turnover is controlled by apoptosis, but the regulating signaling pathways remain elusive, largely due to inherent differences between mice and humans that undermine use of mouse models for understanding human pmn biology. here, we aim to elucidate signal transduction mediating survival of human peripheral blood pmns in response to bacteria, such as yersinia p ... | 2014 | 24498214 |
| the wzy o-antigen polymerase of yersinia pseudotuberculosis o:2a has a dependence on the wzz chain-length determinant for efficient polymerization. | lipopolysaccharide is a major immunogenic structure for the pathogen yersinia pseudotuberculosis, which contains the o-specific polysaccharide (ops) that is presented on the cell surface. the ops contains many repeats of the oligosaccharide o-unit and exhibits a preferred modal chain length that has been shown to be crucial for cell protection in yersinia. it is well established that the wzz protein determines the preferred chain length of the ops, and in its absence, the polymerization of o uni ... | 2013 | 24164168 |
| microbiological quality of fresh-cut carrots and process waters. | fresh vegetables may be contaminated by pathogens in different ways after harvest. pathogenic microorganisms associated with fresh vegetables can cause severe outbreaks of foodborne disease. we discuss here the results of microbiological analysis of carrot samples, as well as of washing, processing, and wastewater samples. washed, unpeeled carrots generally contained the highest aerobic plate counts (mean, 5.5 log cfu/g). escherichia coli was not detected in any carrot or water sample examined. ... | 2013 | 23834799 |
| patterning bacterial communities on epithelial cells. | micropatterning of bacteria using aqueous two phase system (atps) enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. this method was used to compare the effects of escherichia coli strain mg1655 and an isogenic invasive counterpart that expresses the invasin (inv) gene from yersinia pseudotuberculosis on the underlying epithelial cell layer. large portions of the cell layer beneath the invasive strain were killed or detached ... | 2013 | 23785519 |
| human single-chain urokinase is activated by the omptins pgte of salmonella enterica and pla of yersinia pestis despite mutations of active site residues. | fibrinolysis is important in cell migration and tightly regulated by specific inhibitors and activators; of the latter, urokinase (upa) associates with enhancement of cell migration. active upa is formed through cleavage of the single-chain upa (scupa). the salmonella enterica strain 14028r cleaved human scupa at the peptide bond lys158-ile159, the site cleaved also by the physiological activator human plasmin. the cleavage led to activation of scupa, while no cleavage or activation were detecte ... | 2013 | 23763588 |
| [physicochemical properties and antiproliferative activity of recombinant yersinia pseudotuberculosis l-asparaginase]. | the physicochemical, catalytic, and antiproliferative activity of a recombinant l-asparaginase from yersinia pseudotuberculosis (ypa) have been studied. the following results were obtained: the k(m) value for l-asparagine is 17 +/- 0.9 microm, the optimal temperature is 60 degrees c, ph is 8.0, pi is 5.4 +/- 0.3, the l-glutaminase activity is no more than 5-6% of the l-asparaginase activity, and the antiproliferative activity on the fisher l5178y lymphadenosis cell line comprised t/c = 136% (p < ... | 2013 | 23662446 |
| molecular cloning, isolation, and properties of chaperone skp from yersinia pseudotuberculosis. | the skp gene of yersinia pseudotuberculosis was expressed without its signal sequence in escherichia coli bl21(de3) cells. the recombinant protein skp accumulated in soluble form in the cytoplasm of the producer strain. the protein was isolated and characterized: the molecular weight, isoelectric point, n-terminal amino acid sequence (20 amino acid residues), and the content of the secondary structure elements were determined. using cross-linking stabilization and high-mass maldi-tof mass spectr ... | 2012 | 23240570 |
| the yersinia pseudotuberculosis degradosome is required for oxidative stress, while its pnpase subunit plays a degradosome-independent role in cold growth. | yersinia polynucleotide phosphorylase (pnpase), a 3'-5' exoribonuclease, has been shown to affect growth during several stress responses. in escherichia coli, pnpase is one of the subunits of a multiprotein complex known as the degradosome, but also has degradosome-independent functions. the carboxy-terminus of e. coli ribonuclease e (rnase e) serves as the scaffold upon which pnpase, enolase (a glycolytic enzyme), and rhlb helicase all have been shown to bind. in the yersiniae, only pnpase has ... | 2012 | 23082859 |
