| penetration of immunoglobulins through the klebsiella capsule and their effect on cell-surface hydrophobicity. | the ability of antibodies to cell-surface components of klebsiella to increase surface hydrophobicity and to gain access to antigens potentially masked by the capsule was investigated. treatment of capsulate or non-capsulate strains with the respective autologous antiserum resulted in a marked increase in surface hydrophobicity. antisera raised against a rough non-capsulate (k-o-) strain had little effect on the surface hydrophobicity of either of the capsulate strains k1+o1+ and k2+o1+, or of t ... | 1988 | 3286873 |
| [clinical evaluation of ceftriaxone in the treatment of neonatal infections]. | ceftriaxone (ctrx) was administered to the newborn and its clinical effectiveness as well as its blood and cerebrospinal fluid levels were studied. 1. average blood levels of ctrx 1 hour after single intravenous administration were 39 micrograms/ml in 2 cases receiving about 10 mg/kg, 70 micrograms/ml in 2 other cases receiving 20 mg/kg and 208 micrograms/ml in one receiving 52.6 mg/kg. as is apparent from these cases data, blood levels of ctrx were dose dependent. blood levels of the drug were ... | 1988 | 3286924 |
| [cold agglutinin disease]. | cold agglutinin disease is a normo- or macrocytic anemia due to antibodies, active under body temperature, mostly belonging to the immunoglobulin class m. initially the agglutination of erythrocytes with acrocyanosis is reversible at body temperature. high antibody activity or long lasting period of coldness lead to intravascular or intrahepatic hemolysis, but high risk anemia is rare. beside the idiopathic form, infection induced (especially infectious mononucleosis, cytomegalovirus, mycoplasma ... | 1988 | 3286990 |
| nature of monocyclic beta-lactam antibiotic nocardicin a to beta-lactamases. | nocardicin a is the antibiotic which was first found to possess a monocyclic beta-lactam ring. this antibiotic was inactivated by the cleavage of its beta-lactam ring. the direct spectrophotometric assay was applied to measure the rate of enzymatic hydrolysis of nocardicin a. nocardicin a was highly stable to both chromosomal and plasmid-mediated beta-lactamases. of the nine beta-lactam antibiotics including cefoxitin and cefuroxime, nocardicin a was the most stable to the beta-lactamases tested ... | 1988 | 3287105 |
| in vitro hexagonal assembly of r-form lipopolysaccharides: effect of ph on the mg+2-mediated hexagonal assembly. | the r-form lipopolysaccharide (lps) from escherichia coli k-12, from which cationic material had been removed by electrodialysis and the ph of which had fallen to 3.6, formed a rough hexagonal lattice structure with the lattice constant of about 19 nm. the rough hexagonal structure was maintained in buffers at ph 5 or lower but disintegrated into the ribbon-like structures in buffers at ph 6 or higher. however, in the presence of 10 mm mg2+, the hexagonal lattice structure was not disintegrated ... | 1988 | 3287106 |
| the effect on the function of the transcriptional activator ntrc from klebsiella pneumoniae of mutations in the dna-recognition helix. | we have constructed mutations in what we predict to be the dna-recognition helix of klebsiella pneumoniae ntrc, which regulates transcription from promoters under global nitrogen control. mutations which disrupt the helix lead to complete loss of function. all point mutants tested were able to activate transcription from the sigma 54-dependent glna promoter, but only those retaining some ability to recognise ntrc binding sites, as evidenced by their ability to repress the ntrb promoter and the u ... | 1988 | 3287338 |
| [klebsiella pneumoniae as the cause of arthritis of the knee]. | | 1988 | 3287728 |
| microbiology and pharmacology of aztreonam. | aztreonam, the first monobactam antibiotic, represents a significant evolutionary advance in antimicrobial therapy. aztreonam is stable in the presence of the hydrolytic beta-lactamase enzymes; as such, it is effective against most gram-negative aerobes, including pseudomonas aeruginosa, escherichia coli, klebsiella, and enterobacter. due to its specific spectrum of activity, aztreonam does not disturb the normal gram-positive and anaerobic intestinal flora. the safety profile of aztreonam is su ... | 1988 | 3287750 |
| nose-only versus whole-body aerosol exposure for induction of upper respiratory infections of laboratory mice. | the effectiveness of two aerosol delivery systems, nose-only and whole-body, were compared using swiss-webster mice and two pathogens, klebsiella pneumoniae and venezuelan equine encephalitis (vee) virus. with k. pneumoniae the median lethal dose (ld50) and the mean time to death correlated with the inhaled dose. an ld50 value of 335 colony forming units (cfu) for nose-only exposure was significantly less than the ld50 value of 3741 cfu obtained for whole-body exposure. the ld50 values obtained ... | 1988 | 3287878 |
| infection in a patient with polycystic kidney and liver disease: noninvasive localization and treatment. | | 1988 | 3287915 |
| risk factors associated with a cluster of urinary tract infections in a geriatric unit caused by klebsiella pneumoniae resistant to multiple antibiotics. | | 1988 | 3288014 |
| effects of bacterial endotoxin on ciliary activity in the tubotympanum. | the discovery of endotoxin in effusion of otitis media with effusion (ome) has suggested the possibility that bacterial endotoxin may be involved in the pathogenesis and development of ome. in this study, we investigated the direct effects of lipopolysaccharide (lps), possessing the major part of endotoxin activity, on the ciliary activity in the tubotympanum. the study shows that lps deteriorates ciliary activity in a dose-response fashion and that even low levels can, with extended exposure, c ... | 1988 | 3288076 |
| factors promoting survival of bacteria in chlorinated water supplies. | results of our experiments showed that the attachment of bacteria to surfaces provided the greatest increase in disinfection resistance. attachment of unencapsulated klebsiella pneumoniae grown in medium with high levels of nutrients to glass microscope slides afforded the microorganisms as much as a 150-fold increase in disinfection resistance. other mechanisms which increased disinfection resistance included the age of the biofilm, bacterial encapsulation, and previous growth conditions (e.g., ... | 1988 | 3288119 |
| the use of bacteriophage-mediated depolymerisation in the structural investigation of the capsular polysaccharide from escherichia coli serotype k36. | the structure of the repeating unit of the capsular polysaccharide from escherichia coli serotype k36 has been established from the results of spectroscopic and chemical analyses of (a) p1, the tetrasaccharide obtained on depolymerisation of the polysaccharide with a bacteriophage-borne endo-galactosidase, (b) p1-alditol, and (c) the original polysaccharide. the repeating unit, which is identical to that reported for klebsiella k57, has the following structure. (formula: see text). | 1988 | 3288342 |
| analysis by the reductive-cleavage method of linkage positions in a polysaccharide containing 4-linked d-glucopyranosyluronic residues. | the fate of 4-linked d-glucopyranosyluronic residues under reductive-cleavage conditions was investigated by using the klebsiella aerogenes type 54 strain a3 capsular polysaccharide. treatment of the fully methylated polysaccharide with triethylsilane and trimethylsilyl trifluoromethanesulfonate in dichloromethane, followed by in situ acetylation, yielded 1,5-anhydro-2,3,4,6-tetra-o-methyl-d-glucitol, 3,4-di-o-acetyl-1,5-anhydro-2,6-di-o-methyl-d-glucitol, and 3-o-acetyl-1,5-anhydro-2,4-di-o-met ... | 1988 | 3288343 |
| carumonam's in-vitro activity against gram-negative bacteria and its stability to their beta-lactamases. | the in vitro activity of the novel monobactam carumonam (ro17-2301) was evaluated on 311 gram-negative clinical isolates in comparison to aztreonam, cefotaxime, ceftazidime, cefotetan and ceftriaxone. carumonam showed an antibacterial potency equal to or higher than any other reference compound; in particular it was the most effective against proteus indole positive and klebsiella sp. its antipseudomonal activity was comparable to that of ceftazidime and it showed, together with aztreonam, the h ... | 1988 | 3288365 |
| species-related differences in the incidence of gram-negative bacteria isolated from the cloaca of clinically normal psittacine birds. | cloacal swabs from 506 clinically normal psittacine birds of 22 species were aerobically cultured for bacteria and yeasts. in 45 (9%) samples, no microbial organisms were recovered. gram-positive bacteria were recovered from 474 (91%) samples. the incidences of gram-negative bacteria and yeasts were: escherichia coli 157 (31%), enterobacter sp. 21 (4%), klebsiella sp. 3 (0.6%), pseudomonas sp. 4 (0.8%), and yeasts 26 (5%). differences were noted in the recovery rate of e. coli among the various ... | 1988 | 3289533 |
| interaction between human natural anti-alpha-galactosyl immunoglobulin g and bacteria of the human flora. | anti-alpha-galactosyl immunoglobulin g (anti-gal) is a natural antibody present in unusually high amounts in human sera. it constitutes as much as 1% of circulating immunoglobulin g in humans and displays a distinct specificity for the carbohydrate epitope galactosyl alpha(1----3) galactosyl (gal alpha 1----3gal). recently, it has been suggested by various investigators that anti-gal may be related to some autoimmune phenomena, since marked elevation of its titer was found in sera of patients wi ... | 1988 | 3290105 |
| microbiological and clinical aspects of aspiration pneumonia. | aspiration pneumonia is characterized by a pneumonitis in a dependent segment of the lung with typical necrosis or abscess-formation in the parenchyma. observed aspiration or predisposition to aspiration, cavitation or abscess formation, with or without empyema fluid and isolation of distinctive micro-organisms are important clues to the diagnosis. diagnostic procedures to collect anaerobic uncontaminated secretions are transtracheal aspiration, blood cultures, pleural fluid aspiration, fibreopt ... | 1988 | 3290185 |
| evaluation of use of signal system of blood culture in paediatrics. | a new method of blood culture, the signal system (oxoid), was assessed in paediatric practice. isolation rates of pathogens, frequency of contamination (false positive results), and time taken to detect positive cultures were analysed. four hundred and seventy nine organisms were isolated from 457 of 3000 cultures collected, of which 283 organisms were considered to be clinically important. the overall rate of positive cultures was 15.2%, and clinically important organisms were isolated from 9.1 ... | 1988 | 3290274 |
| effects of cell recycle on microbial cell viability and metabolism. | | 1988 | 3290416 |
| presence of less heavily encapsulated klebsiella pneumoniae capsular type 1 in semen of healthy stallions and cervical swabs of mares suffering from metritis and comparison of virulence between heavily and less heavily encapsulated strains. | | 1988 | 3290560 |
| nucleotide sequence of the nife and nifn genes from klebsiella pneumoniae. | | 1988 | 3290850 |
| [effect of a purified glycoprotein substance extracted from klebsiella pneumoniae in the clinical course of acute infections of the airways in patients with chronic bronchitis. italian group for the study of biological immunomodulators]. | | 1988 | 3291026 |
| [reduction using an immunomodulator of the level of respiratory infections in chronic bronchitis]. | fifty-six patients with recurrent bronchopulmonary infections associated with chronic bronchitis were randomly allocated to 2 groups, to assess whether treatment with an immunomodulator, diribiotin ck, could enhance resistance to infection. the double-blind, placebo-controlled prospective trial over a 9 month period showed that this immunomodulator was well tolerated and significantly reduced the rate of respiratory tract infection. the drug also significantly reduced the prescribing of antibiot ... | 1988 | 3291072 |
| ct findings in emphysematous cystitis. | | 1988 | 3291372 |
| experience with antimicrobial combinations in urinary tract infections. | four antimicrobial combinations were tried in the management of recurrent upper urinary tract infections: (i) rifampicin + trimethoprim (50 patients); (ii) amoxycillin + clavulanic acid (50 patients); (iii) ampicillin + sulbactam (10 patients); and (iv) pivampicillin + pivmecillinam (50 patients). the initial and final microbiological success rate was excellent with all four drug combinations; all were well tolerated. although monotherapy should be the rule in antimicrobial chemotherapy, there a ... | 1988 | 3292180 |