| the yersinia pseudotuberculosis outer membrane protein ail recruits the human complement regulatory protein factor h. | previous investigations characterizing the mechanism(s) of complement resistance in yersinia pseudotuberculosis showed that the outer membrane protein ail can functionally recruit the regulator of the classical and lectin pathways of complement, c4b-binding protein. in this study, we extend these observations and show that ail can also recruit the regulator of the alternative pathway (ap), factor h (fh). binding to fh was dependent on ail expression and observed in the context of full-length lps ... | 2012 | 22956584 |
| [yersinia pseudotuberculosis mutant ompf porins with deletions of the external loops: genetic constructions design, expression, isolation and refolding]. | yersinia pseudotuberculosis outer membrane (om) recombinant mutant ompf porins with deletions of the external loops l1, l6 and l8 were obtained using site-directed mutagenesis of the recombinant plasmid including ompf gene. heterologeous expression of the mutant proteins was carried out in strain rosetta of escherichia coli (novagen, usa), porins with the deletions were isolated from the inclusion bodies. mutant proteins in oligomeric form were obtained as result of dialysis and ion-exchange chr ... | 2012 | 22792719 |
| bacteriophages capable of lysing yersinia pestis and yersinia pseudotuberculosis: efficiency of plating tests and identification of receptors in escherichia coli k-12. | | 2012 | 22782755 |
| fdec, a novel broadly conserved escherichia coli adhesin eliciting protection against urinary tract infections. | the increasing antibiotic resistance of pathogenic escherichia coli species and the absence of a pan-protective vaccine pose major health concerns. we recently identified, by subtractive reverse vaccinology, nine escherichia coli antigens that protect mice from sepsis. in this study, we characterized one of them, ecok1_0290, named fdec (factor adherence e. coli) for its ability to mediate e. coli adhesion to mammalian cells and extracellular matrix. this adhesive propensity was consistent with t ... | 2012 | 22496310 |
| functional recruitment of the human complement inhibitor c4bp to yersinia pseudotuberculosis outer membrane protein ail. | ail is a 17-kda chromosomally encoded outer membrane protein that mediates serum resistance (complement resistance) in the pathogenic yersiniae (yersinia pestis, y. enterocolitica, and y. pseudotuberculosis). in this article, we demonstrate that y. pseudotuberculosis ail from strains pb1, 2812/79, and ypiii/pib1 (serotypes o:1a, o:1b, and o:3, respectively) can bind the inhibitor of the classical and lectin pathways of complement, c4b-binding protein (c4bp). binding was observed irrespective of ... | 2012 | 22467648 |
| a natural system of chromosome transfer in yersinia pseudotuberculosis. | the high pathogenicity island of yersinia pseudotuberculosis ip32637 was previously shown to be horizontally transferable as part of a large chromosomal segment. we demonstrate here that at low temperature other chromosomal loci, as well as a non-mobilizable plasmid (puc4k), are also transferable. this transfer, designated gdt4 (generalized dna transfer at 4°c), required the presence of an ip32637 endogenous plasmid (pgdt4) that carries several mobile genetic elements and a conjugation machinery ... | 2012 | 22412380 |
| toxin-induced rhoa activity mediates ccl1-triggered signal transducers and activators of transcription protein signaling. | rhoa is reportedly involved in signal transducers and activators of transcription (stat)-dependent transcription. however, the pathway connecting the gtpase and stat signaling has not been characterized. here, we made use of bacterial toxins, which directly activate rho gtpases to analyze this pathway. cytotoxic necrotizing factors (cnfs) are produced by pathogenic escherichia coli strains and by yersinia pseudotuberculosis. they activate small gtpases of the rho family by deamidation of a gluta ... | 2012 | 22311973 |
| manipulation of host membranes by the bacterial pathogens listeria, francisella, shigella and yersinia. | bacterial pathogens display an impressive arsenal of molecular mechanisms that allow survival in diverse host niches. subversion of plasma membrane and cytoskeletal functions are common themes associated to infection by both extracellular and intracellular pathogens. moreover, intracellular pathogens modify the structure/stability of their membrane-bound compartments and escape degradation from phagocytic or autophagic pathways. here, we review the manipulation of host membranes by listeria mono ... | 2016 | 27448494 |
| surface display of n-terminally anchored invasin by lactobacillus plantarum activates nf-κb in monocytes. | the probiotic lactic acid bacterium lactobacillus plantarum is a potential delivery vehicle for mucosal vaccines because of its generally regarded as safe (gras) status and ability to persist at the mucosal surfaces of the human intestine. however, the inherent immunogenicity of vaccine antigens is in many cases insufficient to elicit an efficient immune response, implying that additional adjuvants are needed to enhance the antigen immunogenicity. the goal of the present study was to increase th ... | 2012 | 22706054 |