| characteristics of cysteine-requiring strains of klebsiella isolated from urinary tract infections. | clinical and bacteriological findings in seven cases of urinary tract infection with cysteine-requiring strains of klebsiella are described. the organisms were isolated from patients with long-standing urinary tract abnormalities and grew as small (c. 1 mm) colonies on macconkey agar. the organisms failed to grow in a minimal medium supplemented with sodium sulphate but grew when the medium was supplemented with cysteine sulphinic acid, sodium sulphide or l-cysteine. the smallest amount of cyste ... | 1988 | 3292770 |
| klebsiella pneumoniae arthritis in a child. | | 1988 | 3293001 |
| bacterial translocation in dianhydrodulcitol-treated mice. | escherichia, proteus, klebsiella and streptococcus strains were isolated from mesenteric lymph nodes, spleens and livers of conventional mice treated with dianhydrodulcitol (dad), indicating that intestinal bacteria had appeared in organs usually containing no bacteria. the frequency of bacterial translocation showed direct relation to the dose of the drug and appeared simultaneously with the spleen atrophy caused by dad. | 1988 | 3293340 |
| production of a phosphatidylglycerol-like substance by genital flora bacteria. | bacteria contaminating amniotic fluid have anecdotally been shown to produce a phosphatidylglycerol-like substance, thus giving false positive tests for phosphatidylglycerol in vaginal pool fluid from patients with premature rupture of the membranes. isolates of escherichia coli, proteus mirabilis, acinetobacter calcoaceticus, klebsiella pneumoniae, staphylococcus aureus, coagulase-negative staphylococcus, streptococcus faecalis, and streptococcus agalactiae (group b streptococcus) were grown in ... | 1988 | 3293449 |
| novel plasmid-mediated beta-lactamase in clinical isolates of klebsiella pneumoniae more resistant to ceftazidime than to other broad-spectrum cephalosporins. | multiresistant klebsiella pneumoniae strains isolated from three patients in the same intensive care unit were more resistant to ceftazidime than to cefotaxime and aztreonam but remained susceptible to moxalactam and imipenem. resistance to beta-lactams, kanamycin, streptomycin, sulfonamides, and tetracyclines was transferable to escherichia coli by conjugation and was lost en bloc after treatment with ethidium bromide. agarose gel electrophoresis of wild types and transconjugants indicated that ... | 1988 | 3293523 |
| [non-clostridial gas-producing brain abscess in a brain death patient--report of a case]. | a 46 year old female was admitted to our emergency room because of cardiopulmonary arrest by hanging. after ten minutes cardiopulmonary resuscitation, she was resuscitated but her consciousness did'nt recover. ct-scans on the day of admission showed no abnormalities but on the second hospital day it showed symmetrical low density areas within the basal ganglia, the thalamus, the hippocampus, and the occipital lobe. there were marked enhanced effect in cerebral sulci due to cytotoxic edema of hyp ... | 1988 | 3293638 |
| comparative in vitro activity of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone against hospital strains of gram-negative and -positive bacteria. | the in vitro antibacterial activities of amoxycillin/clavulanate (augmentin), ceftazidime and ceftriaxone were compared against 330 gram-negative and gram-positive strains isolated from clinical specimens received at the king abdulaziz university hospital (kauh) in saudi arabia. the antibacterial susceptibility was determinated by stokes method and by the minimal inhibitory concentration (mic) using an agar dilution method. ceftazidime and ceftriaxone were the most active antibiotics, inhibiting ... | 1988 | 3293818 |
| the effect of chlorhexidine and benzydamine mouthwashes on mucositis induced by therapeutic irradiation. | a variety of mouthwashes are frequently used in the management of irradiation-induced mucositis. benzydamine has recently been introduced for alleviating this condition. its efficacy as a mouthwash was compared with chlorhexidine in two groups of patients receiving radiotherapy for oral carcinoma. mucositis and pain were recorded over a 6 week period and oral carriage of candida species, coliforms and staphylococcus aureus was assessed using an oral rinse technique. there was no significant diff ... | 1988 | 3293887 |
| evaluation of the in vitro susceptibility of gram-negative bacilli to cefotaxime, over a period of 3 years. | | 1988 | 3293972 |
| cefotaxime treatment of gram-negative enteric meningitis in infants and children. | 18 infants and children (1 week to 3 months of age) were treated with cefotaxime 200 mg/kg/day for gram-negative enteric bacillary meningitis. 17 of these patients (94.4%) survived, with a complication rate of 23.5% (4/17 patients). the follow-up cerebrospinal fluid cultures at 24 hours were sterile in all patients. cefotaxime was safe and effective in treating gram-negative enteric bacillary meningitis in infants and children and should be considered as a potential drug of choice in gram-negati ... | 1988 | 3293975 |
| comparison of serum concentrations of ceftazidime and tobramycin in newborn infants. | peak and trough serum concentrations of ceftazidime and tobramycin were determined in neonates with suspected septicaemia in an open randomized study. mean peak serum levels were 85 (+/- 4.4 se) mg/l for ceftazidime and 5.8 (+/- 0.3 se) mg/l for tobramycin. the peak serum levels of ceftazidime were well above the reported minimal inhibitory concentration (mic)90 values of pathogenic bacteria encountered in neonates, while peak serum levels of tobramycin were lower than reported mic90 values for ... | 1988 | 3294015 |
| evolution of beta-lactamase inhibitors. | beta-lactamase, the bacterial enzyme that can inactivate penicillins, cephalosporins and related antibiotics, can function outside the cell or in the periplasmic space. this resistance can be transferred between bacteria of the same or different species. most strains of staphylococcus aureus, staphylococcus epidermidis, klebsiella, enterobacter, pseudomonas aeruginosa and bacteroides species are beta-lactamase producers. clavulanic acid is a beta-lactamase inhibitor that works by blocking the en ... | 1988 | 3294401 |
| identification and characterization of two nitrogen fixation regulatory regions, nifa and nfrx, in azotobacter vinelandii and azotobacter chroococcum. | five tn5-induced nif- mutants of azotobacter vinelandii were characterized as regulatory mutants because they were restored to nif+ by the introduction of constitutively expressed nifa from klebsiella pneumoniae. the mutants fell into two different classes on the basis of hybridization to a rhizobium leguminosarum nifa gene probe and by complementation with cosmids isolated from plafri gene banks of a. vinelandii and azotobacter chroococcum. one mutant, mv3, was located in or near a nifa gene. t ... | 1988 | 3294559 |
| dose-dependent efficacy of vaccination against k. pneumoniae in high and low antibody responder lines of mice. | innate and acquired resistance to klebsiella pneumoniae infection were investigated in high (hi) and low (li) antibody responder lines of mice. the two lines were very susceptible to infection since even small inoculum doses of a virulent strain provoked a 100% mortality within a few days. however the mean survival time was significantly longer in li than in hi. (hi x li) f1 hybrids were more resistant than both parental lines. immunization with heat killed k. pneumoniae was able to confer full ... | 1987 | 3294582 |
| serum bactericidal activity against klebsiella pneumoniae in volunteers receiving increasing doses of tobramycin with or without cefamandole. | ten volunteers received intravenously in a randomly allocated order and on separate days the following regimens: cefamandole (15 mg/kg); tobramycin (1.5 mg/kg); tobramycin (3 mg/kg); cefamandole (15 mg/kg) + tobramycin (1.5 mg/kg); cefamandole (15 mg/kg) + tobramycin (3 mg-kg). tobramycin serum levels were 3.7 +/- 0.7 micrograms/ml one hour after infusion of the 1.5-mg/kg and 7.8 +/- 1.6 micrograms/ml after the higher dose. cefamandole serum levels were 26.0 +/- 5.2 micrograms/ml at the same tim ... | 1987 | 3294615 |
| postoperative acute acalculous cholecystitis. | a 5-year experience with postoperative acute acalculous cholecystitis is reported. the series concerns 9 male patients ranging in age from 28 to 69 years, with a mean age of 46 years. all underwent major surgical procedures and complications appeared in the postoperative course. clinically, 89% of patients developed sepsis and 66% jaundice. klebsiella pneumoniae was the microorganism most frequently isolated from the blood, intraabdominal and wound fluid collections. it is emphasized that the di ... | 1987 | 3294736 |
| efficacy of different dosing schedules of tobramycin for treating a murine klebsiella pneumoniae bronchopneumonia. | the efficacy of different dosing schedules of tobramycin for treating a murine klebsiella bronchopneumonia was compared. therapeutic efficacy depended upon dosing intervals. in mice treated for four days with a daily dose of 48 mg/kg, dosing intervals of 4 and 8 h allowed cure of 10/10 animals, whereas dosing intervals of 12 and 24 h yielded survival rates of only 6/10 (p less than 0.05) and 2/10 (p less than 0.01) respectively. although observed in vitro, a post-antibiotic effect of tobramycin ... | 1987 | 3294779 |
| in vitro transcription of the nitrogen fixation regulatory operon nifla of klebsiella pneumoniae. | in vitro transcription from the promoter for the nitrogen fixation regulatory operon nifla of k. pneumoniae requires four protein fractions: the core form of rna polymerase; ntra, an alternate sigma factor; ntrc, an auxiliary dna-binding protein; and ntrb, a bifunctional enzyme that controls the activity of ntrc by covalent modification (a.j. ninfa and b. magasanik, proc. natl. acad. sci. usa 83:5909, 1986). two dna-binding sites for ntrc lie approximately 150 base pairs upstream of the nifla pr ... | 1987 | 3294810 |
| common evolutionary origin of the ilvgmeda attenuation locus and trna(1leu) in escherichia coli. | published sequences of transcripts from ilvgmeda leader regions of several enteric bacteria were compared with published sequences of the trnas from escherichia coli. the analyses revealed homology between the ilvgmeda leader peptide-coding region and trna(1leu) in e. coli, salmonella typhimurium, and klebsiella aerogenes, whereas homology was not present in serratia marcescens and edwardsiella tarda. | 1987 | 3294812 |
| natural protective factors in bovine mammary secretions following different methods of milk cessation. | bovine mammary secretions were obtained during late lactation and early involution to determine if different methods of milk cessation influenced milk yield, composition, and in vitro growth of coliform mastitis pathogens. cows (n = 8/group) producing about 13 kg of milk prior to experimentation were dried off by abrupt or intermittent milk cessation. an additional group was dried off by intermittent milk cessation and fed only hay during the last week of lactation. cows milked intermittently pr ... | 1987 | 3294949 |
| biochemical characteristics of klebsiella pneumoniae derived from horses. | | 1987 | 3295349 |
| comparative efficacy of ceftazidime vs. carbenicillin and amikacin for treatment of neonatal septicemia. | the efficacy and safety of ceftazidime were compared with those of carbenicillin and amikacin in 60 neonates with proved invasive bacterial infections. the two treatment groups of patients were comparable with regard to sex, gestational and chronologic ages, associated risk factors, clinical condition on enrollment, focus of infection and bacteriology. escherichia coli was isolated from blood cultures of 31%, pseudomonas aeruginosa from cultures of 25%, klebsiella sp. from cultures of 13% and ot ... | 1987 | 3295738 |
| [epidemiological relation between klebsiella pneumoniae isolated from feces and from hospital urinary infections]. | | 1986 | 3296072 |
| [immunostimulant preparation made from the antigens of opportunistic microorganisms in the treatment of inflammatory lung diseases]. | the results of the trial of an immunostimulating preparation, consisting of klebsiella pneumoniae, proteus, escherichia coli and staphylococcus antigenic complexes, on 20 patients with acute pulmonary abscess and bronchiectasis are presented. the preparation was introduced subcutaneously in 5 injections. the preparation was found to have low reactogenicity, and in the course of immunotherapy the manifestations of systemic and local reactions became considerably less pronounced. immunotherapy pro ... | 1987 | 3296578 |