| characterization of the protective immune response to yersinia pseudotuberculosis infection in mice vaccinated with an lcrv-secreting strain of lactococcus lactis. | pseudotuberculosis is an infection caused by the bacterial enteropathogen yersinia pseudotuberculosis and is considered to be a significant problem in veterinary medicine. we previously found that intranasal administration of a recombinant lactococcus lactis strain that secretes the low-calcium response v (lcrv) antigen from y. pseudotuberculosis (ll-lcrv) confers protection against a lethal y. pseudotuberculosis infection. here, we aimed at characterizing the immunological basis of this lcrv-el ... | 2016 | 27742220 |
| yope specific cd8+ t cells provide protection against systemic and mucosal yersinia pseudotuberculosis infection. | prior studies indicated that cd8+ t cells responding to a surrogate single antigen expressed by y. pseudotuberculosis, ovalbumin, were insufficient to protect against yersiniosis. herein we tested the hypothesis that cd8+ t cells reactive to the natural yersinia antigen yope would be more effective at providing mucosal protection. we first confirmed that immunization with the attenuated ksga- strain of y. pseudotuberculosis generated yope-specific cd8+ t cells. these t cells were protective agai ... | 2017 | 28207901 |
| cd8(+) t cells specific to a single yersinia pseudotuberculosis epitope restrict bacterial replication in the liver but fail to provide sterilizing immunity. | cd8(+) t cells use contact-dependent cytolysis of target cells to protect the host against intracellular pathogens. we have previously shown that cd8(+) t cells and perforin are required to protect against the extracellular pathogen yersinia pseudotuberculosis. here we establish an experimental system where cd8(+) t cells specific to a single model antigen are the only memory response present at time of challenge. using mice immunized with a vaccine strain of listeria monocytogenes that expresse ... | 2016 | 27268148 |
| [sanitary significance of soil sea coasts]. | there was investigated the dynamics of growth of listeria monocytogenes and yersinia pseudotuberculosis in soils of sea coast (mid-flight and maritime soils). these bacteria were shown to reproduced well in all researched soils, preferring nevertheless maritime soils. the content of the humus was determined to be the one of the limiting factors restricting the multiplication of pathogenic bacteria in studied soils. abiotic characteristic of soils of sea coast were established to render the direc ... | 2015 | 26625609 |
| [the metabolism of the innate immunity cells in bacterial infections]. | metabolic activity of innate immunity cells infected by various doses of gram-negative (yersinia pseudotuberculosis, salmonella enteritidis) and gram-positive (staphylococcus aureus, listeria monocytogenes) bacteria has been investigated. using various animal models we found that during the initial period (up to 2 days) changes of infection in cellular responses depend on the type of the pathogen. in response to infection caused by gram-negative bacteria predominant neutrophil accumulation in th ... | 2015 | 25762604 |
| [effect of volatile metabolites from germinating seeds on the reproduction of the bacteria listeria monocytogenes and yersinia pseudotuberculosis]. | the biological activity of volatile metabolites of germinating seeds of cabbage (brassica oleacia), carrot (daukus carota), salad (lactuca sativa), and corn (zea mays l.) against listeria monocytogenes and yersinia pseudotuberculosis was studied. it was shown that volatile metabolites are transfer factors and can be the sole carbon and energy source for these bacteria. methanol is the main substance affecting their growth and reproduction. | 2015 | 22834302 |
| piericidin a1 blocks yersinia ysc type iii secretion system needle assembly. | the type iii secretion system (t3ss) is a bacterial virulence factor expressed by dozens of gram-negative pathogens but largely absent from commensals. the t3ss is an attractive target for antimicrobial agents that may disarm pathogenic bacteria while leaving commensal populations intact. we previously identified piericidin a1 as an inhibitor of the ysc t3ss in yersinia pseudotuberculosis. piericidins were first discovered as inhibitors of complex i of the electron transport chain in mitochondri ... | 2017 | 28217742 |
| bacterial internalization is required to trigger nik-dependent nf-κb activation in response to the bacterial type three secretion system. | infection of human cells with yersinia pseudotuberculosis expressing a functional type iii secretion system (t3ss) leads to activation of host nf-κb. we show that the yersinia t3ss activates distinct nf-κb pathways dependent upon bacterial subcellular localization. we found that wildtype yersinia able to remain extracellular triggered nf-κb activation independently of the non-canonical nf-κb kinase nik in hek293t cells. in contrast, yersinia lacking the actin-targeting effectors yopeho, which be ... | 2017 | 28166267 |