| [neonatal intrascrotal sepsis. apropos of 2 cases]. | | 1987 | 3296920 |
| quantum thermodynamics approach to phosphorylation and heterotrophic growth yields. | a model of cell growth is presented which is based on the double postulates of quantized loss of energy during phosphorylation and reversible biosynthesis of cell structure. an immediate consequence of the postulates is the identical value for the energy efficiency of the phosphorylation and for that of the whole growth process. another consequence is the relationship between the energy level of the biomass and the phosphorylation potential as embodied in the equation: eo = gamma'm x eatp, where ... | 1987 | 3297269 |
| incidence of staphylococcus aureus, coliforms and antibiotic-resistant strains of escherichia coli in rural water supplies in port harcourt. | the bacteriological quality of some rural water supplies in port harcourt was monitored over a 3 month period. the supplies were unsatisfactory as judged by standard plate counts (10(3)/ml) and the presence of presumptive and faecal coliforms and staphylococcus aureus. the recovery of potentially pathogenic bacteria (e.g. pseudomonas aeruginosa) further substantiated the existence of health hazards. the most frequently isolated coliforms were escherichia coli, enterobacter aerogenes and klebsiel ... | 1987 | 3298188 |
| activation and cleavage of the carbon-cobalt bond of adeninylethylcobalamin by diol dehydrase. | adeninylethylcobalamin (adeetcbl) underwent cleavage of the c-co bond by interaction with apoprotein of diol dehydrase from klebsiella pneumoniae atcc 8724, although this analog was quite inactive as coenzyme. spectroscopic observation indicates that adeetcbl was converted to the enzyme-bound hydroxocobalamin without intermediates. the conversion was stoichiometric (1:1) and obeyed the second-order reaction kinetics (k = 0.027 min-1 microm-1 at 37 degrees c) depending upon concentrations of apop ... | 1987 | 3298236 |
| nitrogenase mofe protein subunits from klebsiella pneumoniae expressed in foreign hosts. characteristics and interactions. | the expression of selected nitrogen fixation (nif) genes from klebsiella pneumoniae in foreign hosts provides an approach to determine the pathway, minimal genetic requirements, and host dependence of nitrogenase assembly. in this study, we investigated the assembly of the alpha 2 beta 2 mofe protein, responsible for substrate binding and reduction, by introducing nifd and nifk (encoding respectively, the alpha and beta subunits) into escherichia coli and the yeast saccharomyces cerevisiae. in e ... | 1987 | 3298241 |
| hla-b27, ankylosing spondylitis and klebsiella pneumoniae: toward a molecular approach. | | 1987 | 3298651 |
| utero-ovarian infection in aged b6c3f1 mice. | an unusually high number of ovarian masses and cysts with purulent material were observed in the b6c3f1 mice on 2 year chemical carcinogenicity studies sponsored by the national cancer institute-national toxicology program. to determine possible etiology, some of these lesions were cultured for bacteria and a majority yielded klebsiella sp. necropsy records of 14,029 female mice in 91 chronic studies necropsied from 1979 to 1983 at six toxicology testing laboratories were reviewed to determine t ... | 1987 | 3298846 |
| cultivation and synchronous morphogenesis of dictyostelium under controlled experimental conditions. | | 1987 | 3298997 |
| the journal of infection control nursing. on the right track. | | 1987 | 3299282 |
| effects of polysorbate 20 on bacterial growth. | | 1987 | 3299398 |
| [klebsiella pneumoniae mastitis as a dairying problem]. | quarter samples were taken from lactating and dry cows of a dairy herd in the netherlands. klebsiella pneumoniae was isolated from milk and dry udder secretum of cattle, and from the bedding of the cubicles. the animals concerned did not show any clinical symptoms. for some years klebsiella mastitis caused severe problems on this farm. a strict hygienic program resulted in complete control of the disease. diagnostics, therapy and prevention of klebsiella mastitis are discussed. | 1987 | 3299869 |
| haemagglutinins and adherence properties to hela and intestine 407 cells of klebsiella pneumoniae and klebsiella oxytoca isolates. | the occurrence of haemagglutination (ha) and adherence properties were examined in 50 strains of k. pneumoniae and 17 k. oxytoca strains isolated from humans. all isolates except three exhibited ha activity. mannose-sensitive haemagglutinins (msha) were expressed by the majority of k. pneumoniae strains, but only by one k. oxytoca isolate. mannose-resistant haemagglutination (mrha) to human or guinea pig erythrocytes could not be detected; haemagglutinins of the mr/k type were found in both spec ... | 1987 | 3300105 |
| oral norfloxacin versus parenteral treatment of nosocomial urinary tract infection. | in a multiclinic, randomized trial, oral norfloxacin, a fluoroquinolone antibacterial, was compared with several standard parenteral regimens for the treatment of nonbacteremic, hospital-acquired urinary tract infections. parenteral antibiotic agents included aminoglycosides alone; aminoglycosides in combination with either broad-spectrum penicillins or first-generation cephalosporins; or cefotaxime alone. ninety-two percent of bacterial isolates were multiresistant gram-negative rods including ... | 1987 | 3300312 |
| in vitro activity and beta-lactamase stability of a new monobactam, b0-1165. | b0-1165 is a 1-carboxy-1-cyclopropoxyamino,4-fluoromethyl monobactam. it inhibited the majority of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, aeromonas hydrophila, proteus mirabilis, proteus vulgaris, providencia rettgeri, providencia stuartii, yersinia enterocolitica, haemophilus influenzae, neisseria gonorrhoeae, and salmonella and shigella species at less than or equal to 0.125 microgram/ml. overall, its in vitro activity was similar to that of aztreona ... | 1987 | 3300528 |
| ciprofloxacin pharmacokinetics in patients with normal and impaired renal function. | the pharmacokinetics of ciprofloxacin following single oral doses of 500 and 750 mg in 32 patients with various degrees of renal function impairment were investigated in an open, randomized crossover fashion. ciprofloxacin was administered after overnight fasting; the washout time between the two doses was 1 week. serum and urine samples were collected serially between 0 and 24 h and subjected to bioassay and high-performance liquid chromatography. pharmacokinetic parameters were analyzed, assum ... | 1987 | 3300537 |
| sub-mics of cefuroxime and ciprofloxacin influence interaction of complement and immunoglobulins with klebsiella pneumoniae. | growth of encapsulated (k+) and nonencapsulated (k-) klebsiella pneumoniae strains in media containing sub-mics of either cefuroxime or ciprofloxacin resulted in cell elongation but had little effect on the outer membrane protein or lipopolysaccharide profiles. exposure to serum complement increased the surface hydrophobicity of a k- strain but failed to interact or to increase the surface hydrophobicity of the k+ strains. however, after growth of the k+ strains in sub-mics of the antibiotics, c ... | 1987 | 3300538 |
| biosynthesis of o-succinylbenzoic acid. i: cell free synthesis of o-succinylbenzoic acid from isochorismic acid in enzyme preparations from vitamin k producing bacteria. | escherichia coli k12 and a mutant of e. coli (viz., an 154) as well as aerobacter aerogenes 62-1 (i.e., klebsiella pneumoniae) were used as sources of the enzyme catalyzing the formation of o-succinylbenzoic acid (osb) from isochorismic acid and alpha-ketoglutaric acid in the presence of thiamine pyrophosphate. the product of the reaction (osb) was identified by hplc before and after derivatization to the methylester, dilactone, and coenzyme a ester. osb synthase and alpha-ketoglutarate dehydrog ... | 1987 | 3300552 |
| diclofenac binding to human polymorphonuclear neutrophils: effect on respiratory burst and n-formylated peptide binding. | the respiratory burst of human polymorphonuclear neutrophils (pmn) induced by particle or soluble stimuli was measured in the presence of the nonsteroidal anti-inflammatory drug, diclofenac sodium (voltaren). diclofenac (25-100 micrograms/ml) inhibited the oxygen consumption of pmn stimulated by 5 x 10(-7) m of n-formyl-methionyl-leucyl-phenylalanine (fmlp). the inhibition was linearly correlated to diclofenac concentration. by contrast, diclofenac did not affect the rate of heat-killed klebsiel ... | 1987 | 3300657 |
| response to the editorial by terasaki and yu on the ankylosing spondylitis/klebsiella/hla-b27 problem. | | 1987 | 3300661 |
| the resemblance of clinical attributes between mastitic cows with no growth on bacterial milk cultures and those with gram-positive bacteria cultured. | the clinical attributes of 40 dairy cows which had mastitis but no growth of bacteria from the milk were analyzed and compared to the attributes in 102 cows with only gram-positive and 61 cows with only gram-negative bacteria cultured from the milk. cows with no bacteria cultured from the milk did not differ significantly from cows with gram-positive bacteria cultured, but 9 of 12 attributes were significantly different between cows with no bacteria cultured and cows with gram-negative bacteria ... | 1987 | 3300920 |
| the sera of patients with klebsiella infections contain a common anti-dna idiotype (16/6) id and anti-polynucleotide activity. | in view of recent reports linking klebsiella pneumoniae with autoimmunity, we have examined the sera of 52 patients with urinary tract infection or septicaemia from this gram-negative pathogen, for the presence of antibodies to dna, polynucleotides, cardiolipin and a common anti-dna idiotype 16/6. up to 27% of these patients had anti-polynucleotide antibodies detectable, and in 37% the 16/6 idiotype was found. absorption of the sera of two patients, with no dna binding, against the klebsiella po ... | 1987 | 3301096 |
| profile of autoantibodies in the serum of patients with tuberculosis, klebsiella and other gram-negative infections. | autoantibody profiles were examined in the sera of untreated patients with tuberculosis, and those with klebsiella septicaemia, and klebsiella and e. coli urinary tract infections. rheumatoid factors of the igm, iga and igg isotypes, antinuclear antibodies and antibodies to poly(adp-ribose) were all frequently detected (generally 15-40%). in contrast, antibodies to the extractable nuclear antigens and to the organ specific antigens were unusual (generally less than 10%). in comparison, in a grou ... | 1987 | 3301097 |
| characterization of klebsiella pneumoniae from environmental sources. | | 1987 | 3301652 |
| [incidence of deep respiratory tract infections]. | the distribution of respiratory tract infections (rti) among the general population is not uniform. the incidence in neonates and the elderly (older than 65) is 2 to 3 times higher than that in adults. examinations to determine the responsible pathogen are conducted in less than 1% of cases of rti. the overall incidence of haemophilus influenzae and streptococcus pneumoniae in hospitalized patients amounts to 13 to 27%. the incidences of staphylococcus aureus, klebsiella and pseudomonas aerugino ... | 1987 | 3301684 |
| the mechanism of action of piperacillin-analogues in vitro; effect of the carbon number at the n-4 position of 2,3-dioxopiperazine on the outer membrane permeability, stability to beta-lactamase and binding affinity to penicillin-binding proteins. | the relationship between the chemical structure and the mode of action of piperacillin-analogues (pipc-analogues) against escherichia coli and klebsiella pneumoniae were investigated. the antibacterial activity of pipc-analogues increased with an increase in the number of carbon atoms at the n-4 position of 2,3-dioxopiperazine. their mode of action is discussed on the basis of the results of studies on outer membrane permeability, stability to beta-lactamase and binding affinity to penicillin-bi ... | 1987 | 3301774 |
| limitations of the a-1m method for fecal coliform enumeration in the pacific oyster (crassostrea gigas). | use of the a-1m method, which was originally devised for testing water samples, has recently been extended for enumeration of fecal coliforms and escherichia coli in shellfish and other food products. results of our study indicate that while this method is reliable for analysis of growing waters, the use of the a-1m method for testing pacific oysters may be less reliable because bacteria not belonging to the coliform group but which are sometimes present in these animals also give a positive rea ... | 1987 | 3301793 |