| inhibition of the injectisome and flagellar type iii secretion systems by inp1855 impairs pseudomonas aeruginosa pathogenicity and inflammasome activation. | with the rise of multidrug resistance, pseudomonas aeruginosa infections require alternative therapeutics. the injectisome (it3ss) and flagellar (ft3ss) type iii secretion systems are 2 virulence factors associated with poor clinical outcomes. it3ss translocates toxins, rod, needle, or regulator proteins, and flagellin into the host cell cytoplasm and causes cytotoxicity and nlrc4-dependent inflammasome activation, which induces interleukin 1β (il-1β) release and reduces interleukin 17 (il-17) p ... | 2016 | 27412581 |
| spectroscopic studies on hasa from yersinia pseudotuberculosis. | heme acquisition system a (hasa) is known as a hemophore in gram-negative pathogens. the ferric heme iron is coordinated by tyr-75 and his-32 in holo-hasa from pseudomonas aeruginosa (hasapa). in contrast, in holo-hasa from yersinia pseudotuberculosis (hasayp), our spectroscopic studies suggest that only tyr-75 coordinates to the ferric heme iron. the substitution of gln-32 with alanine in hasayp does not alter the spectroscopic properties, indicating that gln-32 is not an axial ligand for the h ... | 2014 | 24857805 |
| a type iii secretion system inhibitor targets yopd while revealing differential regulation of secretion in calcium-blind mutants of yersinia pestis. | numerous gram-negative pathogens rely upon type iii secretion (t3s) systems to cause disease. several small-molecule inhibitors of the type iii secretion systems have been identified; however, few targets of these inhibitors have been elucidated. here we report that 2,2'-thiobis-(4-methylphenol) (compound d), inhibits type iii secretion in yersinia pestis, yersinia pseudotuberculosis, and pseudomonas aeruginosa. yopd, a protein involved in the formation of the translocon and regulatory processes ... | 2014 | 24247143 |
| pseudomonas fluorescens alters the intestinal barrier function by modulating il-1β expression through hematopoietic nod2 signaling. | ileal crohn's disease is related to nod2 mutations and to a gut barrier dysfunction. pseudomonas fluorescens has also been associated with ileal crohn's disease. the aim of this study was to determine the impact of p. fluorescens on the paracellular permeability in ileum and peyer's patches. | 2015 | 25659087 |
| surface-modified yeast cells: a novel eukaryotic carrier for oral application. | the effective targeting and subsequent binding of particulate carriers to m cells in peyer's patches of the gut is a prerequisite for the development of oral delivery systems. we have established a novel carrier system based on cell surface expression of the β1-integrin binding domain of invasins derived from yersinia enterocolitica and yersinia pseudotuberculosis on the yeast saccharomyces cerevisiae. all invasin derivatives were shown to be effectively expressed on the cell surface and recombi ... | 2016 | 26763373 |
| presence of salmonella spp., yersinia enterocolitica, yersinia pseudotuberculosis and escherichia coli o157:h7 in wild boars. | the european wild boar populations are growing and spreading to new areas, which might constitute a threat to public health, since wild boar can harbour pathogens with the potential to cause serious illness in humans. tonsils, ileocaecal lymph nodes and faecal samples were collected from 88 swedish wild boars and analysed for the presence of the zoonotic pathogens salmonella spp., yersinia enterocolitica, y. pseudotuberculosis and enterohaemorrhagic escherichia coli o157:h7 (ehec). a combination ... | 2014 | 24512817 |
| yersinia spp. in wild rodents and shrews in finland. | yersinia enterocolitica and yersinia pseudotuberculosis are important zoonotic bacteria causing human enteric yersiniosis commonly reported in europe. all y. pseudotuberculosis strains are considered pathogenic, while y. enterocolitica include both pathogenic and nonpathogenic strains which can be divided into six biotypes (1a, 1b, and 2-5) and about 30 serotypes. the most common types causing yersiniosis in europe are y. enterocolitica bioserotypes 4/o:3 and 2/o:9. strains belonging to biotype ... | 2017 | 28332937 |
| green fluorescent protein labeling of food pathogens yersinia enterocolitica and yersinia pseudotuberculosis. | labeling of bacteria with marker genes, such as green fluorescent protein, is a useful and practicable tool for tracking and enumerating bacterial cells in a complex environment e.g. discrimination from the indigenous background population. in this study, novel turbogfp prokaryotic expression vector was utilized for labeling of yersinia species. y. enterocolitica biovar 1a, biovar 2, biovar 4 and y. pseudotuberculosis were successfully transformed with the vector and expressed bright green fluor ... | 2017 | 27838541 |