| new bacteriophage typing scheme for subdivision of the frequent capsular serotypes of klebsiella spp. | a bacteriophage typing scheme for hospital isolates of klebsiella spp. was developed. the scheme was designed specifically as a secondary typing method to discriminate between strains of serotypes k2, k3, and k21 but proved to be an efficient general typing method for strains of most serotypes. the set of 15 phages gave 87.3% typeability on 236 strains of more than 70 different serotypes. typeability within the k2, k3, and k21 strains was 93, 89, and 91%, respectively. there was a mean of 3.2 re ... | 1987 | 3301889 |
| bacterial adhesion to cerebrospinal fluid shunts. | bacterial adherence to cerebrospinal fluid (csf) shunts was analyzed in vivo and in vitro. scanning electron micrographs (sem's) of catheters removed from pediatric patients with shunts infected by staphylococcus aureus or klebsiella pneumoniae revealed numerous bacterial cells and microcolonies, leukocytes, and erythrocytes attached to the csf catheters' inner walls, as well as the existence of surface irregularities, such as fissures, rugosities, and holes. permeability analyses and sem's demo ... | 1987 | 3302132 |
| frameshifts close to the klebsiella pneumoniae nifh promoter prevent multicopy inhibition by hybrid nifh plasmids. | certain multicopy plasmids bearing promoter sequences of nitrogen fixation (nif) genes inhibit expression of chromosomal genes in nif+ klebsiella pneumoniae, hence leading to a nif- phenotype. this 'multicopy inhibition' has been attributed to the titration of the nif-specific activator protein nifa by the plasmid-borne promoter sequences. we now report that multicopy inhibition by nifh translational fusions is sensitive to frameshifts close to the nifh promoter. transcriptional nifh fusion plas ... | 1987 | 3302601 |
| [comparative antibacterial activity of a new monobactam, carumonam (ro 17-2301, ama 1080) on hospital bacteria resistant to beta lactams]. | in vitro activity of a new monobactam, carumonam (ro 17-2301, ama 1080) was tested against 370 hospital bacterial isolates. results were compared to aztreonam, cefotaxime, cefmenoxime, latamoxef, ceftazidime, ceftriaxone, pefloxacin against enterobacteriaceae, to aztreonam, piperacillin, cefoperazone, cefsulodin, ceftazidime, imipenem and pefloxacin against p. aeruginosa. all enterobacteriaceae strains produced cephalosporinases and all p. aeruginosa strains were ticarcillin resistant. mic 90% o ... | 1987 | 3302846 |
| [activity of ticarcillin combined with different concentrations of clavulanic acid on 137 gram-negative bacilli resistant to ticarcillin]. | the mic of ticarcillin exclusively or joined with 3 concentrations of clavulanic acid: (2, 4 and 8 mg/l) was determined by agar dilution in relation to 137 bacilli resistant to ticarcillin (mic greater than or equal to 256 mg/l) detected between 1985 and 1986 in the bellevue hospital in saint-etienne. we could study 26 escherichia coli, 30 klebsiella, 28 enterobacter, 36 serratia and 17 pseudomonas aeruginosa. the concentration of 2 mg/l of clavulanic acid allows to lower the mic of ticarcillin ... | 1987 | 3302858 |
| [bone infections: treatment by ofloxacin. apropos of 10 cases]. | because of its pharmacokinetic, broad spectrum and oral administration, ofloxacin can be used in the treatment of chronic bone infections. a clinical trial was performed in 10 patients with subacute or chronic osteitis (5 staphylococcus aureus, 1 staphylococcus epidermidis, 1 klebsiella oxytoca, 1 escherichia coli, 1 serratia marcescens, 1 pseudomonas aeruginosa). patients were given orally 200 mg 12 hourly. treatment duration went on from 2 to 6 months. in this trial, the evaluation was success ... | 1987 | 3302877 |
| [treatment of febrile episodes in neutropenic children by ceftazidime combined with netilmicin. results of a multicenter study apropos of 88 cases]. | infection is the most important cause of mortality in leucopenic patients. a broad spectrum antibiotic therapy is imperative in febrile and neutropenic patients. in a multicentric study we have used ceftazidime (100 mg/kg/d) and netilmicin (6 mg/kg/d) in 88 children (fever greater than or equal to 38.5 degrees c, neutropenia less than 500/mm3) treated for acute leukemias (59), non hodgkin lymphomas (13) or solid tumors (16). median age was 7 years (2 months-16 years). in patients who continued t ... | 1987 | 3302878 |
| alveolar destruction in experimental klebsiella pneumonia. | sequential histological changes of the lungs were studied in experimental klebsiella pneumonia, using untreated control mice, cyclophosphamide-treated mice, and carrageenan-treated mice. cyclophosphamide was used to deplete polymorphonuclear leukocytes and monocytes, and carrageenan was used to deplete mononuclear phagocytes selectively. at 72 hours, varying degree of alveolar necrosis could be seen in untreated control mice. however, the lung lesions of cyclophosphamide- or carrageenan-treated ... | 1987 | 3303826 |
| electron microscopical investigation of citrate lyase single molecules. | electron micrographs of citrate lyase from rhodopseudomonas gelatinosa and klebsiella aerogenes reveal two characteristic molecular forms. the "basket" form and the "star" form were subjected to two-dimensional image reconstruction using a technique involving averaging of superposed single molecular images after rotational correlation. a three-dimensional image reconstruction shows that the images of these forms can be interconverted by rotation and that they therefore represent different views ... | 1987 | 3304340 |
| growth of escherichia coli in a pulp and cardboard mill. | the coliform flora of a pulp and cardboard mill that uses birch as the raw material and ammonium sulphate as the process chemical was studied. escherichia coli was observed to multiply in the mill. it persisted as the dominant thermotolerant coliform in the effluent. klebsiellae were encountered among total coliforms only. the e. coli strains isolated had the biochemical characteristics and maximum growth temperatures typical to the species. however, serotyping and hemolysin test differentiated ... | 1987 | 3304598 |