| detection of pathogenic yersinia enterocolitica in pet djungarian hamsters in japan. | the prevalence of yersinia enterocolitica (y. enterocolitica) and yersinia pseudotuberculosis was examined in 151 pet animals including 108 rodents, 39 rabbits and four sugar gliders from 13 pet stores in the yamaguchi prefecture, japan. y. enterocolitica serogroup o:3 biotype 3 negative for the voges-proskauer reaction (o:3/3 variant vp-) was isolated from five djungarian hamsters (phodopus sungorus) raised at the same pet store. these pathogenic y. enterocolitica isolates carried the virulence ... | 2016 | 27396397 |
| detection, seroprevalence and antimicrobial resistance of yersinia enterocolitica and yersinia pseudotuberculosis in pig tonsils in northern italy. | yersiniosis is the third most common reported zoonoses in europe, with y. enterocolitica and y. pseudotuberculosis responsible for 98.66% and 0.94% of the confirmed human cases in 2013. from june 2013 to october 2014, 201 pigs at slaughter belonging to 67 batches were tested for y. enterocolitica and y. pseudotuberculosis in tonsils. diaphragm muscle samples were tested for antibodies against yersinia by a commercially available elisa test. y. enterocolitica 4/o:3 was detected in 55/201 pig tons ... | 2016 | 27500659 |
| [detection of yersinia enterocolitica bacteriophage phiye-f10 lysis spectrum and analysis of the relationship between lysis ability and virulence gene of yersinia enterocolitica]. | to determine the lysis spectrum of yersinia enterocolitica bacteriophage phiye-f10 and to analyze the relationship between the lysis ability of phiye-f10 and the virulence gene of yersinia enterocolitica. to observe the lysis ability of the phage phiye-f10 to the different yersinia strains with the double-layer technique. the strains used in this study including 213 of yersinia enterocolitica and 36 of yersinia pseudotuberculosis and 1 of yersinia pestis. the virulence genes of these yersinia en ... | 2016 | 27396162 |
| yersinia virulence factors - a sophisticated arsenal for combating host defences. | the human pathogens yersinia pseudotuberculosis and yersinia enterocolitica cause enterocolitis, while yersinia pestis is responsible for pneumonic, bubonic, and septicaemic plague. all three share an infection strategy that relies on a virulence factor arsenal to enable them to enter, adhere to, and colonise the host while evading host defences to avoid untimely clearance. their arsenal includes a number of adhesins that allow the invading pathogens to establish a foothold in the host and to ad ... | 2016 | 27347390 |
| yersiniosis in france: overview and potential sources of infection. | the aim of this study was to exploit the extensive database on strains of yersinia collected over more than 50 years in france in order to gain an overview of yersiniosis and potential sources of contamination in this country. | 2016 | 26987478 |
| 'add, stir and reduce': yersinia spp. as model bacteria for pathogen evolution. | pathogenic species in the yersinia genus have historically been targets for research aimed at understanding how bacteria evolve into mammalian pathogens. the advent of large-scale population genomic studies has greatly accelerated the progress in this field, and yersinia pestis, yersinia pseudotuberculosis and yersinia enterocolitica have once again acted as model organisms to help shape our understanding of the evolutionary processes involved in pathogenesis. in this review, we highlight the ge ... | 2016 | 26876035 |
| comparative genomic hybridization analysis of yersinia enterocolitica and yersinia pseudotuberculosis identifies genetic traits to elucidate their different ecologies. | enteropathogenic yersinia enterocolitica and yersinia pseudotuberculosis are both etiological agents for intestinal infection known as yersiniosis, but their epidemiology and ecology bear many differences. swine are the only known reservoir for y. enterocolitica 4/o:3 strains, which are the most common cause of human disease, while y. pseudotuberculosis has been isolated from a variety of sources, including vegetables and wild animals. infections caused by y. enterocolitica mainly originate from ... | 2015 | 26605338 |
| homology analysis and cross-immunogenicity of ompa from pathogenic yersinia enterocolitica, yersinia pseudotuberculosis and yersinia pestis. | the outer membrane protein a (ompa) is one of the intra-species conserved proteins with immunogenicity widely found in the family of enterobacteriaceae. here we first confirmed ompa is conserved in the three pathogenic yersinia: yersinia pestis, yersinia pseudotuberculosis and pathogenic yersinia enterocolitica, with high homology at the nucleotide level and at the amino acid sequence level. the identity of ompa sequences for 262 y. pestis strains, 134 y. pseudotuberculosis strains and 219 patho ... | 2015 | 26435220 |