| ligand-binding and idiotypic cross-reactions between anti-dna antibodies and antibodies to klebsiella k30 polysaccharide in patients with systemic lupus erythematosus or rheumatoid arthritis. | antibodies to dna and k30p were purified by affinity procedures and tested for their ability to cross-react with k30p and dna, respectively. anti-ssdna antibodies were shown to react with solid-phase k30p and be inhibited in a dose-dependent manner by soluble ssdna and k30p. conversely, anti-k30p antibodies were found to bind immobilized ssdna and be inhibited with soluble inhibitor. these results show that certain subpopulations of anti-ssdna and anti-k30p antibodies overlap in their ligand-bin ... | 1987 | 3304739 |
| serological studies in crohn's disease. | elisa and immunofluorescence tests were carried out on sera from patients with crohn's disease and ulcerative colitis. the results indicate that mean antibody levels to yersinia enterocolitica types o:9 and o:3 and klebsiella pneumoniae, but not pseudomonas maltophilia, are significantly higher in these patients than in control subjects. there are differing antibody levels in ileal and colonic disease, and there is a significant fall in antibody levels 9-12 months after remission from clinical s ... | 1987 | 3305007 |
| purification of the lipopolysaccharide fraction from klebsiella pneumoniae o1 k2 by high-performance liquid chromatography. | an high-performance liquid chromatography technique was applied to purify the lipopolysaccharide fraction from a lysate of klebsiella pneumoniae o1 k2 (nctc 5055). the separation of the lipopolysaccharide fraction from the proteins was carried out with a reversed-phase column. by this method the lipopolysaccharide fraction was obtained in a pure state, devoid of proteins but possessing the same biological properties as the lipopolysaccharide fraction prepared by the classical phenol-water techni ... | 1987 | 3305533 |
| cephalosporin, carbapenem, and monobactam antibiotics. | cephalosporin and related antibiotics are highly effective bactericidal agents of relatively low toxicity. the spectrum of activity varies with the drug but is usually broad. the first-generation cephalosporins, and especially cefazolin, are most active against sensitive staphylococci and streptococci. most second-generation (except cefoxitin) and third-generation cephalosporins show substantial activity against haemophilus influenzae. all cephalosporins (except cefsulodin) are active against kl ... | 1987 | 3306182 |
| [resistance of urinary flora from sources outside the hospital]. | | 1987 | 3306193 |
| experimental inoculation of klebsiella rhinoscleromatis bacilli in albino mice. | 72 albino mice were intranasally inoculated with klebsiella rhinoscleromatis suspension with or without the addition of hog mucin. scleroma-like lesions developed in their lungs more frequently with hog mucin. a liver affection was detected only in 1 animal. k. rhinoscleromatis organisms were recovered from the lung lesions. a possible potentiating effect of hog mucin is discussed. | 1987 | 3306554 |
| [transference and resistance to serum in gram-negative strains multiresistant to antimicrobial drugs]. | | 1987 | 3306858 |
| gentamicin resistance among gram-negative bacillary blood isolates in a hospital with long-term use of gentamicin. | between 1977 and 1985, gentamicin was the only formulary aminoglycoside at the buffalo veterans administration medical center. during this time, there was a significant increase in the amount of gentamicin purchased. amikacin represented 11% or less of the total aminoglycoside purchased in the same period, but purchases of this agent also significantly increased. because of this long-term use of gentamicin, a retrospective analysis of gentamicin resistance among gram-negative bacillary blood iso ... | 1987 | 3307673 |
| the k1 beta-lactamase of klebsiella pneumoniae. | beta-lactamase k1 was purified from klebsiella pneumoniae sc10436. it is very similar to the enzyme produced by klebsiella aerogenes 1082e and described by emanuel, gagnon & waley [biochem. j. (1986) 234, 343-347]. an active-site peptide was isolated after labelling of the enzyme with tritiated beta-iodopenicillanate. a cysteine residue was found just before the active-site serine residue. this result could explain the properties of the enzyme after modification by thiol-blocking reagents. the s ... | 1987 | 3307765 |
| ru-41740 (k. pneumoniae glycoprotein) enhances resistance to experimental candidiasis and stimulates phagocytic functions. | ru-41740, a purified glycoprotein extract from klebsiella pneumoniae, (which is an efficient non-specific immune activator in a broad spectrum of in vitro and in vivo reactions) was administered either orally or parenterally in the mouse. it enhanced the resistance of mice to candidiasis, both in terms of survival rate and a decrease in viable yeast cell recovery in kidneys. the drug administered at 0.1 mg or 1 mg/kg augmented 4-fold the mean survival time (mst) of animals infected with 1 to 2 x ... | 1987 | 3307833 |
| suspected infectious agent and host environment interactions in spondyloarthropathies. | | 1987 | 3308235 |
| raised serum levels of cachectin/tumor necrosis factor alpha in renal allograft rejection. | a sensitive radioimmunoassay was used for monitoring serum levels of endogenous cachectin/tumor necrosis factor alpha (tnf) in 10 renal transplant recipients. acute allograft rejections were associated with marked elevations of circulating tnf. the peak levels of tnf (median 140 pg/ml) were in the same concentration range as previously reported in parasitic infections. the results show that the release of tnf into circulation is an early event in renal allograft rejection and that raised levels ... | 1987 | 3309124 |
| isolation from klebsiella and characterization of two rcs genes that activate colanic acid capsular biosynthesis in escherichia coli. | two genes, designated rcsa (regulation of capsule synthesis) and rcsb, that had been cloned from the chromosome of klebsiella aerogenes (k. pneumoniae) capsular serotype k21 were capable of activating expression of colanic acid capsular polysaccharide in escherichia coli k12. the klebsiella rcsa gene encoded a polypeptide of 23 kda that was required for the induction of a mucoid phenotype at less than or equal to 30 degrees c but not at greater than or equal to 37 c. the klebsiella rcsb locus en ... | 1987 | 3309150 |